AlfalfaGEDB | MsG0480022843.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022843.01

KEGG: -
GO: GO:0003676
PFAM: PF13456

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022843.01.T01	XP_024626708.2	61.644	73	27	1	12	83	158	230	5.99E-21	94.4

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022843.01.T01	A0A396GZG8	63.014	73	26	1	12	83	130	202	2.97e-19	88.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0480022843.01	MsG0780041116.01	PPI
MsG0480022843.01	MsG0780041116.01	PPI
MsG0480022843.01	MsG0780040274.01	PPI
MsG0480022843.01	MsG0280009322.01	PPI
MsG0480022843.01	MsG0380013578.01	PPI
MsG0480022843.01	MsG0280010449.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022843.01.T01	MTR_7g063280	50.685	73	35	1	12	83	75	147	7.54e-20	78.6
MsG0480022843.01.T01	MTR_8g036780	56.338	71	30	1	12	81	37	107	1.15e-19	76.6
MsG0480022843.01.T01	MTR_0093s0070	47.297	74	38	1	11	83	125	198	1.99e-19	78.2
MsG0480022843.01.T01	MTR_6g016265	52.055	73	34	1	12	83	42	114	3.37e-19	75.9
MsG0480022843.01.T01	MTR_0015s0120	59.677	62	24	1	12	72	296	357	4.48e-17	73.9
MsG0480022843.01.T01	MTR_8g098925	45.833	72	38	1	13	83	69	140	6.95e-17	70.9
MsG0480022843.01.T01	MTR_7g013520	43.210	81	45	1	4	83	65	145	1.11e-16	70.9
MsG0480022843.01.T01	MTR_8g059135	59.016	61	24	1	12	71	31	91	1.27e-16	69.3
MsG0480022843.01.T01	MTR_8g088220	53.030	66	30	1	19	83	4	69	1.42e-16	67.8
MsG0480022843.01.T01	MTR_6g089560	45.205	73	39	1	12	83	119	191	1.64e-16	71.2
MsG0480022843.01.T01	MTR_5g034450	49.275	69	34	1	4	71	44	112	3.21e-16	67.8
MsG0480022843.01.T01	MTR_6g034915	46.479	71	37	1	13	82	59	129	5.27e-16	68.9
MsG0480022843.01.T01	MTR_5g049680	46.988	83	40	3	4	83	26	107	5.67e-16	67.0
MsG0480022843.01.T01	MTR_7g053400	54.688	64	28	1	8	70	174	237	2.12e-15	68.6
MsG0480022843.01.T01	MTR_1g017840	44.444	72	39	1	13	83	82	153	2.38e-15	66.6
MsG0480022843.01.T01	MTR_6g453270	47.222	72	37	1	13	83	304	375	3.07e-15	68.9
MsG0480022843.01.T01	MTR_8g099580	50.000	72	35	1	13	83	210	281	3.49e-15	68.6
MsG0480022843.01.T01	MTR_3g116210	45.205	73	39	1	12	83	100	172	3.49e-15	67.0
MsG0480022843.01.T01	MTR_1g069475	45.205	73	39	1	12	83	167	239	4.37e-15	67.8
MsG0480022843.01.T01	MTR_1g050488	52.459	61	28	1	12	71	101	161	2.66e-14	63.9
MsG0480022843.01.T01	MTR_4g008270	41.096	73	42	1	12	83	13	85	3.72e-14	62.0
MsG0480022843.01.T01	MTR_5g091440	61.111	54	20	1	17	69	3	56	4.79e-14	62.8
