Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024975.01.T01 | XP_003612031.3 | 91.477 | 176 | 14 | 1 | 1 | 175 | 301 | 476 | 8.36E-108 | 335 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024975.01.T01 | O48700 | 25.532 | 188 | 105 | 5 | 1 | 175 | 303 | 468 | 4.99E-13 | 69.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000097.01 | MsG0580024975.01 | -0.850449 | 1.685480e-60 | 3.302911e-57 |
| MsG0180000151.01 | MsG0580024975.01 | 0.847980 | 8.200468e-60 | 1.482849e-56 |
| MsG0180000221.01 | MsG0580024975.01 | 0.850086 | 2.131141e-60 | 4.126918e-57 |
| MsG0180001119.01 | MsG0580024975.01 | 0.840031 | 1.110877e-57 | 1.562736e-54 |
| MsG0180001120.01 | MsG0580024975.01 | 0.851534 | 8.334774e-61 | 1.692188e-57 |
| MsG0180001122.01 | MsG0580024975.01 | 0.834609 | 2.714827e-56 | 3.238312e-53 |
| MsG0180001435.01 | MsG0580024975.01 | 0.806036 | 9.997896e-50 | 5.371305e-47 |
| MsG0180001590.01 | MsG0580024975.01 | 0.828572 | 8.344749e-55 | 8.326860e-52 |
| MsG0180001591.01 | MsG0580024975.01 | 0.808487 | 3.022932e-50 | 1.730688e-47 |
| MsG0180002085.01 | MsG0580024975.01 | 0.811364 | 7.264951e-51 | 4.488130e-48 |
| MsG0180002088.01 | MsG0580024975.01 | 0.861090 | 1.309666e-63 | 3.661344e-60 |
| MsG0180004106.01 | MsG0580024975.01 | 0.804301 | 2.306671e-49 | 1.185160e-46 |
| MsG0180004112.01 | MsG0580024975.01 | 0.825237 | 5.228830e-54 | 4.738958e-51 |
| MsG0180004167.01 | MsG0580024975.01 | 0.830149 | 3.454490e-55 | 3.609468e-52 |
| MsG0180005639.01 | MsG0580024975.01 | 0.823417 | 1.400955e-53 | 1.205442e-50 |
| MsG0180005772.01 | MsG0580024975.01 | -0.809125 | 2.208392e-50 | 1.285859e-47 |
| MsG0180006054.01 | MsG0580024975.01 | 0.816009 | 6.897784e-52 | 4.830658e-49 |
| MsG0280011272.01 | MsG0580024975.01 | -0.810313 | 1.226338e-50 | 7.367816e-48 |
| MsG0280011309.01 | MsG0580024975.01 | 0.820413 | 6.951780e-53 | 5.497011e-50 |
| MsG0380012085.01 | MsG0580024975.01 | 0.837341 | 5.504136e-57 | 7.127955e-54 |
| MsG0380012091.01 | MsG0580024975.01 | 0.828283 | 9.796090e-55 | 9.692898e-52 |
| MsG0380012249.01 | MsG0580024975.01 | 0.864241 | 1.400693e-64 | 4.367825e-61 |
| MsG0380012319.01 | MsG0580024975.01 | 0.838562 | 2.671245e-57 | 3.591425e-54 |
| MsG0380012321.01 | MsG0580024975.01 | 0.821036 | 4.999246e-53 | 4.022279e-50 |
| MsG0380012331.01 | MsG0580024975.01 | 0.842819 | 2.048758e-58 | 3.143406e-55 |
| MsG0380012340.01 | MsG0580024975.01 | 0.820902 | 5.366590e-53 | 4.301555e-50 |
| MsG0380012353.01 | MsG0580024975.01 | 0.806834 | 6.785678e-50 | 3.720741e-47 |
| MsG0380012356.01 | MsG0580024975.01 | 0.830787 | 2.412111e-55 | 2.567606e-52 |
| MsG0380012357.01 | MsG0580024975.01 | 0.887902 | 9.481449e-73 | 7.184936e-69 |
| MsG0380013474.01 | MsG0580024975.01 | 0.804129 | 2.504805e-49 | 1.281270e-46 |
| MsG0380014333.01 | MsG0580024975.01 | 0.869319 | 3.385161e-66 | 1.265028e-62 |
| MsG0380014727.01 | MsG0580024975.01 | 0.803644 | 3.159766e-49 | 1.596256e-46 |
| MsG0480020433.01 | MsG0580024975.01 | 0.845267 | 4.518492e-59 | 7.489059e-56 |
| MsG0480021763.01 | MsG0580024975.01 | 0.815123 | 1.086054e-51 | 7.423159e-49 |
