AlfalfaGEDB | MsG0480022245.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022245.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022245.01.T01	RHN62028.1	96.429	84	3	0	4	87	73	156	1.07E-47	159
MsG0480022245.01.T01	RHN62028.1	81.69	71	13	0	1	71	1	71	5.56E-30	114

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022245.01.T01	A0A396IB81	96.429	84	3	0	4	87	73	156	5.09e-48	159

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048016.01	MsG0480022245.01	0.803315	3.696158e-49	1.851484e-46
MsG0080049011.01	MsG0480022245.01	0.827501	1.510728e-54	1.461147e-51
MsG0080049069.01	MsG0480022245.01	0.842589	2.358070e-58	3.592306e-55
MsG0080049147.01	MsG0480022245.01	0.817502	3.189158e-52	2.326685e-49
MsG0180002140.01	MsG0480022245.01	0.813986	1.938691e-51	1.284940e-48
MsG0180004025.01	MsG0480022245.01	0.822914	1.835275e-53	1.557126e-50
MsG0180004684.01	MsG0480022245.01	0.814013	1.912710e-51	1.268703e-48
MsG0180005106.01	MsG0480022245.01	0.800884	1.168903e-48	5.504885e-46
MsG0180005787.01	MsG0480022245.01	0.818227	2.188549e-52	1.628619e-49
MsG0280011073.01	MsG0480022245.01	0.869026	4.213091e-66	1.557192e-62
MsG0280011287.01	MsG0480022245.01	0.852753	3.752673e-61	7.921462e-58
MsG0280011376.01	MsG0480022245.01	0.802337	5.884507e-49	2.875262e-46
MsG0380013467.01	MsG0480022245.01	0.835916	1.269666e-56	1.575026e-53
MsG0380013471.01	MsG0480022245.01	0.836083	1.151722e-56	1.436093e-53
MsG0380013853.01	MsG0480022245.01	0.819165	1.340475e-52	1.023683e-49
MsG0380014179.01	MsG0480022245.01	0.803276	3.765699e-49	1.884478e-46
MsG0380014727.01	MsG0480022245.01	0.802128	6.499371e-49	3.158588e-46
MsG0480018847.01	MsG0480022245.01	0.815203	1.042474e-51	7.140821e-49
MsG0480018911.01	MsG0480022245.01	0.807356	5.260627e-50	2.923843e-47
MsG0480020024.01	MsG0480022245.01	0.806068	9.840524e-50	5.291099e-47
MsG0480021776.01	MsG0480022245.01	-0.834771	2.472025e-56	2.962706e-53
MsG0480022155.01	MsG0480022245.01	0.832390	9.717344e-56	1.084590e-52
MsG0480022239.01	MsG0480022245.01	0.877377	6.597015e-69	3.310181e-65
MsG0480022240.01	MsG0480022245.01	0.817742	2.816045e-52	2.068032e-49
MsG0480022243.01	MsG0480022245.01	0.930543	1.405032e-93	8.396441e-89
MsG0480022245.01	MsG0480022246.01	0.891821	2.797554e-74	2.491588e-70
MsG0480022245.01	MsG0480022248.01	0.878909	1.917713e-69	1.018909e-65
MsG0480022245.01	MsG0480022249.01	0.812541	4.024496e-51	2.565997e-48
MsG0480022245.01	MsG0480023652.01	0.828251	9.975105e-55	9.860656e-52
MsG0480022245.01	MsG0580024084.01	0.807508	4.884672e-50	2.725734e-47
MsG0480022245.01	MsG0580024597.01	0.902343	1.068521e-78	1.506231e-74
MsG0480022245.01	MsG0580024598.01	0.874901	4.701142e-68	2.153355e-64
