AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022399.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022399.01.T01 MTR_4g088885 96.178 314 12 0 16 329 437 750 0.0 620
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022399.01.T01 AT4G04910 80.317 315 62 0 16 330 427 741 0.0 540

Find 81 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGGCTCAATTGTCTATTTA+AGG 0.168925 4:-72045065 None:intergenic
TGTACATTAATCCAGATTAT+TGG 0.195042 4:+72047115 MsG0480022399.01.T01:intron
TCCCCTTGTTACTTGTCTTC+TGG 0.239207 4:+72045983 MsG0480022399.01.T01:CDS
AACAAGTGAAGTGGACTTCT+TGG 0.264786 4:+72047495 MsG0480022399.01.T01:CDS
CATGAAGTTATTCCTGCATT+TGG 0.290918 4:+72045157 MsG0480022399.01.T01:CDS
TATGACAAACCTTAATAATC+TGG 0.308087 4:-72046747 None:intergenic
GGGACAGGTGTTTGTAGTTC+AGG 0.314763 4:+72048626 MsG0480022399.01.T01:three_prime_UTR
CATACGTTGCATAGTACTTC+TGG 0.332984 4:+72048548 MsG0480022399.01.T01:three_prime_UTR
TAAATGTGCCCAGATTATTA+AGG 0.340164 4:+72046738 MsG0480022399.01.T01:CDS
TCTGCAGAAGCTATCTATTC+TGG 0.352769 4:+72048377 MsG0480022399.01.T01:CDS
AGTGGAGGAGGAAAATATTA+AGG 0.367855 4:+72045105 MsG0480022399.01.T01:CDS
GTTATACATGCTTATTGAAA+TGG 0.368474 4:+72048331 MsG0480022399.01.T01:CDS
AATATATCTATATCACAATA+AGG 0.383832 4:-72048745 None:intergenic
TGTAGTGGTAAAACCGCAAT+TGG 0.390883 4:+72046098 MsG0480022399.01.T01:intron
CACTACTGGTCTTGCTCAAA+CGG 0.404488 4:+72047183 MsG0480022399.01.T01:CDS
TGAGGAGGCTTTGTCAATCA+TGG 0.415645 4:+72048578 MsG0480022399.01.T01:three_prime_UTR
TGAATTGAGCTGTACCAAAC+TGG 0.418111 4:+72048677 MsG0480022399.01.T01:three_prime_UTR
ATCATTTCTACGATTGCCTC+GGG 0.421164 4:+72048420 MsG0480022399.01.T01:CDS
TTCTGGAAGGTTCTCGTGGC+AGG 0.428089 4:+72046000 MsG0480022399.01.T01:CDS
TGATTCTGCCGCAGAGGCAT+TGG 0.438599 4:+72047927 MsG0480022399.01.T01:CDS
AGGTTACGCTTATGCCAGTT+TGG 0.439097 4:-72048691 None:intergenic
CAAATGCAGGAATAACTTCA+TGG 0.439399 4:-72045156 None:intergenic
ATCCAGATTATTGGAGTATG+TGG 0.445258 4:+72047124 MsG0480022399.01.T01:intron
ATTTCAGCTGAAACAATGAT+TGG 0.454411 4:+72046700 MsG0480022399.01.T01:CDS
CTTCGCGTCATGTTTGCTCA+AGG 0.455642 4:-72047566 None:intergenic
CTTGTTACTTGTCTTCTGGA+AGG 0.464285 4:+72045987 MsG0480022399.01.T01:CDS
CGCGTCATGTTTGCTCAAGG+TGG 0.470631 4:-72047563 None:intergenic
CATAATTTCGCAGACACTAC+TGG 0.471918 4:+72047169 MsG0480022399.01.T01:CDS
CTGGTTTAGTGAGGTCATCT+AGG 0.475218 4:-72045084 None:intergenic
GCTCAAACGGCTTCCTCCAA+AGG 0.476198 4:+72047196 MsG0480022399.01.T01:CDS
AGCATGTATAACTTCTTGAT+AGG 0.486862 4:-72048320 None:intergenic
GTGGACTTCTTGGATTCACT+TGG 0.489526 4:+72047505 MsG0480022399.01.T01:CDS
CTGACGATCTTGAGCGATGC+AGG 0.490071 4:+72045188 MsG0480022399.01.T01:CDS
TACAGTGGTGCGGAACTTGA+AGG 0.495507 4:+72045016 MsG0480022399.01.T01:CDS
CAGCTGCACAAGAAAAGAAA+AGG 0.511319 4:-72044979 None:intergenic
TGTGTAAATTAACATTGTAC+AGG 0.513888 4:+72048787 MsG0480022399.01.T01:three_prime_UTR
CTCTGAATTTACAGACTCCA+TGG 0.521657 4:+72045876 MsG0480022399.01.T01:intron
ATGACAAACCTTAATAATCT+GGG 0.522191 4:-72046746 None:intergenic
AATCATTTCTACGATTGCCT+CGG 0.528692 4:+72048419 MsG0480022399.01.T01:CDS
GAGCAAACATGACGCGAAGA+AGG 0.528847 4:+72047570 MsG0480022399.01.T01:CDS
ATGGCAGCGCAAGGTGCGCA+AGG 0.530513 4:+72048350 MsG0480022399.01.T01:CDS
GCGTCATGTTTGCTCAAGGT+GGG 0.542507 4:-72047562 None:intergenic
TAAGCGTAACCTTCTGTAGT+GGG 0.545084 4:+72048702 MsG0480022399.01.T01:three_prime_UTR
CCTCACTAAACCAGTGGAGG+AGG 0.549326 4:+72045093 MsG0480022399.01.T01:CDS
