AlfalfaGEDB | MsG0480022032.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022032.01

KEGG: K03522
GO: GO:0009055 GO:0050660
PFAM: PF00766 PF01012

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022032.01.T01	XP_003607652.1	98.502	267	4	0	9	275	91	357	0	534

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022032.01.T01	Q9C6I6	75.836	269	64	1	8	275	95	363	1.94E-150	427

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022032.01.T01	G7JDV4	98.502	267	4	0	9	275	91	357	0.0	534

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380015008.01	MsG0480022032.01	0.813843	2.084953e-51	1.376556e-48
MsG0380017247.01	MsG0480022032.01	0.806618	7.537568e-50	4.109859e-47
MsG0380017610.01	MsG0480022032.01	0.823371	1.435770e-53	1.233793e-50
MsG0480020827.01	MsG0480022032.01	0.807138	5.851187e-50	3.233579e-47
MsG0480022032.01	MsG0480022033.01	0.884859	1.337318e-71	8.968675e-68
MsG0480022032.01	MsG0480022610.01	0.817092	3.945765e-52	2.846359e-49
MsG0480022032.01	MsG0780041583.01	0.820536	6.512168e-53	5.166762e-50
MsG0480022032.01	MsG0880047064.01	0.821424	4.067447e-53	3.307915e-50
MsG0480022032.01	MsG0880047296.01	0.856133	3.951519e-62	9.335973e-59

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022032.01.T01	MTR_4g080790	98.502	267	4	0	9	275	91	357	0.0	534
MsG0480022032.01.T01	MTR_4g080790	97.740	177	4	0	9	185	91	267	5.08e-123	353

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022032.01.T01	AT1G50940	75.836	269	64	1	8	275	95	363	1.98e-151	427

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Find 68 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCAATTACCTGAAATAT+AGG	0.367457	4:+67231025	None:intergenic
AAATGGACTAGTTTAGCCCA+GGG	0.633679	4:+67234396	None:intergenic
AAGGACTGGCACCAGTATAT+CGG	0.456890	4:+67232954	None:intergenic
AATGCACGGATTGTAGTTAC+TGG	0.510808	4:-67232503	MsG0480022032.01.T01:CDS
AATTGTTAATATACAAGGAC+TGG	0.548727	4:+67232940	None:intergenic
ACCAAAAGCCATATAGAGTT+CGG	0.403600	4:+67231126	None:intergenic
ACCAGCAGCACCAAGTTTCT+TGG	0.273868	4:+67232430	None:intergenic
ACTAGTAGGAGATTTATTCG+AGG	0.644611	4:-67230885	MsG0480022032.01.T01:CDS
ACTCGTGCTGCTGTTGATGC+AGG	0.488675	4:-67231962	MsG0480022032.01.T01:CDS
AGAACCCATTGGCAGAACCC+TGG	0.524794	4:-67234413	MsG0480022032.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATGAAAAACCTGTAAACC+TGG	-	Chr4:67231630-67231649	MsG0480022032.01.T01:intron	30.0%
ACAACTCATGAGTTCAAAAT+CGG	+	Chr4:67231752-67231771	None:intergenic	30.0%
ACAATTAGCAGCATAATCAT+GGG	+	Chr4:67233469-67233488	None:intergenic	30.0%
AGAATAACAATACCAAACAG+AGG	+	Chr4:67231190-67231209	None:intergenic	30.0%
AGGAAAATGAAGTCTATTAG+TGG	-	Chr4:67234507-67234526	MsG0480022032.01.T01:intron	30.0%
CACTTGATCTGATATTAATC+TGG	-	Chr4:67231593-67231612	MsG0480022032.01.T01:intron	30.0%
GAATAACAATACCAAACAGA+GGG	+	Chr4:67231189-67231208	None:intergenic	30.0%
GAATAACTTTGCATGTGTTA+AGG	-	Chr4:67232023-67232042	MsG0480022032.01.T01:intron	30.0%
GGTATATATCTCAAACTTCT+CGG	-	Chr4:67232679-67232698	MsG0480022032.01.T01:intron	30.0%
GTATATATCTCAAACTTCTC+GGG	-	Chr4:67232680-67232699	MsG0480022032.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	67230656	67234595	67230656	ID=MsG0480022032.01;Name=MsG0480022032.01
Chr4	mRNA	67230656	67234595	67230656	ID=MsG0480022032.01.T01;Parent=MsG0480022032.01;Name=MsG0480022032.01.T01;_AED=0.45;_eAED=0.46;_QI=0\|0.83\|0.85\|1\|1\|1\|7\|188\|275
Chr4	exon	67230656	67230921	67230656	ID=MsG0480022032.01.T01:exon:8221;Parent=MsG0480022032.01.T01
Chr4	exon	67231036	67231176	67231036	ID=MsG0480022032.01.T01:exon:8220;Parent=MsG0480022032.01.T01
Chr4	exon	67231940	67231992	67231940	ID=MsG0480022032.01.T01:exon:8219;Parent=MsG0480022032.01.T01
Chr4	exon	67232432	67232587	67232432	ID=MsG0480022032.01.T01:exon:8218;Parent=MsG0480022032.01.T01
Chr4	exon	67232846	67233009	67232846	ID=MsG0480022032.01.T01:exon:8217;Parent=MsG0480022032.01.T01
Chr4	exon	67234252	67234463	67234252	ID=MsG0480022032.01.T01:exon:8216;Parent=MsG0480022032.01.T01
Chr4	exon	67234572	67234595	67234572	ID=MsG0480022032.01.T01:exon:8215;Parent=MsG0480022032.01.T01
Chr4	CDS	67234572	67234595	67234572	ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4	CDS	67234252	67234463	67234252	ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4	CDS	67232846	67233009	67232846	ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4	CDS	67232432	67232587	67232432	ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4	CDS	67231940	67231992	67231940	ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4	CDS	67231036	67231176	67231036	ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4	CDS	67230844	67230921	67230844	ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4	three_prime_UTR	67230656	67230843	67230656	ID=MsG0480022032.01.T01:three_prime_utr;Parent=MsG0480022032.01.T01