| MsG0480022003.01 | MsG0580024975.01 | 0.809418 | 1.911276e-50 | 1.121601e-47 |
| MsG0480022066.01 | MsG0580024975.01 | 0.839294 | 1.726926e-57 | 2.374197e-54 |
| MsG0480022330.01 | MsG0580024975.01 | 0.808738 | 2.671865e-50 | 1.539626e-47 |
| MsG0480022535.01 | MsG0580024975.01 | 0.868107 | 8.345508e-66 | 2.983418e-62 |
| MsG0480022572.01 | MsG0580024975.01 | 0.819727 | 9.978359e-53 | 7.740965e-50 |
| MsG0480022802.01 | MsG0580024975.01 | 0.808561 | 2.915759e-50 | 1.672494e-47 |
| MsG0480023251.01 | MsG0580024975.01 | 0.834144 | 3.551164e-56 | 4.176672e-53 |
| MsG0480023491.01 | MsG0580024975.01 | 0.839531 | 1.499229e-57 | 2.076171e-54 |
| MsG0580024975.01 | MsG0580025644.01 | 0.804372 | 2.229939e-49 | 1.147813e-46 |
| MsG0580024975.01 | MsG0580025850.01 | 0.802210 | 6.249089e-49 | 3.043365e-46 |
| MsG0580024975.01 | MsG0580027789.01 | 0.827571 | 1.453288e-54 | 1.408317e-51 |
| MsG0580024975.01 | MsG0580027923.01 | 0.833850 | 4.208745e-56 | 4.907510e-53 |
| MsG0580024975.01 | MsG0580029195.01 | 0.818259 | 2.152415e-52 | 1.603120e-49 |
| MsG0580024975.01 | MsG0580029346.01 | 0.809528 | 1.809990e-50 | 1.065248e-47 |
| MsG0580024975.01 | MsG0580029880.01 | 0.802224 | 6.210052e-49 | 3.025389e-46 |
| MsG0580024975.01 | MsG0680030558.01 | 0.833297 | 5.783395e-56 | 6.632250e-53 |
| MsG0580024975.01 | MsG0680030994.01 | 0.858869 | 6.127262e-63 | 1.586898e-59 |
| MsG0580024975.01 | MsG0680031424.01 | 0.824460 | 7.974572e-54 | 7.067568e-51 |
| MsG0580024975.01 | MsG0680031996.01 | 0.836019 | 1.195787e-56 | 1.487965e-53 |
| MsG0580024975.01 | MsG0680031998.01 | 0.810724 | 9.996935e-51 | 6.072376e-48 |
| MsG0580024975.01 | MsG0680032279.01 | 0.820308 | 7.345653e-53 | 5.791276e-50 |
| MsG0580024975.01 | MsG0680032305.01 | 0.806373 | 8.488437e-50 | 4.599641e-47 |
| MsG0580024975.01 | MsG0680033465.01 | 0.808934 | 2.426230e-50 | 1.405386e-47 |
| MsG0580024975.01 | MsG0680033639.01 | 0.812306 | 4.530084e-51 | 2.870149e-48 |
| MsG0580024975.01 | MsG0680034028.01 | 0.803426 | 3.506635e-49 | 1.761616e-46 |
| MsG0580024975.01 | MsG0680034032.01 | 0.856318 | 3.488257e-62 | 8.292206e-59 |
| MsG0580024975.01 | MsG0680034769.01 | 0.838120 | 3.473045e-57 | 4.606519e-54 |
| MsG0580024975.01 | MsG0780036116.01 | 0.823985 | 1.031016e-53 | 9.015646e-51 |
| MsG0580024975.01 | MsG0780036126.01 | 0.809749 | 1.622554e-50 | 9.606067e-48 |
| MsG0580024975.01 | MsG0780036509.01 | 0.828309 | 9.658360e-55 | 9.563663e-52 |
| MsG0580024975.01 | MsG0780036626.01 | 0.805774 | 1.134608e-49 | 6.055051e-47 |
| MsG0580024975.01 | MsG0780037105.01 | 0.803699 | 3.078120e-49 | 1.557213e-46 |
| MsG0580024975.01 | MsG0780037679.01 | 0.805077 | 1.588537e-49 | 8.326619e-47 |
| MsG0580024975.01 | MsG0780039711.01 | 0.833934 | 4.009054e-56 | 4.686690e-53 |
| MsG0580024975.01 | MsG0780040285.01 | 0.809065 | 2.275131e-50 | 1.322556e-47 |
| MsG0580024975.01 | MsG0780041738.01 | 0.813970 | 1.954353e-51 | 1.294805e-48 |
| MsG0580024975.01 | MsG0780041742.01 | 0.803968 | 2.706457e-49 | 1.378728e-46 |