TTGCTGATAGTAATCGGGAC+AGG 0.552329 4:+72048611 MsG0480022399.01.T01:three_prime_UTR
GGAAAATATTAAGGTTACGA+TGG 0.553010 4:+72045114 MsG0480022399.01.T01:CDS
TCAGTGGAAGCTCCAAATGC+AGG 0.554433 4:-72045169 None:intergenic
CCTCCTCCACTGGTTTAGTG+AGG 0.558598 4:-72045093 None:intergenic
CTTATTGAAATGGCAGCGCA+AGG 0.558800 4:+72048341 MsG0480022399.01.T01:CDS
TTATTGGAGTATGTGGCCAT+TGG 0.567433 4:+72047131 MsG0480022399.01.T01:CDS
CACCACACTCCACCATGCCA+TGG 0.567836 4:-72045893 None:intergenic
CTTCCAGAAGACAAGTAACA+AGG 0.583537 4:-72045986 None:intergenic
AAGAAAAGGTAAACCTCCCA+TGG 0.585589 4:-72044965 None:intergenic
TGTTGAATCCCCACTACAGA+AGG 0.586139 4:-72048711 None:intergenic
GCAGCGCAAGGTGCGCAAGG+TGG 0.594295 4:+72048353 MsG0480022399.01.T01:CDS
ATAAGCGTAACCTTCTGTAG+TGG 0.596263 4:+72048701 MsG0480022399.01.T01:three_prime_UTR
GCATCGCTCAAGATCGTCAG+TGG 0.600105 4:-72045185 None:intergenic
TATTGGAACAACAAGTGAAG+TGG 0.601641 4:+72047486 MsG0480022399.01.T01:CDS
TCGAGAACAATGACACTCAA+AGG 0.606331 4:-72047013 None:intergenic
TTGTTCTCGATGACATTGAG+AGG 0.606968 4:+72047026 MsG0480022399.01.T01:CDS
TCAGAGGGCAATGCTAATTG+CGG 0.615431 4:+72045935 MsG0480022399.01.T01:CDS
GAGCAAGTTAAAGTGAGCAA+AGG 0.616725 4:+72045957 MsG0480022399.01.T01:CDS
GGACAAACAGAAACCTTTGG+AGG 0.617329 4:-72047209 None:intergenic
GCCTACAGTAGCTCCAATTG+CGG 0.618003 4:-72046111 None:intergenic
TGTCTTCTGGAAGGTTCTCG+TGG 0.622423 4:+72045996 MsG0480022399.01.T01:CDS
GACATAAGCACATTTATCAG+AGG 0.624277 4:+72045919 MsG0480022399.01.T01:CDS
ACATAAGCACATTTATCAGA+GGG 0.625578 4:+72045920 MsG0480022399.01.T01:CDS
TTCCAGAAGACAAGTAACAA+GGG 0.638523 4:-72045985 None:intergenic
TACATTATCCAATGCCTCTG+CGG 0.645357 4:-72047935 None:intergenic
GATATTCAATTAAAACACAA+TGG 0.645598 4:-72048505 None:intergenic
CTCCATGGCATGGTGGAGTG+TGG 0.652735 4:+72045891 MsG0480022399.01.T01:CDS
TGACCTCACTAAACCAGTGG+AGG 0.654623 4:+72045090 MsG0480022399.01.T01:CDS
TCCAGAAGACAAGTAACAAG+GGG 0.658987 4:-72045984 None:intergenic
AATTTACAGACTCCATGGCA+TGG 0.661477 4:+72045881 MsG0480022399.01.T01:intron
AGATGACCTCACTAAACCAG+TGG 0.669273 4:+72045087 MsG0480022399.01.T01:CDS
AGATATTGATTCTGCCGCAG+AGG 0.669888 4:+72047921 MsG0480022399.01.T01:CDS
ACCGCAATTGGAGCTACTGT+AGG 0.682519 4:+72046110 MsG0480022399.01.T01:CDS
AAGCGTAACCTTCTGTAGTG+GGG 0.684201 4:+72048703 MsG0480022399.01.T01:three_prime_UTR
TTACAGACTCCATGGCATGG+TGG 0.726184 4:+72045884 MsG0480022399.01.T01:intron
TCATTTCTACGATTGCCTCG+GGG 0.730434 4:+72048421 MsG0480022399.01.T01:CDS
CTAATCTGACAACATCCCCG+AGG 0.780848 4:-72048436 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAATGTATAAATAAA+GGG - Chr4:72045590-72045609 None:intergenic 10.0%
!! TAAGAAAAATGTATAAATAA+AGG - Chr4:72045591-72045610 None:intergenic 10.0%
!!! ATTAATATTTCTAATATTGA+AGG - Chr4:72047381-72047400 None:intergenic 10.0%
!! AAAGTAGAATATATTGTATT+GGG - Chr4:72045566-72045585 None:intergenic 15.0%
!! AAATATCAAATAAAAAGGAA+TGG + Chr4:72046584-72046603 MsG0480022399.01.T01:intron 15.0%
!! AATATATCTATATCACAATA+AGG - Chr4:72048748-72048767 None:intergenic 15.0%
!! AATTAGAGTTATATTGTATT+GGG - Chr4:72045525-72045544 None:intergenic 15.0%
!! AGAAAAATGTATAAATAAAG+GGG - Chr4:72045589-72045608 None:intergenic 15.0%
!! AGTTAAAACATCAAATAAAA+AGG - Chr4:72046196-72046215 None:intergenic 15.0%
!! ATTAAAACTTAAAATACTTG+AGG - Chr4:72047291-72047310 None:intergenic 15.0%
!! TAATTAGAGTTATATTGTAT+TGG - Chr4:72045526-72045545 None:intergenic 15.0%
!!! AATACCCTTATTTTATTTTT+TGG - Chr4:72046501-72046520 None:intergenic 15.0%