MsG0480022843.01.T01	MTR_1g074090	44.286	70	38	1	4	72	76	145	1.64e-13	61.6
MsG0480022843.01.T01	MTR_1g088650	42.466	73	41	1	12	83	89	161	2.56e-13	62.0
MsG0480022843.01.T01	MTR_1g022215	51.852	54	25	1	12	64	17	70	3.05e-13	59.7
MsG0480022843.01.T01	MTR_5g036320	46.875	64	33	1	12	74	82	145	4.08e-13	60.5
MsG0480022843.01.T01	MTR_6g016135	53.333	60	27	1	13	71	383	442	4.66e-13	62.8
MsG0480022843.01.T01	MTR_1g081930	43.056	72	40	1	13	83	42	113	5.67e-13	60.1
MsG0480022843.01.T01	MTR_5g075610	48.529	68	32	2	15	81	1	66	1.83e-12	57.8
MsG0480022843.01.T01	MTR_6g016355	46.667	60	31	1	12	70	119	178	2.61e-12	59.3
MsG0480022843.01.T01	MTR_5g083200	45.205	73	39	1	12	83	101	173	2.64e-12	59.3
MsG0480022843.01.T01	MTR_0002s1220	45.205	73	39	1	12	83	101	173	2.64e-12	59.3
MsG0480022843.01.T01	MTR_3g111390	43.836	73	40	1	12	83	101	173	6.30e-12	58.5
MsG0480022843.01.T01	MTR_1g070820	43.836	73	40	1	12	83	101	173	6.30e-12	58.5
MsG0480022843.01.T01	MTR_2g059470	42.424	66	37	1	12	76	3	68	9.07e-12	55.8
MsG0480022843.01.T01	MTR_8g031800	41.096	73	42	1	12	83	34	106	9.30e-12	56.2
MsG0480022843.01.T01	MTR_1g045340	43.836	73	40	1	12	83	87	159	1.11e-11	57.8
MsG0480022843.01.T01	MTR_4g073820	53.704	54	24	1	15	68	1	53	1.65e-11	54.7
MsG0480022843.01.T01	MTR_1g034430	39.726	73	43	1	12	83	98	170	5.60e-11	55.8
MsG0480022843.01.T01	MTR_1g074240	45.161	62	33	1	12	72	3	64	7.97e-11	53.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 29 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATAGTTGCAGACAGCATATC+TGG	0.305528	4:-77413222	MsG0480022843.01.T01:CDS
GGATCTACAGCTGTGGAATA+TGG	0.343456	4:-77413265	MsG0480022843.01.T01:CDS
TGCTGACGGAGCCTTTGTTC+TGG	0.370192	4:-77413367	MsG0480022843.01.T01:CDS
GAGGGACACCCACTCAGTTC+TGG	0.382769	4:+77413344	None:intergenic
TATGCTGTCTGCAACTATCT+TGG	0.411848	4:+77413227	None:intergenic
CGCTGTTGCGTGCTATGGAA+TGG	0.427121	4:-77413293	MsG0480022843.01.T01:CDS
TTTGTGAAGTTCTGGATAGA+GGG	0.430142	4:-77413414	MsG0480022843.01.T01:CDS
GCTTGCGCTGTTGCGTGCTA+TGG	0.430369	4:-77413298	MsG0480022843.01.T01:CDS
TCCCTCTGCTTGAGTTCACT+AGG	0.472287	4:-77413327	MsG0480022843.01.T01:CDS
AGCATATCTGGGAAGCAGAT+TGG	0.487959	4:-77413210	MsG0480022843.01.T01:CDS
CCCTCTGCTTGAGTTCACTA+GGG	0.497690	4:-77413326	MsG0480022843.01.T01:CDS
TGCGTGCTATGGAATGGGTA+AGG	0.533283	4:-77413287	MsG0480022843.01.T01:CDS
