AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022822.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022822.01.T01 MTR_4g094730 81.720 372 31 3 1 335 1 372 0.0 543
MsG0480022822.01.T01 MTR_4g094730 80.120 332 29 3 1 295 1 332 0.0 521
MsG0480022822.01.T01 MTR_3g072410 29.932 294 154 10 38 289 29 312 3.40e-25 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022822.01.T01 AT2G17120 37.535 357 170 8 7 326 1 341 4.76e-65 209
MsG0480022822.01.T01 AT1G21880 27.273 330 187 10 1 289 1 318 8.38e-25 104
MsG0480022822.01.T01 AT1G21880 27.673 318 179 10 10 286 8 315 1.60e-24 102
MsG0480022822.01.T01 AT1G77630 26.687 326 183 10 5 289 5 315 2.31e-19 89.0

Find 105 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTGATGTTCCCCTTCC+AGG 0.233486 4:+77110860 MsG0480022822.01.T01:CDS
GGTTCCGGTGATTCAGATTC+AGG 0.253482 4:+77111977 MsG0480022822.01.T01:CDS
TCTTCTATTTAGTGCTTGTT+TGG 0.281345 4:+77112203 MsG0480022822.01.T01:three_prime_UTR
ACATACTATGTTACAAATAT+TGG 0.287591 4:-77112297 None:intergenic
TTTCTGTTCTTTCTACTGTT+TGG 0.294314 4:-77110102 None:intergenic
ATCTAAAACCGATAAATAAT+TGG 0.306172 4:+77111691 MsG0480022822.01.T01:CDS
TGTTCCCTACGCTCATGTTT+TGG 0.333621 4:-77110133 None:intergenic
AGACACTTGAAGTTTGCTTC+TGG 0.342284 4:-77110227 None:intergenic
CAAACCGACCAATTATTTAT+CGG 0.342832 4:-77111699 None:intergenic
GAGGGTTAAGTTTGCGGGTT+TGG 0.357501 4:+77110591 MsG0480022822.01.T01:CDS
TGTTCCGTTACTACATTTGC+AGG 0.366257 4:-77110435 None:intergenic
CAATCTTGTACTTGGGTACA+TGG 0.382662 4:-77110540 None:intergenic
TTGGTCGGTTTGTCCGTCTA+TGG 0.383787 4:+77111710 MsG0480022822.01.T01:CDS
CAGGGTTGCTATTTCCTTCA+AGG 0.391811 4:-77110306 None:intergenic
CAATCACCACACACAAAGAT+AGG 0.403847 4:+77110076 MsG0480022822.01.T01:five_prime_UTR
ATTAACTTTGTAACTGTTGT+TGG 0.409815 4:-77110384 None:intergenic
TGTTAGCTCCAAGGAGGTCT+AGG 0.416629 4:-77110346 None:intergenic
ATCTTTGTGTGTGGTGATTG+AGG 0.428552 4:-77110073 None:intergenic
ATAGCGAGGGTTAAGTTTGC+GGG 0.428857 4:+77110586 MsG0480022822.01.T01:CDS
AGACAAATAAGATGAGAAAG+TGG 0.430495 4:-77112054 None:intergenic
ACAGTGGCACCTAGTGGTTC+CGG 0.432003 4:+77111962 MsG0480022822.01.T01:intron
TTGTGTGTGGTGATTGAGGT+TGG 0.433597 4:-77110069 None:intergenic
CAAGGTTGTTGCGTTTGTGC+TGG 0.440828 4:-77110288 None:intergenic
CTAGGAGCATGTTGAATGGC+TGG 0.448470 4:+77112005 MsG0480022822.01.T01:CDS
CACAAACGCAACAACCTTGA+AGG 0.449829 4:+77110292 MsG0480022822.01.T01:CDS
ACCCGGTACAATCTTGTACT+TGG 0.454063 4:-77110548 None:intergenic
TATGCACATATTGTTCCAAT+GGG 0.455588 4:+77110737 MsG0480022822.01.T01:CDS
TGTTGTTGGTGTTGCTTGGA+AGG 0.464563 4:-77110370 None:intergenic
TGCAAATGTAGTAACGGAAC+AGG 0.465594 4:+77110437 MsG0480022822.01.T01:CDS
GGAACCTTGATGACTTGGTT+GGG 0.472297 4:-77110407 None:intergenic
TAACTGTTGTTGGTGTTGCT+TGG 0.473100 4:-77110374 None:intergenic
CAAACAAGTCACATAGATGC+AGG 0.476305 4:-77109858 None:intergenic
TGTCCGTCTATGGAATGCTC+CGG 0.477070 4:+77111720 MsG0480022822.01.T01:CDS
