AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022932.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022932.01.T01 MTR_8g080180 93.817 372 7 2 1 367 1 361 0.0 718
MsG0480022932.01.T01 MTR_8g079482 30.871 379 185 10 47 355 58 429 1.34e-37 140
MsG0480022932.01.T01 MTR_8g079482 30.871 379 185 10 47 355 148 519 3.97e-37 140
MsG0480022932.01.T01 MTR_1g052560 42.593 108 62 0 251 358 165 272 1.10e-15 76.6
MsG0480022932.01.T01 MTR_7g091990 32.474 194 100 4 165 358 116 278 2.13e-15 75.9
MsG0480022932.01.T01 MTR_1g019280 38.235 102 63 0 253 354 509 610 5.89e-15 76.6
MsG0480022932.01.T01 MTR_8g023130 29.787 188 116 4 157 329 237 423 5.99e-15 76.3
MsG0480022932.01.T01 MTR_1g019280 38.235 102 63 0 253 354 447 548 6.38e-15 76.3
MsG0480022932.01.T01 MTR_1g100647 35.577 104 67 0 257 360 106 209 8.04e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022932.01.T01 AT1G67590 53.736 348 125 8 30 367 24 345 4.58e-113 333
MsG0480022932.01.T01 AT1G67590 48.387 279 108 8 30 298 24 276 8.95e-70 220
MsG0480022932.01.T01 AT2G02170 36.088 363 175 9 54 366 5 360 1.47e-44 157
MsG0480022932.01.T01 AT2G02170 36.088 363 175 9 54 366 5 360 1.47e-44 157
MsG0480022932.01.T01 AT2G02170 36.538 364 172 10 54 366 130 485 1.62e-43 157
MsG0480022932.01.T01 AT2G02170 36.538 364 172 10 54 366 130 485 1.62e-43 157
MsG0480022932.01.T01 AT2G02170 36.538 364 172 10 54 366 130 485 1.62e-43 157
MsG0480022932.01.T01 AT2G02170 36.538 364 172 10 54 366 130 485 1.62e-43 157
MsG0480022932.01.T01 AT1G30320 32.337 368 185 13 30 355 154 499 1.74e-39 147
MsG0480022932.01.T01 AT1G53860 30.479 292 169 10 60 329 54 333 1.37e-20 92.4
MsG0480022932.01.T01 AT1G53860 30.479 292 169 10 60 329 122 401 1.51e-20 92.8
MsG0480022932.01.T01 AT3G57540 39.496 119 71 1 244 362 178 295 4.09e-15 75.5
MsG0480022932.01.T01 AT2G41870 40.741 108 64 0 251 358 163 270 5.54e-15 74.7

Find 83 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATTACCTGGCCATATATT+TGG 0.179028 4:-78729545 None:intergenic
TACATTATGGCACTGCTATT+TGG 0.189520 4:-78728815 None:intergenic
GAATCATCATCGAATAAATA+TGG 0.218270 4:+78728898 MsG0480022932.01.T01:CDS
TCATCTGCATTTGAGTTTCT+TGG 0.225826 4:-78729010 None:intergenic
ATATTTATGCTACTTCTAAA+TGG 0.257290 4:+78731338 MsG0480022932.01.T01:three_prime_UTR
GTGAAACTCTGAATACATTA+TGG 0.286426 4:-78728828 None:intergenic
TGAAATGGAAATGAAGAAAA+TGG 0.303338 4:+78730023 MsG0480022932.01.T01:CDS
TGCGCGTCGTCCCACTTAGA+AGG 0.315412 4:-78728932 None:intergenic
GTAGGAGTTGTTGCTCTAAT+AGG 0.338684 4:-78729301 None:intergenic
GAATCATGGTGGAATTGAAT+TGG 0.340138 4:-78728009 None:intergenic
TTAAGCTGCCTTCCATTTGC+TGG 0.342268 4:+78731244 MsG0480022932.01.T01:CDS
TCAAAATGGTTTGTTGCAAA+TGG 0.364627 4:+78729369 MsG0480022932.01.T01:CDS
AGCAGAACGTGCCAAATATA+TGG 0.368956 4:+78729534 MsG0480022932.01.T01:CDS
TAGAAGTAGCATAAATATAA+TGG 0.374198 4:-78731333 None:intergenic
ACTCCAATGAGGAATCAAAA+TGG 0.385964 4:+78729355 MsG0480022932.01.T01:CDS
TCAGATAAGAAAAGCTGAAA+TGG 0.389501 4:+78730008 MsG0480022932.01.T01:CDS
GAGAATGAATAATAATAGTA+AGG 0.396414 4:+78729171 MsG0480022932.01.T01:CDS
