AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022059.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022059.01.T01 MTR_4g081520 78.710 155 8 2 15 169 15 144 3.15e-65 196
MsG0480022059.01.T01 MTR_4g081510 57.333 150 37 3 6 155 1 123 3.27e-47 152
MsG0480022059.01.T01 MTR_7g099940 53.333 75 30 1 57 126 36 110 1.72e-19 80.1
MsG0480022059.01.T01 MTR_6g477870 46.341 82 44 0 40 121 79 160 2.16e-18 77.8
MsG0480022059.01.T01 MTR_3g025880 45.783 83 37 1 54 128 42 124 5.90e-18 80.5
MsG0480022059.01.T01 MTR_6g477870 46.914 81 43 0 40 120 79 159 1.01e-17 76.6
MsG0480022059.01.T01 MTR_4g109540 42.683 82 42 1 44 120 25 106 4.64e-17 76.3
MsG0480022059.01.T01 MTR_3g084040 40.909 88 49 2 37 124 19 103 7.28e-16 69.7
MsG0480022059.01.T01 MTR_7g105260 45.205 73 35 1 58 125 34 106 9.23e-16 70.5
MsG0480022059.01.T01 MTR_7g013740 46.269 67 36 0 54 120 38 104 8.97e-15 68.2
MsG0480022059.01.T01 MTR_4g109540 40.000 90 41 2 44 120 25 114 1.17e-14 70.1
MsG0480022059.01.T01 MTR_3g084040 41.667 84 46 2 37 120 19 99 1.33e-14 67.4
MsG0480022059.01.T01 MTR_1g092860 40.000 75 45 0 47 121 183 257 5.16e-14 68.2
MsG0480022059.01.T01 MTR_7g013730 43.939 66 37 0 55 120 39 104 5.88e-14 67.8
MsG0480022059.01.T01 MTR_4g070080 40.000 70 42 0 51 120 1 70 4.49e-13 63.2
MsG0480022059.01.T01 MTR_4g070080 40.000 70 42 0 51 120 1 70 9.84e-13 62.8
MsG0480022059.01.T01 MTR_3g029580 43.056 72 41 0 55 126 31 102 1.22e-12 62.0
MsG0480022059.01.T01 MTR_4g070190 41.935 62 36 0 59 120 11 72 1.50e-12 62.4
MsG0480022059.01.T01 MTR_4g036415 40.000 85 51 0 35 119 430 514 2.02e-12 64.7
MsG0480022059.01.T01 MTR_3g080130 41.975 81 45 2 56 135 36 115 3.13e-12 60.8
MsG0480022059.01.T01 MTR_7g117495 41.667 60 35 0 56 115 18 77 8.61e-12 62.0
MsG0480022059.01.T01 MTR_7g117495 41.667 60 35 0 56 115 18 77 9.02e-12 62.0
MsG0480022059.01.T01 MTR_8g024520 43.478 69 35 1 56 120 25 93 1.64e-11 61.2
MsG0480022059.01.T01 MTR_8g024520 43.478 69 35 1 56 120 25 93 1.66e-11 61.2
MsG0480022059.01.T01 MTR_4g070140 43.548 62 35 0 59 120 11 72 3.32e-11 59.3
MsG0480022059.01.T01 MTR_3g029580 41.791 67 39 0 55 121 31 97 3.60e-11 58.2
MsG0480022059.01.T01 MTR_3g116130 45.614 57 31 0 56 112 29 85 7.61e-11 59.3
MsG0480022059.01.T01 MTR_6g040070 40.845 71 41 1 57 127 210 279 8.98e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022059.01.T01 AT5G54580 50.000 168 56 3 3 168 9 150 8.00e-42 137
MsG0480022059.01.T01 AT2G37510 57.534 73 26 1 58 125 36 108 4.34e-20 81.6
MsG0480022059.01.T01 AT1G73530 50.746 67 33 0 54 120 75 141 2.47e-18 78.2
MsG0480022059.01.T01 AT3G23830 44.048 84 44 1 37 120 19 99 2.06e-14 66.6
MsG0480022059.01.T01 AT3G23830 44.048 84 44 1 37 120 19 99 2.06e-14 66.6
MsG0480022059.01.T01 AT2G37510 42.857 98 26 2 58 125 36 133 2.43e-14 67.4
MsG0480022059.01.T01 AT4G13850 50.000 66 33 0 55 120 34 99 2.90e-14 66.6
MsG0480022059.01.T01 AT4G13850 50.000 66 33 0 55 120 34 99 3.01e-14 66.2
MsG0480022059.01.T01 AT1G18630 44.737 76 37 1 56 126 36 111 3.26e-14 66.6
MsG0480022059.01.T01 AT4G13850 50.000 66 33 0 55 120 34 99 3.51e-14 66.6
MsG0480022059.01.T01 AT4G13850 50.000 66 33 0 55 120 34 99 3.89e-14 66.6
MsG0480022059.01.T01 AT5G61030 44.776 67 37 0 54 120 38 104 1.21e-13 67.4
MsG0480022059.01.T01 AT5G61030 44.776 67 37 0 54 120 38 104 1.21e-13 67.4
MsG0480022059.01.T01 AT5G06210 42.105 76 39 1 55 125 33 108 1.52e-13 64.7
MsG0480022059.01.T01 AT4G13860 46.970 66 35 0 55 120 2 67 8.11e-13 61.2
MsG0480022059.01.T01 AT3G08000 39.506 81 49 0 57 137 42 122 8.53e-13 62.8
MsG0480022059.01.T01 AT3G20930 41.791 67 39 0 55 121 281 347 9.50e-13 65.5
