AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022003.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022003.01.T01 MTR_4g080360 89.181 342 37 0 1 342 1 342 0.0 637
MsG0480022003.01.T01 MTR_2g083930 63.422 339 114 5 8 342 12 344 2.54e-154 437
MsG0480022003.01.T01 MTR_4g080730 61.846 325 116 5 20 342 22 340 2.71e-145 414
MsG0480022003.01.T01 MTR_4g080700 59.475 343 131 5 2 342 4 340 2.34e-143 409
MsG0480022003.01.T01 MTR_5g022560 52.802 339 155 4 7 342 8 344 1.38e-129 374
MsG0480022003.01.T01 MTR_4g047610 53.727 322 143 4 24 342 25 343 1.93e-127 369
MsG0480022003.01.T01 MTR_4g107930 51.585 347 161 5 1 342 1 345 1.32e-126 367
MsG0480022003.01.T01 MTR_4g079770 52.959 338 152 5 9 342 11 345 3.56e-126 365
MsG0480022003.01.T01 MTR_4g079800 54.180 323 141 5 24 342 26 345 1.29e-125 364
MsG0480022003.01.T01 MTR_4g079470 54.489 323 140 5 24 342 26 345 3.60e-124 360
MsG0480022003.01.T01 MTR_2g075830 54.599 337 145 4 9 342 10 341 9.25e-124 359
MsG0480022003.01.T01 MTR_7g078600 56.592 311 122 6 33 341 38 337 4.77e-118 345
MsG0480022003.01.T01 MTR_3g075390 50.955 314 150 3 30 341 33 344 3.28e-114 336
MsG0480022003.01.T01 MTR_7g079230 53.994 313 126 6 33 340 35 334 5.67e-114 334
MsG0480022003.01.T01 MTR_4g104370 50.318 314 151 4 30 341 33 343 6.74e-110 325
MsG0480022003.01.T01 MTR_1g107550 48.718 312 155 4 33 341 46 355 4.37e-104 314
MsG0480022003.01.T01 MTR_4g094918 46.567 335 172 6 11 341 10 341 2.52e-102 305
MsG0480022003.01.T01 MTR_4g079440 47.097 310 156 5 33 341 42 344 2.26e-100 300
MsG0480022003.01.T01 MTR_3g116080 48.515 303 152 4 38 340 48 346 9.37e-100 298
MsG0480022003.01.T01 MTR_2g090890 43.529 340 178 6 8 341 5 336 3.55e-99 296
MsG0480022003.01.T01 MTR_3g102220 44.693 358 175 8 3 341 8 361 2.43e-97 296
MsG0480022003.01.T01 MTR_1g018840 46.926 309 158 5 37 341 55 361 4.56e-96 293
MsG0480022003.01.T01 MTR_1g018840 46.926 309 158 5 37 341 55 361 6.70e-96 293
MsG0480022003.01.T01 MTR_2g015850 51.034 290 139 3 52 341 77 363 2.68e-94 285
MsG0480022003.01.T01 MTR_3g112430 46.405 306 161 2 38 341 37 341 2.34e-92 283
MsG0480022003.01.T01 MTR_2g067900 45.970 335 155 13 24 341 1 326 1.41e-80 249
MsG0480022003.01.T01 MTR_2g016180 42.208 308 168 6 38 340 52 354 1.13e-77 246
MsG0480022003.01.T01 MTR_2g016270 41.234 308 156 7 38 340 52 339 4.91e-76 241
MsG0480022003.01.T01 MTR_2g064475 41.226 359 157 14 24 339 1 348 2.05e-75 236
MsG0480022003.01.T01 MTR_7g078630 48.611 288 98 7 29 301 698 950 1.81e-72 242
MsG0480022003.01.T01 MTR_2g066980 40.395 354 153 14 6 340 3 317 1.07e-70 223
MsG0480022003.01.T01 MTR_2g067560 42.169 332 161 13 24 334 1 322 1.13e-67 228
MsG0480022003.01.T01 MTR_2g015920 37.228 368 194 14 1 341 1 358 5.84e-66 213
MsG0480022003.01.T01 MTR_4g065077 36.236 356 198 12 7 341 9 356 6.04e-64 207
MsG0480022003.01.T01 MTR_1g023810 46.544 217 110 4 125 339 49 261 8.46e-63 201
MsG0480022003.01.T01 MTR_2g015930 37.808 365 189 17 1 337 3 357 2.27e-62 203
MsG0480022003.01.T01 MTR_4g125300 37.879 330 178 13 24 341 34 348 1.60e-61 200
MsG0480022003.01.T01 MTR_8g086470 40.824 267 144 8 81 341 98 356 5.91e-57 189
MsG0480022003.01.T01 MTR_4g071060 35.179 307 171 9 43 333 54 348 7.37e-54 181
MsG0480022003.01.T01 MTR_1g023210 34.740 308 174 10 42 333 56 352 1.49e-51 175
