Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022249.01.T01 | XP_003607778.1 | 96.479 | 142 | 4 | 1 | 1 | 142 | 1 | 141 | 1.48E-87 | 261 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022249.01.T01 | Q9ZQ80 | 42.636 | 129 | 64 | 3 | 19 | 142 | 64 | 187 | 4.32E-13 | 66.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022249.01.T01 | G7JGC6 | 96.479 | 142 | 4 | 1 | 1 | 142 | 1 | 141 | 7.07e-88 | 261 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480022245.01 | MsG0480022249.01 | 0.812541 | 4.024496e-51 | 2.565997e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022249.01.T01 | MTR_4g082830 | 96.479 | 142 | 4 | 1 | 1 | 142 | 1 | 141 | 1.79e-91 | 261 |
| MsG0480022249.01.T01 | MTR_4g082860 | 65.185 | 135 | 34 | 4 | 10 | 142 | 2 | 125 | 1.74e-42 | 137 |
| MsG0480022249.01.T01 | MTR_5g043660 | 38.053 | 113 | 50 | 5 | 1 | 107 | 1 | 99 | 4.30e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022249.01.T01 | AT2G03440 | 42.636 | 129 | 64 | 3 | 19 | 142 | 64 | 187 | 4.41e-14 | 66.2 |
| MsG0480022249.01.T01 | AT1G13930 | 39.604 | 101 | 47 | 3 | 49 | 142 | 62 | 155 | 2.51e-11 | 58.2 |
| MsG0480022249.01.T01 | AT1G13930 | 39.604 | 101 | 47 | 3 | 49 | 142 | 62 | 155 | 2.51e-11 | 58.2 |
| MsG0480022249.01.T01 | AT1G13930 | 39.604 | 101 | 47 | 3 | 49 | 142 | 62 | 155 | 2.51e-11 | 58.2 |
Find 43 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGCAGCACAATCAAGTTT+AGG | 0.292106 | 4:-70091414 | MsG0480022249.01.T01:CDS |
| AGTTGGTCAGTATGCTAAAT+TGG | 0.398975 | 4:-70091316 | MsG0480022249.01.T01:CDS |
| AGGCAGCACAATCAAGTTTA+GGG | 0.405707 | 4:-70091413 | MsG0480022249.01.T01:CDS |
| TGCTCCACCTTCTTCTAAAC+CGG | 0.409063 | 4:-70091205 | MsG0480022249.01.T01:CDS |
| GTGGCCGCATCATGACCTTT+AGG | 0.409931 | 4:+70091231 | None:intergenic |
| GATGGTTTAGCTAAAGTTGC+TGG | 0.422474 | 4:-70091117 | MsG0480022249.01.T01:CDS |
| TTGGATGATCAGAAAGGGTT+AGG | 0.437630 | 4:-70091297 | MsG0480022249.01.T01:CDS |
| GGTGGAGGAGGTGGACATCA+TGG | 0.442324 | 4:-70091147 | MsG0480022249.01.T01:CDS |
| TGCGCTCGCCATGAGCTCGC+TGG | 0.443061 | 4:+70091449 | None:intergenic |
| TCATCACCTTTGTGATGATC+CGG | 0.443483 | 4:+70091186 | None:intergenic |
| TGATGATCCGGTTTAGAAGA+AGG | 0.446935 | 4:+70091198 | None:intergenic |
| GCGAAGGTGGCTGAGGCTGC+AGG | 0.449162 | 4:-70091354 | MsG0480022249.01.T01:CDS |
| GTTAGGACAGTATGTTGATA+AGG | 0.464040 | 4:-70091280 | MsG0480022249.01.T01:CDS |
