AlfalfaGEDB | MsG0480023121.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023121.01

KEGG: -
GO: GO:0043531
PFAM: PF00931

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023121.01.T01	XP_024629426.1	92.188	192	11	3	1	190	1	190	2.60E-120	348

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023121.01.T01	G7L7A4	92.188	192	11	3	1	190	65	254	1.28e-119	348

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048065.01	MsG0480023121.01	0.812784	3.560700e-51	2.284591e-48
MsG0080048660.01	MsG0480023121.01	0.805260	1.454276e-49	7.658499e-47
MsG0080048773.01	MsG0480023121.01	0.848166	7.286185e-60	1.325321e-56
MsG0080048849.01	MsG0480023121.01	0.817438	3.297412e-52	2.401429e-49
MsG0080048878.01	MsG0480023121.01	0.834007	3.844236e-56	4.503454e-53
MsG0080049068.01	MsG0480023121.01	0.825909	3.623930e-54	3.348025e-51
MsG0180000599.01	MsG0480023121.01	0.806777	6.975470e-50	3.819132e-47
MsG0180000785.01	MsG0480023121.01	0.833347	5.619631e-56	6.454609e-53
MsG0180001610.01	MsG0480023121.01	0.804525	2.071717e-49	1.070533e-46
MsG0180001704.01	MsG0480023121.01	0.823219	1.558443e-53	1.333556e-50
MsG0180002399.01	MsG0480023121.01	0.818301	2.105427e-52	1.570019e-49
MsG0180002700.01	MsG0480023121.01	0.822359	2.470563e-53	2.063371e-50
MsG0180003353.01	MsG0480023121.01	0.827899	1.212204e-54	1.186077e-51
MsG0180003821.01	MsG0480023121.01	0.812747	3.628020e-51	2.325662e-48
MsG0180004103.01	MsG0480023121.01	0.823668	1.223372e-53	1.060346e-50
MsG0180004246.01	MsG0480023121.01	0.819404	1.182376e-52	9.089879e-50
MsG0180004493.01	MsG0480023121.01	0.834558	2.796906e-56	3.331290e-53
MsG0180004605.01	MsG0480023121.01	-0.803528	3.338983e-49	1.681895e-46
MsG0180004759.01	MsG0480023121.01	-0.803354	3.628226e-49	1.819255e-46
MsG0180005248.01	MsG0480023121.01	-0.809162	2.168293e-50	1.263770e-47
MsG0180005807.01	MsG0480023121.01	0.822557	2.222567e-53	1.866775e-50
MsG0180006031.01	MsG0480023121.01	0.815558	8.691388e-52	6.012119e-49
MsG0280010564.01	MsG0480023121.01	0.814587	1.427834e-51	9.619451e-49
MsG0280010736.01	MsG0480023121.01	0.805563	1.256625e-49	6.669401e-47
MsG0280011255.01	MsG0480023121.01	0.818948	1.501765e-52	1.140188e-49
MsG0480018584.01	MsG0480023121.01	0.830777	2.425681e-55	2.581294e-52
MsG0480018850.01	MsG0480023121.01	0.804155	2.474481e-49	1.266618e-46
MsG0480018911.01	MsG0480023121.01	0.817090	3.949430e-52	2.848842e-49
MsG0480019007.01	MsG0480023121.01	0.811974	5.351203e-51	3.359968e-48
MsG0480020024.01	MsG0480023121.01	0.822896	1.852976e-53	1.571281e-50
MsG0480020690.01	MsG0480023121.01	0.828737	7.612649e-55	7.632076e-52
MsG0480021248.01	MsG0480023121.01	0.812309	4.523815e-51	2.866399e-48