MsG0480022003.01.T01 MTR_1g094135 33.754 317 182 11 36 335 55 360 2.10e-44 157
MsG0480022003.01.T01 MTR_4g072790 33.220 295 157 11 43 333 54 312 5.44e-40 144
MsG0480022003.01.T01 MTR_7g078580 61.224 98 37 1 244 341 5 101 4.45e-38 132
MsG0480022003.01.T01 MTR_4g072340 30.872 298 164 9 43 333 54 316 7.47e-38 138
MsG0480022003.01.T01 MTR_4g073660 30.872 298 164 9 43 333 53 315 1.38e-37 137
MsG0480022003.01.T01 MTR_7g079130 62.637 91 34 0 251 341 7 97 6.66e-37 128
MsG0480022003.01.T01 MTR_1g025450 40.741 189 90 5 111 296 41 210 3.45e-35 135
MsG0480022003.01.T01 MTR_2g015840 40.268 149 88 1 12 159 7 155 2.43e-33 124
MsG0480022003.01.T01 MTR_2g015840 49.153 118 59 1 223 340 154 270 1.01e-32 123
MsG0480022003.01.T01 MTR_7g078310 47.101 138 61 3 81 215 117 245 4.48e-30 115
MsG0480022003.01.T01 MTR_2g016380 45.082 122 61 4 222 340 16 134 9.69e-27 106
MsG0480022003.01.T01 MTR_3g029560 32.536 209 116 7 137 341 50 237 1.80e-25 102
MsG0480022003.01.T01 MTR_7g111050 29.856 278 151 11 86 333 64 327 8.79e-22 95.1
MsG0480022003.01.T01 MTR_7g111060 26.752 314 188 13 52 333 27 330 4.14e-21 93.2
MsG0480022003.01.T01 MTR_7g079260 51.389 72 19 1 267 338 7 62 2.43e-17 77.0
MsG0480022003.01.T01 MTR_2g047960 42.169 83 48 0 134 216 7 89 6.40e-16 72.8
MsG0480022003.01.T01 MTR_3g082370 24.519 208 136 7 129 320 4 206 1.51e-12 66.6
MsG0480022003.01.T01 MTR_7g111050 27.419 248 140 10 86 303 59 296 1.02e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022003.01.T01 AT5G50260 49.709 344 160 4 9 341 1 342 4.20e-118 345
MsG0480022003.01.T01 AT5G45890 47.941 340 167 7 8 340 8 344 9.61e-111 326
MsG0480022003.01.T01 AT2G34080 45.690 348 180 5 1 342 1 345 1.04e-109 323
MsG0480022003.01.T01 AT2G27420 45.739 352 177 5 1 342 1 348 1.57e-108 321
MsG0480022003.01.T01 AT3G49340 46.154 338 166 5 18 342 7 341 3.11e-104 310
MsG0480022003.01.T01 AT1G29090 45.349 344 177 6 7 342 15 355 3.59e-102 305
MsG0480022003.01.T01 AT1G29080 42.566 343 188 5 7 342 6 346 5.06e-101 301
MsG0480022003.01.T01 AT1G20850 48.693 306 149 4 38 340 52 352 6.12e-101 301
MsG0480022003.01.T01 AT3G19390 43.228 347 187 4 1 340 1 344 7.24e-101 305
MsG0480022003.01.T01 AT3G48340 45.428 339 178 5 7 341 8 343 4.55e-100 300
MsG0480022003.01.T01 AT3G19400 48.534 307 152 6 37 340 44 347 1.55e-99 298
MsG0480022003.01.T01 AT4G35350 48.185 303 154 3 38 340 52 351 3.97e-99 297
MsG0480022003.01.T01 AT3G48350 47.557 307 156 4 37 340 38 342 7.82e-99 296
MsG0480022003.01.T01 AT1G06260 47.720 329 147 8 24 341 28 342 3.81e-96 289
MsG0480022003.01.T01 AT5G43060 45.631 309 159 4 37 341 50 353 3.53e-95 291
MsG0480022003.01.T01 AT1G47128 45.779 308 159 6 37 341 50 352 7.89e-95 290
MsG0480022003.01.T01 AT1G09850 43.689 309 171 2 33 341 28 333 8.58e-92 281
MsG0480022003.01.T01 AT4G11310 44.300 307 164 6 38 341 50 352 2.55e-89 272
MsG0480022003.01.T01 AT4G23520 42.655 354 177 10 7 341 3 349 3.11e-89 272
MsG0480022003.01.T01 AT1G29110 40.491 326 172 6 21 341 25 333 1.96e-88 269
MsG0480022003.01.T01 AT4G11320 43.831 308 164 7 38 341 57 359 3.46e-88 270
MsG0480022003.01.T01 AT4G11320 43.831 308 164 7 38 341 57 359 3.46e-88 270
MsG0480022003.01.T01 AT4G36880 46.259 294 152 4 53 341 69 361 7.23e-88 269
MsG0480022003.01.T01 AT3G43960 45.484 310 160 7 37 341 41 346 8.59e-88 269