AGAAAAGTTAACAAATAAAC+TGG 0.396943 4:+78731086 MsG0480022932.01.T01:CDS
GAAGGCTCTTGACTTGCAAT+AGG 0.403016 4:-78729265 None:intergenic
GTTGAAGTTCCTGAATGAAT+TGG 0.406805 4:-78729334 None:intergenic
ACTAGAAGTCCAATTCATTC+AGG 0.407197 4:+78729325 MsG0480022932.01.T01:CDS
TGGCTTGTTGGTTTATCAAA+AGG 0.409582 4:+78728959 MsG0480022932.01.T01:CDS
GAAGCTGCGTCGGGTAATAA+TGG 0.416106 4:+78729391 MsG0480022932.01.T01:CDS
GAAATATAACAGTAGTACTA+TGG 0.421847 4:+78727926 MsG0480022932.01.T01:five_prime_UTR
CAACATATAAATTAAACATA+TGG 0.427822 4:-78731281 None:intergenic
AATGTGACGATGAATCGATT+TGG 0.430490 4:+78729149 MsG0480022932.01.T01:CDS
GGTGCCATGTGGGTTGGAAT+TGG 0.442278 4:-78728076 None:intergenic
TGGTACTTTCTACCAGCAAA+TGG 0.460009 4:-78731256 None:intergenic
GTAGACTATATAAGAAGGAC+AGG 0.464307 4:+78731192 MsG0480022932.01.T01:CDS
TTGCAAATGGAAGCTGCGTC+GGG 0.468756 4:+78729382 MsG0480022932.01.T01:CDS
TTCTTCTCAACCATCTTACA+AGG 0.470028 4:-78729448 None:intergenic
TGAATTGGACTTCTAGTAGT+AGG 0.474528 4:-78729319 None:intergenic
AACTTTGAGAATGAAATTTG+TGG 0.475527 4:+78728745 MsG0480022932.01.T01:exon
AATCATCATCGAATAAATAT+GGG 0.479819 4:+78728899 MsG0480022932.01.T01:CDS
AGAATCTAGAGCAATGGCTT+GGG 0.482143 4:+78729507 MsG0480022932.01.T01:CDS
TATAATGGACTAGATTCAAA+AGG 0.492599 4:-78731318 None:intergenic
GTTAGCAAACAAAGGAATCA+TGG 0.498073 4:-78728023 None:intergenic
GAAAACCAACACCTTCTAAG+TGG 0.499969 4:+78728921 MsG0480022932.01.T01:CDS
TAGAATCTAGAGCAATGGCT+TGG 0.501769 4:+78729506 MsG0480022932.01.T01:CDS
GAGGAAGAACACGACGATGA+TGG 0.501892 4:+78729064 MsG0480022932.01.T01:CDS
AGCTGAATGACAATGCTTTG+AGG 0.503470 4:+78731157 MsG0480022932.01.T01:CDS
ATAGGTGTAGCAGTTCTTGA+AGG 0.505348 4:-78729283 None:intergenic
GTTGCAAATGGAAGCTGCGT+CGG 0.510774 4:+78729381 MsG0480022932.01.T01:CDS
ATGAAATTTGTGGAAGACAA+AGG 0.511994 4:+78728755 MsG0480022932.01.T01:CDS
TACTACTAAGCAAGTAATGC+AGG 0.515915 4:-78728097 None:intergenic
CGAATCAAGCCCTTGTAAGA+TGG 0.524282 4:+78729438 MsG0480022932.01.T01:CDS
TGCTTTGAGGACTACTGAAA+GGG 0.527867 4:+78731170 MsG0480022932.01.T01:CDS
AGAAATGAAGATTCAAGCCT+GGG 0.529083 4:+78729978 MsG0480022932.01.T01:CDS
TATAAATTAAACATATGGAT+TGG 0.530020 4:-78731276 None:intergenic
TCGTCCCACTTAGAAGGTGT+TGG 0.535229 4:-78728926 None:intergenic
TGAAGCAGAAGAGTCTAAGA+AGG 0.536028 4:+78729123 MsG0480022932.01.T01:CDS
AACGTGCCAAATATATGGCC+AGG 0.543371 4:+78729539 MsG0480022932.01.T01:CDS
AAGAAATGAAGATTCAAGCC+TGG 0.546327 4:+78729977 MsG0480022932.01.T01:CDS
CTTGTTGGTTTATCAAAAGG+TGG 0.547651 4:+78728962 MsG0480022932.01.T01:CDS
AAAGTGAAAGAGAATGAAGA+AGG 0.557122 4:-78731222 None:intergenic
GAGAATAGCTGAAGAGAAGA+GGG 0.566132 4:+78731119 MsG0480022932.01.T01:CDS
TAGAGTGAAGATATGAAATG+TGG 0.566919 4:+78728050 MsG0480022932.01.T01:five_prime_UTR
GGAGAATAGCTGAAGAGAAG+AGG 0.570917 4:+78731118 MsG0480022932.01.T01:CDS
AAAGGATGCTATAGTAACAA+TGG 0.573607 4:+78728773 MsG0480022932.01.T01:CDS
TCCTCTAGAATCTAGAGCAA+TGG 0.574161 4:+78729501 MsG0480022932.01.T01:CDS
ATGCTTTGAGGACTACTGAA+AGG 0.576263 4:+78731169 MsG0480022932.01.T01:CDS
AAAGGGTAGACTATATAAGA+AGG 0.578225 4:+78731187 MsG0480022932.01.T01:CDS