| MsG0580024975.01 | MsG0780041753.01 | 0.811934 | 5.462030e-51 | 3.425538e-48 |
| MsG0580024975.01 | MsG0880043167.01 | 0.834506 | 2.881903e-56 | 3.427213e-53 |
| MsG0580024975.01 | MsG0880044705.01 | 0.843989 | 9.980775e-59 | 1.588825e-55 |
| MsG0580024975.01 | MsG0880045370.01 | 0.820323 | 7.288201e-53 | 5.748181e-50 |
| MsG0580024975.01 | MsG0880045386.01 | 0.830277 | 3.214912e-55 | 3.371361e-52 |
| MsG0580024975.01 | MsG0880045624.01 | 0.823280 | 1.507845e-53 | 1.292510e-50 |
| MsG0580024975.01 | MsG0880045690.01 | 0.807961 | 3.913092e-50 | 2.209471e-47 |
| MsG0580024975.01 | MsG0880045771.01 | 0.808784 | 2.612895e-50 | 1.507469e-47 |
| MsG0580024975.01 | MsG0880046296.01 | 0.851412 | 9.026489e-61 | 1.825631e-57 |
| MsG0380015049.01 | MsG0580024975.01 | 0.817550 | 3.111094e-52 | 2.272637e-49 |
| MsG0380015640.01 | MsG0580024975.01 | 0.807013 | 6.219454e-50 | 3.426022e-47 |
| MsG0380015735.01 | MsG0580024975.01 | 0.899925 | 1.222626e-77 | 1.544418e-73 |
| MsG0380015804.01 | MsG0580024975.01 | 0.809298 | 2.028116e-50 | 1.186338e-47 |
| MsG0380015810.01 | MsG0580024975.01 | 0.840972 | 6.301296e-58 | 9.125271e-55 |
| MsG0380016201.01 | MsG0580024975.01 | -0.815960 | 7.074149e-52 | 4.947463e-49 |
| MsG0380017220.01 | MsG0580024975.01 | 0.816235 | 6.138485e-52 | 4.325604e-49 |
| MsG0480018253.01 | MsG0580024975.01 | 0.813407 | 2.599790e-51 | 1.696402e-48 |
| MsG0280007339.01 | MsG0580024975.01 | 0.814657 | 1.377818e-51 | 9.299768e-49 |
| MsG0280008165.01 | MsG0580024975.01 | 0.805002 | 1.646771e-49 | 8.615344e-47 |
| MsG0280008171.01 | MsG0580024975.01 | 0.828293 | 9.744845e-55 | 9.644706e-52 |
| MsG0280008172.01 | MsG0580024975.01 | 0.846938 | 1.585568e-59 | 2.773316e-56 |
| MsG0280009551.01 | MsG0580024975.01 | 0.855608 | 5.627213e-62 | 1.306486e-58 |
| MsG0280009624.01 | MsG0580024975.01 | 0.805502 | 1.294431e-49 | 6.859287e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024975.01.T01 | MTR_5g020570 | 91.477 | 176 | 14 | 1 | 1 | 175 | 301 | 476 | 1.01e-111 | 335 |
| MsG0580024975.01.T01 | MTR_5g020570 | 91.477 | 176 | 14 | 1 | 1 | 175 | 316 | 491 | 2.55e-111 | 334 |
| MsG0580024975.01.T01 | MTR_8g077205 | 50.847 | 177 | 84 | 2 | 1 | 176 | 191 | 365 | 1.43e-57 | 191 |
| MsG0580024975.01.T01 | MTR_8g077205 | 50.847 | 177 | 84 | 2 | 1 | 176 | 303 | 477 | 5.72e-57 | 191 |
| MsG0580024975.01.T01 | MTR_7g116600 | 40.984 | 61 | 36 | 0 | 2 | 62 | 107 | 167 | 7.45e-14 | 68.9 |
| MsG0580024975.01.T01 | MTR_3g085610 | 25.714 | 175 | 113 | 3 | 3 | 175 | 310 | 469 | 4.38e-13 | 67.0 |
| MsG0580024975.01.T01 | MTR_8g080280 | 48.936 | 47 | 24 | 0 | 3 | 49 | 312 | 358 | 3.88e-12 | 63.9 |
| MsG0580024975.01.T01 | MTR_1g093965 | 52.174 | 46 | 22 | 0 | 3 | 48 | 18 | 63 | 3.16e-11 | 59.7 |
| MsG0580024975.01.T01 | MTR_4g485520 | 46.809 | 47 | 25 | 0 | 2 | 48 | 302 | 348 | 5.20e-11 | 60.8 |
| MsG0580024975.01.T01 | MTR_4g485520 | 46.809 | 47 | 25 | 0 | 2 | 48 | 134 | 180 | 6.50e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024975.01.T01 | AT1G24330 | 25.532 | 188 | 105 | 5 | 1 | 175 | 303 | 468 | 5.08e-14 | 69.7 |
