Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022033.01.T01 | KEH30751.1 | 92.308 | 91 | 6 | 1 | 1 | 90 | 1 | 91 | 4.56E-49 | 168 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022033.01.T01 | Q9C6I6 | 61.29 | 62 | 23 | 1 | 29 | 90 | 36 | 96 | 2.25E-14 | 70.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022033.01.T01 | A0A072UMZ8 | 92.308 | 91 | 6 | 1 | 1 | 90 | 1 | 91 | 2.18e-49 | 168 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480022032.01 | MsG0480022033.01 | 0.884859 | 1.337318e-71 | 8.968675e-68 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022033.01.T01 | MTR_4g080790 | 92.308 | 91 | 6 | 1 | 1 | 90 | 1 | 91 | 5.52e-53 | 168 |
| MsG0480022033.01.T01 | MTR_4g080790 | 92.308 | 91 | 6 | 1 | 1 | 90 | 1 | 91 | 2.65e-52 | 168 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022033.01.T01 | AT1G50940 | 61.290 | 62 | 23 | 1 | 29 | 90 | 36 | 96 | 2.29e-15 | 70.9 |
Find 39 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGTTTCTCTTCTCTTAGC+TGG | 0.364516 | 4:-67235308 | MsG0480022033.01.T01:CDS |
| TCTCTTCTCTTAGCTGGCTC+TGG | 0.387863 | 4:-67235302 | MsG0480022033.01.T01:CDS |
| GAAACAGAAGAGTCGTTGTT+AGG | 0.397977 | 4:+67235323 | None:intergenic |
| TCTGGAACCATGCAATTCTT+AGG | 0.414249 | 4:-67235178 | MsG0480022033.01.T01:CDS |
| GCCTACTCGCAAGTCGCAAA+TGG | 0.422710 | 4:-67235737 | MsG0480022033.01.T01:five_prime_UTR |
| CTCCCACCCTTCCATCTCTC+AGG | 0.427763 | 4:-67235243 | MsG0480022033.01.T01:CDS |
| GGAGAGAGAAGGAGGTTTGA+TGG | 0.437229 | 4:+67235370 | None:intergenic |
| CTCTTCAGAGTGGTGGTTGG+TGG | 0.444815 | 4:+67235609 | None:intergenic |
| TATTATTACGTTTCGAGAAG+TGG | 0.458296 | 4:+67235501 | None:intergenic |
| AGTTACTTACTTATATGCTC+TGG | 0.460507 | 4:-67235196 | MsG0480022033.01.T01:CDS |
| TACGTTTCGAGAAGTGGAAA+AGG | 0.475864 | 4:+67235507 | None:intergenic |
| TATAAGTAAGTAACTGAAAG+AGG | 0.501636 | 4:+67235204 | None:intergenic |
| TGAGTGAGTAACCTGAGAGA+TGG | 0.507897 | 4:+67235232 | None:intergenic |
| TTTCTCTTCAGAGTGGTGGT+TGG | 0.522982 | 4:+67235606 | None:intergenic |
| GTCATCGCGGAGCACGAAAA+CGG | 0.524580 | 4:-67235395 | MsG0480022033.01.T01:CDS |
| GCGTGGAGTGCGGAGAGAGA+AGG | 0.540459 | 4:+67235359 | None:intergenic |
| TTGCGAGTAGGCAGCTGCCA+TGG | 0.569467 | 4:+67235748 | None:intergenic |
| AACCTGAGAGATGGAAGGGT+GGG | 0.578721 | 4:+67235241 | None:intergenic |
| AAAGGATGGAGGTAAGAAGA+AGG | 0.581027 | 4:+67235525 | None:intergenic |
| CTGAGAGATGGAAGGGTGGG+AGG | 0.582498 | 4:+67235244 | None:intergenic |
| TGGAGTGCGGAGAGAGAAGG+AGG | 0.590939 | 4:+67235362 | None:intergenic |
| ACACGTGGCGGCGTGGAGTG+CGG | 0.594993 | 4:+67235349 | None:intergenic |
| GAAGCAGCTTGATGAAGAGA+AGG | 0.597010 | 4:+67235278 | None:intergenic |
| GGAGGCAGCATCACATGCCA+TGG | 0.597945 | 4:-67235765 | MsG0480022033.01.T01:five_prime_UTR |
| TTTCGAGAAGTGGAAAAGGA+TGG | 0.607804 | 4:+67235511 | None:intergenic |
| TAATAAGCCTAAGAATTGCA+TGG | 0.608738 | 4:+67235171 | None:intergenic |
| CGAGAAGTGGAAAAGGATGG+AGG | 0.609170 | 4:+67235514 | None:intergenic |