AGCTGAAAGAATGAAAGCTA+CGG 0.579161 4:+78731059 MsG0480022932.01.T01:CDS
TAAAACATATTGATTGAACA+TGG 0.587381 4:-78729219 None:intergenic
CAAGTAATGCAGGTGCCATG+TGG 0.594738 4:-78728087 None:intergenic
CAAATAAACTGGCTGCAACA+AGG 0.597519 4:+78731097 MsG0480022932.01.T01:CDS
AATGCAGGTGCCATGTGGGT+TGG 0.602262 4:-78728082 None:intergenic
ACTTTCTACCAGCAAATGGA+AGG 0.604910 4:-78731252 None:intergenic
TCTATAGAGTTAGCAAACAA+AGG 0.606393 4:-78728031 None:intergenic
AAAACCAACACCTTCTAAGT+GGG 0.608687 4:+78728922 MsG0480022932.01.T01:CDS
CAGAGACAAGTCCACTAGGT+AGG 0.612886 4:-78727969 None:intergenic
GTGTCAGAGACAAGTCCACT+AGG 0.617456 4:-78727973 None:intergenic
GCCATTGCTCTAGATTCTAG+AGG 0.622501 4:-78729502 None:intergenic
TCTTCTCAACCATCTTACAA+GGG 0.638564 4:-78729447 None:intergenic
GATTCCAATTCCAACCCACA+TGG 0.642127 4:+78728072 MsG0480022932.01.T01:five_prime_UTR
CAGGAACTTCAACTCCAATG+AGG 0.658899 4:+78729344 MsG0480022932.01.T01:CDS
AATGGAAATGAAGAAAATGG+AGG 0.667566 4:+78730026 MsG0480022932.01.T01:CDS
GGCAGCACAACTAGACAATG+TGG 0.670608 4:+78729412 MsG0480022932.01.T01:CDS
ATGTATTCAGAGTTTCACAA+AGG 0.675523 4:+78728833 MsG0480022932.01.T01:CDS
TAGTTGTTGTCATGAAGTCG+CGG 0.682281 4:-78729090 None:intergenic
AAGTAATGCAGGTGCCATGT+GGG 0.695838 4:-78728086 None:intergenic
AGCAAACAAAGGAATCATGG+TGG 0.717673 4:-78728020 None:intergenic
TAGACTATATAAGAAGGACA+GGG 0.724617 4:+78731193 MsG0480022932.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTATATTTTAATATTGAA+TGG + Chr4:78728264-78728283 MsG0480022932.01.T01:intron 10.0%
!!! TTGTTAAAACTTTATTTTAT+AGG + Chr4:78729944-78729963 MsG0480022932.01.T01:intron 10.0%
!! AAAACAGTACTATTTATATT+TGG + Chr4:78730434-78730453 MsG0480022932.01.T01:intron 15.0%
!! AGTAACAAAATCTTTAATTA+AGG - Chr4:78729842-78729861 None:intergenic 15.0%
!! CAACATATAAATTAAACATA+TGG - Chr4:78731284-78731303 None:intergenic 15.0%
!! TATAAATTAAACATATGGAT+TGG - Chr4:78731279-78731298 None:intergenic 15.0%
!! TTAAAACTTCAACAAAATTA+CGG + Chr4:78730170-78730189 MsG0480022932.01.T01:intron 15.0%
!!! TATGTATTTTTTTTTTGTCT+GGG - Chr4:78728595-78728614 None:intergenic 15.0%
!! AAAACAGATGTATATATCAA+AGG - Chr4:78728459-78728478 None:intergenic 20.0%
!! AAACAACAATGAAAATTTCA+GGG - Chr4:78728698-78728717 None:intergenic 20.0%
!! AAATTACTTCAATCTAGATA+AGG + Chr4:78728563-78728582 MsG0480022932.01.T01:intron 20.0%
!! AACAGTTTACATTTATTCAA+CGG - Chr4:78729912-78729931 None:intergenic 20.0%
!! AATCATCATCGAATAAATAT+GGG + Chr4:78728899-78728918 MsG0480022932.01.T01:CDS 20.0%
!! AGAAAAGTTAACAAATAAAC+TGG + Chr4:78731086-78731105 MsG0480022932.01.T01:CDS 20.0%
!! CATACTCAAATAACTTTATT+AGG - Chr4:78730902-78730921 None:intergenic 20.0%
!! GAGAATGAATAATAATAGTA+AGG + Chr4:78729171-78729190 MsG0480022932.01.T01:CDS 20.0%
!! TAAGAAAATATACTATGCAT+GGG - Chr4:78728236-78728255 None:intergenic 20.0%
!! TAAGTATGTTTGTTTATCTA+AGG + Chr4:78730938-78730957 MsG0480022932.01.T01:intron 20.0%
!! TAGAAGTAGCATAAATATAA+TGG - Chr4:78731336-78731355 None:intergenic 20.0%
!! TTAAGAAAATATACTATGCA+TGG - Chr4:78728237-78728256 None:intergenic 20.0%