| MsG0580024975.01.T01 | AT1G24330 | 25.532 | 188 | 105 | 5 | 1 | 175 | 303 | 468 | 5.08e-14 | 69.7 |
| MsG0580024975.01.T01 | AT4G36550 | 30.682 | 176 | 108 | 7 | 1 | 170 | 247 | 414 | 1.06e-13 | 68.6 |
| MsG0580024975.01.T01 | AT1G67530 | 45.455 | 55 | 30 | 0 | 3 | 57 | 302 | 356 | 9.30e-13 | 65.9 |
| MsG0580024975.01.T01 | AT3G07360 | 51.111 | 45 | 22 | 0 | 1 | 45 | 102 | 146 | 1.09e-12 | 65.5 |
| MsG0580024975.01.T01 | AT1G67530 | 43.860 | 57 | 32 | 0 | 1 | 57 | 300 | 356 | 1.11e-12 | 65.9 |
| MsG0580024975.01.T01 | AT1G67530 | 43.860 | 57 | 32 | 0 | 1 | 57 | 300 | 356 | 1.11e-12 | 65.9 |
| MsG0580024975.01.T01 | AT1G67530 | 43.860 | 57 | 32 | 0 | 1 | 57 | 300 | 356 | 1.11e-12 | 65.9 |
| MsG0580024975.01.T01 | AT2G23140 | 47.170 | 53 | 28 | 0 | 2 | 54 | 70 | 122 | 1.15e-12 | 65.5 |
| MsG0580024975.01.T01 | AT2G23140 | 47.170 | 53 | 28 | 0 | 2 | 54 | 70 | 122 | 1.15e-12 | 65.5 |
| MsG0580024975.01.T01 | AT2G23140 | 47.170 | 53 | 28 | 0 | 2 | 54 | 259 | 311 | 1.37e-12 | 65.5 |
| MsG0580024975.01.T01 | AT2G23140 | 47.170 | 53 | 28 | 0 | 2 | 54 | 259 | 311 | 1.37e-12 | 65.5 |
| MsG0580024975.01.T01 | AT2G23140 | 47.170 | 53 | 28 | 0 | 2 | 54 | 262 | 314 | 1.37e-12 | 65.5 |
| MsG0580024975.01.T01 | AT1G27910 | 51.020 | 49 | 24 | 0 | 1 | 49 | 307 | 355 | 1.55e-12 | 65.5 |
| MsG0580024975.01.T01 | AT5G67340 | 41.791 | 67 | 36 | 1 | 1 | 64 | 268 | 334 | 5.65e-12 | 63.5 |
| MsG0580024975.01.T01 | AT5G67340 | 41.791 | 67 | 36 | 1 | 1 | 64 | 268 | 334 | 5.65e-12 | 63.5 |
| MsG0580024975.01.T01 | AT5G42340 | 46.809 | 47 | 25 | 0 | 3 | 49 | 320 | 366 | 6.93e-11 | 60.5 |
Find 52 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATTGGATGCTGCAATTT+TGG | 0.187482 | 5:-12421980 | None:intergenic |
| GGATTATCTTAAGTTGATTT+TGG | 0.208826 | 5:+12422338 | MsG0580024975.01.T01:three_prime_UTR |
| AAGTCCTTCATGACAATATT+TGG | 0.222378 | 5:-12420894 | None:intergenic |
| GCCATAATGGAAGAACTTTC+TGG | 0.238374 | 5:+12421941 | MsG0580024975.01.T01:three_prime_UTR |
| TCCAGAAAGTTCTTCCATTA+TGG | 0.272030 | 5:-12421942 | None:intergenic |
| AACAGCTCTGTCTTTGAAAA+AGG | 0.287943 | 5:-12422136 | None:intergenic |
| CAATCACCTCCATAAGAGTT+TGG | 0.288506 | 5:+12421000 | MsG0580024975.01.T01:CDS |
| GAAATAATCCCAAACTCTTA+TGG | 0.300242 | 5:-12421009 | None:intergenic |
| CAACATGTCACTTGGATCAT+TGG | 0.319176 | 5:+12421061 | MsG0580024975.01.T01:CDS |
| AATCACCTCCATAAGAGTTT+GGG | 0.356606 | 5:+12421001 | MsG0580024975.01.T01:CDS |
| CTCAACTTCATGATCTTCAA+TGG | 0.371229 | 5:+12421225 | MsG0580024975.01.T01:CDS |
| CTTCTTGATTCAGAAGTGAT+AGG | 0.387240 | 5:+12421908 | MsG0580024975.01.T01:intron |
| ACTTATGCATCCCTTTGTTT+CGG | 0.390748 | 5:-12422223 | None:intergenic |
| AGGAGAAGTACTTGCCATAA+TGG | 0.393561 | 5:+12421928 | MsG0580024975.01.T01:three_prime_UTR |
| TCACAGAATGGAAATGATAA+AGG | 0.407952 | 5:+12422393 | MsG0580024975.01.T01:three_prime_UTR |
| TCAACTTCATGATCTTCAAT+GGG | 0.469345 | 5:+12421226 | MsG0580024975.01.T01:CDS |
| TCTGTATGTCTACCTGGATC+AGG | 0.469637 | 5:-12420954 | None:intergenic |
