AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022058.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022058.01.T01 MTR_4g081510 65.138 218 34 5 1 218 1 176 2.29e-82 243
MsG0480022058.01.T01 MTR_4g081520 49.682 157 38 5 9 165 15 130 5.68e-29 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022058.01.T01 AT5G54580 40.805 174 61 5 6 179 18 149 5.43e-24 93.6

Find 58 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACCGGTGACGAAGAGAATT+TGG 0.272558 4:+67528967 None:intergenic
GAGGCTATAGCATTGGTTCT+TGG 0.297566 4:+67527254 None:intergenic
AAAGTTCGGTGAAGTTAAAC+AGG 0.321973 4:-67528186 MsG0480022058.01.T01:CDS
TCAGGTTACTCTAAGGGATT+TGG 0.331272 4:-67528007 MsG0480022058.01.T01:CDS
TCGTGTCTCAGGTTACTCTA+AGG 0.355649 4:-67528014 MsG0480022058.01.T01:CDS
TTCATTTCACCGTTCTTCTC+TGG 0.361360 4:+67527179 None:intergenic
GGGATGGTTCAGTATGTGTC+TGG 0.374916 4:+67527231 None:intergenic
CGGTGCCCCGCGGTTTCTGC+CGG 0.374929 4:-67529088 MsG0480022058.01.T01:CDS
AATCATAGCTGAAACTATAA+TGG 0.379515 4:-67528074 MsG0480022058.01.T01:CDS
GAAAAGCCGGCAGAAACCGC+GGG 0.416020 4:+67529082 None:intergenic
CAGTATTGTGTTACATGTGC+AGG 0.434335 4:+67527897 None:intergenic
ACATGTGCAGGCAGGCTAAA+GGG 0.469771 4:+67527909 None:intergenic
GATGCAGCTAAGGGCATTCA+AGG 0.474539 4:-67527959 MsG0480022058.01.T01:CDS
AGAGCAGAAGTAGCTCTAGA+AGG 0.482168 4:+67529008 None:intergenic
AAGGCGGCGTTGAAGCGAAC+GGG 0.486768 4:+67529027 None:intergenic
TACTTCAGAAGATGCAGCTA+AGG 0.490832 4:-67527969 MsG0480022058.01.T01:CDS
GTGGTGGCAGATCGTGTCTC+AGG 0.492116 4:-67528025 MsG0480022058.01.T01:CDS
GATGAGAGCTACAGCGGCAG+CGG 0.497516 4:-67529108 MsG0480022058.01.T01:CDS
TACATGTGCAGGCAGGCTAA+AGG 0.500876 4:+67527908 None:intergenic
TTGGCTTCGTGCAGAAAAGC+CGG 0.502812 4:+67529069 None:intergenic
GTGACGAAGAGAATTTGGTT+GGG 0.517626 4:+67528972 None:intergenic
AACCAAATTCTCTTCGTCAC+CGG 0.521059 4:-67528969 MsG0480022058.01.T01:CDS
TCTTCAGGTTCGGAGTGGGA+TGG 0.524520 4:+67527215 None:intergenic
GCCGATCTCAGCAAGGGTGG+TGG 0.532889 4:-67528041 MsG0480022058.01.T01:CDS
TCCGTTTGCCGATCTCAGCA+AGG 0.532977 4:-67528048 MsG0480022058.01.T01:CDS
ACAGCGGCAGCGGTGCCCCG+CGG 0.533814 4:-67529098 MsG0480022058.01.T01:CDS
CCGTTTGCCGATCTCAGCAA+GGG 0.533857 4:-67528047 MsG0480022058.01.T01:CDS
GGAAACTGCTGGCAAGAAGA+AGG 0.538907 4:-67527153 MsG0480022058.01.T01:CDS
CCCTTGCTGAGATCGGCAAA+CGG 0.539709 4:+67528047 None:intergenic
GGTGACGAAGAGAATTTGGT+TGG 0.541116 4:+67528971 None:intergenic
