Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023120.01.T01 | AET04331.1 | 85.246 | 183 | 27 | 0 | 1 | 183 | 8 | 190 | 2.62E-108 | 318 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023120.01.T01 | G7L7A8 | 85.246 | 183 | 27 | 0 | 1 | 183 | 8 | 190 | 1.25e-108 | 318 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480023120.01 | MsG0480023121.01 | 0.803181 | 3.940976e-49 | 1.967383e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023120.01.T01 | MTR_8g087630 | 85.246 | 183 | 27 | 0 | 1 | 183 | 8 | 190 | 3.17e-112 | 318 |
| MsG0480023120.01.T01 | MTR_8g087590 | 79.213 | 178 | 32 | 3 | 7 | 181 | 79 | 254 | 7.40e-93 | 271 |
| MsG0480023120.01.T01 | MTR_8g087650 | 74.586 | 181 | 42 | 2 | 7 | 183 | 14 | 194 | 8.97e-92 | 266 |
| MsG0480023120.01.T01 | MTR_8g087610 | 71.892 | 185 | 45 | 2 | 6 | 183 | 24 | 208 | 9.83e-89 | 259 |
| MsG0480023120.01.T01 | MTR_8g087640 | 75.460 | 163 | 40 | 0 | 18 | 180 | 71 | 233 | 8.27e-85 | 250 |
| MsG0480023120.01.T01 | MTR_8g087640 | 75.460 | 163 | 40 | 0 | 18 | 180 | 115 | 277 | 1.32e-84 | 251 |
| MsG0480023120.01.T01 | MTR_8g087570 | 67.232 | 177 | 35 | 3 | 8 | 181 | 98 | 254 | 8.96e-73 | 220 |
| MsG0480023120.01.T01 | MTR_8g087570 | 67.232 | 177 | 35 | 3 | 8 | 181 | 60 | 216 | 1.40e-72 | 218 |
| MsG0480023120.01.T01 | MTR_8g087570 | 67.232 | 177 | 35 | 3 | 8 | 181 | 60 | 216 | 1.40e-72 | 218 |
| MsG0480023120.01.T01 | MTR_8g087600 | 71.774 | 124 | 33 | 1 | 44 | 167 | 8 | 129 | 5.04e-59 | 187 |
| MsG0480023120.01.T01 | MTR_8g087600 | 58.710 | 155 | 33 | 3 | 29 | 181 | 178 | 303 | 4.35e-49 | 161 |
| MsG0480023120.01.T01 | MTR_8g087555 | 68.707 | 147 | 18 | 1 | 37 | 183 | 4 | 122 | 1.35e-58 | 179 |
| MsG0480023120.01.T01 | MTR_3g013790 | 33.540 | 161 | 88 | 2 | 22 | 181 | 153 | 295 | 9.68e-21 | 89.4 |
| MsG0480023120.01.T01 | MTR_3g013790 | 33.540 | 161 | 88 | 2 | 22 | 181 | 153 | 295 | 1.22e-20 | 89.0 |
| MsG0480023120.01.T01 | MTR_2g072820 | 35.583 | 163 | 84 | 3 | 20 | 181 | 160 | 302 | 7.01e-20 | 86.7 |
| MsG0480023120.01.T01 | MTR_2g072030 | 33.333 | 189 | 97 | 6 | 1 | 184 | 143 | 307 | 5.14e-19 | 84.3 |
| MsG0480023120.01.T01 | MTR_2g072080 | 34.356 | 163 | 86 | 4 | 20 | 181 | 162 | 304 | 5.91e-18 | 81.3 |
| MsG0480023120.01.T01 | MTR_2g072150 | 30.978 | 184 | 101 | 5 | 3 | 181 | 142 | 304 | 1.08e-17 | 80.5 |
| MsG0480023120.01.T01 | MTR_2g072110 | 33.129 | 163 | 88 | 3 | 20 | 181 | 162 | 304 | 3.96e-17 | 79.0 |