MsG0480022059.01.T01 AT2G46780 44.444 63 35 0 56 118 22 84 2.08e-12 64.3
MsG0480022059.01.T01 AT2G46780 44.444 63 35 0 56 118 22 84 2.08e-12 64.3
MsG0480022059.01.T01 AT5G51300 35.714 98 58 2 22 119 451 543 2.46e-12 64.3
MsG0480022059.01.T01 AT5G51300 35.714 98 58 2 22 119 451 543 2.46e-12 64.3
MsG0480022059.01.T01 AT5G51300 35.714 98 58 2 22 119 451 543 2.46e-12 64.3
MsG0480022059.01.T01 AT3G46020 40.299 67 40 0 55 121 6 72 5.78e-12 59.3
MsG0480022059.01.T01 AT4G39260 40.000 70 42 0 51 120 1 70 7.65e-12 58.9
MsG0480022059.01.T01 AT4G39260 40.000 70 42 0 51 120 1 70 1.05e-11 59.3
MsG0480022059.01.T01 AT2G43370 33.684 95 61 1 44 138 52 144 1.08e-11 60.8
MsG0480022059.01.T01 AT1G74230 43.284 67 37 1 54 120 32 97 1.34e-11 61.6
MsG0480022059.01.T01 AT4G39260 40.000 70 42 0 51 120 1 70 2.40e-11 59.3
MsG0480022059.01.T01 AT5G53720 41.558 77 38 2 51 120 1 77 4.42e-11 57.0
MsG0480022059.01.T01 AT5G47320 39.506 81 46 1 40 120 18 95 5.76e-11 59.3
MsG0480022059.01.T01 AT1G22910 47.273 55 29 0 56 110 13 67 6.77e-11 59.3
MsG0480022059.01.T01 AT1G22910 47.273 55 29 0 56 110 13 67 7.18e-11 59.3
MsG0480022059.01.T01 AT2G43370 33.684 95 61 1 44 138 52 144 7.96e-11 59.7
MsG0480022059.01.T01 AT1G22330 31.429 105 46 2 56 156 17 99 8.74e-11 59.3

Find 82 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATGCCTGGAACCTTTAT+TGG 0.155390 4:-67531424 MsG0480022059.01.T01:three_prime_UTR
TTCTCATCATCGCCGCCTTC+TGG 0.178462 4:-67534274 MsG0480022059.01.T01:CDS
GAACAACTTCACCGAACTTT+TGG 0.218023 4:+67532775 None:intergenic
GCAGTAACCATTACCAATAA+AGG 0.306069 4:+67531411 None:intergenic
TCAGGTTACTCTAAGGGATT+TGG 0.331272 4:-67532607 MsG0480022059.01.T01:CDS
CCTGGAACCTTTATTGGTAA+TGG 0.341065 4:-67531418 MsG0480022059.01.T01:three_prime_UTR
AGTATTATTTATTTAGTCAT+TGG 0.346573 4:-67531548 MsG0480022059.01.T01:three_prime_UTR
TCGAGTCTCAGGTTACTCTA+AGG 0.347313 4:-67532614 MsG0480022059.01.T01:CDS
CACTTGAGTCTTCTCGTATA+AGG 0.355217 4:+67532492 None:intergenic
CGGATGAACATAAATTAATA+AGG 0.358256 4:+67531613 None:intergenic
GCTTCTCCGGGTGGTGGTCT+TGG 0.365700 4:+67531655 None:intergenic
CGTATTATTTACAGCTTCTC+CGG 0.378906 4:+67531642 None:intergenic
GAGAGCTGCAGCTGCGGCGG+CGG 0.386601 4:-67534362 MsG0480022059.01.T01:CDS
AAGTACATTAAAACCGCATT+TGG 0.389834 4:-67534482 MsG0480022059.01.T01:five_prime_UTR
AAGTTCGGTGAAGTTGTTCA+TGG 0.397028 4:-67532771 MsG0480022059.01.T01:intron
CTCTCTCCTTCAACCAATCA+AGG 0.399507 4:-67534431 MsG0480022059.01.T01:five_prime_UTR
TGAAAAGTAAAACCCTTGAT+TGG 0.411509 4:+67534418 None:intergenic
CCATTACCAATAAAGGTTCC+AGG 0.427934 4:+67531418 None:intergenic
AGCACCAACCTCTTCGTCTC+TGG 0.450848 4:-67534217 MsG0480022059.01.T01:intron
CGGAGAAGCTGTAAATAATA+CGG 0.453875 4:-67531641 MsG0480022059.01.T01:CDS
GATGCTGCTAGGGGCATTGA+AGG 0.455789 4:-67532559 MsG0480022059.01.T01:CDS
GATTATCCTCCCATTTCAAC+GGG 0.459573 4:+67531481 None:intergenic
TTGCAAATTCAAACATAATG+AGG 0.470183 4:+67531578 None:intergenic
TAGATAAATTAAGGCTAGCT+AGG 0.473341 4:+67531514 None:intergenic
AAAGAGTCGGCAGAAACTGC+GGG 0.478130 4:+67534336 None:intergenic
TACTTTAGAAGATGCTGCTA+GGG 0.485381 4:-67532569 MsG0480022059.01.T01:CDS
TAAAACCCTTGATTGGTTGA+AGG 0.489607 4:+67534425 None:intergenic
AGGGAAGCATTCCAAAAGTT+CGG 0.494589 4:-67532786 MsG0480022059.01.T01:CDS
AATATTTCTCTTGATATGCC+TGG 0.498184 4:-67531436 MsG0480022059.01.T01:three_prime_UTR
TATAAGGTGCAGGCAGGCAA+AGG 0.498752 4:+67532508 None:intergenic
ATCTGTCGAGCGGAAGGCGG+CGG 0.498886 4:+67534250 None:intergenic