!!! ATATTTATGCTACTTCTAAA+TGG + Chr4:78731338-78731357 MsG0480022932.01.T01:three_prime_UTR 20.0%
!!! CTATGTATTTTTTTTTTGTC+TGG - Chr4:78728596-78728615 None:intergenic 20.0%
!!! GATTTGATTTAATTGTTACA+AGG + Chr4:78731032-78731051 MsG0480022932.01.T01:intron 20.0%
!!! GTTTTCAAGTATTAATAAGA+AGG + Chr4:78728330-78728349 MsG0480022932.01.T01:intron 20.0%
!!! TAAAACATATTGATTGAACA+TGG - Chr4:78729222-78729241 None:intergenic 20.0%
!!! TTGTTAGTTTATTTCATGTT+AGG + Chr4:78729861-78729880 MsG0480022932.01.T01:intron 20.0%
! ATTTACATTACATGCAAGTA+AGG + Chr4:78728525-78728544 MsG0480022932.01.T01:intron 25.0%
! CATAGAATAACAATTTACCT+TGG - Chr4:78728155-78728174 None:intergenic 25.0%
! GAAACAACAATGAAAATTTC+AGG - Chr4:78728699-78728718 None:intergenic 25.0%
! GAAATATAACAGTAGTACTA+TGG + Chr4:78727926-78727945 MsG0480022932.01.T01:five_prime_UTR 25.0%
! GAATCATCATCGAATAAATA+TGG + Chr4:78728898-78728917 MsG0480022932.01.T01:CDS 25.0%
! TATCATTGGCAATAATTGAA+GGG - Chr4:78730252-78730271 None:intergenic 25.0%
! TCAAAGCTCTATCAAATAAA+TGG + Chr4:78731010-78731029 MsG0480022932.01.T01:intron 25.0%
! TGAAATGGAAATGAAGAAAA+TGG + Chr4:78730023-78730042 MsG0480022932.01.T01:CDS 25.0%
!! AACTTTGAGAATGAAATTTG+TGG + Chr4:78728745-78728764 MsG0480022932.01.T01:exon 25.0%
!!! ATCAATATGTTTTAGAGACA+TGG + Chr4:78729228-78729247 MsG0480022932.01.T01:CDS 25.0%
!!! TATAATGGACTAGATTCAAA+AGG - Chr4:78731321-78731340 None:intergenic 25.0%
!!! TCAATATGTTTTAGAGACAT+GGG + Chr4:78729229-78729248 MsG0480022932.01.T01:CDS 25.0%
AAAAAGCTCATCAATTTCCA+AGG + Chr4:78728135-78728154 MsG0480022932.01.T01:five_prime_UTR 30.0%
AAAGGATGCTATAGTAACAA+TGG + Chr4:78728773-78728792 MsG0480022932.01.T01:CDS 30.0%
AAAGGGTAGACTATATAAGA+AGG + Chr4:78731187-78731206 MsG0480022932.01.T01:CDS 30.0%
AAAGTGAAAGAGAATGAAGA+AGG - Chr4:78731225-78731244 None:intergenic 30.0%
AATGGAAATGAAGAAAATGG+AGG + Chr4:78730026-78730045 MsG0480022932.01.T01:CDS 30.0%
ACAGATGTATATATCAAAGG+AGG - Chr4:78728456-78728475 None:intergenic 30.0%
ATGAAATTTGTGGAAGACAA+AGG + Chr4:78728755-78728774 MsG0480022932.01.T01:CDS 30.0%
ATGTATATATCAAAGGAGGA+AGG - Chr4:78728452-78728471 None:intergenic 30.0%
ATGTATTCAGAGTTTCACAA+AGG + Chr4:78728833-78728852 MsG0480022932.01.T01:CDS 30.0%
CATAGTGACTTTGAATAGAT+AGG + Chr4:78728650-78728669 MsG0480022932.01.T01:intron 30.0%
CTATCTATTCTTGTCCTAAA+TGG + Chr4:78728190-78728209 MsG0480022932.01.T01:intron 30.0%
CTTGATACTTAGTGTATGTT+TGG + Chr4:78729569-78729588 MsG0480022932.01.T01:intron 30.0%
GAATCAGTAATGTACTGTTA+TGG - Chr4:78730531-78730550 None:intergenic 30.0%
GTATCATTGGCAATAATTGA+AGG - Chr4:78730253-78730272 None:intergenic 30.0%
GTGAAACTCTGAATACATTA+TGG - Chr4:78728831-78728850 None:intergenic 30.0%
TAGACTATATAAGAAGGACA+GGG + Chr4:78731193-78731212 MsG0480022932.01.T01:CDS 30.0%
TAGAGTGAAGATATGAAATG+TGG + Chr4:78728050-78728069 MsG0480022932.01.T01:five_prime_UTR 30.0%
TATTATTGATGTTAGCGAGT+CGG - Chr4:78730725-78730744 None:intergenic 30.0%
TCAGATAAGAAAAGCTGAAA+TGG + Chr4:78730008-78730027 MsG0480022932.01.T01:CDS 30.0%
TCTATAGAGTTAGCAAACAA+AGG - Chr4:78728034-78728053 None:intergenic 30.0%