!!! ACTTTTTCTTGTAAAATTAT+AGG - Chr4:72048042-72048061 None:intergenic 15.0%
!!! TGTTTATCATGTAAATTTTA+AGG + Chr4:72046948-72046967 MsG0480022399.01.T01:intron 15.0%
!!! TTTTTCATTTGTAAAATTAC+AGG + Chr4:72047867-72047886 MsG0480022399.01.T01:intron 15.0%
!!! TTTTTTCATCCAAAAAATAA+TGG - Chr4:72046346-72046365 None:intergenic 15.0%
!! AAACATCAAATAAAAAGGAA+TGG - Chr4:72046191-72046210 None:intergenic 20.0%
!! AATGAAAATAGTTGTAGAAT+TGG - Chr4:72046529-72046548 None:intergenic 20.0%
!! AATGAAATAGTTAATACTTG+GGG - Chr4:72047096-72047115 None:intergenic 20.0%
!! ACAATGAAATAGTTAATACT+TGG - Chr4:72047098-72047117 None:intergenic 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG - Chr4:72045493-72045512 None:intergenic 20.0%
!! ATAAAGTTTGAGCATAAAAT+AGG - Chr4:72048291-72048310 None:intergenic 20.0%
!! CAATGAAATAGTTAATACTT+GGG - Chr4:72047097-72047116 None:intergenic 20.0%
!! GAAAGTAGAATATATTGTAT+TGG - Chr4:72045567-72045586 None:intergenic 20.0%
!! GATATTCAATTAAAACACAA+TGG - Chr4:72048508-72048527 None:intergenic 20.0%
!! GGCTAAAATATCAAATAAAA+AGG + Chr4:72046579-72046598 MsG0480022399.01.T01:intron 20.0%
!! TATTGCTAAGACAAAATAAA+TGG - Chr4:72047989-72048008 None:intergenic 20.0%
!! TTAATTACGTTATCAATTGT+TGG + Chr4:72046310-72046329 MsG0480022399.01.T01:intron 20.0%
!!! ATTTGATGTTTTAGAATTGT+TGG + Chr4:72046236-72046255 MsG0480022399.01.T01:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG - Chr4:72045456-72045475 None:intergenic 20.0%
!!! TAAATTTTCAGAATACCAAT+GGG - Chr4:72045284-72045303 None:intergenic 20.0%
!!! TAGTTTTAACGTTTTATATC+AGG + Chr4:72048812-72048831 MsG0480022399.01.T01:three_prime_UTR 20.0%
!!! TGCTTTTGACTTTATAATTA+AGG + Chr4:72046643-72046662 MsG0480022399.01.T01:intron 20.0%
!!! TTTTATTTATTCTTTCCCAT+TGG + Chr4:72045266-72045285 MsG0480022399.01.T01:intron 20.0%
! AACGTAATTAATTGTGACAA+GGG - Chr4:72046281-72046300 None:intergenic 25.0%
! ACTCAATAGGTTAAAAAGAA+TGG - Chr4:72046783-72046802 None:intergenic 25.0%
! ATATACATGCAATACTCAAT+AGG - Chr4:72046796-72046815 None:intergenic 25.0%
! ATCAAATAAAAAGGAATGGA+GGG + Chr4:72046588-72046607 MsG0480022399.01.T01:intron 25.0%
! ATCAAATAAAAAGGAATGGA+GGG - Chr4:72046607-72046588 None:intergenic 25.0%
! ATGACAAACCTTAATAATCT+GGG - Chr4:72046749-72046768 None:intergenic 25.0%
! ATTATTAGTAGTATAGGTGT+TGG + Chr4:72048094-72048113 MsG0480022399.01.T01:intron 25.0%
! GAAATTATGTTTGAAAAACG+AGG - Chr4:72047158-72047177 None:intergenic 25.0%
! GCAGCCAAAAAATAAAATAA+GGG + Chr4:72046494-72046513 MsG0480022399.01.T01:intron 25.0%
! GTGAAGATTATTAGTAGTAT+AGG + Chr4:72048088-72048107 MsG0480022399.01.T01:intron 25.0%
! GTTATACATGCTTATTGAAA+TGG + Chr4:72048331-72048350 MsG0480022399.01.T01:CDS 25.0%
! TAAATGGATGAAGCAAATAA+AGG - Chr4:72047973-72047992 None:intergenic 25.0%
! TAACGTAATTAATTGTGACA+AGG - Chr4:72046282-72046301 None:intergenic 25.0%
! TAATTACATGCTATTGCATA+TGG + Chr4:72047634-72047653 MsG0480022399.01.T01:intron 25.0%
! TACTACTAATAATCTTCACA+TGG - Chr4:72048087-72048106 None:intergenic 25.0%
! TATAAGCAAAAGCAACAATT+TGG - Chr4:72047808-72047827 None:intergenic 25.0%
! TATCAAATAAAAAGGAATGG+AGG + Chr4:72046587-72046606 MsG0480022399.01.T01:intron 25.0%
! TATGACAAACCTTAATAATC+TGG - Chr4:72046750-72046769 None:intergenic 25.0%
! TCCAAATCTTCTTAAATGAA+CGG - Chr4:72046441-72046460 None:intergenic 25.0%
! TGTACATTAATCCAGATTAT+TGG + Chr4:72047115-72047134 MsG0480022399.01.T01:intron 25.0%
! TGTGTAAATTAACATTGTAC+AGG + Chr4:72048787-72048806 MsG0480022399.01.T01:three_prime_UTR 25.0%
