AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023009.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023009.01.T01 MTR_8g083210 92.857 140 10 0 15 154 762 901 8.78e-85 266
MsG0480023009.01.T01 MTR_8g083210 92.857 140 10 0 15 154 769 908 1.06e-84 266
MsG0480023009.01.T01 MTR_3g084500 83.571 140 23 0 15 154 539 678 2.42e-77 243
MsG0480023009.01.T01 MTR_3g084500 83.571 140 23 0 15 154 589 728 5.04e-77 243
MsG0480023009.01.T01 MTR_3g084500 83.571 140 23 0 15 154 759 898 4.21e-76 243
MsG0480023009.01.T01 MTR_3g079330 34.783 138 86 3 19 154 236 371 8.99e-19 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023009.01.T01 AT1G31230 72.857 140 38 0 15 154 764 903 4.01e-67 219
MsG0480023009.01.T01 AT4G19710 72.143 140 39 0 15 154 769 908 2.48e-66 217
MsG0480023009.01.T01 AT5G21060 39.098 133 77 3 24 154 241 371 4.05e-23 94.0
MsG0480023009.01.T01 AT5G21060 39.098 133 77 3 24 154 243 373 4.09e-23 94.0
MsG0480023009.01.T01 AT5G21060 39.098 133 77 3 24 154 243 373 4.39e-23 94.0
MsG0480023009.01.T01 AT4G19710 58.824 68 28 0 15 82 769 836 1.70e-21 90.5

Find 52 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAATTACAATGTCAATTT+TGG 0.115631 4:-79647345 None:intergenic
GCAAACTCCGGGTCAAATTT+TGG 0.204913 4:+79647055 None:intergenic
TAGCTTGTTTATCTTGTATA+CGG 0.229519 4:-79646069 MsG0480023009.01.T01:three_prime_UTR
GGAGGGGTTGTTGCAGGATA+TGG 0.232776 4:+79645930 None:intergenic
CTGTCTCCATTAATCTTGTT+TGG 0.265081 4:-79646037 MsG0480023009.01.T01:three_prime_UTR
CGACTCCCAAACAAGATTAA+TGG 0.286022 4:+79646031 None:intergenic
GTTCTTACCCGTAGTGGTTC+TGG 0.302693 4:+79647200 None:intergenic
TTACGACTTGCCTCATATCT+AGG 0.335031 4:-79646206 MsG0480023009.01.T01:exon
CATCCATTTGCACAATTGTC+GGG 0.343333 4:-79646340 MsG0480023009.01.T01:CDS
TGTCTCCATTAATCTTGTTT+GGG 0.352484 4:-79646036 MsG0480023009.01.T01:three_prime_UTR
ATAATTCTCGCAAGGGAATC+TGG 0.369464 4:-79647266 MsG0480023009.01.T01:CDS
TGTTCCTCTCTTGAGTTGTT+TGG 0.396214 4:-79646134 MsG0480023009.01.T01:three_prime_UTR
ATAGTTCGCGGGCCAGGAGC+TGG 0.412636 4:-79646265 MsG0480023009.01.T01:CDS
TAATTTAGACTGTCTCGAAT+AGG 0.417800 4:-79645980 MsG0480023009.01.T01:three_prime_UTR
TCTTGTTTCTTTGCAAACTC+CGG 0.422683 4:+79647043 None:intergenic
CAAGAAGATGCTGACAATGC+TGG 0.432766 4:-79647025 MsG0480023009.01.T01:CDS
GTGACTTGAGCACCAGCTCC+TGG 0.443485 4:+79646253 None:intergenic
AAGAAGATGCTGACAATGCT+GGG 0.463024 4:-79647024 MsG0480023009.01.T01:CDS
TCTGGCACTAGACTTTCAAT+TGG 0.463231 4:+79647218 None:intergenic
CCTCTCTTGAGTTGTTTGGT+AGG 0.469186 4:-79646130 MsG0480023009.01.T01:three_prime_UTR
CTTGTTTCTTTGCAAACTCC+GGG 0.470800 4:+79647044 None:intergenic
TCATCCATTTGCACAATTGT+CGG 0.476880 4:-79646341 MsG0480023009.01.T01:CDS