TGTTCTGGCCAGAACTGAGT+GGG	0.538472	4:-77413352	MsG0480022843.01.T01:CDS
TCTATCCAGAACTTCACAAA+AGG	0.538602	4:+77413417	None:intergenic
GTGAAGTTCTGGATAGAGGG+GGG	0.554443	4:-77413411	MsG0480022843.01.T01:CDS
CTCAGTTCTGGCCAGAACAA+AGG	0.558263	4:+77413356	None:intergenic
TTGTGAAGTTCTGGATAGAG+GGG	0.561105	4:-77413413	MsG0480022843.01.T01:CDS
GCTGTTGCGTGCTATGGAAT+GGG	0.567726	4:-77413292	MsG0480022843.01.T01:CDS
CTGCTTGAGTTCACTAGGGG+AGG	0.585715	4:-77413322	MsG0480022843.01.T01:CDS
TTGTTCTGGCCAGAACTGAG+TGG	0.587383	4:-77413353	MsG0480022843.01.T01:CDS
TGTGAAGTTCTGGATAGAGG+GGG	0.588898	4:-77413412	MsG0480022843.01.T01:CDS
TAGTTGCAGACAGCATATCT+GGG	0.598673	4:-77413221	MsG0480022843.01.T01:CDS
CCCTAGTGAACTCAAGCAGA+GGG	0.605807	4:+77413326	None:intergenic
GTGGAATATGGACTTCGAAG+TGG	0.631563	4:-77413253	MsG0480022843.01.T01:CDS
GCGTGCTATGGAATGGGTAA+GGG	0.635545	4:-77413286	MsG0480022843.01.T01:CDS
CCTCTGCTTGAGTTCACTAG+GGG	0.640276	4:-77413325	MsG0480022843.01.T01:CDS
CCCCTAGTGAACTCAAGCAG+AGG	0.641934	4:+77413325	None:intergenic
GGGTAAGGGATCTACAGCTG+TGG	0.648809	4:-77413272	MsG0480022843.01.T01:CDS
ATTTGTATCAGAGATGCTGA+CGG	0.684660	4:-77413381	MsG0480022843.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATTTGTATCAGAGATGCTGA+CGG	-	Chr4:77413236-77413255	MsG0480022843.01.T01:CDS	35.0%
	TCTATCCAGAACTTCACAAA+AGG	+	Chr4:77413203-77413222	None:intergenic	35.0%
!!	TTTGTGAAGTTCTGGATAGA+GGG	-	Chr4:77413203-77413222	MsG0480022843.01.T01:CDS	35.0%
!!!	TTTTGTGAAGTTCTGGATAG+AGG	-	Chr4:77413202-77413221	MsG0480022843.01.T01:CDS	35.0%
	ATAGTTGCAGACAGCATATC+TGG	-	Chr4:77413395-77413414	MsG0480022843.01.T01:CDS	40.0%
	TAGTTGCAGACAGCATATCT+GGG	-	Chr4:77413396-77413415	MsG0480022843.01.T01:CDS	40.0%
	TATGCTGTCTGCAACTATCT+TGG	+	Chr4:77413393-77413412	None:intergenic	40.0%
!!	TTGTGAAGTTCTGGATAGAG+GGG	-	Chr4:77413204-77413223	MsG0480022843.01.T01:CDS	40.0%
	AGCATATCTGGGAAGCAGAT+TGG	-	Chr4:77413407-77413426	MsG0480022843.01.T01:CDS	45.0%
	GGATCTACAGCTGTGGAATA+TGG	-	Chr4:77413352-77413371	MsG0480022843.01.T01:CDS	45.0%
	GTGGAATATGGACTTCGAAG+TGG	-	Chr4:77413364-77413383	MsG0480022843.01.T01:CDS	45.0%
!	TGCGTCCTTTTGTGAAGTTC+TGG	-	Chr4:77413195-77413214	MsG0480022843.01.T01:CDS	45.0%
!!	TGTGAAGTTCTGGATAGAGG+GGG	-	Chr4:77413205-77413224	MsG0480022843.01.T01:CDS	45.0%
	CCCTAGTGAACTCAAGCAGA+GGG	+	Chr4:77413294-77413313	None:intergenic	50.0%
