Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022670.01.T01 | XP_013457199.1 | 98.45 | 129 | 2 | 0 | 23 | 151 | 114 | 242 | 5.15E-88 | 267 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022670.01.T01 | I3SQK3 | 98.450 | 129 | 2 | 0 | 23 | 151 | 114 | 242 | 2.46e-88 | 267 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480022670.01 | MsG0480023009.01 | 0.802449 | 5.580516e-49 | 2.734380e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022670.01.T01 | MTR_4g094252 | 98.450 | 129 | 2 | 0 | 23 | 151 | 114 | 242 | 6.24e-92 | 267 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022670.01.T01 | AT5G64160 | 76.378 | 127 | 19 | 4 | 26 | 151 | 111 | 227 | 3.43e-63 | 193 |
Find 58 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCGTTGATCCTTTCTTCT+TGG | 0.202699 | 4:+75334241 | None:intergenic |
| TTCTTCTTGGCCTCTGCACT+TGG | 0.301542 | 4:+75334254 | None:intergenic |
| ATCGACAAATATTAATGAAA+TGG | 0.334136 | 4:+75334928 | None:intergenic |
| CAACACTGTCGCAAACTTGC+TGG | 0.341217 | 4:+75334077 | None:intergenic |
| TGTCGATGTGGGTGCATATC+TGG | 0.359773 | 4:-75334912 | MsG0480022670.01.T01:CDS |
| TCCAACTCGCATGAGATGAT+TGG | 0.381351 | 4:+75334186 | None:intergenic |
| CAGCATTTCGTAATCTTGTT+CGG | 0.404676 | 4:+75333933 | None:intergenic |
| TTGGCCTCTGCACTTGGATC+CGG | 0.420143 | 4:+75334260 | None:intergenic |
| CTCTGCAGCATCTTCAGCAT+TGG | 0.420774 | 4:+75334748 | None:intergenic |
| TCGACAAATATTAATGAAAT+GGG | 0.433798 | 4:+75334929 | None:intergenic |
| TCTGCACTTGGATCCGGCGG+AGG | 0.435867 | 4:+75334266 | None:intergenic |
| GAGGCAAAAGCTCAACTAGT+TGG | 0.439322 | 4:-75334875 | MsG0480022670.01.T01:CDS |
| TCTGCAGCATCTTCAGCATT+GGG | 0.439771 | 4:+75334749 | None:intergenic |
| ATAATTTGCCATTGCTGCAT+CGG | 0.451444 | 4:+75334956 | None:intergenic |
| AGCATCTTCAGCATTGGGAT+TGG | 0.451701 | 4:+75334754 | None:intergenic |
| TTCATTAATATTTGTCGATG+TGG | 0.459022 | 4:-75334924 | MsG0480022670.01.T01:CDS |
| GCATTGGGATTGGTTGCAGT+TGG | 0.459137 | 4:+75334764 | None:intergenic |
| ACGTGCACTGGAGTAGGTCT+TGG | 0.462600 | 4:+75334319 | None:intergenic |
| TCATTAATATTTGTCGATGT+GGG | 0.470103 | 4:-75334923 | MsG0480022670.01.T01:CDS |
| ACCGTGAGCACATCTGAAAC+AGG | 0.470137 | 4:-75334797 | MsG0480022670.01.T01:CDS |
| AGGCAAAAGCTCAACTAGTT+GGG | 0.477644 | 4:-75334874 | MsG0480022670.01.T01:CDS |
| GCAGACCATTCCACGTGCAC+TGG | 0.484635 | 4:+75334307 | None:intergenic |
| CGTGCACTGGAGTAGGTCTT+GGG | 0.486772 | 4:+75334320 | None:intergenic |
| GGAGGGCGATCACCACATAC+AGG | 0.489871 | 4:+75334284 | None:intergenic |
| TCAGATGTGCTCACGGTAGC+TGG | 0.492603 | 4:+75334803 | None:intergenic |
| AACACTGTCGCAAACTTGCT+GGG | 0.495066 | 4:+75334078 | None:intergenic |
