AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004765.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004765.01.T01 MTR_1g086430 88.889 99 10 1 1 99 1 98 1.79e-45 143
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 12 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATGAGTGAAAATATTA+AGG 0.307429 1:+82448457 MsG0180004765.01.T01:CDS
GAGGAAGATGACACGGAAAA+AGG 0.323663 1:+82448587 MsG0180004765.01.T01:CDS
AAAATAGAGTTGTTGGTGTT+TGG 0.356784 1:+82448483 MsG0180004765.01.T01:CDS
AAGGAAGAAAATAGAGTTGT+TGG 0.384092 1:+82448476 MsG0180004765.01.T01:CDS
CTTGAAGATTTCGCTGAAGA+GGG 0.506298 1:+82448521 MsG0180004765.01.T01:CDS
AGATCAGTGCTAGCAAGAAA+AGG 0.526361 1:+82448321 MsG0180004765.01.T01:CDS
ACTTGAAGATTTCGCTGAAG+AGG 0.542202 1:+82448520 MsG0180004765.01.T01:CDS
TGCGTGAGACACGTGAGCGT+TGG 0.555623 1:+82448429 MsG0180004765.01.T01:CDS
TAACTGTGCTAATATTCTTG+AGG 0.568361 1:+82448568 MsG0180004765.01.T01:CDS
AAATCTTCAAGTTTAAACGT+TGG 0.594153 1:-82448509 None:intergenic
GAAGATTTCGCTGAAGAGGG+TGG 0.633634 1:+82448524 MsG0180004765.01.T01:CDS
TATTCTTGAGGAAGATGACA+CGG 0.661009 1:+82448580 MsG0180004765.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAATGAGTGAAAATATTA+AGG + Chr1:82448457-82448476 MsG0180004765.01.T01:CDS 20.0%
! TGAAGAAAAACAAAAAGAAG+AGG + Chr1:82448367-82448386 MsG0180004765.01.T01:CDS 25.0%
!! AAATCTTCAAGTTTAAACGT+TGG - Chr1:82448512-82448531 None:intergenic 25.0%
AAGGAAGAAAATAGAGTTGT+TGG + Chr1:82448476-82448495 MsG0180004765.01.T01:CDS 30.0%
TAACTGTGCTAATATTCTTG+AGG + Chr1:82448568-82448587 MsG0180004765.01.T01:CDS 30.0%
! AAAATAGAGTTGTTGGTGTT+TGG + Chr1:82448483-82448502 MsG0180004765.01.T01:CDS 30.0%
TATTCTTGAGGAAGATGACA+CGG + Chr1:82448580-82448599 MsG0180004765.01.T01:CDS 35.0%
ACTTGAAGATTTCGCTGAAG+AGG + Chr1:82448520-82448539 MsG0180004765.01.T01:CDS 40.0%
AGATCAGTGCTAGCAAGAAA+AGG + Chr1:82448321-82448340 MsG0180004765.01.T01:CDS 40.0%
CTTGAAGATTTCGCTGAAGA+GGG + Chr1:82448521-82448540 MsG0180004765.01.T01:CDS 40.0%
GAGGAAGATGACACGGAAAA+AGG + Chr1:82448587-82448606 MsG0180004765.01.T01:CDS 45.0%
GAAGATTTCGCTGAAGAGGG+TGG + Chr1:82448524-82448543 MsG0180004765.01.T01:CDS 50.0%
TGCGTGAGACACGTGAGCGT+TGG + Chr1:82448429-82448448 MsG0180004765.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 82448317 82448634 82448317 ID=MsG0180004765.01;Name=MsG0180004765.01
Chr1 mRNA 82448317 82448634 82448317 ID=MsG0180004765.01.T01;Parent=MsG0180004765.01;Name=MsG0180004765.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|105
Chr1 exon 82448317 82448634 82448317 ID=MsG0180004765.01.T01:exon:27277;Parent=MsG0180004765.01.T01
Chr1 CDS 82448317 82448634 82448317 ID=MsG0180004765.01.T01:cds;Parent=MsG0180004765.01.T01
Gene Sequence

>MsG0180004765.01.T01

ATGGAGATCAGTGCTAGCAAGAAAAGGAAAATCTGCAACGAAGAATTAGATGAAGAAAAACAAAAAGAAGAGGAAGAGAAAATAGAGACATTTTTTGCTCTAGTGAGAAGCATGCGTGAGACACGTGAGCGTTGGAAGAACAAAATGAGTGAAAATATTAAGGAAGAAAATAGAGTTGTTGGTGTTTGGAAGCCAACGTTTAAACTTGAAGATTTCGCTGAAGAGGGTGGTGCATCTAAGAACAATAATATTAACTGTGCTAATATTCTTGAGGAAGATGACACGGAAAAAGGTATTAACTTGAAGCTCTCACTTTGA

Protein sequence

>MsG0180004765.01.T01

MEISASKKRKICNEELDEEKQKEEEEKIETFFALVRSMRETRERWKNKMSENIKEENRVVGVWKPTFKLEDFAEEGGASKNNNINCANILEEDDTEKGINLKLSL*