Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022096.01.T01 | PNX80164.1 | 69.565 | 115 | 33 | 2 | 12 | 124 | 172 | 286 | 5.47E-44 | 155 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022096.01.T01 | A0A2K3LNP9 | 69.565 | 115 | 33 | 2 | 12 | 124 | 172 | 286 | 2.61e-44 | 155 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480022096.01 | MsG0480022117.01 | 0.829700 | 4.446229e-55 | 4.585943e-52 |
| MsG0480022096.01 | MsG0680030656.01 | 0.804602 | 1.997012e-49 | 1.034007e-46 |
| MsG0480022096.01 | MsG0780038113.01 | 0.817933 | 2.550232e-52 | 1.882583e-49 |
| MsG0280008073.01 | MsG0480022096.01 | 0.809355 | 1.971424e-50 | 1.154988e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022096.01.T01 | MTR_4g082355 | 63.934 | 122 | 43 | 1 | 4 | 124 | 163 | 284 | 2.43e-47 | 154 |
| MsG0480022096.01.T01 | MTR_4g082360 | 69.841 | 126 | 36 | 2 | 1 | 124 | 155 | 280 | 6.80e-41 | 137 |
| MsG0480022096.01.T01 | MTR_4g082365 | 49.138 | 116 | 57 | 2 | 4 | 117 | 140 | 255 | 4.91e-29 | 106 |
| MsG0480022096.01.T01 | MTR_4g082550 | 37.795 | 127 | 58 | 5 | 2 | 108 | 185 | 310 | 2.69e-14 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022096.01.T01 | AT3G11810 | 36.429 | 140 | 63 | 6 | 1 | 114 | 187 | 326 | 1.62e-15 | 71.6 |
Find 30 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTCCCTCTTGTGGTTTATT+TGG | 0.171116 | 4:+68154391 | MsG0480022096.01.T01:CDS |
| GAAGTTGAAGAAGTGGAATT+TGG | 0.233912 | 4:+68154683 | MsG0480022096.01.T01:CDS |
| TCTAGTATATTATGGAATTA+AGG | 0.245448 | 4:-68154732 | None:intergenic |
| AGTTTCCAGCTTCTCCTTAA+TGG | 0.257266 | 4:-68154473 | None:intergenic |
| GTTGGGGTTTGGAACCATTA+AGG | 0.318251 | 4:+68154459 | MsG0480022096.01.T01:CDS |
| CATCGATTAGTGGTTATAGT+TGG | 0.378825 | 4:+68154561 | MsG0480022096.01.T01:CDS |
| AAGTTGAAGAAGTGGAATTT+GGG | 0.381890 | 4:+68154684 | MsG0480022096.01.T01:CDS |
| GGTGGAATCACGTTGGGGTT+TGG | 0.387654 | 4:+68154448 | MsG0480022096.01.T01:CDS |
| ATTAAGGAGAAGCTGGAAAC+TGG | 0.427521 | 4:+68154475 | MsG0480022096.01.T01:CDS |
| TGTAGTGGTGGAATCACGTT+GGG | 0.429158 | 4:+68154442 | MsG0480022096.01.T01:CDS |
| ATCGATTAGTGGTTATAGTT+GGG | 0.432064 | 4:+68154562 | MsG0480022096.01.T01:CDS |
| ACATGATGAAGTTGAAGAAG+TGG | 0.477972 | 4:+68154676 | MsG0480022096.01.T01:CDS |
| GGGATGTTGACATCGATTAG+TGG | 0.493411 | 4:+68154551 | MsG0480022096.01.T01:CDS |
| TGGAACCATTAAGGAGAAGC+TGG | 0.500836 | 4:+68154468 | MsG0480022096.01.T01:CDS |
| GATTCTTATAGTCCCTCTTG+TGG | 0.509438 | 4:+68154382 | MsG0480022096.01.T01:CDS |