MsG0480022149.01	MsG0480023121.01	0.829262	5.679946e-55	5.784143e-52
MsG0480022624.01	MsG0480023121.01	0.809096	2.240198e-50	1.303352e-47
MsG0480023073.01	MsG0480023121.01	0.816736	4.743182e-52	3.388324e-49
MsG0480023120.01	MsG0480023121.01	0.803181	3.940976e-49	1.967383e-46
MsG0480023121.01	MsG0580024628.01	0.812634	3.841419e-51	2.455132e-48
MsG0480023121.01	MsG0580024790.01	-0.800658	1.299985e-48	6.087004e-46
MsG0480023121.01	MsG0580025055.01	0.819863	9.290243e-53	7.234468e-50
MsG0480023121.01	MsG0580025407.01	-0.819661	1.033161e-52	8.000029e-50
MsG0480023121.01	MsG0580025561.01	-0.817939	2.541706e-52	1.876601e-49
MsG0480023121.01	MsG0580025627.01	0.832260	1.046103e-55	1.162954e-52
MsG0480023121.01	MsG0580025971.01	0.818168	2.256520e-52	1.676514e-49
MsG0480023121.01	MsG0580026159.01	-0.830679	2.563936e-55	2.720609e-52
MsG0480023121.01	MsG0580027394.01	0.800179	1.627498e-48	7.528215e-46
MsG0480023121.01	MsG0580028428.01	0.806166	9.384537e-50	5.058705e-47
MsG0480023121.01	MsG0580029039.01	0.820439	6.855391e-53	5.424573e-50
MsG0480023121.01	MsG0580029529.01	0.835386	1.729342e-56	2.111539e-53
MsG0480023121.01	MsG0580029734.01	0.800154	1.646716e-48	7.612200e-46
MsG0480023121.01	MsG0580029879.01	0.820595	6.313553e-53	5.016910e-50
MsG0480023121.01	MsG0680030680.01	0.801560	8.500216e-49	4.071865e-46
MsG0480023121.01	MsG0680031010.01	0.806700	7.241481e-50	3.956882e-47
MsG0480023121.01	MsG0680031146.01	0.806213	9.175348e-50	4.951840e-47
MsG0480023121.01	MsG0680031640.01	0.803404	3.542309e-49	1.778569e-46
MsG0480023121.01	MsG0680031702.01	0.804532	2.065080e-49	1.067291e-46
MsG0480023121.01	MsG0680032827.01	0.806319	8.715238e-50	4.716005e-47
MsG0480023121.01	MsG0680032832.01	0.803724	3.040519e-49	1.539198e-46
MsG0480023121.01	MsG0680033377.01	0.820791	5.690084e-53	4.546612e-50
MsG0480023121.01	MsG0780035960.01	0.827914	1.202047e-54	1.176677e-51
MsG0480023121.01	MsG0780035965.01	0.806027	1.004065e-49	5.393026e-47
MsG0480023121.01	MsG0780036443.01	0.813045	3.122701e-51	2.017525e-48
MsG0480023121.01	MsG0780036659.01	0.820796	5.674430e-53	4.534765e-50
MsG0480023121.01	MsG0780037157.01	0.805509	1.289764e-49	6.835730e-47
MsG0480023121.01	MsG0780037341.01	0.818109	2.326683e-52	1.725839e-49
MsG0480023121.01	MsG0780037395.01	0.827239	1.746338e-54	1.676218e-51
MsG0480023121.01	MsG0780038056.01	0.803436	3.489886e-49	1.753647e-46
MsG0480023121.01	MsG0780038584.01	-0.811869	5.641090e-51	3.531860e-48
MsG0480023121.01	MsG0780038744.01	0.803179	3.943692e-49	1.968675e-46
MsG0480023121.01	MsG0780038784.01	0.813431	2.568126e-51	1.676811e-48
MsG0480023121.01	MsG0780039369.01	0.804559	2.037907e-49	1.054027e-46
MsG0480023121.01	MsG0780040278.01	0.800049	1.729568e-48	7.974129e-46
