AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022444.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022444.01.T01 MTR_4g073100 80.150 267 13 2 1 227 1 267 1.29e-150 419
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022444.01.T01 AT2G16770 52.874 261 75 5 1 226 1 248 8.10e-83 247
MsG0480022444.01.T01 AT2G16770 52.874 261 75 5 1 226 1 248 8.10e-83 247
MsG0480022444.01.T01 AT4G35040 53.184 267 77 7 1 226 1 260 2.37e-80 241
MsG0480022444.01.T01 AT4G35040 53.184 267 77 7 1 226 1 260 2.37e-80 241
MsG0480022444.01.T01 AT4G35040 53.184 267 77 7 1 226 1 260 2.37e-80 241
MsG0480022444.01.T01 AT4G35040 53.184 267 77 7 1 226 1 260 2.37e-80 241
MsG0480022444.01.T01 AT3G51960 52.756 127 53 3 39 164 13 133 3.62e-41 139
MsG0480022444.01.T01 AT3G51960 52.756 127 53 3 39 164 52 172 9.80e-41 139
MsG0480022444.01.T01 AT3G51960 50.394 127 55 2 39 164 52 171 2.77e-39 135

Find 100 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTCACAAATTTATATTTC+TGG 0.109918 4:-72599590 MsG0480022444.01.T01:three_prime_UTR
GGGAGGATTGAAGGGGAAAT+TGG 0.174039 4:-72601176 MsG0480022444.01.T01:CDS
TCCTATGCAATTAAGTTTCA+TGG 0.237006 4:-72599753 MsG0480022444.01.T01:three_prime_UTR
GTGTTTGTTGGTTGATATTA+GGG 0.255056 4:-72601197 MsG0480022444.01.T01:CDS
CCCAACCGGTCGCTTCTTAC+TGG 0.266035 4:+72601372 None:intergenic
GATCCTTGAGCCCCAAATTC+TGG 0.270466 4:+72600952 None:intergenic
AATATTAGTAGTTGATAAAT+AGG 0.291883 4:-72599842 MsG0480022444.01.T01:three_prime_UTR
GTGTATGGGAGGCCAGAATT+TGG 0.299733 4:-72600964 MsG0480022444.01.T01:CDS
AGTGTTTGTTGGTTGATATT+AGG 0.302818 4:-72601198 MsG0480022444.01.T01:CDS
GTGAGTGAAAACAATGAAAT+TGG 0.307457 4:-72599476 MsG0480022444.01.T01:three_prime_UTR
TGTATGGGAGGCCAGAATTT+GGG 0.316343 4:-72600963 MsG0480022444.01.T01:CDS
GTTGCAAGGGCAGGCTGCTT+TGG 0.335855 4:-72601242 MsG0480022444.01.T01:CDS
TGCAGCTGGTAACAAGTTAT+TGG 0.342321 4:+72599958 None:intergenic
ATATATTATTCATCCGGCTT+TGG 0.362005 4:+72600025 None:intergenic
CATGTCCTTGCTGCTGAAAC+AGG 0.362256 4:-72599785 MsG0480022444.01.T01:three_prime_UTR
GTTTGAAATTCAGCCATGTT+TGG 0.367715 4:+72601589 None:intergenic
CTCAAGGATCTTCGTGGTTG+TGG 0.376487 4:-72600939 MsG0480022444.01.T01:CDS
TGCGCAGCAGAGGGTCAAAA+AGG 0.378364 4:+72599895 None:intergenic
GACCCTCTGCTGCGCAATGA+TGG 0.389317 4:-72599888 MsG0480022444.01.T01:three_prime_UTR
CAATTAAGTTTCATGGTGTT+TGG 0.389488 4:-72599746 MsG0480022444.01.T01:three_prime_UTR
GGAGGATTGAAGGGGAAATT+GGG 0.395641 4:-72601175 MsG0480022444.01.T01:CDS
TGATATTAGGGGAGGATTGA+AGG 0.403249 4:-72601185 MsG0480022444.01.T01:CDS
ATTTCAAACAAGTGGTTCAA+TGG 0.405614 4:-72601575 MsG0480022444.01.T01:CDS
ACAATTATATATTATTCATC+CGG 0.412039 4:+72600019 None:intergenic
GTTTGAGAGTCTCCAGTGTA+TGG 0.417641 4:-72600979 MsG0480022444.01.T01:CDS
TGCGAGGTTGAAGTGTTTGT+TGG 0.420725 4:-72601209 MsG0480022444.01.T01:CDS
GCAAGGGCAGGCTGCTTTGG+AGG 0.426440 4:-72601239 MsG0480022444.01.T01:CDS
GGCTGCGTCGTTGGAGGATG+AGG 0.428168 4:-72601305 MsG0480022444.01.T01:CDS
