AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022210.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022210.01.T01 MTR_4g083360 53.922 102 42 2 8 109 69 165 1.10e-22 92.0
MsG0480022210.01.T01 MTR_4g083360 53.922 102 42 2 8 109 69 165 1.15e-22 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022210.01.T01 AT5G56360 60.759 79 26 2 9 87 77 150 2.38e-19 82.4
MsG0480022210.01.T01 AT2G42390 76.316 38 9 0 9 46 64 101 7.82e-15 67.8

Find 83 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCTTTGATTTCTCAGATT+TGG 0.161023 4:+69732154 MsG0480022210.01.T01:three_prime_UTR
CCAGCCTCCCAGCATGTATT+TGG 0.200115 4:-69729900 None:intergenic
GCCTTTGATTTCTCAGATTT+GGG 0.226353 4:+69732155 MsG0480022210.01.T01:three_prime_UTR
GATTCCGAGGAATGGGTCTT+TGG 0.265562 4:-69728574 None:intergenic
ATTCACTTAATCGACAAATT+AGG 0.272800 4:+69731862 MsG0480022210.01.T01:three_prime_UTR
ATAATCAAGTGCAATTGTTT+TGG 0.281379 4:-69732044 None:intergenic
GAGGAATGGGTCTTTGGGTT+TGG 0.310876 4:-69728568 None:intergenic
CAGCCTCCCAGCATGTATTT+GGG 0.327960 4:-69729899 None:intergenic
AAATATTCTGCAATCACTTA+TGG 0.332341 4:-69732112 None:intergenic
GAGACTGATAACGTAATTAT+TGG 0.351022 4:-69732087 None:intergenic
CTTTGACGCCCTCTTATTTG+TGG 0.382951 4:-69729962 None:intergenic
GTTAGGTAAATTGCAATCAA+TGG 0.390812 4:+69732356 MsG0480022210.01.T01:three_prime_UTR
ATTCCGAGGAATGGGTCTTT+GGG 0.400938 4:-69728573 None:intergenic
ACACAAATATTTAAACAGTC+AGG 0.408591 4:-69732407 None:intergenic
CCAGGTTCATCGGTGCCATC+GGG 0.414346 4:-69728786 None:intergenic
ACCAGGTTCATCGGTGCCAT+CGG 0.429332 4:-69728787 None:intergenic
TTTAGAAATTGAACAAAAGA+GGG 0.443580 4:-69728453 None:intergenic
CAGATTTAAACAAAATGAAA+AGG 0.444212 4:+69732206 MsG0480022210.01.T01:three_prime_UTR
AAAAGATTGCCACAAATAAG+AGG 0.453484 4:+69729953 MsG0480022210.01.T01:CDS
ACTACGCAGATACGTATTGA+TGG 0.473016 4:-69731761 None:intergenic
CCAAATACATGCTGGGAGGC+TGG 0.484058 4:+69729900 MsG0480022210.01.T01:CDS
GTTTAGAAATTGAACAAAAG+AGG 0.488040 4:-69728454 None:intergenic
TGGGAGCGATTCCGAGGAAT+GGG 0.488891 4:-69728581 None:intergenic
TTTCTCAGATTTGGGATGAA+AGG 0.489427 4:+69732163 MsG0480022210.01.T01:three_prime_UTR
CCCGATGGCACCGATGAACC+TGG 0.493453 4:+69728786 MsG0480022210.01.T01:CDS
TTGGGAGCGATTCCGAGGAA+TGG 0.495322 4:-69728582 None:intergenic
AGCGACAGAAAGTCCCGTTA+TGG 0.501578 4:-69728420 None:intergenic
CAAAGTGCCCAAATACATGC+TGG 0.502257 4:+69729892 MsG0480022210.01.T01:CDS
TACATGCTGGGAGGCTGGAA+AGG 0.516392 4:+69729905 MsG0480022210.01.T01:CDS
GGTTTGGATGAAGAAGAGAA+TGG 0.518565 4:-69728552 None:intergenic
TCGATACGCTAGTGCCATAA+CGG 0.520413 4:+69728406 MsG0480022210.01.T01:five_prime_UTR
GTAAATTCTAGTCAAGCTAA+AGG 0.520514 4:-69731791 None:intergenic
TGTAATGTATATAACAGATA+AGG 0.524494 4:-69732552 None:intergenic
GTTTGGATGAAGAAGAGAAT+GGG 0.530013 4:-69728551 None:intergenic
GATGTAATAAGATGTAAAGA+TGG 0.532369 4:+69728714 MsG0480022210.01.T01:five_prime_UTR
TTAAACAAAATGAAAAGGAA+TGG 0.534655 4:+69732211 MsG0480022210.01.T01:three_prime_UTR
ATTAGGTCATAATGAGGTAC+CGG 0.540925 4:+69731879 MsG0480022210.01.T01:three_prime_UTR
TTTGGTGATGATCTTGAATG+AGG 0.553083 4:+69728336 None:intergenic
CGAGTCAATGATGGAATTTG+CGG 0.553365 4:+69728974 MsG0480022210.01.T01:CDS
GATCTAATTTGTCATGTCAG+AGG 0.554623 4:-69732274 None:intergenic
CGAGACGAAAACAGAGACAC+AGG 0.554823 4:-69728953 None:intergenic
CGATACGCTAGTGCCATAAC+GGG 0.555147 4:+69728407 MsG0480022210.01.T01:five_prime_UTR
CACAAATATTTAAACAGTCA+GGG 0.555604 4:-69732406 None:intergenic
TTCGTTATGAGACCATTCAC+TGG 0.561653 4:-69732009 None:intergenic
TTATGTTTAGATTGCTGTGA+TGG 0.562355 4:+69729846 MsG0480022210.01.T01:intron
GAATGCAAAAGAAGCTGAGA+GGG 0.568138 4:+69731679 MsG0480022210.01.T01:intron
TGAAGTCAGACACCAGTGAA+TGG 0.569221 4:+69731997 MsG0480022210.01.T01:three_prime_UTR