GACCCCGACAATTGTGCAAA+TGG 0.481521 4:+79646337 None:intergenic
CAAACAAGATTAATGGAGAC+AGG 0.481712 4:+79646038 None:intergenic
CCTCTGATAGTTCGCGGGCC+AGG 0.482336 4:-79646271 MsG0480023009.01.T01:CDS
ACAAAGCTCTGTTGTTGTAC+AGG 0.489559 4:-79647291 MsG0480023009.01.T01:CDS
AGCTTGTTTATCTTGTATAC+GGG 0.500385 4:-79646068 MsG0480023009.01.T01:three_prime_UTR
TCAGGCGTGTGCATCAGCTC+AGG 0.537437 4:-79647095 MsG0480023009.01.T01:intron
TGATGGAGCGCCTAGATATG+AGG 0.553770 4:+79646196 None:intergenic
AAATTGGTTCTTACCCGTAG+TGG 0.559667 4:+79647194 None:intergenic
GTCTAGTGCCAGAACCACTA+CGG 0.560015 4:-79647208 MsG0480023009.01.T01:CDS
GCTGGTGCTCAAGTCACAGC+GGG 0.561102 4:-79646247 MsG0480023009.01.T01:CDS
TTGCAGAATGACATCTATGA+TGG 0.572950 4:+79646179 None:intergenic
TCTAGTGCCAGAACCACTAC+GGG 0.573079 4:-79647207 MsG0480023009.01.T01:intron
AATCAACCTCTGATAGTTCG+CGG 0.577271 4:-79646277 MsG0480023009.01.T01:CDS
TACAGGTGATAATTCTCGCA+AGG 0.579848 4:-79647274 MsG0480023009.01.T01:CDS
ATCAACCTCTGATAGTTCGC+GGG 0.582260 4:-79646276 MsG0480023009.01.T01:CDS
GGTGCTCAAGTCACAGCGGG+TGG 0.586582 4:-79646244 MsG0480023009.01.T01:CDS
ATTGAGATACGTGGGAGTGG+TGG 0.596662 4:-79646413 MsG0480023009.01.T01:CDS
CCTGGCCCGCGAACTATCAG+AGG 0.604093 4:+79646271 None:intergenic
ATCCATTTGCACAATTGTCG+GGG 0.609087 4:-79646339 MsG0480023009.01.T01:CDS
GGTATTGAGATACGTGGGAG+TGG 0.611935 4:-79646416 MsG0480023009.01.T01:CDS
GGGTTGTTGCAGGATATGGA+TGG 0.623937 4:+79645934 None:intergenic
ACAGGTGATAATTCTCGCAA+GGG 0.634755 4:-79647273 MsG0480023009.01.T01:CDS
TAATTGCATTCACAACAAGA+AGG 0.639179 4:-79646306 MsG0480023009.01.T01:CDS
CCTACCAAACAACTCAAGAG+AGG 0.649702 4:+79646130 None:intergenic
AGAGCTGCGAAGATACAAGA+AGG 0.656222 4:-79646365 MsG0480023009.01.T01:CDS
TTGTAGGTATTGAGATACGT+GGG 0.666439 4:-79646421 MsG0480023009.01.T01:intron
TTTGTAGGTATTGAGATACG+TGG 0.669349 4:-79646422 MsG0480023009.01.T01:intron
ACAGCTACCAAAATTTGACC+CGG 0.679982 4:-79647062 MsG0480023009.01.T01:CDS
AGAAGATGCTGACAATGCTG+GGG 0.682028 4:-79647023 MsG0480023009.01.T01:CDS
AGCTGGTGCTCAAGTCACAG+CGG 0.710898 4:-79646248 MsG0480023009.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTATGAAATAAAGTTT+AGG + Chr4:79646560-79646579 None:intergenic 10.0%
!! AGAAAATAATGTATGTAATA+TGG + Chr4:79646410-79646429 None:intergenic 15.0%
!! ACTGCATAATTTATAGAAAT+TGG + Chr4:79646104-79646123 None:intergenic 20.0%
!! CAGATCTATTATAGAAATTT+AGG + Chr4:79646613-79646632 None:intergenic 20.0%
!! CCATCTTGAAAAAATAATTA+TGG + Chr4:79646754-79646773 None:intergenic 20.0%
!!! ATCGACATTACAAAATTTTA+TGG + Chr4:79647126-79647145 None:intergenic 20.0%
!!! CCATAATTATTTTTTCAAGA+TGG - Chr4:79646751-79646770 MsG0480023009.01.T01:intron 20.0%