! TTCATCCAAAAAATAATGGT+GGG - Chr4:72046342-72046361 None:intergenic 25.0%
! TTCCTTAATATGTGTGTAAT+CGG + Chr4:72046558-72046577 MsG0480022399.01.T01:intron 25.0%
! TTTCATCCAAAAAATAATGG+TGG - Chr4:72046343-72046362 None:intergenic 25.0%
!! ATTTTGTCTTAGCAATAGTT+TGG + Chr4:72047992-72048011 MsG0480022399.01.T01:intron 25.0%
!! GTAAATTTTCAGAATACCAA+TGG - Chr4:72045285-72045304 None:intergenic 25.0%
!! TATTTCTAATATTGAAGGTG+TGG - Chr4:72047376-72047395 None:intergenic 25.0%
!! TTTTTATCTTTTTGCCAATG+AGG + Chr4:72048134-72048153 MsG0480022399.01.T01:intron 25.0%
!!! ATTTTGAGAAGAAAAAAGCA+AGG + Chr4:72046461-72046480 MsG0480022399.01.T01:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG - Chr4:72045457-72045476 None:intergenic 25.0%
!!! TGAATGTTTCTTGTTATTGT+TGG + Chr4:72047660-72047679 MsG0480022399.01.T01:intron 25.0%
AAAAAAGCAAGGAGTAATCA+TGG + Chr4:72046472-72046491 MsG0480022399.01.T01:intron 30.0%
AAATCTTCTTAAATGAACGG+TGG - Chr4:72046438-72046457 None:intergenic 30.0%
AAGGTAAACTCATTTGTTCT+CGG + Chr4:72047589-72047608 MsG0480022399.01.T01:intron 30.0%
AATCTTCTTAAATGAACGGT+GGG - Chr4:72046437-72046456 None:intergenic 30.0%
AATTGTGACAAGGGTAAAAT+TGG - Chr4:72046272-72046291 None:intergenic 30.0%
ACATAAGCACATTTATCAGA+GGG + Chr4:72045920-72045939 MsG0480022399.01.T01:CDS 30.0%
ACCGTTCATTTAAGAAGATT+TGG + Chr4:72046437-72046456 MsG0480022399.01.T01:intron 30.0%
AGAAATAAACTCAAAGGACA+AGG - Chr4:72047233-72047252 None:intergenic 30.0%
AGCATGTATAACTTCTTGAT+AGG - Chr4:72048323-72048342 None:intergenic 30.0%
ATTTACATGATAAACACGCA+AGG - Chr4:72046944-72046963 None:intergenic 30.0%
ATTTCAGCTGAAACAATGAT+TGG + Chr4:72046700-72046719 MsG0480022399.01.T01:CDS 30.0%
CAAAAACATTCAACTGTTCT+AGG - Chr4:72047894-72047913 None:intergenic 30.0%
CACATCAGAAATAAACTCAA+AGG - Chr4:72047239-72047258 None:intergenic 30.0%
CATCAAATAAAAAGGAATGG+AGG - Chr4:72046188-72046207 None:intergenic 30.0%
GGAAAATATTAAGGTTACGA+TGG + Chr4:72045114-72045133 MsG0480022399.01.T01:CDS 30.0%
GGCAGCCAAAAAATAAAATA+AGG + Chr4:72046493-72046512 MsG0480022399.01.T01:intron 30.0%
TAAATGTGCCCAGATTATTA+AGG + Chr4:72046738-72046757 MsG0480022399.01.T01:CDS 30.0%
TACTCTGTAGAAGATATTTC+AGG + Chr4:72045313-72045332 MsG0480022399.01.T01:intron 30.0%
TAGGCTCAATTGTCTATTTA+AGG - Chr4:72045068-72045087 None:intergenic 30.0%
TATAAGAGAAGGAGTTAGTT+AGG + Chr4:72045659-72045678 MsG0480022399.01.T01:intron 30.0%
TCAATAAAACCTCATTTGCA+AGG - Chr4:72046843-72046862 None:intergenic 30.0%
TTTACATGATAAACACGCAA+GGG - Chr4:72046943-72046962 None:intergenic 30.0%
! ATTTCTAACCATGTTTTCAG+GGG + Chr4:72047449-72047468 MsG0480022399.01.T01:intron 30.0%
! CATTTCTAACCATGTTTTCA+GGG + Chr4:72047448-72047467 MsG0480022399.01.T01:intron 30.0%
! GCTTTACAGTTTTCAGAAAT+GGG + Chr4:72045230-72045249 MsG0480022399.01.T01:intron 30.0%
! TGCTTTGTAATAGGTTTTTG+AGG + Chr4:72046976-72046995 MsG0480022399.01.T01:intron 30.0%
! TTGAAATGGCTCAGTTATTT+TGG - Chr4:72045479-72045498 None:intergenic 30.0%
!! AGTTCAGGTTTTATCTGTTA+GGG + Chr4:72048641-72048660 MsG0480022399.01.T01:three_prime_UTR 30.0%
!! ATGTTTCTTGTTATTGTTGG+TGG + Chr4:72047663-72047682 MsG0480022399.01.T01:intron 30.0%
!! TAGTTCAGGTTTTATCTGTT+AGG + Chr4:72048640-72048659 MsG0480022399.01.T01:three_prime_UTR 30.0%
!! TGAAATGGCTCAGTTATTTT+GGG - Chr4:72045478-72045497 None:intergenic 30.0%
!! TTGCTTTTGCTGATAGTAAT+CGG + Chr4:72048605-72048624 MsG0480022399.01.T01:three_prime_UTR 30.0%
!!! ATACTCCCACCATTATTTTT+TGG + Chr4:72046334-72046353 MsG0480022399.01.T01:intron 30.0%