ATTCCTCGGAATCGCTCCCA+AGG 0.571269 4:+69728584 MsG0480022210.01.T01:five_prime_UTR
TGATTGCAGAATATTTCTTG+TGG 0.576501 4:+69732119 MsG0480022210.01.T01:three_prime_UTR
AGAATGGAAACCAGGTTCAT+CGG 0.576515 4:-69728796 None:intergenic
CGACAAATTAGGTCATAATG+AGG 0.579984 4:+69731873 MsG0480022210.01.T01:three_prime_UTR
TTGGGATGAAAGGAAACACT+GGG 0.581764 4:+69732173 MsG0480022210.01.T01:three_prime_UTR
TAGAAATTGAACAAAAGAGG+GGG 0.594926 4:-69728451 None:intergenic
TTAGAAATTGAACAAAAGAG+GGG 0.596229 4:-69728452 None:intergenic
AGGTTCATCGGTGCCATCGG+GGG 0.597966 4:-69728784 None:intergenic
AAACCCAAAGACCCATTCCT+CGG 0.598704 4:+69728570 MsG0480022210.01.T01:five_prime_UTR
ATTTGGTATTGCAGGTACAT+CGG 0.601091 4:+69728886 MsG0480022210.01.T01:intron
GCTGATCTTTGTTGAAATTG+CGG 0.603183 4:-69728739 None:intergenic
GAACCTTGGGAGCGATTCCG+AGG 0.607192 4:-69728587 None:intergenic
AAAGTGCCCAAATACATGCT+GGG 0.607920 4:+69729893 MsG0480022210.01.T01:CDS
TTTGGGATGAAAGGAAACAC+TGG 0.609456 4:+69732172 MsG0480022210.01.T01:three_prime_UTR
TCCCAAATCTGAGAAATCAA+AGG 0.610158 4:-69732156 None:intergenic
AACTTGATTTGACAACTGAA+AGG 0.612175 4:+69731721 MsG0480022210.01.T01:CDS
TATTCTGCAATCACTTATGG+AGG 0.622475 4:-69732109 None:intergenic
AGAGGATTAGAAGCACCACA+AGG 0.624859 4:-69732256 None:intergenic
ACGCGAGGAGAAGAGAAATG+AGG 0.624983 4:-69728521 None:intergenic
AAAGATTGCCACAAATAAGA+GGG 0.627594 4:+69729954 MsG0480022210.01.T01:CDS
GATTAGAAGCACCACAAGGG+AGG 0.631673 4:-69732252 None:intergenic
GAGACGAAAACAGAGACACA+GGG 0.636956 4:-69728952 None:intergenic
TTGAAACAATACCTCCCTTG+TGG 0.639848 4:+69732241 MsG0480022210.01.T01:three_prime_UTR
GATGGAAGTGATGAATATGA+TGG 0.639953 4:+69729864 MsG0480022210.01.T01:CDS
AGGTCATAATGAGGTACCGG+AGG 0.644774 4:+69731882 MsG0480022210.01.T01:three_prime_UTR
GGTACATCGGCGTGTCCGCG+TGG 0.653397 4:+69728899 MsG0480022210.01.T01:intron
TCAAGTTTCAGATTCAACAG+TGG 0.654492 4:-69731705 None:intergenic
GGTCATAATGAGGTACCGGA+GGG 0.674045 4:+69731883 MsG0480022210.01.T01:three_prime_UTR
AGAATGCAAAAGAAGCTGAG+AGG 0.679629 4:+69731678 MsG0480022210.01.T01:intron
CAGGTTCATCGGTGCCATCG+GGG 0.682967 4:-69728785 None:intergenic
GAGGATTAGAAGCACCACAA+GGG 0.683863 4:-69732255 None:intergenic
GTGCCCAAATACATGCTGGG+AGG 0.724802 4:+69729896 MsG0480022210.01.T01:CDS
AGAATGGGACGAGAAACGCG+AGG 0.754935 4:-69728536 None:intergenic
AGACGAAAACAGAGACACAG+GGG 0.769467 4:-69728951 None:intergenic
CGACAGTAAAATGTTCCACG+CGG 0.787877 4:-69728914 None:intergenic
TGGGATGAAAGGAAACACTG+GGG 0.804838 4:+69732174 MsG0480022210.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATTTTGTACTTTTTA+AGG + Chr4:69732478-69732497 MsG0480022210.01.T01:three_prime_UTR 10.0%
!! TATAATAGATTTATTTGCAT+TGG + Chr4:69730276-69730295 MsG0480022210.01.T01:intron 15.0%
!! TTAGAAAAAACACTTTAATA+AGG - Chr4:69731083-69731102 None:intergenic 15.0%
!! TTAGATAAAATGTGTAAAAA+TGG - Chr4:69728625-69728644 None:intergenic 15.0%
!! AAAAATCAAGTAAAATGTTG+AGG - Chr4:69731649-69731668 None:intergenic 20.0%
!! AAACAAAATAAAAGAGAGAA+TGG - Chr4:69728815-69728834 None:intergenic 20.0%
!! AACATTGAAAACTTTCTTAT+AGG + Chr4:69731432-69731451 MsG0480022210.01.T01:intron 20.0%
!! ACATACTTGCTTATTTAAAT+AGG - Chr4:69731231-69731250 None:intergenic 20.0%
!! CAGATTTAAACAAAATGAAA+AGG + Chr4:69732206-69732225 MsG0480022210.01.T01:three_prime_UTR 20.0%
!! TGTAATGTATATAACAGATA+AGG - Chr4:69732555-69732574 None:intergenic 20.0%
!! TTAAACAAAATGAAAAGGAA+TGG + Chr4:69732211-69732230 MsG0480022210.01.T01:three_prime_UTR 20.0%
!! TTCATCAATTGAAAATAGAT+GGG - Chr4:69730489-69730508 None:intergenic 20.0%
!! TTTAGAAATTGAACAAAAGA+GGG - Chr4:69728456-69728475 None:intergenic 20.0%
!!! AAAGAAGAAAGTATACTTAA+AGG + Chr4:69730055-69730074 MsG0480022210.01.T01:intron 20.0%
!!! AAGAAGAAAGTATACTTAAA+GGG + Chr4:69730056-69730075 MsG0480022210.01.T01:intron 20.0%