Gene Sequence

>MsG0480022032.01.T01

ATGATAAAAAGAGGAAAAGAAAATGTGCTCGTGGCGGATTCAGATACATTCAAGAACCCATTGGCAGAACCCTGGGCTAAACTAGTCCATTTGGTTCAGCAAAGAGGTGGTTACTCTCATATAATTGCAGCTTCAAATTCTTTTGGGAAAAATGTCATGCCGCGTGCTGCTGCTCTTTTAGATGTTTCTCCAGTTACTGATGTTACAGGAATATCTGATTCTCATACTTTTATAAGGCCAATATATGCAGGAAATGCACTTTGTACAGTCCGATATACTGGTGCCAGTCCTTGTATATTAACAATTAGGTCAACTTCTTTTCCTGTTTCTCAGAAGCCTGTTGACTCAAAATCTGATAAGGCTTCCATATCTCAGGTTGATCTTTCAACCTTTGACGAAGATCTGGACAAGTCTAGGTATATATCTCAAACTTCTCGGGATGATGAAGGACCAGACCTTGGGAATGCACGGATTGTAGTTACTGGTGGTCGTGCACTAAAGAGTGCTGAGAACTTTAAGTTGATCGAAAATCTAGCCAAGAAACTTGGTGCTGCTGTTGGTGCTACTCGTGCTGCTGTTGATGCAGGATTTGTTGCTAATGATCTCCAGGTTGGACAAACTGGAAAGATTGTTGCTCCCGAACTCTATATGGCTTTTGGTGTATCTGGAGCTATTCAACATATAGCAGGGATGAGGGATTCCAAAGTCATTGTTGCTGTGAACAACGATGCTGATGCTCCTATATTTCAGGTTGCTGATTATGGACTAGTAGGAGATTTATTCGAGGTGCTACCAGAGTTGTTGGAAAAGCTTCCTGAGAAAAAATAA

Protein sequence

>MsG0480022032.01.T01

MIKRGKENVLVADSDTFKNPLAEPWAKLVHLVQQRGGYSHIIAASNSFGKNVMPRAAALLDVSPVTDVTGISDSHTFIRPIYAGNALCTVRYTGASPCILTIRSTSFPVSQKPVDSKSDKASISQVDLSTFDEDLDKSRYISQTSRDDEGPDLGNARIVVTGGRALKSAENFKLIENLAKKLGAAVGATRAAVDAGFVANDLQVGQTGKIVAPELYMAFGVSGAIQHIAGMRDSKVIVAVNNDADAPIFQVADYGLVGDLFEVLPELLEKLPEKK*