MsG0480022003.01.T01 AT3G48350 47.521 242 122 4 37 275 38 277 1.34e-69 219
MsG0480022003.01.T01 AT3G19400 46.835 237 121 5 37 270 44 278 3.24e-69 218
MsG0480022003.01.T01 AT4G35350 45.532 235 126 2 38 272 52 284 1.09e-67 214
MsG0480022003.01.T01 AT5G60360 40.193 311 163 12 39 341 61 356 5.20e-64 207
MsG0480022003.01.T01 AT5G60360 39.871 311 163 13 39 341 61 355 2.23e-61 200
MsG0480022003.01.T01 AT3G45310 37.651 332 180 13 22 341 40 356 1.85e-60 198
MsG0480022003.01.T01 AT5G60360 39.073 302 161 12 39 332 61 347 7.83e-59 194
MsG0480022003.01.T01 AT3G45310 37.349 332 180 14 22 341 40 355 7.09e-58 191
MsG0480022003.01.T01 AT4G16190 36.129 310 173 10 42 335 60 360 7.45e-54 181
MsG0480022003.01.T01 AT2G21430 35.577 312 164 11 43 333 54 349 2.14e-52 177
MsG0480022003.01.T01 AT4G39090 34.528 307 174 9 43 333 57 352 2.08e-51 175
MsG0480022003.01.T01 AT3G54940 32.424 330 191 10 26 333 35 354 3.83e-46 161
MsG0480022003.01.T01 AT3G54940 30.769 247 153 6 25 257 34 276 8.05e-33 123
MsG0480022003.01.T01 AT4G01610 28.105 306 169 12 66 337 46 334 3.27e-24 102
MsG0480022003.01.T01 AT1G02305 29.126 309 164 14 60 333 45 333 1.56e-23 100
MsG0480022003.01.T01 AT4G01610 27.778 306 170 12 66 337 46 334 5.83e-23 98.6
MsG0480022003.01.T01 AT1G02300 27.570 214 122 7 144 333 146 350 4.75e-17 81.6

Find 65 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAACTAATGAAGAATTTA+AGG 0.104866 4:-66970074 MsG0480022003.01.T01:CDS
CTAGTTCACCTGTTGTTATT+TGG 0.195528 4:+66968825 None:intergenic
GAATTTAAGGCTTCACTTAA+TGG 0.220317 4:-66970061 MsG0480022003.01.T01:CDS
TACCCTTTAATCTCAGTTAC+AGG 0.251082 4:+66968626 None:intergenic
TATTTGGTGGATACTTTCTA+TGG 0.264591 4:+66968841 None:intergenic
CATTAGTTTGGTCGGCAAAA+TGG 0.272975 4:+66970086 None:intergenic
GTCTCGTCTGTTTCTTTGTA+AGG 0.275381 4:+66968674 None:intergenic
AAGCCTGTAACTGAGATTAA+AGG 0.279226 4:-66968629 MsG0480022003.01.T01:CDS
TATGTTGATGCAGGAAGTTC+TGG 0.293708 4:-66968536 MsG0480022003.01.T01:CDS
CCTTAAATTCTTCATTAGTT+TGG 0.299951 4:+66970074 None:intergenic
AAGAAGTTATATAATGAAAT+TGG 0.301794 4:-66970034 MsG0480022003.01.T01:CDS
ATAGGTATAATAAAATTGTT+TGG 0.323262 4:+66970328 None:intergenic
TAGGTATAATAAAATTGTTT+GGG 0.351516 4:+66970329 None:intergenic
GTTGTTACTGTGGTTGGTTA+TGG 0.359399 4:-66968455 MsG0480022003.01.T01:CDS
TTCCTCCAATCCATGTTAGT+TGG 0.379264 4:+66969962 None:intergenic
CTCATGTTTATTTGACAAGC+AGG 0.401311 4:+66970246 None:intergenic
GGTACTGGGTGGGGTGAGAA+AGG 0.417232 4:-66968386 MsG0480022003.01.T01:CDS
ATTGAATCGTTCAATGCTGT+TGG 0.434640 4:-66970136 MsG0480022003.01.T01:CDS
AGATAATAGCATTAAGCAAC+GGG 0.441105 4:+66968293 None:intergenic
ATGGTTAATACTAAGGTTGA+AGG 0.443195 4:+66970105 None:intergenic
AGCCTGTAACTGAGATTAAA+GGG 0.447044 4:-66968628 MsG0480022003.01.T01:CDS
CACATGATTATACTGAGTAT+TGG 0.448386 4:-66968427 MsG0480022003.01.T01:CDS
TGTCAAATAAACATGAGAAA+TGG 0.448853 4:-66970240 MsG0480022003.01.T01:CDS
CGGCAAAATGGTTAATACTA+AGG 0.458456 4:+66970098 None:intergenic
AATTCATGGGGTACTGGGTG+GGG 0.464045 4:-66968395 MsG0480022003.01.T01:CDS
TCCCTCATGTAATGTCTAGC+AGG 0.464408 4:-66970279 MsG0480022003.01.T01:CDS
GCATCAACATAAACTGATAC+AGG 0.465680 4:+66968548 None:intergenic
TTGTGAAAATAGGTAGTTGT+TGG 0.466035 4:-66968883 MsG0480022003.01.T01:intron
GTTCACCTGTTGTTATTTGG+TGG 0.467347 4:+66968828 None:intergenic
