Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022820.01.T01 | PNY02465.1 | 98.193 | 166 | 3 | 0 | 1 | 166 | 1 | 166 | 1.54E-117 | 339 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022820.01.T01 | Q6E2Z6 | 98.193 | 166 | 3 | 0 | 1 | 166 | 53 | 218 | 1.89E-119 | 338 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022820.01.T01 | A0A2K3NHE8 | 98.193 | 166 | 3 | 0 | 1 | 166 | 1 | 166 | 7.36e-118 | 339 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0480022820.01 | MsG0880047318.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022820.01.T01 | MTR_4g094720 | 98.795 | 166 | 2 | 0 | 1 | 166 | 53 | 218 | 3.83e-121 | 340 |
| MsG0480022820.01.T01 | MTR_7g105830 | 29.839 | 124 | 81 | 2 | 6 | 128 | 130 | 248 | 3.04e-12 | 63.2 |
| MsG0480022820.01.T01 | MTR_1g105090 | 29.839 | 124 | 81 | 2 | 6 | 128 | 132 | 250 | 5.85e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022820.01.T01 | AT1G48130 | 73.214 | 168 | 38 | 2 | 1 | 166 | 53 | 215 | 1.99e-84 | 248 |
| MsG0480022820.01.T01 | AT5G06290 | 30.894 | 123 | 79 | 2 | 7 | 128 | 139 | 256 | 1.02e-13 | 67.4 |
| MsG0480022820.01.T01 | AT3G11630 | 31.452 | 124 | 79 | 2 | 6 | 128 | 131 | 249 | 2.91e-13 | 65.9 |
Find 44 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCTTAATGATTTCTCTTT+TGG | 0.116285 | 4:+77097613 | None:intergenic |
| GATATTTGTACCTTCTTATC+TGG | 0.262678 | 4:+77097518 | None:intergenic |
| TCCCCTGGCTTCCAGTTAGC+AGG | 0.316622 | 4:+77097319 | None:intergenic |
| ATGTTCCTCCCAGTTGTTGC+AGG | 0.342538 | 4:+77097403 | None:intergenic |
| CCATTGGAATCTTTCTCATC+AGG | 0.347454 | 4:+77097575 | None:intergenic |
| TTTCTGTACCCTGCAACAAC+TGG | 0.357498 | 4:-77097412 | MsG0480022820.01.T01:CDS |
| CATATCCTTAGCTTGTTCAT+TGG | 0.360719 | 4:+77097270 | None:intergenic |
| AAATTGCAACCCCTGCTAAC+TGG | 0.361486 | 4:-77097330 | MsG0480022820.01.T01:CDS |
| TGCAGATCTTCCATCCAAAA+AGG | 0.376713 | 4:-77097227 | MsG0480022820.01.T01:CDS |
| GCTTGTTCATTGGTAACATC+AGG | 0.425824 | 4:+77097280 | None:intergenic |
| ACAATATGAAGAGCCCTTGA+TGG | 0.428726 | 4:+77097545 | None:intergenic |
| ACCCCTGCTAACTGGAAGCC+AGG | 0.430708 | 4:-77097322 | MsG0480022820.01.T01:CDS |
| CCCCTGGCTTCCAGTTAGCA+GGG | 0.440132 | 4:+77097320 | None:intergenic |
| AAACGAGGAGTTATGCTGTT+AGG | 0.461288 | 4:-77097865 | MsG0480022820.01.T01:CDS |
| CAATGGAAATTTACCATCAA+GGG | 0.465608 | 4:-77097558 | MsG0480022820.01.T01:CDS |
| GGATAGTTCACCTTTGCACC+TGG | 0.476765 | 4:+77097650 | None:intergenic |
| CCAATGGAAATTTACCATCA+AGG | 0.485151 | 4:-77097559 | MsG0480022820.01.T01:CDS |
| CCTTGATGGTAAATTTCCAT+TGG | 0.485846 | 4:+77097559 | None:intergenic |
| ATCAAGGGCTCTTCATATTG+TGG | 0.511782 | 4:-77097543 | MsG0480022820.01.T01:CDS |
| CTGCAGTCTTAAAACCTTGA+GGG | 0.516211 | 4:+77097245 | None:intergenic |