MsG0480022245.01	MsG0580024600.01	0.828039	1.121892e-54	1.102289e-51
MsG0480022245.01	MsG0580024601.01	0.879325	1.366439e-69	7.377128e-66
MsG0480022245.01	MsG0580027103.01	0.814899	1.218042e-51	8.274761e-49
MsG0480022245.01	MsG0580029912.01	0.803606	3.217133e-49	1.623723e-46
MsG0480022245.01	MsG0580029914.01	0.845355	4.275560e-59	7.106516e-56
MsG0480022245.01	MsG0680030555.01	0.847061	1.467472e-59	2.577030e-56
MsG0480022245.01	MsG0680030636.01	0.810278	1.248311e-50	7.492684e-48
MsG0480022245.01	MsG0680032516.01	0.857741	1.327271e-62	3.309403e-59
MsG0480022245.01	MsG0680035588.01	0.837237	5.852549e-57	7.554706e-54
MsG0480022245.01	MsG0680035618.01	0.829640	4.597288e-55	4.733118e-52
MsG0480022245.01	MsG0780035960.01	0.816198	6.258705e-52	4.405909e-49
MsG0480022245.01	MsG0780036978.01	0.802186	6.322026e-49	3.076939e-46
MsG0480022245.01	MsG0780037082.01	0.811308	7.472684e-51	4.609455e-48
MsG0480022245.01	MsG0780037330.01	0.809123	2.210218e-50	1.286871e-47
MsG0480022245.01	MsG0780039964.01	0.805139	1.541843e-49	8.094912e-47
MsG0480022245.01	MsG0780040375.01	-0.822041	2.928267e-53	2.423223e-50
MsG0480022245.01	MsG0780040794.01	0.882950	6.773684e-71	4.210035e-67
MsG0480022245.01	MsG0780041015.01	-0.801333	9.461553e-49	4.506501e-46
MsG0480022245.01	MsG0780041729.01	-0.822501	2.290025e-53	1.920251e-50
MsG0480022245.01	MsG0780041747.01	0.821027	5.021962e-53	4.039643e-50
MsG0480022245.01	MsG0880042035.01	0.815757	7.850278e-52	5.460275e-49
MsG0480022245.01	MsG0880042794.01	0.849931	2.354837e-60	4.537096e-57
MsG0480022245.01	MsG0880043829.01	0.855666	5.414933e-62	1.259704e-58
MsG0480022245.01	MsG0880044523.01	0.809251	2.075574e-50	1.212597e-47
MsG0480022245.01	MsG0880044524.01	0.851723	7.372453e-61	1.505900e-57
MsG0480022245.01	MsG0880044564.01	0.811340	7.353707e-51	4.539954e-48
MsG0480022245.01	MsG0880046377.01	0.822806	1.945465e-53	1.645457e-50
MsG0480022245.01	MsG0880046536.01	0.809683	1.676176e-50	9.905885e-48
MsG0480022245.01	MsG0880046877.01	-0.810977	8.812577e-51	5.389732e-48
MsG0480022245.01	MsG0880046963.01	-0.801995	6.919290e-49	3.351323e-46
MsG0480022245.01	MsG0880047152.01	0.813455	2.537436e-51	1.657922e-48
MsG0480022245.01	MsG0880047275.01	0.819899	9.115135e-53	7.105237e-50
MsG0380016117.01	MsG0480022245.01	0.813645	2.305169e-51	1.513854e-48
MsG0380016182.01	MsG0480022245.01	0.816592	5.108798e-52	3.635221e-49
MsG0380016353.01	MsG0480022245.01	0.803832	2.887485e-49	1.465850e-46
MsG0380016740.01	MsG0480022245.01	0.851155	1.066797e-60	2.139522e-57
MsG0380017022.01	MsG0480022245.01	0.856127	3.968579e-62	9.374325e-59
MsG0380017023.01	MsG0480022245.01	0.866336	3.071623e-65	1.031584e-61
MsG0380017037.01	MsG0480022245.01	0.821315	4.310701e-53	3.494987e-50