MsG0480023121.01	MsG0780040388.01	0.812595	3.917764e-51	2.501292e-48
MsG0480023121.01	MsG0780041091.01	0.814825	1.265031e-51	8.577155e-49
MsG0480023121.01	MsG0780041440.01	0.802880	4.546649e-49	2.252423e-46
MsG0480023121.01	MsG0780041747.01	0.809547	1.792772e-50	1.055660e-47
MsG0480023121.01	MsG0880041861.01	0.815496	8.972599e-52	6.196428e-49
MsG0480023121.01	MsG0880042022.01	0.804220	2.398356e-49	1.229637e-46
MsG0480023121.01	MsG0880043955.01	0.830170	3.415500e-55	3.570793e-52
MsG0480023121.01	MsG0880043965.01	0.808686	2.740963e-50	1.577343e-47
MsG0480023121.01	MsG0880044005.01	0.830372	3.047178e-55	3.204451e-52
MsG0480023121.01	MsG0880044392.01	0.803717	3.050793e-49	1.544086e-46
MsG0480023121.01	MsG0880044487.01	0.832127	1.128379e-55	1.249603e-52
MsG0480023121.01	MsG0880046765.01	0.809541	1.797954e-50	1.058547e-47
MsG0480023121.01	MsG0880047394.01	0.820504	6.622240e-53	5.249348e-50
MsG0480023121.01	MsG0880047422.01	-0.814091	1.838189e-51	1.221815e-48
MsG0480023121.01	MsG0880047481.01	-0.819220	1.301836e-52	9.957283e-50
MsG0480023121.01	MsG0880047538.01	0.827154	1.830241e-54	1.752289e-51
MsG0480023121.01	MsG0880047770.01	0.801182	1.015993e-48	4.820790e-46
MsG0380015784.01	MsG0480023121.01	0.834145	3.549820e-56	4.175206e-53
MsG0380015896.01	MsG0480023121.01	0.835898	1.282976e-56	1.590693e-53
MsG0380016347.01	MsG0480023121.01	-0.805983	1.025547e-49	5.502452e-47
MsG0380016479.01	MsG0480023121.01	0.805708	1.171434e-49	6.241180e-47
MsG0380016540.01	MsG0480023121.01	0.804553	2.043708e-49	1.056859e-46
MsG0380016864.01	MsG0480023121.01	0.806501	7.978106e-50	4.337243e-47
MsG0380017293.01	MsG0480023121.01	0.802011	6.867288e-49	3.327528e-46
MsG0380017931.01	MsG0480023121.01	0.812270	4.613872e-51	2.920459e-48
MsG0380018037.01	MsG0480023121.01	0.818814	1.610450e-52	1.218031e-49
MsG0280006996.01	MsG0480023121.01	0.822931	1.818993e-53	1.544021e-50
MsG0280007193.01	MsG0480023121.01	-0.805094	1.575875e-49	8.263785e-47
MsG0280007647.01	MsG0480023121.01	0.815177	1.056754e-51	7.233228e-49
MsG0280007771.01	MsG0480023121.01	0.802857	4.598108e-49	2.276548e-46
MsG0280007903.01	MsG0480023121.01	0.835890	1.289512e-56	1.598365e-53
MsG0280007976.01	MsG0480023121.01	0.811550	6.619366e-51	4.109587e-48
MsG0280008709.01	MsG0480023121.01	0.802665	5.037431e-49	2.481809e-46
MsG0280008928.01	MsG0480023121.01	0.802269	6.078465e-49	2.964740e-46
MsG0280009256.01	MsG0480023121.01	0.801436	9.014078e-49	4.304541e-46
MsG0280009756.01	MsG0480023121.01	0.800193	1.616752e-48	7.481243e-46
MsG0280009953.01	MsG0480023121.01	0.808195	3.489388e-50	1.982522e-47