TTGCAGTAGTAGCAAGTGTT+GGG 0.480370 4:+77110183 MsG0480022822.01.T01:CDS
AGAAGAAGAATAGTCTATGA+TGG 0.483481 4:-77109904 None:intergenic
TACAAGATTGTACCGGGTGA+TGG 0.483742 4:+77110553 MsG0480022822.01.T01:CDS
GTTGCATTTGGCACAAGGAA+TGG 0.486915 4:-77111585 None:intergenic
TTACTACATTTGCAGGGGAA+TGG 0.490191 4:-77110428 None:intergenic
TTGACTTTGACTTATAAAGT+TGG 0.491522 4:-77109937 None:intergenic
GCTCCGGGAGTGTGATGCTT+GGG 0.496795 4:+77111736 MsG0480022822.01.T01:CDS
ATTCAGATTCAGGGGCATCT+AGG 0.497537 4:+77111987 MsG0480022822.01.T01:CDS
TCTAGACACCATAACTCTCC+TGG 0.498178 4:-77109813 None:intergenic
AAGGAAATAGCAACCCTGTT+CGG 0.499124 4:+77110311 MsG0480022822.01.T01:CDS
GATTGTACCGGGTGATGGGC+TGG 0.502517 4:+77110558 MsG0480022822.01.T01:CDS
GTGTAGAAATATGTTGCATT+TGG 0.504690 4:-77111597 None:intergenic
TGATCCATCCAGGAGAGTTA+TGG 0.508455 4:+77109805 None:intergenic
AATAGCGAGGGTTAAGTTTG+CGG 0.512277 4:+77110585 MsG0480022822.01.T01:CDS
TTATGCACATATTGTTCCAA+TGG 0.513134 4:+77110736 MsG0480022822.01.T01:CDS
TGCGTTTGTGCTGGTGTAGT+CGG 0.516449 4:-77110279 None:intergenic
CCCGGTACAATCTTGTACTT+GGG 0.517809 4:-77110547 None:intergenic
GGAACAAGATGGTCACTGTA+TGG 0.521397 4:+77110150 MsG0480022822.01.T01:CDS
GTTCCGGTGATTCAGATTCA+GGG 0.521820 4:+77111978 MsG0480022822.01.T01:CDS
ACAAGATTGTACCGGGTGAT+GGG 0.522910 4:+77110554 MsG0480022822.01.T01:CDS
TGCTCCGGGAGTGTGATGCT+TGG 0.525368 4:+77111735 MsG0480022822.01.T01:CDS
TGAATTCACATTATGAGTTG+AGG 0.527925 4:-77110010 None:intergenic
AAATGTAAATGCGACTCTAC+TGG 0.530306 4:+77111636 MsG0480022822.01.T01:CDS
TAGGAGCATGTTGAATGGCT+GGG 0.530960 4:+77112006 MsG0480022822.01.T01:CDS
TGCCCCTGAATCTGAATCAC+CGG 0.533305 4:-77111981 None:intergenic
CTTGTACTTGGGTACATGGT+TGG 0.536258 4:-77110536 None:intergenic
GTCCGTCTATGGAATGCTCC+GGG 0.536673 4:+77111721 MsG0480022822.01.T01:CDS
TTGTAAGAAATTACCTGGAA+GGG 0.540901 4:-77110873 None:intergenic
GTTCCCAAGCATCACACTCC+CGG 0.541349 4:-77111739 None:intergenic
GTTGCAGTAGTAGCAAGTGT+TGG 0.544877 4:+77110182 MsG0480022822.01.T01:CDS
AAACACTTCCTAGACCTCCT+TGG 0.549330 4:+77110338 MsG0480022822.01.T01:CDS
TAATCCCAACCAAGTCATCA+AGG 0.555223 4:+77110403 MsG0480022822.01.T01:CDS
GGAATGGAACCTTGATGACT+TGG 0.557647 4:-77110412 None:intergenic
CTCCCGGAGCATTCCATAGA+CGG 0.560810 4:-77111723 None:intergenic
AATATGTTGCATTTGGCACA+AGG 0.563072 4:-77111590 None:intergenic
TGTAGCTGTGATAAGGTGGA+TGG 0.564627 4:+77110698 MsG0480022822.01.T01:intron
TTGGAAGGTTGTTAGCTCCA+AGG 0.565038 4:-77110355 None:intergenic
TGTAAGAAATTACCTGGAAG+GGG 0.571154 4:-77110872 None:intergenic
GCATCTAGGAGCATGTTGAA+TGG 0.571353 4:+77112001 MsG0480022822.01.T01:CDS
GTTGTAAGAAATTACCTGGA+AGG 0.576069 4:-77110874 None:intergenic
GTCTATGATGGTGATGAAAG+AGG 0.576139 4:-77109892 None:intergenic
AACACCAAAACATGAGCGTA+GGG 0.577240 4:+77110129 MsG0480022822.01.T01:exon
GAGTATCAAAAGTGATTCAT+CGG 0.580179 4:+77111557 MsG0480022822.01.T01:CDS
AAAACCGATAAATAATTGGT+CGG 0.580244 4:+77111695 MsG0480022822.01.T01:CDS