TTTACAGCTTCTCCGGGTGG+TGG 0.499800 4:+67531649 None:intergenic
CGTCAAAGCAGCCATGGGAT+GGG 0.502131 4:+67534387 None:intergenic
CTACTTTAGAAGATGCTGCT+AGG 0.502306 4:-67532570 MsG0480022059.01.T01:CDS
GTGGTAACAGATCGAGTCTC+AGG 0.506975 4:-67532625 MsG0480022059.01.T01:CDS
GCCGCCTTCCGCTCGACAGA+TGG 0.507477 4:-67534248 MsG0480022059.01.T01:CDS
GATGAGAGCTGCAGCTGCGG+CGG 0.510313 4:-67534365 MsG0480022059.01.T01:CDS
TCGTCAAAGCAGCCATGGGA+TGG 0.510354 4:+67534386 None:intergenic
TGGAGAAGAAGCGGTTGGAT+TGG 0.515749 4:+67534309 None:intergenic
GATGAGAAAGAAGGGAAAGC+TGG 0.519875 4:+67534289 None:intergenic
TATTTATTTAGTCATTGGCA+TGG 0.524751 4:-67531543 MsG0480022059.01.T01:three_prime_UTR
GGCGGCGATGATGAGAAAGA+AGG 0.531889 4:+67534280 None:intergenic
TTCTCGTATAAGGTGCAGGC+AGG 0.532816 4:+67532502 None:intergenic
AGTCTTCTCGTATAAGGTGC+AGG 0.536420 4:+67532498 None:intergenic
CGAGTCTCAGGTTACTCTAA+GGG 0.537803 4:-67532613 MsG0480022059.01.T01:CDS
GTCAAAGCAGCCATGGGATG+GGG 0.541430 4:+67534388 None:intergenic
GAAGAGGTTGGTGCTAGGCT+CGG 0.548156 4:+67534225 None:intergenic
GTATTATTTACAGCTTCTCC+GGG 0.550728 4:+67531643 None:intergenic
TCTCATCGTCAAAGCAGCCA+TGG 0.558291 4:+67534381 None:intergenic
TCTCTCCTTCAACCAATCAA+GGG 0.558703 4:-67534430 MsG0480022059.01.T01:five_prime_UTR
GCGGAAGGCGGCGGTCCAGA+AGG 0.560349 4:+67534259 None:intergenic
GAAGGCGGCGGTCCAGAAGG+CGG 0.560594 4:+67534262 None:intergenic
TCGGCCATCTGTCGAGCGGA+AGG 0.561453 4:+67534244 None:intergenic
ATAAGGTGCAGGCAGGCAAA+GGG 0.561846 4:+67532509 None:intergenic
GACGATGAGAGCTGCAGCTG+CGG 0.562915 4:-67534368 MsG0480022059.01.T01:CDS
TCAGCTTAAACCCCATCCCA+TGG 0.564938 4:-67534398 MsG0480022059.01.T01:exon
AGATTATCCTCCCATTTCAA+CGG 0.572042 4:+67531480 None:intergenic
GCCATCTGTCGAGCGGAAGG+CGG 0.576022 4:+67534247 None:intergenic
TGAACATAAATTAATAAGGT+CGG 0.576922 4:+67531617 None:intergenic
TGCTAGGGGCATTGAAGGAA+TGG 0.581040 4:-67532554 MsG0480022059.01.T01:CDS
CTCATCGTCAAAGCAGCCAT+GGG 0.581556 4:+67534382 None:intergenic
GCGGCGATGATGAGAAAGAA+GGG 0.588249 4:+67534281 None:intergenic
GGAGAAGCTGTAAATAATAC+GGG 0.589568 4:-67531640 MsG0480022059.01.T01:CDS
AAAGCTGGAGAAGAAGCGGT+TGG 0.590957 4:+67534304 None:intergenic
GAGACGAAGAGGTTGGTGCT+AGG 0.593582 4:+67534220 None:intergenic
CGAACCAGAGACGAAGAGGT+TGG 0.593995 4:+67534213 None:intergenic
AGGGGCATTGAAGGAATGGA+TGG 0.598515 4:-67532550 MsG0480022059.01.T01:CDS
AGGGAAAGCTGGAGAAGAAG+CGG 0.611069 4:+67534300 None:intergenic
TTGGATTGGTGCAAAAGAGT+CGG 0.611688 4:+67534323 None:intergenic
AAAAGAGTCGGCAGAAACTG+CGG 0.620147 4:+67534335 None:intergenic
TTATTTACAGCTTCTCCGGG+TGG 0.622983 4:+67531646 None:intergenic
CATTGAAGGAATGGATGGCA+AGG 0.640049 4:-67532545 MsG0480022059.01.T01:CDS
AAGGCGAACCAGAGACGAAG+AGG 0.640180 4:+67534209 None:intergenic
ATATCTGAACTCGCCAAATG+CGG 0.642153 4:+67534469 None:intergenic
TTTGTCAATCTCAGCAAGGG+TGG 0.647632 4:-67532644 MsG0480022059.01.T01:intron
GTACTACAACAGAGACGCTG+AGG 0.653561 4:-67532806 MsG0480022059.01.T01:CDS
AGGCTCGGCCATCTGTCGAG+CGG 0.658207 4:+67534240 None:intergenic
GCAAGACCAAGACCACCACC+CGG 0.665523 4:-67531661 MsG0480022059.01.T01:CDS
TACTACAACAGAGACGCTGA+GGG 0.682055 4:-67532805 MsG0480022059.01.T01:CDS
ACTTTAGAAGATGCTGCTAG+GGG 0.684689 4:-67532568 MsG0480022059.01.T01:CDS
AATTTGCCCGTTGAAATGGG+AGG 0.697791 4:-67531487 MsG0480022059.01.T01:three_prime_UTR
AAGAGTCGGCAGAAACTGCG+GGG 0.717765 4:+67534337 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATAATAATAATAATA+AGG + Chr4:67531709-67531728 None:intergenic 0.0%
!!! AAATTATTTATTTTTATTTA+TGG + Chr4:67532942-67532961 None:intergenic 0.0%