TTATGATGACAGAGTTCATA+AGG + Chr4:78730315-78730334 MsG0480022932.01.T01:intron 30.0%
! AATGATACCAACCTTTTCTA+AGG + Chr4:78730265-78730284 MsG0480022932.01.T01:intron 30.0%
! CAAAGTTTTGCTTGTAACTA+AGG - Chr4:78728732-78728751 None:intergenic 30.0%
! CATTTAGGACAAGAATAGAT+AGG - Chr4:78728192-78728211 None:intergenic 30.0%
! TTTTGCTTGTAACTAAGGAA+AGG - Chr4:78728727-78728746 None:intergenic 30.0%
! TTTTGTGCAAGTAGAAATGA+AGG + Chr4:78730834-78730853 MsG0480022932.01.T01:intron 30.0%
!! TAAGTATCAAGTTGATTACC+TGG - Chr4:78729560-78729579 None:intergenic 30.0%
!! TCAAAATGGTTTGTTGCAAA+TGG + Chr4:78729369-78729388 MsG0480022932.01.T01:CDS 30.0%
!! TTAGAAAAGGTTGGTATCAT+TGG - Chr4:78730266-78730285 None:intergenic 30.0%
!!! AAACCATTTTGATTCCTCAT+TGG - Chr4:78729361-78729380 None:intergenic 30.0%
AAAACCAACACCTTCTAAGT+GGG + Chr4:78728922-78728941 MsG0480022932.01.T01:CDS 35.0%
AAGAAATGAAGATTCAAGCC+TGG + Chr4:78729977-78729996 MsG0480022932.01.T01:CDS 35.0%
AATGTGACGATGAATCGATT+TGG + Chr4:78729149-78729168 MsG0480022932.01.T01:CDS 35.0%
AATTCACATTCTCGACAGTT+TGG + Chr4:78730087-78730106 MsG0480022932.01.T01:intron 35.0%
ACATGGATGTTTGGAAGAAA+TGG - Chr4:78730204-78730223 None:intergenic 35.0%
ACTAGAAGTCCAATTCATTC+AGG + Chr4:78729325-78729344 MsG0480022932.01.T01:CDS 35.0%
ACTCCAATGAGGAATCAAAA+TGG + Chr4:78729355-78729374 MsG0480022932.01.T01:CDS 35.0%
AGAAATGAAGATTCAAGCCT+GGG + Chr4:78729978-78729997 MsG0480022932.01.T01:CDS 35.0%
AGCTGAAAGAATGAAAGCTA+CGG + Chr4:78731059-78731078 MsG0480022932.01.T01:CDS 35.0%
ATCTATGCTAGTACGATTGT+AGG - Chr4:78730671-78730690 None:intergenic 35.0%
ATTAGGCTAGATTCTCACAT+AGG - Chr4:78730576-78730595 None:intergenic 35.0%
ATTTCTTCCAAACATCCATG+TGG + Chr4:78730203-78730222 MsG0480022932.01.T01:intron 35.0%
CTGTTATGGAGCTATCAAAT+AGG - Chr4:78730517-78730536 None:intergenic 35.0%
GACTTTGAATAGATAGGATC+TGG + Chr4:78728656-78728675 MsG0480022932.01.T01:intron 35.0%
GTAGACTATATAAGAAGGAC+AGG + Chr4:78731192-78731211 MsG0480022932.01.T01:CDS 35.0%
GTTAGCAAACAAAGGAATCA+TGG - Chr4:78728026-78728045 None:intergenic 35.0%
GTTGAAGTTCCTGAATGAAT+TGG - Chr4:78729337-78729356 None:intergenic 35.0%
GTTGTGACTGCATATTTCAT+TGG - Chr4:78730487-78730506 None:intergenic 35.0%
TACATTATGGCACTGCTATT+TGG - Chr4:78728818-78728837 None:intergenic 35.0%
TACTACTAAGCAAGTAATGC+AGG - Chr4:78728100-78728119 None:intergenic 35.0%
TACTCATTAAATTGAGCACC+TGG - Chr4:78730631-78730650 None:intergenic 35.0%
TATGCATGGGTTCACAAAAA+GGG - Chr4:78728223-78728242 None:intergenic 35.0%
TCTTCTCAACCATCTTACAA+GGG - Chr4:78729450-78729469 None:intergenic 35.0%
TGAATTGGACTTCTAGTAGT+AGG - Chr4:78729322-78729341 None:intergenic 35.0%
TGATTACCTGGCCATATATT+TGG - Chr4:78729548-78729567 None:intergenic 35.0%
TTCTTCTCAACCATCTTACA+AGG - Chr4:78729451-78729470 None:intergenic 35.0%
! ACTAGTATTTTCCTACCTAG+TGG + Chr4:78727958-78727977 MsG0480022932.01.T01:five_prime_UTR 35.0%
! GAATCATGGTGGAATTGAAT+TGG - Chr4:78728012-78728031 None:intergenic 35.0%
! TCATCTGCATTTGAGTTTCT+TGG - Chr4:78729013-78729032 None:intergenic 35.0%
! TCTTATCTGATGATTTTCCC+AGG - Chr4:78729998-78730017 None:intergenic 35.0%
