AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022032.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022032.01.T01 MTR_4g080790 98.502 267 4 0 9 275 91 357 0.0 534
MsG0480022032.01.T01 MTR_4g080790 97.740 177 4 0 9 185 91 267 5.08e-123 353
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022032.01.T01 AT1G50940 75.836 269 64 1 8 275 95 363 1.98e-151 427

Find 68 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCAGCTTCAAATTCTTT+TGG 0.232309 4:-67234342 MsG0480022032.01.T01:CDS
TGCTGATGCTCCTATATTTC+AGG 0.232369 4:-67231035 MsG0480022032.01.T01:intron
ACCAGCAGCACCAAGTTTCT+TGG 0.273868 4:+67232430 None:intergenic
CCCGAACTCTATATGGCTTT+TGG 0.284745 4:-67231127 MsG0480022032.01.T01:CDS
TCTCCAGTTACTGATGTTAC+AGG 0.286599 4:-67234279 MsG0480022032.01.T01:CDS
GAACTACAGGTTGCTGATTA+TGG 0.290961 4:-67230908 MsG0480022032.01.T01:intron
TGTTGCTCCCGAACTCTATA+TGG 0.302664 4:-67231134 MsG0480022032.01.T01:CDS
AGTGCATTTCCTGCATATAT+TGG 0.304541 4:+67232986 None:intergenic
TATATGAAGGTTGGACAAAC+TGG 0.344212 4:-67231163 MsG0480022032.01.T01:intron
GTCCTTGTATATTAACAATT+AGG 0.357874 4:-67232937 MsG0480022032.01.T01:CDS
GTTGCTTAATGTTTCATCCT+TGG 0.361639 4:-67230787 MsG0480022032.01.T01:three_prime_UTR
AAAGCAATTACCTGAAATAT+AGG 0.367457 4:+67231025 None:intergenic
CTTTGTACAGTCCGATATAC+TGG 0.386395 4:-67232965 MsG0480022032.01.T01:CDS
TCAACAGGCTTCTGAGAAAC+AGG 0.395915 4:+67232902 None:intergenic
ACCAAAAGCCATATAGAGTT+CGG 0.403600 4:+67231126 None:intergenic
CTGGGCTAAACTAGTCCATT+TGG 0.420011 4:-67234394 MsG0480022032.01.T01:CDS
TGAAAGATCAACCTGAGATA+TGG 0.422186 4:+67232859 None:intergenic
GGTATATATCTCAAACTTCT+CGG 0.422410 4:-67232550 MsG0480022032.01.T01:CDS
ATTCCTGTAACATCAGTAAC+TGG 0.428271 4:+67234276 None:intergenic
GCCAAGAAACTTGGTGCTGC+TGG 0.432419 4:-67232431 MsG0480022032.01.T01:intron
CGAGGTGCTACCAGAGTTGT+TGG 0.446069 4:-67230867 MsG0480022032.01.T01:CDS
CCAAAAGCCATATAGAGTTC+GGG 0.449038 4:+67231127 None:intergenic
CACCTCTTTGCTGAACCAAA+TGG 0.450358 4:+67234379 None:intergenic
TAGCCCAGGGTTCTGCCAAT+GGG 0.450731 4:+67234409 None:intergenic
AAGGACTGGCACCAGTATAT+CGG 0.456890 4:+67232954 None:intergenic
GTTCACAGCAACAATGACTT+TGG 0.458974 4:+67231063 None:intergenic
AGGAAAATGAAGTCTATTAG+TGG 0.461919 4:-67230722 MsG0480022032.01.T01:three_prime_UTR
GTTGCTGATTATGGACTAGT+AGG 0.465111 4:-67230899 MsG0480022032.01.T01:CDS
ATCCGTGCATTCCCAAGGTC+TGG 0.468558 4:+67232515 None:intergenic
TTAGCCCAGGGTTCTGCCAA+TGG 0.475265 4:+67234408 None:intergenic
TGATAAGGCTTCCATATCTC+AGG 0.477728 4:-67232870 MsG0480022032.01.T01:CDS
ACTCGTGCTGCTGTTGATGC+AGG 0.488675 4:-67231962 MsG0480022032.01.T01:CDS
GTATATATCTCAAACTTCTC+GGG 0.489381 4:-67232549 MsG0480022032.01.T01:CDS
TGACAGATCTGGACAAGTCT+AGG 0.502015 4:-67232571 MsG0480022032.01.T01:intron
TGTTGACTCAAAATCTGATA+AGG 0.502932 4:-67232885 MsG0480022032.01.T01:CDS
GGAGCTATTCAACATATAGC+AGG 0.502950 4:-67231097 MsG0480022032.01.T01:CDS
ATGATGAAGGACCAGACCTT+GGG 0.508260 4:-67232526 MsG0480022032.01.T01:CDS
ATTTGTTGCTAATGATCTCC+AGG 0.509284 4:-67231939 MsG0480022032.01.T01:intron
AATGCACGGATTGTAGTTAC+TGG 0.510808 4:-67232503 MsG0480022032.01.T01:CDS
CAAACTTCTCGGGATGATGA+AGG 0.521219 4:-67232539 MsG0480022032.01.T01:CDS
GATGATGAAGGACCAGACCT+TGG 0.521865 4:-67232527 MsG0480022032.01.T01:CDS
AGAACCCATTGGCAGAACCC+TGG 0.524794 4:-67234413 MsG0480022032.01.T01:CDS
GAACCCATTGGCAGAACCCT+GGG 0.532115 4:-67234412 MsG0480022032.01.T01:CDS
CAAATGGACTAGTTTAGCCC+AGG 0.533498 4:+67234395 None:intergenic
GAAAATCTAGCCAAGAAACT+TGG 0.537062 4:-67232440 MsG0480022032.01.T01:CDS
GATCTTTCAACCTTTGACGA+AGG 0.548642 4:-67232845 MsG0480022032.01.T01:intron
AATTGTTAATATACAAGGAC+TGG 0.548727 4:+67232940 None:intergenic