!!! GTTACTTTTTGACATAATTT+TGG + Chr4:69729336-69729355 MsG0480022210.01.T01:intron 20.0%
!!! TATCCTTTAAGACAAAATTA+AGG - Chr4:69730159-69730178 None:intergenic 20.0%
!!! TATTTATCGAGTATTTTTCT+TGG + Chr4:69732064-69732083 MsG0480022210.01.T01:three_prime_UTR 20.0%
!!! TTCAAAAATTATTGTTGTTG+AGG + Chr4:69731583-69731602 MsG0480022210.01.T01:intron 20.0%
!!! TTTTTTGCAAACACAAATTA+AGG + Chr4:69729395-69729414 MsG0480022210.01.T01:intron 20.0%
! AAATATTCTGCAATCACTTA+TGG - Chr4:69732115-69732134 None:intergenic 25.0%
! ACACAAATATTTAAACAGTC+AGG - Chr4:69732410-69732429 None:intergenic 25.0%
! ATGTATAAGGAAATCTGATT+AGG + Chr4:69730310-69730329 MsG0480022210.01.T01:intron 25.0%
! ATTCACTTAATCGACAAATT+AGG + Chr4:69731862-69731881 MsG0480022210.01.T01:three_prime_UTR 25.0%
! ATTGTTTATTCATGTGGATT+TGG + Chr4:69728869-69728888 MsG0480022210.01.T01:intron 25.0%
! ATTGTTTCGTGTTGAATTAT+TGG + Chr4:69728844-69728863 MsG0480022210.01.T01:intron 25.0%
! CACAAATATTTAAACAGTCA+GGG - Chr4:69732409-69732428 None:intergenic 25.0%
! CATATGTGTAATTAAACAGT+TGG - Chr4:69730453-69730472 None:intergenic 25.0%
! CGATGTAGATAGAAAATTAA+CGG + Chr4:69731277-69731296 MsG0480022210.01.T01:intron 25.0%
! CTTGCTTATTTAAATAGGTA+CGG - Chr4:69731226-69731245 None:intergenic 25.0%
! GAGAAAATTGAAGTTAAAGA+GGG + Chr4:69730987-69731006 MsG0480022210.01.T01:intron 25.0%
! GATGTAATAAGATGTAAAGA+TGG + Chr4:69728714-69728733 MsG0480022210.01.T01:five_prime_UTR 25.0%
! GCAACATGACTAAAAATATA+TGG - Chr4:69729598-69729617 None:intergenic 25.0%
! GTTAAAGAAAGAATTCCATT+TGG - Chr4:69730752-69730771 None:intergenic 25.0%
! GTTCATCAATTGAAAATAGA+TGG - Chr4:69730490-69730509 None:intergenic 25.0%
! GTTTAGAAATTGAACAAAAG+AGG - Chr4:69728457-69728476 None:intergenic 25.0%
! TCATAAAGAACAAATAGACA+AGG + Chr4:69730797-69730816 MsG0480022210.01.T01:intron 25.0%
! TGAGAAAATTGAAGTTAAAG+AGG + Chr4:69730986-69731005 MsG0480022210.01.T01:intron 25.0%
! TTAGAAATTGAACAAAAGAG+GGG - Chr4:69728455-69728474 None:intergenic 25.0%
! TTATATCTAATGAACACACA+TGG - Chr4:69731621-69731640 None:intergenic 25.0%
! TTGCAAAAAATAAAGTACAC+CGG - Chr4:69729385-69729404 None:intergenic 25.0%
!! AAAAATTATTGTTGTTGAGG+TGG + Chr4:69731586-69731605 MsG0480022210.01.T01:intron 25.0%
!! AACACAATACTTTTAGATCA+TGG - Chr4:69729713-69729732 None:intergenic 25.0%
!! AGAAGAAAGTATACTTAAAG+GGG + Chr4:69730057-69730076 MsG0480022210.01.T01:intron 25.0%
!! ATAATCAAGTGCAATTGTTT+TGG - Chr4:69732047-69732066 None:intergenic 25.0%
!! ATAGATTTATTTGCATTGGT+AGG + Chr4:69730280-69730299 MsG0480022210.01.T01:intron 25.0%
!! CTACCTTAATTTTGTCTTAA+AGG + Chr4:69730153-69730172 MsG0480022210.01.T01:intron 25.0%
!! GAACACTCAATATGTTTTAT+TGG + Chr4:69729314-69729333 MsG0480022210.01.T01:intron 25.0%
!! GAAGAGGTGAATAAAATTTT+TGG + Chr4:69731542-69731561 MsG0480022210.01.T01:intron 25.0%
!!! AACACTTTCCTTGTTTTAAA+AGG + Chr4:69730595-69730614 MsG0480022210.01.T01:intron 25.0%
!!! CATTTTACTTTGTTTTTCTC+TGG + Chr4:69729445-69729464 MsG0480022210.01.T01:intron 25.0%
!!! TCTAGTTTTTATGATATGAC+TGG - Chr4:69731977-69731996 None:intergenic 25.0%
!!! TGCTGTCAATTTTTTTTCTA+TGG - Chr4:69730216-69730235 None:intergenic 25.0%
!!! TTTTGAAAGCATTAACAATC+TGG + Chr4:69731104-69731123 MsG0480022210.01.T01:intron 25.0%
AAAACCAATGAAGAAGATAC+AGG + Chr4:69730903-69730922 MsG0480022210.01.T01:intron 30.0%
AAAAGATTGCCACAAATAAG+AGG + Chr4:69729953-69729972 MsG0480022210.01.T01:CDS 30.0%
AAAGATTGCCACAAATAAGA+GGG + Chr4:69729954-69729973 MsG0480022210.01.T01:CDS 30.0%
AAATTACAGATTTCACGCTA+TGG + Chr4:69730644-69730663 MsG0480022210.01.T01:intron 30.0%
AAGAAGAGAAAAACAGAAGA+AGG + Chr4:69730849-69730868 MsG0480022210.01.T01:intron 30.0%
AAGTACATCATTACGAGAAA+AGG - Chr4:69730120-69730139 None:intergenic 30.0%
AATCTCTATGCCATATATCT+AGG - Chr4:69729500-69729519 None:intergenic 30.0%