!!! GTGCATATTTTTTCTGTGAT+TGG + Chr4:72047315-72047334 MsG0480022399.01.T01:intron 30.0%
AAGGTCTCATGCTTTGTAAT+AGG + Chr4:72046967-72046986 MsG0480022399.01.T01:intron 35.0%
AATCATTTCTACGATTGCCT+CGG + Chr4:72048419-72048438 MsG0480022399.01.T01:CDS 35.0%
ACACTTCCAAAAAATGAACG+TGG - Chr4:72046379-72046398 None:intergenic 35.0%
AGAAAATGGGTCAGATATTC+CGG - Chr4:72045443-72045462 None:intergenic 35.0%
AGCCGATTACACACATATTA+AGG - Chr4:72046563-72046582 None:intergenic 35.0%
AGTATCAGTGCATGATTGAT+AGG + Chr4:72045364-72045383 MsG0480022399.01.T01:intron 35.0%
AGTGGAGGAGGAAAATATTA+AGG + Chr4:72045105-72045124 MsG0480022399.01.T01:CDS 35.0%
AGTTAGGCTTAGAACTAGTT+AGG + Chr4:72045675-72045694 MsG0480022399.01.T01:intron 35.0%
ATCCAGATTATTGGAGTATG+TGG + Chr4:72047124-72047143 MsG0480022399.01.T01:intron 35.0%
ATCTGTGTGAAAAACCAAAC+TGG + Chr4:72045611-72045630 MsG0480022399.01.T01:intron 35.0%
CAAATGCAGGAATAACTTCA+TGG - Chr4:72045159-72045178 None:intergenic 35.0%
CACTTCCAAAAAATGAACGT+GGG - Chr4:72046378-72046397 None:intergenic 35.0%
CATGAAGTTATTCCTGCATT+TGG + Chr4:72045157-72045176 MsG0480022399.01.T01:CDS 35.0%
GAAATAGTTAATACTTGGGG+AGG - Chr4:72047093-72047112 None:intergenic 35.0%
GACATAAGCACATTTATCAG+AGG + Chr4:72045919-72045938 MsG0480022399.01.T01:CDS 35.0%
GGGAAAAAACTCATGGTTAT+TGG + Chr4:72047469-72047488 MsG0480022399.01.T01:CDS 35.0%
TAAAATTGAGCACACCTCAT+TGG - Chr4:72048151-72048170 None:intergenic 35.0%
TACCACTACAAAAACAAAGC+AGG - Chr4:72046088-72046107 None:intergenic 35.0%
TATTGGAACAACAAGTGAAG+TGG + Chr4:72047486-72047505 MsG0480022399.01.T01:CDS 35.0%
TCTGAAAACTGTAAAGCCTT+GGG - Chr4:72045227-72045246 None:intergenic 35.0%
TTCCAGAAGACAAGTAACAA+GGG - Chr4:72045988-72046007 None:intergenic 35.0%
! CATTCTATGCTTTTAAGCAC+AGG + Chr4:72048181-72048200 MsG0480022399.01.T01:intron 35.0%
! GCATTTCTAACCATGTTTTC+AGG + Chr4:72047447-72047466 MsG0480022399.01.T01:intron 35.0%
! GGCTTTACAGTTTTCAGAAA+TGG + Chr4:72045229-72045248 MsG0480022399.01.T01:intron 35.0%
! TGCTTTTGCTGATAGTAATC+GGG + Chr4:72048606-72048625 MsG0480022399.01.T01:three_prime_UTR 35.0%
! TGGCTTTACTCATAAAACAG+TGG + Chr4:72048012-72048031 MsG0480022399.01.T01:intron 35.0%
! TTAATATTTTCCTCCTCCAC+TGG - Chr4:72045106-72045125 None:intergenic 35.0%
! TTCTGAAAACTGTAAAGCCT+TGG - Chr4:72045228-72045247 None:intergenic 35.0%
!! TTTTCAGGGGAAAAAACTCA+TGG + Chr4:72047462-72047481 MsG0480022399.01.T01:intron 35.0%
!!! AACCTGCTTTGTTTTTGTAG+TGG + Chr4:72046083-72046102 MsG0480022399.01.T01:intron 35.0%
!!! AGTTTTTTCCCCTGAAAACA+TGG - Chr4:72047460-72047479 None:intergenic 35.0%
!!! ATACTCCCACGTTCATTTTT+TGG + Chr4:72046370-72046389 MsG0480022399.01.T01:intron 35.0%
!!! TGGTGGCTTTATGTTCTTTT+TGG + Chr4:72047680-72047699 MsG0480022399.01.T01:intron 35.0%
AACCAAAAACTACAGTGGTG+CGG + Chr4:72045006-72045025 MsG0480022399.01.T01:CDS 40.0%
AACTTCTTGATAGGCATCTG+AGG - Chr4:72048314-72048333 None:intergenic 40.0%
AAGAAAAGGTAAACCTCCCA+TGG - Chr4:72044968-72044987 None:intergenic 40.0%
AATTTACAGACTCCATGGCA+TGG + Chr4:72045881-72045900 MsG0480022399.01.T01:intron 40.0%
ATAAGCGTAACCTTCTGTAG+TGG + Chr4:72048701-72048720 MsG0480022399.01.T01:three_prime_UTR 40.0%
ATCATTTCTACGATTGCCTC+GGG + Chr4:72048420-72048439 MsG0480022399.01.T01:CDS 40.0%
CAAGGACAAACAGAAACCTT+TGG - Chr4:72047215-72047234 None:intergenic 40.0%
CAGCTGCACAAGAAAAGAAA+AGG - Chr4:72044982-72045001 None:intergenic 40.0%
CATAATTTCGCAGACACTAC+TGG + Chr4:72047169-72047188 MsG0480022399.01.T01:CDS 40.0%