GACCTAATTGTTAATATACA+AGG 0.553893 4:+67232935 None:intergenic
TCATACAGGCCAATATATGC+AGG 0.554146 4:-67232995 MsG0480022032.01.T01:intron
GTCCATTTGGTTCAGCAAAG+AGG 0.561354 4:-67234381 MsG0480022032.01.T01:CDS
TTCAACATATAGCAGGGATG+AGG 0.567627 4:-67231090 MsG0480022032.01.T01:CDS
GACCAGACCTTGGGAATGCA+CGG 0.569448 4:-67232517 MsG0480022032.01.T01:CDS
ATTGTGAAATTGTATTTCCA+AGG 0.581089 4:+67230770 None:intergenic
GATAAACAGACCTTCGTCAA+AGG 0.586100 4:+67232835 None:intergenic
TTAGTGGAACATTATACTAC+CGG 0.586275 4:-67230706 MsG0480022032.01.T01:three_prime_UTR
TCAACATATAGCAGGGATGA+GGG 0.589099 4:-67231089 MsG0480022032.01.T01:CDS
AGATACATTCAAGAACCCAT+TGG 0.600709 4:-67234424 MsG0480022032.01.T01:CDS
GCTTGTGGTGTAGGTGCTCG+TGG 0.604147 4:-67234454 MsG0480022032.01.T01:intron
CTACAATCCGTGCATTCCCA+AGG 0.606621 4:+67232510 None:intergenic
CATTTGGTTCAGCAAAGAGG+TGG 0.612054 4:-67234378 MsG0480022032.01.T01:CDS
GAGCTATTCAACATATAGCA+GGG 0.627640 4:-67231096 MsG0480022032.01.T01:CDS
AAATGGACTAGTTTAGCCCA+GGG 0.633679 4:+67234396 None:intergenic
ACTAGTAGGAGATTTATTCG+AGG 0.644611 4:-67230885 MsG0480022032.01.T01:CDS
ATAATGCAAAATGAGCCTAC+CGG 0.658046 4:+67230687 None:intergenic
TCTAAAAGAGCAGCAGCACG+CGG 0.669095 4:+67234306 None:intergenic
GCACGGATTGTAGTTACTGG+TGG 0.675072 4:-67232500 MsG0480022032.01.T01:CDS
TGGAACATTATACTACCGGT+AGG 0.680103 4:-67230702 MsG0480022032.01.T01:three_prime_UTR
TGTGGTGTAGGTGCTCGTGG+CGG 0.764994 4:-67234451 MsG0480022032.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTAAAAACTTAAAAGT+CGG - Chr4:67233537-67233556 MsG0480022032.01.T01:intron 10.0%
!!! GTTTTTTAATTTTATTGTTT+TGG - Chr4:67232096-67232115 MsG0480022032.01.T01:intron 10.0%
!!! TATTTAATTTTGAATTTGAA+AGG + Chr4:67233326-67233345 None:intergenic 10.0%
!! AAAAAGAAATAATAAATCTC+TGG + Chr4:67232866-67232885 None:intergenic 15.0%
!! TATTTATTAAGATTTAAGTG+TGG + Chr4:67231851-67231870 None:intergenic 15.0%
!! TTCATATACAATTTGAAAAT+AGG + Chr4:67234054-67234073 None:intergenic 15.0%
!!! ATTTATTATTTCTTTTTTGC+TGG - Chr4:67232869-67232888 MsG0480022032.01.T01:CDS 15.0%
!!! TATTTTCAAATTGTATATGA+AGG - Chr4:67234053-67234072 MsG0480022032.01.T01:intron 15.0%
!!! TTTATATTTTGATTTCATAC+AGG - Chr4:67232220-67232239 MsG0480022032.01.T01:intron 15.0%
!! AACAAATAAACAAGTTACAA+GGG + Chr4:67231958-67231977 None:intergenic 20.0%
!! AACATTTATATTTCATAAGC+AGG + Chr4:67232176-67232195 None:intergenic 20.0%
!! ACAAAACAAACAATATGATA+CGG + Chr4:67233196-67233215 None:intergenic 20.0%
!! ACATACGTATTATAGATTTA+TGG - Chr4:67231297-67231316 MsG0480022032.01.T01:intron 20.0%
!! ATATACATGAACCAAATTTA+CGG + Chr4:67231809-67231828 None:intergenic 20.0%
!! ATTTGAATATGCATTATAGT+TGG - Chr4:67233953-67233972 MsG0480022032.01.T01:intron 20.0%
!! GCTTATGAAATATAAATGTT+GGG - Chr4:67232176-67232195 MsG0480022032.01.T01:intron 20.0%
!! TATTTATAGAGCTAAAAAGT+CGG - Chr4:67232048-67232067 MsG0480022032.01.T01:intron 20.0%
!! TGCTTATGAAATATAAATGT+TGG - Chr4:67232175-67232194 MsG0480022032.01.T01:intron 20.0%
!! TTTGAATATGCATTATAGTT+GGG - Chr4:67233954-67233973 MsG0480022032.01.T01:intron 20.0%
!!! TACACAATTTTCTATGTAAA+AGG + Chr4:67233008-67233027 None:intergenic 20.0%
!!! TTTTAGCAAGTTTTGTTATT+TGG - Chr4:67233731-67233750 MsG0480022032.01.T01:intron 20.0%
! AAAAACTTAAAAGTCGGAAA+TGG - Chr4:67233543-67233562 MsG0480022032.01.T01:intron 25.0%
! AAAGCAATTACCTGAAATAT+AGG + Chr4:67234207-67234226 None:intergenic 25.0%
! AATGAACTGGAATTTAAATC+AGG + Chr4:67232910-67232929 None:intergenic 25.0%
! AATTGTTAATATACAAGGAC+TGG + Chr4:67232292-67232311 None:intergenic 25.0%
! AGCTTATCATACTAAAATTG+TGG + Chr4:67231496-67231515 None:intergenic 25.0%
! ATGAACTGGAATTTAAATCA+GGG + Chr4:67232909-67232928 None:intergenic 25.0%