| ACATCAGGAGAAATGACAAC+AGG | 0.519996 | 4:+77097295 | None:intergenic |
| TCAAGGGCTCTTCATATTGT+GGG | 0.523725 | 4:-77097542 | MsG0480022820.01.T01:CDS |
| TGTTCCTCCCAGTTGTTGCA+GGG | 0.527210 | 4:+77097404 | None:intergenic |
| TTCTGTACCCTGCAACAACT+GGG | 0.528210 | 4:-77097411 | MsG0480022820.01.T01:CDS |
| ATCTAGAATCTCACAAGGAG+TGG | 0.546589 | 4:-77097828 | MsG0480022820.01.T01:CDS |
| CCCCTGCTAACTGGAAGCCA+GGG | 0.547614 | 4:-77097321 | MsG0480022820.01.T01:CDS |
| CCCTGGCTTCCAGTTAGCAG+GGG | 0.564511 | 4:+77097321 | None:intergenic |
| TGTTACCAATGAACAAGCTA+AGG | 0.572171 | 4:-77097275 | MsG0480022820.01.T01:CDS |
| TATTGTGGGACCAGATAAGA+AGG | 0.572882 | 4:-77097528 | MsG0480022820.01.T01:intron |
| GAAATGACAACAGGTTCCCC+TGG | 0.579339 | 4:+77097304 | None:intergenic |
| GTAAATGCAGCCAGGTGCAA+AGG | 0.582844 | 4:-77097660 | MsG0480022820.01.T01:intron |
| TGCAACAACTGGGAGGAACA+TGG | 0.585961 | 4:-77097401 | MsG0480022820.01.T01:CDS |
| CCTGATGAGAAAGATTCCAA+TGG | 0.589101 | 4:-77097575 | MsG0480022820.01.T01:CDS |
| TCTGCAGTCTTAAAACCTTG+AGG | 0.590174 | 4:+77097244 | None:intergenic |
| GCTAAGGATATGTTCCCTCA+AGG | 0.596047 | 4:-77097259 | MsG0480022820.01.T01:CDS |
| GCAGGCACATTATCAAACAT+TGG | 0.624118 | 4:+77097189 | None:intergenic |
| GAACATGGATGAAGTGCTGA+GGG | 0.626828 | 4:-77097386 | MsG0480022820.01.T01:CDS |
| AATCATTAAGCAACTCAACA+TGG | 0.634966 | 4:-77097603 | MsG0480022820.01.T01:CDS |
| GGAACATGGATGAAGTGCTG+AGG | 0.641851 | 4:-77097387 | MsG0480022820.01.T01:CDS |
| GCTTCTGAGTTTAACAAACG+AGG | 0.664933 | 4:-77097880 | MsG0480022820.01.T01:CDS |
| CCCTGCTAACTGGAAGCCAG+GGG | 0.697213 | 4:-77097320 | MsG0480022820.01.T01:CDS |
| CATGGATGAAGTGCTGAGGG+TGG | 0.707751 | 4:-77097383 | MsG0480022820.01.T01:CDS |
| TGATGATCTAGAATCTCACA+AGG | 0.709676 | 4:-77097833 | MsG0480022820.01.T01:CDS |
| TGTACCCTGCAACAACTGGG+AGG | 0.722121 | 4:-77097408 | MsG0480022820.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAAATAGGAAAACTTA+CGG | + | Chr4:77097313-77097332 | None:intergenic | 20.0% |
| !! | ACAAATTAAACCATCATTTA+TGG | + | Chr4:77097644-77097663 | None:intergenic | 20.0% |
| !! | GAAACATGCATAGAAAATTT+TGG | + | Chr4:77097352-77097371 | None:intergenic | 25.0% |
| !! | TAAGAAGGTACAAATATCTA+TGG | - | Chr4:77097580-77097599 | MsG0480022820.01.T01:CDS | 25.0% |
| !! | TGCTTAATGATTTCTCTTTT+GGG | + | Chr4:77097482-77097501 | None:intergenic | 25.0% |
| !! | TTGCTTAATGATTTCTCTTT+TGG | + | Chr4:77097483-77097502 | None:intergenic | 25.0% |
| !!! | CTTTTTTCTTCCATAAATGA+TGG | - | Chr4:77097631-77097650 | MsG0480022820.01.T01:CDS | 25.0% |
| AATCATTAAGCAACTCAACA+TGG | - | Chr4:77097490-77097509 | MsG0480022820.01.T01:intron | 30.0% | |
| ATGCATGTGGAAAAGAAAAT+AGG | + | Chr4:77097324-77097343 | None:intergenic | 30.0% | |