MsG0280010503.01	MsG0480023121.01	0.801855	7.393206e-49	3.568306e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023121.01.T01	MTR_8g087590	92.188	192	11	3	1	190	65	254	3.26e-123	348
MsG0480023121.01.T01	MTR_8g087650	80.328	183	34	1	13	193	13	195	2.53e-106	303
MsG0480023121.01.T01	MTR_8g087610	81.714	175	32	0	19	193	35	209	1.24e-104	300
MsG0480023121.01.T01	MTR_8g087640	79.888	179	33	1	11	189	58	233	7.24e-99	286
MsG0480023121.01.T01	MTR_8g087640	79.888	179	33	1	11	189	102	277	9.09e-99	287
MsG0480023121.01.T01	MTR_8g087630	78.333	180	37	1	14	193	14	191	5.30e-98	282
MsG0480023121.01.T01	MTR_8g087570	74.444	180	27	2	11	190	94	254	1.81e-85	253
MsG0480023121.01.T01	MTR_8g087570	72.973	185	31	2	6	190	51	216	1.88e-85	251
MsG0480023121.01.T01	MTR_8g087570	72.973	185	31	2	6	190	51	216	1.88e-85	251
MsG0480023121.01.T01	MTR_8g087600	65.789	152	23	1	25	176	7	129	2.56e-63	198
MsG0480023121.01.T01	MTR_8g087600	61.392	158	30	3	38	193	178	306	3.81e-54	174
MsG0480023121.01.T01	MTR_8g087555	66.892	148	21	1	46	193	4	123	8.79e-57	175
MsG0480023121.01.T01	MTR_3g013790	35.714	168	85	4	29	192	152	300	7.35e-23	95.5
MsG0480023121.01.T01	MTR_3g013790	35.714	168	85	4	29	192	152	300	1.01e-22	95.5
MsG0480023121.01.T01	MTR_2g072820	35.821	134	68	1	57	190	187	302	1.48e-21	92.0
MsG0480023121.01.T01	MTR_2g072080	37.313	134	66	1	57	190	189	304	1.64e-21	92.0
MsG0480023121.01.T01	MTR_2g072020	35.821	134	68	1	57	190	189	304	2.12e-21	91.7
MsG0480023121.01.T01	MTR_2g072150	35.821	134	68	1	57	190	189	304	3.85e-21	90.9
MsG0480023121.01.T01	MTR_1g069615	37.903	124	59	2	63	186	79	184	6.99e-21	85.5
MsG0480023121.01.T01	MTR_2g072030	35.542	166	86	3	26	190	159	304	7.16e-21	90.1
MsG0480023121.01.T01	MTR_2g072110	35.821	134	68	1	57	190	189	304	3.22e-20	88.2
MsG0480023121.01.T01	MTR_2g072110	35.821	134	68	1	57	190	189	304	3.35e-20	88.2
MsG0480023121.01.T01	MTR_1g036750	39.837	123	55	2	64	186	165	268	6.35e-20	87.4
MsG0480023121.01.T01	MTR_2g071820	35.526	152	77	3	29	179	162	293	1.09e-19	86.3
MsG0480023121.01.T01	MTR_1g037010	39.837	123	55	2	64	186	197	300	1.23e-19	86.7
MsG0480023121.01.T01	MTR_2g071940	36.719	128	63	2	63	190	199	308	8.16e-19	84.0
MsG0480023121.01.T01	MTR_1g037150	37.805	164	76	6	26	186	147	287	9.28e-19	84.0
MsG0480023121.01.T01	MTR_2g071890	34.694	147	77	2	34	179	165	293	9.91e-19	84.0
MsG0480023121.01.T01	MTR_2g071870	36.719	128	63	2	63	190	199	308	1.32e-18	83.6
MsG0480023121.01.T01	MTR_1g041550	35.976	164	79	4	26	186	160	300	1.56e-18	83.2
MsG0480023121.01.T01	MTR_1g044020	36.196	163	78	4	27	186	161	300	1.78e-18	83.2
MsG0480023121.01.T01	MTR_2g072090	35.938	128	64	2	63	190	199	308	2.07e-18	82.8
MsG0480023121.01.T01	MTR_1g036860	33.523	176	88	5	19	186	145	299	2.63e-18	82.4
MsG0480023121.01.T01	MTR_0006s0240	37.805	164	76	6	26	186	160	300	2.86e-18	82.4