TGTACGTACCACATGATCCT+TGG 0.481588 4:+66969912 None:intergenic
CGAGACATGCAAAGTTAAGA+AGG 0.493107 4:-66968657 MsG0480022003.01.T01:CDS
TGTGAAAATAGGTAGTTGTT+GGG 0.493321 4:-66968882 MsG0480022003.01.T01:intron
GTCGCTATCAGTACAAGAAC+TGG 0.496349 4:-66968801 MsG0480022003.01.T01:CDS
AAATTCATGGGGTACTGGGT+GGG 0.497402 4:-66968396 MsG0480022003.01.T01:CDS
AGTATCCACCAAATAACAAC+AGG 0.500325 4:-66968833 MsG0480022003.01.T01:CDS
CATTAAGCAACGGGATAAGA+AGG 0.506027 4:+66968302 None:intergenic
AAATTCTTCATTAGTTTGGT+CGG 0.509889 4:+66970078 None:intergenic
GTATCAGTTTATGTTGATGC+AGG 0.516979 4:-66968545 MsG0480022003.01.T01:CDS
GATCATGTTGTTACTGTGGT+TGG 0.519645 4:-66968461 MsG0480022003.01.T01:CDS
GTACTGGGTGGGGTGAGAAA+GGG 0.525057 4:-66968385 MsG0480022003.01.T01:CDS
ATCCATGTTAGTTGGAACAT+AGG 0.527461 4:+66969970 None:intergenic
TCTCGTCTGTTTCTTTGTAA+GGG 0.531535 4:+66968675 None:intergenic
GTTGTGAAAATGAGAAACAT+AGG 0.532455 4:+66970310 None:intergenic
AACATGGATTGGAGGAAAAG+AGG 0.535704 4:-66969956 MsG0480022003.01.T01:CDS
ATGTTCCAACTAACATGGAT+TGG 0.537686 4:-66969967 MsG0480022003.01.T01:CDS
TTATAAAGATGCCGCAGAGA+AGG 0.551341 4:-66970197 MsG0480022003.01.T01:CDS
TGGTTAATACTAAGGTTGAA+GGG 0.555566 4:+66970106 None:intergenic
TTCCAACTAACATGGATTGG+AGG 0.558122 4:-66969964 MsG0480022003.01.T01:CDS
AGCAACGGGATAAGAAGGAT+TGG 0.561863 4:+66968307 None:intergenic
CCCTCATGTAATGTCTAGCA+GGG 0.564256 4:-66970278 MsG0480022003.01.T01:CDS
TCATGTTTATTTGACAAGCA+GGG 0.571499 4:+66970247 None:intergenic
TGTGTGAGAGGTAATAGCAG+TGG 0.577269 4:-66968773 MsG0480022003.01.T01:CDS
AAAATTCATGGGGTACTGGG+TGG 0.589534 4:-66968397 MsG0480022003.01.T01:CDS
CAGATAATAGCATTAAGCAA+CGG 0.590427 4:+66968292 None:intergenic
AACGGGATAAGAAGGATTGG+TGG 0.605158 4:+66968310 None:intergenic
AACCTATGTTCCAACTAACA+TGG 0.605905 4:-66969972 MsG0480022003.01.T01:CDS
AATAGCAGTGGATGCAGTGG+TGG 0.606478 4:-66968761 MsG0480022003.01.T01:CDS
GAACTGGTAGATTGTGTGAG+AGG 0.607883 4:-66968785 MsG0480022003.01.T01:CDS
TCAAATATTCAAGAACAATG+TGG 0.615323 4:-66970164 MsG0480022003.01.T01:CDS
AAATGATCTTCTAAAAGCTG+TGG 0.616862 4:-66968579 MsG0480022003.01.T01:CDS
AGGTATAATAAAATTGTTTG+GGG 0.625361 4:+66970330 None:intergenic
GGTAATAGCAGTGGATGCAG+TGG 0.652696 4:-66968764 MsG0480022003.01.T01:CDS
ACCCTGCTAGACATTACATG+AGG 0.657699 4:+66970277 None:intergenic
CCCTGCTAGACATTACATGA+GGG 0.666413 4:+66970278 None:intergenic
TACAGATCATGTTGTTACTG+TGG 0.670860 4:-66968465 MsG0480022003.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTCTTATTATAATATATATT+TGG + Chr4:66968762-66968781 None:intergenic 10.0%
!! GATTAAATAAAATTTATACA+TGG - Chr4:66969106-66969125 MsG0480022003.01.T01:intron 10.0%
!! GCTATATAAAAAAAATATTA+TGG + Chr4:66969572-66969591 None:intergenic 10.0%
!!! TAATTATTAGCTTTTTTTTT+TGG - Chr4:66969220-66969239 MsG0480022003.01.T01:intron 10.0%
!!! TTTTTTAGCTAATAATTATA+GGG + Chr4:66969365-66969384 None:intergenic 10.0%
!!! TTTTTTTAGCTAATAATTAT+AGG + Chr4:66969366-66969385 None:intergenic 10.0%
!! AAAAAACTATTACATAATGA+TGG - Chr4:66969382-66969401 MsG0480022003.01.T01:intron 15.0%
!! AAGAAGTTATATAATGAAAT+TGG - Chr4:66968607-66968626 MsG0480022003.01.T01:CDS 15.0%
!! AAGTTTAGTCATTATATAAA+GGG - Chr4:66968829-66968848 MsG0480022003.01.T01:CDS 15.0%
