AlfalfaGEDB | MsG0480022879.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022879.01

KEGG: -
GO: GO:0016491 GO:0055114
PFAM: PF03171 PF14226

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022879.01.T01	XP_039685275.1	76.404	356	48	3	1	321	1	355	0	550

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022879.01.T01	Q39224	45.714	350	150	5	5	316	11	358	2.62E-108	322

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022879.01.T01	A0A396GUN0	76.404	356	48	3	1	321	1	355	0.0	550

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480022879.01	MsG0780038263.01	0.814734	1.325181e-51	8.963016e-49

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022879.01.T01	MTR_5g048550	53.276	351	124	4	5	320	7	352	1.16e-126	366
MsG0480022879.01.T01	MTR_8g064200	54.467	347	119	4	5	316	12	354	1.64e-126	366
MsG0480022879.01.T01	MTR_8g064200	54.467	347	119	4	5	316	17	359	3.30e-126	365
MsG0480022879.01.T01	MTR_0009s0480	51.445	346	128	4	6	316	7	347	2.72e-125	363
MsG0480022879.01.T01	MTR_0009s0050	53.602	347	121	4	5	316	7	348	8.11e-125	362
MsG0480022879.01.T01	MTR_0009s0050	53.602	347	121	4	5	316	7	348	1.08e-124	361
MsG0480022879.01.T01	MTR_5g048740	52.273	352	127	5	1	316	1	347	1.17e-124	361
MsG0480022879.01.T01	MTR_5g048470	53.009	349	126	3	5	318	12	357	2.98e-124	360
MsG0480022879.01.T01	MTR_5g055900	51.862	349	128	4	5	318	7	350	8.28e-122	354
MsG0480022879.01.T01	MTR_5g055680	53.026	347	123	4	5	316	7	348	1.46e-121	353

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022879.01.T01	AT1G17020	45.714	350	150	5	5	316	11	358	2.67e-109	322
MsG0480022879.01.T01	AT1G78550	42.816	348	161	4	5	316	11	356	2.65e-102	304
MsG0480022879.01.T01	AT1G17010	45.429	350	151	6	5	316	11	358	1.21e-101	303
MsG0480022879.01.T01	AT4G25300	44.571	350	153	6	5	316	10	356	4.08e-100	299
MsG0480022879.01.T01	AT4G25310	44.160	351	150	8	5	316	10	353	4.75e-94	283
MsG0480022879.01.T01	AT1G78550	43.066	274	121	2	78	316	5	278	1.74e-81	248
MsG0480022879.01.T01	AT4G25310	39.542	349	141	7	5	316	10	325	1.31e-74	233
MsG0480022879.01.T01	AT3G21420	38.136	354	179	6	3	316	9	362	2.30e-72	228
MsG0480022879.01.T01	AT2G38240	35.057	348	182	5	10	318	11	353	2.07e-67	215
MsG0480022879.01.T01	AT4G25300	49.495	198	95	3	121	316	68	262	5.95e-66	208