CGTGTCTCAGGTTACTCTAA+GGG 0.541129 4:-67528013 MsG0480022058.01.T01:CDS
GAAGAACGGTGAAATGAAGA+AGG 0.549581 4:-67527174 MsG0480022058.01.T01:CDS
GGATGGTTCAGTATGTGTCT+GGG 0.554245 4:+67527232 None:intergenic
AAAACGACGCCAGAGAAGAA+CGG 0.554308 4:-67527188 MsG0480022058.01.T01:CDS
GCCACCACCCTTGCTGAGAT+CGG 0.559836 4:+67528040 None:intergenic
GAAGGCGGCGTTGAAGCGAA+CGG 0.562134 4:+67529026 None:intergenic
TGTCTGGGAGGCTATAGCAT+TGG 0.576971 4:+67527247 None:intergenic
CATTCAAGGCATGGATGGCA+AGG 0.577087 4:-67527945 MsG0480022058.01.T01:CDS
AAGGGCATTCAAGGCATGGA+TGG 0.580521 4:-67527950 MsG0480022058.01.T01:CDS
AGAAAAGCCGGCAGAAACCG+CGG 0.588918 4:+67529081 None:intergenic
AGCTAAGGGCATTCAAGGCA+TGG 0.591683 4:-67527954 MsG0480022058.01.T01:CDS
ACTTCAGAAGATGCAGCTAA+GGG 0.597665 4:-67527968 MsG0480022058.01.T01:CDS
AGCTACTTCTGCTCTAAAGA+CGG 0.598572 4:-67529000 MsG0480022058.01.T01:CDS
ATTGTGTTACATGTGCAGGC+AGG 0.606976 4:+67527901 None:intergenic
GTTGAAGCGAACGGGACAGT+GGG 0.608011 4:+67529035 None:intergenic
TTTGCCGATCTCAGCAAGGG+TGG 0.614124 4:-67528044 MsG0480022058.01.T01:CDS
CGTTGAAGCGAACGGGACAG+TGG 0.621721 4:+67529034 None:intergenic
CGGGACAGTGGGAGAAGACG+CGG 0.622686 4:+67529046 None:intergenic
GAAATGAAGAAGGAAACTGC+TGG 0.640066 4:-67527164 MsG0480022058.01.T01:CDS
TGGTTCAGTATGTGTCTGGG+AGG 0.643151 4:+67527235 None:intergenic
AAAAGCCGGCAGAAACCGCG+GGG 0.647049 4:+67529083 None:intergenic
ACAGTGGGAGAAGACGCGGT+TGG 0.653547 4:+67529050 None:intergenic
GTACTACATCAGAGACGCTG+AGG 0.664662 4:-67528219 MsG0480022058.01.T01:CDS
TTTGATGATGAGAGCTACAG+CGG 0.670929 4:-67529114 None:intergenic
TACTACATCAGAGACGCTGA+GGG 0.671573 4:-67528218 MsG0480022058.01.T01:CDS
TACTTCTGCTCTAAAGACGG+CGG 0.680130 4:-67528997 MsG0480022058.01.T01:CDS
GCAGAAGTAGCTCTAGAAGG+CGG 0.685533 4:+67529011 None:intergenic
AAGTTCGGTGAAGTTAAACA+GGG 0.706995 4:-67528185 MsG0480022058.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAGGAGTAAATATTAAT+CGG - Chr4:67527549-67527568 MsG0480022058.01.T01:intron 20.0%
!! CAAATGTTAAAATTTACCAA+GGG + Chr4:67528069-67528088 None:intergenic 20.0%
!! CATAATATTCATTTGATTGT+GGG - Chr4:67527939-67527958 MsG0480022058.01.T01:CDS 20.0%
!! TCAAATGTTAAAATTTACCA+AGG + Chr4:67528070-67528089 None:intergenic 20.0%
!! TCATAATATTCATTTGATTG+TGG - Chr4:67527938-67527957 MsG0480022058.01.T01:CDS 20.0%
!! TTATGAAAGACAAATTGAAT+TGG + Chr4:67527924-67527943 None:intergenic 20.0%
!! TTGTTAATGTAACTGTAAAT+TGG - Chr4:67527298-67527317 MsG0480022058.01.T01:intron 20.0%