AATTCCTGTATCTTCTTCAT+TGG - Chr4:69730910-69730929 None:intergenic 30.0%
AATTGAAAATAGATGGGTGT+GGG - Chr4:69730483-69730502 None:intergenic 30.0%
AGAAAAAGACCATATTGGAT+AGG + Chr4:69729239-69729258 MsG0480022210.01.T01:intron 30.0%
AGCAAGAACCTTAAAATGTT+CGG + Chr4:69730398-69730417 MsG0480022210.01.T01:intron 30.0%
AGTGTAGATACCTAGATATA+TGG + Chr4:69729487-69729506 MsG0480022210.01.T01:intron 30.0%
ATAATGGTATCGTGGAAATT+GGG + Chr4:69729069-69729088 MsG0480022210.01.T01:intron 30.0%
ATACAAGCTAAGAAACAGAT+TGG - Chr4:69731353-69731372 None:intergenic 30.0%
GAAAAGGCAGAACAAAAAAA+TGG - Chr4:69730104-69730123 None:intergenic 30.0%
GAAGAAGAAAAAGACCATAT+TGG + Chr4:69729234-69729253 MsG0480022210.01.T01:intron 30.0%
GTAAATTCTAGTCAAGCTAA+AGG - Chr4:69731794-69731813 None:intergenic 30.0%
GTATCTATACCTATCCAATA+TGG - Chr4:69729251-69729270 None:intergenic 30.0%
GTTAGGTAAATTGCAATCAA+TGG + Chr4:69732356-69732375 MsG0480022210.01.T01:three_prime_UTR 30.0%
TACAGGAATTGAAAATGAAG+CGG + Chr4:69730920-69730939 MsG0480022210.01.T01:intron 30.0%
TAGAAATTGAACAAAAGAGG+GGG - Chr4:69728454-69728473 None:intergenic 30.0%
TCACAGCAATCTAAACATAA+CGG - Chr4:69729846-69729865 None:intergenic 30.0%
TGATTGCAGAATATTTCTTG+TGG + Chr4:69732119-69732138 MsG0480022210.01.T01:three_prime_UTR 30.0%
TGTTATAGAAATGACGATGA+CGG + Chr4:69728650-69728669 MsG0480022210.01.T01:intron 30.0%
TGTTTAATTACACATATGCC+CGG + Chr4:69730455-69730474 MsG0480022210.01.T01:intron 30.0%
TTATGTTTAGATTGCTGTGA+TGG + Chr4:69729846-69729865 MsG0480022210.01.T01:intron 30.0%
TTCTCGTAATGATGTACTTA+AGG + Chr4:69730121-69730140 MsG0480022210.01.T01:intron 30.0%
TTGTTCTTTATGATCTGCAA+TGG - Chr4:69730790-69730809 None:intergenic 30.0%
! AACTTGATTTGACAACTGAA+AGG + Chr4:69731721-69731740 MsG0480022210.01.T01:CDS 30.0%
! ATTACCTGTCTGTTTTACAA+TGG + Chr4:69730534-69730553 MsG0480022210.01.T01:intron 30.0%
! CAATTTTCTATGCTACCAAA+TGG + Chr4:69730734-69730753 MsG0480022210.01.T01:intron 30.0%
! CTTTGCTGAACTATTTTGTT+AGG + Chr4:69732339-69732358 MsG0480022210.01.T01:three_prime_UTR 30.0%
! GAGACTGATAACGTAATTAT+TGG - Chr4:69732090-69732109 None:intergenic 30.0%
! GATACAGCAATTTGTTTGTT+TGG + Chr4:69730341-69730360 MsG0480022210.01.T01:intron 30.0%
! TTACCTGTCTGTTTTACAAT+GGG + Chr4:69730535-69730554 MsG0480022210.01.T01:intron 30.0%
! TTCTTTTGCATTCTACATTC+AGG - Chr4:69731672-69731691 None:intergenic 30.0%
! TTGGTGATTGTTTATTCATG+TGG + Chr4:69728863-69728882 MsG0480022210.01.T01:intron 30.0%
!! AAAGATGTGAACTTTTGGTT+TGG + Chr4:69730670-69730689 MsG0480022210.01.T01:intron 30.0%
!! CATAATTTTGGTGTAGAAAG+TGG + Chr4:69729348-69729367 MsG0480022210.01.T01:intron 30.0%
!! TGTCATAAACTGACTTTTCT+GGG - Chr4:69731943-69731962 None:intergenic 30.0%
!! TTGTCATAAACTGACTTTTC+TGG - Chr4:69731944-69731963 None:intergenic 30.0%
!!! AAGATGTGAACTTTTGGTTT+GGG + Chr4:69730671-69730690 MsG0480022210.01.T01:intron 30.0%
!!! GCTAAACTGCATTTTTTTGA+AGG + Chr4:69732446-69732465 MsG0480022210.01.T01:three_prime_UTR 30.0%
!!! GGCTTAAACTCTTTTTTACT+GGG + Chr4:69732377-69732396 MsG0480022210.01.T01:three_prime_UTR 30.0%
!!! TGATCTTGCCTTTTAAAACA+AGG - Chr4:69730606-69730625 None:intergenic 30.0%
!!! TGGCTTAAACTCTTTTTTAC+TGG + Chr4:69732376-69732395 MsG0480022210.01.T01:three_prime_UTR 30.0%
AAATAGACAAGGTGAGGAAA+AGG + Chr4:69730808-69730827 MsG0480022210.01.T01:intron 35.0%
AAGAACAAATAGACAAGGTG+AGG + Chr4:69730802-69730821 MsG0480022210.01.T01:intron 35.0%
AAGAGAAAAACAGAAGAAGG+AGG + Chr4:69730852-69730871 MsG0480022210.01.T01:intron 35.0%
AAGGATCCTGTTTGATAAAG+TGG + Chr4:69731520-69731539 MsG0480022210.01.T01:intron 35.0%
ACTTTCTTATAGGATGAAGC+TGG + Chr4:69731442-69731461 MsG0480022210.01.T01:intron 35.0%
AGCAGTTAATTGGGTATCAA+TGG - Chr4:69729753-69729772 None:intergenic 35.0%
ATCCTTCACTTTCAGAATCA+TGG - Chr4:69731506-69731525 None:intergenic 35.0%