! AAATAAAGTTTAGGTGAGTT+GGG + Chr4:79646551-79646570 None:intergenic 25.0%
! TCTATAAATTATGCAGTTCA+TGG - Chr4:79646107-79646126 MsG0480023009.01.T01:three_prime_UTR 25.0%
!! ATCTGAAGTAAAAGTGTAAA+AGG - Chr4:79646628-79646647 MsG0480023009.01.T01:intron 25.0%
!! CGAATAGGCATTTTAAATTT+TGG - Chr4:79647314-79647333 MsG0480023009.01.T01:CDS 25.0%
!!! CTACTTTTATTAAGTTTGAC+AGG + Chr4:79646485-79646504 None:intergenic 25.0%
!!! TAGCTTGTTTATCTTGTATA+CGG - Chr4:79647210-79647229 MsG0480023009.01.T01:CDS 25.0%
GAAATAAAGTTTAGGTGAGT+TGG + Chr4:79646552-79646571 None:intergenic 30.0%
GTGGATGTGACAAATAAAAA+AGG - Chr4:79646885-79646904 MsG0480023009.01.T01:intron 30.0%
TAATTGCATTCACAACAAGA+AGG - Chr4:79646973-79646992 MsG0480023009.01.T01:intron 30.0%
TAATTTAGACTGTCTCGAAT+AGG - Chr4:79647299-79647318 MsG0480023009.01.T01:CDS 30.0%
TACCTACAAAGTACAAACAT+AGG + Chr4:79646847-79646866 None:intergenic 30.0%
TCATGAACCAAAATTGACAT+AGG + Chr4:79645944-79645963 None:intergenic 30.0%
TTCCTATGTTTGTACTTTGT+AGG - Chr4:79646842-79646861 MsG0480023009.01.T01:intron 30.0%
! AGCTTGTTTATCTTGTATAC+GGG - Chr4:79647211-79647230 MsG0480023009.01.T01:CDS 30.0%
! TGTCTCCATTAATCTTGTTT+GGG - Chr4:79647243-79647262 MsG0480023009.01.T01:CDS 30.0%
!!! CTGTTTTGATGATTTCATTC+AGG - Chr4:79646166-79646185 MsG0480023009.01.T01:three_prime_UTR 30.0%
CAAACAAGATTAATGGAGAC+AGG + Chr4:79647244-79647263 None:intergenic 35.0%
CTGTCTCCATTAATCTTGTT+TGG - Chr4:79647242-79647261 MsG0480023009.01.T01:CDS 35.0%
TCATCCATTTGCACAATTGT+CGG - Chr4:79646938-79646957 MsG0480023009.01.T01:intron 35.0%
TTGCAGAATGACATCTATGA+TGG + Chr4:79647103-79647122 None:intergenic 35.0%
! AATAAAAGTAGAGTGCTCAC+AGG - Chr4:79646494-79646513 MsG0480023009.01.T01:intron 35.0%
! GGAAGCAAATTTAGAGTGTT+TGG - Chr4:79646524-79646543 MsG0480023009.01.T01:intron 35.0%
! TCTTGTTTCTTTGCAAACTC+CGG + Chr4:79646239-79646258 None:intergenic 35.0%
!! ACACTTTTTACTTCAGCTTG+TGG + Chr4:79646658-79646677 None:intergenic 35.0%
!! TTGTAGGTATTGAGATACGT+GGG - Chr4:79646858-79646877 MsG0480023009.01.T01:intron 35.0%
!! TTTGTAGGTATTGAGATACG+TGG - Chr4:79646857-79646876 MsG0480023009.01.T01:intron 35.0%
!!! TCAATTTTGGTTCATGACTG+TGG - Chr4:79645947-79645966 MsG0480023009.01.T01:three_prime_UTR 35.0%
AAACAGCCATGTCAAGCAAT+TGG + Chr4:79646152-79646171 None:intergenic 40.0%
AAGAAGATGCTGACAATGCT+GGG - Chr4:79646255-79646274 MsG0480023009.01.T01:CDS 40.0%
AATCAACCTCTGATAGTTCG+CGG - Chr4:79647002-79647021 MsG0480023009.01.T01:intron 40.0%
ACAAAGCTCTGTTGTTGTAC+AGG - Chr4:79645988-79646007 MsG0480023009.01.T01:three_prime_UTR 40.0%
ACAGCTACCAAAATTTGACC+CGG - Chr4:79646217-79646236 MsG0480023009.01.T01:CDS 40.0%
ACAGGTGATAATTCTCGCAA+GGG - Chr4:79646006-79646025 MsG0480023009.01.T01:three_prime_UTR 40.0%