!! TTTATGCAATTTGTAACAAA+AGG + Chr4:67527435-67527454 None:intergenic 20.0%
!!! TGTTTTTTTATGTTCAACAA+AGG - Chr4:67527463-67527482 MsG0480022058.01.T01:intron 20.0%
! AATCATAGCTGAAACTATAA+TGG - Chr4:67528142-67528161 MsG0480022058.01.T01:intron 25.0%
! TAAACAAAGTTTATCTTAGG+TGG + Chr4:67528426-67528445 None:intergenic 25.0%
! TGTTTAAAGAGGGAAATTAA+GGG + Chr4:67527800-67527819 None:intergenic 25.0%
! TTAAATGTGTCATAACTTTC+AGG - Chr4:67527964-67527983 MsG0480022058.01.T01:CDS 25.0%
!! CTCTTTTCTTTTTCTTATCA+TGG + Chr4:67527596-67527615 None:intergenic 25.0%
!!! CTTCATTTTTATTTATGGCA+GGG + Chr4:67527869-67527888 None:intergenic 25.0%
!!! GACAGCTTCATTTTTATTTA+TGG + Chr4:67527874-67527893 None:intergenic 25.0%
!!! GTTCTCATTTGATTTGATTT+AGG - Chr4:67527393-67527412 MsG0480022058.01.T01:intron 25.0%
!!! TTCATTTTTATTTATGGCAG+GGG + Chr4:67527868-67527887 None:intergenic 25.0%
AAAGGATCATTTCTTCTTAG+AGG - Chr4:67527481-67527500 MsG0480022058.01.T01:intron 30.0%
AAGAATAGTGAATGCATTGT+TGG + Chr4:67528508-67528527 None:intergenic 30.0%
AATGACAGTTGTAACATGAA+TGG + Chr4:67527825-67527844 None:intergenic 30.0%
AATGCATGATTTACAGATTC+AGG + Chr4:67528681-67528700 None:intergenic 30.0%
AGATCATTGAATTTCATGTC+AGG + Chr4:67528625-67528644 None:intergenic 30.0%
AGTAATAAGCAATTACTGAC+CGG + Chr4:67527269-67527288 None:intergenic 30.0%
AGTAATCTCTCAAACGATTT+GGG - Chr4:67527621-67527640 MsG0480022058.01.T01:intron 30.0%
ATGAATGGTGTGTTTAAAGA+GGG + Chr4:67527810-67527829 None:intergenic 30.0%
ATGCATGATTTACAGATTCA+GGG + Chr4:67528680-67528699 None:intergenic 30.0%
CAGCAATAATTGAATCGAAT+CGG + Chr4:67528878-67528897 None:intergenic 30.0%
CGGTAAACAAAGTTTATCTT+AGG + Chr4:67528429-67528448 None:intergenic 30.0%
GAAAAAGAAGATAACTGCAA+AGG - Chr4:67529090-67529109 MsG0480022058.01.T01:CDS 30.0%
GTGTTTAAAGAGGGAAATTA+AGG + Chr4:67527801-67527820 None:intergenic 30.0%
GTTTAAAGAGGGAAATTAAG+GGG + Chr4:67527799-67527818 None:intergenic 30.0%
TAGTAATCTCTCAAACGATT+TGG - Chr4:67527620-67527639 MsG0480022058.01.T01:intron 30.0%
TCTTAAAGCACGAATTTGTA+TGG - Chr4:67528524-67528543 MsG0480022058.01.T01:intron 30.0%
TTTCTTATCATGGCAAATGT+TGG + Chr4:67527586-67527605 None:intergenic 30.0%
! TGCATCTTTTTACATGACAA+CGG - Chr4:67528723-67528742 MsG0480022058.01.T01:intron 30.0%
!! ACATTGTTGTTGTTTATGTC+TGG - Chr4:67528783-67528802 MsG0480022058.01.T01:intron 30.0%
!! ACTGATTTGAGTTTATGACT+TGG - Chr4:67527516-67527535 MsG0480022058.01.T01:intron 30.0%
!! GCTTCATTTTTATTTATGGC+AGG + Chr4:67527870-67527889 None:intergenic 30.0%