ATTAGGTCATAATGAGGTAC+CGG + Chr4:69731879-69731898 MsG0480022210.01.T01:three_prime_UTR 35.0%
ATTTGGTATTGCAGGTACAT+CGG + Chr4:69728886-69728905 MsG0480022210.01.T01:intron 35.0%
CAATTGAAAATAGATGGGTG+TGG - Chr4:69730484-69730503 None:intergenic 35.0%
CGACAAATTAGGTCATAATG+AGG + Chr4:69731873-69731892 MsG0480022210.01.T01:three_prime_UTR 35.0%
CTTTCTTATAGGATGAAGCT+GGG + Chr4:69731443-69731462 MsG0480022210.01.T01:intron 35.0%
GAAATCTGATTAGGAAAGCA+GGG + Chr4:69730319-69730338 MsG0480022210.01.T01:intron 35.0%
GATGGAAGTGATGAATATGA+TGG + Chr4:69729864-69729883 MsG0480022210.01.T01:CDS 35.0%
GCAACACCTTATGTTGTTAT+CGG + Chr4:69729638-69729657 MsG0480022210.01.T01:intron 35.0%
GCGTGAAATCTGTAATTTCA+CGG - Chr4:69730642-69730661 None:intergenic 35.0%
GCTGATCTTTGTTGAAATTG+CGG - Chr4:69728742-69728761 None:intergenic 35.0%
GGTAGGATCAAGTATGTATA+AGG + Chr4:69730297-69730316 MsG0480022210.01.T01:intron 35.0%
GTAAAACAGACAGGTAATAG+TGG - Chr4:69730532-69730551 None:intergenic 35.0%
GTTTGGATGAAGAAGAGAAT+GGG - Chr4:69728554-69728573 None:intergenic 35.0%
TAAAAGAGAGAATGGAAACC+AGG - Chr4:69728807-69728826 None:intergenic 35.0%
TAAGCAGATGAACAGTGTAA+GGG - Chr4:69729665-69729684 None:intergenic 35.0%
TAATGGTATCGTGGAAATTG+GGG + Chr4:69729070-69729089 MsG0480022210.01.T01:intron 35.0%
TACAGCTGCTAAAACAATCT+CGG - Chr4:69729567-69729586 None:intergenic 35.0%
TAGGAGGATGATAAAAGATC+AGG - Chr4:69731178-69731197 None:intergenic 35.0%
TATTCTGCAATCACTTATGG+AGG - Chr4:69732112-69732131 None:intergenic 35.0%
TCAAGTTTCAGATTCAACAG+TGG - Chr4:69731708-69731727 None:intergenic 35.0%
TCCCAAATCTGAGAAATCAA+AGG - Chr4:69732159-69732178 None:intergenic 35.0%
TGTGTTTCATACTCCATCAT+GGG + Chr4:69731385-69731404 MsG0480022210.01.T01:intron 35.0%
TTAAGCAGATGAACAGTGTA+AGG - Chr4:69729666-69729685 None:intergenic 35.0%
TTGTGTTTCATACTCCATCA+TGG + Chr4:69731384-69731403 MsG0480022210.01.T01:intron 35.0%
TTTCTTATAGGATGAAGCTG+GGG + Chr4:69731444-69731463 MsG0480022210.01.T01:intron 35.0%
! ACATTTTACTGTCGTAATGC+TGG + Chr4:69728923-69728942 MsG0480022210.01.T01:CDS 35.0%
! AGACTTTACTGCTTTAGACA+GGG + Chr4:69729034-69729053 MsG0480022210.01.T01:intron 35.0%
! AGCATTAACAATCTGGAATC+TGG + Chr4:69731111-69731130 MsG0480022210.01.T01:intron 35.0%
! AGGGTTTTACATAGCGATAA+TGG + Chr4:69729053-69729072 MsG0480022210.01.T01:intron 35.0%
! AGTCGAGTTGTGCAATTAAA+TGG - Chr4:69731044-69731063 None:intergenic 35.0%
! ATGCTCTTCCGAACATTTTA+AGG - Chr4:69730409-69730428 None:intergenic 35.0%
! CATTTTACTGTCGTAATGCT+GGG + Chr4:69728924-69728943 MsG0480022210.01.T01:CDS 35.0%
! GATAATGGTATCGTGGAAAT+TGG + Chr4:69729068-69729087 MsG0480022210.01.T01:intron 35.0%
! GATCTAATTTGTCATGTCAG+AGG - Chr4:69732277-69732296 None:intergenic 35.0%
! GCCTTTGATTTCTCAGATTT+GGG + Chr4:69732155-69732174 MsG0480022210.01.T01:three_prime_UTR 35.0%
! GGTCTAAAGATGTGAACTTT+TGG + Chr4:69730665-69730684 MsG0480022210.01.T01:intron 35.0%
! TAGACTTTACTGCTTTAGAC+AGG + Chr4:69729033-69729052 MsG0480022210.01.T01:intron 35.0%
! TGATCTTTTATCATCCTCCT+AGG + Chr4:69731177-69731196 MsG0480022210.01.T01:intron 35.0%
! TGCCTTTGATTTCTCAGATT+TGG + Chr4:69732154-69732173 MsG0480022210.01.T01:three_prime_UTR 35.0%
! TTAAGTTGTCAAAACCGCAA+TGG - Chr4:69729113-69729132 None:intergenic 35.0%
! TTGGTTTGAAGTGCTAGATT+AGG + Chr4:69730360-69730379 MsG0480022210.01.T01:intron 35.0%
! TTTCTCAGATTTGGGATGAA+AGG + Chr4:69732163-69732182 MsG0480022210.01.T01:three_prime_UTR 35.0%
!! GTCATAAACTGACTTTTCTG+GGG - Chr4:69731942-69731961 None:intergenic 35.0%
!! TCTGTTTCTTAGCTTGTATG+TGG + Chr4:69731354-69731373 MsG0480022210.01.T01:intron 35.0%
!!! ACTTTTGGTTTGGGTGATAA+AGG + Chr4:69730680-69730699 MsG0480022210.01.T01:intron 35.0%
AAGCAATGAACACGCACATT+TGG + Chr4:69729994-69730013 MsG0480022210.01.T01:intron 40.0%
AAGGGACCGATAACAACATA+AGG - Chr4:69729647-69729666 None:intergenic 40.0%