ACCCAAGTACAAGATTGTAC+CGG 0.581624 4:+77110546 MsG0480022822.01.T01:CDS
GTTCCGTTACTACATTTGCA+GGG 0.582825 4:-77110434 None:intergenic
CCCAAGTACAAGATTGTACC+GGG 0.582853 4:+77110547 MsG0480022822.01.T01:CDS
AACACTTGCTACTACTGCAA+CGG 0.583006 4:-77110180 None:intergenic
GAACACCAAAACATGAGCGT+AGG 0.583823 4:+77110128 MsG0480022822.01.T01:exon
TGGTTTCCTATCTTTGTGTG+TGG 0.590687 4:-77110082 None:intergenic
TGGAACCTTGATGACTTGGT+TGG 0.596529 4:-77110408 None:intergenic
GATTGAATACTACTCCCCAT+TGG 0.596842 4:-77110752 None:intergenic
TTCCCCTGCAAATGTAGTAA+CGG 0.598287 4:+77110431 MsG0480022822.01.T01:CDS
GACACCATAACTCTCCTGGA+TGG 0.602502 4:-77109809 None:intergenic
ACTACTCAGAACACTTGTGC+CGG 0.606361 4:+77111840 MsG0480022822.01.T01:CDS
TCTGAATCACCGGAACCACT+AGG 0.612532 4:-77111971 None:intergenic
AGTGTTCTGAGTAGTAAGAG+TGG 0.614784 4:-77111832 None:intergenic
TGTACGACTACACGAAGCTG+TGG 0.617673 4:-77111769 None:intergenic
TTCCGTTACTACATTTGCAG+GGG 0.619937 4:-77110433 None:intergenic
TTCCGGTGATTCAGATTCAG+GGG 0.622806 4:+77111979 MsG0480022822.01.T01:CDS
CGTACAGTTTGTGATTACGC+TGG 0.625881 4:+77111786 MsG0480022822.01.T01:CDS
CAACAACAACTTACAATGTG+AGG 0.626373 4:+77111662 MsG0480022822.01.T01:CDS
ATTGCATCCAGCCCATCACC+CGG 0.634313 4:-77110565 None:intergenic
GATGGAAAGCATTAGCAGTG+TGG 0.635354 4:-77109975 None:intergenic
GTAGCTGTGATAAGGTGGAT+GGG 0.638620 4:+77110699 MsG0480022822.01.T01:intron
AAACAAGTCACATAGATGCA+GGG 0.649414 4:-77109857 None:intergenic
ATGGGCTGGATGCAATAGCG+AGG 0.663816 4:+77110572 MsG0480022822.01.T01:CDS
TGGGCTGGATGCAATAGCGA+GGG 0.677894 4:+77110573 MsG0480022822.01.T01:CDS
CATGAGCGTAGGGAACAAGA+TGG 0.678380 4:+77110139 MsG0480022822.01.T01:exon
GAAGGTTGTTAGCTCCAAGG+AGG 0.706118 4:-77110352 None:intergenic
ATGCACATATTGTTCCAATG+GGG 0.719899 4:+77110738 MsG0480022822.01.T01:CDS
GTCGGCTAAACTACGACACG+TGG 0.738036 4:-77110261 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAGTTACAAATTATCTATT+TGG + Chr4:77111879-77111898 MsG0480022822.01.T01:intron 15.0%
!!! TTTTTCTTTTTTTCTTTCTA+TGG + Chr4:77112111-77112130 MsG0480022822.01.T01:three_prime_UTR 15.0%
!! ACATACTATGTTACAAATAT+TGG - Chr4:77112300-77112319 None:intergenic 20.0%
!! ATTGATTACAAATGAATGTA+TGG + Chr4:77111340-77111359 MsG0480022822.01.T01:intron 20.0%
!! GTATTACATTCTTTCAATTA+CGG + Chr4:77111049-77111068 MsG0480022822.01.T01:intron 20.0%
!! TAGCTATATTTATTTACCTT+AGG - Chr4:77111382-77111401 None:intergenic 20.0%
!! TCTAATTGAACTAAAACATA+GGG - Chr4:77111485-77111504 None:intergenic 20.0%
!! TTCTAATTGAACTAAAACAT+AGG - Chr4:77111486-77111505 None:intergenic 20.0%
!!! AAACAATTCTTTTTCTTTGT+TGG - Chr4:77111274-77111293 None:intergenic 20.0%
!!! AACAATTCTTTTTCTTTGTT+GGG - Chr4:77111273-77111292 None:intergenic 20.0%
!!! ATCTAAAACCGATAAATAAT+TGG + Chr4:77111691-77111710 MsG0480022822.01.T01:CDS 20.0%
! AAAACCGATAAATAATTGGT+CGG + Chr4:77111695-77111714 MsG0480022822.01.T01:CDS 25.0%
! AAGATAACTAAAAGTCTGTT+TGG - Chr4:77111195-77111214 None:intergenic 25.0%