| MsG0480023120.01.T01 | MTR_2g072110 | 33.129 | 163 | 88 | 3 | 20 | 181 | 162 | 304 | 4.20e-17 | 79.0 |
| MsG0480023120.01.T01 | MTR_1g069615 | 33.333 | 177 | 93 | 6 | 4 | 177 | 30 | 184 | 5.60e-17 | 75.1 |
| MsG0480023120.01.T01 | MTR_2g071820 | 33.553 | 152 | 80 | 3 | 20 | 170 | 162 | 293 | 8.59e-17 | 77.8 |
| MsG0480023120.01.T01 | MTR_2g072020 | 29.825 | 171 | 99 | 3 | 14 | 181 | 152 | 304 | 1.86e-16 | 77.0 |
| MsG0480023120.01.T01 | MTR_1g037150 | 34.161 | 161 | 84 | 4 | 18 | 177 | 148 | 287 | 4.40e-16 | 75.9 |
| MsG0480023120.01.T01 | MTR_1g036860 | 32.680 | 153 | 82 | 3 | 18 | 169 | 160 | 292 | 4.62e-16 | 75.5 |
| MsG0480023120.01.T01 | MTR_2g071890 | 33.553 | 152 | 80 | 3 | 20 | 170 | 162 | 293 | 5.87e-16 | 75.5 |
| MsG0480023120.01.T01 | MTR_0006s0240 | 33.540 | 161 | 85 | 4 | 18 | 177 | 161 | 300 | 7.19e-16 | 75.1 |
| MsG0480023120.01.T01 | MTR_0069s0040 | 33.333 | 153 | 81 | 3 | 18 | 169 | 160 | 292 | 1.06e-15 | 74.7 |
| MsG0480023120.01.T01 | MTR_1g041550 | 33.987 | 153 | 80 | 3 | 18 | 169 | 161 | 293 | 1.88e-15 | 73.9 |
| MsG0480023120.01.T01 | MTR_1g036750 | 33.824 | 136 | 71 | 2 | 35 | 169 | 144 | 261 | 3.00e-15 | 73.6 |
| MsG0480023120.01.T01 | MTR_1g051585 | 35.652 | 115 | 56 | 1 | 55 | 169 | 196 | 292 | 4.43e-15 | 72.8 |
| MsG0480023120.01.T01 | MTR_1g037010 | 33.824 | 136 | 71 | 2 | 35 | 169 | 176 | 293 | 8.97e-15 | 72.0 |
| MsG0480023120.01.T01 | MTR_1g037130 | 34.188 | 117 | 59 | 1 | 55 | 171 | 196 | 294 | 9.13e-15 | 72.0 |
| MsG0480023120.01.T01 | MTR_0006s0230 | 33.824 | 136 | 71 | 2 | 35 | 169 | 176 | 293 | 2.02e-14 | 71.2 |
| MsG0480023120.01.T01 | MTR_2g071900 | 33.594 | 128 | 67 | 2 | 54 | 181 | 195 | 304 | 2.07e-14 | 70.9 |
| MsG0480023120.01.T01 | MTR_2g071940 | 33.594 | 128 | 67 | 2 | 54 | 181 | 199 | 308 | 3.53e-14 | 70.1 |
| MsG0480023120.01.T01 | MTR_2g071870 | 33.594 | 128 | 67 | 2 | 54 | 181 | 199 | 308 | 5.85e-14 | 69.7 |
| MsG0480023120.01.T01 | MTR_2g072090 | 33.594 | 128 | 67 | 2 | 54 | 181 | 199 | 308 | 1.30e-13 | 68.6 |
| MsG0480023120.01.T01 | MTR_3g467640 | 28.387 | 155 | 91 | 3 | 28 | 181 | 7 | 142 | 2.19e-13 | 68.2 |
| MsG0480023120.01.T01 | MTR_0006s0140 | 33.913 | 115 | 58 | 1 | 55 | 169 | 196 | 292 | 3.17e-13 | 67.0 |
| MsG0480023120.01.T01 | MTR_2g071980 | 32.812 | 128 | 68 | 2 | 54 | 181 | 195 | 304 | 4.06e-13 | 67.4 |
| MsG0480023120.01.T01 | MTR_5g062770 | 29.050 | 179 | 105 | 4 | 6 | 181 | 124 | 283 | 4.20e-13 | 67.0 |
| MsG0480023120.01.T01 | MTR_2g071850 | 32.812 | 128 | 68 | 2 | 54 | 181 | 199 | 308 | 4.47e-13 | 67.0 |