| GCCTCTGCACTTGGATCCGG+CGG | 0.501837 | 4:+75334263 | None:intergenic |
| AGTGCAGAGGCCAAGAAGAA+AGG | 0.513841 | 4:-75334251 | MsG0480022670.01.T01:CDS |
| ATGTGGTGATCGCCCTCCGC+CGG | 0.519780 | 4:-75334279 | MsG0480022670.01.T01:CDS |
| CACATCTGAAACAGGCAAGC+GGG | 0.519902 | 4:-75334789 | MsG0480022670.01.T01:CDS |
| CTGCACTTGGATCCGGCGGA+GGG | 0.529093 | 4:+75334267 | None:intergenic |
| CTACTCCAGTGCACGTGGAA+TGG | 0.536334 | 4:-75334312 | MsG0480022670.01.T01:CDS |
| ATTATCTGTGTTTCCTACGT+AGG | 0.541405 | 4:-75334121 | MsG0480022670.01.T01:three_prime_UTR |
| GGTTAAATCCGATGCAGCAA+TGG | 0.542018 | 4:-75334964 | None:intergenic |
| GCCTGTTTCAGATGTGCTCA+CGG | 0.542793 | 4:+75334796 | None:intergenic |
| GCCAATCATCTCATGCGAGT+TGG | 0.547339 | 4:-75334187 | MsG0480022670.01.T01:CDS |
| CATTCATGCAATTGATGCAA+CGG | 0.548160 | 4:-75333876 | MsG0480022670.01.T01:three_prime_UTR |
| CGGTAGCTGGTGTTGGTGGT+TGG | 0.548234 | 4:+75334816 | None:intergenic |
| GTGCTCACGGTAGCTGGTGT+TGG | 0.563568 | 4:+75334809 | None:intergenic |
| CGAGTAAGAAGTGACATCAA+TGG | 0.565304 | 4:-75334842 | MsG0480022670.01.T01:CDS |
| TCTGGCAGGTGCGGTAGTAG+AGG | 0.582011 | 4:-75334894 | MsG0480022670.01.T01:CDS |
| CTCACGGTAGCTGGTGTTGG+TGG | 0.586032 | 4:+75334812 | None:intergenic |
| AAGATTACGAAATGCTGTGA+TGG | 0.589182 | 4:-75333927 | MsG0480022670.01.T01:three_prime_UTR |
| CATTCCACGTGCACTGGAGT+AGG | 0.589238 | 4:+75334313 | None:intergenic |
| AACAACACAGACTGATCAGA+GGG | 0.592301 | 4:+75333849 | None:intergenic |
| GTGAAACAGAGATGCAGCTG+AGG | 0.607972 | 4:-75334160 | MsG0480022670.01.T01:CDS |
| GATGTGGGTGCATATCTGGC+AGG | 0.609113 | 4:-75334908 | MsG0480022670.01.T01:CDS |
| GGAATGGTCTGCCCTGTATG+TGG | 0.616539 | 4:-75334296 | MsG0480022670.01.T01:CDS |
| GAGGGCGATCACCACATACA+GGG | 0.629392 | 4:+75334285 | None:intergenic |
| AAGACCTACTCCAGTGCACG+TGG | 0.634183 | 4:-75334317 | MsG0480022670.01.T01:CDS |
| TCCGCCGGATCCAAGTGCAG+AGG | 0.638891 | 4:-75334264 | MsG0480022670.01.T01:CDS |
| AAACAACACAGACTGATCAG+AGG | 0.646025 | 4:+75333848 | None:intergenic |
| GCACATCTGAAACAGGCAAG+CGG | 0.661954 | 4:-75334790 | MsG0480022670.01.T01:CDS |
| AGGATCAACGATCAAAGACA+AGG | 0.663899 | 4:-75334231 | MsG0480022670.01.T01:CDS |
| GTAAGAAGTGACATCAATGG+AGG | 0.689619 | 4:-75334839 | MsG0480022670.01.T01:CDS |
| GGGTGCATATCTGGCAGGTG+CGG | 0.696245 | 4:-75334903 | MsG0480022670.01.T01:CDS |
| TATAGAATGTGTGTGCAGTG+TGG | 0.715016 | 4:-75334347 | MsG0480022670.01.T01:intron |
| TCACAGCATGAAACCTACGT+AGG | 0.733210 | 4:+75334108 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATAAAAATAATATTTTC+AGG | + | Chr4:75334124-75334143 | None:intergenic | 10.0% |