CATACGTTGCATAGTACTTC+TGG + Chr4:72048548-72048567 MsG0480022399.01.T01:three_prime_UTR 40.0%
CTCTGAATTTACAGACTCCA+TGG + Chr4:72045876-72045895 MsG0480022399.01.T01:intron 40.0%
CTTCCAGAAGACAAGTAACA+AGG - Chr4:72045989-72046008 None:intergenic 40.0%
GAAAATGGGTCAGATATTCC+GGG - Chr4:72045442-72045461 None:intergenic 40.0%
GACAAGTGTCAGTCATCTAT+AGG + Chr4:72045739-72045758 MsG0480022399.01.T01:intron 40.0%
GAGCAAGTTAAAGTGAGCAA+AGG + Chr4:72045957-72045976 MsG0480022399.01.T01:CDS 40.0%
GATTACTGACCTTGCAAATG+AGG + Chr4:72046831-72046850 MsG0480022399.01.T01:intron 40.0%
GTTTGAAAAACGAGGACCAA+TGG - Chr4:72047150-72047169 None:intergenic 40.0%
TAAGCGTAACCTTCTGTAGT+GGG + Chr4:72048702-72048721 MsG0480022399.01.T01:three_prime_UTR 40.0%
TACATTATCCAATGCCTCTG+CGG - Chr4:72047938-72047957 None:intergenic 40.0%
TCCAGAAGACAAGTAACAAG+GGG - Chr4:72045987-72046006 None:intergenic 40.0%
TCGAGAACAATGACACTCAA+AGG - Chr4:72047016-72047035 None:intergenic 40.0%
TCTGCAGAAGCTATCTATTC+TGG + Chr4:72048377-72048396 MsG0480022399.01.T01:CDS 40.0%
TGAATTGAGCTGTACCAAAC+TGG + Chr4:72048677-72048696 MsG0480022399.01.T01:three_prime_UTR 40.0%
TGTAGTGGTAAAACCGCAAT+TGG + Chr4:72046098-72046117 MsG0480022399.01.T01:intron 40.0%
TTAGAACTAGTTAGGCTAGC+TGG + Chr4:72045683-72045702 MsG0480022399.01.T01:intron 40.0%
TTATTGGAGTATGTGGCCAT+TGG + Chr4:72047131-72047150 MsG0480022399.01.T01:CDS 40.0%
TTGGGGAGGAATTACAGATA+AGG - Chr4:72047079-72047098 None:intergenic 40.0%
TTGTTCTCGATGACATTGAG+AGG + Chr4:72047026-72047045 MsG0480022399.01.T01:CDS 40.0%
! AAAGGAATGGAGGGAGTATA+AGG - Chr4:72046178-72046197 None:intergenic 40.0%
! AACAAGTGAAGTGGACTTCT+TGG + Chr4:72047495-72047514 MsG0480022399.01.T01:CDS 40.0%
! AGTACTTCTGGTCAGTTTTG+AGG + Chr4:72048560-72048579 MsG0480022399.01.T01:three_prime_UTR 40.0%
! CTTGTTACTTGTCTTCTGGA+AGG + Chr4:72045987-72046006 MsG0480022399.01.T01:CDS 40.0%
! TCTATGCTTTTAAGCACAGG+AGG + Chr4:72048184-72048203 MsG0480022399.01.T01:intron 40.0%
!!! ACCACTGTAGTTTTTGGTTC+GGG - Chr4:72045005-72045024 None:intergenic 40.0%
!!! CACCACTGTAGTTTTTGGTT+CGG - Chr4:72045006-72045025 None:intergenic 40.0%
AAGCGTAACCTTCTGTAGTG+GGG + Chr4:72048703-72048722 MsG0480022399.01.T01:three_prime_UTR 45.0%
AGATGACCTCACTAAACCAG+TGG + Chr4:72045087-72045106 MsG0480022399.01.T01:CDS 45.0%
AGGTTACGCTTATGCCAGTT+TGG - Chr4:72048694-72048713 None:intergenic 45.0%
CTGGTTTAGTGAGGTCATCT+AGG - Chr4:72045087-72045106 None:intergenic 45.0%
CTTATTGAAATGGCAGCGCA+AGG + Chr4:72048341-72048360 MsG0480022399.01.T01:CDS 45.0%
GGACAAACAGAAACCTTTGG+AGG - Chr4:72047212-72047231 None:intergenic 45.0%
GGCCACATACTCCAATAATC+TGG - Chr4:72047129-72047148 None:intergenic 45.0%
GTGGACTTCTTGGATTCACT+TGG + Chr4:72047505-72047524 MsG0480022399.01.T01:CDS 45.0%
TCAGAGGGCAATGCTAATTG+CGG + Chr4:72045935-72045954 MsG0480022399.01.T01:CDS 45.0%
TCATTTCTACGATTGCCTCG+GGG + Chr4:72048421-72048440 MsG0480022399.01.T01:CDS 45.0%
TGTTGAATCCCCACTACAGA+AGG - Chr4:72048714-72048733 None:intergenic 45.0%
TTGCTGATAGTAATCGGGAC+AGG + Chr4:72048611-72048630 MsG0480022399.01.T01:three_prime_UTR 45.0%
! CAAACTGGCTCAGATATACC+GGG + Chr4:72045626-72045645 MsG0480022399.01.T01:intron 45.0%
! CCAAACTGGCTCAGATATAC+CGG + Chr4:72045625-72045644 MsG0480022399.01.T01:intron 45.0%
! TCCCCTTGTTACTTGTCTTC+TGG + Chr4:72045983-72046002 MsG0480022399.01.T01:CDS 45.0%
! TGAGGAGGCTTTGTCAATCA+TGG + Chr4:72048578-72048597 MsG0480022399.01.T01:three_prime_UTR 45.0%
! TTCCGCACCACTGTAGTTTT+TGG - Chr4:72045011-72045030 None:intergenic 45.0%