! ATGATGTATGGATCTATATT+AGG - Chr4:67233818-67233837 MsG0480022032.01.T01:intron 25.0%
! ATTGTGAAATTGTATTTCCA+AGG + Chr4:67234462-67234481 None:intergenic 25.0%
! CAAATCGATGTAAATAACTA+AGG + Chr4:67232562-67232581 None:intergenic 25.0%
! CAATTTGAAAATAGGAAACA+CGG + Chr4:67234046-67234065 None:intergenic 25.0%
! CTATAGTCAACAGATAAATA+AGG + Chr4:67232485-67232504 None:intergenic 25.0%
! GAACAAATAAACAAGTTACA+AGG + Chr4:67231959-67231978 None:intergenic 25.0%
! GACCTAATTGTTAATATACA+AGG + Chr4:67232297-67232316 None:intergenic 25.0%
! GCTTATCATACTAAAATTGT+GGG + Chr4:67231495-67231514 None:intergenic 25.0%
! GGAATAGATAGATAATTTAC+TGG - Chr4:67233592-67233611 MsG0480022032.01.T01:intron 25.0%
! GTCCTTGTATATTAACAATT+AGG - Chr4:67232292-67232311 MsG0480022032.01.T01:intron 25.0%
! TTCAAATTGTATATGAAGGT+TGG - Chr4:67234057-67234076 MsG0480022032.01.T01:intron 25.0%
! TTTAAATTCCAGTTCATTGA+TGG - Chr4:67232912-67232931 MsG0480022032.01.T01:CDS 25.0%
!! AAGAAAATGTAAGTTGAGAA+AGG - Chr4:67230672-67230691 MsG0480022032.01.T01:three_prime_UTR 25.0%
!! AGAAAATGTAAGTTGAGAAA+GGG - Chr4:67230673-67230692 MsG0480022032.01.T01:three_prime_UTR 25.0%
!! ATTTTCTGTCAAGTATAACA+TGG - Chr4:67233675-67233694 MsG0480022032.01.T01:intron 25.0%
!! ATTTTGCATTATTACTACAG+TGG - Chr4:67234553-67234572 MsG0480022032.01.T01:intron 25.0%
!! CTGATTCTCATACTTTTATA+AGG - Chr4:67230978-67230997 MsG0480022032.01.T01:intron 25.0%
!!! AAACTTGCTAAAAGAATGAA+AGG + Chr4:67233724-67233743 None:intergenic 25.0%
!!! CACACATTTTATTTTTTCTC+AGG + Chr4:67234396-67234415 None:intergenic 25.0%
!!! CTTTATTATTTTGACAGATC+TGG - Chr4:67232647-67232666 MsG0480022032.01.T01:intron 25.0%
!!! TATTTCAGGTAATTGCTTTT+TGG - Chr4:67234208-67234227 MsG0480022032.01.T01:intron 25.0%
!!! TCTTTTATCAAGTTGTAGTT+TGG + Chr4:67233380-67233399 None:intergenic 25.0%
!!! TGCATTGACAATTTGTTTTT+GGG - Chr4:67232426-67232445 MsG0480022032.01.T01:intron 25.0%
AAATGAAAAACCTGTAAACC+TGG - Chr4:67231630-67231649 MsG0480022032.01.T01:intron 30.0%
ACAACTCATGAGTTCAAAAT+CGG + Chr4:67231752-67231771 None:intergenic 30.0%
ACAATTAGCAGCATAATCAT+GGG + Chr4:67233469-67233488 None:intergenic 30.0%
AGAATAACAATACCAAACAG+AGG + Chr4:67231190-67231209 None:intergenic 30.0%
AGGAAAATGAAGTCTATTAG+TGG - Chr4:67234507-67234526 MsG0480022032.01.T01:intron 30.0%
CACTTGATCTGATATTAATC+TGG - Chr4:67231593-67231612 MsG0480022032.01.T01:intron 30.0%
GAATAACAATACCAAACAGA+GGG + Chr4:67231189-67231208 None:intergenic 30.0%
GAATAACTTTGCATGTGTTA+AGG - Chr4:67232023-67232042 MsG0480022032.01.T01:intron 30.0%
GGTATATATCTCAAACTTCT+CGG - Chr4:67232679-67232698 MsG0480022032.01.T01:intron 30.0%
GTATATATCTCAAACTTCTC+GGG - Chr4:67232680-67232699 MsG0480022032.01.T01:intron 30.0%
TAATACTATCCCATTAGCTA+TGG + Chr4:67231999-67232018 None:intergenic 30.0%
TACTGGTCAATTATCACTTT+GGG - Chr4:67233609-67233628 MsG0480022032.01.T01:intron 30.0%
TAGCGATTAGGCTATATTTA+TGG - Chr4:67231227-67231246 MsG0480022032.01.T01:intron 30.0%
TCAAATTAACCTCTAGCATA+AGG + Chr4:67233847-67233866 None:intergenic 30.0%
TGATATTAATCTGGATTCTG+AGG - Chr4:67231602-67231621 MsG0480022032.01.T01:intron 30.0%
TGGAAGGGAGATTTAAATTT+GGG - Chr4:67230725-67230744 MsG0480022032.01.T01:three_prime_UTR 30.0%
TTACTGGTCAATTATCACTT+TGG - Chr4:67233608-67233627 MsG0480022032.01.T01:intron 30.0%
TTAGTGGAACATTATACTAC+CGG - Chr4:67234523-67234542 MsG0480022032.01.T01:intron 30.0%
TTGATAAAAGATGAATGACG+AGG - Chr4:67233389-67233408 MsG0480022032.01.T01:intron 30.0%
TTGGAAGGGAGATTTAAATT+TGG - Chr4:67230724-67230743 MsG0480022032.01.T01:three_prime_UTR 30.0%
TTGTGTGTGTATTGTTTCAT+GGG + Chr4:67233350-67233369 None:intergenic 30.0%
TTGTTTACCTGAAAAAGTAG+AGG - Chr4:67234487-67234506 MsG0480022032.01.T01:intron 30.0%