!! ATTATAATTATTATTGTTGT+TGG - Chr4:67531719-67531738 MsG0480022059.01.T01:intron 10.0%
!!! AAATTTTTGTAGATAAATTA+AGG + Chr4:67534374-67534393 None:intergenic 10.0%
!! AGTATTATTTATTTAGTCAT+TGG - Chr4:67534328-67534347 MsG0480022059.01.T01:CDS 15.0%
!!! ATTTATTTTTATTTATGGCA+TGG + Chr4:67532937-67532956 None:intergenic 15.0%
!!! CTTTTTTATAATCCTTAAAA+CGG + Chr4:67531885-67531904 None:intergenic 15.0%
!!! TTTATTTTTATTTATGGCAT+GGG + Chr4:67532936-67532955 None:intergenic 15.0%
!! AGTTTAAATGTTTAAAAGGA+TGG + Chr4:67533139-67533158 None:intergenic 20.0%
!! CAAAAAACATAAAACCATTA+TGG + Chr4:67533216-67533235 None:intergenic 20.0%
!! GAACAGTTTAAATGTTTAAA+AGG + Chr4:67533143-67533162 None:intergenic 20.0%
!! TATTATAAAGACATACAATG+AGG + Chr4:67533633-67533652 None:intergenic 20.0%
!! TGAACATAAATTAATAAGGT+CGG + Chr4:67534262-67534281 None:intergenic 20.0%
!! TGTTTAAGCAATAAGTAAAT+AGG + Chr4:67532087-67532106 None:intergenic 20.0%
!! TTTATCAATTTCATCATAGA+AGG - Chr4:67532314-67532333 MsG0480022059.01.T01:intron 20.0%
!!! ACAAATTGGTTTTGTAAATA+AGG - Chr4:67532345-67532364 MsG0480022059.01.T01:intron 20.0%
!!! ACATTGTTATTGTATATGTA+TGG - Chr4:67534046-67534065 MsG0480022059.01.T01:intron 20.0%
!!! TTACAAAACCAATTTGTTAA+AGG + Chr4:67532342-67532361 None:intergenic 20.0%
! AAAATTGCAAATGACAATTG+AGG + Chr4:67532903-67532922 None:intergenic 25.0%
! AAATTAAGAGAATTGAATCC+TGG + Chr4:67532853-67532872 None:intergenic 25.0%
! AATACATGATTTACAGATTC+GGG + Chr4:67533929-67533948 None:intergenic 25.0%
! AATTAAGAGAATTGAATCCT+GGG + Chr4:67532852-67532871 None:intergenic 25.0%
! ACGATATAAGTAGAAATAAG+AGG - Chr4:67532455-67532474 MsG0480022059.01.T01:intron 25.0%
! AGAAAATATACTGTATGAGT+TGG - Chr4:67532393-67532412 MsG0480022059.01.T01:intron 25.0%
! AGATCATTAAATTTGATGTC+AGG + Chr4:67533872-67533891 None:intergenic 25.0%
! ATAATAAGGAAAAGTTGAGA+AGG + Chr4:67531695-67531714 None:intergenic 25.0%
! ATTTAATACACTTAATTCCC+AGG - Chr4:67532832-67532851 MsG0480022059.01.T01:intron 25.0%
! CAATACATGATTTACAGATT+CGG + Chr4:67533930-67533949 None:intergenic 25.0%
! CAATATTTGAATAGGAAGTT+TGG + Chr4:67534133-67534152 None:intergenic 25.0%
! CGATATAAGTAGAAATAAGA+GGG - Chr4:67532456-67532475 MsG0480022059.01.T01:intron 25.0%
! CGGATGAACATAAATTAATA+AGG + Chr4:67534266-67534285 None:intergenic 25.0%
! GATATAAGTAGAAATAAGAG+GGG - Chr4:67532457-67532476 MsG0480022059.01.T01:intron 25.0%
! GTAAATTAAGTTATCTTAGG+TGG + Chr4:67533675-67533694 None:intergenic 25.0%
! TAATAAGGAAAAGTTGAGAA+GGG + Chr4:67531694-67531713 None:intergenic 25.0%
! TACTAATATCAAACCACAAA+GGG + Chr4:67532170-67532189 None:intergenic 25.0%
! TATTTATTTAGTCATTGGCA+TGG - Chr4:67534333-67534352 MsG0480022059.01.T01:CDS 25.0%
! TCAGTAAGCAATATTTGAAT+AGG + Chr4:67534141-67534160 None:intergenic 25.0%
! TCGGTAAATTAAGTTATCTT+AGG + Chr4:67533678-67533697 None:intergenic 25.0%
! TCTTAAAGCACAAATTTGTA+TGG - Chr4:67533772-67533791 MsG0480022059.01.T01:intron 25.0%
! TTACTAATATCAAACCACAA+AGG + Chr4:67532171-67532190 None:intergenic 25.0%
! TTGCAAATTCAAACATAATG+AGG + Chr4:67534301-67534320 None:intergenic 25.0%
! TTTATGTTCTCATTTGATCT+TGG - Chr4:67531843-67531862 MsG0480022059.01.T01:intron 25.0%
!! ACATAAAACCATTATGGTTT+TGG + Chr4:67533210-67533229 None:intergenic 25.0%
!! ATAAATTTCTTTTCCCTTTG+TGG - Chr4:67532154-67532173 MsG0480022059.01.T01:intron 25.0%
!! TTCAAAGAATATGTTTCTGT+TGG + Chr4:67532665-67532684 None:intergenic 25.0%
AAAAATTTGCCCGTTGAAAT+GGG - Chr4:67534386-67534405 MsG0480022059.01.T01:exon 30.0%
AAGAACAGTGAATACATTGT+TGG + Chr4:67533756-67533775 None:intergenic 30.0%