| AGTGAAAGCACCGCAAGTAT+AGG | 0.515408 | 4:-68154605 | None:intergenic |
| CGTGGTTTCTCCTATACTTG+CGG | 0.518316 | 4:+68154595 | MsG0480022096.01.T01:CDS |
| AGTGGAATTTGGGAAGGAAA+AGG | 0.544513 | 4:+68154694 | MsG0480022096.01.T01:CDS |
| TTTGGACGTTTACATGATGT+TGG | 0.575084 | 4:+68154409 | MsG0480022096.01.T01:CDS |
| TGAAGAAGTGGAATTTGGGA+AGG | 0.577294 | 4:+68154688 | MsG0480022096.01.T01:CDS |
| ACGTCCAAATAAACCACAAG+AGG | 0.588543 | 4:-68154395 | None:intergenic |
| AACTGGTGAAAGGAATGAAA+AGG | 0.591857 | 4:+68154492 | MsG0480022096.01.T01:CDS |
| GGTGAAAGGAATGAAAAGGT+TGG | 0.594891 | 4:+68154496 | MsG0480022096.01.T01:CDS |
| CGTCCAAATAAACCACAAGA+GGG | 0.623274 | 4:-68154394 | None:intergenic |
| AGAAGCTGGAAACTGGTGAA+AGG | 0.623879 | 4:+68154482 | MsG0480022096.01.T01:CDS |
| GGTGAAAGTGATTGTAGTGG+TGG | 0.643735 | 4:+68154430 | MsG0480022096.01.T01:CDS |
| TTGTAGTGGTGGAATCACGT+TGG | 0.659689 | 4:+68154441 | MsG0480022096.01.T01:CDS |
| ATTTGGGAAGGAAAAGGACG+AGG | 0.663443 | 4:+68154700 | MsG0480022096.01.T01:CDS |
| GTAGTGGTGGAATCACGTTG+GGG | 0.688303 | 4:+68154443 | MsG0480022096.01.T01:CDS |
| GTTGGTGAAAGTGATTGTAG+TGG | 0.711987 | 4:+68154427 | MsG0480022096.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGTTGAAGAAGTGGAATTT+GGG | + | Chr4:68154684-68154703 | MsG0480022096.01.T01:CDS | 30.0% | |
| ATCGATTAGTGGTTATAGTT+GGG | + | Chr4:68154562-68154581 | MsG0480022096.01.T01:CDS | 30.0% | |
| AACTGGTGAAAGGAATGAAA+AGG | + | Chr4:68154492-68154511 | MsG0480022096.01.T01:CDS | 35.0% | |
| ACATGATGAAGTTGAAGAAG+TGG | + | Chr4:68154676-68154695 | MsG0480022096.01.T01:CDS | 35.0% | |
| GAAGTTGAAGAAGTGGAATT+TGG | + | Chr4:68154683-68154702 | MsG0480022096.01.T01:CDS | 35.0% | |
| TTTGGACGTTTACATGATGT+TGG | + | Chr4:68154409-68154428 | MsG0480022096.01.T01:CDS | 35.0% | |
| ! | CATCGATTAGTGGTTATAGT+TGG | + | Chr4:68154561-68154580 | MsG0480022096.01.T01:CDS | 35.0% |
| ! | TAGTTGGGTTATGATTTTCG+TGG | + | Chr4:68154577-68154596 | MsG0480022096.01.T01:CDS | 35.0% |
| !!! | GGCTTTTTCAACCTTGTTTT+TGG | + | Chr4:68154517-68154536 | MsG0480022096.01.T01:CDS | 35.0% |
| ACGTCCAAATAAACCACAAG+AGG | - | Chr4:68154398-68154417 | None:intergenic | 40.0% | |
| AGTCCCTCTTGTGGTTTATT+TGG | + | Chr4:68154391-68154410 | MsG0480022096.01.T01:CDS | 40.0% | |
| AGTGGAATTTGGGAAGGAAA+AGG | + | Chr4:68154694-68154713 | MsG0480022096.01.T01:CDS | 40.0% | |
| AGTTTCCAGCTTCTCCTTAA+TGG | - | Chr4:68154476-68154495 | None:intergenic | 40.0% | |