| GAGTAACCTGAGAGATGGAA+GGG | 0.616601 | 4:+67235237 | None:intergenic |
| TAAGATAGATTATTACACAC+AGG | 0.629317 | 4:-67235710 | MsG0480022033.01.T01:five_prime_UTR |
| TAGGAAGACACGTGGCGGCG+TGG | 0.629518 | 4:+67235342 | None:intergenic |
| TAACCTGAGAGATGGAAGGG+TGG | 0.634315 | 4:+67235240 | None:intergenic |
| GAAAATACAATACAATACAA+TGG | 0.637560 | 4:-67235587 | MsG0480022033.01.T01:exon |
| TGAGTAACCTGAGAGATGGA+AGG | 0.658729 | 4:+67235236 | None:intergenic |
| TCCATTTGCGACTTGCGAGT+AGG | 0.668520 | 4:+67235736 | None:intergenic |
| AACACGACTATTCACGCACA+CGG | 0.671960 | 4:-67235786 | None:intergenic |
| GGGTAGCACTCTTGTCATCG+CGG | 0.684508 | 4:-67235408 | MsG0480022033.01.T01:CDS |
| GCGACTCGCATGATGCTCAG+AGG | 0.688010 | 4:-67235565 | MsG0480022033.01.T01:CDS |
| GTCGTTGTTAGGAAGACACG+TGG | 0.723697 | 4:+67235334 | None:intergenic |
| GTTGTTAGGAAGACACGTGG+CGG | 0.792334 | 4:+67235337 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAAATACAATACAATACAA+TGG | - | Chr4:67235353-67235372 | MsG0480022033.01.T01:CDS | 20.0% |
| ! | ATGAGAACAAAAAAGATGAT+AGG | + | Chr4:67235270-67235289 | None:intergenic | 25.0% |
| ! | TAAGATAGATTATTACACAC+AGG | - | Chr4:67235230-67235249 | MsG0480022033.01.T01:CDS | 25.0% |
| ! | TATAAGTAAGTAACTGAAAG+AGG | + | Chr4:67235739-67235758 | None:intergenic | 25.0% |
| ! | TGAGAACAAAAAAGATGATA+GGG | + | Chr4:67235269-67235288 | None:intergenic | 25.0% |
| AGTTACTTACTTATATGCTC+TGG | - | Chr4:67235744-67235763 | MsG0480022033.01.T01:five_prime_UTR | 30.0% | |
| TAATAAGCCTAAGAATTGCA+TGG | + | Chr4:67235772-67235791 | None:intergenic | 30.0% | |
| TATTATTACGTTTCGAGAAG+TGG | + | Chr4:67235442-67235461 | None:intergenic | 30.0% | |
| ! | ATTGTATTTTCTCTTCAGAG+TGG | + | Chr4:67235344-67235363 | None:intergenic | 30.0% |
| AATTGGATCGATGTAGTGTA+GGG | - | Chr4:67235512-67235531 | MsG0480022033.01.T01:CDS | 35.0% | |
| ATCATTCCACTTTGCTGAAT+TGG | - | Chr4:67235495-67235514 | MsG0480022033.01.T01:intron | 35.0% | |
| AAAGGATGGAGGTAAGAAGA+AGG | + | Chr4:67235418-67235437 | None:intergenic | 40.0% | |
| ATCGATCCAATTCAGCAAAG+TGG | + | Chr4:67235504-67235523 | None:intergenic | 40.0% | |
| GAAACAGAAGAGTCGTTGTT+AGG | + | Chr4:67235620-67235639 | None:intergenic | 40.0% | |
| GAATTGGATCGATGTAGTGT+AGG | - | Chr4:67235511-67235530 | MsG0480022033.01.T01:CDS | 40.0% | |
| TACGTTTCGAGAAGTGGAAA+AGG | + | Chr4:67235436-67235455 | None:intergenic | 40.0% | |
| TTTCGAGAAGTGGAAAAGGA+TGG | + | Chr4:67235432-67235451 | None:intergenic | 40.0% | |
| ! | GTATTTTCTCTTCAGAGTGG+TGG | + | Chr4:67235341-67235360 | None:intergenic | 40.0% |
| ! | TCTGGAACCATGCAATTCTT+AGG | - | Chr4:67235762-67235781 | MsG0480022033.01.T01:five_prime_UTR | 40.0% |
| ! | TCTGTTTCTCTTCTCTTAGC+TGG | - | Chr4:67235632-67235651 | MsG0480022033.01.T01:five_prime_UTR | 40.0% |
| GAAGCAGCTTGATGAAGAGA+AGG | + | Chr4:67235665-67235684 | None:intergenic | 45.0% | |
| GAGTAACCTGAGAGATGGAA+GGG | + | Chr4:67235706-67235725 | None:intergenic | 45.0% | |