TTTGATGCTACAAAGATCAA+TGG - Chr4:67232512-67232531 MsG0480022032.01.T01:CDS 30.0%
TTTGTGTGTGTATTGTTTCA+TGG + Chr4:67233351-67233370 None:intergenic 30.0%
! AAGACAAAGTTTCTTTTCGT+GGG - Chr4:67233062-67233081 MsG0480022032.01.T01:intron 30.0%
! ATGGCATGGAGAATATTTTA+AGG - Chr4:67232931-67232950 MsG0480022032.01.T01:CDS 30.0%
! ATTGCAGCTTCAAATTCTTT+TGG - Chr4:67230887-67230906 MsG0480022032.01.T01:CDS 30.0%
! GAAAATGTAAGTTGAGAAAG+GGG - Chr4:67230674-67230693 MsG0480022032.01.T01:three_prime_UTR 30.0%
! GTTAATTTGATTCTTCAGCT+TGG - Chr4:67233857-67233876 MsG0480022032.01.T01:intron 30.0%
! TTGCAGCTTCAAATTCTTTT+GGG - Chr4:67230888-67230907 MsG0480022032.01.T01:CDS 30.0%
!! GTCAATTCCATTTTAGTACA+TGG - Chr4:67234001-67234020 MsG0480022032.01.T01:intron 30.0%
!! GTGCATTGACAATTTGTTTT+TGG - Chr4:67232425-67232444 MsG0480022032.01.T01:intron 30.0%
!! TGTTGACTCAAAATCTGATA+AGG - Chr4:67232344-67232363 MsG0480022032.01.T01:intron 30.0%
!! TTATCAGATTTTGAGTCAAC+AGG + Chr4:67232345-67232364 None:intergenic 30.0%
!!! GCGTTAACTTTATTTTTGTG+AGG - Chr4:67231002-67231021 MsG0480022032.01.T01:intron 30.0%
!!! TCATTTTCCTCTACTTTTTC+AGG + Chr4:67234497-67234516 None:intergenic 30.0%
!!! TTTTTTGCTGGATTCACAAT+AGG - Chr4:67232881-67232900 MsG0480022032.01.T01:CDS 30.0%
AAAAAGTCGGCTAACAAAGT+AGG - Chr4:67232061-67232080 MsG0480022032.01.T01:intron 35.0%
AAAAGTCGGCTAACAAAGTA+GGG - Chr4:67232062-67232081 MsG0480022032.01.T01:intron 35.0%
AAACACGCTATCATGTGTTA+AGG + Chr4:67233159-67233178 None:intergenic 35.0%
AAATGCTAACAAGTTCCCTT+AGG - Chr4:67232955-67232974 MsG0480022032.01.T01:CDS 35.0%
AACTAGGCCATGTACTAAAA+TGG + Chr4:67234011-67234030 None:intergenic 35.0%
AATACCTACCCTAAGTTTGA+AGG + Chr4:67231673-67231692 None:intergenic 35.0%
AATGCTAACAAGTTCCCTTA+GGG - Chr4:67232956-67232975 MsG0480022032.01.T01:CDS 35.0%
AATGGAAAAACATCTGTGTG+TGG - Chr4:67233561-67233580 MsG0480022032.01.T01:intron 35.0%
AATTTCACCTCCATAGCTAA+TGG - Chr4:67231986-67232005 MsG0480022032.01.T01:intron 35.0%
ACCAAAAGCCATATAGAGTT+CGG + Chr4:67234106-67234125 None:intergenic 35.0%
ACGGATGTGAGAATAAAACT+AGG + Chr4:67234027-67234046 None:intergenic 35.0%
ACTAGTAGGAGATTTATTCG+AGG - Chr4:67234344-67234363 MsG0480022032.01.T01:CDS 35.0%
AGATACATTCAAGAACCCAT+TGG - Chr4:67230805-67230824 MsG0480022032.01.T01:three_prime_UTR 35.0%
AGCTATGGAGGTGAAATTAA+CGG + Chr4:67231984-67232003 None:intergenic 35.0%
AGGTCTTGATACAAAACATC+AGG - Chr4:67231726-67231745 MsG0480022032.01.T01:intron 35.0%
AGTGCATTTCCTGCATATAT+TGG + Chr4:67232246-67232265 None:intergenic 35.0%
ATAATGCAAAATGAGCCTAC+CGG + Chr4:67234545-67234564 None:intergenic 35.0%
ATACTAAAATTGTGGGCAAG+TGG + Chr4:67231488-67231507 None:intergenic 35.0%
ATCAATGGATCATCTGTATC+AGG - Chr4:67232527-67232546 MsG0480022032.01.T01:CDS 35.0%
ATTCCTGTAACATCAGTAAC+TGG + Chr4:67230956-67230975 None:intergenic 35.0%
ATTGCTTAAACAATGCCCTA+AGG + Chr4:67232974-67232993 None:intergenic 35.0%
ATTTCACCTCCATAGCTAAT+GGG - Chr4:67231987-67232006 MsG0480022032.01.T01:intron 35.0%
CACTATTACATCGTCATGAT+TGG - Chr4:67231099-67231118 MsG0480022032.01.T01:CDS 35.0%
CGATTAGGCTATATTTATGG+AGG - Chr4:67231230-67231249 MsG0480022032.01.T01:intron 35.0%
CTATCAACTGTTTAGCGATT+AGG - Chr4:67231215-67231234 MsG0480022032.01.T01:intron 35.0%
GAAAATCTAGCCAAGAAACT+TGG - Chr4:67232789-67232808 MsG0480022032.01.T01:intron 35.0%
GAAGGGAGATTTAAATTTGG+GGG - Chr4:67230727-67230746 MsG0480022032.01.T01:three_prime_UTR 35.0%
GAAGTGATACACCGTAAATT+TGG - Chr4:67231795-67231814 MsG0480022032.01.T01:intron 35.0%
GACAATTAGCAGCATAATCA+TGG + Chr4:67233470-67233489 None:intergenic 35.0%
GAGCTATTCAACATATAGCA+GGG - Chr4:67234133-67234152 MsG0480022032.01.T01:intron 35.0%