MsG0480023121.01.T01	MTR_0069s0040	34.146	164	82	4	26	186	159	299	3.29e-18	82.4
MsG0480023121.01.T01	MTR_2g071900	35.938	128	64	2	63	190	195	304	3.55e-18	82.0
MsG0480023121.01.T01	MTR_7g026250	32.386	176	94	6	18	190	119	272	4.89e-18	81.6
MsG0480023121.01.T01	MTR_0006s0080	39.024	123	56	2	64	186	197	300	5.44e-18	81.6
MsG0480023121.01.T01	MTR_1g051585	36.585	123	59	2	64	186	196	299	8.58e-18	81.3
MsG0480023121.01.T01	MTR_0006s0230	39.837	123	55	3	64	186	197	300	9.04e-18	81.3
MsG0480023121.01.T01	MTR_1g037130	36.522	115	55	1	64	178	196	292	1.50e-17	80.5
MsG0480023121.01.T01	MTR_2g071980	35.156	128	65	1	63	190	195	304	2.49e-17	79.7
MsG0480023121.01.T01	MTR_2g071850	35.156	128	65	2	63	190	199	308	6.44e-17	78.6
MsG0480023121.01.T01	MTR_3g063010	31.818	176	95	6	18	190	144	297	6.47e-17	78.6
MsG0480023121.01.T01	MTR_3g062420	31.902	163	87	5	18	177	144	285	1.59e-16	77.4
MsG0480023121.01.T01	MTR_6g463150	36.697	109	51	2	64	172	196	286	2.32e-16	77.0
MsG0480023121.01.T01	MTR_3g062220	31.818	176	95	6	18	190	144	297	4.31e-16	76.3
MsG0480023121.01.T01	MTR_3g062970	30.682	176	97	6	18	190	144	297	5.63e-16	75.9
MsG0480023121.01.T01	MTR_5g062770	29.448	163	93	4	29	190	142	283	1.14e-15	74.3
MsG0480023121.01.T01	MTR_8g059345	38.211	123	57	2	64	186	196	299	1.33e-15	74.7
MsG0480023121.01.T01	MTR_0006s0140	34.959	123	61	2	64	186	196	299	2.02e-15	73.6
MsG0480023121.01.T01	MTR_1g037250	33.918	171	82	6	24	186	158	305	6.03e-15	72.8
MsG0480023121.01.T01	MTR_0043s0110	30.286	175	99	5	18	190	143	296	6.93e-15	72.4
MsG0480023121.01.T01	MTR_3g467640	30.769	143	80	2	36	177	6	130	2.08e-14	71.2
MsG0480023121.01.T01	MTR_0068s0080	33.333	168	82	6	24	186	157	299	2.68e-14	70.9
MsG0480023121.01.T01	MTR_0912s0010	29.609	179	95	6	18	190	143	296	2.72e-14	70.9
MsG0480023121.01.T01	MTR_1g036800	33.333	168	82	6	24	186	157	299	3.03e-14	70.9
MsG0480023121.01.T01	MTR_0068s0080	33.333	168	82	6	24	186	157	299	3.54e-14	70.5
MsG0480023121.01.T01	MTR_2g089975	31.481	162	89	4	26	186	159	299	3.65e-14	70.5
MsG0480023121.01.T01	MTR_3g451650	29.714	175	100	5	18	190	143	296	1.21e-13	68.9
MsG0480023121.01.T01	MTR_3g062990	29.341	167	86	6	18	177	202	343	1.89e-13	68.6
MsG0480023121.01.T01	MTR_3g464840	29.762	168	87	5	28	190	156	297	2.41e-13	68.2
MsG0480023121.01.T01	MTR_2g071910	48.387	62	32	0	125	186	11	72	3.50e-13	67.4
MsG0480023121.01.T01	MTR_2g071970	48.387	62	32	0	125	186	11	72	3.50e-13	67.4
MsG0480023121.01.T01	MTR_3g451150	27.841	176	102	6	18	190	144	297	6.55e-13	67.0
MsG0480023121.01.T01	MTR_3g452250	27.841	176	102	6	18	190	144	297	6.55e-13	67.0
MsG0480023121.01.T01	MTR_0067s0070	28.358	134	77	2	57	190	183	297	1.34e-12	65.9