! TGGATTGACTTATTTGCTGA+TGG + Chr4:78729589-78729608 MsG0480022932.01.T01:intron 35.0%
! TGTTTTGTATGCAGTACGTT+GGG - Chr4:78730420-78730439 None:intergenic 35.0%
!! CTTGTTGGTTTATCAAAAGG+TGG + Chr4:78728962-78728981 MsG0480022932.01.T01:CDS 35.0%
!! TCCTGAAAAGTTCTAAGACA+TGG + Chr4:78730383-78730402 MsG0480022932.01.T01:intron 35.0%
!! TGGCTTGTTGGTTTATCAAA+AGG + Chr4:78728959-78728978 MsG0480022932.01.T01:CDS 35.0%
!!! TTCTTGGTTTGCTTTTTGAC+TGG - Chr4:78728997-78729016 None:intergenic 35.0%
AAAGCTACCACATGGATGTT+TGG - Chr4:78730213-78730232 None:intergenic 40.0%
ACCATCCAATGCCTTAGAAA+AGG - Chr4:78730279-78730298 None:intergenic 40.0%
ACTTTCTACCAGCAAATGGA+AGG - Chr4:78731255-78731274 None:intergenic 40.0%
AGCAAACAAAGGAATCATGG+TGG - Chr4:78728023-78728042 None:intergenic 40.0%
AGCAGAACGTGCCAAATATA+TGG + Chr4:78729534-78729553 MsG0480022932.01.T01:CDS 40.0%
AGCTGAATGACAATGCTTTG+AGG + Chr4:78731157-78731176 MsG0480022932.01.T01:CDS 40.0%
ATAGGTGTAGCAGTTCTTGA+AGG - Chr4:78729286-78729305 None:intergenic 40.0%
CAAATAAACTGGCTGCAACA+AGG + Chr4:78731097-78731116 MsG0480022932.01.T01:CDS 40.0%
CTATGCATGGGTTCACAAAA+AGG - Chr4:78728224-78728243 None:intergenic 40.0%
CTGCAACTCTCAGAATTTCA+TGG + Chr4:78728308-78728327 MsG0480022932.01.T01:intron 40.0%
GAAAACCAACACCTTCTAAG+TGG + Chr4:78728921-78728940 MsG0480022932.01.T01:CDS 40.0%
GAGAATAGCTGAAGAGAAGA+GGG + Chr4:78731119-78731138 MsG0480022932.01.T01:CDS 40.0%
GTAGGAGTTGTTGCTCTAAT+AGG - Chr4:78729304-78729323 None:intergenic 40.0%
TAGTTGTTGTCATGAAGTCG+CGG - Chr4:78729093-78729112 None:intergenic 40.0%
TCCAATGCCTTAGAAAAGGT+TGG - Chr4:78730275-78730294 None:intergenic 40.0%
TGAAGCAGAAGAGTCTAAGA+AGG + Chr4:78729123-78729142 MsG0480022932.01.T01:CDS 40.0%
TGGATGTTTGGAAGAAATGG+TGG - Chr4:78730201-78730220 None:intergenic 40.0%
TGGTACTTTCTACCAGCAAA+TGG - Chr4:78731259-78731278 None:intergenic 40.0%
TTCTCGACAGTTTGGATTCA+CGG + Chr4:78730095-78730114 MsG0480022932.01.T01:intron 40.0%
! AGAATCTAGAGCAATGGCTT+GGG + Chr4:78729507-78729526 MsG0480022932.01.T01:CDS 40.0%
! CTGTTTTGTATGCAGTACGT+TGG - Chr4:78730421-78730440 None:intergenic 40.0%
! GCACGCACACTTTTCAAATT+AGG - Chr4:78730787-78730806 None:intergenic 40.0%
! GCCATGTCTTAGAACTTTTC+AGG - Chr4:78730387-78730406 None:intergenic 40.0%
! TAGAATCTAGAGCAATGGCT+TGG + Chr4:78729506-78729525 MsG0480022932.01.T01:CDS 40.0%
! TCGTGTTCTTCCTCTTTTTG+TGG - Chr4:78729058-78729077 None:intergenic 40.0%
!! ATGCTTTGAGGACTACTGAA+AGG + Chr4:78731169-78731188 MsG0480022932.01.T01:CDS 40.0%
!! GAAAAGTTCTAAGACATGGC+AGG + Chr4:78730387-78730406 MsG0480022932.01.T01:intron 40.0%
!! TCCTCTAGAATCTAGAGCAA+TGG + Chr4:78729501-78729520 MsG0480022932.01.T01:CDS 40.0%
!! TGCTTTGAGGACTACTGAAA+GGG + Chr4:78731170-78731189 MsG0480022932.01.T01:CDS 40.0%
!!! ACCAACCTTTTCTAAGGCAT+TGG + Chr4:78730271-78730290 MsG0480022932.01.T01:intron 40.0%
!!! ACCTTTTCTAAGGCATTGGA+TGG + Chr4:78730275-78730294 MsG0480022932.01.T01:intron 40.0%
!!! TTTTCTAAGGCATTGGATGG+TGG + Chr4:78730278-78730297 MsG0480022932.01.T01:intron 40.0%
AACGTGCCAAATATATGGCC+AGG + Chr4:78729539-78729558 MsG0480022932.01.T01:CDS 45.0%