ACAAAAATAGAAGAGCCCTC+CGG - Chr4:69731901-69731920 None:intergenic 40.0%
ACTACAGGCTAGCAGTTAAT+TGG - Chr4:69729763-69729782 None:intergenic 40.0%
ACTACGCAGATACGTATTGA+TGG - Chr4:69731764-69731783 None:intergenic 40.0%
AGAATGCAAAAGAAGCTGAG+AGG + Chr4:69731678-69731697 MsG0480022210.01.T01:intron 40.0%
AGAATGGAAACCAGGTTCAT+CGG - Chr4:69728799-69728818 None:intergenic 40.0%
AGACATCAAAGACAATTCCG+CGG + Chr4:69730953-69730972 MsG0480022210.01.T01:intron 40.0%
AGCAATCTAAACATAACGGC+AGG - Chr4:69729842-69729861 None:intergenic 40.0%
ATCTAATGAACACACATGGC+AGG - Chr4:69731617-69731636 None:intergenic 40.0%
CATAGTAAACTCCTACTCCT+AGG - Chr4:69731197-69731216 None:intergenic 40.0%
CATATCAACTGCCAGATTGT+GGG + Chr4:69729140-69729159 MsG0480022210.01.T01:intron 40.0%
CCTCTTCCACTTTATCAAAC+AGG - Chr4:69731529-69731548 None:intergenic 40.0%
CCTGTTTGATAAAGTGGAAG+AGG + Chr4:69731526-69731545 MsG0480022210.01.T01:intron 40.0%
CTACAGGCTAGCAGTTAATT+GGG - Chr4:69729762-69729781 None:intergenic 40.0%
GAACCTAAAACTAGGGACAA+GGG + Chr4:69729197-69729216 MsG0480022210.01.T01:intron 40.0%
GAATGCAAAAGAAGCTGAGA+GGG + Chr4:69731679-69731698 MsG0480022210.01.T01:intron 40.0%
GGAAATCTGATTAGGAAAGC+AGG + Chr4:69730318-69730337 MsG0480022210.01.T01:intron 40.0%
GGATAGGTATAGATACGCAT+AGG + Chr4:69729255-69729274 MsG0480022210.01.T01:intron 40.0%
GGCATTCAGCTAATATGAAG+TGG + Chr4:69731132-69731151 MsG0480022210.01.T01:intron 40.0%
GGGGTTGTGAAACAGTTAAA+AGG + Chr4:69730076-69730095 MsG0480022210.01.T01:intron 40.0%
GGTTTGGATGAAGAAGAGAA+TGG - Chr4:69728555-69728574 None:intergenic 40.0%
GTAACAGTGAGATGAACCTT+GGG - Chr4:69728603-69728622 None:intergenic 40.0%
GTGTTTCATACTCCATCATG+GGG + Chr4:69731386-69731405 MsG0480022210.01.T01:intron 40.0%
TAAAGTCCATACCCACAATC+TGG - Chr4:69729154-69729173 None:intergenic 40.0%
TACAATCGAACCTGATCAAC+CGG - Chr4:69731017-69731036 None:intergenic 40.0%
TAGCGACGAACCTAAAACTA+GGG + Chr4:69729190-69729209 MsG0480022210.01.T01:intron 40.0%
TCATATCAACTGCCAGATTG+TGG + Chr4:69729139-69729158 MsG0480022210.01.T01:intron 40.0%
TCTAGAAACTGCACAACTAC+AGG - Chr4:69729778-69729797 None:intergenic 40.0%
TCTCCCATTGTAAAACAGAC+AGG - Chr4:69730541-69730560 None:intergenic 40.0%
TTAGCGACGAACCTAAAACT+AGG + Chr4:69729189-69729208 MsG0480022210.01.T01:intron 40.0%
TTCGTTATGAGACCATTCAC+TGG - Chr4:69732012-69732031 None:intergenic 40.0%
TTGAAACAATACCTCCCTTG+TGG + Chr4:69732241-69732260 MsG0480022210.01.T01:three_prime_UTR 40.0%
TTGGGATGAAAGGAAACACT+GGG + Chr4:69732173-69732192 MsG0480022210.01.T01:three_prime_UTR 40.0%
TTTATCATCCTCCTAGGAGT+AGG + Chr4:69731183-69731202 MsG0480022210.01.T01:intron 40.0%
TTTCTCATGAATACTTGCCG+CGG - Chr4:69730973-69730992 None:intergenic 40.0%
! AAAGTGCCCAAATACATGCT+GGG + Chr4:69729893-69729912 MsG0480022210.01.T01:CDS 40.0%
! AAATGACGATGACGGTTACT+GGG + Chr4:69728658-69728677 MsG0480022210.01.T01:intron 40.0%
! ACATAGCGATAATGGTATCG+TGG + Chr4:69729061-69729080 MsG0480022210.01.T01:intron 40.0%
! AGCCATGATTCTGAAAGTGA+AGG + Chr4:69731501-69731520 MsG0480022210.01.T01:intron 40.0%
! CGAGTCAATGATGGAATTTG+CGG + Chr4:69728974-69728993 MsG0480022210.01.T01:CDS 40.0%
! GAAAGTGGACATTTTGGATC+CGG + Chr4:69729363-69729382 MsG0480022210.01.T01:intron 40.0%
! GGTGTAGAAAGTGGACATTT+TGG + Chr4:69729357-69729376 MsG0480022210.01.T01:intron 40.0%
! TCATGTGGATTTGGTATTGC+AGG + Chr4:69728878-69728897 MsG0480022210.01.T01:intron 40.0%
! TGTCTGTTTTACAATGGGAG+AGG + Chr4:69730540-69730559 MsG0480022210.01.T01:intron 40.0%
! TTTAGGTTCGTCGCTAAAGT+CGG - Chr4:69729186-69729205 None:intergenic 40.0%
! TTTGGGATGAAAGGAAACAC+TGG + Chr4:69732172-69732191 MsG0480022210.01.T01:three_prime_UTR 40.0%
! TTTTCGTCTCGAGTCAATGA+TGG + Chr4:69728965-69728984 MsG0480022210.01.T01:CDS 40.0%
! TTTTGTCGTCGTACAAAGTC+AGG - Chr4:69732526-69732545 None:intergenic 40.0%