| !! | ATCGACAAATATTAATGAAA+TGG | + | Chr4:75333878-75333897 | None:intergenic | 20.0% |
| !! | TCGACAAATATTAATGAAAT+GGG | + | Chr4:75333877-75333896 | None:intergenic | 20.0% |
| !!! | TTTTTCCTTTAAAGCAAAAT+TGG | - | Chr4:75334342-75334361 | MsG0480022670.01.T01:CDS | 20.0% |
| ! | AAGACAAGGAAAAATCTAAA+AGG | - | Chr4:75334586-75334605 | MsG0480022670.01.T01:intron | 25.0% |
| ! | TCATTAATATTTGTCGATGT+GGG | - | Chr4:75333880-75333899 | MsG0480022670.01.T01:three_prime_UTR | 25.0% |
| ! | TTCATTAATATTTGTCGATG+TGG | - | Chr4:75333879-75333898 | MsG0480022670.01.T01:three_prime_UTR | 25.0% |
| !! | TGGGTAGATTTTATGTTTTT+TGG | - | Chr4:75334398-75334417 | MsG0480022670.01.T01:intron | 25.0% |
| AGGAAAAATCTAAAAGGATG+AGG | - | Chr4:75334592-75334611 | MsG0480022670.01.T01:intron | 30.0% | |
| GAAAAATCTAAAAGGATGAG+GGG | - | Chr4:75334594-75334613 | MsG0480022670.01.T01:intron | 30.0% | |
| GGAAAAATCTAAAAGGATGA+GGG | - | Chr4:75334593-75334612 | MsG0480022670.01.T01:intron | 30.0% | |
| GTATGTATTATTCCTTTCTG+AGG | - | Chr4:75334086-75334105 | MsG0480022670.01.T01:three_prime_UTR | 30.0% | |
| ! | ACATGCCAATTTTGCTTTAA+AGG | + | Chr4:75334350-75334369 | None:intergenic | 30.0% |
| AAAGCAAAATTGGCATGTAG+AGG | - | Chr4:75334352-75334371 | MsG0480022670.01.T01:intron | 35.0% | |
| AAGATTACGAAATGCTGTGA+TGG | - | Chr4:75334876-75334895 | MsG0480022670.01.T01:CDS | 35.0% | |
| ACATACTGTTTCATGTGTGT+GGG | - | Chr4:75334379-75334398 | MsG0480022670.01.T01:intron | 35.0% | |
| ATTATCTGTGTTTCCTACGT+AGG | - | Chr4:75334682-75334701 | MsG0480022670.01.T01:intron | 35.0% | |
| CAGCATTTCGTAATCTTGTT+CGG | + | Chr4:75334873-75334892 | None:intergenic | 35.0% | |
| CATTCATGCAATTGATGCAA+CGG | - | Chr4:75334927-75334946 | MsG0480022670.01.T01:CDS | 35.0% | |
| AGGATCAACGATCAAAGACA+AGG | - | Chr4:75334572-75334591 | MsG0480022670.01.T01:intron | 40.0% | |
| AGGCAAAAGCTCAACTAGTT+GGG | - | Chr4:75333929-75333948 | MsG0480022670.01.T01:three_prime_UTR | 40.0% | |
| CACATACTGTTTCATGTGTG+TGG | - | Chr4:75334378-75334397 | MsG0480022670.01.T01:intron | 40.0% | |
| CAGACTACATGCAGTATACT+TGG | + | Chr4:75334159-75334178 | None:intergenic | 40.0% | |
| CGAGTAAGAAGTGACATCAA+TGG | - | Chr4:75333961-75333980 | MsG0480022670.01.T01:three_prime_UTR | 40.0% | |
| GATCGTTGATCCTTTCTTCT+TGG | + | Chr4:75334565-75334584 | None:intergenic | 40.0% | |
| GCTAATACATGAACACCTCT+TGG | + | Chr4:75334222-75334241 | None:intergenic | 40.0% | |
| GTAAGAAGTGACATCAATGG+AGG | - | Chr4:75333964-75333983 | MsG0480022670.01.T01:three_prime_UTR | 40.0% | |
| TATAGAATGTGTGTGCAGTG+TGG | - | Chr4:75334456-75334475 | MsG0480022670.01.T01:intron | 40.0% | |