! ATTAACTTTGTAACTGTTGT+TGG - Chr4:77110387-77110406 None:intergenic 25.0%
! CAAAGAAAAAGAATTGTTTC+TGG + Chr4:77111275-77111294 MsG0480022822.01.T01:intron 25.0%
! GAATCAAAGACTAAAAATGT+TGG + Chr4:77109834-77109853 MsG0480022822.01.T01:five_prime_UTR 25.0%
! TAACTAACAAACTAACTAAC+CGG - Chr4:77111862-77111881 None:intergenic 25.0%
! TTGATTGAATTTATAGTGCT+TGG + Chr4:77111010-77111029 MsG0480022822.01.T01:intron 25.0%
! TTTAAACTACCATGTACAAT+AGG - Chr4:77110901-77110920 None:intergenic 25.0%
!! TTGACTTTGACTTATAAAGT+TGG - Chr4:77109940-77109959 None:intergenic 25.0%
!!! AACAGACTTTTAGTTATCTT+AGG + Chr4:77111195-77111214 MsG0480022822.01.T01:intron 25.0%
!!! GAGGTTGGTTTTTTTTATAA+GGG - Chr4:77110057-77110076 None:intergenic 25.0%
!!! GTTTTTTTTATAAGGGAAGT+GGG - Chr4:77110050-77110069 None:intergenic 25.0%
!!! TCTGATTTGTTTTTAAACAG+TGG + Chr4:77111946-77111965 MsG0480022822.01.T01:intron 25.0%
!!! TGAGGTTGGTTTTTTTTATA+AGG - Chr4:77110058-77110077 None:intergenic 25.0%
!!! TTTGATTTGTGTGATGATAT+TGG + Chr4:77111521-77111540 MsG0480022822.01.T01:intron 25.0%
!!! TTTTTTTTATAAGGGAAGTG+GGG - Chr4:77110049-77110068 None:intergenic 25.0%
AAGAAAAAGAACAAAGATGC+AGG - Chr4:77112099-77112118 None:intergenic 30.0%
AGAAAAAGAACAAAGATGCA+GGG - Chr4:77112098-77112117 None:intergenic 30.0%
AGAAGAAGAATAGTCTATGA+TGG - Chr4:77109907-77109926 None:intergenic 30.0%
AGACAAATAAGATGAGAAAG+TGG - Chr4:77112057-77112076 None:intergenic 30.0%
AGATGCTAATATATAACTGC+TGG + Chr4:77110650-77110669 MsG0480022822.01.T01:intron 30.0%
AGCAGTTATATATTAGCATC+TGG - Chr4:77110651-77110670 None:intergenic 30.0%
AGCTGTTAGTTGGTTATATT+AGG + Chr4:77110934-77110953 MsG0480022822.01.T01:intron 30.0%
ATGTATGGTCATTTATACAG+TGG + Chr4:77111355-77111374 MsG0480022822.01.T01:intron 30.0%
CAAACCGACCAATTATTTAT+CGG - Chr4:77111702-77111721 None:intergenic 30.0%
GAGTATCAAAAGTGATTCAT+CGG + Chr4:77111557-77111576 MsG0480022822.01.T01:CDS 30.0%
GTGTAGAAATATGTTGCATT+TGG - Chr4:77111600-77111619 None:intergenic 30.0%
TATGCACATATTGTTCCAAT+GGG + Chr4:77110737-77110756 MsG0480022822.01.T01:CDS 30.0%
TCTTCTATTTAGTGCTTGTT+TGG + Chr4:77112203-77112222 MsG0480022822.01.T01:three_prime_UTR 30.0%
TCTTTCAATTACGGTAACAT+GGG + Chr4:77111058-77111077 MsG0480022822.01.T01:intron 30.0%
TGAATTCACATTATGAGTTG+AGG - Chr4:77110013-77110032 None:intergenic 30.0%
TTATGCACATATTGTTCCAA+TGG + Chr4:77110736-77110755 MsG0480022822.01.T01:CDS 30.0%
TTCTTTCAATTACGGTAACA+TGG + Chr4:77111057-77111076 MsG0480022822.01.T01:intron 30.0%
TTGAATTTATAGTGCTTGGT+AGG + Chr4:77111014-77111033 MsG0480022822.01.T01:intron 30.0%
TTGTAAGAAATTACCTGGAA+GGG - Chr4:77110876-77110895 None:intergenic 30.0%
TTTCTGTTCTTTCTACTGTT+TGG - Chr4:77110105-77110124 None:intergenic 30.0%
!!! CATTATGAGTTGAGGTTTTT+TGG - Chr4:77110005-77110024 None:intergenic 30.0%
!!! GGTTATATTAGGCTTTGTTT+TGG + Chr4:77110945-77110964 MsG0480022822.01.T01:intron 30.0%
!!! GGTTTTTTTTATAAGGGAAG+TGG - Chr4:77110051-77110070 None:intergenic 30.0%
!!! TTTTTTTATAAGGGAAGTGG+GGG - Chr4:77110048-77110067 None:intergenic 30.0%