| ATTAAGGAGAAGCTGGAAAC+TGG | + | Chr4:68154475-68154494 | MsG0480022096.01.T01:CDS | 40.0% | |
| CGTCCAAATAAACCACAAGA+GGG | - | Chr4:68154397-68154416 | None:intergenic | 40.0% | |
| GATTCTTATAGTCCCTCTTG+TGG | + | Chr4:68154382-68154401 | MsG0480022096.01.T01:CDS | 40.0% | |
| GGTGAAAGGAATGAAAAGGT+TGG | + | Chr4:68154496-68154515 | MsG0480022096.01.T01:CDS | 40.0% | |
| TGAAGAAGTGGAATTTGGGA+AGG | + | Chr4:68154688-68154707 | MsG0480022096.01.T01:CDS | 40.0% | |
| TTGCAACGCAACCAAAAACA+AGG | - | Chr4:68154531-68154550 | None:intergenic | 40.0% | |
| ! | GTTGGTGAAAGTGATTGTAG+TGG | + | Chr4:68154427-68154446 | MsG0480022096.01.T01:CDS | 40.0% |
| !!! | TGTTTTTGGTTGCGTTGCAA+GGG | + | Chr4:68154531-68154550 | MsG0480022096.01.T01:CDS | 40.0% |
| !!! | TTGTTTTTGGTTGCGTTGCA+AGG | + | Chr4:68154530-68154549 | MsG0480022096.01.T01:CDS | 40.0% |
| AGAAGCTGGAAACTGGTGAA+AGG | + | Chr4:68154482-68154501 | MsG0480022096.01.T01:CDS | 45.0% | |
| ATTTGGGAAGGAAAAGGACG+AGG | + | Chr4:68154700-68154719 | MsG0480022096.01.T01:CDS | 45.0% | |
| CGTGGTTTCTCCTATACTTG+CGG | + | Chr4:68154595-68154614 | MsG0480022096.01.T01:CDS | 45.0% | |
| GGTGAAAGTGATTGTAGTGG+TGG | + | Chr4:68154430-68154449 | MsG0480022096.01.T01:CDS | 45.0% | |
| TGGAACCATTAAGGAGAAGC+TGG | + | Chr4:68154468-68154487 | MsG0480022096.01.T01:CDS | 45.0% | |
| ! | GGGATGTTGACATCGATTAG+TGG | + | Chr4:68154551-68154570 | MsG0480022096.01.T01:CDS | 45.0% |
| ! | GTTGGGGTTTGGAACCATTA+AGG | + | Chr4:68154459-68154478 | MsG0480022096.01.T01:CDS | 45.0% |
| ! | TGTAGTGGTGGAATCACGTT+GGG | + | Chr4:68154442-68154461 | MsG0480022096.01.T01:CDS | 45.0% |
| ! | TTGTAGTGGTGGAATCACGT+TGG | + | Chr4:68154441-68154460 | MsG0480022096.01.T01:CDS | 45.0% |
| !! | AGTGAAAGCACCGCAAGTAT+AGG | - | Chr4:68154608-68154627 | None:intergenic | 45.0% |
| ! | GTAGTGGTGGAATCACGTTG+GGG | + | Chr4:68154443-68154462 | MsG0480022096.01.T01:CDS | 50.0% |
| GGTGGAATCACGTTGGGGTT+TGG | + | Chr4:68154448-68154467 | MsG0480022096.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 68154371 | 68154745 | 68154371 | ID=MsG0480022096.01;Name=MsG0480022096.01 |
| Chr4 | mRNA | 68154371 | 68154745 | 68154371 | ID=MsG0480022096.01.T01;Parent=MsG0480022096.01;Name=MsG0480022096.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|124 |
| Chr4 | exon | 68154371 | 68154745 | 68154371 | ID=MsG0480022096.01.T01:exon:8441;Parent=MsG0480022096.01.T01 |
| Chr4 | CDS | 68154371 | 68154745 | 68154371 | ID=MsG0480022096.01.T01:cds;Parent=MsG0480022096.01.T01 |
| Gene Sequence |
| Protein sequence |