!! TGATTTAGGTTGTCTTGTTA+CGG - Chr4:67527407-67527426 MsG0480022058.01.T01:intron 30.0%
!!! TCGTATTTCATGTTTTGATC+AGG - Chr4:67527344-67527363 MsG0480022058.01.T01:intron 30.0%
AGAAAAGCAAAGAACAACCA+TGG + Chr4:67528845-67528864 None:intergenic 35.0%
AGTGTGATGTGTATATGTTG+CGG + Chr4:67528455-67528474 None:intergenic 35.0%
CATGAATGGTGTGTTTAAAG+AGG + Chr4:67527811-67527830 None:intergenic 35.0%
CTGTCAGAATATTGTTGTCT+TGG - Chr4:67528352-67528371 MsG0480022058.01.T01:intron 35.0%
GGTTGAGATCAAAATAGCTA+GGG + Chr4:67527740-67527759 None:intergenic 35.0%
GTAATCTCTCAAACGATTTG+GGG - Chr4:67527622-67527641 MsG0480022058.01.T01:intron 35.0%
TAAATAGCTGAACGAGTGAA+TGG + Chr4:67528094-67528113 None:intergenic 35.0%
TCATTGAATTTCATGTCAGG+AGG + Chr4:67528622-67528641 None:intergenic 35.0%
TTTACTGCAAACTCGAATAG+AGG + Chr4:67528551-67528570 None:intergenic 35.0%
! AAAGTTCGGTGAAGTTAAAC+AGG - Chr4:67528030-67528049 MsG0480022058.01.T01:CDS 35.0%
! AAGTTCGGTGAAGTTAAACA+GGG - Chr4:67528031-67528050 MsG0480022058.01.T01:CDS 35.0%
! AGCTGTCTATTGCATTGATA+AGG - Chr4:67527887-67527906 MsG0480022058.01.T01:intron 35.0%
! TAATCTCTCAAACGATTTGG+GGG - Chr4:67527623-67527642 MsG0480022058.01.T01:intron 35.0%
!! ACAACTCACTCTAGTCTTTT+GGG + Chr4:67527692-67527711 None:intergenic 35.0%
!! CGTTTTCTTTTCTTCAGGTT+CGG + Chr4:67529014-67529033 None:intergenic 35.0%
!! CTTCTAAAACTGCATTGCAA+AGG + Chr4:67528914-67528933 None:intergenic 35.0%
!! GAGGGAAGCATTAAAAAGTT+CGG - Chr4:67528016-67528035 MsG0480022058.01.T01:CDS 35.0%
!! GATTTAGGTTGTCTTGTTAC+GGG - Chr4:67527408-67527427 MsG0480022058.01.T01:intron 35.0%
!!! ATGTTTTGATCAGGTTGTTG+AGG - Chr4:67527353-67527372 MsG0480022058.01.T01:intron 35.0%
!!! CTTTGCAATGCAGTTTTAGA+AGG - Chr4:67528912-67528931 MsG0480022058.01.T01:intron 35.0%
AAATTAAGGGGAACGCTGTT+TGG + Chr4:67527787-67527806 None:intergenic 40.0%
AACCAAATTCTCTTCGTCAC+CGG - Chr4:67527247-67527266 MsG0480022058.01.T01:CDS 40.0%
AATTTCATGTCAGGAGGAAG+GGG + Chr4:67528616-67528635 None:intergenic 40.0%
ACTTCAGAAGATGCAGCTAA+GGG - Chr4:67528248-67528267 MsG0480022058.01.T01:intron 40.0%
ACTTGGTTCAATCTCGACAA+AGG - Chr4:67527533-67527552 MsG0480022058.01.T01:intron 40.0%
ATCTCAACCCACAATTTGCA+TGG - Chr4:67527751-67527770 MsG0480022058.01.T01:intron 40.0%
ATGTGTATATGTTGCGGTGT+CGG + Chr4:67528449-67528468 None:intergenic 40.0%
CAGTATTGTGTTACATGTGC+AGG + Chr4:67528322-67528341 None:intergenic 40.0%
GAAATGAAGAAGGAAACTGC+TGG - Chr4:67529052-67529071 MsG0480022058.01.T01:CDS 40.0%