| MsG0480023120.01.T01 | MTR_0006s0080 | 35.652 | 115 | 56 | 1 | 55 | 169 | 197 | 293 | 6.53e-13 | 66.6 |
| MsG0480023120.01.T01 | MTR_8g059345 | 35.652 | 115 | 56 | 1 | 55 | 169 | 196 | 292 | 8.13e-13 | 66.2 |
| MsG0480023120.01.T01 | MTR_1g044020 | 29.940 | 167 | 92 | 3 | 6 | 169 | 149 | 293 | 1.01e-12 | 66.2 |
| MsG0480023120.01.T01 | MTR_6g463150 | 31.293 | 147 | 80 | 3 | 18 | 163 | 160 | 286 | 1.37e-12 | 65.9 |
| MsG0480023120.01.T01 | MTR_7g026250 | 29.268 | 164 | 94 | 4 | 19 | 181 | 130 | 272 | 1.38e-12 | 65.5 |
| MsG0480023120.01.T01 | MTR_1g037250 | 31.013 | 158 | 83 | 4 | 18 | 169 | 161 | 298 | 2.05e-12 | 65.1 |
| MsG0480023120.01.T01 | MTR_0068s0080 | 31.373 | 153 | 84 | 3 | 18 | 169 | 160 | 292 | 2.06e-12 | 65.1 |
| MsG0480023120.01.T01 | MTR_0068s0080 | 31.373 | 153 | 84 | 3 | 18 | 169 | 160 | 292 | 2.08e-12 | 65.1 |
| MsG0480023120.01.T01 | MTR_2g089975 | 30.065 | 153 | 86 | 3 | 18 | 169 | 160 | 292 | 2.31e-12 | 65.1 |
| MsG0480023120.01.T01 | MTR_1g036800 | 31.373 | 153 | 84 | 3 | 18 | 169 | 160 | 292 | 2.42e-12 | 65.1 |
| MsG0480023120.01.T01 | MTR_3g063010 | 27.624 | 181 | 105 | 5 | 6 | 181 | 138 | 297 | 2.88e-12 | 64.7 |
| MsG0480023120.01.T01 | MTR_3g062970 | 30.723 | 166 | 89 | 6 | 19 | 181 | 155 | 297 | 6.76e-12 | 63.5 |
| MsG0480023120.01.T01 | MTR_3g062220 | 31.098 | 164 | 91 | 5 | 19 | 181 | 155 | 297 | 7.69e-12 | 63.5 |
| MsG0480023120.01.T01 | MTR_3g062420 | 27.322 | 183 | 103 | 5 | 6 | 178 | 138 | 300 | 1.04e-11 | 63.2 |
| MsG0480023120.01.T01 | MTR_0043s0110 | 26.519 | 181 | 107 | 5 | 6 | 181 | 137 | 296 | 3.60e-11 | 61.6 |
| MsG0480023120.01.T01 | MTR_3g048690 | 33.548 | 155 | 83 | 5 | 28 | 181 | 101 | 236 | 4.22e-11 | 61.2 |
| MsG0480023120.01.T01 | MTR_2g071910 | 40.909 | 66 | 39 | 0 | 116 | 181 | 11 | 76 | 9.54e-11 | 60.1 |
| MsG0480023120.01.T01 | MTR_2g071970 | 40.909 | 66 | 39 | 0 | 116 | 181 | 11 | 76 | 9.54e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAAGATTCAAACACTAAT+TGG | 0.231039 | 4:-81131229 | MsG0480023120.01.T01:CDS |
| TTTGTAATGTCTGGCATCTT+TGG | 0.256799 | 4:+81131247 | None:intergenic |
| ACATTCTTGGCATGGATGAT+AGG | 0.286781 | 4:-81131423 | MsG0480023120.01.T01:CDS |
| TTTATCAACCTTCATATGTT+TGG | 0.289537 | 4:+81130946 | None:intergenic |
| TTGGGTTACACTTACATTCT+TGG | 0.300550 | 4:-81131436 | MsG0480023120.01.T01:CDS |
| CTTCATGGTAGACTCGATTT+AGG | 0.305562 | 4:-81131046 | MsG0480023120.01.T01:CDS |