| CAATGGAAATTTACCATCAA+GGG | - | Chr4:77097535-77097554 | MsG0480022820.01.T01:CDS | 30.0% | |
| GATATTTGTACCTTCTTATC+TGG | + | Chr4:77097578-77097597 | None:intergenic | 30.0% | |
| !!! | CTTTTGGGATCACTAATTAT+TGG | + | Chr4:77097467-77097486 | None:intergenic | 30.0% |
| !!! | TTTTTTATGTAAATGCAGCC+AGG | - | Chr4:77097425-77097444 | MsG0480022820.01.T01:CDS | 30.0% |
| CCAATGGAAATTTACCATCA+AGG | - | Chr4:77097534-77097553 | MsG0480022820.01.T01:CDS | 35.0% | |
| TGTTACCAATGAACAAGCTA+AGG | - | Chr4:77097818-77097837 | MsG0480022820.01.T01:CDS | 35.0% | |
| ! | CCTTGATGGTAAATTTCCAT+TGG | + | Chr4:77097537-77097556 | None:intergenic | 35.0% |
| ! | CTAAGATACTCCTTTTTGGA+TGG | + | Chr4:77097879-77097898 | None:intergenic | 35.0% |
| ! | GAACCTAAGATACTCCTTTT+TGG | + | Chr4:77097883-77097902 | None:intergenic | 35.0% |
| ! | TGATGATCTAGAATCTCACA+AGG | - | Chr4:77097260-77097279 | MsG0480022820.01.T01:CDS | 35.0% |
| !! | AATTTTGGTATGCATGCATG+TGG | + | Chr4:77097337-77097356 | None:intergenic | 35.0% |
| !! | CATATCCTTAGCTTGTTCAT+TGG | + | Chr4:77097826-77097845 | None:intergenic | 35.0% |
| !! | CATCCAAAAAGGAGTATCTT+AGG | - | Chr4:77097877-77097896 | MsG0480022820.01.T01:CDS | 35.0% |
| AAACGAGGAGTTATGCTGTT+AGG | - | Chr4:77097228-77097247 | MsG0480022820.01.T01:CDS | 40.0% | |
| ACAATATGAAGAGCCCTTGA+TGG | + | Chr4:77097551-77097570 | None:intergenic | 40.0% | |
| ACATCAGGAGAAATGACAAC+AGG | + | Chr4:77097801-77097820 | None:intergenic | 40.0% | |
| ATCAAGGGCTCTTCATATTG+TGG | - | Chr4:77097550-77097569 | MsG0480022820.01.T01:CDS | 40.0% | |
| CCATTGGAATCTTTCTCATC+AGG | + | Chr4:77097521-77097540 | None:intergenic | 40.0% | |
| CCTGATGAGAAAGATTCCAA+TGG | - | Chr4:77097518-77097537 | MsG0480022820.01.T01:intron | 40.0% | |
| CTGCAGTCTTAAAACCTTGA+GGG | + | Chr4:77097851-77097870 | None:intergenic | 40.0% | |
| GCTTCTGAGTTTAACAAACG+AGG | - | Chr4:77097213-77097232 | MsG0480022820.01.T01:CDS | 40.0% | |
| TATTGTGGGACCAGATAAGA+AGG | - | Chr4:77097565-77097584 | MsG0480022820.01.T01:CDS | 40.0% | |
| TCAAGGGCTCTTCATATTGT+GGG | - | Chr4:77097551-77097570 | MsG0480022820.01.T01:CDS | 40.0% | |
| TCTGCAGTCTTAAAACCTTG+AGG | + | Chr4:77097852-77097871 | None:intergenic | 40.0% | |
| TGCAGATCTTCCATCCAAAA+AGG | - | Chr4:77097866-77097885 | MsG0480022820.01.T01:CDS | 40.0% | |
| ! | ATCTAGAATCTCACAAGGAG+TGG | - | Chr4:77097265-77097284 | MsG0480022820.01.T01:CDS | 40.0% |
| ! | GCTTGTTCATTGGTAACATC+AGG | + | Chr4:77097816-77097835 | None:intergenic | 40.0% |
| AAATTGCAACCCCTGCTAAC+TGG | - | Chr4:77097763-77097782 | MsG0480022820.01.T01:intron | 45.0% | |
| GCTAAGGATATGTTCCCTCA+AGG | - | Chr4:77097834-77097853 | MsG0480022820.01.T01:CDS | 45.0% | |