!! AGATATTGATTCTGCCGCAG+AGG + Chr4:72047921-72047940 MsG0480022399.01.T01:CDS 45.0%
!! ATAGGTTGATCTGCTACTGC+AGG + Chr4:72045757-72045776 MsG0480022399.01.T01:intron 45.0%
!! CACTACTGGTCTTGCTCAAA+CGG + Chr4:72047183-72047202 MsG0480022399.01.T01:CDS 45.0%
!! GGCATTTTGATAGAGCTCAC+AGG + Chr4:72047610-72047629 MsG0480022399.01.T01:intron 45.0%
!!! ACTTCTGGTCAGTTTTGAGG+AGG + Chr4:72048563-72048582 MsG0480022399.01.T01:three_prime_UTR 45.0%
ACCGCAATTGGAGCTACTGT+AGG + Chr4:72046110-72046129 MsG0480022399.01.T01:CDS 50.0%
ATGGGTCAGATATTCCGGGA+CGG - Chr4:72045438-72045457 None:intergenic 50.0%
CCGGTATATCTGAGCCAGTT+TGG - Chr4:72045628-72045647 None:intergenic 50.0%
CTAATCTGACAACATCCCCG+AGG - Chr4:72048439-72048458 None:intergenic 50.0%
CTGCTACTGCAGGTAACTGT+CGG + Chr4:72045767-72045786 MsG0480022399.01.T01:intron 50.0%
CTTCGCGTCATGTTTGCTCA+AGG - Chr4:72047569-72047588 None:intergenic 50.0%
GAGCAAACATGACGCGAAGA+AGG + Chr4:72047570-72047589 MsG0480022399.01.T01:CDS 50.0%
GCCCGAACCAAAAACTACAG+TGG + Chr4:72045001-72045020 MsG0480022399.01.T01:CDS 50.0%
GCCTACAGTAGCTCCAATTG+CGG - Chr4:72046114-72046133 None:intergenic 50.0%
GCGTCATGTTTGCTCAAGGT+GGG - Chr4:72047565-72047584 None:intergenic 50.0%
GGGACAGGTGTTTGTAGTTC+AGG + Chr4:72048626-72048645 MsG0480022399.01.T01:three_prime_UTR 50.0%
TACAGTGGTGCGGAACTTGA+AGG + Chr4:72045016-72045035 MsG0480022399.01.T01:CDS 50.0%
TCAGTGGAAGCTCCAAATGC+AGG - Chr4:72045172-72045191 None:intergenic 50.0%
TGACCTCACTAAACCAGTGG+AGG + Chr4:72045090-72045109 MsG0480022399.01.T01:CDS 50.0%
TTACAGACTCCATGGCATGG+TGG + Chr4:72045884-72045903 MsG0480022399.01.T01:intron 50.0%
! GACGGAGGGAGTATAAGAGA+AGG + Chr4:72045648-72045667 MsG0480022399.01.T01:intron 50.0%
! TGTCTTCTGGAAGGTTCTCG+TGG + Chr4:72045996-72046015 MsG0480022399.01.T01:CDS 50.0%
!! AGCGATGCAGGTACTGCTTT+TGG + Chr4:72045200-72045219 MsG0480022399.01.T01:intron 50.0%
!! AGGTACTGCTTTTGGTCCCA+AGG + Chr4:72045208-72045227 MsG0480022399.01.T01:intron 50.0%
ATAGACTACTCCCTCCGTCC+CGG + Chr4:72045421-72045440 MsG0480022399.01.T01:intron 55.0%
CCTCACTAAACCAGTGGAGG+AGG + Chr4:72045093-72045112 MsG0480022399.01.T01:CDS 55.0%
CGCGTCATGTTTGCTCAAGG+TGG - Chr4:72047566-72047585 None:intergenic 55.0%
CTCAGATATACCGGGACGGA+GGG + Chr4:72045634-72045653 MsG0480022399.01.T01:intron 55.0%
CTCTTATACTCCCTCCGTCC+CGG - Chr4:72045647-72045666 None:intergenic 55.0%
CTGACGATCTTGAGCGATGC+AGG + Chr4:72045188-72045207 MsG0480022399.01.T01:CDS 55.0%
CTGGCTCAGATATACCGGGA+CGG + Chr4:72045630-72045649 MsG0480022399.01.T01:intron 55.0%
GCATCGCTCAAGATCGTCAG+TGG - Chr4:72045188-72045207 None:intergenic 55.0%
GCTCAAACGGCTTCCTCCAA+AGG + Chr4:72047196-72047215 MsG0480022399.01.T01:CDS 55.0%
GTCAGATATTCCGGGACGGA+GGG - Chr4:72045434-72045453 None:intergenic 55.0%
! TGATTCTGCCGCAGAGGCAT+TGG + Chr4:72047927-72047946 MsG0480022399.01.T01:CDS 55.0%
! TTCTGGAAGGTTCTCGTGGC+AGG + Chr4:72046000-72046019 MsG0480022399.01.T01:CDS 55.0%
!! CCTCCTCCACTGGTTTAGTG+AGG - Chr4:72045096-72045115 None:intergenic 55.0%
CACCACACTCCACCATGCCA+TGG - Chr4:72045896-72045915 None:intergenic 60.0%
GCTCAGATATACCGGGACGG+AGG + Chr4:72045633-72045652 MsG0480022399.01.T01:intron 60.0%
GGTCAGATATTCCGGGACGG+AGG - Chr4:72045435-72045454 None:intergenic 60.0%
! CTCCATGGCATGGTGGAGTG+TGG + Chr4:72045891-72045910 MsG0480022399.01.T01:CDS 60.0%
ATGGCAGCGCAAGGTGCGCA+AGG + Chr4:72048350-72048369 MsG0480022399.01.T01:CDS 65.0%
GCAGCGCAAGGTGCGCAAGG+TGG + Chr4:72048353-72048372 MsG0480022399.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 72044953 72048855 72044953 ID=MsG0480022399.01;Name=MsG0480022399.01