AAACAAGTCACATAGATGCA+GGG - Chr4:77109860-77109879 None:intergenic 35.0%
AAATGTAAATGCGACTCTAC+TGG + Chr4:77111636-77111655 MsG0480022822.01.T01:CDS 35.0%
AATATGTTGCATTTGGCACA+AGG - Chr4:77111593-77111612 None:intergenic 35.0%
AGGAGTTGTAAGAAATTACC+TGG - Chr4:77110881-77110900 None:intergenic 35.0%
ATGCACATATTGTTCCAATG+GGG + Chr4:77110738-77110757 MsG0480022822.01.T01:CDS 35.0%
CAACAACAACTTACAATGTG+AGG + Chr4:77111662-77111681 MsG0480022822.01.T01:CDS 35.0%
CTTACAACTCCTATTGTACA+TGG + Chr4:77110889-77110908 MsG0480022822.01.T01:intron 35.0%
GTTGTAAGAAATTACCTGGA+AGG - Chr4:77110877-77110896 None:intergenic 35.0%
TCATTTATACAGTGGTCCTA+AGG + Chr4:77111363-77111382 MsG0480022822.01.T01:intron 35.0%
TGTAAGAAATTACCTGGAAG+GGG - Chr4:77110875-77110894 None:intergenic 35.0%
! AGTTGTGATTAGCTGTTAGT+TGG + Chr4:77110924-77110943 MsG0480022822.01.T01:intron 35.0%
! TGAAGTATCAACAGATCCAA+CGG + Chr4:77110614-77110633 MsG0480022822.01.T01:intron 35.0%
!!! TGAGTTGAGGTTTTTTGGTT+TGG - Chr4:77110000-77110019 None:intergenic 35.0%
!!! TTGAGGTTTTTTGGTTTGGA+TGG - Chr4:77109996-77110015 None:intergenic 35.0%
AACACCAAAACATGAGCGTA+GGG + Chr4:77110129-77110148 MsG0480022822.01.T01:exon 40.0%
AAGGAAATAGCAACCCTGTT+CGG + Chr4:77110311-77110330 MsG0480022822.01.T01:CDS 40.0%
AATAGCGAGGGTTAAGTTTG+CGG + Chr4:77110585-77110604 MsG0480022822.01.T01:CDS 40.0%
ACAAGGCAAAGGAATCCAAA+TGG - Chr4:77110681-77110700 None:intergenic 40.0%
ACCCAAGTACAAGATTGTAC+CGG + Chr4:77110546-77110565 MsG0480022822.01.T01:CDS 40.0%
ATCTTTGTGTGTGGTGATTG+AGG - Chr4:77110076-77110095 None:intergenic 40.0%
CAAACAAGTCACATAGATGC+AGG - Chr4:77109861-77109880 None:intergenic 40.0%
CAATCACCACACACAAAGAT+AGG + Chr4:77110076-77110095 MsG0480022822.01.T01:five_prime_UTR 40.0%
CAATCTTGTACTTGGGTACA+TGG - Chr4:77110543-77110562 None:intergenic 40.0%
CTAACATTGACCGTTTCAAC+AGG + Chr4:77110508-77110527 MsG0480022822.01.T01:intron 40.0%
CTTAGGATGATCTCTTTCAC+CGG + Chr4:77111212-77111231 MsG0480022822.01.T01:intron 40.0%
GATTGAATACTACTCCCCAT+TGG - Chr4:77110755-77110774 None:intergenic 40.0%
GTTCCGTTACTACATTTGCA+GGG - Chr4:77110437-77110456 None:intergenic 40.0%
GTTGGATGTACCTGTTGAAA+CGG - Chr4:77110521-77110540 None:intergenic 40.0%
TAATCCCAACCAAGTCATCA+AGG + Chr4:77110403-77110422 MsG0480022822.01.T01:CDS 40.0%
TGGTTTCCTATCTTTGTGTG+TGG - Chr4:77110085-77110104 None:intergenic 40.0%
TGTTCCGTTACTACATTTGC+AGG - Chr4:77110438-77110457 None:intergenic 40.0%
TTACTACATTTGCAGGGGAA+TGG - Chr4:77110431-77110450 None:intergenic 40.0%
TTATCACAGCTACAAGGCAA+AGG - Chr4:77110692-77110711 None:intergenic 40.0%
TTCCCCTGCAAATGTAGTAA+CGG + Chr4:77110431-77110450 MsG0480022822.01.T01:CDS 40.0%
TTCCGTTACTACATTTGCAG+GGG - Chr4:77110436-77110455 None:intergenic 40.0%
TTGCAGTAGTAGCAAGTGTT+GGG + Chr4:77110183-77110202 MsG0480022822.01.T01:CDS 40.0%
TTTGCCTTGTAGCTGTGATA+AGG + Chr4:77110691-77110710 MsG0480022822.01.T01:intron 40.0%
! AACACTTGCTACTACTGCAA+CGG - Chr4:77110183-77110202 None:intergenic 40.0%