!! TTTCCCTTGTCCCTAGTTTT+AGG - Chr4:69729203-69729222 None:intergenic 40.0%
AAAACAGAAGAAGGAGGCTG+AGG + Chr4:69730858-69730877 MsG0480022210.01.T01:intron 45.0%
AAACCCAAAGACCCATTCCT+CGG + Chr4:69728570-69728589 MsG0480022210.01.T01:five_prime_UTR 45.0%
AATGTGTGTGACCTCCATTG+CGG + Chr4:69729096-69729115 MsG0480022210.01.T01:intron 45.0%
AGACGAAAACAGAGACACAG+GGG - Chr4:69728954-69728973 None:intergenic 45.0%
AGTAAACTCCTACTCCTAGG+AGG - Chr4:69731194-69731213 None:intergenic 45.0%
AGTTGTCAAAACCGCAATGG+AGG - Chr4:69729110-69729129 None:intergenic 45.0%
ATTCCGAGGAATGGGTCTTT+GGG - Chr4:69728576-69728595 None:intergenic 45.0%
CGAACCTAAAACTAGGGACA+AGG + Chr4:69729196-69729215 MsG0480022210.01.T01:intron 45.0%
CGACAGTAAAATGTTCCACG+CGG - Chr4:69728917-69728936 None:intergenic 45.0%
GAGACGAAAACAGAGACACA+GGG - Chr4:69728955-69728974 None:intergenic 45.0%
GCTAGATTAGGCGTCTTCTT+TGG + Chr4:69730372-69730391 MsG0480022210.01.T01:intron 45.0%
GGTAACAGTGAGATGAACCT+TGG - Chr4:69728604-69728623 None:intergenic 45.0%
TCGATACGCTAGTGCCATAA+CGG + Chr4:69728406-69728425 MsG0480022210.01.T01:five_prime_UTR 45.0%
TGAAGTCAGACACCAGTGAA+TGG + Chr4:69731997-69732016 MsG0480022210.01.T01:three_prime_UTR 45.0%
TGAAGTTAAAGAGGGCTCTC+CGG + Chr4:69730995-69731014 MsG0480022210.01.T01:intron 45.0%
TGGGATGAAAGGAAACACTG+GGG + Chr4:69732174-69732193 MsG0480022210.01.T01:three_prime_UTR 45.0%
TTAAACCACTGACCCCATGA+TGG - Chr4:69731401-69731420 None:intergenic 45.0%
TTAGGTTCGTCGCTAAAGTC+GGG - Chr4:69729185-69729204 None:intergenic 45.0%
! CAAAGTGCCCAAATACATGC+TGG + Chr4:69729892-69729911 MsG0480022210.01.T01:CDS 45.0%
! CTTTGACGCCCTCTTATTTG+TGG - Chr4:69729965-69729984 None:intergenic 45.0%
! GAAATGACGATGACGGTTAC+TGG + Chr4:69728657-69728676 MsG0480022210.01.T01:intron 45.0%
! GTTTTACACCCATCCATCTG+TGG + Chr4:69731301-69731320 MsG0480022210.01.T01:intron 45.0%
!! AGAGGATTAGAAGCACCACA+AGG - Chr4:69732259-69732278 None:intergenic 45.0%
!! GAGGATTAGAAGCACCACAA+GGG - Chr4:69732258-69732277 None:intergenic 45.0%
!! GGAAAAGGAGCGTTTACAGA+AGG + Chr4:69730823-69730842 MsG0480022210.01.T01:intron 45.0%
AAATAGATGGGTGTGGGCAC+CGG - Chr4:69730477-69730496 None:intergenic 50.0%
ACGCGAGGAGAAGAGAAATG+AGG - Chr4:69728524-69728543 None:intergenic 50.0%
AGAAGAAGGAGGCTGAGGAA+AGG + Chr4:69730863-69730882 MsG0480022210.01.T01:intron 50.0%
AGCGACAGAAAGTCCCGTTA+TGG - Chr4:69728423-69728442 None:intergenic 50.0%
ATACTCCATCATGGGGTCAG+TGG + Chr4:69731393-69731412 MsG0480022210.01.T01:intron 50.0%
CAACTGCCAGATTGTGGGTA+TGG + Chr4:69729145-69729164 MsG0480022210.01.T01:intron 50.0%
CAGCCTCCCAGCATGTATTT+GGG - Chr4:69729902-69729921 None:intergenic 50.0%
CGAGACGAAAACAGAGACAC+AGG - Chr4:69728956-69728975 None:intergenic 50.0%
CGATACGCTAGTGCCATAAC+GGG + Chr4:69728407-69728426 MsG0480022210.01.T01:five_prime_UTR 50.0%
GATTCCGAGGAATGGGTCTT+TGG - Chr4:69728577-69728596 None:intergenic 50.0%
GCAGTGCAACAAGACGAGTA+TGG + Chr4:69729616-69729635 MsG0480022210.01.T01:intron 50.0%
TGGCACTACTTTGTCACGTC+TGG - Chr4:69730512-69730531 None:intergenic 50.0%
! GAGGAATGGGTCTTTGGGTT+TGG - Chr4:69728571-69728590 None:intergenic 50.0%
! TGAACACGCACATTTGGCTC+TGG + Chr4:69730000-69730019 MsG0480022210.01.T01:intron 50.0%
!! AGGTCATAATGAGGTACCGG+AGG + Chr4:69731882-69731901 MsG0480022210.01.T01:three_prime_UTR 50.0%
!! GATTAGAAGCACCACAAGGG+AGG - Chr4:69732255-69732274 None:intergenic 50.0%
!! GGTCATAATGAGGTACCGGA+GGG + Chr4:69731883-69731902 MsG0480022210.01.T01:three_prime_UTR 50.0%
!! TTTGGCTCTGGAGAAGGATG+AGG + Chr4:69730012-69730031 MsG0480022210.01.T01:intron 50.0%
AATAGATGGGTGTGGGCACC+GGG - Chr4:69730476-69730495 None:intergenic 55.0%
ACCCATCCATCTGTGGACAC+TGG + Chr4:69731308-69731327 MsG0480022210.01.T01:intron 55.0%
AGAATGGGACGAGAAACGCG+AGG - Chr4:69728539-69728558 None:intergenic 55.0%