!! ATAGGTATAATAAAATTGTT+TGG + Chr4:66968316-66968335 None:intergenic 15.0%
!! ATTGGTTAATAAAAAATTCA+TGG - Chr4:66970232-66970251 MsG0480022003.01.T01:CDS 15.0%
!! TAGGTATAATAAAATTGTTT+GGG + Chr4:66968315-66968334 None:intergenic 15.0%
!! TTCAAATTTATTGCAAAAAA+AGG - Chr4:66969919-66969938 MsG0480022003.01.T01:intron 15.0%
!! TTGGTTAATAAAAAATTCAT+GGG - Chr4:66970233-66970252 MsG0480022003.01.T01:CDS 15.0%
!! AAATTTATTGCAAAAAAAGG+AGG - Chr4:66969922-66969941 MsG0480022003.01.T01:CDS 20.0%
!! AGGTATAATAAAATTGTTTG+GGG + Chr4:66968314-66968333 None:intergenic 20.0%
!! CAAGTTTAGTCATTATATAA+AGG - Chr4:66968828-66968847 MsG0480022003.01.T01:CDS 20.0%
!! GACAGTAAAATATGATTAAA+TGG + Chr4:66969636-66969655 None:intergenic 20.0%
!! TAAGACCAATTTGAAAAAAA+TGG - Chr4:66969541-66969560 MsG0480022003.01.T01:intron 20.0%
!! TAATATGAACTTGTGAAAAT+AGG - Chr4:66969748-66969767 MsG0480022003.01.T01:intron 20.0%
!! TACAAAAAAAAAAAAGCTTC+CGG + Chr4:66969266-66969285 None:intergenic 20.0%
!! TGGTTAATAAAAAATTCATG+GGG - Chr4:66970234-66970253 MsG0480022003.01.T01:CDS 20.0%
!!! AAGGAAAGCATTATTAAAAA+AGG - Chr4:66968897-66968916 MsG0480022003.01.T01:intron 20.0%
!!! AGACTAAAAACACATTTTTT+TGG - Chr4:66969054-66969073 MsG0480022003.01.T01:intron 20.0%
!!! AGTCATTACTTTGATTTATA+GGG - Chr4:66969020-66969039 MsG0480022003.01.T01:intron 20.0%
!!! CTTAAATGTCACAATTTTTT+AGG - Chr4:66969148-66969167 MsG0480022003.01.T01:intron 20.0%
!!! TAGTCATTACTTTGATTTAT+AGG - Chr4:66969019-66969038 MsG0480022003.01.T01:intron 20.0%
!!! TTAATAATGCTTTCCTTTTA+TGG + Chr4:66968894-66968913 None:intergenic 20.0%
!!! TTTTCAAATTGGTCTTAAAT+CGG + Chr4:66969538-66969557 None:intergenic 20.0%
!!! TTTTTTTTTTTTGTAGTGAC+CGG - Chr4:66969271-66969290 MsG0480022003.01.T01:intron 20.0%
! ACAAAAAAAAAAAAGCTTCC+GGG + Chr4:66969265-66969284 None:intergenic 25.0%
! ACCAATTTGAAAAAAATGGT+CGG - Chr4:66969545-66969564 MsG0480022003.01.T01:intron 25.0%
! CCAAACTAATGAAGAATTTA+AGG - Chr4:66968567-66968586 MsG0480022003.01.T01:CDS 25.0%
! CCTTAAATTCTTCATTAGTT+TGG + Chr4:66968570-66968589 None:intergenic 25.0%
! GATCGAAAAAATATGTCAAA+TGG - Chr4:66969424-66969443 MsG0480022003.01.T01:intron 25.0%
! TAGCATAGAATCTAATAAGT+AGG - Chr4:66969588-66969607 MsG0480022003.01.T01:intron 25.0%
! TCAAAGTAATGACTAAAATC+CGG + Chr4:66969014-66969033 None:intergenic 25.0%
! TCAAATATTCAAGAACAATG+TGG - Chr4:66968477-66968496 MsG0480022003.01.T01:CDS 25.0%
! TGTCAAATAAACATGAGAAA+TGG - Chr4:66968401-66968420 MsG0480022003.01.T01:CDS 25.0%
!! AAATTCTTCATTAGTTTGGT+CGG + Chr4:66968566-66968585 None:intergenic 25.0%
!! AGAAACACTCATTCTAAAAA+AGG - Chr4:66970294-66970313 MsG0480022003.01.T01:CDS 25.0%
!! CATTCTAAAAAAGGTTTATG+TGG - Chr4:66970303-66970322 MsG0480022003.01.T01:CDS 25.0%
!! TAGAAGATCATTTTCACTAT+TGG + Chr4:66970055-66970074 None:intergenic 25.0%
!!! TTAGCTTTTTTTTTTGGTAG+TGG - Chr4:66969226-66969245 MsG0480022003.01.T01:intron 25.0%
!!! TTTTTTTTTTTGTAGTGACC+GGG - Chr4:66969272-66969291 MsG0480022003.01.T01:intron 25.0%
AAAGTAATGACTAAAATCCG+GGG + Chr4:66969012-66969031 None:intergenic 30.0%
ATAAAAAATTCATGGGGTAC+TGG - Chr4:66970240-66970259 MsG0480022003.01.T01:CDS 30.0%
ATATAAAGGGTCATGCTAAT+CGG - Chr4:66968842-66968861 MsG0480022003.01.T01:CDS 30.0%
ATGGTTAATACTAAGGTTGA+AGG + Chr4:66968539-66968558 None:intergenic 30.0%