! AGACACTTGAAGTTTGCTTC+TGG - Chr4:77110230-77110249 None:intergenic 40.0%
! AGTGTTCTGAGTAGTAAGAG+TGG - Chr4:77111835-77111854 None:intergenic 40.0%
! GACCAGCTTGAAGCTTTTTA+GGG - Chr4:77110838-77110857 None:intergenic 40.0%
! GTCTATGATGGTGATGAAAG+AGG - Chr4:77109895-77109914 None:intergenic 40.0%
! TGACCAGCTTGAAGCTTTTT+AGG - Chr4:77110839-77110858 None:intergenic 40.0%
! TGCAAATGTAGTAACGGAAC+AGG + Chr4:77110437-77110456 MsG0480022822.01.T01:CDS 40.0%
! TTTTAAACAGTGGCACCTAG+TGG + Chr4:77111956-77111975 MsG0480022822.01.T01:intron 40.0%
!! GAAGTGTTTTACACCGAACA+GGG - Chr4:77110327-77110346 None:intergenic 40.0%
!! TAACTGTTGTTGGTGTTGCT+TGG - Chr4:77110377-77110396 None:intergenic 40.0%
AAACACTTCCTAGACCTCCT+TGG + Chr4:77110338-77110357 MsG0480022822.01.T01:CDS 45.0%
ACAAGATTGTACCGGGTGAT+GGG + Chr4:77110554-77110573 MsG0480022822.01.T01:CDS 45.0%
ACCCGGTACAATCTTGTACT+TGG - Chr4:77110551-77110570 None:intergenic 45.0%
ACTACTCAGAACACTTGTGC+CGG + Chr4:77111840-77111859 MsG0480022822.01.T01:CDS 45.0%
ATAGCGAGGGTTAAGTTTGC+GGG + Chr4:77110586-77110605 MsG0480022822.01.T01:CDS 45.0%
CACAAACGCAACAACCTTGA+AGG + Chr4:77110292-77110311 MsG0480022822.01.T01:CDS 45.0%
CCCAAGTACAAGATTGTACC+GGG + Chr4:77110547-77110566 MsG0480022822.01.T01:CDS 45.0%
CCCGGTACAATCTTGTACTT+GGG - Chr4:77110550-77110569 None:intergenic 45.0%
CGTACAGTTTGTGATTACGC+TGG + Chr4:77111786-77111805 MsG0480022822.01.T01:CDS 45.0%
CTTGTACTTGGGTACATGGT+TGG - Chr4:77110539-77110558 None:intergenic 45.0%
GAACACCAAAACATGAGCGT+AGG + Chr4:77110128-77110147 MsG0480022822.01.T01:exon 45.0%
GACCCTAAAAAGCTTCAAGC+TGG + Chr4:77110833-77110852 MsG0480022822.01.T01:CDS 45.0%
GGAACAAGATGGTCACTGTA+TGG + Chr4:77110150-77110169 MsG0480022822.01.T01:CDS 45.0%
GTAGCTGTGATAAGGTGGAT+GGG + Chr4:77110699-77110718 MsG0480022822.01.T01:intron 45.0%
GTTCCGGTGATTCAGATTCA+GGG + Chr4:77111978-77111997 MsG0480022822.01.T01:CDS 45.0%
GTTGCAGTAGTAGCAAGTGT+TGG + Chr4:77110182-77110201 MsG0480022822.01.T01:CDS 45.0%
GTTGCATTTGGCACAAGGAA+TGG - Chr4:77111588-77111607 None:intergenic 45.0%
TACAAGATTGTACCGGGTGA+TGG + Chr4:77110553-77110572 MsG0480022822.01.T01:CDS 45.0%
TCCACCTTATCACAGCTACA+AGG - Chr4:77110698-77110717 None:intergenic 45.0%
TCTAGACACCATAACTCTCC+TGG - Chr4:77109816-77109835 None:intergenic 45.0%
TGTAGCTGTGATAAGGTGGA+TGG + Chr4:77110698-77110717 MsG0480022822.01.T01:intron 45.0%
TTCCGGTGATTCAGATTCAG+GGG + Chr4:77111979-77111998 MsG0480022822.01.T01:CDS 45.0%
TTGGAAGGTTGTTAGCTCCA+AGG - Chr4:77110358-77110377 None:intergenic 45.0%
TTGTGTGTGGTGATTGAGGT+TGG - Chr4:77110072-77110091 None:intergenic 45.0%
! GCATCTAGGAGCATGTTGAA+TGG + Chr4:77112001-77112020 MsG0480022822.01.T01:CDS 45.0%
! GGAATGGAACCTTGATGACT+TGG - Chr4:77110415-77110434 None:intergenic 45.0%
! TAGGAGCATGTTGAATGGCT+GGG + Chr4:77112006-77112025 MsG0480022822.01.T01:CDS 45.0%
! TCTGGAATCTTATTCGCCGT+TGG - Chr4:77110633-77110652 None:intergenic 45.0%