TATTTCTGGATTACAGAACT+TGG 0.429567 4:-72599576 MsG0480022444.01.T01:three_prime_UTR
GATCTTCGTGGTTGTGGAGG+TGG 0.440100 4:-72600933 MsG0480022444.01.T01:CDS
GAAATTGGTTATGTGGTTGC+CGG 0.450547 4:-72599461 None:intergenic
ACCGCCTCTTTATTCCCAAC+CGG 0.455765 4:+72601358 None:intergenic
TCGACCTTTCGTTGTCTCCA+TGG 0.463517 4:-72599993 MsG0480022444.01.T01:three_prime_UTR
ATTACAGAACTTGGGGAAGA+AGG 0.466762 4:-72599567 MsG0480022444.01.T01:three_prime_UTR
ATTTCTGGATTACAGAACTT+GGG 0.474565 4:-72599575 MsG0480022444.01.T01:three_prime_UTR
GAAAGCTAGGGCTGCGTCGT+TGG 0.478279 4:-72601314 MsG0480022444.01.T01:CDS
TTACAGAACTTGGGGAAGAA+GGG 0.483404 4:-72599566 MsG0480022444.01.T01:three_prime_UTR
GGGTCTCGTGCAGCCAAAGC+CGG 0.486937 4:-72600038 MsG0480022444.01.T01:CDS
AGCTAGGGCTGCGTCGTTGG+AGG 0.489293 4:-72601311 MsG0480022444.01.T01:CDS
CTGCTGAAGATGATCAGGTT+GGG 0.489651 4:-72601429 MsG0480022444.01.T01:CDS
AGGCCAGAATTTGGGGCTCA+AGG 0.491612 4:-72600955 MsG0480022444.01.T01:CDS
CAGTACTTTGTGCTGTGGAC+AGG 0.494865 4:-72599529 MsG0480022444.01.T01:three_prime_UTR
ATCTTCAGCAGGGACAATCT+TGG 0.499105 4:+72601441 None:intergenic
AGCAAGGACATGCTGACTAC+AGG 0.500075 4:+72599796 None:intergenic
TTGGGGCTCAAGGATCTTCG+TGG 0.500226 4:-72600945 MsG0480022444.01.T01:CDS
CCATCGAATCCATCCTCATT+CGG 0.507560 4:+72601008 None:intergenic
TGTCCCTGCTGAAGATGATC+AGG 0.516014 4:-72601434 MsG0480022444.01.T01:CDS
CCAGTAAGAAGCGACCGGTT+GGG 0.517346 4:-72601372 MsG0480022444.01.T01:CDS
ATCGTGAGAAGAAGAAAGCT+AGG 0.518063 4:-72601327 MsG0480022444.01.T01:CDS
GCTGCAGCATCAGTTATCCT+TGG 0.520134 4:-72599941 MsG0480022444.01.T01:three_prime_UTR
AAACAATGAAATTGGTTATG+TGG 0.520264 4:-72599468 MsG0480022444.01.T01:three_prime_UTR
CTTTGGAGGCCGAAGTTGCG+AGG 0.525689 4:-72601225 MsG0480022444.01.T01:CDS
CCAACCTGATCATCTTCAGC+AGG 0.525807 4:+72601430 None:intergenic
GATGAGGAAGTTGCAAGGGC+AGG 0.525833 4:-72601251 MsG0480022444.01.T01:CDS
TCCAGTAAGAAGCGACCGGT+TGG 0.526553 4:-72601373 MsG0480022444.01.T01:CDS
AGGATGAGGTCATGAAATTG+AGG 0.534541 4:-72601291 MsG0480022444.01.T01:CDS
TTGTTGGTTGATATTAGGGG+AGG 0.537193 4:-72601193 MsG0480022444.01.T01:CDS
ATATTAGGGGAGGATTGAAG+GGG 0.540235 4:-72601183 MsG0480022444.01.T01:CDS
GCTGGTAACAAGTTATTGGC+AGG 0.542987 4:+72599962 None:intergenic
AGGATAACTGATGCTGCAGC+TGG 0.548774 4:+72599944 None:intergenic
CATCTCAGTACTTTGTGCTG+TGG 0.548850 4:-72599534 MsG0480022444.01.T01:three_prime_UTR
AATTCTGGCCTCCCATACAC+TGG 0.551814 4:+72600967 None:intergenic
ATGGCTGAATTTCAAACAAG+TGG 0.554218 4:-72601583 MsG0480022444.01.T01:CDS
GTGGCGTCGCCGAATGAGGA+TGG 0.554803 4:-72601017 MsG0480022444.01.T01:CDS
TATCAACTACTAATATTCCA+TGG 0.556415 4:+72599848 None:intergenic
GACCAGGTGGGTTACACGTG+TGG 0.559695 4:+72601503 None:intergenic
GATATTAGGGGAGGATTGAA+GGG 0.560294 4:-72601184 MsG0480022444.01.T01:CDS
TACAGAACTTGGGGAAGAAG+GGG 0.568426 4:-72599565 MsG0480022444.01.T01:three_prime_UTR
ACACTTCAACCTCGCAACTT+CGG 0.570082 4:+72601216 None:intergenic
TGTTTGTTGGTTGATATTAG+GGG 0.574670 4:-72601196 MsG0480022444.01.T01:CDS