| AAGTCTGAAGGTGGAGGAGG+TGG | 0.467957 | 4:-70091156 | MsG0480022249.01.T01:CDS |
| TCATAATAAGCCTATGGATA+AGG | 0.491910 | 4:-70091376 | MsG0480022249.01.T01:CDS |
| CTAAATTGGATGATCAGAAA+GGG | 0.509410 | 4:-70091302 | MsG0480022249.01.T01:CDS |
| GCTAAATTGGATGATCAGAA+AGG | 0.518563 | 4:-70091303 | MsG0480022249.01.T01:CDS |
| TGCAGGTCATAATAAGCCTA+TGG | 0.519120 | 4:-70091382 | MsG0480022249.01.T01:CDS |
| GATTTCAACCAGCGAGCTCA+TGG | 0.520793 | 4:-70091457 | MsG0480022249.01.T01:CDS |
| CTGCATGGTTATCATCCTAA+AGG | 0.528247 | 4:-70091246 | MsG0480022249.01.T01:CDS |
| GGATAAGGCGAAGGTGGCTG+AGG | 0.529556 | 4:-70091361 | MsG0480022249.01.T01:CDS |
| GGAGGTGGACATCATGGGCT+AGG | 0.531113 | 4:-70091141 | MsG0480022249.01.T01:CDS |
| GGAGATCTTCTTGATGCAGT+TGG | 0.535329 | 4:-70091333 | MsG0480022249.01.T01:CDS |
| GTGGAGGAGGTGGACATCAT+GGG | 0.539472 | 4:-70091146 | MsG0480022249.01.T01:CDS |
| GCCACCTTCGCCTTATCCAT+AGG | 0.540837 | 4:+70091366 | None:intergenic |
| GATAAGTCTGAAGGTGGAGG+AGG | 0.559044 | 4:-70091159 | MsG0480022249.01.T01:CDS |
| AGTTTAGGGAAAGGATCTGC+AGG | 0.562101 | 4:-70091399 | MsG0480022249.01.T01:CDS |
| GGACATCATGGGCTAGGTGA+TGG | 0.562487 | 4:-70091135 | MsG0480022249.01.T01:CDS |
| GCCTATGGATAAGGCGAAGG+TGG | 0.566741 | 4:-70091367 | MsG0480022249.01.T01:CDS |
| GCGGATAAGTCTGAAGGTGG+AGG | 0.600426 | 4:-70091162 | MsG0480022249.01.T01:CDS |
| GCACAATCAAGTTTAGGGAA+AGG | 0.600593 | 4:-70091408 | MsG0480022249.01.T01:CDS |
| AGAAGAAGGTGGAGCAGTGG+TGG | 0.601687 | 4:+70091212 | None:intergenic |
| GGTTTAGCTAAAGTTGCTGG+AGG | 0.617052 | 4:-70091114 | MsG0480022249.01.T01:CDS |
| AAGGCTGCTGATTATCTGCA+TGG | 0.619240 | 4:-70091261 | MsG0480022249.01.T01:CDS |
| TAAGCCTATGGATAAGGCGA+AGG | 0.625524 | 4:-70091370 | MsG0480022249.01.T01:CDS |
| AAATCTTCTTCTCTTCGCCG+TGG | 0.629343 | 4:+70091475 | None:intergenic |
| TTTAGAAGAAGGTGGAGCAG+TGG | 0.644489 | 4:+70091209 | None:intergenic |
| TCATCACAAAGGTGATGATG+CGG | 0.659363 | 4:-70091181 | MsG0480022249.01.T01:CDS |
| GATGCGGATAAGTCTGAAGG+TGG | 0.683392 | 4:-70091165 | MsG0480022249.01.T01:CDS |
| TCTAAACCGGATCATCACAA+AGG | 0.690243 | 4:-70091192 | MsG0480022249.01.T01:CDS |
| TCATCCTAAAGGTCATGATG+CGG | 0.692039 | 4:-70091235 | MsG0480022249.01.T01:CDS |
| GATGATGCGGATAAGTCTGA+AGG | 0.699420 | 4:-70091168 | MsG0480022249.01.T01:CDS |
| TGATCCGGTTTAGAAGAAGG+TGG | 0.716579 | 4:+70091201 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTAAATTGGATGATCAGAAA+GGG | - | Chr4:70091306-70091325 | MsG0480022249.01.T01:CDS | 30.0% | |