AAGGATCATTTCATAATCCT+CGG - Chr4:67532578-67532597 MsG0480022059.01.T01:CDS 30.0%
AAGTACATTAAAACCGCATT+TGG - Chr4:67531394-67531413 MsG0480022059.01.T01:three_prime_UTR 30.0%
AATATTTCTCTTGATATGCC+TGG - Chr4:67534440-67534459 MsG0480022059.01.T01:five_prime_UTR 30.0%
AATCTACTCCAAAACCATAA+TGG - Chr4:67533199-67533218 MsG0480022059.01.T01:intron 30.0%
AGAAAAGCAAAGAACAATCA+TGG + Chr4:67534108-67534127 None:intergenic 30.0%
ATACATGATTTACAGATTCG+GGG + Chr4:67533928-67533947 None:intergenic 30.0%
GGGAAAAGAAATTTATACAC+AGG + Chr4:67532150-67532169 None:intergenic 30.0%
TAATGAGGCCAAAAATGTTT+CGG + Chr4:67534286-67534305 None:intergenic 30.0%
TCATTAAATTTGATGTCAGG+AGG + Chr4:67533869-67533888 None:intergenic 30.0%
TGAAAAGTAAAACCCTTGAT+TGG + Chr4:67531461-67531480 None:intergenic 30.0%
! ATGCAATTTAGAGCAAGTAT+GGG + Chr4:67532764-67532783 None:intergenic 30.0%
! ATGTTCATCCGAAACATTTT+TGG - Chr4:67534275-67534294 MsG0480022059.01.T01:CDS 30.0%
! TAGATAAATTAAGGCTAGCT+AGG + Chr4:67534365-67534384 None:intergenic 30.0%
! TTGTTATCTTGGATCTGTTT+TGG - Chr4:67533423-67533442 MsG0480022059.01.T01:intron 30.0%
!! TTTTATTTATGGCATGGGAA+GGG + Chr4:67532931-67532950 None:intergenic 30.0%
!! TTTTTATTTATGGCATGGGA+AGG + Chr4:67532932-67532951 None:intergenic 30.0%
!!! TTTATGTTTTGACCATGTTG+AGG - Chr4:67531808-67531827 MsG0480022059.01.T01:intron 30.0%
!!! TTTTTTGTCAATCTCAGCAA+GGG - Chr4:67533229-67533248 MsG0480022059.01.T01:intron 30.0%
AAATTTGATGTCAGGAGGAA+GGG + Chr4:67533864-67533883 None:intergenic 35.0%
AGAAGGAGCCTTTAACAAAT+TGG - Chr4:67532331-67532350 MsG0480022059.01.T01:intron 35.0%
AGATTATCCTCCCATTTCAA+CGG + Chr4:67534399-67534418 None:intergenic 35.0%
ATGCAACTGACAACAAAACT+CGG + Chr4:67534003-67534022 None:intergenic 35.0%
CAAAAATTTGCCCGTTGAAA+TGG - Chr4:67534385-67534404 MsG0480022059.01.T01:exon 35.0%
CATCATATGAAACCTCAACA+TGG + Chr4:67531823-67531842 None:intergenic 35.0%
CGGAGAAGCTGTAAATAATA+CGG - Chr4:67534235-67534254 MsG0480022059.01.T01:CDS 35.0%
CGTATTATTTACAGCTTCTC+CGG + Chr4:67534237-67534256 None:intergenic 35.0%
CTCATTTGATCTTGGTTGTT+TGG - Chr4:67531851-67531870 MsG0480022059.01.T01:intron 35.0%
CTCCAAAAACTGTATTGCAA+GGG + Chr4:67534172-67534191 None:intergenic 35.0%
GACACAGTCAAGAACTTAAA+AGG + Chr4:67532490-67532509 None:intergenic 35.0%
GCAGTAACCATTACCAATAA+AGG + Chr4:67534468-67534487 None:intergenic 35.0%
GGAGAAGCTGTAAATAATAC+GGG - Chr4:67534236-67534255 MsG0480022059.01.T01:CDS 35.0%
GTAAATCTATCTCACTCTCA+CGG + Chr4:67532258-67532277 None:intergenic 35.0%
GTATTATTTACAGCTTCTCC+GGG + Chr4:67534236-67534255 None:intergenic 35.0%
TAAATCTATCTCACTCTCAC+GGG + Chr4:67532257-67532276 None:intergenic 35.0%
TAAATTTGATGTCAGGAGGA+AGG + Chr4:67533865-67533884 None:intergenic 35.0%
TGACGTGTCTTAAACTTTCA+GGG - Chr4:67533037-67533056 MsG0480022059.01.T01:intron 35.0%
TGATCTTGGTTGTTTGGTTA+TGG - Chr4:67531857-67531876 MsG0480022059.01.T01:intron 35.0%
TTGACGTGTCTTAAACTTTC+AGG - Chr4:67533036-67533055 MsG0480022059.01.T01:intron 35.0%
! AATGACAATTGAGGCATCAA+TGG + Chr4:67532894-67532913 None:intergenic 35.0%
! AGACTCAAGTGTATTTCTGT+CGG - Chr4:67533396-67533415 MsG0480022059.01.T01:intron 35.0%
! CATACTTGCTCTAAATTGCA+TGG - Chr4:67532763-67532782 MsG0480022059.01.T01:intron 35.0%
! CATGCAATTTAGAGCAAGTA+TGG + Chr4:67532765-67532784 None:intergenic 35.0%
! TAAAACCCTTGATTGGTTGA+AGG + Chr4:67531454-67531473 None:intergenic 35.0%
! TACTTTAGAAGATGCTGCTA+GGG - Chr4:67533307-67533326 MsG0480022059.01.T01:intron 35.0%
! TTCCCTTGCAATACAGTTTT+TGG - Chr4:67534167-67534186 MsG0480022059.01.T01:intron 35.0%
