AlfalfaGEDB | MsG0480022426.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022426.01

KEGG: -
GO: GO:0004672 GO:0005524 GO:0006468
PFAM: PF00069 PF07714

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022426.01.T01	XP_024636999.1	89.125	377	6	1	10	351	243	619	0	673

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022426.01.T01	P93604	47.059	357	144	5	15	328	276	630	6.56E-112	342

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022426.01.T01	A0A396I9I0	89.125	377	6	1	10	351	243	619	0.0	673

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048464.01	MsG0480022426.01	0.806672	7.343277e-50	4.009424e-47
MsG0080048683.01	MsG0480022426.01	0.800730	1.256454e-48	5.893875e-46
MsG0180000814.01	MsG0480022426.01	0.805282	1.439518e-49	7.584950e-47
MsG0180004765.01	MsG0480022426.01	0.819066	1.411273e-52	1.074873e-49
MsG0180005639.01	MsG0480022426.01	0.803546	3.311304e-49	1.668684e-46
MsG0180006054.01	MsG0480022426.01	0.809691	1.669549e-50	9.868868e-48
MsG0280006914.01	MsG0480022426.01	0.808139	3.585969e-50	2.034384e-47
MsG0280007118.01	MsG0480022426.01	0.803866	2.841865e-49	1.443957e-46
MsG0280008171.01	MsG0480022426.01	0.803154	3.990914e-49	1.990988e-46
MsG0280010517.01	MsG0480022426.01	0.804885	1.742474e-49	9.089583e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022426.01.T01	MTR_4g073230	86.234	385	13	2	2	351	231	610	0.0	660
MsG0480022426.01.T01	MTR_1g031320	55.584	385	131	3	7	351	236	620	7.57e-153	444
MsG0480022426.01.T01	MTR_1g028290	63.501	337	88	1	27	328	241	577	1.25e-152	442
MsG0480022426.01.T01	MTR_1g031460	55.496	373	126	3	19	351	235	607	3.59e-149	434
MsG0480022426.01.T01	MTR_1g031580	55.325	385	132	3	7	351	232	616	3.73e-148	431
MsG0480022426.01.T01	MTR_1g027150	58.177	373	116	5	18	351	201	572	2.31e-145	423
MsG0480022426.01.T01	MTR_1g027040	57.979	376	117	5	16	351	201	575	3.88e-144	420
MsG0480022426.01.T01	MTR_1g027060	61.739	345	93	4	45	351	176	519	5.65e-144	417
MsG0480022426.01.T01	MTR_1g028220	57.105	373	120	5	18	351	203	574	3.73e-143	417
MsG0480022426.01.T01	MTR_1g027820	53.500	400	132	8	4	351	174	571	8.10e-143	416

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022426.01.T01	AT5G38240	47.447	333	136	6	30	325	222	552	7.04e-96	296
MsG0480022426.01.T01	AT5G38240	47.447	333	136	6	30	325	268	598	1.35e-95	297
MsG0480022426.01.T01	AT5G38240	47.447	333	136	6	30	325	286	616	1.53e-95	297
MsG0480022426.01.T01	AT1G66910	43.045	381	160	7	22	350	282	657	7.44e-95	296
MsG0480022426.01.T01	AT1G66910	43.045	381	160	7	22	350	285	660	8.79e-95	296
MsG0480022426.01.T01	AT5G38250	42.391	368	168	7	24	350	256	620	3.81e-94	293
MsG0480022426.01.T01	AT5G38280	47.667	300	119	4	64	325	320	619	5.17e-94	294
MsG0480022426.01.T01	AT5G38280	47.667	300	119	4	64	325	320	619	5.27e-94	294
MsG0480022426.01.T01	AT1G66980	46.914	324	129	6	41	324	755	1075	5.98e-92	297
MsG0480022426.01.T01	AT1G66980	46.914	324	129	6	41	324	799	1119	7.58e-92	297