GATATGTGAACAATTGCAAG+TGG - Chr4:67232607-67232626 MsG0480022032.01.T01:intron 35.0%
GGAAGGGAGATTTAAATTTG+GGG - Chr4:67230726-67230745 MsG0480022032.01.T01:three_prime_UTR 35.0%
GGCAAATTGAAAACACAAGT+AGG - Chr4:67232447-67232466 MsG0480022032.01.T01:CDS 35.0%
GTACTAAAATGGAATTGACG+TGG + Chr4:67234000-67234019 None:intergenic 35.0%
GTTGCTTAATGTTTCATCCT+TGG - Chr4:67234442-67234461 MsG0480022032.01.T01:CDS 35.0%
TATATGAAGGTTGGACAAAC+TGG - Chr4:67234066-67234085 MsG0480022032.01.T01:intron 35.0%
TCAATGGATCATCTGTATCA+GGG - Chr4:67232528-67232547 MsG0480022032.01.T01:CDS 35.0%
TGAAAGATCAACCTGAGATA+TGG + Chr4:67232373-67232392 None:intergenic 35.0%
TGCAATGGCATATCATTTGA+GGG - Chr4:67233930-67233949 MsG0480022032.01.T01:intron 35.0%
TGTATTGCACAATTCAACAC+TGG - Chr4:67231075-67231094 MsG0480022032.01.T01:CDS 35.0%
TGTGAAAGTCTAAACGGTTA+TGG + Chr4:67231927-67231946 None:intergenic 35.0%
TGTGTGTGTATTGTTTCATG+GGG + Chr4:67233349-67233368 None:intergenic 35.0%
TTCACATATCCAAGTCTGAA+GGG + Chr4:67232597-67232616 None:intergenic 35.0%
TTCCTTAAACAATGCCCTTA+GGG - Chr4:67233085-67233104 MsG0480022032.01.T01:intron 35.0%
TTGAAAGGAAGTTAAGTACC+TGG + Chr4:67233311-67233330 None:intergenic 35.0%
TTGCTTAAACAATGCCCTAA+GGG + Chr4:67232973-67232992 None:intergenic 35.0%
! ATTTGTTGCTAATGATCTCC+AGG - Chr4:67233290-67233309 MsG0480022032.01.T01:intron 35.0%
! CAAGACAAAGTTTCTTTTCG+TGG - Chr4:67233061-67233080 MsG0480022032.01.T01:intron 35.0%
! TATCTGTTGACTATAGATCC+TGG - Chr4:67232489-67232508 MsG0480022032.01.T01:CDS 35.0%
!! GGGGTTTTGATAGTTTACTT+GGG - Chr4:67230746-67230765 MsG0480022032.01.T01:three_prime_UTR 35.0%
!! TATATGGCTTTTGGTGTATC+TGG - Chr4:67234111-67234130 MsG0480022032.01.T01:intron 35.0%
AATTACTTCCCCTTCAGACT+TGG - Chr4:67232585-67232604 MsG0480022032.01.T01:intron 40.0%
ACATGAGGCAAAACTGTTTC+AGG + Chr4:67234293-67234312 None:intergenic 40.0%
AGCAGCGCAACTATAAATCT+AGG - Chr4:67231456-67231475 MsG0480022032.01.T01:intron 40.0%
AGGACCTATCCTTCAAACTT+AGG - Chr4:67231661-67231680 MsG0480022032.01.T01:intron 40.0%
ATGGAAAATCTCACTCCCTT+TGG - Chr4:67231316-67231335 MsG0480022032.01.T01:intron 40.0%
ATTTGACAAGACCCTCTGTT+TGG - Chr4:67231175-67231194 MsG0480022032.01.T01:intron 40.0%
CAGCATAATCATGGGAGAAA+CGG + Chr4:67233461-67233480 None:intergenic 40.0%
CATGTGATATGCTGAACTAC+AGG - Chr4:67234308-67234327 MsG0480022032.01.T01:CDS 40.0%
CATTCTCTCCTTTGTTCAGT+TGG - Chr4:67233240-67233259 MsG0480022032.01.T01:intron 40.0%
CCAAAAGCCATATAGAGTTC+GGG + Chr4:67234105-67234124 None:intergenic 40.0%
CCTGCTTGTGAAAGTCTAAA+CGG + Chr4:67231933-67231952 None:intergenic 40.0%
CGAACGTTAAGTATCTAGTC+AGG + Chr4:67233910-67233929 None:intergenic 40.0%
CTACCCTAAGTTTGAAGGAT+AGG + Chr4:67231668-67231687 None:intergenic 40.0%
CTATCCTTCAAACTTAGGGT+AGG - Chr4:67231666-67231685 MsG0480022032.01.T01:intron 40.0%
CTTTGTACAGTCCGATATAC+TGG - Chr4:67232264-67232283 MsG0480022032.01.T01:intron 40.0%
GAAGGGTTGGATATGATGTA+TGG - Chr4:67233806-67233825 MsG0480022032.01.T01:intron 40.0%
GATAAACAGACCTTCGTCAA+AGG + Chr4:67232397-67232416 None:intergenic 40.0%
GATACTTAACGTTCGTGCAA+TGG - Chr4:67233915-67233934 MsG0480022032.01.T01:intron 40.0%
GATCTTTCAACCTTTGACGA+AGG - Chr4:67232384-67232403 MsG0480022032.01.T01:intron 40.0%
GATCTTTGTAGCATCAAACC+AGG + Chr4:67232510-67232529 None:intergenic 40.0%
GGACCTATCCTTCAAACTTA+GGG - Chr4:67231662-67231681 MsG0480022032.01.T01:intron 40.0%
GGAGCTATTCAACATATAGC+AGG - Chr4:67234132-67234151 MsG0480022032.01.T01:intron 40.0%
GTAGTTCAGCATATCACATG+AGG + Chr4:67234308-67234327 None:intergenic 40.0%
GTATCTGTGCAGATATGTGT+TGG - Chr4:67230705-67230724 MsG0480022032.01.T01:three_prime_UTR 40.0%
GTGCAATGGCATATCATTTG+AGG - Chr4:67233929-67233948 MsG0480022032.01.T01:intron 40.0%