MsG0480023121.01.T01	MTR_3g466750	28.409	176	102	6	17	190	130	283	2.11e-12	65.5
MsG0480023121.01.T01	MTR_3g048690	32.231	121	63	2	70	190	135	236	3.07e-12	65.1
MsG0480023121.01.T01	MTR_5g092410	29.936	157	78	6	24	174	148	278	3.85e-12	64.3
MsG0480023121.01.T01	MTR_3g466580	28.571	168	90	5	28	190	156	298	6.98e-12	63.9
MsG0480023121.01.T01	MTR_3g464920	28.402	169	91	5	27	190	155	298	1.05e-11	63.5
MsG0480023121.01.T01	MTR_6g464050	37.500	96	42	2	83	178	832	909	1.46e-11	63.2
MsG0480023121.01.T01	MTR_3g062010	28.834	163	94	4	29	190	156	297	2.07e-11	62.4
MsG0480023121.01.T01	MTR_3g464950	26.404	178	100	6	18	189	145	297	2.44e-11	62.4
MsG0480023121.01.T01	MTR_3g464870	27.219	169	93	5	27	190	155	298	3.76e-11	62.0
MsG0480023121.01.T01	MTR_3g464870	27.219	169	93	5	27	190	155	298	4.04e-11	61.6
MsG0480023121.01.T01	MTR_3g053200	27.564	156	94	2	36	190	167	304	4.16e-11	61.6
MsG0480023121.01.T01	MTR_1g031830	27.273	154	83	5	29	177	202	331	4.99e-11	61.6
MsG0480023121.01.T01	MTR_3g464810	27.044	159	88	4	36	189	162	297	5.74e-11	61.2
MsG0480023121.01.T01	MTR_5g085400	30.539	167	88	6	28	191	160	301	9.68e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 33 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGCAATCCGGGAACAGTTT+GGG	0.217269	4:-81133935	MsG0480023121.01.T01:CDS
GACCAAGGAGAAAATATAAT+TGG	0.266280	4:-81133845	MsG0480023121.01.T01:CDS
ACAAAGATTCAAACACAAAT+TGG	0.268354	4:-81133704	MsG0480023121.01.T01:CDS
GTTTGAATCTTTGTAATATC+TGG	0.300279	4:+81133713	None:intergenic
CAGTTGCTGTGACAATCTTT+TGG	0.304172	4:+81133747	None:intergenic
TCGGCAATCCGGGAACAGTT+TGG	0.321768	4:-81133936	MsG0480023121.01.T01:CDS
ATTGCTCACATCTACAAGTT+TGG	0.339784	4:-81133453	MsG0480023121.01.T01:CDS
CATTATGTCGTTTGAGAGAT+TGG	0.354751	4:-81133960	None:intergenic
GTTGTACTGTATGATCTTCA+TGG	0.377791	4:-81133536	MsG0480023121.01.T01:CDS
CCCTTTGGTGAAGATCATAA+TGG	0.415861	4:-81133485	MsG0480023121.01.T01:CDS
AGTGAGATTGGTATCCCCTT+TGG	0.416147	4:-81133500	MsG0480023121.01.T01:CDS
TATTCCCAGCGTGGATGATA+AGG	0.422594	4:-81133903	MsG0480023121.01.T01:CDS
ACACAAATTGGTGATGCAAT+AGG	0.433183	4:-81133692	MsG0480023121.01.T01:CDS
GAGAGATTGGTTCGGCAATC+CGG	0.440927	4:-81133947	MsG0480023121.01.T01:CDS
GGCAATCCGGGAACAGTTTG+GGG	0.442842	4:-81133934	MsG0480023121.01.T01:CDS
AGAGATTGGTTCGGCAATCC+GGG	0.458252	4:-81133946	MsG0480023121.01.T01:CDS
AGACTCGATTTAAGTGAGAT+TGG	0.459620	4:-81133512	MsG0480023121.01.T01:CDS
GCTCACATCTACAAGTTTGG+AGG	0.498220	4:-81133450	MsG0480023121.01.T01:CDS
TTTATGAACCTTCATTTGCT+CGG	0.509437	4:+81133421	None:intergenic
AAAATATAATTGGATTATGC+GGG	0.519176	4:-81133835	MsG0480023121.01.T01:CDS
CCATTATGATCTTCACCAAA+GGG	0.522614	4:+81133485	None:intergenic