| TGAGTAACCTGAGAGATGGA+AGG | + | Chr4:67235707-67235726 | None:intergenic | 45.0% | |
| TGAGTGAGTAACCTGAGAGA+TGG | + | Chr4:67235711-67235730 | None:intergenic | 45.0% | |
| TTTCTCTTCAGAGTGGTGGT+TGG | + | Chr4:67235337-67235356 | None:intergenic | 45.0% | |
| AACCTGAGAGATGGAAGGGT+GGG | + | Chr4:67235702-67235721 | None:intergenic | 50.0% | |
| CGAGAAGTGGAAAAGGATGG+AGG | + | Chr4:67235429-67235448 | None:intergenic | 50.0% | |
| GGAGAGAGAAGGAGGTTTGA+TGG | + | Chr4:67235573-67235592 | None:intergenic | 50.0% | |
| GTCGTTGTTAGGAAGACACG+TGG | + | Chr4:67235609-67235628 | None:intergenic | 50.0% | |
| GTTGTTAGGAAGACACGTGG+CGG | + | Chr4:67235606-67235625 | None:intergenic | 50.0% | |
| TAACCTGAGAGATGGAAGGG+TGG | + | Chr4:67235703-67235722 | None:intergenic | 50.0% | |
| TCCATTTGCGACTTGCGAGT+AGG | + | Chr4:67235207-67235226 | None:intergenic | 50.0% | |
| ! | TCTCTTCTCTTAGCTGGCTC+TGG | - | Chr4:67235638-67235657 | MsG0480022033.01.T01:five_prime_UTR | 50.0% |
| CTCTTCAGAGTGGTGGTTGG+TGG | + | Chr4:67235334-67235353 | None:intergenic | 55.0% | |
| GCCTACTCGCAAGTCGCAAA+TGG | - | Chr4:67235203-67235222 | MsG0480022033.01.T01:CDS | 55.0% | |
| GGGTAGCACTCTTGTCATCG+CGG | - | Chr4:67235532-67235551 | MsG0480022033.01.T01:CDS | 55.0% | |
| GTCATCGCGGAGCACGAAAA+CGG | - | Chr4:67235545-67235564 | MsG0480022033.01.T01:CDS | 55.0% | |
| CTCCCACCCTTCCATCTCTC+AGG | - | Chr4:67235697-67235716 | MsG0480022033.01.T01:five_prime_UTR | 60.0% | |
| CTGAGAGATGGAAGGGTGGG+AGG | + | Chr4:67235699-67235718 | None:intergenic | 60.0% | |
| GCGACTCGCATGATGCTCAG+AGG | - | Chr4:67235375-67235394 | MsG0480022033.01.T01:CDS | 60.0% | |
| GGAGGCAGCATCACATGCCA+TGG | - | Chr4:67235175-67235194 | MsG0480022033.01.T01:CDS | 60.0% | |
| TGGAGTGCGGAGAGAGAAGG+AGG | + | Chr4:67235581-67235600 | None:intergenic | 60.0% | |
| TTGCGAGTAGGCAGCTGCCA+TGG | + | Chr4:67235195-67235214 | None:intergenic | 60.0% | |
| GCGTGGAGTGCGGAGAGAGA+AGG | + | Chr4:67235584-67235603 | None:intergenic | 65.0% | |
| TAGGAAGACACGTGGCGGCG+TGG | + | Chr4:67235601-67235620 | None:intergenic | 65.0% | |
| ACACGTGGCGGCGTGGAGTG+CGG | + | Chr4:67235594-67235613 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 67235163 | 67235799 | 67235163 | ID=MsG0480022033.01;Name=MsG0480022033.01 |
| Chr4 | mRNA | 67235163 | 67235799 | 67235163 | ID=MsG0480022033.01.T01;Parent=MsG0480022033.01;Name=MsG0480022033.01.T01;_AED=0.47;_eAED=0.54;_QI=209|1|0.5|1|0|0|2|0|115 |
| Chr4 | exon | 67235163 | 67235429 | 67235163 | ID=MsG0480022033.01.T01:exon:8223;Parent=MsG0480022033.01.T01 |
| Chr4 | exon | 67235510 | 67235799 | 67235510 | ID=MsG0480022033.01.T01:exon:8222;Parent=MsG0480022033.01.T01 |
| Chr4 | five_prime_UTR | 67235591 | 67235799 | 67235591 | ID=MsG0480022033.01.T01:five_prime_utr;Parent=MsG0480022033.01.T01 |
| Chr4 | CDS | 67235510 | 67235590 | 67235510 | ID=MsG0480022033.01.T01:cds;Parent=MsG0480022033.01.T01 |
| Chr4 | CDS | 67235163 | 67235429 | 67235163 | ID=MsG0480022033.01.T01:cds;Parent=MsG0480022033.01.T01 |
| Gene Sequence |
| Protein sequence |