MsG0380017085.01	MsG0480022245.01	0.854882	9.154637e-62	2.074774e-58
MsG0380017338.01	MsG0480022245.01	0.802519	5.398340e-49	2.649734e-46
MsG0380017512.01	MsG0480022245.01	0.804327	2.278059e-49	1.171222e-46
MsG0380017791.01	MsG0480022245.01	0.872417	3.232800e-67	1.351844e-63
MsG0480018402.01	MsG0480022245.01	0.800736	1.253109e-48	5.879054e-46
MsG0280007165.01	MsG0480022245.01	-0.823712	1.194717e-53	1.036864e-50
MsG0280007429.01	MsG0480022245.01	0.822523	2.263172e-53	1.898970e-50
MsG0280008098.01	MsG0480022245.01	0.803380	3.584425e-49	1.798498e-46
MsG0280008928.01	MsG0480022245.01	0.818514	1.884137e-52	1.413332e-49
MsG0280008929.01	MsG0480022245.01	0.834066	3.716378e-56	4.361163e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022245.01.T01	MTR_4g082883	52.632	76	30	2	16	87	26	99	3.68e-13	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 20 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCACTTGGTAGAAACTTATT+TGG	0.193374	4:+70075030	None:intergenic
CACACCTGCTTCTTCGGTTT+TGG	0.199931	4:+70074849	None:intergenic
GTTTCTACCAAGTGAGGTTT+TGG	0.234806	4:-70075022	MsG0480022245.01.T01:CDS
ATCAAGCACACCTGCTTCTT+CGG	0.347454	4:+70074843	None:intergenic
TTTGGCAGGCTCCACAGGTT+TGG	0.367487	4:+70074867	None:intergenic
CAGTTGTAGAGCAAATTGAT+AGG	0.431190	4:-70074909	MsG0480022245.01.T01:CDS
GAAGAAGCAGGTGTGCTTGA+TGG	0.500199	4:-70074841	MsG0480022245.01.T01:CDS
GCACTCCCCAAAACCTCACT+TGG	0.541506	4:+70075015	None:intergenic
CCTGCCAAAACCGAAGAAGC+AGG	0.546448	4:-70074853	MsG0480022245.01.T01:CDS
GAATCAGCACAAACAACGGT+AGG	0.546825	4:-70074979	MsG0480022245.01.T01:CDS
CAAACAACGGTAGGGAAACA+AGG	0.547166	4:-70074970	MsG0480022245.01.T01:CDS
AAATAAGTTTCTACCAAGTG+AGG	0.567992	4:-70075028	MsG0480022245.01.T01:CDS
AGTTGTAGAGCAAATTGATA+GGG	0.586465	4:-70074908	MsG0480022245.01.T01:CDS
TTATGTTCATTCCAAACCTG+TGG	0.590330	4:-70074878	MsG0480022245.01.T01:CDS
AAGGTTGCAACTTCTGCAGA+GGG	0.615194	4:-70074943	MsG0480022245.01.T01:CDS
GAAGGTTGCAACTTCTGCAG+AGG	0.640521	4:-70074944	MsG0480022245.01.T01:CDS
AGAATAACTATGGCAGCAAG+CGG	0.643714	4:-70075057	None:intergenic
AATCAGCACAAACAACGGTA+GGG	0.654805	4:-70074978	MsG0480022245.01.T01:CDS
GGTAGGGAAACAAGGTGAGA+AGG	0.656228	4:-70074962	MsG0480022245.01.T01:CDS
AGCAGAATCAGCACAAACAA+CGG	0.662614	4:-70074983	MsG0480022245.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAATAAGTTTCTACCAAGTG+AGG	-	Chr4:70074827-70074846	MsG0480022245.01.T01:CDS	30.0%
	AGTTGTAGAGCAAATTGATA+GGG	-	Chr4:70074947-70074966	MsG0480022245.01.T01:CDS	30.0%
	TCACTTGGTAGAAACTTATT+TGG	+	Chr4:70074828-70074847	None:intergenic	30.0%
	CAGTTGTAGAGCAAATTGAT+AGG	-	Chr4:70074946-70074965	MsG0480022245.01.T01:CDS	35.0%
	TTATGTTCATTCCAAACCTG+TGG	-	Chr4:70074977-70074996	MsG0480022245.01.T01:CDS	35.0%