| CATCAGATTCAAGTCATGAT+TGG | 0.470843 | 5:-12421120 | None:intergenic |
| TGTACATGTTTCTCAAAATG+TGG | 0.490678 | 5:+12422317 | MsG0580024975.01.T01:three_prime_UTR |
| AAAGAATGTGGATAAGAAAG+TGG | 0.491198 | 5:+12420808 | None:intergenic |
| AATTCTATTGCCGAAACAAA+GGG | 0.496586 | 5:+12422213 | MsG0580024975.01.T01:three_prime_UTR |
| ATGGAAGAACTTTCTGGAGA+TGG | 0.506876 | 5:+12421947 | MsG0580024975.01.T01:three_prime_UTR |
| GGTGTCTCCATTCCTGATCC+AGG | 0.507047 | 5:+12420942 | MsG0580024975.01.T01:CDS |
| TATTGCTGAAATACTTGAGA+TGG | 0.508170 | 5:+12422248 | MsG0580024975.01.T01:three_prime_UTR |
| TACTTGATTCTGATAACAAA+GGG | 0.514099 | 5:+12422022 | MsG0580024975.01.T01:three_prime_UTR |
| GACTCCAAATATTGTCATGA+AGG | 0.514758 | 5:+12420890 | MsG0580024975.01.T01:CDS |
| GATAAGAAAGTGGATTAATG+AGG | 0.523558 | 5:+12420818 | MsG0580024975.01.T01:CDS |
| TTTGGAGTCAATGTCATATG+AGG | 0.534604 | 5:-12420876 | None:intergenic |
| AAATCTTCTGTATGTCTACC+TGG | 0.534943 | 5:-12420960 | None:intergenic |
| AACATTTGTGATACTGAAGA+AGG | 0.537856 | 5:+12422186 | MsG0580024975.01.T01:three_prime_UTR |
| TGGACAAACTTCACTGTTGT+AGG | 0.541725 | 5:-12422079 | None:intergenic |
| GACATGTTGGTAAGATCCAT+TGG | 0.542509 | 5:-12421047 | None:intergenic |
| GCATGAACTGTATGCTTGTG+AGG | 0.545176 | 5:-12421170 | None:intergenic |
| CAATGATCTGAACTTGCCAA+TGG | 0.550541 | 5:+12421031 | MsG0580024975.01.T01:CDS |
| GATCTTACCAACATGTCACT+TGG | 0.560709 | 5:+12421053 | MsG0580024975.01.T01:CDS |
| TTCTGTGAGATATAGAAAAG+AGG | 0.564373 | 5:-12422378 | None:intergenic |
| TTACTTGATTCTGATAACAA+AGG | 0.566230 | 5:+12422021 | MsG0580024975.01.T01:three_prime_UTR |
| AAATTCTATTGCCGAAACAA+AGG | 0.571416 | 5:+12422212 | MsG0580024975.01.T01:three_prime_UTR |
| CAATGATCCAAGTGACATGT+TGG | 0.572790 | 5:-12421060 | None:intergenic |
| TAAGACAGAAGTGAGTGCAT+TGG | 0.577623 | 5:-12421995 | None:intergenic |
| ATGTCTACCTGGATCAGGAA+TGG | 0.578674 | 5:-12420949 | None:intergenic |
| CTGACACTGACAAAGTAGCT+TGG | 0.600806 | 5:-12421301 | None:intergenic |
| ATAAGAAAGTGGATTAATGA+GGG | 0.602588 | 5:+12420819 | MsG0580024975.01.T01:CDS |
| CAGTGAAGTTTGTCCACACA+TGG | 0.607997 | 5:+12422086 | MsG0580024975.01.T01:three_prime_UTR |
| GAATGGAAATGATAAAGGAA+AGG | 0.610121 | 5:+12422398 | MsG0580024975.01.T01:three_prime_UTR |
| CATTGAAGATCATGAAGTTG+AGG | 0.619411 | 5:-12421224 | None:intergenic |
| GGTCTTAACTTGTGACACAT+CGG | 0.622908 | 5:-12421099 | None:intergenic |
| ATAATCCCAAACTCTTATGG+AGG | 0.634502 | 5:-12421006 | None:intergenic |
| TCAAATATGAAGCAATTCCA+CGG | 0.635021 | 5:-12422427 | None:intergenic |
| TAAAGGAAAGGAAAGTGCCG+TGG | 0.657866 | 5:+12422410 | MsG0580024975.01.T01:three_prime_UTR |
| TGAAGGACTTGATATCAAAG+TGG | 0.687601 | 5:+12420907 | MsG0580024975.01.T01:CDS |
| GCATTGACAGATACCATGTG+TGG | 0.707970 | 5:-12422099 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACAATGAAAATAGTTTATA+TGG | - | Chr5:12421509-12421528 | None:intergenic | 15.0% |
| !! | TATAAATAATTAAGTTCCTA+AGG | + | Chr5:12421643-12421662 | MsG0580024975.01.T01:intron | 15.0% |
| !! | AAATTTATAATACCAAAAGC+AGG | - | Chr5:12421811-12421830 | None:intergenic | 20.0% |