| TTCTGTACCCTGCAACAACT+GGG | - | Chr4:77097682-77097701 | MsG0480022820.01.T01:intron | 45.0% | |
| TTTCTGTACCCTGCAACAAC+TGG | - | Chr4:77097681-77097700 | MsG0480022820.01.T01:intron | 45.0% | |
| !! | GAACATGGATGAAGTGCTGA+GGG | - | Chr4:77097707-77097726 | MsG0480022820.01.T01:intron | 45.0% |
| !!! | ATTGATAATATTAATTAATT+AGG | - | Chr4:77097395-77097414 | MsG0480022820.01.T01:CDS | 5.0% |
| ATGTTCCTCCCAGTTGTTGC+AGG | + | Chr4:77097693-77097712 | None:intergenic | 50.0% | |
| GAAATGACAACAGGTTCCCC+TGG | + | Chr4:77097792-77097811 | None:intergenic | 50.0% | |
| GGATAGTTCACCTTTGCACC+TGG | + | Chr4:77097446-77097465 | None:intergenic | 50.0% | |
| GTAAATGCAGCCAGGTGCAA+AGG | - | Chr4:77097433-77097452 | MsG0480022820.01.T01:intron | 50.0% | |
| TGCAACAACTGGGAGGAACA+TGG | - | Chr4:77097692-77097711 | MsG0480022820.01.T01:intron | 50.0% | |
| TGTTCCTCCCAGTTGTTGCA+GGG | + | Chr4:77097692-77097711 | None:intergenic | 50.0% | |
| !! | GGAACATGGATGAAGTGCTG+AGG | - | Chr4:77097706-77097725 | MsG0480022820.01.T01:intron | 50.0% |
| TGTACCCTGCAACAACTGGG+AGG | - | Chr4:77097685-77097704 | MsG0480022820.01.T01:intron | 55.0% | |
| !! | CATGGATGAAGTGCTGAGGG+TGG | - | Chr4:77097710-77097729 | MsG0480022820.01.T01:intron | 55.0% |
| ACCCCTGCTAACTGGAAGCC+AGG | - | Chr4:77097771-77097790 | MsG0480022820.01.T01:intron | 60.0% | |
| CCCCTGCTAACTGGAAGCCA+GGG | - | Chr4:77097772-77097791 | MsG0480022820.01.T01:intron | 60.0% | |
| CCCCTGGCTTCCAGTTAGCA+GGG | + | Chr4:77097776-77097795 | None:intergenic | 60.0% | |
| CCCTGCTAACTGGAAGCCAG+GGG | - | Chr4:77097773-77097792 | MsG0480022820.01.T01:intron | 60.0% | |
| CCCTGGCTTCCAGTTAGCAG+GGG | + | Chr4:77097775-77097794 | None:intergenic | 60.0% | |
| TCCCCTGGCTTCCAGTTAGC+AGG | + | Chr4:77097777-77097796 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 77097201 | 77097914 | 77097201 | ID=MsG0480022820.01;Name=MsG0480022820.01 |
| Chr4 | mRNA | 77097201 | 77097914 | 77097201 | ID=MsG0480022820.01.T01;Parent=MsG0480022820.01;Name=MsG0480022820.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|0.66|3|0|166 |
| Chr4 | exon | 77097804 | 77097914 | 77097804 | ID=MsG0480022820.01.T01:exon:19945;Parent=MsG0480022820.01.T01 |
| Chr4 | exon | 77097529 | 77097672 | 77097529 | ID=MsG0480022820.01.T01:exon:19944;Parent=MsG0480022820.01.T01 |
| Chr4 | exon | 77097201 | 77097446 | 77097201 | ID=MsG0480022820.01.T01:exon:19943;Parent=MsG0480022820.01.T01 |
| Chr4 | CDS | 77097804 | 77097914 | 77097804 | ID=MsG0480022820.01.T01:cds;Parent=MsG0480022820.01.T01 |
| Chr4 | CDS | 77097529 | 77097672 | 77097529 | ID=MsG0480022820.01.T01:cds;Parent=MsG0480022820.01.T01 |
| Chr4 | CDS | 77097201 | 77097446 | 77097201 | ID=MsG0480022820.01.T01:cds;Parent=MsG0480022820.01.T01 |
| Gene Sequence |
| Protein sequence |