GAAGAACGGTGAAATGAAGA+AGG - Chr4:67529042-67529061 MsG0480022058.01.T01:CDS 40.0%
GAATTTCATGTCAGGAGGAA+GGG + Chr4:67528617-67528636 None:intergenic 40.0%
GGGTTGAGATCAAAATAGCT+AGG + Chr4:67527741-67527760 None:intergenic 40.0%
GTGACGAAGAGAATTTGGTT+GGG + Chr4:67527247-67527266 None:intergenic 40.0%
TACTTCAGAAGATGCAGCTA+AGG - Chr4:67528247-67528266 MsG0480022058.01.T01:intron 40.0%
TGAATTTCATGTCAGGAGGA+AGG + Chr4:67528618-67528637 None:intergenic 40.0%
TTCATTTCACCGTTCTTCTC+TGG + Chr4:67529040-67529059 None:intergenic 40.0%
TTTATTTATGGCAGGGGAAG+AGG + Chr4:67527862-67527881 None:intergenic 40.0%
! TCAGGTTACTCTAAGGGATT+TGG - Chr4:67528209-67528228 MsG0480022058.01.T01:CDS 40.0%
!! AGCTACTTCTGCTCTAAAGA+CGG - Chr4:67527216-67527235 MsG0480022058.01.T01:CDS 40.0%
!! AGCTCAACTGGTTTGAGATA+AGG - Chr4:67527652-67527671 MsG0480022058.01.T01:intron 40.0%
!! CACAACTCACTCTAGTCTTT+TGG + Chr4:67527693-67527712 None:intergenic 40.0%
!! CATCTACAAAGTTGATGCCA+TGG - Chr4:67528825-67528844 MsG0480022058.01.T01:intron 40.0%
!! GCTCAACTGGTTTGAGATAA+GGG - Chr4:67527653-67527672 MsG0480022058.01.T01:intron 40.0%
AAAACGACGCCAGAGAAGAA+CGG - Chr4:67529028-67529047 MsG0480022058.01.T01:CDS 45.0%
AACCCACAATTTGCATGGTG+TGG - Chr4:67527756-67527775 MsG0480022058.01.T01:intron 45.0%
AGGGTATGTTCTTCATCCCT+TGG - Chr4:67528050-67528069 MsG0480022058.01.T01:CDS 45.0%
ATTGTGTTACATGTGCAGGC+AGG + Chr4:67528318-67528337 None:intergenic 45.0%
ATTTCATGTCAGGAGGAAGG+GGG + Chr4:67528615-67528634 None:intergenic 45.0%
CACCACACCATGCAAATTGT+GGG + Chr4:67527761-67527780 None:intergenic 45.0%
CGTGTCTCAGGTTACTCTAA+GGG - Chr4:67528203-67528222 MsG0480022058.01.T01:CDS 45.0%
GGATGGTTCAGTATGTGTCT+GGG + Chr4:67528987-67529006 None:intergenic 45.0%
GGTGACGAAGAGAATTTGGT+TGG + Chr4:67527248-67527267 None:intergenic 45.0%
TACTACATCAGAGACGCTGA+GGG - Chr4:67527998-67528017 MsG0480022058.01.T01:CDS 45.0%
TCACCACACCATGCAAATTG+TGG + Chr4:67527762-67527781 None:intergenic 45.0%
TCGTGTCTCAGGTTACTCTA+AGG - Chr4:67528202-67528221 MsG0480022058.01.T01:CDS 45.0%
! TCTCAAACGATTTGGGGGTT+TGG - Chr4:67527628-67527647 MsG0480022058.01.T01:intron 45.0%
!! AGAGCAGAAGTAGCTCTAGA+AGG + Chr4:67527211-67527230 None:intergenic 45.0%
!! CTTTTCTTCAGGTTCGGAGT+GGG + Chr4:67529008-67529027 None:intergenic 45.0%
!! GAGGCTATAGCATTGGTTCT+TGG + Chr4:67528965-67528984 None:intergenic 45.0%
!! GGCGTCGTTTTCTTTTCTTC+AGG + Chr4:67529019-67529038 None:intergenic 45.0%
!! TACTTCTGCTCTAAAGACGG+CGG - Chr4:67527219-67527238 MsG0480022058.01.T01:CDS 45.0%