| TCCTAACTACTACCTCAGAA+AGG | + | Chr4:75334101-75334120 | None:intergenic | 40.0% | |
| !! | TCCTTTCTGAGGTAGTAGTT+AGG | - | Chr4:75334097-75334116 | MsG0480022670.01.T01:three_prime_UTR | 40.0% |
| CACCTACTGCAAACTTGCAT+AGG | + | Chr4:75334193-75334212 | None:intergenic | 45.0% | |
| CAGTAGGTGTATCTTCCAAG+AGG | - | Chr4:75334204-75334223 | MsG0480022670.01.T01:CDS | 45.0% | |
| CTCCTATGCAAGTTTGCAGT+AGG | - | Chr4:75334188-75334207 | MsG0480022670.01.T01:CDS | 45.0% | |
| GAGGCAAAAGCTCAACTAGT+TGG | - | Chr4:75333928-75333947 | MsG0480022670.01.T01:three_prime_UTR | 45.0% | |
| TCACAGCATGAAACCTACGT+AGG | + | Chr4:75334698-75334717 | None:intergenic | 45.0% | |
| TCCAACTCGCATGAGATGAT+TGG | + | Chr4:75334620-75334639 | None:intergenic | 45.0% | |
| TCTGCAGCATCTTCAGCATT+GGG | + | Chr4:75334057-75334076 | None:intergenic | 45.0% | |
| ! | AACACTGTCGCAAACTTGCT+GGG | + | Chr4:75334728-75334747 | None:intergenic | 45.0% |
| ! | AACTTGCTCGCAGCATTATG+AGG | - | Chr4:75334285-75334304 | MsG0480022670.01.T01:CDS | 45.0% |
| ! | AAGATGCTGCAGAGCTTTTG+AGG | - | Chr4:75334064-75334083 | MsG0480022670.01.T01:three_prime_UTR | 45.0% |
| ! | AGCATCTTCAGCATTGGGAT+TGG | + | Chr4:75334052-75334071 | None:intergenic | 45.0% |
| AGTGCAGAGGCCAAGAAGAA+AGG | - | Chr4:75334552-75334571 | MsG0480022670.01.T01:intron | 50.0% | |
| CAACACTGTCGCAAACTTGC+TGG | + | Chr4:75334729-75334748 | None:intergenic | 50.0% | |
| CTCTGCAGCATCTTCAGCAT+TGG | + | Chr4:75334058-75334077 | None:intergenic | 50.0% | |
| GCCAATCATCTCATGCGAGT+TGG | - | Chr4:75334616-75334635 | MsG0480022670.01.T01:intron | 50.0% | |
| GCCTGTTTCAGATGTGCTCA+CGG | + | Chr4:75334010-75334029 | None:intergenic | 50.0% | |
| GTGAAACAGAGATGCAGCTG+AGG | - | Chr4:75334643-75334662 | MsG0480022670.01.T01:intron | 50.0% | |
| TGTCGATGTGGGTGCATATC+TGG | - | Chr4:75333891-75333910 | MsG0480022670.01.T01:three_prime_UTR | 50.0% | |
| TTCTTCTTGGCCTCTGCACT+TGG | + | Chr4:75334552-75334571 | None:intergenic | 50.0% | |
| ! | ACCGTGAGCACATCTGAAAC+AGG | - | Chr4:75334006-75334025 | MsG0480022670.01.T01:three_prime_UTR | 50.0% |
| ! | CACATCTGAAACAGGCAAGC+GGG | - | Chr4:75334014-75334033 | MsG0480022670.01.T01:three_prime_UTR | 50.0% |
| ! | GCACATCTGAAACAGGCAAG+CGG | - | Chr4:75334013-75334032 | MsG0480022670.01.T01:three_prime_UTR | 50.0% |
| !! | GCATTGGGATTGGTTGCAGT+TGG | + | Chr4:75334042-75334061 | None:intergenic | 50.0% |
| AAGACCTACTCCAGTGCACG+TGG | - | Chr4:75334486-75334505 | MsG0480022670.01.T01:intron | 55.0% | |
| ACGTGCACTGGAGTAGGTCT+TGG | + | Chr4:75334487-75334506 | None:intergenic | 55.0% | |
| CATTCCACGTGCACTGGAGT+AGG | + | Chr4:75334493-75334512 | None:intergenic | 55.0% | |