! TGTTCCCTACGCTCATGTTT+TGG - Chr4:77110136-77110155 None:intergenic 45.0%
!! ATTCAGATTCAGGGGCATCT+AGG + Chr4:77111987-77112006 MsG0480022822.01.T01:CDS 45.0%
!! CAGGGTTGCTATTTCCTTCA+AGG - Chr4:77110309-77110328 None:intergenic 45.0%
!! GATGGAAAGCATTAGCAGTG+TGG - Chr4:77109978-77109997 None:intergenic 45.0%
!! GGAACCTTGATGACTTGGTT+GGG - Chr4:77110410-77110429 None:intergenic 45.0%
!! GGTTCTGAAGCTATCGTATC+CGG - Chr4:77111234-77111253 None:intergenic 45.0%
!! TAACTGCTGGTGCTACCATT+TGG + Chr4:77110663-77110682 MsG0480022822.01.T01:intron 45.0%
!! TGGAACCTTGATGACTTGGT+TGG - Chr4:77110411-77110430 None:intergenic 45.0%
!! TGTTGTTGGTGTTGCTTGGA+AGG - Chr4:77110373-77110392 None:intergenic 45.0%
!!! GGAAGTGTTTTACACCGAAC+AGG - Chr4:77110328-77110347 None:intergenic 45.0%
CAAGGTTGTTGCGTTTGTGC+TGG - Chr4:77110291-77110310 None:intergenic 50.0%
CATGAGCGTAGGGAACAAGA+TGG + Chr4:77110139-77110158 MsG0480022822.01.T01:exon 50.0%
CTTCTTGATGTTCCCCTTCC+AGG + Chr4:77110860-77110879 MsG0480022822.01.T01:CDS 50.0%
GAAGGTTGTTAGCTCCAAGG+AGG - Chr4:77110355-77110374 None:intergenic 50.0%
GAGGGTTAAGTTTGCGGGTT+TGG + Chr4:77110591-77110610 MsG0480022822.01.T01:CDS 50.0%
GCCTTGTAGCTGTGATAAGG+TGG + Chr4:77110694-77110713 MsG0480022822.01.T01:intron 50.0%
GGTTCCGGTGATTCAGATTC+AGG + Chr4:77111977-77111996 MsG0480022822.01.T01:CDS 50.0%
TCTGAATCACCGGAACCACT+AGG - Chr4:77111974-77111993 None:intergenic 50.0%
TGCCCCTGAATCTGAATCAC+CGG - Chr4:77111984-77112003 None:intergenic 50.0%
TGTACGACTACACGAAGCTG+TGG - Chr4:77111772-77111791 None:intergenic 50.0%
TGTCCGTCTATGGAATGCTC+CGG + Chr4:77111720-77111739 MsG0480022822.01.T01:CDS 50.0%
TGTTAGCTCCAAGGAGGTCT+AGG - Chr4:77110349-77110368 None:intergenic 50.0%
! CTAGGAGCATGTTGAATGGC+TGG + Chr4:77112005-77112024 MsG0480022822.01.T01:CDS 50.0%
! TGCGTTTGTGCTGGTGTAGT+CGG - Chr4:77110282-77110301 None:intergenic 50.0%
! TTGGGCTCTAGCATGCTTCT+CGG - Chr4:77111255-77111274 None:intergenic 50.0%
!! TTGGTCGGTTTGTCCGTCTA+TGG + Chr4:77111710-77111729 MsG0480022822.01.T01:CDS 50.0%
ACAGTGGCACCTAGTGGTTC+CGG + Chr4:77111962-77111981 MsG0480022822.01.T01:intron 55.0%
ATTGCATCCAGCCCATCACC+CGG - Chr4:77110568-77110587 None:intergenic 55.0%
GTCCGTCTATGGAATGCTCC+GGG + Chr4:77111721-77111740 MsG0480022822.01.T01:CDS 55.0%
GTCGGCTAAACTACGACACG+TGG - Chr4:77110264-77110283 None:intergenic 55.0%
GTTCCCAAGCATCACACTCC+CGG - Chr4:77111742-77111761 None:intergenic 55.0%
! CTCCCGGAGCATTCCATAGA+CGG - Chr4:77111726-77111745 None:intergenic 55.0%
! TATAAGGGAAGTGGGGGCGT+TGG - Chr4:77110042-77110061 None:intergenic 55.0%
! TGGGCTGGATGCAATAGCGA+GGG + Chr4:77110573-77110592 MsG0480022822.01.T01:CDS 55.0%
!! ATAAGGGAAGTGGGGGCGTT+GGG - Chr4:77110041-77110060 None:intergenic 55.0%
!! ATGGGCTGGATGCAATAGCG+AGG + Chr4:77110572-77110591 MsG0480022822.01.T01:CDS 55.0%
! GCTCCGGGAGTGTGATGCTT+GGG + Chr4:77111736-77111755 MsG0480022822.01.T01:CDS 60.0%
! TGCTCCGGGAGTGTGATGCT+TGG + Chr4:77111735-77111754 MsG0480022822.01.T01:CDS 60.0%
