Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000738.01.T01 | XP_013465713.1 | 96.501 | 343 | 6 | 3 | 1 | 340 | 1 | 340 | 0 | 669 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000738.01.T01 | A0A072VCI7 | 96.501 | 343 | 6 | 3 | 1 | 340 | 1 | 340 | 0.0 | 669 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048036.01 | MsG0180000738.01 | 0.823328 | 1.469519e-53 | 1.261316e-50 |
| MsG0080048347.01 | MsG0180000738.01 | 0.838011 | 3.703456e-57 | 4.896976e-54 |
| MsG0080048352.01 | MsG0180000738.01 | 0.832936 | 7.112882e-56 | 8.069214e-53 |
| MsG0180000738.01 | MsG0180000929.01 | 0.817573 | 3.074991e-52 | 2.247605e-49 |
| MsG0180000738.01 | MsG0180001513.01 | 0.805794 | 1.123805e-49 | 6.000313e-47 |
| MsG0180000738.01 | MsG0180001533.01 | 0.835587 | 1.538956e-56 | 1.890222e-53 |
| MsG0180000738.01 | MsG0180001691.01 | 0.820118 | 8.123067e-53 | 6.371533e-50 |
| MsG0180000738.01 | MsG0180002935.01 | 0.815810 | 7.638117e-52 | 5.320158e-49 |
| MsG0180000738.01 | MsG0180003235.01 | 0.854638 | 1.077188e-61 | 2.421666e-58 |
| MsG0180000738.01 | MsG0180004203.01 | 0.830308 | 3.159064e-55 | 3.315740e-52 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000738.01.T01 | MTR_1g009613 | 96.501 | 343 | 6 | 3 | 1 | 340 | 1 | 340 | 0.0 | 669 |
| MsG0180000738.01.T01 | MTR_0229s0060 | 96.694 | 242 | 5 | 2 | 1 | 242 | 1 | 239 | 4.25e-167 | 465 |
| MsG0180000738.01.T01 | MTR_4g127040 | 49.858 | 351 | 95 | 11 | 1 | 319 | 1 | 302 | 1.78e-84 | 258 |
| MsG0180000738.01.T01 | MTR_4g094908 | 39.141 | 396 | 127 | 13 | 1 | 340 | 1 | 338 | 7.97e-65 | 208 |
| MsG0180000738.01.T01 | MTR_3g108960 | 63.804 | 163 | 50 | 5 | 170 | 328 | 222 | 379 | 1.08e-60 | 200 |
| MsG0180000738.01.T01 | MTR_5g016610 | 56.934 | 137 | 49 | 3 | 208 | 340 | 141 | 271 | 1.82e-48 | 164 |
| MsG0180000738.01.T01 | MTR_0117s0040 | 64.557 | 79 | 28 | 0 | 241 | 319 | 53 | 131 | 1.62e-32 | 122 |
| MsG0180000738.01.T01 | MTR_2g016770 | 56.471 | 85 | 34 | 2 | 240 | 321 | 10 | 94 | 2.60e-27 | 107 |
| MsG0180000738.01.T01 | MTR_2g016770 | 57.692 | 78 | 32 | 1 | 245 | 321 | 9 | 86 | 5.60e-27 | 105 |
| MsG0180000738.01.T01 | MTR_8g087000 | 54.545 | 88 | 31 | 2 | 241 | 319 | 46 | 133 | 2.71e-26 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000738.01.T01 | AT4G31550 | 59.155 | 355 | 97 | 13 | 1 | 339 | 1 | 323 | 4.34e-124 | 359 |
| MsG0180000738.01.T01 | AT4G31550 | 58.708 | 356 | 98 | 13 | 1 | 339 | 1 | 324 | 5.00e-123 | 357 |
| MsG0180000738.01.T01 | AT2G24570 | 58.523 | 352 | 101 | 15 | 1 | 339 | 1 | 320 | 6.55e-113 | 331 |