| GCTCACATCTACAAGCTTAG+AGG | 0.306403 | 4:-81130975 | MsG0480023120.01.T01:CDS |
| GATGGCGACATTGGGTCGTT+TGG | 0.320419 | 4:-81131455 | MsG0480023120.01.T01:CDS |
| ATGGCGACATTGGGTCGTTT+GGG | 0.373709 | 4:-81131454 | MsG0480023120.01.T01:CDS |
| ATTGTACTGTATGATCTTCA+TGG | 0.395468 | 4:-81131061 | MsG0480023120.01.T01:CDS |
| TCAACTTGTTGCATGTGTTT+TGG | 0.403016 | 4:-81131160 | MsG0480023120.01.T01:CDS |
| GGAGAGATTGGTATCCCCTT+TGG | 0.411484 | 4:-81131025 | MsG0480023120.01.T01:CDS |
| GACCAAGGAGAAAATATAAT+CGG | 0.412405 | 4:-81131370 | MsG0480023120.01.T01:CDS |
| ACACTAATTGGTGATGCAAT+AGG | 0.421541 | 4:-81131217 | MsG0480023120.01.T01:CDS |
| GTTTGAATCTTTGTAATGTC+TGG | 0.425122 | 4:+81131238 | None:intergenic |
| CATTCTTGGCATGGATGATA+GGG | 0.452145 | 4:-81131422 | MsG0480023120.01.T01:CDS |
| AGACTCGATTTAGGAGAGAT+TGG | 0.462060 | 4:-81131037 | MsG0480023120.01.T01:CDS |
| GGGGAACAGATGGCGACATT+GGG | 0.475586 | 4:-81131463 | None:intergenic |
| TGGGGAACAGATGGCGACAT+TGG | 0.485299 | 4:-81131464 | None:intergenic |
| TCATTATGATCTTCACCAAA+GGG | 0.503786 | 4:+81131010 | None:intergenic |
| GAAGATCATACAGTACAATC+AGG | 0.516562 | 4:+81131065 | None:intergenic |
| TTACACTTACATTCTTGGCA+TGG | 0.531639 | 4:-81131431 | MsG0480023120.01.T01:CDS |
| AAAATATAATCGGATTATGC+GGG | 0.561138 | 4:-81131360 | MsG0480023120.01.T01:CDS |
| ATCATTATGATCTTCACCAA+AGG | 0.565394 | 4:+81131009 | None:intergenic |
| GAAAATATAATCGGATTATG+CGG | 0.588031 | 4:-81131361 | MsG0480023120.01.T01:CDS |
| TCTGAACTTTGATGATAAGA+CGG | 0.612552 | 4:-81131194 | MsG0480023120.01.T01:CDS |
| GCTTTAAGAAAGAAAGACCA+AGG | 0.634593 | 4:-81131385 | MsG0480023120.01.T01:CDS |
| CATTATGATCTTCACCAAAG+GGG | 0.682494 | 4:+81131011 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATATAATCGGATTATGC+GGG | - | Chr4:81131016-81131035 | MsG0480023120.01.T01:CDS | 25.0% |
| ! | ACAAAGATTCAAACACTAAT+TGG | - | Chr4:81131147-81131166 | MsG0480023120.01.T01:CDS | 25.0% |
| ! | GAAAATATAATCGGATTATG+CGG | - | Chr4:81131015-81131034 | MsG0480023120.01.T01:CDS | 25.0% |
| ! | TTTATCAACCTTCATATGTT+TGG | + | Chr4:81131433-81131452 | None:intergenic | 25.0% |
| ATCATTATGATCTTCACCAA+AGG | + | Chr4:81131370-81131389 | None:intergenic | 30.0% | |
| ATTGTACTGTATGATCTTCA+TGG | - | Chr4:81131315-81131334 | MsG0480023120.01.T01:CDS | 30.0% | |
| GACCAAGGAGAAAATATAAT+CGG | - | Chr4:81131006-81131025 | MsG0480023120.01.T01:CDS | 30.0% | |
| GTTTGAATCTTTGTAATGTC+TGG | + | Chr4:81131141-81131160 | None:intergenic | 30.0% | |