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Find 72 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTATCAAACGAAGTTC+AGG	0.376535	4:-72475208	MsG0480022426.01.T01:CDS
AAATCAGTTTAAGGAAGAAC+TGG	0.382385	4:-72475262	MsG0480022426.01.T01:CDS
AAGGAAAGTAACAGATGTCA+CGG	0.616045	4:-72474660	MsG0480022426.01.T01:CDS
AAGGTCCTAAATACTTCAAC+AGG	0.433839	4:-72475177	MsG0480022426.01.T01:CDS
AAGTATTGTGTCAATGACCA+CGG	0.667095	4:-72474798	MsG0480022426.01.T01:CDS
AAGTCAGATGTTTATAGTTA+TGG	0.308200	4:-72474710	MsG0480022426.01.T01:CDS
AAGTTTCTCGAAAATACCTC+TGG	0.535422	4:+72474749	None:intergenic
AATCAGTTTAAGGAAGAACT+GGG	0.583113	4:-72475261	MsG0480022426.01.T01:CDS
AATGCACCAAAGTCCCACGA+TGG	0.718706	4:+72474520	None:intergenic
ACACCCAAAATTTCTGATTT+TGG	0.143799	4:-72474848	MsG0480022426.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTATCAAACGAAGTTC+AGG	-	Chr4:72474732-72474751	MsG0480022426.01.T01:CDS	30.0%
AAATCAGTTTAAGGAAGAAC+TGG	-	Chr4:72474678-72474697	MsG0480022426.01.T01:CDS	30.0%
AATCAGTTTAAGGAAGAACT+GGG	-	Chr4:72474679-72474698	MsG0480022426.01.T01:CDS	30.0%
ACGAATCTCAAATCAGTTTA+AGG	-	Chr4:72474669-72474688	MsG0480022426.01.T01:CDS	30.0%
AGAAGGTTATAAATCCATTG+TGG	+	Chr4:72475332-72475351	None:intergenic	30.0%
CTAAATACTTCAACAGGAAA+TGG	-	Chr4:72474769-72474788	MsG0480022426.01.T01:CDS	30.0%
TCAAAAGAGAAAGTAATTGC+CGG	-	Chr4:72474379-72474398	MsG0480022426.01.T01:CDS	30.0%
TGACACAATACTTCTTTCTT+TGG	+	Chr4:72475135-72475154	None:intergenic	30.0%
TGGAAGTGAAGGATAATTAT+AGG	-	Chr4:72474436-72474455	MsG0480022426.01.T01:CDS	30.0%
TTTATAATACTATGTGCAGG+TGG	-	Chr4:72474493-72474512	MsG0480022426.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	72474346	72475616	72474346	ID=MsG0480022426.01;Name=MsG0480022426.01
Chr4	mRNA	72474346	72475616	72474346	ID=MsG0480022426.01.T01;Parent=MsG0480022426.01;Name=MsG0480022426.01.T01;_AED=0.50;_eAED=0.53;_QI=0\|0\|0\|1\|1\|1\|3\|0\|351
Chr4	exon	72475562	72475616	72475562	ID=MsG0480022426.01.T01:exon:5234;Parent=MsG0480022426.01.T01
Chr4	exon	72475103	72475450	72475103	ID=MsG0480022426.01.T01:exon:5233;Parent=MsG0480022426.01.T01
Chr4	exon	72474346	72474998	72474346	ID=MsG0480022426.01.T01:exon:5232;Parent=MsG0480022426.01.T01
Chr4	CDS	72475562	72475616	72475562	ID=MsG0480022426.01.T01:cds;Parent=MsG0480022426.01.T01
Chr4	CDS	72475103	72475450	72475103	ID=MsG0480022426.01.T01:cds;Parent=MsG0480022426.01.T01
Chr4	CDS	72474346	72474998	72474346	ID=MsG0480022426.01.T01:cds;Parent=MsG0480022426.01.T01

Gene Sequence

>MsG0480022426.01.T01

ATGAGTGACACATCTTTCAATTTTGCAGGTTTATCAAAAGAGAAAGTAATTGCCGGTGGAATCCTAGGCCCATTTTCTCTTATATTTGTTGGAGTCACAGTATATTGTATCTATGTAGCTTGCATTGAAAGAAAGAATTACATAAGAATCAAAAAGTTTTTGAAGGATTATAAAGCTCTTAAACCTTCCAGATACTCTTATGCTGATATAAAACGAATCTCAAATCAGTTTAAGGAAGAACTGGGGCAGGGAGCTTTTGGATCTGTCTTCAAAGGAAAACTATCAAACGAAGTTCAGGTAGCTGTTAAGGTCCTAAATACTTCAACAGGAAATGGCCAAGAGTTCATCAATGAAATGGAAGCTATGTGTCAAATTCATCACGTCAACGTGGCTCGCTTGGTTGGTTGGGATAGGCTTCAAAACATTTCTCTTGGTATAGCGAAGGGAATTGAGTATCTTCATCAAGGTTGTGATAAACGAATTCTCCATTTTGATATCAAACCTCATAACGTATTGCTAGACAACAATTTCACACCCAAAATTTCTGATTTTGGTTTGGCCAAACTTTGTTCCAAAGAAAGAAGTATTGTGTCAATGACCACGGCTAGGGGAACTTTGGGCTACATTGCGCCAGAGGTATTTTCGAGAAACTTTGGCAATGTATCCTATAAGTCAGATGTTTATAGTTATGGGATGCTGCTTCTTGAAATGGTTGGAGGAAGGAAAGTAACAGATGTCACGGATGAGAATAACAATCATGTTCACTATCCACAATGGATTTATAACCTTCTAGAGAACAACGAAGATATAAAAATCGATATAGAAGGAGAAGAAGACACTAGAATTGCAAAGAAGCTATCCATCGTGGGACTTTGGTGCATTCAGTGGCATCCTGTGAATCGTCCGACCATGAAGGTTGTGGTTCAAATGCTAGAAGGAAATGGTGAGAAGTTAGAGATGCCACCTAATCCTTTTGCATCTTCATCTGCAACTGCAACTAGAACTGGTGCTAGACGTCTCAACCTGGAGCTAGATGTCATCTCAGAAATAGAGTGA

Protein sequence

>MsG0480022426.01.T01

MSDTSFNFAGLSKEKVIAGGILGPFSLIFVGVTVYCIYVACIERKNYIRIKKFLKDYKALKPSRYSYADIKRISNQFKEELGQGAFGSVFKGKLSNEVQVAVKVLNTSTGNGQEFINEMEAMCQIHHVNVARLVGWDRLQNISLGIAKGIEYLHQGCDKRILHFDIKPHNVLLDNNFTPKISDFGLAKLCSKERSIVSMTTARGTLGYIAPEVFSRNFGNVSYKSDVYSYGMLLLEMVGGRKVTDVTDENNNHVHYPQWIYNLLENNEDIKIDIEGEEDTRIAKKLSIVGLWCIQWHPVNRPTMKVVVQMLEGNGEKLEMPPNPFASSSATATRTGARRLNLELDVISEIE*