| MsG0180000738.01.T01 | AT4G31550 | 55.068 | 296 | 88 | 11 | 1 | 282 | 1 | 265 | 8.85e-88 | 265 |
| MsG0180000738.01.T01 | AT4G24240 | 43.175 | 359 | 147 | 14 | 1 | 321 | 1 | 340 | 1.55e-65 | 211 |
| MsG0180000738.01.T01 | AT2G23320 | 38.701 | 354 | 129 | 11 | 1 | 321 | 1 | 299 | 1.39e-60 | 197 |
| MsG0180000738.01.T01 | AT3G04670 | 36.919 | 344 | 159 | 12 | 11 | 320 | 1 | 320 | 1.28e-53 | 179 |
| MsG0180000738.01.T01 | AT2G30590 | 53.757 | 173 | 64 | 6 | 154 | 319 | 207 | 370 | 7.91e-52 | 176 |
| MsG0180000738.01.T01 | AT5G28650 | 35.362 | 345 | 163 | 12 | 11 | 320 | 1 | 320 | 2.16e-48 | 166 |
| MsG0180000738.01.T01 | AT4G24240 | 38.486 | 317 | 144 | 13 | 1 | 282 | 1 | 301 | 1.62e-37 | 136 |
Find 120 sgRNAs with CRISPR-Local
Find 236 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGACGGCCGATATACCAC+CGG | 0.676381 | 1:-10466790 | MsG0180000738.01.T01:CDS |
| AAATACCAAACCCACAATCC+CGG | 0.560277 | 1:+10466466 | None:intergenic |
| AAATATGGCAGTGGAACTCA+TGG | 0.506689 | 1:-10469041 | MsG0180000738.01.T01:exon |
| AAATCGAGTGTCATGCTGTG+TGG | 0.710920 | 1:+10468641 | None:intergenic |
| AAATTGTTGAAGAGTTAAAA+TGG | 0.319358 | 1:+10469090 | None:intergenic |
| AACACTCCGATGACGTTTCA+GGG | 0.483071 | 1:-10468361 | MsG0180000738.01.T01:CDS |
| AACCGGGGCGCGTCTGAACC+GGG | 0.500032 | 1:+10468838 | None:intergenic |
| AACCGGTGGTGATGAAGATG+AGG | 0.659341 | 1:+10468808 | None:intergenic |
| AAGAAAAGGTGTCATGATCA+TGG | 0.525519 | 1:-10468386 | MsG0180000738.01.T01:CDS |
| AAGATGAGGAGGAATGAACC+GGG | 0.573874 | 1:+10468822 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACGTTAATGCATGTAATC+GGG | + | Chr1:10467549-10467568 | None:intergenic | 30.0% | |
| AAAGAAAGGGAAAAGGTTAA+TGG | - | Chr1:10467679-10467698 | MsG0180000738.01.T01:intron | 30.0% | |
| AAAGTCAACATAATTAACGG+TGG | + | Chr1:10468715-10468734 | None:intergenic | 30.0% | |
| AAGTTAAGTTAGACCTAGAT+TGG | - | Chr1:10468288-10468307 | MsG0180000738.01.T01:intron | 30.0% | |
| ACTCTTATCCAATTGGAATA+TGG | - | Chr1:10468386-10468405 | MsG0180000738.01.T01:CDS | 30.0% | |
| AGGGGAATTAAAAAAATTGG+AGG | - | Chr1:10468963-10468982 | MsG0180000738.01.T01:CDS | 30.0% | |
| AGTATTTCCCATATTCCAAT+TGG | + | Chr1:10468397-10468416 | None:intergenic | 30.0% | |
| AGTCAAAGCAATTAAGTGAA+GGG | + | Chr1:10467937-10467956 | None:intergenic | 30.0% | |
| AGTTGAGTGTTTAATTGAGT+TGG | + | Chr1:10466472-10466491 | None:intergenic | 30.0% | |
| ATAGTGCACATTGCAAAAAT+TGG | - | Chr1:10467413-10467432 | MsG0180000738.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 10466250 | 10469164 | 10466250 | ID=MsG0180000738.01;Name=MsG0180000738.01 |