CAAAAAAAAAAAAGCTTCCG+GGG + Chr4:66969264-66969283 None:intergenic 30.0%
CAAAGTAATGACTAAAATCC+GGG + Chr4:66969013-66969032 None:intergenic 30.0%
CACATGATTATACTGAGTAT+TGG - Chr4:66970214-66970233 MsG0480022003.01.T01:CDS 30.0%
GTTGTGAAAATGAGAAACAT+AGG + Chr4:66968334-66968353 None:intergenic 30.0%
TCATGTTTATTTGACAAGCA+GGG + Chr4:66968397-66968416 None:intergenic 30.0%
TCTTAAATCGGTCTCTAATT+CGG + Chr4:66969526-66969545 None:intergenic 30.0%
TGGTTAATACTAAGGTTGAA+GGG + Chr4:66968538-66968557 None:intergenic 30.0%
TGTGAAAATAGGTAGTTGTT+GGG - Chr4:66969759-66969778 MsG0480022003.01.T01:intron 30.0%
TTGTGAAAATAGGTAGTTGT+TGG - Chr4:66969758-66969777 MsG0480022003.01.T01:intron 30.0%
! AAATGATCTTCTAAAAGCTG+TGG - Chr4:66970062-66970081 MsG0480022003.01.T01:CDS 30.0%
! ACACACTTGCACAAATATTT+TGG - Chr4:66969703-66969722 MsG0480022003.01.T01:intron 30.0%
! GAATTTAAGGCTTCACTTAA+TGG - Chr4:66968580-66968599 MsG0480022003.01.T01:CDS 30.0%
! GGAATTTTTACAGGAAAATG+TGG - Chr4:66970147-66970166 MsG0480022003.01.T01:CDS 30.0%
! TAAAAAATTCATGGGGTACT+GGG - Chr4:66970241-66970260 MsG0480022003.01.T01:CDS 30.0%
! TATTCAGGTGGAATTTTTAC+AGG - Chr4:66970138-66970157 MsG0480022003.01.T01:CDS 30.0%
! TATTTGGTGGATACTTTCTA+TGG + Chr4:66969803-66969822 None:intergenic 30.0%
! TGTTTCTCATTTTCACAACA+TGG - Chr4:66968335-66968354 MsG0480022003.01.T01:CDS 30.0%
!!! ACCGACCATTTTTTTCAAAT+TGG + Chr4:66969549-66969568 None:intergenic 30.0%
!!! TCTGGATTTCAGTTTTATTC+AGG - Chr4:66970123-66970142 MsG0480022003.01.T01:CDS 30.0%
!!! TTTTTTTTTTGTAGTGACCG+GGG - Chr4:66969273-66969292 MsG0480022003.01.T01:intron 30.0%
AACCTATGTTCCAACTAACA+TGG - Chr4:66968669-66968688 MsG0480022003.01.T01:CDS 35.0%
AAGCCTGTAACTGAGATTAA+AGG - Chr4:66970012-66970031 MsG0480022003.01.T01:CDS 35.0%
AGCCTGTAACTGAGATTAAA+GGG - Chr4:66970013-66970032 MsG0480022003.01.T01:CDS 35.0%
AGTATCCACCAAATAACAAC+AGG - Chr4:66969808-66969827 MsG0480022003.01.T01:intron 35.0%
ATCAAAAACCAAGGATCATG+TGG - Chr4:66968721-66968740 MsG0480022003.01.T01:CDS 35.0%
ATCCATGTTAGTTGGAACAT+AGG + Chr4:66968674-66968693 None:intergenic 35.0%
ATGTTCCAACTAACATGGAT+TGG - Chr4:66968674-66968693 MsG0480022003.01.T01:CDS 35.0%
ATTGAATCGTTCAATGCTGT+TGG - Chr4:66968505-66968524 MsG0480022003.01.T01:CDS 35.0%
CGGCAAAATGGTTAATACTA+AGG + Chr4:66968546-66968565 None:intergenic 35.0%
CTAGTTAAGCACACCATAAA+AGG - Chr4:66968878-66968897 MsG0480022003.01.T01:intron 35.0%
CTCATGTTTATTTGACAAGC+AGG + Chr4:66968398-66968417 None:intergenic 35.0%
GCATCAACATAAACTGATAC+AGG + Chr4:66970096-66970115 None:intergenic 35.0%
GTATCAGTTTATGTTGATGC+AGG - Chr4:66970096-66970115 MsG0480022003.01.T01:CDS 35.0%
TACAGATCATGTTGTTACTG+TGG - Chr4:66970176-66970195 MsG0480022003.01.T01:CDS 35.0%
TACCCTTTAATCTCAGTTAC+AGG + Chr4:66970018-66970037 None:intergenic 35.0%
TGCATAATATATGCAACGTC+CGG + Chr4:66969307-66969326 None:intergenic 35.0%
! AAAACGTTTTTCCTTCTCTG+CGG + Chr4:66968458-66968477 None:intergenic 35.0%
! AGATCATTTTCACTATTGGC+AGG + Chr4:66970051-66970070 None:intergenic 35.0%
! CTAGTTCACCTGTTGTTATT+TGG + Chr4:66969819-66969838 None:intergenic 35.0%
! TCTCGTCTGTTTCTTTGTAA+GGG + Chr4:66969969-66969988 None:intergenic 35.0%
!! CATGATCCTTGGTTTTTGAT+AGG + Chr4:66968721-66968740 None:intergenic 35.0%