! TTGGTTATGGATCCGTTTTA+AGG - Chr4:67531870-67531889 MsG0480022059.01.T01:intron 35.0%
!! CTAGAGAAAGCATCTAAGAA+AGG - Chr4:67532559-67532578 MsG0480022059.01.T01:CDS 35.0%
!! CTTGCAATACAGTTTTTGGA+GGG - Chr4:67534171-67534190 MsG0480022059.01.T01:intron 35.0%
!! TTTTTCGCTTTTGAGCCAAA+CGG + Chr4:67532813-67532832 None:intergenic 35.0%
!!! GTTTTTTGTCAATCTCAGCA+AGG - Chr4:67533228-67533247 MsG0480022059.01.T01:intron 35.0%
AACACTTGTGTGTCACATTG+TGG - Chr4:67533558-67533577 MsG0480022059.01.T01:intron 40.0%
AATTTGATGTCAGGAGGAAG+GGG + Chr4:67533863-67533882 None:intergenic 40.0%
ACAATGTGACACACGGATTT+CGG - Chr4:67533508-67533527 MsG0480022059.01.T01:intron 40.0%
AGGAAAAGTTGAGAAGGGAA+AGG + Chr4:67531689-67531708 None:intergenic 40.0%
AGTGTGATGTGTATATGCTG+CGG + Chr4:67533703-67533722 None:intergenic 40.0%
ATATCTGAACTCGCCAAATG+CGG + Chr4:67531410-67531429 None:intergenic 40.0%
ATTTCACTTGTGTGTCATCG+TGG - Chr4:67533457-67533476 MsG0480022059.01.T01:intron 40.0%
CACTTGAGTCTTCTCGTATA+AGG + Chr4:67533387-67533406 None:intergenic 40.0%
CCATTACCAATAAAGGTTCC+AGG + Chr4:67534461-67534480 None:intergenic 40.0%
CCTCCAAAAACTGTATTGCA+AGG + Chr4:67534173-67534192 None:intergenic 40.0%
CCTGGAACCTTTATTGGTAA+TGG - Chr4:67534458-67534477 MsG0480022059.01.T01:five_prime_UTR 40.0%
CTCAAAAGAGACCTACAGTA+CGG - Chr4:67532638-67532657 MsG0480022059.01.T01:intron 40.0%
CTGTCGGAACATTGTTATCT+TGG - Chr4:67533412-67533431 MsG0480022059.01.T01:intron 40.0%
GATATGCCTGGAACCTTTAT+TGG - Chr4:67534452-67534471 MsG0480022059.01.T01:five_prime_UTR 40.0%
GATTATCCTCCCATTTCAAC+GGG + Chr4:67534398-67534417 None:intergenic 40.0%
TCTCACGGGTAGTAAAACTA+AGG + Chr4:67532243-67532262 None:intergenic 40.0%
TCTCTCCTTCAACCAATCAA+GGG - Chr4:67531446-67531465 MsG0480022059.01.T01:three_prime_UTR 40.0%
TTTACTGCAAACTCGAGTAG+AGG + Chr4:67533799-67533818 None:intergenic 40.0%
! AAGTTCGGTGAAGTTGTTCA+TGG - Chr4:67533105-67533124 MsG0480022059.01.T01:intron 40.0%
! ACTTTAGAAGATGCTGCTAG+GGG - Chr4:67533308-67533327 MsG0480022059.01.T01:intron 40.0%
! ATTTCGGTCTTGTGAAAGAG+AGG - Chr4:67533524-67533543 MsG0480022059.01.T01:intron 40.0%
! CAATGGCATGTTGTTTAGAG+AGG + Chr4:67532877-67532896 None:intergenic 40.0%
! CTACTTTAGAAGATGCTGCT+AGG - Chr4:67533306-67533325 MsG0480022059.01.T01:intron 40.0%
! GAACAACTTCACCGAACTTT+TGG + Chr4:67533104-67533123 None:intergenic 40.0%
! TCAGGTTACTCTAAGGGATT+TGG - Chr4:67533269-67533288 MsG0480022059.01.T01:intron 40.0%
! TTTCGGTCTTGTGAAAGAGA+GGG - Chr4:67533525-67533544 MsG0480022059.01.T01:intron 40.0%
!! AATACAGTTTTTGGAGGGCT+GGG - Chr4:67534176-67534195 MsG0480022059.01.T01:intron 40.0%
!! AGGGAAGCATTCCAAAAGTT+CGG - Chr4:67533090-67533109 MsG0480022059.01.T01:intron 40.0%
!! CCTTGCAATACAGTTTTTGG+AGG - Chr4:67534170-67534189 MsG0480022059.01.T01:intron 40.0%
!! TTGGATTGGTGCAAAAGAGT+CGG + Chr4:67531556-67531575 None:intergenic 40.0%
AAAAGAGTCGGCAGAAACTG+CGG + Chr4:67531544-67531563 None:intergenic 45.0%
AATTTGCCCGTTGAAATGGG+AGG - Chr4:67534389-67534408 MsG0480022059.01.T01:exon 45.0%
AGTCTTCTCGTATAAGGTGC+AGG + Chr4:67533381-67533400 None:intergenic 45.0%
ATCCCACTTGTGTGACATTG+TGG + Chr4:67533610-67533629 None:intergenic 45.0%
ATGTGTATATGCTGCGGTGT+CGG + Chr4:67533697-67533716 None:intergenic 45.0%
CACCACAATGTCACACAAGT+GGG - Chr4:67533605-67533624 MsG0480022059.01.T01:intron 45.0%
CACTTGTGTGACATTGTGGT+GGG + Chr4:67533606-67533625 None:intergenic 45.0%
CATTGAAGGAATGGATGGCA+AGG - Chr4:67533331-67533350 MsG0480022059.01.T01:intron 45.0%