TCCTTGCTGCTGAAACAGGT+TGG 0.577744 4:-72599781 MsG0480022444.01.T01:three_prime_UTR
GGATGAGGTCATGAAATTGA+GGG 0.578672 4:-72601290 MsG0480022444.01.T01:CDS
ACACGTGTGGGTATGAGTAC+AGG 0.580524 4:+72601516 None:intergenic
GAAGTCCAGTAAGAAGCGAC+CGG 0.585566 4:-72601377 MsG0480022444.01.T01:CDS
TTTGAGAGTCTCCAGTGTAT+GGG 0.588557 4:-72600978 MsG0480022444.01.T01:CDS
ATGAACTAGTTAAACTAGCA+TGG 0.593729 4:-72599715 MsG0480022444.01.T01:three_prime_UTR
TTTCTGGATTACAGAACTTG+GGG 0.593836 4:-72599574 MsG0480022444.01.T01:three_prime_UTR
GTATGGGAGGCCAGAATTTG+GGG 0.595109 4:-72600962 MsG0480022444.01.T01:CDS
ATTTGATGAGGAAGTTGCAA+GGG 0.596253 4:-72601255 MsG0480022444.01.T01:CDS
CCGAATGAGGATGGATTCGA+TGG 0.597469 4:-72601008 MsG0480022444.01.T01:CDS
TGAAGTGGCGTCGCCGAATG+AGG 0.598815 4:-72601021 MsG0480022444.01.T01:CDS
AAGGATCTTCGTGGTTGTGG+AGG 0.602836 4:-72600936 MsG0480022444.01.T01:CDS
CATTTGATGAGGAAGTTGCA+AGG 0.604562 4:-72601256 MsG0480022444.01.T01:CDS
ACCAACCTGTTTCAGCAGCA+AGG 0.604863 4:+72599780 None:intergenic
AAACCATCATTGCGCAGCAG+AGG 0.606548 4:+72599885 None:intergenic
ACCCACACGTGTAACCCACC+TGG 0.607124 4:-72601505 MsG0480022444.01.T01:CDS
ACCAGGTGGGTTACACGTGT+GGG 0.610384 4:+72601504 None:intergenic
TCGTGAGAAGAAGAAAGCTA+GGG 0.612179 4:-72601326 MsG0480022444.01.T01:CDS
TGGGAAGATTGCTGCTGAAG+TGG 0.614233 4:-72601036 MsG0480022444.01.T01:CDS
ACCATGAAACTTAATTGCAT+AGG 0.615918 4:+72599752 None:intergenic
CTTTGAATCAGCATTTGATG+AGG 0.616238 4:-72601267 MsG0480022444.01.T01:CDS
AACCATCATTGCGCAGCAGA+GGG 0.616899 4:+72599886 None:intergenic
CCTGCTGAAGATGATCAGGT+TGG 0.627520 4:-72601430 MsG0480022444.01.T01:CDS
GAGAGTCTCCAGTGTATGGG+AGG 0.629740 4:-72600975 MsG0480022444.01.T01:CDS
GCGACCGGTTGGGAATAAAG+AGG 0.649541 4:-72601362 MsG0480022444.01.T01:CDS
ATTACCATGGAGACAACGAA+AGG 0.677693 4:+72599989 None:intergenic
CAACCTGATCATCTTCAGCA+GGG 0.684116 4:+72601431 None:intergenic
ACCGGTTGGGAATAAAGAGG+CGG 0.690479 4:-72601359 MsG0480022444.01.T01:CDS
ATTGGCAGGATATATTACCA+TGG 0.699557 4:+72599976 None:intergenic
GAATTGACATTAGACAACGT+CGG 0.759673 4:+72600909 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATTATATATTATTCATC+CGG + Chr4:72601083-72601102 None:intergenic 15.0%
!!! AATATTAGTAGTTGATAAAT+AGG - Chr4:72601257-72601276 MsG0480022444.01.T01:CDS 15.0%
!! ACATAATGCAAAAACAAATA+AGG + Chr4:72600500-72600519 None:intergenic 20.0%
!! AGTTCACAAATTTATATTTC+TGG - Chr4:72601509-72601528 MsG0480022444.01.T01:CDS 20.0%
!! CATAATGCAAAAACAAATAA+GGG + Chr4:72600499-72600518 None:intergenic 20.0%
!!! TAACAGATCTTTATTTAACA+GGG - Chr4:72601038-72601057 MsG0480022444.01.T01:CDS 20.0%
!!! TTTTTTTGATGAATTGTTGA+AGG - Chr4:72599551-72599570 MsG0480022444.01.T01:three_prime_UTR 20.0%
! AAACAATGAAATTGGTTATG+TGG - Chr4:72601631-72601650 MsG0480022444.01.T01:CDS 25.0%
! GTTGGAGAAATTGAAATTTA+AGG - Chr4:72600820-72600839 MsG0480022444.01.T01:intron 25.0%
! TATATGTATGAGTTAACAAG+TGG - Chr4:72600768-72600787 MsG0480022444.01.T01:intron 25.0%