GAAAATATAATTGGATTATG+CGG	0.532748	4:-81133836	MsG0480023121.01.T01:CDS
TGTCGTTTGAGAGATTGGTT+CGG	0.553620	4:-81133955	MsG0480023121.01.T01:CDS
ATGCAACAAGTTGATTCAGC+CGG	0.577268	4:+81133644	None:intergenic
TTAACCTTATCATCCACGCT+GGG	0.579493	4:+81133899	None:intergenic
GCAATCCGGGAACAGTTTGG+GGG	0.584978	4:-81133933	MsG0480023121.01.T01:CDS
ACCATTATGATCTTCACCAA+AGG	0.590609	4:+81133484	None:intergenic
GCACTAAGAGTGAGAGACCA+AGG	0.592850	4:-81133860	MsG0480023121.01.T01:CDS
TTTAACCTTATCATCCACGC+TGG	0.639042	4:+81133898	None:intergenic
GAAGATCATACAGTACAACT+AGG	0.653715	4:+81133540	None:intergenic
GGGCATTTATATTCCCAGCG+TGG	0.677027	4:-81133912	MsG0480023121.01.T01:CDS
CAATCCGGGAACAGTTTGGG+GGG	0.685818	4:-81133932	MsG0480023121.01.T01:CDS
CATTATGATCTTCACCAAAG+GGG	0.695532	4:+81133486	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATATAATTGGATTATGC+GGG	-	Chr4:81133518-81133537	MsG0480023121.01.T01:CDS	20.0%
!!	GAAAATATAATTGGATTATG+CGG	-	Chr4:81133517-81133536	MsG0480023121.01.T01:CDS	20.0%
!!!	TCTGAATTTTGATGATAAAA+CGG	-	Chr4:81133684-81133703	MsG0480023121.01.T01:CDS	20.0%
!!!	TTTTGGTAATAGAAAAAGAA+TGG	-	Chr4:81133735-81133754	MsG0480023121.01.T01:CDS	20.0%
!	ACAAAGATTCAAACACAAAT+TGG	-	Chr4:81133649-81133668	MsG0480023121.01.T01:CDS	25.0%
!	GTTTGAATCTTTGTAATATC+TGG	+	Chr4:81133643-81133662	None:intergenic	25.0%
!!	ATCCAATTATATTTTCTCCT+TGG	+	Chr4:81133513-81133532	None:intergenic	25.0%
	GACCAAGGAGAAAATATAAT+TGG	-	Chr4:81133508-81133527	MsG0480023121.01.T01:CDS	30.0%
	TTTATGAACCTTCATTTGCT+CGG	+	Chr4:81133935-81133954	None:intergenic	30.0%
!!	TTTTGATGATAAAACGGATC+CGG	-	Chr4:81133690-81133709	MsG0480023121.01.T01:CDS	30.0%
!!!	TCAACTTGTTGCATGTTTTT+TGG	-	Chr4:81133718-81133737	MsG0480023121.01.T01:CDS	30.0%
	ACCATTATGATCTTCACCAA+AGG	+	Chr4:81133872-81133891	None:intergenic	35.0%
	AGACTCGATTTAAGTGAGAT+TGG	-	Chr4:81133841-81133860	MsG0480023121.01.T01:CDS	35.0%
	ATTGCTCACATCTACAAGTT+TGG	-	Chr4:81133900-81133919	MsG0480023121.01.T01:CDS	35.0%
	CATTATGATCTTCACCAAAG+GGG	+	Chr4:81133870-81133889	None:intergenic	35.0%
	CCATTATGATCTTCACCAAA+GGG	+	Chr4:81133871-81133890	None:intergenic	35.0%
	GAAGATCATACAGTACAACT+AGG	+	Chr4:81133816-81133835	None:intergenic	35.0%
	GTTGTACTGTATGATCTTCA+TGG	-	Chr4:81133817-81133836	MsG0480023121.01.T01:CDS	35.0%
!!	ACACAAATTGGTGATGCAAT+AGG	-	Chr4:81133661-81133680	MsG0480023121.01.T01:CDS	35.0%
!!!	GAATGCTTGACTCTTTTTTC+TGG	+	Chr4:81133544-81133563	None:intergenic	35.0%
	CCCTTTGGTGAAGATCATAA+TGG	-	Chr4:81133868-81133887	MsG0480023121.01.T01:CDS	40.0%
	TTAACCTTATCATCCACGCT+GGG	+	Chr4:81133457-81133476	None:intergenic	40.0%
	TTTAACCTTATCATCCACGC+TGG	+	Chr4:81133458-81133477	None:intergenic	40.0%