GTTCACAGCAACAATGACTT+TGG + Chr4:67234169-67234188 None:intergenic 40.0%
GTTCACATATCCAAGTCTGA+AGG + Chr4:67232598-67232617 None:intergenic 40.0%
GTTCCTTAAACAATGCCCTT+AGG - Chr4:67233084-67233103 MsG0480022032.01.T01:intron 40.0%
TACATCGTCATGATTGGACA+TGG - Chr4:67231105-67231124 MsG0480022032.01.T01:CDS 40.0%
TACTATCCCATTAGCTATGG+AGG + Chr4:67231996-67232015 None:intergenic 40.0%
TCAACATATAGCAGGGATGA+GGG - Chr4:67234140-67234159 MsG0480022032.01.T01:intron 40.0%
TCACATATCCAAGTCTGAAG+GGG + Chr4:67232596-67232615 None:intergenic 40.0%
TCATACAGGCCAATATATGC+AGG - Chr4:67232234-67232253 MsG0480022032.01.T01:intron 40.0%
TCTCCAGTTACTGATGTTAC+AGG - Chr4:67230950-67230969 MsG0480022032.01.T01:intron 40.0%
TGATAAGGCTTCCATATCTC+AGG - Chr4:67232359-67232378 MsG0480022032.01.T01:intron 40.0%
TGCTGATGCTCCTATATTTC+AGG - Chr4:67234194-67234213 MsG0480022032.01.T01:intron 40.0%
TGGAACATTATACTACCGGT+AGG - Chr4:67234527-67234546 MsG0480022032.01.T01:intron 40.0%
TGTGCAGATATGTGTTGGAA+GGG - Chr4:67230710-67230729 MsG0480022032.01.T01:three_prime_UTR 40.0%
TTCAACATATAGCAGGGATG+AGG - Chr4:67234139-67234158 MsG0480022032.01.T01:intron 40.0%
! AACAGAGGGTCTTGTCAAAT+AGG + Chr4:67231175-67231194 None:intergenic 40.0%
! AATGCACGGATTGTAGTTAC+TGG - Chr4:67232726-67232745 MsG0480022032.01.T01:intron 40.0%
! AATGTAAGTTGAGAAAGGGG+TGG - Chr4:67230677-67230696 MsG0480022032.01.T01:three_prime_UTR 40.0%
! ACTGAGCAAATTTTGTGCTG+TGG - Chr4:67231141-67231160 MsG0480022032.01.T01:CDS 40.0%
! ATGAGTTGTGACTTGTGACT+TGG - Chr4:67231763-67231782 MsG0480022032.01.T01:intron 40.0%
! ATTCCAGTTCATTGATGGCA+TGG - Chr4:67232917-67232936 MsG0480022032.01.T01:CDS 40.0%
! ATTTGCCATAGCTAGCTTCT+AGG - Chr4:67231706-67231725 MsG0480022032.01.T01:intron 40.0%
! CTTTTTACATCCCTTGCTAC+TGG - Chr4:67233429-67233448 MsG0480022032.01.T01:intron 40.0%
! GAACTACAGGTTGCTGATTA+TGG - Chr4:67234321-67234340 MsG0480022032.01.T01:CDS 40.0%
! TGAGTTGTGACTTGTGACTT+GGG - Chr4:67231764-67231783 MsG0480022032.01.T01:intron 40.0%
! TTAGGGTAGGTATTGCTATC+AGG - Chr4:67231679-67231698 MsG0480022032.01.T01:intron 40.0%
!! AAATGGACTAGTTTAGCCCA+GGG + Chr4:67230836-67230855 None:intergenic 40.0%
!! ATTAGGTAGCCTTATGCTAG+AGG - Chr4:67233835-67233854 MsG0480022032.01.T01:intron 40.0%
!! GGGGGTTTTGATAGTTTACT+TGG - Chr4:67230745-67230764 MsG0480022032.01.T01:three_prime_UTR 40.0%
!! GTTGCTGATTATGGACTAGT+AGG - Chr4:67234330-67234349 MsG0480022032.01.T01:CDS 40.0%
!! TTCCCTAAGGGCATTGTTTA+AGG + Chr4:67233090-67233109 None:intergenic 40.0%
!!! TTATTGTTTTGGCAGCTGAC+TGG - Chr4:67232107-67232126 MsG0480022032.01.T01:intron 40.0%
AACTGTTTCAGGATTGCTCG+TGG + Chr4:67234282-67234301 None:intergenic 45.0%
ATGATGAAGGACCAGACCTT+GGG - Chr4:67232703-67232722 MsG0480022032.01.T01:intron 45.0%
CAAACTTCTCGGGATGATGA+AGG - Chr4:67232690-67232709 MsG0480022032.01.T01:intron 45.0%
CAAGACCTAGAAGCTAGCTA+TGG + Chr4:67231714-67231733 None:intergenic 45.0%
CACCTCTTTGCTGAACCAAA+TGG + Chr4:67230853-67230872 None:intergenic 45.0%
CATCATATCCAACCCTTCCT+CGG + Chr4:67233804-67233823 None:intergenic 45.0%
CATCTGTGTGTGGAACAAAG+AGG - Chr4:67233571-67233590 MsG0480022032.01.T01:intron 45.0%
CATGCTAGCAAGTTCCCTAA+GGG + Chr4:67233102-67233121 None:intergenic 45.0%
CCGTTTAGACTTTCACAAGC+AGG - Chr4:67231930-67231949 MsG0480022032.01.T01:intron 45.0%
CGCTATCATGTGTTAAGGCA+TGG + Chr4:67233154-67233173 None:intergenic 45.0%
CTCAAACAGTCCAGGTTTAC+AGG + Chr4:67231643-67231662 None:intergenic 45.0%
CTGGGCTAAACTAGTCCATT+TGG - Chr4:67230835-67230854 MsG0480022032.01.T01:exon 45.0%
CTGTAAACCTGGACTGTTTG+AGG - Chr4:67231641-67231660 MsG0480022032.01.T01:intron 45.0%