!	GATGGTGTTAAAAAGACTGT+TGG	-	Chr4:70075032-70075051	MsG0480022245.01.T01:CDS	35.0%
!	TTTCTACCAAGTGAGGTTTT+GGG	-	Chr4:70074834-70074853	MsG0480022245.01.T01:CDS	35.0%
	AATCAGCACAAACAACGGTA+GGG	-	Chr4:70074877-70074896	MsG0480022245.01.T01:CDS	40.0%
	AGCAGAATCAGCACAAACAA+CGG	-	Chr4:70074872-70074891	MsG0480022245.01.T01:CDS	40.0%
	GGTGTTAAAAAGACTGTTGG+AGG	-	Chr4:70075035-70075054	MsG0480022245.01.T01:CDS	40.0%
!	GTTTCTACCAAGTGAGGTTT+TGG	-	Chr4:70074833-70074852	MsG0480022245.01.T01:CDS	40.0%
!	TTCTACCAAGTGAGGTTTTG+GGG	-	Chr4:70074835-70074854	MsG0480022245.01.T01:CDS	40.0%
	AAGGTTGCAACTTCTGCAGA+GGG	-	Chr4:70074912-70074931	MsG0480022245.01.T01:CDS	45.0%
	ATCAAGCACACCTGCTTCTT+CGG	+	Chr4:70075015-70075034	None:intergenic	45.0%
	CAAACAACGGTAGGGAAACA+AGG	-	Chr4:70074885-70074904	MsG0480022245.01.T01:CDS	45.0%
	GAATCAGCACAAACAACGGT+AGG	-	Chr4:70074876-70074895	MsG0480022245.01.T01:CDS	45.0%
	GAAGAAGCAGGTGTGCTTGA+TGG	-	Chr4:70075014-70075033	MsG0480022245.01.T01:CDS	50.0%
	GAAGGTTGCAACTTCTGCAG+AGG	-	Chr4:70074911-70074930	MsG0480022245.01.T01:CDS	50.0%
	GGTAGGGAAACAAGGTGAGA+AGG	-	Chr4:70074893-70074912	MsG0480022245.01.T01:CDS	50.0%
!	CACACCTGCTTCTTCGGTTT+TGG	+	Chr4:70075009-70075028	None:intergenic	50.0%
!!!	TGAGGTTTTGGGGAGTGCAA+AGG	-	Chr4:70074845-70074864	MsG0480022245.01.T01:CDS	50.0%
	CCTGCCAAAACCGAAGAAGC+AGG	-	Chr4:70075002-70075021	MsG0480022245.01.T01:CDS	55.0%
	GCACTCCCCAAAACCTCACT+TGG	+	Chr4:70074843-70074862	None:intergenic	55.0%
	TTTGGCAGGCTCCACAGGTT+TGG	+	Chr4:70074991-70075010	None:intergenic	55.0%
!!	CCTGCTTCTTCGGTTTTGGC+AGG	+	Chr4:70075005-70075024	None:intergenic	55.0%
!!!	GGTTTTGGGGAGTGCAAAGG+TGG	-	Chr4:70074848-70074867	MsG0480022245.01.T01:CDS	55.0%
!!	TCGGTTTTGGCAGGCTCCAC+AGG	+	Chr4:70074996-70075015	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	70074807	70075070	70074807	ID=MsG0480022245.01;Name=MsG0480022245.01
Chr4	mRNA	70074807	70075070	70074807	ID=MsG0480022245.01.T01;Parent=MsG0480022245.01;Name=MsG0480022245.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|87
Chr4	exon	70074807	70075070	70074807	ID=MsG0480022245.01.T01:exon:11101;Parent=MsG0480022245.01.T01
Chr4	CDS	70074807	70075070	70074807	ID=MsG0480022245.01.T01:cds;Parent=MsG0480022245.01.T01

Gene Sequence	>MsG0480022245.01.T01 ATGGCAGCAAGCGGAGAACCAAATAAGTTTCTACCAAGTGAGGTTTTGGGGAGTGCAAAGGTGGCAGCAGAATCAGCACAAACAACGGTAGGGAAACAAGGTGAGAAGGTTGCAACTTCTGCAGAGGGTGTTAAGAAGACAGTTGTAGAGCAAATTGATAGGGCTGCTGATTATGTTCATTCCAAACCTGTGGAGCCTGCCAAAACCGAAGAAGCAGGTGTGCTTGATGGTGTTAAAAAGACTGTTGGAGGCTTATTCAAATGA
Protein sequence	>MsG0480022245.01.T01 MAASGEPNKFLPSEVLGSAKVAAESAQTTVGKQGEKVATSAEGVKKTVVEQIDRAADYVHSKPVEPAKTEEAGVLDGVKKTVGGLFK*