! TATCAACTACTAATATTCCA+TGG + Chr4:72601254-72601273 None:intergenic 25.0%
!! AACAGATCTTTATTTAACAG+GGG - Chr4:72601039-72601058 MsG0480022444.01.T01:CDS 25.0%
!! CTAACAGATCTTTATTTAAC+AGG - Chr4:72601037-72601056 MsG0480022444.01.T01:CDS 25.0%
!! TCATACATATATAAAGCATC+TGG + Chr4:72600759-72600778 None:intergenic 25.0%
!!! TAATGAGATGATGTTTTAAG+TGG - Chr4:72601470-72601489 MsG0480022444.01.T01:CDS 25.0%
AAACCATGACAAAAAATGCT+AGG + Chr4:72600354-72600373 None:intergenic 30.0%
AACAAAAGCCTTTCCATATA+AGG + Chr4:72600558-72600577 None:intergenic 30.0%
ACCATGAAACTTAATTGCAT+AGG + Chr4:72601350-72601369 None:intergenic 30.0%
ACTCTGGTGTAATTACTTTA+GGG - Chr4:72600968-72600987 MsG0480022444.01.T01:CDS 30.0%
ATATATTATTCATCCGGCTT+TGG + Chr4:72601077-72601096 None:intergenic 30.0%
ATGAACTAGTTAAACTAGCA+TGG - Chr4:72601384-72601403 MsG0480022444.01.T01:CDS 30.0%
ATTTCAAACAAGTGGTTCAA+TGG - Chr4:72599524-72599543 MsG0480022444.01.T01:three_prime_UTR 30.0%
CAATTAAGTTTCATGGTGTT+TGG - Chr4:72601353-72601372 MsG0480022444.01.T01:CDS 30.0%
CTAACAAGAACTCTATAAGT+TGG + Chr4:72600726-72600745 None:intergenic 30.0%
GTGAGTGAAAACAATGAAAT+TGG - Chr4:72601623-72601642 MsG0480022444.01.T01:CDS 30.0%
TCCTATGCAATTAAGTTTCA+TGG - Chr4:72601346-72601365 MsG0480022444.01.T01:CDS 30.0%
TGGAAGATCTTGTCATAATT+TGG + Chr4:72600796-72600815 None:intergenic 30.0%
! ACAGATCTTTATTTAACAGG+GGG - Chr4:72601040-72601059 MsG0480022444.01.T01:CDS 30.0%
! AGTGTTTGTTGGTTGATATT+AGG - Chr4:72599901-72599920 MsG0480022444.01.T01:three_prime_UTR 30.0%
! ATTTCTGGATTACAGAACTT+GGG - Chr4:72601524-72601543 MsG0480022444.01.T01:CDS 30.0%
! GATCTGTTAGACCATTTTAA+AGG + Chr4:72601027-72601046 None:intergenic 30.0%
! GATTTTCAAAAGTTACTCCA+AGG + Chr4:72601178-72601197 None:intergenic 30.0%
! GTGTTTGTTGGTTGATATTA+GGG - Chr4:72599902-72599921 MsG0480022444.01.T01:three_prime_UTR 30.0%
! TGTTTGTTGGTTGATATTAG+GGG - Chr4:72599903-72599922 MsG0480022444.01.T01:three_prime_UTR 30.0%
! TTGCTTCTCATCCTTTAAAA+TGG - Chr4:72601013-72601032 MsG0480022444.01.T01:CDS 30.0%
!! AGAGTTCTTGTTAGTTTGTA+TGG - Chr4:72600732-72600751 MsG0480022444.01.T01:intron 30.0%
!! TATTTCTGGATTACAGAACT+TGG - Chr4:72601523-72601542 MsG0480022444.01.T01:CDS 30.0%
!!! AGCATTTTTTGTCATGGTTT+GGG - Chr4:72600354-72600373 MsG0480022444.01.T01:intron 30.0%
!!! TAGCATTTTTTGTCATGGTT+TGG - Chr4:72600353-72600372 MsG0480022444.01.T01:intron 30.0%
ACGAGACAAACAATCATCAT+TGG + Chr4:72600382-72600401 None:intergenic 35.0%
AGAGTCAATCACAATTGCTT+TGG + Chr4:72600953-72600972 None:intergenic 35.0%
ATGCACATACTCTACTAGAA+AGG - Chr4:72600249-72600268 MsG0480022444.01.T01:intron 35.0%
ATGGCTGAATTTCAAACAAG+TGG - Chr4:72599516-72599535 MsG0480022444.01.T01:three_prime_UTR 35.0%
ATTGGCAGGATATATTACCA+TGG + Chr4:72601126-72601145 None:intergenic 35.0%
ATTTGATGAGGAAGTTGCAA+GGG - Chr4:72599844-72599863 MsG0480022444.01.T01:three_prime_UTR 35.0%
CAAGCTTCCAGTAAAAGAAA+AGG - Chr4:72600214-72600233 MsG0480022444.01.T01:intron 35.0%