!! TCTTTTCTTCAGGTTCGGAG+TGG + Chr4:67529009-67529028 None:intergenic 45.0%
!!! GCAATGCAGTTTTAGAAGGC+TGG - Chr4:67528916-67528935 MsG0480022058.01.T01:intron 45.0%
ACATGTGCAGGCAGGCTAAA+GGG + Chr4:67528310-67528329 None:intergenic 50.0%
CATTCAAGGCATGGATGGCA+AGG - Chr4:67528271-67528290 MsG0480022058.01.T01:intron 50.0%
GACCGGTGACGAAGAGAATT+TGG + Chr4:67527252-67527271 None:intergenic 50.0%
GGAAACTGCTGGCAAGAAGA+AGG - Chr4:67529063-67529082 MsG0480022058.01.T01:CDS 50.0%
GGGATGGTTCAGTATGTGTC+TGG + Chr4:67528988-67529007 None:intergenic 50.0%
GTACTACATCAGAGACGCTG+AGG - Chr4:67527997-67528016 MsG0480022058.01.T01:CDS 50.0%
TACATGTGCAGGCAGGCTAA+AGG + Chr4:67528311-67528330 None:intergenic 50.0%
TGGTTCAGTATGTGTCTGGG+AGG + Chr4:67528984-67529003 None:intergenic 50.0%
TGTCTGGGAGGCTATAGCAT+TGG + Chr4:67528972-67528991 None:intergenic 50.0%
TTGGCTTCGTGCAGAAAAGC+CGG + Chr4:67527150-67527169 None:intergenic 50.0%
TTTCATGTCAGGAGGAAGGG+GGG + Chr4:67528614-67528633 None:intergenic 50.0%
!! AAGGGCATTCAAGGCATGGA+TGG - Chr4:67528266-67528285 MsG0480022058.01.T01:intron 50.0%
!! AGCTAAGGGCATTCAAGGCA+TGG - Chr4:67528262-67528281 MsG0480022058.01.T01:intron 50.0%
!! GATGCAGCTAAGGGCATTCA+AGG - Chr4:67528257-67528276 MsG0480022058.01.T01:intron 50.0%
!! GCAGAAGTAGCTCTAGAAGG+CGG + Chr4:67527208-67527227 None:intergenic 50.0%
AGAAAAGCCGGCAGAAACCG+CGG + Chr4:67527138-67527157 None:intergenic 55.0%
CCCTTGCTGAGATCGGCAAA+CGG + Chr4:67528172-67528191 None:intergenic 55.0%
CCGTTTGCCGATCTCAGCAA+GGG - Chr4:67528169-67528188 MsG0480022058.01.T01:intron 55.0%
GTTGAAGCGAACGGGACAGT+GGG + Chr4:67527184-67527203 None:intergenic 55.0%
TCCGTTTGCCGATCTCAGCA+AGG - Chr4:67528168-67528187 MsG0480022058.01.T01:intron 55.0%
TTCATGTCAGGAGGAAGGGG+GGG + Chr4:67528613-67528632 None:intergenic 55.0%
TTTGCCGATCTCAGCAAGGG+TGG - Chr4:67528172-67528191 MsG0480022058.01.T01:intron 55.0%
! TCTTCAGGTTCGGAGTGGGA+TGG + Chr4:67529004-67529023 None:intergenic 55.0%
! TGGGGGTTTGGTAGCTCAAC+TGG - Chr4:67527640-67527659 MsG0480022058.01.T01:intron 55.0%
AAAAGCCGGCAGAAACCGCG+GGG + Chr4:67527136-67527155 None:intergenic 60.0%
ACAGTGGGAGAAGACGCGGT+TGG + Chr4:67527169-67527188 None:intergenic 60.0%
CGTTGAAGCGAACGGGACAG+TGG + Chr4:67527185-67527204 None:intergenic 60.0%
GAAAAGCCGGCAGAAACCGC+GGG + Chr4:67527137-67527156 None:intergenic 60.0%
GCCACCACCCTTGCTGAGAT+CGG + Chr4:67528179-67528198 None:intergenic 60.0%
GTGGTGGCAGATCGTGTCTC+AGG - Chr4:67528191-67528210 MsG0480022058.01.T01:CDS 60.0%