!	CAGTTGCTGTGACAATCTTT+TGG	+	Chr4:81133609-81133628	None:intergenic	40.0%
!	TGTCGTTTGAGAGATTGGTT+CGG	-	Chr4:81133398-81133417	MsG0480023121.01.T01:CDS	40.0%
!!	ATGCAACAAGTTGATTCAGC+CGG	+	Chr4:81133712-81133731	None:intergenic	40.0%
!!	GGTTTTATCCGAGCAAATGA+AGG	-	Chr4:81133924-81133943	MsG0480023121.01.T01:CDS	40.0%
	AGTGAGATTGGTATCCCCTT+TGG	-	Chr4:81133853-81133872	MsG0480023121.01.T01:CDS	45.0%
	GCTCACATCTACAAGTTTGG+AGG	-	Chr4:81133903-81133922	MsG0480023121.01.T01:CDS	45.0%
	TATTCCCAGCGTGGATGATA+AGG	-	Chr4:81133450-81133469	MsG0480023121.01.T01:CDS	45.0%
	GCACTAAGAGTGAGAGACCA+AGG	-	Chr4:81133493-81133512	MsG0480023121.01.T01:CDS	50.0%
	GGGCATTTATATTCCCAGCG+TGG	-	Chr4:81133441-81133460	MsG0480023121.01.T01:CDS	50.0%
!!	AGAGATTGGTTCGGCAATCC+GGG	-	Chr4:81133407-81133426	MsG0480023121.01.T01:CDS	50.0%
!!	GAGAGATTGGTTCGGCAATC+CGG	-	Chr4:81133406-81133425	MsG0480023121.01.T01:CDS	50.0%
	AATGCCCCCCAAACTGTTCC+CGG	+	Chr4:81133428-81133447	None:intergenic	55.0%
	CAATCCGGGAACAGTTTGGG+GGG	-	Chr4:81133421-81133440	MsG0480023121.01.T01:CDS	55.0%
	CGGCAATCCGGGAACAGTTT+GGG	-	Chr4:81133418-81133437	MsG0480023121.01.T01:CDS	55.0%
	GCAATCCGGGAACAGTTTGG+GGG	-	Chr4:81133420-81133439	MsG0480023121.01.T01:CDS	55.0%
	GGCAATCCGGGAACAGTTTG+GGG	-	Chr4:81133419-81133438	MsG0480023121.01.T01:CDS	55.0%
	TCGGCAATCCGGGAACAGTT+TGG	-	Chr4:81133417-81133436	MsG0480023121.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	81133397	81133978	81133397	ID=MsG0480023121.01;Name=MsG0480023121.01
Chr4	mRNA	81133397	81133978	81133397	ID=MsG0480023121.01.T01;Parent=MsG0480023121.01;Name=MsG0480023121.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|193
Chr4	exon	81133397	81133978	81133397	ID=MsG0480023121.01.T01:exon:2363;Parent=MsG0480023121.01.T01
Chr4	CDS	81133397	81133978	81133397	ID=MsG0480023121.01.T01:cds;Parent=MsG0480023121.01.T01

Gene Sequence

>MsG0480023121.01.T01

ATGTCGTTTGAGAGATTGGTTCGGCAATCCGGGAACAGTTTGGGGGGCATTTATATTCCCAGCGTGGATGATAAGGTTAAAGAACAAATTATGAATGCACTAAGAGTGAGAGACCAAGGAGAAAATATAATTGGATTATGCGGGCCAGAAAAAAGAGTCAAGCATTCTGCAAAAACAGCTATAAGAAGAGCTGAGAGAGATCAATTGTTCCAAAAGATTGTCACAGCAACTGTGACAAAGAAGCCAGATATTACAAAGATTCAAACACAAATTGGTGATGCAATAGGTCTGAATTTTGATGATAAAACGGATCCGGCTGAATCAACTTGTTGCATGTTTTTTGGTAATAGAAAAAGAATGGCAACTGCAGAAAGAGCTCATCATTTGTGTGCTAAGATGAAAGAGCTGCAAACAGTCCTAGTTGTACTGTATGATCTTCATGGCAGACTCGATTTAAGTGAGATTGGTATCCCCTTTGGTGAAGATCATAATGGTTGCAAGATATTGCTCACATCTACAAGTTTGGAGGTTTTATCCGAGCAAATGAAGGTTCATAAATTGATACAATTGTCAGAGACATGA

Protein sequence

>MsG0480023121.01.T01

MSFERLVRQSGNSLGGIYIPSVDDKVKEQIMNALRVRDQGENIIGLCGPEKRVKHSAKTAIRRAERDQLFQKIVTATVTKKPDITKIQTQIGDAIGLNFDDKTDPAESTCCMFFGNRKRMATAERAHHLCAKMKELQTVLVVLYDLHGRLDLSEIGIPFGEDHNGCKILLTSTSLEVLSEQMKVHKLIQLSET*