CGAGTCTCAGGTTACTCTAA+GGG - Chr4:67533263-67533282 MsG0480022059.01.T01:intron 45.0%
CTCTCTCCTTCAACCAATCA+AGG - Chr4:67531445-67531464 MsG0480022059.01.T01:three_prime_UTR 45.0%
CTTGTGTGTCACATTGTGGT+TGG - Chr4:67533562-67533581 MsG0480022059.01.T01:intron 45.0%
GAAATCCGTGTGTCACATTG+TGG + Chr4:67533509-67533528 None:intergenic 45.0%
GATGAGAAAGAAGGGAAAGC+TGG + Chr4:67531590-67531609 None:intergenic 45.0%
TACTACAACAGAGACGCTGA+GGG - Chr4:67533071-67533090 MsG0480022059.01.T01:intron 45.0%
TATTTATGGCATGGGAAGGG+AGG + Chr4:67532928-67532947 None:intergenic 45.0%
TCGAGTCTCAGGTTACTCTA+AGG - Chr4:67533262-67533281 MsG0480022059.01.T01:intron 45.0%
TTATTTACAGCTTCTCCGGG+TGG + Chr4:67534233-67534252 None:intergenic 45.0%
TTTCTGTTGGACCGTACTGT+AGG + Chr4:67532652-67532671 None:intergenic 45.0%
TTTGTCAATCTCAGCAAGGG+TGG - Chr4:67533232-67533251 MsG0480022059.01.T01:intron 45.0%
! ATTGTGGTGGGAATGTCACA+TGG + Chr4:67533594-67533613 None:intergenic 45.0%
!! CAATACAGTTTTTGGAGGGC+TGG - Chr4:67534175-67534194 MsG0480022059.01.T01:intron 45.0%
!! TTCAAAGCACTGTGACCGTT+TGG - Chr4:67532795-67532814 MsG0480022059.01.T01:CDS 45.0%
AAAGAGTCGGCAGAAACTGC+GGG + Chr4:67531543-67531562 None:intergenic 50.0%
AAAGCTGGAGAAGAAGCGGT+TGG + Chr4:67531575-67531594 None:intergenic 50.0%
ACTTGTGTGTCATCGTGGTG+GGG - Chr4:67533462-67533481 MsG0480022059.01.T01:intron 50.0%
AGGGAAAGCTGGAGAAGAAG+CGG + Chr4:67531579-67531598 None:intergenic 50.0%
ATAAGGTGCAGGCAGGCAAA+GGG + Chr4:67533370-67533389 None:intergenic 50.0%
CACTTGTGTGTCATCGTGGT+GGG - Chr4:67533461-67533480 MsG0480022059.01.T01:intron 50.0%
CCACCACAATGTCACACAAG+TGG - Chr4:67533604-67533623 MsG0480022059.01.T01:intron 50.0%
CCACTTGTGTGACATTGTGG+TGG + Chr4:67533607-67533626 None:intergenic 50.0%
CCCAACCACAATGTGACACA+CGG - Chr4:67533501-67533520 MsG0480022059.01.T01:intron 50.0%
CCGTGTGTCACATTGTGGTT+GGG + Chr4:67533504-67533523 None:intergenic 50.0%
CTCATCGTCAAAGCAGCCAT+GGG + Chr4:67531497-67531516 None:intergenic 50.0%
GCGGCGATGATGAGAAAGAA+GGG + Chr4:67531598-67531617 None:intergenic 50.0%
GTACTACAACAGAGACGCTG+AGG - Chr4:67533070-67533089 MsG0480022059.01.T01:intron 50.0%
GTGGTAACAGATCGAGTCTC+AGG - Chr4:67533251-67533270 MsG0480022059.01.T01:intron 50.0%
TATAAGGTGCAGGCAGGCAA+AGG + Chr4:67533371-67533390 None:intergenic 50.0%
TCACTTGTGTGTCATCGTGG+TGG - Chr4:67533460-67533479 MsG0480022059.01.T01:intron 50.0%
TCAGCTTAAACCCCATCCCA+TGG - Chr4:67531478-67531497 MsG0480022059.01.T01:three_prime_UTR 50.0%
TCCGTGTGTCACATTGTGGT+TGG + Chr4:67533505-67533524 None:intergenic 50.0%
TCTCATCGTCAAAGCAGCCA+TGG + Chr4:67531498-67531517 None:intergenic 50.0%
TGGAGAAGAAGCGGTTGGAT+TGG + Chr4:67531570-67531589 None:intergenic 50.0%
TTCGAGTAGACACTTGACCG+AGG + Chr4:67532598-67532617 None:intergenic 50.0%
TTCTCGTATAAGGTGCAGGC+AGG + Chr4:67533377-67533396 None:intergenic 50.0%
!! AGGGGCATTGAAGGAATGGA+TGG - Chr4:67533326-67533345 MsG0480022059.01.T01:intron 50.0%
!! TGCTAGGGGCATTGAAGGAA+TGG - Chr4:67533322-67533341 MsG0480022059.01.T01:intron 50.0%
AAGAGTCGGCAGAAACTGCG+GGG + Chr4:67531542-67531561 None:intergenic 55.0%
AAGGCGAACCAGAGACGAAG+AGG + Chr4:67531670-67531689 None:intergenic 55.0%
AGCACCAACCTCTTCGTCTC+TGG - Chr4:67531659-67531678 MsG0480022059.01.T01:CDS 55.0%
CGAACCAGAGACGAAGAGGT+TGG + Chr4:67531666-67531685 None:intergenic 55.0%
CGTCAAAGCAGCCATGGGAT+GGG + Chr4:67531492-67531511 None:intergenic 55.0%
GGCGGCGATGATGAGAAAGA+AGG + Chr4:67531599-67531618 None:intergenic 55.0%
GTCAAAGCAGCCATGGGATG+GGG + Chr4:67531491-67531510 None:intergenic 55.0%