ATAATTCTCGCAAGGGAATC+TGG - Chr4:79646013-79646032 MsG0480023009.01.T01:three_prime_UTR 40.0%
ATCCATTTGCACAATTGTCG+GGG - Chr4:79646940-79646959 MsG0480023009.01.T01:intron 40.0%
CATCCATTTGCACAATTGTC+GGG - Chr4:79646939-79646958 MsG0480023009.01.T01:intron 40.0%
CGACTCCCAAACAAGATTAA+TGG + Chr4:79647251-79647270 None:intergenic 40.0%
GCCAATCCAACATAAGTCAA+CGG + Chr4:79646683-79646702 None:intergenic 40.0%
TACAGGTGATAATTCTCGCA+AGG - Chr4:79646005-79646024 MsG0480023009.01.T01:three_prime_UTR 40.0%
TCTGGCACTAGACTTTCAAT+TGG + Chr4:79646064-79646083 None:intergenic 40.0%
TTACGACTTGCCTCATATCT+AGG - Chr4:79647073-79647092 MsG0480023009.01.T01:CDS 40.0%
! AAATTGGTTCTTACCCGTAG+TGG + Chr4:79646088-79646107 None:intergenic 40.0%
! CTTGTTTCTTTGCAAACTCC+GGG + Chr4:79646238-79646257 None:intergenic 40.0%
! GTTGACTTATGTTGGATTGG+CGG - Chr4:79646682-79646701 MsG0480023009.01.T01:intron 40.0%
! TGTTCCTCTCTTGAGTTGTT+TGG - Chr4:79647145-79647164 MsG0480023009.01.T01:intron 40.0%
!! ACCGTTGACTTATGTTGGAT+TGG - Chr4:79646679-79646698 MsG0480023009.01.T01:intron 40.0%
!! CGTTTCAACCTTGCTTTTCT+TGG + Chr4:79646458-79646477 None:intergenic 40.0%
!! GTGTTACCGTTGACTTATGT+TGG - Chr4:79646674-79646693 MsG0480023009.01.T01:intron 40.0%
!! TTGACTTATGTTGGATTGGC+GGG - Chr4:79646683-79646702 MsG0480023009.01.T01:intron 40.0%
AGAAGATGCTGACAATGCTG+GGG - Chr4:79646256-79646275 MsG0480023009.01.T01:CDS 45.0%
AGAGCTGCGAAGATACAAGA+AGG - Chr4:79646914-79646933 MsG0480023009.01.T01:intron 45.0%
ATCAACCTCTGATAGTTCGC+GGG - Chr4:79647003-79647022 MsG0480023009.01.T01:intron 45.0%
CAAGAAGATGCTGACAATGC+TGG - Chr4:79646254-79646273 MsG0480023009.01.T01:CDS 45.0%
CCAGAACCAATTGCTTGACA+TGG - Chr4:79646143-79646162 MsG0480023009.01.T01:three_prime_UTR 45.0%
CCTACAAGCCAAGAAAAGCA+AGG - Chr4:79646447-79646466 MsG0480023009.01.T01:intron 45.0%
CCTACCAAACAACTCAAGAG+AGG + Chr4:79647152-79647171 None:intergenic 45.0%
! AAAGTAGAGTGCTCACAGGT+TGG - Chr4:79646498-79646517 MsG0480023009.01.T01:intron 45.0%
! CCATGTCAAGCAATTGGTTC+TGG + Chr4:79646146-79646165 None:intergenic 45.0%
! CCTCTCTTGAGTTGTTTGGT+AGG - Chr4:79647149-79647168 MsG0480023009.01.T01:intron 45.0%
! GCAAACTCCGGGTCAAATTT+TGG + Chr4:79646227-79646246 None:intergenic 45.0%
!!! CCTTGCTTTTCTTGGCTTGT+AGG + Chr4:79646450-79646469 None:intergenic 45.0%
ATTGAGATACGTGGGAGTGG+TGG - Chr4:79646866-79646885 MsG0480023009.01.T01:intron 50.0%
GACCCCGACAATTGTGCAAA+TGG + Chr4:79646945-79646964 None:intergenic 50.0%
GTCTAGTGCCAGAACCACTA+CGG - Chr4:79646071-79646090 MsG0480023009.01.T01:three_prime_UTR 50.0%
GTTCTTACCCGTAGTGGTTC+TGG + Chr4:79646082-79646101 None:intergenic 50.0%
TCTAGTGCCAGAACCACTAC+GGG - Chr4:79646072-79646091 MsG0480023009.01.T01:three_prime_UTR 50.0%