Chr4 mRNA 72044953 72048855 72044953 ID=MsG0480022399.01.T01;Parent=MsG0480022399.01;Name=MsG0480022399.01.T01;_AED=0.49;_eAED=0.51;_QI=0|1|0.88|1|1|1|9|392|330
Chr4 exon 72044953 72045209 72044953 ID=MsG0480022399.01.T01:exon:4943;Parent=MsG0480022399.01.T01
Chr4 exon 72045890 72046021 72045890 ID=MsG0480022399.01.T01:exon:4944;Parent=MsG0480022399.01.T01
Chr4 exon 72046103 72046163 72046103 ID=MsG0480022399.01.T01:exon:4945;Parent=MsG0480022399.01.T01
Chr4 exon 72046700 72046759 72046700 ID=MsG0480022399.01.T01:exon:4946;Parent=MsG0480022399.01.T01
Chr4 exon 72046989 72047047 72046989 ID=MsG0480022399.01.T01:exon:4947;Parent=MsG0480022399.01.T01
Chr4 exon 72047130 72047217 72047130 ID=MsG0480022399.01.T01:exon:4948;Parent=MsG0480022399.01.T01
Chr4 exon 72047469 72047591 72047469 ID=MsG0480022399.01.T01:exon:4949;Parent=MsG0480022399.01.T01
Chr4 exon 72047889 72047954 72047889 ID=MsG0480022399.01.T01:exon:4950;Parent=MsG0480022399.01.T01
Chr4 exon 72048317 72048855 72048317 ID=MsG0480022399.01.T01:exon:4951;Parent=MsG0480022399.01.T01
Chr4 CDS 72044953 72045209 72044953 ID=MsG0480022399.01.T01:cds;Parent=MsG0480022399.01.T01
Chr4 CDS 72045890 72046021 72045890 ID=MsG0480022399.01.T01:cds;Parent=MsG0480022399.01.T01
Chr4 CDS 72046103 72046163 72046103 ID=MsG0480022399.01.T01:cds;Parent=MsG0480022399.01.T01
Chr4 CDS 72046700 72046759 72046700 ID=MsG0480022399.01.T01:cds;Parent=MsG0480022399.01.T01
Chr4 CDS 72046989 72047047 72046989 ID=MsG0480022399.01.T01:cds;Parent=MsG0480022399.01.T01
Chr4 CDS 72047130 72047217 72047130 ID=MsG0480022399.01.T01:cds;Parent=MsG0480022399.01.T01
Chr4 CDS 72047469 72047591 72047469 ID=MsG0480022399.01.T01:cds;Parent=MsG0480022399.01.T01
Chr4 CDS 72047889 72047954 72047889 ID=MsG0480022399.01.T01:cds;Parent=MsG0480022399.01.T01
Chr4 CDS 72048317 72048463 72048317 ID=MsG0480022399.01.T01:cds;Parent=MsG0480022399.01.T01
Chr4 three_prime_UTR 72048464 72048855 72048464 ID=MsG0480022399.01.T01:three_prime_utr;Parent=MsG0480022399.01.T01
Gene Sequence

>MsG0480022399.01.T01

ATGGTTTTTGAACCATGGGAGGTTTACCTTTTCTTTTCTTGTGCAGCTGCCCGAACCAAAAACTACAGTGGTGCGGAACTTGAAGGTGTTGTGAAAAGTGCTGTCTCATATGCCTTAAATAGACAATTGAGCCTAGATGACCTCACTAAACCAGTGGAGGAGGAAAATATTAAGGTTACGATGGATGACTTTTTGAATGCACTCCATGAAGTTATTCCTGCATTTGGAGCTTCCACTGACGATCTTGAGCGATGCAGACTCCATGGCATGGTGGAGTGTGGTGATCGACATAAGCACATTTATCAGAGGGCAATGCTAATTGCGGAGCAAGTTAAAGTGAGCAAAGGAAGTCCCCTTGTTACTTGTCTTCTGGAAGGTTCTCGTGGCAGTGGTAAAACCGCAATTGGAGCTACTGTAGGCATTGATAGTGACTTTGCATATGTCAAGATAATTTCAGCTGAAACAATGATTGGTCTACATGAGAGCACTAAATGTGCCCAGATTATTAAGGTTTTTGAGGATGCGTACAAGTCACCTTTGAGTGTCATTGTTCTCGATGACATTGAGAGATTATTGGAGTATGTGGCCATTGGTCCTCGTTTTTCAAACATAATTTCGCAGACACTACTGGTCTTGCTCAAACGGCTTCCTCCAAAGGGGAAAAAACTCATGGTTATTGGAACAACAAGTGAAGTGGACTTCTTGGATTCACTTGGATTTGTTGATACATTCTCTGTAACTTACAATGTTCCCACCTTGAGCAAACATGACGCGAAGAAGGTCCTAGAACAGTTGAATGTTTTTGCTGATGAAGATATTGATTCTGCCGCAGAGGCATTGGATAATATGCCTATCAAGAAGTTATACATGCTTATTGAAATGGCAGCGCAAGGTGCGCAAGGTGGATCTGCAGAAGCTATCTATTCTGGCAAAGAGAAGATTAATATCAATCATTTCTACGATTGCCTCGGGGATGTTGTCAGATTAGTATAG
Protein sequence

>MsG0480022399.01.T01

MVFEPWEVYLFFSCAAARTKNYSGAELEGVVKSAVSYALNRQLSLDDLTKPVEEENIKVTMDDFLNALHEVIPAFGASTDDLERCRLHGMVECGDRHKHIYQRAMLIAEQVKVSKGSPLVTCLLEGSRGSGKTAIGATVGIDSDFAYVKIISAETMIGLHESTKCAQIIKVFEDAYKSPLSVIVLDDIERLLEYVAIGPRFSNIISQTLLVLLKRLPPKGKKLMVIGTTSEVDFLDSLGFVDTFSVTYNVPTLSKHDAKKVLEQLNVFADEDIDSAAEALDNMPIKKLYMLIEMAAQGAQGGSAEAIYSGKEKININHFYDCLGDVVRLV*