CTTTGAATCAGCATTTGATG+AGG - Chr4:72599832-72599851 MsG0480022444.01.T01:three_prime_UTR 35.0%
GAATTGACATTAGACAACGT+CGG + Chr4:72600193-72600212 None:intergenic 35.0%
GACTCTGGTGTAATTACTTT+AGG - Chr4:72600967-72600986 MsG0480022444.01.T01:CDS 35.0%
TGCACATACTCTACTAGAAA+GGG - Chr4:72600250-72600269 MsG0480022444.01.T01:intron 35.0%
TTAAAATCCGGAATGAGCAA+GGG + Chr4:72600672-72600691 None:intergenic 35.0%
TTATGACAAGATCTTCCAGT+AGG - Chr4:72600798-72600817 MsG0480022444.01.T01:intron 35.0%
TTTCAATTTCTCCAACCTAC+TGG + Chr4:72600816-72600835 None:intergenic 35.0%
TTTCTGGATTACAGAACTTG+GGG - Chr4:72601525-72601544 MsG0480022444.01.T01:CDS 35.0%
! AAAGCAATTGTGATTGACTC+TGG - Chr4:72600952-72600971 MsG0480022444.01.T01:CDS 35.0%
! CAGATCTTTATTTAACAGGG+GGG - Chr4:72601041-72601060 MsG0480022444.01.T01:intron 35.0%
! GTCATCACCTTTTCTTTTAC+TGG + Chr4:72600224-72600243 None:intergenic 35.0%
! GTTTGAAATTCAGCCATGTT+TGG + Chr4:72599513-72599532 None:intergenic 35.0%
! TGCTCATTCCGGATTTTAAA+GGG - Chr4:72600673-72600692 MsG0480022444.01.T01:intron 35.0%
! TTGCTCATTCCGGATTTTAA+AGG - Chr4:72600672-72600691 MsG0480022444.01.T01:intron 35.0%
! TTTAAAATCCGGAATGAGCA+AGG + Chr4:72600673-72600692 None:intergenic 35.0%
!! ACTAGACTCCCTTTAAAATC+CGG + Chr4:72600684-72600703 None:intergenic 35.0%
!! TTTTGATGGCTATTCTACCA+TGG - Chr4:72601234-72601253 MsG0480022444.01.T01:CDS 35.0%
!!! TCTCAACTTTTTCGTACAGT+GGG + Chr4:72600881-72600900 None:intergenic 35.0%
!!! TCTCCTAGCATTTTTTGTCA+TGG - Chr4:72600348-72600367 MsG0480022444.01.T01:intron 35.0%
AATGTTACCCTTGCTCATTC+CGG - Chr4:72600662-72600681 MsG0480022444.01.T01:intron 40.0%
AGGATGAGGTCATGAAATTG+AGG - Chr4:72599808-72599827 MsG0480022444.01.T01:three_prime_UTR 40.0%
ATATTAGGGGAGGATTGAAG+GGG - Chr4:72599916-72599935 MsG0480022444.01.T01:three_prime_UTR 40.0%
ATCGTGAGAAGAAGAAAGCT+AGG - Chr4:72599772-72599791 MsG0480022444.01.T01:three_prime_UTR 40.0%
ATTACAGAACTTGGGGAAGA+AGG - Chr4:72601532-72601551 MsG0480022444.01.T01:CDS 40.0%
ATTACCATGGAGACAACGAA+AGG + Chr4:72601113-72601132 None:intergenic 40.0%
CATCACACTGCACATTACAA+GGG + Chr4:72600018-72600037 None:intergenic 40.0%
CATTTGATGAGGAAGTTGCA+AGG - Chr4:72599843-72599862 MsG0480022444.01.T01:three_prime_UTR 40.0%
GATATTAGGGGAGGATTGAA+GGG - Chr4:72599915-72599934 MsG0480022444.01.T01:three_prime_UTR 40.0%
GGATGAGGTCATGAAATTGA+GGG - Chr4:72599809-72599828 MsG0480022444.01.T01:three_prime_UTR 40.0%
GTAATGTGCAGTGTGATGAT+CGG - Chr4:72600020-72600039 MsG0480022444.01.T01:exon 40.0%
GTGTGAGTGTGTGAAAAATC+AGG + Chr4:72599621-72599640 None:intergenic 40.0%
TAATGTGCAGTGTGATGATC+GGG - Chr4:72600021-72600040 MsG0480022444.01.T01:exon 40.0%
TCATCACACTGCACATTACA+AGG + Chr4:72600019-72600038 None:intergenic 40.0%
TCGTGAGAAGAAGAAAGCTA+GGG - Chr4:72599773-72599792 MsG0480022444.01.T01:three_prime_UTR 40.0%
TGACTCTGCCTTATATGGAA+AGG - Chr4:72600547-72600566 MsG0480022444.01.T01:intron 40.0%
TGATATTAGGGGAGGATTGA+AGG - Chr4:72599914-72599933 MsG0480022444.01.T01:three_prime_UTR 40.0%