| TCATAATAAGCCTATGGATA+AGG | - | Chr4:70091232-70091251 | MsG0480022249.01.T01:CDS | 30.0% | |
| AGTTGGTCAGTATGCTAAAT+TGG | - | Chr4:70091292-70091311 | MsG0480022249.01.T01:CDS | 35.0% | |
| GCTAAATTGGATGATCAGAA+AGG | - | Chr4:70091305-70091324 | MsG0480022249.01.T01:CDS | 35.0% | |
| GTTAGGACAGTATGTTGATA+AGG | - | Chr4:70091328-70091347 | MsG0480022249.01.T01:CDS | 35.0% | |
| TCAGAATCTCACAAAAACAC+AGG | - | Chr4:70091107-70091126 | MsG0480022249.01.T01:CDS | 35.0% | |
| AGGCAGCACAATCAAGTTTA+GGG | - | Chr4:70091195-70091214 | MsG0480022249.01.T01:CDS | 40.0% | |
| CTGCATGGTTATCATCCTAA+AGG | - | Chr4:70091362-70091381 | MsG0480022249.01.T01:CDS | 40.0% | |
| GATGGTTTAGCTAAAGTTGC+TGG | - | Chr4:70091491-70091510 | MsG0480022249.01.T01:CDS | 40.0% | |
| GCACAATCAAGTTTAGGGAA+AGG | - | Chr4:70091200-70091219 | MsG0480022249.01.T01:CDS | 40.0% | |
| TCATCACCTTTGTGATGATC+CGG | + | Chr4:70091425-70091444 | None:intergenic | 40.0% | |
| TCATCCTAAAGGTCATGATG+CGG | - | Chr4:70091373-70091392 | MsG0480022249.01.T01:CDS | 40.0% | |
| TCTAAACCGGATCATCACAA+AGG | - | Chr4:70091416-70091435 | MsG0480022249.01.T01:CDS | 40.0% | |
| TGATGATCCGGTTTAGAAGA+AGG | + | Chr4:70091413-70091432 | None:intergenic | 40.0% | |
| TGCAGGTCATAATAAGCCTA+TGG | - | Chr4:70091226-70091245 | MsG0480022249.01.T01:CDS | 40.0% | |
| TTGGATGATCAGAAAGGGTT+AGG | - | Chr4:70091311-70091330 | MsG0480022249.01.T01:CDS | 40.0% | |
| ! | TCATCACAAAGGTGATGATG+CGG | - | Chr4:70091427-70091446 | MsG0480022249.01.T01:CDS | 40.0% |
| AAATCTTCTTCTCTTCGCCG+TGG | + | Chr4:70091136-70091155 | None:intergenic | 45.0% | |
| AGTTTAGGGAAAGGATCTGC+AGG | - | Chr4:70091209-70091228 | MsG0480022249.01.T01:CDS | 45.0% | |
| GAGCGCAAAAATTGTAGCAG+AGG | - | Chr4:70091175-70091194 | MsG0480022249.01.T01:CDS | 45.0% | |
| GAGGCAGCACAATCAAGTTT+AGG | - | Chr4:70091194-70091213 | MsG0480022249.01.T01:CDS | 45.0% | |
| GATGATGCGGATAAGTCTGA+AGG | - | Chr4:70091440-70091459 | MsG0480022249.01.T01:CDS | 45.0% | |
| GGAGATCTTCTTGATGCAGT+TGG | - | Chr4:70091275-70091294 | MsG0480022249.01.T01:CDS | 45.0% | |
| GGTTTAGCTAAAGTTGCTGG+AGG | - | Chr4:70091494-70091513 | MsG0480022249.01.T01:CDS | 45.0% | |
| TAAGCCTATGGATAAGGCGA+AGG | - | Chr4:70091238-70091257 | MsG0480022249.01.T01:CDS | 45.0% | |
| TGATCCGGTTTAGAAGAAGG+TGG | + | Chr4:70091410-70091429 | None:intergenic | 45.0% | |