| TCATTATGATCTTCACCAAA+GGG | + | Chr4:81131369-81131388 | None:intergenic | 30.0% | |
| ! | ATCCGATTATATTTTCTCCT+TGG | + | Chr4:81131011-81131030 | None:intergenic | 30.0% |
| ! | GAATGCTTGACTATTTTATC+TGG | + | Chr4:81131042-81131061 | None:intergenic | 30.0% |
| ! | TCTGAACTTTGATGATAAGA+CGG | - | Chr4:81131182-81131201 | MsG0480023120.01.T01:CDS | 30.0% |
| !! | GGTTTTATCCAAACATATGA+AGG | - | Chr4:81131422-81131441 | MsG0480023120.01.T01:CDS | 30.0% |
| CATTATGATCTTCACCAAAG+GGG | + | Chr4:81131368-81131387 | None:intergenic | 35.0% | |
| GAAGATCATACAGTACAATC+AGG | + | Chr4:81131314-81131333 | None:intergenic | 35.0% | |
| TTACACTTACATTCTTGGCA+TGG | - | Chr4:81130945-81130964 | MsG0480023120.01.T01:CDS | 35.0% | |
| TTGGGTTACACTTACATTCT+TGG | - | Chr4:81130940-81130959 | MsG0480023120.01.T01:CDS | 35.0% | |
| TTTGTAATGTCTGGCATCTT+TGG | + | Chr4:81131132-81131151 | None:intergenic | 35.0% | |
| !! | ACACTAATTGGTGATGCAAT+AGG | - | Chr4:81131159-81131178 | MsG0480023120.01.T01:CDS | 35.0% |
| !! | GCTTTAAGAAAGAAAGACCA+AGG | - | Chr4:81130991-81131010 | MsG0480023120.01.T01:CDS | 35.0% |
| !!! | TCAACTTGTTGCATGTGTTT+TGG | - | Chr4:81131216-81131235 | MsG0480023120.01.T01:CDS | 35.0% |
| ACATTCTTGGCATGGATGAT+AGG | - | Chr4:81130953-81130972 | MsG0480023120.01.T01:CDS | 40.0% | |
| AGACTCGATTTAGGAGAGAT+TGG | - | Chr4:81131339-81131358 | MsG0480023120.01.T01:CDS | 40.0% | |
| CATTCTTGGCATGGATGATA+GGG | - | Chr4:81130954-81130973 | MsG0480023120.01.T01:CDS | 40.0% | |
| CTTCATGGTAGACTCGATTT+AGG | - | Chr4:81131330-81131349 | MsG0480023120.01.T01:CDS | 40.0% | |
| GCTCACATCTACAAGCTTAG+AGG | - | Chr4:81131401-81131420 | MsG0480023120.01.T01:CDS | 45.0% | |
| ATGGCGACATTGGGTCGTTT+GGG | - | Chr4:81130922-81130941 | MsG0480023120.01.T01:CDS | 50.0% | |
| GGAGAGATTGGTATCCCCTT+TGG | - | Chr4:81131351-81131370 | MsG0480023120.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 81130922 | 81131476 | 81130922 | ID=MsG0480023120.01;Name=MsG0480023120.01 |
| Chr4 | mRNA | 81130922 | 81131476 | 81130922 | ID=MsG0480023120.01.T01;Parent=MsG0480023120.01;Name=MsG0480023120.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|184 |
| Chr4 | exon | 81130922 | 81131476 | 81130922 | ID=MsG0480023120.01.T01:exon:2362;Parent=MsG0480023120.01.T01 |
| Chr4 | CDS | 81130922 | 81131476 | 81130922 | ID=MsG0480023120.01.T01:cds;Parent=MsG0480023120.01.T01 |
| Gene Sequence |
| Protein sequence |