| Chr1 | mRNA | 10466250 | 10469164 | 10466250 | ID=MsG0180000738.01.T01;Parent=MsG0180000738.01;Name=MsG0180000738.01.T01;_AED=0.34;_eAED=0.34;_QI=105|0.5|0.66|1|1|1|3|204|340 |
| Chr1 | exon | 10468332 | 10469164 | 10468332 | ID=MsG0180000738.01.T01:exon:1292;Parent=MsG0180000738.01.T01 |
| Chr1 | exon | 10466732 | 10466848 | 10466732 | ID=MsG0180000738.01.T01:exon:1293;Parent=MsG0180000738.01.T01 |
| Chr1 | exon | 10466250 | 10466631 | 10466250 | ID=MsG0180000738.01.T01:exon:1294;Parent=MsG0180000738.01.T01 |
| Chr1 | five_prime_UTR | 10469060 | 10469164 | 10469060 | ID=MsG0180000738.01.T01:five_prime_utr;Parent=MsG0180000738.01.T01 |
| Chr1 | CDS | 10468332 | 10469059 | 10468332 | ID=MsG0180000738.01.T01:cds;Parent=MsG0180000738.01.T01 |
| Chr1 | CDS | 10466732 | 10466848 | 10466732 | ID=MsG0180000738.01.T01:cds;Parent=MsG0180000738.01.T01 |
| Chr1 | CDS | 10466454 | 10466631 | 10466454 | ID=MsG0180000738.01.T01:cds;Parent=MsG0180000738.01.T01 |
| Chr1 | three_prime_UTR | 10466250 | 10466453 | 10466250 | ID=MsG0180000738.01.T01:three_prime_utr;Parent=MsG0180000738.01.T01 |
| Gene Sequence |
>MsG0180000738.01.T01 ATGGCAGTGGAACTCATGGGGTTCCCAAAAATAGATGAACAAAAAGCCATACAAGAGGCTGCATCAGAAGGACTCAAAGGTATGGAACACTTGATCCTAACCCTTTCTCATCAACCAACTCAATTAAACACTCAACTAACCGACCACACCGTTTCTAAGTTCAAAAAACTCATCTCTTTACTTAACCGAACCGGTCACGCCCGGTTCAGACGCGCCCCGGTTCATTCCTCCTCATCTTCATCACCACCGGTTCAACCGGTTCAGATTCAATCCACTCCTTCACCGGTTCAAACACCAACCCTGACTCTACCAAAACACTTTCCTTCTCCGTCACCTGCACTGTCACCGGCGCCAATCTCCGTGCGTCACGCGCCGGCGAGCTTTGTTCAACCGCAACCACACAGCATGACACTCGATTTCACGAAGCCAAACGACGTCGTTTTGAGCTCAAACACGAAAAACTCCATGGTTGAGCTTGAATTCTCGAAGGATACAGCAACGTTTAGCGTTTCTTCAGCTTCATCGTTTATGTCATCTGCTATTACTGGTGATGGAAGCGTTAACGGGAAACAAGGGTCTTCGATTTTTCTCAACCCAGCGGCGACACCAGCTATCTCCGGCGGAAAACCGCCGCTATCGGCGGTGCCGTCTAAGAAAAGGTGTCATGATCATGGTGAACACTCCGATGACGTTTCAGGGTCGAATAAGTGCCACTGTGTCAAGAGAAGGGTGAAGAGGACTGTGAGAGTACCGGCGATAAGTTCAAAGACGGCCGATATACCACCGGACGAGTACTCGTGGCGGAAATACGGTCAGAAACCGATCAAGGGATCACCATATCCACGAGGCTATTACAAGTGTAGCACTGTAAGAGGGTGCCCAGCAAGGAAACACGTGGAGCGTGCAACAGATGATCCAACGATGCTGATTGTAACGTATGAAGGAGAGCACAGACATACTATTCAAGCTGCGATGCAGGAGAACGCATCCGGGATTGTGGGTTTGGTATTTGAGTCAACGTAA |
| Protein sequence |
>MsG0180000738.01.T01 MAVELMGFPKIDEQKAIQEAASEGLKGMEHLILTLSHQPTQLNTQLTDHTVSKFKKLISLLNRTGHARFRRAPVHSSSSSSPPVQPVQIQSTPSPVQTPTLTLPKHFPSPSPALSPAPISVRHAPASFVQPQPHSMTLDFTKPNDVVLSSNTKNSMVELEFSKDTATFSVSSASSFMSSAITGDGSVNGKQGSSIFLNPAATPAISGGKPPLSAVPSKKRCHDHGEHSDDVSGSNKCHCVKRRVKRTVRVPAISSKTADIPPDEYSWRKYGQKPIKGSPYPRGYYKCSTVRGCPARKHVERATDDPTMLIVTYEGEHRHTIQAAMQENASGIVGLVFEST* |