ATGGGTTCACAAAAAGGGAG+GGG - Chr4:78728218-78728237 None:intergenic 45.0%
CAGGAACTTCAACTCCAATG+AGG + Chr4:78729344-78729363 MsG0480022932.01.T01:CDS 45.0%
CATGGGTTCACAAAAAGGGA+GGG - Chr4:78728219-78728238 None:intergenic 45.0%
CGAATCAAGCCCTTGTAAGA+TGG + Chr4:78729438-78729457 MsG0480022932.01.T01:CDS 45.0%
GAAACACCTGCGATACTCTT+TGG + Chr4:78729611-78729630 MsG0480022932.01.T01:intron 45.0%
GATTCCAATTCCAACCCACA+TGG + Chr4:78728072-78728091 MsG0480022932.01.T01:five_prime_UTR 45.0%
GGAGAATAGCTGAAGAGAAG+AGG + Chr4:78731118-78731137 MsG0480022932.01.T01:CDS 45.0%
TGTATGCAGTACGTTGGGAA+AGG - Chr4:78730415-78730434 None:intergenic 45.0%
TTAAGCTGCCTTCCATTTGC+TGG + Chr4:78731244-78731263 MsG0480022932.01.T01:CDS 45.0%
! AAGTAATGCAGGTGCCATGT+GGG - Chr4:78728089-78728108 None:intergenic 45.0%
! CATCCATGTGGTAGCTTTTG+TGG + Chr4:78730215-78730234 MsG0480022932.01.T01:intron 45.0%
! CATGCGTTGCTTTCATGAGT+TGG + Chr4:78730603-78730622 MsG0480022932.01.T01:intron 45.0%
! GCAACGCATGTCTGTTGATT+AGG - Chr4:78730593-78730612 None:intergenic 45.0%
! GCCATTGCTCTAGATTCTAG+AGG - Chr4:78729505-78729524 None:intergenic 45.0%
! TCTTCCTCTTTTTGTGGCAC+AGG - Chr4:78729052-78729071 None:intergenic 45.0%
!! GAAGGCTCTTGACTTGCAAT+AGG - Chr4:78729268-78729287 None:intergenic 45.0%
!! GCATAGATGAAGCACTGACA+CGG + Chr4:78730683-78730702 MsG0480022932.01.T01:intron 45.0%
!!! TTTTTTTTTATGTAAATATA+TGG + Chr4:78729763-78729782 MsG0480022932.01.T01:intron 5.0%
AGCTCCTGTGCCACAAAAAG+AGG + Chr4:78729045-78729064 MsG0480022932.01.T01:CDS 50.0%
CAGAGACAAGTCCACTAGGT+AGG - Chr4:78727972-78727991 None:intergenic 50.0%
GAAGCTGCGTCGGGTAATAA+TGG + Chr4:78729391-78729410 MsG0480022932.01.T01:CDS 50.0%
GAGGAAGAACACGACGATGA+TGG + Chr4:78729064-78729083 MsG0480022932.01.T01:CDS 50.0%
GCACCACAAAAGCTACCACA+TGG - Chr4:78730221-78730240 None:intergenic 50.0%
GCATGGGTTCACAAAAAGGG+AGG - Chr4:78728220-78728239 None:intergenic 50.0%
GGCAGCACAACTAGACAATG+TGG + Chr4:78729412-78729431 MsG0480022932.01.T01:CDS 50.0%
GTGTCAGAGACAAGTCCACT+AGG - Chr4:78727976-78727995 None:intergenic 50.0%
GTTGCAAATGGAAGCTGCGT+CGG + Chr4:78729381-78729400 MsG0480022932.01.T01:CDS 50.0%
TGGGTTCACAAAAAGGGAGG+GGG - Chr4:78728217-78728236 None:intergenic 50.0%
TTGCAAATGGAAGCTGCGTC+GGG + Chr4:78729382-78729401 MsG0480022932.01.T01:CDS 50.0%
! CAAGTAATGCAGGTGCCATG+TGG - Chr4:78728090-78728109 None:intergenic 50.0%
! GACGCGCAAAAATGGCTTGT+TGG + Chr4:78728947-78728966 MsG0480022932.01.T01:CDS 50.0%
! TAGCGAGTCGGTGTTTGTGT+CGG - Chr4:78730713-78730732 None:intergenic 50.0%
! TCGTCCCACTTAGAAGGTGT+TGG - Chr4:78728929-78728948 None:intergenic 50.0%
! TGCTTTCATGAGTTGGCACC+AGG + Chr4:78730610-78730629 MsG0480022932.01.T01:intron 50.0%
!! GCTCTTCCAAAGAGTATCGC+AGG - Chr4:78729620-78729639 None:intergenic 50.0%
AAAAGGGAGGGGGGCCATTT+AGG - Chr4:78728207-78728226 None:intergenic 55.0%
AGTGGGACGACGCGCAAAAA+TGG + Chr4:78728939-78728958 MsG0480022932.01.T01:CDS 55.0%
GGGTTCACAAAAAGGGAGGG+GGG - Chr4:78728216-78728235 None:intergenic 55.0%
! AATGCAGGTGCCATGTGGGT+TGG - Chr4:78728085-78728104 None:intergenic 55.0%