! GGTATTGAGATACGTGGGAG+TGG - Chr4:79646863-79646882 MsG0480023009.01.T01:intron 50.0%
! TGATGGAGCGCCTAGATATG+AGG + Chr4:79647086-79647105 None:intergenic 50.0%
!! AGAGTGCTCACAGGTTGGTA+GGG - Chr4:79646503-79646522 MsG0480023009.01.T01:intron 50.0%
!! TAGAGTGCTCACAGGTTGGT+AGG - Chr4:79646502-79646521 MsG0480023009.01.T01:intron 50.0%
AGCTGGTGCTCAAGTCACAG+CGG - Chr4:79647031-79647050 MsG0480023009.01.T01:CDS 55.0%
GCTGGTGCTCAAGTCACAGC+GGG - Chr4:79647032-79647051 MsG0480023009.01.T01:CDS 60.0%
GTGACTTGAGCACCAGCTCC+TGG + Chr4:79647029-79647048 None:intergenic 60.0%
TCAGGCGTGTGCATCAGCTC+AGG - Chr4:79646184-79646203 MsG0480023009.01.T01:three_prime_UTR 60.0%
ATAGTTCGCGGGCCAGGAGC+TGG - Chr4:79647014-79647033 MsG0480023009.01.T01:intron 65.0%
CCTCTGATAGTTCGCGGGCC+AGG - Chr4:79647008-79647027 MsG0480023009.01.T01:intron 65.0%
CCTGGCCCGCGAACTATCAG+AGG + Chr4:79647011-79647030 None:intergenic 65.0%
GGTGCTCAAGTCACAGCGGG+TGG - Chr4:79647035-79647054 MsG0480023009.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 79645944 79647357 79645944 ID=MsG0480023009.01;Name=MsG0480023009.01
Chr4 mRNA 79645944 79647357 79645944 ID=MsG0480023009.01.T01;Parent=MsG0480023009.01;Name=MsG0480023009.01.T01;_AED=0.47;_eAED=0.50;_QI=0|1|0.66|1|1|1|3|263|157
Chr4 exon 79645944 79646437 79645944 ID=MsG0480023009.01.T01:exon:6118;Parent=MsG0480023009.01.T01
Chr4 exon 79647021 79647113 79647021 ID=MsG0480023009.01.T01:exon:6117;Parent=MsG0480023009.01.T01
Chr4 exon 79647208 79647357 79647208 ID=MsG0480023009.01.T01:exon:6116;Parent=MsG0480023009.01.T01
Chr4 CDS 79647208 79647357 79647208 ID=MsG0480023009.01.T01:cds;Parent=MsG0480023009.01.T01
Chr4 CDS 79647021 79647113 79647021 ID=MsG0480023009.01.T01:cds;Parent=MsG0480023009.01.T01
Chr4 CDS 79646207 79646437 79646207 ID=MsG0480023009.01.T01:cds;Parent=MsG0480023009.01.T01
Chr4 three_prime_UTR 79645944 79646206 79645944 ID=MsG0480023009.01.T01:three_prime_utr;Parent=MsG0480023009.01.T01
Gene Sequence

>MsG0480023009.01.T01

ATGTCAATTTTGGTTCATGACTGTGGTTATTCAAAATCAACATCACAAAGCTCTGTTGTTGTACAGGTGATAATTCTCGCAAGGGAATCTGGTTTAAAGCTAGAGCTGTCTAATATTCCAATTGAAAGTCTAGTGCCAGAACCACTACGGGCGTGTGCATCAGCTCAGGAGTTTATGCAACAGCTACCAAAATTTGACCCGGAGTTTGCAAAGAAACAAGAAGATGCTGACAATGCTGGGGAAGTATTGAGATACGTGGGAGTGGTGGATGTGACAAATAAAAAAGGAGTTGTAGAGCTGCGAAGATACAAGAAGGATCATCCATTTGCACAATTGTCGGGGTCAGATAATATAATTGCATTCACAACAAGAAGGTATAAGAATCAACCTCTGATAGTTCGCGGGCCAGGAGCTGGTGCTCAAGTCACAGCGGGTGGAATATTTAGTGATATTTACGACTTGCCTCATATCTAG

Protein sequence

>MsG0480023009.01.T01

MSILVHDCGYSKSTSQSSVVVQVIILARESGLKLELSNIPIESLVPEPLRACASAQEFMQQLPKFDPEFAKKQEDADNAGEVLRYVGVVDVTNKKGVVELRRYKKDHPFAQLSGSDNIIAFTTRRYKNQPLIVRGPGAGAQVTAGGIFSDIYDLPHI*