	CCCTCTGCTTGAGTTCACTA+GGG	-	Chr4:77413291-77413310	MsG0480022843.01.T01:CDS	50.0%
	CCTCTGCTTGAGTTCACTAG+GGG	-	Chr4:77413292-77413311	MsG0480022843.01.T01:CDS	50.0%
	GCGTGCTATGGAATGGGTAA+GGG	-	Chr4:77413331-77413350	MsG0480022843.01.T01:CDS	50.0%
	GCTGTTGCGTGCTATGGAAT+GGG	-	Chr4:77413325-77413344	MsG0480022843.01.T01:CDS	50.0%
	TCCCTCTGCTTGAGTTCACT+AGG	-	Chr4:77413290-77413309	MsG0480022843.01.T01:CDS	50.0%
	TGCGTGCTATGGAATGGGTA+AGG	-	Chr4:77413330-77413349	MsG0480022843.01.T01:CDS	50.0%
	TGTTCTGGCCAGAACTGAGT+GGG	-	Chr4:77413265-77413284	MsG0480022843.01.T01:CDS	50.0%
	TTGTTCTGGCCAGAACTGAG+TGG	-	Chr4:77413264-77413283	MsG0480022843.01.T01:CDS	50.0%
!	CTCAGTTCTGGCCAGAACAA+AGG	+	Chr4:77413264-77413283	None:intergenic	50.0%
!	TTCTGGATAGAGGGGGGTAT+CGG	-	Chr4:77413212-77413231	MsG0480022843.01.T01:CDS	50.0%
!!	GTGAAGTTCTGGATAGAGGG+GGG	-	Chr4:77413206-77413225	MsG0480022843.01.T01:CDS	50.0%
	CCCCTAGTGAACTCAAGCAG+AGG	+	Chr4:77413295-77413314	None:intergenic	55.0%
	CGCTGTTGCGTGCTATGGAA+TGG	-	Chr4:77413324-77413343	MsG0480022843.01.T01:CDS	55.0%
	CTGCTTGAGTTCACTAGGGG+AGG	-	Chr4:77413295-77413314	MsG0480022843.01.T01:CDS	55.0%
	GGGTAAGGGATCTACAGCTG+TGG	-	Chr4:77413345-77413364	MsG0480022843.01.T01:CDS	55.0%
	TGCTGACGGAGCCTTTGTTC+TGG	-	Chr4:77413250-77413269	MsG0480022843.01.T01:CDS	55.0%
	GAGGGACACCCACTCAGTTC+TGG	+	Chr4:77413276-77413295	None:intergenic	60.0%
	GCTTGCGCTGTTGCGTGCTA+TGG	-	Chr4:77413319-77413338	MsG0480022843.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	77413194	77413445	77413194	ID=MsG0480022843.01;Name=MsG0480022843.01
Chr4	mRNA	77413194	77413445	77413194	ID=MsG0480022843.01.T01;Parent=MsG0480022843.01;Name=MsG0480022843.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|83
Chr4	exon	77413194	77413445	77413194	ID=MsG0480022843.01.T01:exon:4867;Parent=MsG0480022843.01.T01
Chr4	CDS	77413194	77413445	77413194	ID=MsG0480022843.01.T01:cds;Parent=MsG0480022843.01.T01

Gene Sequence	>MsG0480022843.01.T01 ATGCGTCCTTTTGTGAAGTTCTGGATAGAGGGGGGTATCGGTATTTGTATCAGAGATGCTGACGGAGCCTTTGTTCTGGCCAGAACTGAGTGGGTGTCCCTCTGCTTGAGTTCACTAGGGGAGGCGCTTGCGCTGTTGCGTGCTATGGAATGGGTAAGGGATCTACAGCTGTGGAATATGGACTTCGAAGTGGATTCCAAGATAGTTGCAGACAGCATATCTGGGAAGCAGATTGGTGTATCAGAATTTTAG
Protein sequence	>MsG0480022843.01.T01 MRPFVKFWIEGGIGICIRDADGAFVLARTEWVSLCLSSLGEALALLRAMEWVRDLQLWNMDFEVDSKIVADSISGKQIGVSEF*