| !! | TATATGTCCAAAAACAAAAA+AGG | + | Chr5:12420848-12420867 | MsG0580024975.01.T01:CDS | 20.0% |
| !!! | AACTATTTTCATTGTAAATG+TGG | + | Chr5:12421513-12421532 | MsG0580024975.01.T01:intron | 20.0% |
| !!! | TTTGAAAAAGGTAACATTTT+GGG | - | Chr5:12422127-12422146 | None:intergenic | 20.0% |
| ! | ACCATACAAAGATCAAAATT+GGG | - | Chr5:12421460-12421479 | None:intergenic | 25.0% |
| ! | TACTTGATTCTGATAACAAA+GGG | + | Chr5:12422022-12422041 | MsG0580024975.01.T01:three_prime_UTR | 25.0% |
| ! | TTACTTGATTCTGATAACAA+AGG | + | Chr5:12422021-12422040 | MsG0580024975.01.T01:three_prime_UTR | 25.0% |
| !! | TACAGAAGATTTTATGTTGT+TGG | + | Chr5:12420971-12420990 | MsG0580024975.01.T01:CDS | 25.0% |
| !! | TCTACAATTTCAGAAACTTT+TGG | - | Chr5:12421598-12421617 | None:intergenic | 25.0% |
| !!! | AAGTTTCTGAAATTGTAGAA+AGG | + | Chr5:12421599-12421618 | MsG0580024975.01.T01:intron | 25.0% |
| !!! | ATAAGAAAGTGGATTAATGA+GGG | + | Chr5:12420819-12420838 | MsG0580024975.01.T01:CDS | 25.0% |
| !!! | CTTTGAAAAAGGTAACATTT+TGG | - | Chr5:12422128-12422147 | None:intergenic | 25.0% |
| !!! | GATTATTTTATGCATGTTGT+TGG | - | Chr5:12422049-12422068 | None:intergenic | 25.0% |
| !!! | GGATTATCTTAAGTTGATTT+TGG | + | Chr5:12422338-12422357 | MsG0580024975.01.T01:three_prime_UTR | 25.0% |
| !!! | GTAATTTTTCAGAAATGGTA+TGG | + | Chr5:12421849-12421868 | MsG0580024975.01.T01:intron | 25.0% |
| !!! | TGTGTGTAATTTTTCAGAAA+TGG | + | Chr5:12421844-12421863 | MsG0580024975.01.T01:intron | 25.0% |
| AAATTCTATTGCCGAAACAA+AGG | + | Chr5:12422212-12422231 | MsG0580024975.01.T01:three_prime_UTR | 30.0% | |
| AACATTTGTGATACTGAAGA+AGG | + | Chr5:12422186-12422205 | MsG0580024975.01.T01:three_prime_UTR | 30.0% | |
| AAGTCCTTCATGACAATATT+TGG | - | Chr5:12420897-12420916 | None:intergenic | 30.0% | |
| AATTCTATTGCCGAAACAAA+GGG | + | Chr5:12422213-12422232 | MsG0580024975.01.T01:three_prime_UTR | 30.0% | |
| CAAGAAACTTAAAATTTGCG+TGG | - | Chr5:12421747-12421766 | None:intergenic | 30.0% | |
| CACCATACAAAGATCAAAAT+TGG | - | Chr5:12421461-12421480 | None:intergenic | 30.0% | |
| GAAATAATCCCAAACTCTTA+TGG | - | Chr5:12421012-12421031 | None:intergenic | 30.0% | |
| GAATGGAAATGATAAAGGAA+AGG | + | Chr5:12422398-12422417 | MsG0580024975.01.T01:three_prime_UTR | 30.0% | |
| TATTGCTGAAATACTTGAGA+TGG | + | Chr5:12422248-12422267 | MsG0580024975.01.T01:three_prime_UTR | 30.0% | |
| TCAAAGTGGTGCAAAAATAA+TGG | + | Chr5:12420921-12420940 | MsG0580024975.01.T01:CDS | 30.0% | |
| TCAAATATGAAGCAATTCCA+CGG | - | Chr5:12422430-12422449 | None:intergenic | 30.0% | |
| TCAACTTCATGATCTTCAAT+GGG | + | Chr5:12421226-12421245 | MsG0580024975.01.T01:CDS | 30.0% | |
| TCACAGAATGGAAATGATAA+AGG | + | Chr5:12422393-12422412 | MsG0580024975.01.T01:three_prime_UTR | 30.0% | |
| TGTACATGTTTCTCAAAATG+TGG | + | Chr5:12422317-12422336 | MsG0580024975.01.T01:three_prime_UTR | 30.0% | |
| TTATGAACGTATCTTCACTT+AGG | - | Chr5:12421878-12421897 | None:intergenic | 30.0% | |
| TTCTGTGAGATATAGAAAAG+AGG | - | Chr5:12422381-12422400 | None:intergenic | 30.0% | |