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Find 78 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAACCCCATTCTTTGC+AGG	0.335772	4:+77991268	None:intergenic
AAATACCTCAGTTATTTCCT+TGG	0.398108	4:+77990028	None:intergenic
AAATCCCAAGGAAATAACTG+AGG	0.705336	4:-77990033	MsG0480022879.01.T01:CDS
AAATGAAATGAAGATCACAA+AGG	0.678483	4:-77989132	MsG0480022879.01.T01:CDS
AACATTGTTTGAACTTTCAA+TGG	0.372915	4:-77990866	MsG0480022879.01.T01:CDS
AAGAAGCTATGGCAGAAAGA+AGG	0.537844	4:-77990835	MsG0480022879.01.T01:CDS
AAGATCTTGGGACAACAATT+TGG	0.238023	4:+77991328	None:intergenic
AAGTCTCATTCTGATATTAA+TGG	0.272225	4:-77989933	MsG0480022879.01.T01:CDS
AATACCTCAGTTATTTCCTT+GGG	0.416470	4:+77990029	None:intergenic
AATATATCGCTTTGGAACAT+TGG	0.472518	4:+77991424	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAGGCCTTCAAATAAAAA+AGG	-	Chr4:77990547-77990566	MsG0480022879.01.T01:intron	30.0%
AGAGATATATTCTACAGAGT+TGG	-	Chr4:77990322-77990341	MsG0480022879.01.T01:intron	30.0%
AGGATAATAATTCACCCTTA+TGG	+	Chr4:77990441-77990460	None:intergenic	30.0%
ATTCTCTCTGCATTTGAATA+AGG	+	Chr4:77990300-77990319	None:intergenic	30.0%
CTCTTACAAGAACAGTTAAT+AGG	-	Chr4:77991339-77991358	MsG0480022879.01.T01:CDS	30.0%
CTGTTCTTCAGATAAAATGA+AGG	+	Chr4:77989641-77989660	None:intergenic	30.0%
GGAAAATCATATATTGGGAA+AGG	+	Chr4:77989319-77989338	None:intergenic	30.0%
TAGTTAATTCAGAGAAAGAG+AGG	-	Chr4:77991114-77991133	MsG0480022879.01.T01:intron	30.0%
TAGTTTAGTTGCTATAGTGA+TGG	-	Chr4:77990993-77991012	MsG0480022879.01.T01:intron	30.0%
TCATTTAAGCTGGTTAATCA+TGG	-	Chr4:77989496-77989515	MsG0480022879.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	77988914	77991535	77988914	ID=MsG0480022879.01;Name=MsG0480022879.01
Chr4	mRNA	77988914	77991535	77988914	ID=MsG0480022879.01.T01;Parent=MsG0480022879.01;Name=MsG0480022879.01.T01;_AED=0.49;_eAED=0.49;_QI=31\|0.75\|0.6\|1\|0.75\|0.6\|5\|179\|321
Chr4	exon	77988914	77988984	77988914	ID=MsG0480022879.01.T01:exon:11212;Parent=MsG0480022879.01.T01
Chr4	exon	77989013	77989378	77989013	ID=MsG0480022879.01.T01:exon:11211;Parent=MsG0480022879.01.T01
Chr4	exon	77989812	77990152	77989812	ID=MsG0480022879.01.T01:exon:11210;Parent=MsG0480022879.01.T01
Chr4	exon	77990818	77990944	77990818	ID=MsG0480022879.01.T01:exon:11209;Parent=MsG0480022879.01.T01
Chr4	exon	77991265	77991535	77991265	ID=MsG0480022879.01.T01:exon:11208;Parent=MsG0480022879.01.T01
Chr4	five_prime_UTR	77991505	77991535	77991505	ID=MsG0480022879.01.T01:five_prime_utr;Parent=MsG0480022879.01.T01
Chr4	CDS	77991265	77991504	77991265	ID=MsG0480022879.01.T01:cds;Parent=MsG0480022879.01.T01
Chr4	CDS	77990818	77990944	77990818	ID=MsG0480022879.01.T01:cds;Parent=MsG0480022879.01.T01
Chr4	CDS	77989812	77990152	77989812	ID=MsG0480022879.01.T01:cds;Parent=MsG0480022879.01.T01
Chr4	CDS	77989121	77989378	77989121	ID=MsG0480022879.01.T01:cds;Parent=MsG0480022879.01.T01
Chr4	three_prime_UTR	77989013	77989120	77989013	ID=MsG0480022879.01.T01:three_prime_utr;Parent=MsG0480022879.01.T01
Chr4	three_prime_UTR	77988914	77988984	77988914	ID=MsG0480022879.01.T01:three_prime_utr;Parent=MsG0480022879.01.T01

Gene Sequence

>MsG0480022879.01.T01

ATGGATATAAGATCTATTCTGGTGCCCTCTGTTCAAGAGCTAGCAAAGGAATCATTGACCAATGTTCCAAAGCGATATATTCTTCCAGATCAAGACTCTGTAGTACTATCTAACCCTACCTCTTCTTTACCTCAAGTTCCTGTCATTGATCTTGCCAAATTGTTGTCCCAAGATCTTAATCTCAAGGGACATGAGCTAGAGAAGCTACACTCTGCCTGCAAAGAATGGGGTTTCTTTCAGCTGGTTAATCATGGAGTAAGTTCTTCCTTAGTGGAAAATATGAAGAGAGGAGCTAAAACATTGTTTGAACTTTCAATGGAAGAAAAGAAGAAGCTATGGCAGAAAGAAGGAGACTTGGAGGGATTTGCCTTATTCAAATGCAGAGAGAATCTAGAGATATATTCTACAGAGTTGGAAAAACTCACCATCAAAATGCTCGAACTTATGGCAAATGCTCTTGCAATAAATCCCAAGGAAATAACTGAGGTATTTAACATAGGAACTCAAGCCATAAGGGTGAATTATTATCCTCCATGCCCCCAACCAGAACGAGTCATTGGCCTAAAGTCTCATTCTGATATTAATGGCCTCACCATCCTTCTTCAAATCAGCGATATAGAAGGCCTTCAAATAAAAAAGGATGGACAATGGATTCCTATTAAACCCATGCCTAATGCTTTCATCATCAATATTGGTGACATATTAGAGATTATAACGAATGGAATTTACCCAAGCATTGAGCATCGAGCAATAGTTAATTCAGAGAAAGAGAGGATATCCCTAGCTGCTTTTTATGCCCCTAACATGCAAGCAATGTTAGCTCCAGCACCAAGTCTTGTTACTCAAGAAAGACCAGCACAGTATAGAAGAATTAGTGTTGTTGATCACTTCAATGGCTACTTTTCACAAGAGCTCCGTGGAAAATCATATCTAAATGAAATGAAGATCACAAAGGGTGGAGAATGA

Protein sequence

>MsG0480022879.01.T01

MDIRSILVPSVQELAKESLTNVPKRYILPDQDSVVLSNPTSSLPQVPVIDLAKLLSQDLNLKGHELEKLHSACKEWGFFQLVNHGVSSSLVENMKRGAKTLFELSMEEKKKLWQKEGDLEGFALFKCRENLEIYSTELEKLTIKMLELMANALAINPKEITEVFNIGTQAIRVNYYPPCPQPERVIGLKSHSDINGLTILLQISDIEGLQIKKDGQWIPIKPMPNAFIINIGDILEIITNGIYPSIEHRAIVNSEKERISLAAFYAPNMQAMLAPAPSLVTQERPAQYRRISVVDHFNGYFSQELRGKSYLNEMKITKGGE*