!!! CTTTTTTTTTTGGTAGTGGC+CGG - Chr4:66969230-66969249 MsG0480022003.01.T01:intron 35.0%
!!! GGATTTCAGTTTTATTCAGG+TGG - Chr4:66970126-66970145 MsG0480022003.01.T01:CDS 35.0%
!!! TTTTTTTTTTGGTAGTGGCC+GGG - Chr4:66969231-66969250 MsG0480022003.01.T01:intron 35.0%
CATAATATATGCAACGTCCG+GGG + Chr4:66969305-66969324 None:intergenic 40.0%
CGAGACATGCAAAGTTAAGA+AGG - Chr4:66969984-66970003 MsG0480022003.01.T01:CDS 40.0%
CTTAGTGACTTTCGATCAGT+TGG - Chr4:66969469-66969488 MsG0480022003.01.T01:intron 40.0%
GATCATGTTGTTACTGTGGT+TGG - Chr4:66970180-66970199 MsG0480022003.01.T01:CDS 40.0%
GATCGAAAGTCACTAAGTCA+TGG + Chr4:66969466-66969485 None:intergenic 40.0%
GCATAATATATGCAACGTCC+GGG + Chr4:66969306-66969325 None:intergenic 40.0%
GTCACTCCTATCAAAAACCA+AGG - Chr4:66968712-66968731 MsG0480022003.01.T01:CDS 40.0%
TATGTTGATGCAGGAAGTTC+TGG - Chr4:66970105-66970124 MsG0480022003.01.T01:CDS 40.0%
TTATAAAGATGCCGCAGAGA+AGG - Chr4:66968444-66968463 MsG0480022003.01.T01:CDS 40.0%
TTCCTCCAATCCATGTTAGT+TGG + Chr4:66968682-66968701 None:intergenic 40.0%
! CATTAGTTTGGTCGGCAAAA+TGG + Chr4:66968558-66968577 None:intergenic 40.0%
! GTCTCGTCTGTTTCTTTGTA+AGG + Chr4:66969970-66969989 None:intergenic 40.0%
! GTTGTTACTGTGGTTGGTTA+TGG - Chr4:66970186-66970205 MsG0480022003.01.T01:CDS 40.0%
! TGAGCTTAGTTCAGTTGGTA+TGG + Chr4:66969334-66969353 None:intergenic 40.0%
! TTGTTGGGCATTTTCAACTG+TGG - Chr4:66969774-66969793 MsG0480022003.01.T01:intron 40.0%
!! AACATGGATTGGAGGAAAAG+AGG - Chr4:66968685-66968704 MsG0480022003.01.T01:CDS 40.0%
!! GTTCACCTGTTGTTATTTGG+TGG + Chr4:66969816-66969835 None:intergenic 40.0%
!! TTCCAACTAACATGGATTGG+AGG - Chr4:66968677-66968696 MsG0480022003.01.T01:CDS 40.0%
!!! TTTTTTTTTGGTAGTGGCCG+GGG - Chr4:66969232-66969251 MsG0480022003.01.T01:intron 40.0%
AACGGGATAAGAAGGATTGG+TGG + Chr4:66970334-66970353 None:intergenic 45.0%
ACCCTGCTAGACATTACATG+AGG + Chr4:66968367-66968386 None:intergenic 45.0%
AGCAACGGGATAAGAAGGAT+TGG + Chr4:66970337-66970356 None:intergenic 45.0%
CCCTCATGTAATGTCTAGCA+GGG - Chr4:66968363-66968382 MsG0480022003.01.T01:CDS 45.0%
CCCTGCTAGACATTACATGA+GGG + Chr4:66968366-66968385 None:intergenic 45.0%
GAACTGGTAGATTGTGTGAG+AGG - Chr4:66969856-66969875 MsG0480022003.01.T01:intron 45.0%
GTCGCTATCAGTACAAGAAC+TGG - Chr4:66969840-66969859 MsG0480022003.01.T01:intron 45.0%
TCCCTCATGTAATGTCTAGC+AGG - Chr4:66968362-66968381 MsG0480022003.01.T01:CDS 45.0%
TGTACGTACCACATGATCCT+TGG + Chr4:66968732-66968751 None:intergenic 45.0%
TGTGTGAGAGGTAATAGCAG+TGG - Chr4:66969868-66969887 MsG0480022003.01.T01:intron 45.0%
!! AAAATTCATGGGGTACTGGG+TGG - Chr4:66970244-66970263 MsG0480022003.01.T01:CDS 45.0%
!! AAATTCATGGGGTACTGGGT+GGG - Chr4:66970245-66970264 MsG0480022003.01.T01:CDS 45.0%
AATAGCAGTGGATGCAGTGG+TGG - Chr4:66969880-66969899 MsG0480022003.01.T01:intron 50.0%
ATGACTAAAATCCGGGGCAC+TGG + Chr4:66969006-66969025 None:intergenic 50.0%
CCAACTGAACTAAGCTCACG+AGG - Chr4:66969336-66969355 MsG0480022003.01.T01:intron 50.0%
CCTCGTGAGCTTAGTTCAGT+TGG + Chr4:66969339-66969358 None:intergenic 50.0%
GGTAATAGCAGTGGATGCAG+TGG - Chr4:66969877-66969896 MsG0480022003.01.T01:intron 50.0%
!! AAAATCCGGGGCACTGGTTA+AGG + Chr4:66969000-66969019 None:intergenic 50.0%
!! AAATCCGGGGCACTGGTTAA+GGG + Chr4:66968999-66969018 None:intergenic 50.0%