!! GATTGTACCGGGTGATGGGC+TGG + Chr4:77110558-77110577 MsG0480022822.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 77109811 77112350 77109811 ID=MsG0480022822.01;Name=MsG0480022822.01
Chr4 mRNA 77109811 77112350 77109811 ID=MsG0480022822.01.T01;Parent=MsG0480022822.01;Name=MsG0480022822.01.T01;_AED=0.20;_eAED=0.20;_QI=329|0.75|0.6|1|1|1|5|263|335
Chr4 exon 77109811 77110458 77109811 ID=MsG0480022822.01.T01:exon:19928;Parent=MsG0480022822.01.T01
Chr4 exon 77110530 77110612 77110530 ID=MsG0480022822.01.T01:exon:19929;Parent=MsG0480022822.01.T01
Chr4 exon 77110713 77110881 77110713 ID=MsG0480022822.01.T01:exon:19930;Parent=MsG0480022822.01.T01
Chr4 exon 77111547 77111861 77111547 ID=MsG0480022822.01.T01:exon:19931;Parent=MsG0480022822.01.T01
Chr4 exon 77111966 77112350 77111966 ID=MsG0480022822.01.T01:exon:19932;Parent=MsG0480022822.01.T01
Chr4 five_prime_UTR 77109811 77110139 77109811 ID=MsG0480022822.01.T01:five_prime_utr;Parent=MsG0480022822.01.T01
Chr4 CDS 77110140 77110458 77110140 ID=MsG0480022822.01.T01:cds;Parent=MsG0480022822.01.T01
Chr4 CDS 77110530 77110612 77110530 ID=MsG0480022822.01.T01:cds;Parent=MsG0480022822.01.T01
Chr4 CDS 77110713 77110881 77110713 ID=MsG0480022822.01.T01:cds;Parent=MsG0480022822.01.T01
Chr4 CDS 77111547 77111861 77111547 ID=MsG0480022822.01.T01:cds;Parent=MsG0480022822.01.T01
Chr4 CDS 77111966 77112087 77111966 ID=MsG0480022822.01.T01:cds;Parent=MsG0480022822.01.T01
Chr4 three_prime_UTR 77112088 77112350 77112088 ID=MsG0480022822.01.T01:three_prime_utr;Parent=MsG0480022822.01.T01
Gene Sequence

>MsG0480022822.01.T01

ATGAGCGTAGGGAACAAGATGGTCACTGTATGGTTAATCACCGTTGCAGTAGTAGCAAGTGTTGGGTTTACACTAACAGAAGCTCAACCAGAAGCAAACTTCAAGTGTCTAACAAAAAACGCCACGTGTCGTAGTTTAGCCGACTACACCAGCACAAACGCAACAACCTTGAAGGAAATAGCAACCCTGTTCGGTGTAAAACACTTCCTAGACCTCCTTGGAGCTAACAACCTTCCAAGCAACACCAACAACAGTTACAAAGTTAATCCCAACCAAGTCATCAAGGTTCCATTCCCCTGCAAATGTAGTAACGGAACAGGTACATCCAACCATGTACCCAAGTACAAGATTGTACCGGGTGATGGGCTGGATGCAATAGCGAGGGTTAAGTTTGCGGGTTTGGTGGATGGGAGCAGTGTGATGCATTATGCACATATTGTTCCAATGGGGAGTAGTATTCAATCTATTGCTCAAGAATATAATACTAGTCAACAGACTCTGCTGTCACTCAACGATCTTGATGACCCTAAAAAGCTTCAAGCTGGTCAGCTTCTTGATGTTCCCCTTCCAGTTTGTAACTCGAGTATCAAAAGTGATTCATCGGATTTTCCATTCCTTGTGCCAAATGCAACATATTTCTACACTGCAAATGAATGTGTCAAATGTAAATGCGACTCTACTGGTGTCAACAACAACTTACAATGTGAGGCATCAAATCTAAAACCGATAAATAATTGGTCGGTTTGTCCGTCTATGGAATGCTCCGGGAGTGTGATGCTTGGGAACACTGCATCCACAGCTTCGTGTAGTCGTACAGTTTGTGATTACGCTGGTTATACTTCTCGCAATATCTCTACCACTCTTACTACTCAGAACACTTGTGCCGTGGCACCTAGTGGTTCCGGTGATTCAGATTCAGGGGCATCTAGGAGCATGTTGAATGGCTGGGTTTTTAATAAGCTTCTAATTCTCATCCACTTTCTCATCTTATTTGTCTATCTATTGTAG
Protein sequence

>MsG0480022822.01.T01

MSVGNKMVTVWLITVAVVASVGFTLTEAQPEANFKCLTKNATCRSLADYTSTNATTLKEIATLFGVKHFLDLLGANNLPSNTNNSYKVNPNQVIKVPFPCKCSNGTGTSNHVPKYKIVPGDGLDAIARVKFAGLVDGSSVMHYAHIVPMGSSIQSIAQEYNTSQQTLLSLNDLDDPKKLQAGQLLDVPLPVCNSSIKSDSSDFPFLVPNATYFYTANECVKCKCDSTGVNNNLQCEASNLKPINNWSVCPSMECSGSVMLGNTASTASCSRTVCDYAGYTSRNISTTLTTQNTCAVAPSGSGDSDSGASRSMLNGWVFNKLLILIHFLILFVYLL*