TGCAGCTGGTAACAAGTTAT+TGG + Chr4:72601144-72601163 None:intergenic 40.0%
TTACAGAACTTGGGGAAGAA+GGG - Chr4:72601533-72601552 MsG0480022444.01.T01:CDS 40.0%
TTTGAGAGTCTCCAGTGTAT+GGG - Chr4:72600121-72600140 MsG0480022444.01.T01:intron 40.0%
! TTGTTGGTTGATATTAGGGG+AGG - Chr4:72599906-72599925 MsG0480022444.01.T01:three_prime_UTR 40.0%
!!! GTCTCAACTTTTTCGTACAG+TGG + Chr4:72600882-72600901 None:intergenic 40.0%
ACACTTCAACCTCGCAACTT+CGG + Chr4:72599886-72599905 None:intergenic 45.0%
ACATTCGCGGGTTTCTGATA+CGG + Chr4:72599956-72599975 None:intergenic 45.0%
ATCTTCAGCAGGGACAATCT+TGG + Chr4:72599661-72599680 None:intergenic 45.0%
CAACCTGATCATCTTCAGCA+GGG + Chr4:72599671-72599690 None:intergenic 45.0%
CATATTGCCTTCGTCCAGAT+GGG - Chr4:72600044-72600063 MsG0480022444.01.T01:intron 45.0%
CATCTCAGTACTTTGTGCTG+TGG - Chr4:72601565-72601584 MsG0480022444.01.T01:CDS 45.0%
CCATCGAATCCATCCTCATT+CGG + Chr4:72600094-72600113 None:intergenic 45.0%
CTGCTGAAGATGATCAGGTT+GGG - Chr4:72599670-72599689 MsG0480022444.01.T01:three_prime_UTR 45.0%
GACAAGATCTTCCAGTAGGT+TGG - Chr4:72600802-72600821 MsG0480022444.01.T01:intron 45.0%
GCTGGTAACAAGTTATTGGC+AGG + Chr4:72601140-72601159 None:intergenic 45.0%
GGAGGATTGAAGGGGAAATT+GGG - Chr4:72599924-72599943 MsG0480022444.01.T01:three_prime_UTR 45.0%
GTGAAAAATCAGGACCAGGT+GGG + Chr4:72599611-72599630 None:intergenic 45.0%
GTGTGTGAAAAATCAGGACC+AGG + Chr4:72599615-72599634 None:intergenic 45.0%
GTTTGAGAGTCTCCAGTGTA+TGG - Chr4:72600120-72600139 MsG0480022444.01.T01:intron 45.0%
TACAGAACTTGGGGAAGAAG+GGG - Chr4:72601534-72601553 MsG0480022444.01.T01:CDS 45.0%
TCAAGTTCACCGTCTTCCAT+CGG + Chr4:72599462-72599481 None:intergenic 45.0%
TGTATGGGAGGCCAGAATTT+GGG - Chr4:72600136-72600155 MsG0480022444.01.T01:intron 45.0%
TGTGAAAAATCAGGACCAGG+TGG + Chr4:72599612-72599631 None:intergenic 45.0%
! GTAGCTGACTCTGCCTTATA+TGG - Chr4:72600542-72600561 MsG0480022444.01.T01:intron 45.0%
! TGCGAGGTTGAAGTGTTTGT+TGG - Chr4:72599890-72599909 MsG0480022444.01.T01:three_prime_UTR 45.0%
!!! CGTGTTTTCGAGTCCAAACA+TGG - Chr4:72599497-72599516 MsG0480022444.01.T01:three_prime_UTR 45.0%
!!! CTGCGCAATGATGGTTTTGA+TGG - Chr4:72601220-72601239 MsG0480022444.01.T01:CDS 45.0%
AAACCATCATTGCGCAGCAG+AGG + Chr4:72601217-72601236 None:intergenic 50.0%
AACCATCATTGCGCAGCAGA+GGG + Chr4:72601216-72601235 None:intergenic 50.0%
AAGGATCTTCGTGGTTGTGG+AGG - Chr4:72600163-72600182 MsG0480022444.01.T01:intron 50.0%
AATCCCGCGATGAATTTGCC+TGG - Chr4:72599977-72599996 MsG0480022444.01.T01:three_prime_UTR 50.0%
AATTCTGGCCTCCCATACAC+TGG + Chr4:72600135-72600154 None:intergenic 50.0%
ACACGTGTGGGTATGAGTAC+AGG + Chr4:72599586-72599605 None:intergenic 50.0%
ACCAACCTGTTTCAGCAGCA+AGG + Chr4:72601322-72601341 None:intergenic 50.0%
ACCGCCTCTTTATTCCCAAC+CGG + Chr4:72599744-72599763 None:intergenic 50.0%
ACCGGTTGGGAATAAAGAGG+CGG - Chr4:72599740-72599759 MsG0480022444.01.T01:three_prime_UTR 50.0%
AGAGCCAGGCAAATTCATCG+CGG + Chr4:72599984-72600003 None:intergenic 50.0%
AGGATAACTGATGCTGCAGC+TGG + Chr4:72601158-72601177 None:intergenic 50.0%