| ! | AACTTTTGGATGATTCACTT+AGG | - | Chr5:12421584-12421603 | None:intergenic | 30.0% |
| ! | AATCAAGCAAACGAATTTTC+AGG | - | Chr5:12421774-12421793 | None:intergenic | 30.0% |
| ! | CTTTTCTATATCTCACAGAA+TGG | + | Chr5:12422381-12422400 | MsG0580024975.01.T01:three_prime_UTR | 30.0% |
| !! | GATAAGAAAGTGGATTAATG+AGG | + | Chr5:12420818-12420837 | MsG0580024975.01.T01:CDS | 30.0% |
| !!! | GGTAGTTCCTTTTTTGTTTT+TGG | - | Chr5:12420858-12420877 | None:intergenic | 30.0% |
| AAAATTTGCGTGGATCAACT+TGG | - | Chr5:12421737-12421756 | None:intergenic | 35.0% | |
| AAATCTTCTGTATGTCTACC+TGG | - | Chr5:12420963-12420982 | None:intergenic | 35.0% | |
| AATCACCTCCATAAGAGTTT+GGG | + | Chr5:12421001-12421020 | MsG0580024975.01.T01:CDS | 35.0% | |
| ACAAAGATCAAAATTGGGCA+AGG | - | Chr5:12421455-12421474 | None:intergenic | 35.0% | |
| ATAATCCCAAACTCTTATGG+AGG | - | Chr5:12421009-12421028 | None:intergenic | 35.0% | |
| ATCCTCAATCATATCCATGT+CGG | - | Chr5:12421673-12421692 | None:intergenic | 35.0% | |
| CAAAGATCAAAATTGGGCAA+GGG | - | Chr5:12421454-12421473 | None:intergenic | 35.0% | |
| CATCAGATTCAAGTCATGAT+TGG | - | Chr5:12421123-12421142 | None:intergenic | 35.0% | |
| CATTGAAGATCATGAAGTTG+AGG | - | Chr5:12421227-12421246 | None:intergenic | 35.0% | |
| CTCAACTTCATGATCTTCAA+TGG | + | Chr5:12421225-12421244 | MsG0580024975.01.T01:CDS | 35.0% | |
| CTTCTTGATTCAGAAGTGAT+AGG | + | Chr5:12421908-12421927 | MsG0580024975.01.T01:intron | 35.0% | |
| GACTCCAAATATTGTCATGA+AGG | + | Chr5:12420890-12420909 | MsG0580024975.01.T01:CDS | 35.0% | |
| GTGAAGATACGTTCATAAGT+TGG | + | Chr5:12421880-12421899 | MsG0580024975.01.T01:intron | 35.0% | |
| TCCAGAAAGTTCTTCCATTA+TGG | - | Chr5:12421945-12421964 | None:intergenic | 35.0% | |
| TGAAGGACTTGATATCAAAG+TGG | + | Chr5:12420907-12420926 | MsG0580024975.01.T01:CDS | 35.0% | |
| TTGCTCAAAAATCTGACTAC+TGG | - | Chr5:12421343-12421362 | None:intergenic | 35.0% | |
| ! | AACAGCTCTGTCTTTGAAAA+AGG | - | Chr5:12422139-12422158 | None:intergenic | 35.0% |
| ! | ACTTATGCATCCCTTTGTTT+CGG | - | Chr5:12422226-12422245 | None:intergenic | 35.0% |
| ! | TTTATCTTTGCACCTGCTTT+TGG | + | Chr5:12421796-12421815 | MsG0580024975.01.T01:intron | 35.0% |
| ! | TTTGGAGTCAATGTCATATG+AGG | - | Chr5:12420879-12420898 | None:intergenic | 35.0% |
| !! | GCCCAATTTTGATCTTTGTA+TGG | + | Chr5:12421456-12421475 | MsG0580024975.01.T01:intron | 35.0% |
| CAATCACCTCCATAAGAGTT+TGG | + | Chr5:12421000-12421019 | MsG0580024975.01.T01:CDS | 40.0% | |
| CAATGATCCAAGTGACATGT+TGG | - | Chr5:12421063-12421082 | None:intergenic | 40.0% | |
| CAATGATCTGAACTTGCCAA+TGG | + | Chr5:12421031-12421050 | MsG0580024975.01.T01:CDS | 40.0% | |
| GATCTTACCAACATGTCACT+TGG | + | Chr5:12421053-12421072 | MsG0580024975.01.T01:CDS | 40.0% | |
| TAAGACAGAAGTGAGTGCAT+TGG | - | Chr5:12421998-12422017 | None:intergenic | 40.0% | |
| TGGACAAACTTCACTGTTGT+AGG | - | Chr5:12422082-12422101 | None:intergenic | 40.0% | |
| ! | ATGGAAGAACTTTCTGGAGA+TGG | + | Chr5:12421947-12421966 | MsG0580024975.01.T01:three_prime_UTR | 40.0% |