CTGTGCAGATATGTGTTGGA+AGG - Chr4:67230709-67230728 MsG0480022032.01.T01:three_prime_UTR 45.0%
GACAGTTGCAAAGCAAGAGA+AGG + Chr4:67231277-67231296 None:intergenic 45.0%
GAGTAGCACCAACTGAACAA+AGG + Chr4:67233251-67233270 None:intergenic 45.0%
GGAAAATTAAAACGCCAGCC+TGG - Chr4:67233752-67233771 MsG0480022032.01.T01:intron 45.0%
GTTGCAAAGCAAGAGAAGGA+TGG + Chr4:67231273-67231292 None:intergenic 45.0%
TCATGCTAGCAAGTTCCCTA+AGG + Chr4:67233103-67233122 None:intergenic 45.0%
TCTCCATGCCATCAATGAAC+TGG + Chr4:67232923-67232942 None:intergenic 45.0%
TGACAGATCTGGACAAGTCT+AGG - Chr4:67232658-67232677 MsG0480022032.01.T01:intron 45.0%
TGTTGCTCCCGAACTCTATA+TGG - Chr4:67234095-67234114 MsG0480022032.01.T01:intron 45.0%
! AAGGACTGGCACCAGTATAT+CGG + Chr4:67232278-67232297 None:intergenic 45.0%
! CATTTGGTTCAGCAAAGAGG+TGG - Chr4:67230851-67230870 MsG0480022032.01.T01:CDS 45.0%
! CCCGAACTCTATATGGCTTT+TGG - Chr4:67234102-67234121 MsG0480022032.01.T01:intron 45.0%
! GGAAGCTTTTCCAACAACTC+TGG + Chr4:67234375-67234394 None:intergenic 45.0%
! GTCCATTTGGTTCAGCAAAG+AGG - Chr4:67230848-67230867 MsG0480022032.01.T01:CDS 45.0%
! TCAACAGGCTTCTGAGAAAC+AGG + Chr4:67232330-67232349 None:intergenic 45.0%
! TTACTTGGGTATGCTGCTTG+TGG - Chr4:67230760-67230779 MsG0480022032.01.T01:three_prime_UTR 45.0%
!! CAAATGGACTAGTTTAGCCC+AGG + Chr4:67230837-67230856 None:intergenic 45.0%
!! GAATTATATATTAAAATATA+TGG + Chr4:67233132-67233151 None:intergenic 5.0%
AACGGAGTACACCAGTAGCA+AGG + Chr4:67233443-67233462 None:intergenic 50.0%
ACCAGCAGCACCAAGTTTCT+TGG + Chr4:67232802-67232821 None:intergenic 50.0%
ACGGAGTACACCAGTAGCAA+GGG + Chr4:67233442-67233461 None:intergenic 50.0%
ACTCCCTTTGGACTGTGTCT+GGG - Chr4:67231328-67231347 MsG0480022032.01.T01:intron 50.0%
CTACAATCCGTGCATTCCCA+AGG + Chr4:67232722-67232741 None:intergenic 50.0%
CTGGTCCAATCGCTGTGTAT+GGG - Chr4:67233771-67233790 MsG0480022032.01.T01:intron 50.0%
CTTCCCAGACACAGTCCAAA+GGG + Chr4:67231334-67231353 None:intergenic 50.0%
GATAGGTCCTCAAACAGTCC+AGG + Chr4:67231651-67231670 None:intergenic 50.0%
GATGATGAAGGACCAGACCT+TGG - Chr4:67232702-67232721 MsG0480022032.01.T01:intron 50.0%
TCTAAAAGAGCAGCAGCACG+CGG + Chr4:67230926-67230945 None:intergenic 50.0%
TCTTCCCAGACACAGTCCAA+AGG + Chr4:67231335-67231354 None:intergenic 50.0%
TGAATGACGAGGTCTCACTC+TGG - Chr4:67233400-67233419 MsG0480022032.01.T01:intron 50.0%
TGGTCCAATCGCTGTGTATG+GGG - Chr4:67233772-67233791 MsG0480022032.01.T01:intron 50.0%
! GCACGGATTGTAGTTACTGG+TGG - Chr4:67232729-67232748 MsG0480022032.01.T01:intron 50.0%
! TGTGACTTGTGACTTGGGTG+AGG - Chr4:67231769-67231788 MsG0480022032.01.T01:intron 50.0%
AGAACCCATTGGCAGAACCC+TGG - Chr4:67230816-67230835 MsG0480022032.01.T01:three_prime_UTR 55.0%
ATCCGTGCATTCCCAAGGTC+TGG + Chr4:67232717-67232736 None:intergenic 55.0%
CAATCGCTGTGTATGGGGTC+CGG - Chr4:67233777-67233796 MsG0480022032.01.T01:intron 55.0%
CACTCCCTTTGGACTGTGTC+TGG - Chr4:67231327-67231346 MsG0480022032.01.T01:intron 55.0%
CCATACACAGCGATTGGACC+AGG + Chr4:67233773-67233792 None:intergenic 55.0%
CCTGGTCCAATCGCTGTGTA+TGG - Chr4:67233770-67233789 MsG0480022032.01.T01:intron 55.0%
GAACCCATTGGCAGAACCCT+GGG - Chr4:67230817-67230836 MsG0480022032.01.T01:three_prime_UTR 55.0%
GACCAGACCTTGGGAATGCA+CGG - Chr4:67232712-67232731 MsG0480022032.01.T01:intron 55.0%
TAGCCCAGGGTTCTGCCAAT+GGG + Chr4:67230823-67230842 None:intergenic 55.0%
TATCCAACCCTTCCTCGGAC+CGG + Chr4:67233799-67233818 None:intergenic 55.0%
TTAGCCCAGGGTTCTGCCAA+TGG + Chr4:67230824-67230843 None:intergenic 55.0%
! ACTCGTGCTGCTGTTGATGC+AGG - Chr4:67233267-67233286 MsG0480022032.01.T01:intron 55.0%
! CGAGGTGCTACCAGAGTTGT+TGG - Chr4:67234362-67234381 MsG0480022032.01.T01:CDS 55.0%
! GGGTATGCTGCTTGTGGTGT+AGG - Chr4:67230766-67230785 MsG0480022032.01.T01:three_prime_UTR 55.0%