CAATCTTCCCATCTGGACGA+AGG + Chr4:72600054-72600073 None:intergenic 50.0%
CAGTACTTTGTGCTGTGGAC+AGG - Chr4:72601570-72601589 MsG0480022444.01.T01:CDS 50.0%
CATGTCCTTGCTGCTGAAAC+AGG - Chr4:72601314-72601333 MsG0480022444.01.T01:CDS 50.0%
CCAACCTGATCATCTTCAGC+AGG + Chr4:72599672-72599691 None:intergenic 50.0%
CCTGCTGAAGATGATCAGGT+TGG - Chr4:72599669-72599688 MsG0480022444.01.T01:three_prime_UTR 50.0%
CTCAAGGATCTTCGTGGTTG+TGG - Chr4:72600160-72600179 MsG0480022444.01.T01:intron 50.0%
GAAGTCCAGTAAGAAGCGAC+CGG - Chr4:72599722-72599741 MsG0480022444.01.T01:three_prime_UTR 50.0%
GATCCTTGAGCCCCAAATTC+TGG + Chr4:72600150-72600169 None:intergenic 50.0%
GCATATTGCCTTCGTCCAGA+TGG - Chr4:72600043-72600062 MsG0480022444.01.T01:CDS 50.0%
GCTGCAGCATCAGTTATCCT+TGG - Chr4:72601158-72601177 MsG0480022444.01.T01:intron 50.0%
GGGAGGATTGAAGGGGAAAT+TGG - Chr4:72599923-72599942 MsG0480022444.01.T01:three_prime_UTR 50.0%
GTATGGGAGGCCAGAATTTG+GGG - Chr4:72600137-72600156 MsG0480022444.01.T01:intron 50.0%
GTGTATGGGAGGCCAGAATT+TGG - Chr4:72600135-72600154 MsG0480022444.01.T01:intron 50.0%
TCAGCAGCAATCTTCCCATC+TGG + Chr4:72600061-72600080 None:intergenic 50.0%
TCCTTGCTGCTGAAACAGGT+TGG - Chr4:72601318-72601337 MsG0480022444.01.T01:CDS 50.0%
TCGACCTTTCGTTGTCTCCA+TGG - Chr4:72601106-72601125 MsG0480022444.01.T01:intron 50.0%
TGGGAAGATTGCTGCTGAAG+TGG - Chr4:72600063-72600082 MsG0480022444.01.T01:intron 50.0%
TGTCCCTGCTGAAGATGATC+AGG - Chr4:72599665-72599684 MsG0480022444.01.T01:three_prime_UTR 50.0%
! AGCAAGGACATGCTGACTAC+AGG + Chr4:72601306-72601325 None:intergenic 50.0%
! CCGAATGAGGATGGATTCGA+TGG - Chr4:72600091-72600110 MsG0480022444.01.T01:intron 50.0%
ATCGCGGGATTCACATTCGC+GGG + Chr4:72599968-72599987 None:intergenic 55.0%
CATCGCGGGATTCACATTCG+CGG + Chr4:72599969-72599988 None:intergenic 55.0%
CCAGTAAGAAGCGACCGGTT+GGG - Chr4:72599727-72599746 MsG0480022444.01.T01:three_prime_UTR 55.0%
GAGAGTCTCCAGTGTATGGG+AGG - Chr4:72600124-72600143 MsG0480022444.01.T01:intron 55.0%
GAGCCAGGCAAATTCATCGC+GGG + Chr4:72599983-72600002 None:intergenic 55.0%
GATCTTCGTGGTTGTGGAGG+TGG - Chr4:72600166-72600185 MsG0480022444.01.T01:intron 55.0%
GATGAGGAAGTTGCAAGGGC+AGG - Chr4:72599848-72599867 MsG0480022444.01.T01:three_prime_UTR 55.0%
GCGACCGGTTGGGAATAAAG+AGG - Chr4:72599737-72599756 MsG0480022444.01.T01:three_prime_UTR 55.0%
GGGTTCATCACATGAGAGCC+AGG + Chr4:72599998-72600017 None:intergenic 55.0%
TCCAGTAAGAAGCGACCGGT+TGG - Chr4:72599726-72599745 MsG0480022444.01.T01:three_prime_UTR 55.0%
TGCGCAGCAGAGGGTCAAAA+AGG + Chr4:72601207-72601226 None:intergenic 55.0%
TTGGGGCTCAAGGATCTTCG+TGG - Chr4:72600154-72600173 MsG0480022444.01.T01:intron 55.0%
! AGGCCAGAATTTGGGGCTCA+AGG - Chr4:72600144-72600163 MsG0480022444.01.T01:intron 55.0%
!! ACCAGGTGGGTTACACGTGT+GGG + Chr4:72599598-72599617 None:intergenic 55.0%
ACCCACACGTGTAACCCACC+TGG - Chr4:72599594-72599613 MsG0480022444.01.T01:three_prime_UTR 60.0%
CCCAACCGGTCGCTTCTTAC+TGG + Chr4:72599730-72599749 None:intergenic 60.0%