!! AATTCATGGGGTACTGGGTG+GGG - Chr4:66970246-66970265 MsG0480022003.01.T01:CDS 50.0%
AGTACCCTTAACCAGTGCCC+CGG - Chr4:66968992-66969011 MsG0480022003.01.T01:intron 55.0%
GGTGTGCTTAACTAGTGCCC+CGG + Chr4:66968873-66968892 None:intergenic 55.0%
GTACTGGGTGGGGTGAGAAA+GGG - Chr4:66970256-66970275 MsG0480022003.01.T01:CDS 55.0%
GTGTGCTTAACTAGTGCCCC+GGG + Chr4:66968872-66968891 None:intergenic 55.0%
TGTGCTTAACTAGTGCCCCG+GGG + Chr4:66968871-66968890 None:intergenic 55.0%
GTGCTTAACTAGTGCCCCGG+GGG + Chr4:66968870-66968889 None:intergenic 60.0%
! GGTACTGGGTGGGGTGAGAA+AGG - Chr4:66970255-66970274 MsG0480022003.01.T01:CDS 60.0%
! GTCATGCTAATCGGTGCCCC+CGG - Chr4:66968851-66968870 MsG0480022003.01.T01:CDS 60.0%
! TCATGCTAATCGGTGCCCCC+GGG - Chr4:66968852-66968871 MsG0480022003.01.T01:CDS 60.0%
AACGTCCGGGGTTCGAACCC+CGG + Chr4:66969293-66969312 None:intergenic 65.0%
AGCTTCCGGGGTTCGAACCC+CGG + Chr4:66969252-66969271 None:intergenic 65.0%
AGTGACCGGGGTTCGAACCC+CGG - Chr4:66969285-66969304 MsG0480022003.01.T01:intron 65.0%
! CATGCTAATCGGTGCCCCCG+GGG - Chr4:66968853-66968872 MsG0480022003.01.T01:CDS 65.0%
AGTGGCCGGGGTTCGAACCC+CGG - Chr4:66969244-66969263 MsG0480022003.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 66968304 66970359 66968304 ID=MsG0480022003.01;Name=MsG0480022003.01
Chr4 mRNA 66968304 66970359 66968304 ID=MsG0480022003.01.T01;Parent=MsG0480022003.01;Name=MsG0480022003.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|342
Chr4 exon 66969921 66970359 66969921 ID=MsG0480022003.01.T01:exon:7446;Parent=MsG0480022003.01.T01
Chr4 exon 66968304 66968893 66968304 ID=MsG0480022003.01.T01:exon:7445;Parent=MsG0480022003.01.T01
Chr4 CDS 66969921 66970359 66969921 ID=MsG0480022003.01.T01:cds;Parent=MsG0480022003.01.T01
Chr4 CDS 66968304 66968893 66968304 ID=MsG0480022003.01.T01:cds;Parent=MsG0480022003.01.T01
Gene Sequence

>MsG0480022003.01.T01

ATGACTTCCCCAAACAATTTTATTATACCTATGTTTCTCATTTTCACAACATGGATGCTCCCTCATGTAATGTCTAGCAGGGTTCTTGAACCCTGCTTGTCAAATAAACATGAGAAATGGATGACACAATTTGCAAAATCTTATAAAGATGCCGCAGAGAAGGAAAAACGTTTTCAAATATTCAAGAACAATGTGGAGTTCATTGAATCGTTCAATGCTGTTGGAGACAAACCCTTCAACCTTAGTATTAACCATTTTGCCGACCAAACTAATGAAGAATTTAAGGCTTCACTTAATGGCAAGAAGAAGTTATATAATGAAATTGGCACTTTAAATGAAACTACATCGTTCAAGTATCACAATGTAACCTATGTTCCAACTAACATGGATTGGAGGAAAAGAGGTGCTGTCACTCCTATCAAAAACCAAGGATCATGTGGTAGTTGTTGGGCATTTTCAACTGTGGCTTCCATAGAAAGTATCCACCAAATAACAACAGGTGAACTAGTGTCGCTATCAGTACAAGAACTGGTAGATTGTGTGAGAGGTAATAGCAGTGGATGCAGTGGTGGTTACTTAGAAGATGCATTCAAATTTATTGCAAAAAAAGGAGGAATAGCAAGTGAAGCAAACTACCCTTACAAAGAAACAGACGAGACATGCAAAGTTAAGAAGGAAGCAAAGCCTGTAACTGAGATTAAAGGGTACGAGAAAGTTCCTGCCAATAGTGAAAATGATCTTCTAAAAGCTGTGGCAAATCAACCTGTATCAGTTTATGTTGATGCAGGAAGTTCTGGATTTCAGTTTTATTCAGGTGGAATTTTTACAGGAAAATGTGGAACTGATACAGATCATGTTGTTACTGTGGTTGGTTATGGTGTATCACATGATTATACTGAGTATTGGTTAATAAAAAATTCATGGGGTACTGGGTGGGGTGAGAAAGGGTACTTGAAATTGAAGAGAAACACTCATTCTAAAAAAGGTTTATGTGGAATTGCCACCAATCCTTCTTATCCCGTTGCTTAA
Protein sequence

>MsG0480022003.01.T01

MTSPNNFIIPMFLIFTTWMLPHVMSSRVLEPCLSNKHEKWMTQFAKSYKDAAEKEKRFQIFKNNVEFIESFNAVGDKPFNLSINHFADQTNEEFKASLNGKKKLYNEIGTLNETTSFKYHNVTYVPTNMDWRKRGAVTPIKNQGSCGSCWAFSTVASIESIHQITTGELVSLSVQELVDCVRGNSSGCSGGYLEDAFKFIAKKGGIASEANYPYKETDETCKVKKEAKPVTEIKGYEKVPANSENDLLKAVANQPVSVYVDAGSSGFQFYSGGIFTGKCGTDTDHVVTVVGYGVSHDYTEYWLIKNSWGTGWGEKGYLKLKRNTHSKKGLCGIATNPSYPVA*