! AAGGCGGCGTTGAAGCGAAC+GGG + Chr4:67527192-67527211 None:intergenic 60.0%
! GAAGGCGGCGTTGAAGCGAA+CGG + Chr4:67527193-67527212 None:intergenic 60.0%
CGGGACAGTGGGAGAAGACG+CGG + Chr4:67527173-67527192 None:intergenic 65.0%
GCCGATCTCAGCAAGGGTGG+TGG - Chr4:67528175-67528194 MsG0480022058.01.T01:intron 65.0%
CGGTGCCCCGCGGTTTCTGC+CGG - Chr4:67527128-67527147 MsG0480022058.01.T01:CDS 75.0%
! ACAGCGGCAGCGGTGCCCCG+CGG - Chr4:67527118-67527137 MsG0480022058.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr4 gene 67527109 67529129 67527109 ID=MsG0480022058.01;Name=MsG0480022058.01
Chr4 mRNA 67527109 67529129 67527109 ID=MsG0480022058.01.T01;Parent=MsG0480022058.01;Name=MsG0480022058.01.T01;_AED=0.48;_eAED=0.54;_QI=0|0|0|1|1|0.75|4|0|218
Chr4 exon 67528966 67529129 67528966 ID=MsG0480022058.01.T01:exon:8335;Parent=MsG0480022058.01.T01
Chr4 exon 67528186 67528252 67528186 ID=MsG0480022058.01.T01:exon:8334;Parent=MsG0480022058.01.T01
Chr4 exon 67527911 67528131 67527911 ID=MsG0480022058.01.T01:exon:8333;Parent=MsG0480022058.01.T01
Chr4 exon 67527109 67527313 67527109 ID=MsG0480022058.01.T01:exon:8332;Parent=MsG0480022058.01.T01
Chr4 CDS 67528966 67529129 67528966 ID=MsG0480022058.01.T01:cds;Parent=MsG0480022058.01.T01
Chr4 CDS 67528186 67528252 67528186 ID=MsG0480022058.01.T01:cds;Parent=MsG0480022058.01.T01
Chr4 CDS 67527911 67528131 67527911 ID=MsG0480022058.01.T01:cds;Parent=MsG0480022058.01.T01
Chr4 CDS 67527109 67527313 67527109 ID=MsG0480022058.01.T01:cds;Parent=MsG0480022058.01.T01
Gene Sequence

>MsG0480022058.01.T01

ATGAGAGCTACAGCGGCAGCGGTGCCCCGCGGTTTCTGCCGGCTTTTCTGCACGAAGCCAACCGCGTCTTCTCCCACTGTCCCGTTCGCTTCAACGCCGCCTTCTAGAGCTACTTCTGCTCTAAAGACGGCGGAGCCCAACCAAATTCTCTTCGTCACCGGTCAGCTTAACAAACGTACTACATCAGAGACGCTGAGGGAAGCATTAAAAAGTTCGGTGAAGTTAAACAGGCTATTTATGCTTTTACTATGCGATGATGTCTTAAAAATCATAGCTGAAACTATAATGGTTTTCCGTTTGCCGATCTCAGCAAGGGTGGTGGCAGATCGTGTCTCAGGTTACTCTAAGGGATTTGGTTACGTGAAATATGCTACTTCAGAAGATGCAGCTAAGGGCATTCAAGGCATGGATGGCAAGGTCAAATTTATTAACCCTTTAGCCTGCCTGCACATTTTTAGAAGGCTGGATTGTATTGCAGAGTATGCAAGACCAAGAACCAATGCTATAGCCTCCCAGACACATACTGAACCATCCCACTCCGAACCTGAAGAAAAGAAAACGACGCCAGAGAAGAACGGTGAAATGAAGAAGGAAACTGCTGGCAAGAAGAAGGACAAGAAAAAGAAGATAACTGCAAAGGTAGTTGCAGACGAATGA
Protein sequence

>MsG0480022058.01.T01

MRATAAAVPRGFCRLFCTKPTASSPTVPFASTPPSRATSALKTAEPNQILFVTGQLNKRTTSETLREALKSSVKLNRLFMLLLCDDVLKIIAETIMVFRLPISARVVADRVSGYSKGFGYVKYATSEDAAKGIQGMDGKVKFINPLACLHIFRRLDCIAEYARPRTNAIASQTHTEPSHSEPEEKKTTPEKNGEMKKETAGKKKDKKKKITAKVVADE*