TCGTCAAAGCAGCCATGGGA+TGG + Chr4:67531493-67531512 None:intergenic 55.0%
TTCTCATCATCGCCGCCTTC+TGG - Chr4:67531602-67531621 MsG0480022059.01.T01:three_prime_UTR 55.0%
TTTACAGCTTCTCCGGGTGG+TGG + Chr4:67534230-67534249 None:intergenic 55.0%
! CATCGTGGTGGGGATTTCAC+AGG - Chr4:67533472-67533491 MsG0480022059.01.T01:intron 55.0%
!! GAAGAGGTTGGTGCTAGGCT+CGG + Chr4:67531654-67531673 None:intergenic 55.0%
!! GAGACGAAGAGGTTGGTGCT+AGG + Chr4:67531659-67531678 None:intergenic 55.0%
!! GATGCTGCTAGGGGCATTGA+AGG - Chr4:67533317-67533336 MsG0480022059.01.T01:intron 55.0%
GACGATGAGAGCTGCAGCTG+CGG - Chr4:67531508-67531527 MsG0480022059.01.T01:three_prime_UTR 60.0%
GCAAGACCAAGACCACCACC+CGG - Chr4:67534215-67534234 MsG0480022059.01.T01:intron 60.0%
AGGCTCGGCCATCTGTCGAG+CGG + Chr4:67531639-67531658 None:intergenic 65.0%
ATCTGTCGAGCGGAAGGCGG+CGG + Chr4:67531629-67531648 None:intergenic 65.0%
GATGAGAGCTGCAGCTGCGG+CGG - Chr4:67531511-67531530 MsG0480022059.01.T01:three_prime_UTR 65.0%
GCCATCTGTCGAGCGGAAGG+CGG + Chr4:67531632-67531651 None:intergenic 65.0%
TCGGCCATCTGTCGAGCGGA+AGG + Chr4:67531635-67531654 None:intergenic 65.0%
! GCTTCTCCGGGTGGTGGTCT+TGG + Chr4:67534224-67534243 None:intergenic 65.0%
GAAGGCGGCGGTCCAGAAGG+CGG + Chr4:67531617-67531636 None:intergenic 70.0%
GCCGCCTTCCGCTCGACAGA+TGG - Chr4:67531628-67531647 MsG0480022059.01.T01:CDS 70.0%
GAGAGCTGCAGCTGCGGCGG+CGG - Chr4:67531514-67531533 MsG0480022059.01.T01:three_prime_UTR 75.0%
GCGGAAGGCGGCGGTCCAGA+AGG + Chr4:67531620-67531639 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 67531394 67534504 67531394 ID=MsG0480022059.01;Name=MsG0480022059.01
Chr4 mRNA 67531394 67534504 67531394 ID=MsG0480022059.01.T01;Parent=MsG0480022059.01;Name=MsG0480022059.01.T01;_AED=0.44;_eAED=0.48;_QI=103|0.66|0.75|1|0.66|0.5|4|233|169
Chr4 exon 67531394 67531715 67531394 ID=MsG0480022059.01.T01:exon:8339;Parent=MsG0480022059.01.T01
Chr4 exon 67532485 67532652 67532485 ID=MsG0480022059.01.T01:exon:8338;Parent=MsG0480022059.01.T01
Chr4 exon 67532772 67532840 67532772 ID=MsG0480022059.01.T01:exon:8337;Parent=MsG0480022059.01.T01
Chr4 exon 67534218 67534504 67534218 ID=MsG0480022059.01.T01:exon:8336;Parent=MsG0480022059.01.T01
Chr4 five_prime_UTR 67534402 67534504 67534402 ID=MsG0480022059.01.T01:five_prime_utr;Parent=MsG0480022059.01.T01
Chr4 CDS 67534218 67534401 67534218 ID=MsG0480022059.01.T01:cds;Parent=MsG0480022059.01.T01
Chr4 CDS 67532772 67532840 67532772 ID=MsG0480022059.01.T01:cds;Parent=MsG0480022059.01.T01
Chr4 CDS 67532485 67532652 67532485 ID=MsG0480022059.01.T01:cds;Parent=MsG0480022059.01.T01
Chr4 CDS 67531627 67531715 67531627 ID=MsG0480022059.01.T01:cds;Parent=MsG0480022059.01.T01
Chr4 three_prime_UTR 67531394 67531626 67531394 ID=MsG0480022059.01.T01:three_prime_utr;Parent=MsG0480022059.01.T01
Gene Sequence

>MsG0480022059.01.T01

ATGGCTGCTTTGACGATGAGAGCTGCAGCTGCGGCGGCGGTGCCCCGCAGTTTCTGCCGACTCTTTTGCACCAATCCAACCGCTTCTTCTCCAGCTTTCCCTTCTTTCTCATCATCGCCGCCTTCTGGACCGCCGCCTTCCGCTCGACAGATGGCCGAGCCTAGCACCAACCTCTTCGTCTCTGGGCTTAGTAAACGTACTACAACAGAGACGCTGAGGGAAGCATTCCAAAAGTTCGGTGAAGTTGTTCATGCAAGGGTGGTAACAGATCGAGTCTCAGGTTACTCTAAGGGATTTGGTTTCGTGAAATATGCTACTTTAGAAGATGCTGCTAGGGGCATTGAAGGAATGGATGGCAAGGTAAGATTTATTAACCCTTTGCCTGCCTGCACCTTATACGAGAAGACTCAAGTGTATTTCTTTTTTGGAGGGCTGGGTTATATTGCAGAGTATGCAAGACCAAGACCACCACCCGGAGAAGCTGTAAATAATACGGGCCGACCTTATTAA
Protein sequence

>MsG0480022059.01.T01

MAALTMRAAAAAAVPRSFCRLFCTNPTASSPAFPSFSSSPPSGPPPSARQMAEPSTNLFVSGLSKRTTTETLREAFQKFGEVVHARVVTDRVSGYSKGFGFVKYATLEDAARGIEGMDGKVRFINPLPACTLYEKTQVYFFFGGLGYIAEYARPRPPPGEAVNNTGRPY*