| ! | CAACATGTCACTTGGATCAT+TGG | + | Chr5:12421061-12421080 | MsG0580024975.01.T01:CDS | 40.0% |
| ! | GACATGTTGGTAAGATCCAT+TGG | - | Chr5:12421050-12421069 | None:intergenic | 40.0% |
| ! | GCCATAATGGAAGAACTTTC+TGG | + | Chr5:12421941-12421960 | MsG0580024975.01.T01:three_prime_UTR | 40.0% |
| ! | TGCATTGGATGCTGCAATTT+TGG | - | Chr5:12421983-12422002 | None:intergenic | 40.0% |
| !! | AGGAGAAGTACTTGCCATAA+TGG | + | Chr5:12421928-12421947 | MsG0580024975.01.T01:three_prime_UTR | 40.0% |
| !! | GGTCTTAACTTGTGACACAT+CGG | - | Chr5:12421102-12421121 | None:intergenic | 40.0% |
| ATGTCTACCTGGATCAGGAA+TGG | - | Chr5:12420952-12420971 | None:intergenic | 45.0% | |
| CAGTGAAGTTTGTCCACACA+TGG | + | Chr5:12422086-12422105 | MsG0580024975.01.T01:three_prime_UTR | 45.0% | |
| CGGAATTTGTGAACAACTGC+AGG | + | Chr5:12421415-12421434 | MsG0580024975.01.T01:intron | 45.0% | |
| CTGACACTGACAAAGTAGCT+TGG | - | Chr5:12421304-12421323 | None:intergenic | 45.0% | |
| GAGCAATGCAAATGATCAGC+AGG | + | Chr5:12421357-12421376 | MsG0580024975.01.T01:intron | 45.0% | |
| GCATGAACTGTATGCTTGTG+AGG | - | Chr5:12421173-12421192 | None:intergenic | 45.0% | |
| GTCCGACATGGATATGATTG+AGG | + | Chr5:12421668-12421687 | MsG0580024975.01.T01:intron | 45.0% | |
| TATCCATGTCGGACAACCTT+AGG | - | Chr5:12421662-12421681 | None:intergenic | 45.0% | |
| TCTGTATGTCTACCTGGATC+AGG | - | Chr5:12420957-12420976 | None:intergenic | 45.0% | |
| ! | GCATTGACAGATACCATGTG+TGG | - | Chr5:12422102-12422121 | None:intergenic | 45.0% |
| !! | TAAAGGAAAGGAAAGTGCCG+TGG | + | Chr5:12422410-12422429 | MsG0580024975.01.T01:three_prime_UTR | 45.0% |
| GTTCCTAAGGTTGTCCGACA+TGG | + | Chr5:12421656-12421675 | MsG0580024975.01.T01:intron | 50.0% | |
| TGCGTGGATCAACTTGGTTG+CGG | - | Chr5:12421731-12421750 | None:intergenic | 50.0% | |
| TTCCGACAGAAGCTGAGTTC+CGG | - | Chr5:12421400-12421419 | None:intergenic | 50.0% | |
| AGCCGGAACTCAGCTTCTGT+CGG | + | Chr5:12421395-12421414 | MsG0580024975.01.T01:intron | 55.0% | |
| GGTGTCTCCATTCCTGATCC+AGG | + | Chr5:12420942-12420961 | MsG0580024975.01.T01:CDS | 55.0% | |
| !! | GGACGTAAAGCGCTGAGAGC+CGG | + | Chr5:12421378-12421397 | MsG0580024975.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 12420813 | 12422449 | 12420813 | ID=MsG0580024975.01;Name=MsG0580024975.01 |
| Chr5 | mRNA | 12420813 | 12422449 | 12420813 | ID=MsG0580024975.01.T01;Parent=MsG0580024975.01;Name=MsG0580024975.01.T01;_AED=0.29;_eAED=0.64;_QI=0|0|0|1|1|1|2|528|177 |
| Chr5 | exon | 12420813 | 12421345 | 12420813 | ID=MsG0580024975.01.T01:exon:19879;Parent=MsG0580024975.01.T01 |
| Chr5 | exon | 12421921 | 12422449 | 12421921 | ID=MsG0580024975.01.T01:exon:19880;Parent=MsG0580024975.01.T01 |
| Chr5 | CDS | 12420813 | 12421345 | 12420813 | ID=MsG0580024975.01.T01:cds;Parent=MsG0580024975.01.T01 |
| Chr5 | CDS | 12421921 | 12421921 | 12421921 | ID=MsG0580024975.01.T01:cds;Parent=MsG0580024975.01.T01 |
| Chr5 | three_prime_UTR | 12421922 | 12422449 | 12421922 | ID=MsG0580024975.01.T01:three_prime_utr;Parent=MsG0580024975.01.T01 |
| Gene Sequence |
| Protein sequence |