| TGCTCCACCTTCTTCTAAAC+CGG | - | Chr4:70091403-70091422 | MsG0480022249.01.T01:CDS | 45.0% | |
| TTTAGAAGAAGGTGGAGCAG+TGG | + | Chr4:70091402-70091421 | None:intergenic | 45.0% | |
| ! | AAGGCTGCTGATTATCTGCA+TGG | - | Chr4:70091347-70091366 | MsG0480022249.01.T01:CDS | 45.0% |
| CACAAAAACACAGGCAGCCA+CGG | - | Chr4:70091116-70091135 | MsG0480022249.01.T01:CDS | 50.0% | |
| GATAAGTCTGAAGGTGGAGG+AGG | - | Chr4:70091449-70091468 | MsG0480022249.01.T01:CDS | 50.0% | |
| GATGCGGATAAGTCTGAAGG+TGG | - | Chr4:70091443-70091462 | MsG0480022249.01.T01:CDS | 50.0% | |
| GATTTCAACCAGCGAGCTCA+TGG | - | Chr4:70091151-70091170 | MsG0480022249.01.T01:CDS | 50.0% | |
| AAGTCTGAAGGTGGAGGAGG+TGG | - | Chr4:70091452-70091471 | MsG0480022249.01.T01:CDS | 55.0% | |
| AGAAGAAGGTGGAGCAGTGG+TGG | + | Chr4:70091399-70091418 | None:intergenic | 55.0% | |
| GCCACCTTCGCCTTATCCAT+AGG | + | Chr4:70091245-70091264 | None:intergenic | 55.0% | |
| GCCTATGGATAAGGCGAAGG+TGG | - | Chr4:70091241-70091260 | MsG0480022249.01.T01:CDS | 55.0% | |
| GCGGATAAGTCTGAAGGTGG+AGG | - | Chr4:70091446-70091465 | MsG0480022249.01.T01:CDS | 55.0% | |
| GTGGAGGAGGTGGACATCAT+GGG | - | Chr4:70091462-70091481 | MsG0480022249.01.T01:CDS | 55.0% | |
| GTGGCCGCATCATGACCTTT+AGG | + | Chr4:70091380-70091399 | None:intergenic | 55.0% | |
| !! | GGACATCATGGGCTAGGTGA+TGG | - | Chr4:70091473-70091492 | MsG0480022249.01.T01:CDS | 55.0% |
| GGAGGTGGACATCATGGGCT+AGG | - | Chr4:70091467-70091486 | MsG0480022249.01.T01:CDS | 60.0% | |
| GGATAAGGCGAAGGTGGCTG+AGG | - | Chr4:70091247-70091266 | MsG0480022249.01.T01:CDS | 60.0% | |
| GGTGGAGGAGGTGGACATCA+TGG | - | Chr4:70091461-70091480 | MsG0480022249.01.T01:CDS | 60.0% | |
| GCGAAGGTGGCTGAGGCTGC+AGG | - | Chr4:70091254-70091273 | MsG0480022249.01.T01:CDS | 70.0% | |
| TGCGCTCGCCATGAGCTCGC+TGG | + | Chr4:70091162-70091181 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 70091101 | 70091529 | 70091101 | ID=MsG0480022249.01;Name=MsG0480022249.01 |
| Chr4 | mRNA | 70091101 | 70091529 | 70091101 | ID=MsG0480022249.01.T01;Parent=MsG0480022249.01;Name=MsG0480022249.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|142 |
| Chr4 | exon | 70091101 | 70091529 | 70091101 | ID=MsG0480022249.01.T01:exon:7594;Parent=MsG0480022249.01.T01 |
| Chr4 | CDS | 70091101 | 70091529 | 70091101 | ID=MsG0480022249.01.T01:cds;Parent=MsG0480022249.01.T01 |
| Gene Sequence |
| Protein sequence |