! AGCGAGTCGGTGTTTGTGTC+GGG - Chr4:78730712-78730731 None:intergenic 55.0%
!! GGTGCCATGTGGGTTGGAAT+TGG - Chr4:78728079-78728098 None:intergenic 55.0%
TGCGCGTCGTCCCACTTAGA+AGG - Chr4:78728935-78728954 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 78727925 78731379 78727925 ID=MsG0480022932.01;Name=MsG0480022932.01
Chr4 mRNA 78727925 78731379 78727925 ID=MsG0480022932.01.T01;Parent=MsG0480022932.01;Name=MsG0480022932.01.T01;_AED=0.29;_eAED=0.29;_QI=252|1|1|1|0.66|0.5|4|110|367
Chr4 exon 78727925 78728156 78727925 ID=MsG0480022932.01.T01:exon:11597;Parent=MsG0480022932.01.T01
Chr4 exon 78728735 78729560 78728735 ID=MsG0480022932.01.T01:exon:11598;Parent=MsG0480022932.01.T01
Chr4 exon 78729966 78730047 78729966 ID=MsG0480022932.01.T01:exon:11599;Parent=MsG0480022932.01.T01
Chr4 exon 78731054 78731379 78731054 ID=MsG0480022932.01.T01:exon:11600;Parent=MsG0480022932.01.T01
Chr4 five_prime_UTR 78727925 78728156 78727925 ID=MsG0480022932.01.T01:five_prime_utr;Parent=MsG0480022932.01.T01
Chr4 five_prime_UTR 78728735 78728754 78728735 ID=MsG0480022932.01.T01:five_prime_utr;Parent=MsG0480022932.01.T01
Chr4 CDS 78728755 78729560 78728755 ID=MsG0480022932.01.T01:cds;Parent=MsG0480022932.01.T01
Chr4 CDS 78729966 78730047 78729966 ID=MsG0480022932.01.T01:cds;Parent=MsG0480022932.01.T01
Chr4 CDS 78731054 78731269 78731054 ID=MsG0480022932.01.T01:cds;Parent=MsG0480022932.01.T01
Chr4 three_prime_UTR 78731270 78731379 78731270 ID=MsG0480022932.01.T01:three_prime_utr;Parent=MsG0480022932.01.T01
Gene Sequence

>MsG0480022932.01.T01

ATGAAATTTGTGGAAGACAAAGGATGCTATAGTAACAATGGATCAGCAGCACAAGATACTCCAAATAGCAGTGCCATAATGTATTCAGAGTTTCACAAAGGAAAAGATACTACTACTAGTACTACTGCTACTACTCGTGTTTCGAATCATCATCGAATAAATATGGGAAAACCAACACCTTCTAAGTGGGACGACGCGCAAAAATGGCTTGTTGGTTTATCAAAAGGTGGAGAGAAAAGCCAGTCAAAAAGCAAACCAAGAAACTCAAATGCAGATGATTTAAGATTGATAGCTCCTGTGCCACAAAAAGAGGAAGAACACGACGATGATGGATGCCGCGACTTCATGACAACAACTAGTAGTGAATATGAAGCAGAAGAGTCTAAGAAGGTAGAATGTGACGATGAATCGATTTGGAGAATGAATAATAATAGTAAGGTTTTAGCAGAAAACAAAAACACAATCCATGTTCAATCAATATGTTTTAGAGACATGGGAACTGAGATGACACCTATTGCAAGTCAAGAGCCTTCAAGAACTGCTACACCTATTAGAGCAACAACTCCTACTACTAGAAGTCCAATTCATTCAGGAACTTCAACTCCAATGAGGAATCAAAATGGTTTGTTGCAAATGGAAGCTGCGTCGGGTAATAATGGCAGCACAACTAGACAATGTGGAGACGAATCAAGCCCTTGTAAGATGGTTGAGAAGAACAAGATTGAAGATCAAGCTAGAAAGTTGAGTCCTCTAGAATCTAGAGCAATGGCTTGGGATGAAGCAGAACGTGCCAAATATATGGCCAGGTTTAAGCGTGAAGAAATGAAGATTCAAGCCTGGGAAAATCATCAGATAAGAAAAGCTGAAATGGAAATGAAGAAAATGGAGGTGAAAGCTGAAAGAATGAAAGCTACGGCACAAGAAAAGTTAACAAATAAACTGGCTGCAACAAGGAGAATAGCTGAAGAGAAGAGGGCTAATGCACAAGTGAAGCTGAATGACAATGCTTTGAGGACTACTGAAAGGGTAGACTATATAAGAAGGACAGGGCATGTGCCTTCTTCATTCTCTTTCACTTTTAAGCTGCCTTCCATTTGCTGGTAG
Protein sequence

>MsG0480022932.01.T01

MKFVEDKGCYSNNGSAAQDTPNSSAIMYSEFHKGKDTTTSTTATTRVSNHHRINMGKPTPSKWDDAQKWLVGLSKGGEKSQSKSKPRNSNADDLRLIAPVPQKEEEHDDDGCRDFMTTTSSEYEAEESKKVECDDESIWRMNNNSKVLAENKNTIHVQSICFRDMGTEMTPIASQEPSRTATPIRATTPTTRSPIHSGTSTPMRNQNGLLQMEAASGNNGSTTRQCGDESSPCKMVEKNKIEDQARKLSPLESRAMAWDEAERAKYMARFKREEMKIQAWENHQIRKAEMEMKKMEVKAERMKATAQEKLTNKLAATRRIAEEKRANAQVKLNDNALRTTERVDYIRRTGHVPSSFSFTFKLPSICW*