ATTCCTCGGAATCGCTCCCA+AGG + Chr4:69728584-69728603 MsG0480022210.01.T01:five_prime_UTR 55.0%
ATTTCTGCGATTGCCCCCGA+TGG + Chr4:69728771-69728790 MsG0480022210.01.T01:CDS 55.0%
CCAAATACATGCTGGGAGGC+TGG + Chr4:69729900-69729919 MsG0480022210.01.T01:CDS 55.0%
CCAGCCTCCCAGCATGTATT+TGG - Chr4:69729903-69729922 None:intergenic 55.0%
CCATCCATCTGTGGACACTG+GGG + Chr4:69731310-69731329 MsG0480022210.01.T01:intron 55.0%
CCCAGTGTCCACAGATGGAT+GGG - Chr4:69731312-69731331 None:intergenic 55.0%
CCCATCCATCTGTGGACACT+GGG + Chr4:69731309-69731328 MsG0480022210.01.T01:intron 55.0%
GTGCCCAAATACATGCTGGG+AGG + Chr4:69729896-69729915 MsG0480022210.01.T01:CDS 55.0%
TACATGCTGGGAGGCTGGAA+AGG + Chr4:69729905-69729924 MsG0480022210.01.T01:CDS 55.0%
TATGCCCCAGTGTCCACAGA+TGG - Chr4:69731317-69731336 None:intergenic 55.0%
TGGGAGCGATTCCGAGGAAT+GGG - Chr4:69728584-69728603 None:intergenic 55.0%
TTGGGAGCGATTCCGAGGAA+TGG - Chr4:69728585-69728604 None:intergenic 55.0%
!! ACCAGGTTCATCGGTGCCAT+CGG - Chr4:69728790-69728809 None:intergenic 55.0%
!! CGCACATTTGGCTCTGGAGA+AGG + Chr4:69730006-69730025 MsG0480022210.01.T01:intron 55.0%
AGAGGGCTCTCCGGTTGATC+AGG + Chr4:69731004-69731023 MsG0480022210.01.T01:intron 60.0%
CCCCAGTGTCCACAGATGGA+TGG - Chr4:69731313-69731332 None:intergenic 60.0%
GAACCTTGGGAGCGATTCCG+AGG - Chr4:69728590-69728609 None:intergenic 60.0%
!! AGGTTCATCGGTGCCATCGG+GGG - Chr4:69728787-69728806 None:intergenic 60.0%
!! CAGGTTCATCGGTGCCATCG+GGG - Chr4:69728788-69728807 None:intergenic 60.0%
!! CCAGGTTCATCGGTGCCATC+GGG - Chr4:69728789-69728808 None:intergenic 60.0%
!! CCCGATGGCACCGATGAACC+TGG + Chr4:69728786-69728805 MsG0480022210.01.T01:CDS 65.0%
GGTACATCGGCGTGTCCGCG+TGG + Chr4:69728899-69728918 MsG0480022210.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 69728341 69732609 69728341 ID=MsG0480022210.01;Name=MsG0480022210.01
Chr4 mRNA 69728341 69732609 69728341 ID=MsG0480022210.01.T01;Parent=MsG0480022210.01;Name=MsG0480022210.01.T01;_AED=0.49;_eAED=0.49;_QI=338|0.5|0.6|1|0.25|0|5|854|117
Chr4 exon 69728341 69728605 69728341 ID=MsG0480022210.01.T01:exon:59555;Parent=MsG0480022210.01.T01
Chr4 exon 69728692 69728807 69728692 ID=MsG0480022210.01.T01:exon:59556;Parent=MsG0480022210.01.T01
Chr4 exon 69728900 69729006 69728900 ID=MsG0480022210.01.T01:exon:59557;Parent=MsG0480022210.01.T01
Chr4 exon 69729856 69729983 69729856 ID=MsG0480022210.01.T01:exon:59558;Parent=MsG0480022210.01.T01
Chr4 exon 69731680 69732609 69731680 ID=MsG0480022210.01.T01:exon:59559;Parent=MsG0480022210.01.T01
Chr4 five_prime_UTR 69728341 69728605 69728341 ID=MsG0480022210.01.T01:five_prime_utr;Parent=MsG0480022210.01.T01
Chr4 five_prime_UTR 69728692 69728764 69728692 ID=MsG0480022210.01.T01:five_prime_utr;Parent=MsG0480022210.01.T01
Chr4 CDS 69728765 69728807 69728765 ID=MsG0480022210.01.T01:cds;Parent=MsG0480022210.01.T01
Chr4 CDS 69728900 69729006 69728900 ID=MsG0480022210.01.T01:cds;Parent=MsG0480022210.01.T01
Chr4 CDS 69729856 69729983 69729856 ID=MsG0480022210.01.T01:cds;Parent=MsG0480022210.01.T01
Chr4 CDS 69731680 69731755 69731680 ID=MsG0480022210.01.T01:cds;Parent=MsG0480022210.01.T01
Chr4 three_prime_UTR 69731756 69732609 69731756 ID=MsG0480022210.01.T01:three_prime_utr;Parent=MsG0480022210.01.T01
Gene Sequence

>MsG0480022210.01.T01

ATGACGATTTCTGCGATTGCCCCCGATGGCACCGATGAACCTGGTACATCGGCGTGTCCGCGTGGAACATTTTACTGTCGTAATGCTGGGCATTCCCCTGTGTCTCTGTTTTCGTCTCGAGTCAATGATGGAATTTGCGGTGAGCTTAATATTGCTGTGATGGAAGTGATGAATATGATGGTAAAGCAAAGTGCCCAAATACATGCTGGGAGGCTGGAAAGGAAAGCTGAGCGAGAGAAGCTGAGCAAAAAGATTGCCACAAATAAGAGGGCGTCAAAAATGCAAAAGAAGCTGAGAGGGAATCCACTGTTGAATCTGAAACTTGATTTGACAACTGAAAGGCAGAGTTTCTGA
Protein sequence

>MsG0480022210.01.T01

MTISAIAPDGTDEPGTSACPRGTFYCRNAGHSPVSLFSSRVNDGICGELNIAVMEVMNMMVKQSAQIHAGRLERKAEREKLSKKIATNKRASKMQKKLRGNPLLNLKLDLTTERQSF*