!! GCCAAGAAACTTGGTGCTGC+TGG - Chr4:67232798-67232817 MsG0480022032.01.T01:intron 55.0%
ACACAGCGATTGGACCAGGC+TGG + Chr4:67233769-67233788 None:intergenic 60.0%
CGGACCCCATACACAGCGAT+TGG + Chr4:67233779-67233798 None:intergenic 60.0%
GCTTGTGGTGTAGGTGCTCG+TGG - Chr4:67230775-67230794 MsG0480022032.01.T01:three_prime_UTR 60.0%
TGTGGTGTAGGTGCTCGTGG+CGG - Chr4:67230778-67230797 MsG0480022032.01.T01:three_prime_UTR 60.0%
ATGGGGTCCGGTCCGAGGAA+GGG - Chr4:67233789-67233808 MsG0480022032.01.T01:intron 65.0%
TATGGGGTCCGGTCCGAGGA+AGG - Chr4:67233788-67233807 MsG0480022032.01.T01:intron 65.0%
TGTGTATGGGGTCCGGTCCG+AGG - Chr4:67233784-67233803 MsG0480022032.01.T01:intron 65.0%
GGTCCGGTCCGAGGAAGGGT+TGG - Chr4:67233793-67233812 MsG0480022032.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 67230656 67234595 67230656 ID=MsG0480022032.01;Name=MsG0480022032.01
Chr4 mRNA 67230656 67234595 67230656 ID=MsG0480022032.01.T01;Parent=MsG0480022032.01;Name=MsG0480022032.01.T01;_AED=0.45;_eAED=0.46;_QI=0|0.83|0.85|1|1|1|7|188|275
Chr4 exon 67230656 67230921 67230656 ID=MsG0480022032.01.T01:exon:8221;Parent=MsG0480022032.01.T01
Chr4 exon 67231036 67231176 67231036 ID=MsG0480022032.01.T01:exon:8220;Parent=MsG0480022032.01.T01
Chr4 exon 67231940 67231992 67231940 ID=MsG0480022032.01.T01:exon:8219;Parent=MsG0480022032.01.T01
Chr4 exon 67232432 67232587 67232432 ID=MsG0480022032.01.T01:exon:8218;Parent=MsG0480022032.01.T01
Chr4 exon 67232846 67233009 67232846 ID=MsG0480022032.01.T01:exon:8217;Parent=MsG0480022032.01.T01
Chr4 exon 67234252 67234463 67234252 ID=MsG0480022032.01.T01:exon:8216;Parent=MsG0480022032.01.T01
Chr4 exon 67234572 67234595 67234572 ID=MsG0480022032.01.T01:exon:8215;Parent=MsG0480022032.01.T01
Chr4 CDS 67234572 67234595 67234572 ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4 CDS 67234252 67234463 67234252 ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4 CDS 67232846 67233009 67232846 ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4 CDS 67232432 67232587 67232432 ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4 CDS 67231940 67231992 67231940 ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4 CDS 67231036 67231176 67231036 ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4 CDS 67230844 67230921 67230844 ID=MsG0480022032.01.T01:cds;Parent=MsG0480022032.01.T01
Chr4 three_prime_UTR 67230656 67230843 67230656 ID=MsG0480022032.01.T01:three_prime_utr;Parent=MsG0480022032.01.T01
Gene Sequence

>MsG0480022032.01.T01

ATGATAAAAAGAGGAAAAGAAAATGTGCTCGTGGCGGATTCAGATACATTCAAGAACCCATTGGCAGAACCCTGGGCTAAACTAGTCCATTTGGTTCAGCAAAGAGGTGGTTACTCTCATATAATTGCAGCTTCAAATTCTTTTGGGAAAAATGTCATGCCGCGTGCTGCTGCTCTTTTAGATGTTTCTCCAGTTACTGATGTTACAGGAATATCTGATTCTCATACTTTTATAAGGCCAATATATGCAGGAAATGCACTTTGTACAGTCCGATATACTGGTGCCAGTCCTTGTATATTAACAATTAGGTCAACTTCTTTTCCTGTTTCTCAGAAGCCTGTTGACTCAAAATCTGATAAGGCTTCCATATCTCAGGTTGATCTTTCAACCTTTGACGAAGATCTGGACAAGTCTAGGTATATATCTCAAACTTCTCGGGATGATGAAGGACCAGACCTTGGGAATGCACGGATTGTAGTTACTGGTGGTCGTGCACTAAAGAGTGCTGAGAACTTTAAGTTGATCGAAAATCTAGCCAAGAAACTTGGTGCTGCTGTTGGTGCTACTCGTGCTGCTGTTGATGCAGGATTTGTTGCTAATGATCTCCAGGTTGGACAAACTGGAAAGATTGTTGCTCCCGAACTCTATATGGCTTTTGGTGTATCTGGAGCTATTCAACATATAGCAGGGATGAGGGATTCCAAAGTCATTGTTGCTGTGAACAACGATGCTGATGCTCCTATATTTCAGGTTGCTGATTATGGACTAGTAGGAGATTTATTCGAGGTGCTACCAGAGTTGTTGGAAAAGCTTCCTGAGAAAAAATAA
Protein sequence

>MsG0480022032.01.T01

MIKRGKENVLVADSDTFKNPLAEPWAKLVHLVQQRGGYSHIIAASNSFGKNVMPRAAALLDVSPVTDVTGISDSHTFIRPIYAGNALCTVRYTGASPCILTIRSTSFPVSQKPVDSKSDKASISQVDLSTFDEDLDKSRYISQTSRDDEGPDLGNARIVVTGGRALKSAENFKLIENLAKKLGAAVGATRAAVDAGFVANDLQVGQTGKIVAPELYMAFGVSGAIQHIAGMRDSKVIVAVNNDADAPIFQVADYGLVGDLFEVLPELLEKLPEKK*