Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380012471.01 | MsG0480022314.01 | 0.808916 | 2.447974e-50 | 1.417312e-47 |
| MsG0480022314.01 | MsG0780036815.01 | 0.808823 | 2.563254e-50 | 1.480403e-47 |
| MsG0480022314.01 | MsG0780039137.01 | 0.802651 | 5.070500e-49 | 2.497226e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTTGTATGCACTGTTTA+TGG | 0.086360 | 4:+71040676 | MsG0480022314.01.T01:CDS |
| ACATTTAACTTAAGGCAAAT+TGG | 0.319681 | 4:+71040626 | MsG0480022314.01.T01:intron |
| TGCATGTCAGTCCCTCCTTC+TGG | 0.339704 | 4:+71040724 | MsG0480022314.01.T01:CDS |
| GATATTGAGGTGCATCATAT+AGG | 0.493772 | 4:-71040700 | None:intergenic |
| ATGCATGCTAAGACTGATGC+TGG | 0.538290 | 4:+71040486 | MsG0480022314.01.T01:CDS |
| TTGTTCAGTTCATGACCAGA+AGG | 0.569711 | 4:-71040739 | None:intergenic |
| TTCAGTTCATGACCAGAAGG+AGG | 0.578581 | 4:-71040736 | None:intergenic |
| GACTGACATGCATGATATTG+AGG | 0.585893 | 4:-71040713 | None:intergenic |
| TCAGTTCATGACCAGAAGGA+GGG | 0.610974 | 4:-71040735 | None:intergenic |
| CTAAGACTGATGCTGGCTTG+AGG | 0.646313 | 4:+71040493 | MsG0480022314.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTAGTTACAATAAATTTT+TGG | - | Chr4:71040595-71040614 | None:intergenic | 10.0% |
| !! | TTAAGTTAAATGTCTAAATT+CGG | - | Chr4:71040619-71040638 | None:intergenic | 15.0% |
| !!! | TTGACTAATTCTTTTTATTT+GGG | + | Chr4:71040400-71040419 | MsG0480022314.01.T01:intron | 15.0% |
| !! | ATTCTTTCTTGTAATAACTT+AGG | + | Chr4:71040555-71040574 | MsG0480022314.01.T01:intron | 20.0% |
| !!! | GAATTTAGACATTTAACTTA+AGG | + | Chr4:71040618-71040637 | MsG0480022314.01.T01:intron | 20.0% |
| !!! | GTTGACTAATTCTTTTTATT+TGG | + | Chr4:71040399-71040418 | MsG0480022314.01.T01:intron | 20.0% |
| !!! | TGACTAATTCTTTTTATTTG+GGG | + | Chr4:71040401-71040420 | MsG0480022314.01.T01:intron | 20.0% |
| ! | ATAACTTAGGATCAATACTT+AGG | + | Chr4:71040568-71040587 | MsG0480022314.01.T01:intron | 25.0% |
| !! | ACATTTAACTTAAGGCAAAT+TGG | + | Chr4:71040626-71040645 | MsG0480022314.01.T01:intron | 25.0% |
| !!! | ACTAATTCTTTTTATTTGGG+GGG | + | Chr4:71040403-71040422 | MsG0480022314.01.T01:intron | 25.0% |
| !!! | GACTAATTCTTTTTATTTGG+GGG | + | Chr4:71040402-71040421 | MsG0480022314.01.T01:intron | 25.0% |
| AATCTTGTATGCACTGTTTA+TGG | + | Chr4:71040676-71040695 | MsG0480022314.01.T01:CDS | 30.0% | |
| GTAACTTAATATTGTCTGCA+GGG | + | Chr4:71040434-71040453 | MsG0480022314.01.T01:intron | 30.0% | |
| TGTAACTTAATATTGTCTGC+AGG | + | Chr4:71040433-71040452 | MsG0480022314.01.T01:intron | 30.0% | |
| GATATTGAGGTGCATCATAT+AGG | - | Chr4:71040703-71040722 | None:intergenic | 35.0% | |
| GACTGACATGCATGATATTG+AGG | - | Chr4:71040716-71040735 | None:intergenic | 40.0% | |
| ATGCATGCTAAGACTGATGC+TGG | + | Chr4:71040486-71040505 | MsG0480022314.01.T01:CDS | 45.0% | |
| TCAGTTCATGACCAGAAGGA+GGG | - | Chr4:71040738-71040757 | None:intergenic | 45.0% | |
| !! | CTAAGACTGATGCTGGCTTG+AGG | + | Chr4:71040493-71040512 | MsG0480022314.01.T01:CDS | 50.0% |
| TGCATGTCAGTCCCTCCTTC+TGG | + | Chr4:71040724-71040743 | MsG0480022314.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 71040382 | 71040757 | 71040382 | ID=MsG0480022314.01;Name=MsG0480022314.01 |
| Chr4 | mRNA | 71040382 | 71040757 | 71040382 | ID=MsG0480022314.01.T01;Parent=MsG0480022314.01;Name=MsG0480022314.01.T01;_AED=0.48;_eAED=0.52;_QI=0|0|0|1|0|0|3|0|68 |
| Chr4 | exon | 71040382 | 71040395 | 71040382 | ID=MsG0480022314.01.T01:exon:2444;Parent=MsG0480022314.01.T01 |
| Chr4 | exon | 71040455 | 71040529 | 71040455 | ID=MsG0480022314.01.T01:exon:2445;Parent=MsG0480022314.01.T01 |
| Chr4 | exon | 71040640 | 71040757 | 71040640 | ID=MsG0480022314.01.T01:exon:2446;Parent=MsG0480022314.01.T01 |
| Chr4 | CDS | 71040382 | 71040395 | 71040382 | ID=MsG0480022314.01.T01:cds;Parent=MsG0480022314.01.T01 |
| Chr4 | CDS | 71040455 | 71040529 | 71040455 | ID=MsG0480022314.01.T01:cds;Parent=MsG0480022314.01.T01 |
| Chr4 | CDS | 71040640 | 71040757 | 71040640 | ID=MsG0480022314.01.T01:cds;Parent=MsG0480022314.01.T01 |
| Gene Sequence |
| Protein sequence |