GAAAGCTAGGGCTGCGTCGT+TGG - Chr4:72599785-72599804 MsG0480022444.01.T01:three_prime_UTR 60.0%
GACCCTCTGCTGCGCAATGA+TGG - Chr4:72601211-72601230 MsG0480022444.01.T01:CDS 60.0%
GTTGCAAGGGCAGGCTGCTT+TGG - Chr4:72599857-72599876 MsG0480022444.01.T01:three_prime_UTR 60.0%
TGAAGTGGCGTCGCCGAATG+AGG - Chr4:72600078-72600097 MsG0480022444.01.T01:intron 60.0%
! CTTTGGAGGCCGAAGTTGCG+AGG - Chr4:72599874-72599893 MsG0480022444.01.T01:three_prime_UTR 60.0%
!! GACCAGGTGGGTTACACGTG+TGG + Chr4:72599599-72599618 None:intergenic 60.0%
AGCTAGGGCTGCGTCGTTGG+AGG - Chr4:72599788-72599807 MsG0480022444.01.T01:three_prime_UTR 65.0%
GGCTGCGTCGTTGGAGGATG+AGG - Chr4:72599794-72599813 MsG0480022444.01.T01:three_prime_UTR 65.0%
GGGTCTCGTGCAGCCAAAGC+CGG - Chr4:72601061-72601080 MsG0480022444.01.T01:intron 65.0%
GTGGCGTCGCCGAATGAGGA+TGG - Chr4:72600082-72600101 MsG0480022444.01.T01:intron 65.0%
! GCAAGGGCAGGCTGCTTTGG+AGG - Chr4:72599860-72599879 MsG0480022444.01.T01:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 72599462 72601659 72599462 ID=MsG0480022444.01;Name=MsG0480022444.01
Chr4 mRNA 72599462 72601659 72599462 ID=MsG0480022444.01.T01;Parent=MsG0480022444.01;Name=MsG0480022444.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0.33|1|0|0|3|572|227
Chr4 exon 72599462 72600062 72599462 ID=MsG0480022444.01.T01:exon:5280;Parent=MsG0480022444.01.T01
Chr4 exon 72600898 72601059 72600898 ID=MsG0480022444.01.T01:exon:5279;Parent=MsG0480022444.01.T01
Chr4 exon 72601167 72601659 72601167 ID=MsG0480022444.01.T01:exon:5278;Parent=MsG0480022444.01.T01
Chr4 CDS 72601167 72601659 72601167 ID=MsG0480022444.01.T01:cds;Parent=MsG0480022444.01.T01
Chr4 CDS 72600898 72601059 72600898 ID=MsG0480022444.01.T01:cds;Parent=MsG0480022444.01.T01
Chr4 CDS 72600034 72600062 72600034 ID=MsG0480022444.01.T01:cds;Parent=MsG0480022444.01.T01
Chr4 three_prime_UTR 72599462 72600033 72599462 ID=MsG0480022444.01.T01:three_prime_utr;Parent=MsG0480022444.01.T01
Gene Sequence

>MsG0480022444.01.T01

ATGGAAGACGGTGAACTTGATTTCTCAAATCAAGTCGTGTTTTCGAGTCCAAACATGGCTGAATTTCAAACAAGTGGTTCAATGGATAGTTTTTTTGATGAATTGTTGAAGGATACACACGCCTGTACTCATACCCACACGTGTAACCCACCTGGTCCTGATTTTTCACACACTCACACGTGTTATCATGTTCACACCAAGATTGTCCCTGCTGAAGATGATCAGGTTGGGACTGATGATACTGCTGAATCTGCTGAGAAGAAGTCCAGTAAGAAGCGACCGGTTGGGAATAAAGAGGCGGTTAAGAAGTATCGTGAGAAGAAGAAAGCTAGGGCTGCGTCGTTGGAGGATGAGGTCATGAAATTGAGGGCTTTGAATCAGCATTTGATGAGGAAGTTGCAAGGGCAGGCTGCTTTGGAGGCCGAAGTTGCGAGGTTGAAGTGTTTGTTGGTTGATATTAGGGGAGGATTGAAGGGGAAATTGGGTCGTTTCCATGGGAAGATTGCTGCTGAAGTGGCGTCGCCGAATGAGGATGGATTCGATGGTTGTGAGTTTGAGAGTCTCCAGTGTATGGGAGGCCAGAATTTGGGGCTCAAGGATCTTCGTGGTTGTGGAGGTGGACCGACGTTGTCTAATGTCAATTCTCAAGCTTCCAGGGGGTCTCGTGCAGCCAAAGCCGGATGA
Protein sequence

>MsG0480022444.01.T01

MEDGELDFSNQVVFSSPNMAEFQTSGSMDSFFDELLKDTHACTHTHTCNPPGPDFSHTHTCYHVHTKIVPAEDDQVGTDDTAESAEKKSSKKRPVGNKEAVKKYREKKKARAASLEDEVMKLRALNQHLMRKLQGQAALEAEVARLKCLLVDIRGGLKGKLGRFHGKIAAEVASPNEDGFDGCEFESLQCMGGQNLGLKDLRGCGGGPTLSNVNSQASRGSRAAKAG*