| CGTGCACTGGAGTAGGTCTT+GGG | + | Chr4:75334486-75334505 | None:intergenic | 55.0% | |
| CTACTCCAGTGCACGTGGAA+TGG | - | Chr4:75334491-75334510 | MsG0480022670.01.T01:intron | 55.0% | |
| GAGGGCGATCACCACATACA+GGG | + | Chr4:75334521-75334540 | None:intergenic | 55.0% | |
| GATGTGGGTGCATATCTGGC+AGG | - | Chr4:75333895-75333914 | MsG0480022670.01.T01:three_prime_UTR | 55.0% | |
| GGAATGGTCTGCCCTGTATG+TGG | - | Chr4:75334507-75334526 | MsG0480022670.01.T01:intron | 55.0% | |
| TCAGATGTGCTCACGGTAGC+TGG | + | Chr4:75334003-75334022 | None:intergenic | 55.0% | |
| TTGGCCTCTGCACTTGGATC+CGG | + | Chr4:75334546-75334565 | None:intergenic | 55.0% | |
| GCAGACCATTCCACGTGCAC+TGG | + | Chr4:75334499-75334518 | None:intergenic | 60.0% | |
| GGAGGGCGATCACCACATAC+AGG | + | Chr4:75334522-75334541 | None:intergenic | 60.0% | |
| GGGTGCATATCTGGCAGGTG+CGG | - | Chr4:75333900-75333919 | MsG0480022670.01.T01:three_prime_UTR | 60.0% | |
| GTGCTCACGGTAGCTGGTGT+TGG | + | Chr4:75333997-75334016 | None:intergenic | 60.0% | |
| TCTGGCAGGTGCGGTAGTAG+AGG | - | Chr4:75333909-75333928 | MsG0480022670.01.T01:three_prime_UTR | 60.0% | |
| ! | CTCACGGTAGCTGGTGTTGG+TGG | + | Chr4:75333994-75334013 | None:intergenic | 60.0% |
| !! | CGGTAGCTGGTGTTGGTGGT+TGG | + | Chr4:75333990-75334009 | None:intergenic | 60.0% |
| ATGTGGTGATCGCCCTCCGC+CGG | - | Chr4:75334524-75334543 | MsG0480022670.01.T01:intron | 65.0% | |
| CTGCACTTGGATCCGGCGGA+GGG | + | Chr4:75334539-75334558 | None:intergenic | 65.0% | |
| GCCTCTGCACTTGGATCCGG+CGG | + | Chr4:75334543-75334562 | None:intergenic | 65.0% | |
| TCCGCCGGATCCAAGTGCAG+AGG | - | Chr4:75334539-75334558 | MsG0480022670.01.T01:intron | 65.0% | |
| TCTGCACTTGGATCCGGCGG+AGG | + | Chr4:75334540-75334559 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 75333850 | 75334975 | 75333850 | ID=MsG0480022670.01;Name=MsG0480022670.01 |
| Chr4 | mRNA | 75333850 | 75334975 | 75333850 | ID=MsG0480022670.01.T01;Parent=MsG0480022670.01;Name=MsG0480022670.01.T01;_AED=0.41;_eAED=0.46;_QI=0|1|0.5|1|1|1|2|295|151 |
| Chr4 | exon | 75333850 | 75334364 | 75333850 | ID=MsG0480022670.01.T01:exon:3221;Parent=MsG0480022670.01.T01 |
| Chr4 | exon | 75334740 | 75334975 | 75334740 | ID=MsG0480022670.01.T01:exon:3220;Parent=MsG0480022670.01.T01 |
| Chr4 | CDS | 75334740 | 75334975 | 75334740 | ID=MsG0480022670.01.T01:cds;Parent=MsG0480022670.01.T01 |
| Chr4 | CDS | 75334145 | 75334364 | 75334145 | ID=MsG0480022670.01.T01:cds;Parent=MsG0480022670.01.T01 |
| Chr4 | three_prime_UTR | 75333850 | 75334144 | 75333850 | ID=MsG0480022670.01.T01:three_prime_utr;Parent=MsG0480022670.01.T01 |
| Gene Sequence |
| Protein sequence |
