Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023154.01.T01 | XP_024629538.1 | 95.565 | 248 | 11 | 0 | 2 | 249 | 3 | 250 | 2.64E-159 | 452 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023154.01.T01 | Q8GYK7 | 59.414 | 239 | 94 | 2 | 14 | 249 | 16 | 254 | 2.73E-98 | 290 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023154.01.T01 | G7L823 | 94.262 | 244 | 14 | 0 | 2 | 245 | 3 | 246 | 5.32e-153 | 437 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480018252.01 | MsG0480023154.01 | -0.806719 | 7.176043e-50 | 3.923089e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023154.01.T01 | MTR_8g088170 | 94.262 | 244 | 14 | 0 | 2 | 245 | 3 | 246 | 1.35e-156 | 437 |
| MsG0480023154.01.T01 | MTR_8g088140 | 42.391 | 92 | 40 | 2 | 11 | 102 | 14 | 92 | 4.38e-17 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023154.01.T01 | AT5G14285 | 59.414 | 239 | 94 | 2 | 14 | 249 | 16 | 254 | 2.78e-99 | 290 |
| MsG0480023154.01.T01 | AT3G25950 | 49.587 | 242 | 115 | 4 | 9 | 245 | 12 | 251 | 7.25e-74 | 225 |
| MsG0480023154.01.T01 | AT3G27270 | 47.083 | 240 | 120 | 3 | 14 | 249 | 13 | 249 | 3.11e-69 | 213 |
| MsG0480023154.01.T01 | AT3G27270 | 47.083 | 240 | 120 | 3 | 14 | 249 | 13 | 249 | 8.49e-69 | 213 |
Find 63 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTACGTGGATTTGGGTTTCT+TGG | 0.194477 | 4:+81512894 | MsG0480023154.01.T01:CDS |
| TATGCTAGCGGGCTTGCTTA+TGG | 0.231439 | 4:+81512863 | MsG0480023154.01.T01:CDS |
| ATGCTAGCGGGCTTGCTTAT+GGG | 0.278734 | 4:+81512864 | MsG0480023154.01.T01:CDS |
| ACCATTCTAAACACAAAGTA+TGG | 0.291122 | 4:-81512833 | None:intergenic |
| GGCTTGTGCCTACGTGGATT+TGG | 0.302504 | 4:+81512885 | MsG0480023154.01.T01:CDS |
| GTGGTGGTTATTTCAGCTAT+TGG | 0.317693 | 4:+81512920 | MsG0480023154.01.T01:CDS |
| TTCCTTGCAATTTACCTAAT+CGG | 0.319525 | 4:+81512332 | MsG0480023154.01.T01:CDS |
| GGCACTCCCGCCGCAATCTT+CGG | 0.342672 | 4:+81512431 | MsG0480023154.01.T01:CDS |
| GATTAAACAGCTGGAGAATT+CGG | 0.368706 | 4:-81512396 | None:intergenic |
| AGGGAAGCGAAGCCGCTGTT+GGG | 0.383271 | 4:-81512476 | None:intergenic |
| GGACGCTCACCGGTGCTTGC+CGG | 0.391009 | 4:+81512723 | MsG0480023154.01.T01:CDS |
| GCTTGTGCCTACGTGGATTT+GGG | 0.392366 | 4:+81512886 | MsG0480023154.01.T01:CDS |
| TGTTTAATCTCCCTCTTTCA+CGG | 0.393105 | 4:+81512410 | MsG0480023154.01.T01:CDS |
| CTCATTAGTGAAAGAAGAAC+TGG | 0.397378 | 4:+81512980 | MsG0480023154.01.T01:CDS |
| TCCATACTTTGTGTTTAGAA+TGG | 0.406880 | 4:+81512832 | MsG0480023154.01.T01:CDS |
| TACGTGGATTTGGGTTTCTT+GGG | 0.411670 | 4:+81512895 | MsG0480023154.01.T01:CDS |
| AGGAAAATCGCTTCGCCGCT+AGG | 0.414084 | 4:+81512747 | MsG0480023154.01.T01:CDS |
| GCTGTTGGGGTCGGAGAAGA+TGG | 0.421410 | 4:-81512462 | None:intergenic |
| ATGGGAAAACTAACAAATCT+TGG | 0.424453 | 4:-81512298 | None:intergenic |
| AAGAACTGGTGCAAAATTAG+AGG | 0.425702 | 4:+81512994 | MsG0480023154.01.T01:CDS |
| TTCTATTGTGAGGGGATTTG+TGG | 0.429394 | 4:+81512808 | MsG0480023154.01.T01:CDS |
| ATAGAATACAACACGTAAAA+CGG | 0.433592 | 4:-81512791 | None:intergenic |
| TCTATTGTGAGGGGATTTGT+GGG | 0.443433 | 4:+81512809 | MsG0480023154.01.T01:CDS |
| CGATGAAGAGAACGTCGCTT+GGG | 0.452772 | 4:-81512580 | None:intergenic |
| TTGGGTTTCTTGGGCTGTGG+TGG | 0.455719 | 4:+81512904 | MsG0480023154.01.T01:CDS |
| CGTGTTGTATTCTATTGTGA+GGG | 0.461748 | 4:+81512799 | MsG0480023154.01.T01:CDS |
| GCGATGAAGAGAACGTCGCT+TGG | 0.466322 | 4:-81512581 | None:intergenic |
| AGAGAATGGAGTAACCGATT+AGG | 0.466707 | 4:-81512346 | None:intergenic |
| CGGCGGGAGTGCCGTGAAAG+AGG | 0.471269 | 4:-81512421 | None:intergenic |
| GAGGGAAGCGAAGCCGCTGT+TGG | 0.475399 | 4:-81512477 | None:intergenic |
| CCAGCGCTTGTCAGAATACA+TGG | 0.481080 | 4:+81512702 | MsG0480023154.01.T01:CDS |
| TGCCGTCACGTCGTTTCTCA+TGG | 0.494565 | 4:+81512638 | MsG0480023154.01.T01:CDS |
| AAAGAGGGAGATTAAACAGC+TGG | 0.500050 | 4:-81512405 | None:intergenic |
| TTTGTGGGTATACACTCGTT+GGG | 0.509457 | 4:+81512952 | MsG0480023154.01.T01:CDS |
| GATTTGGGTTTCTTGGGCTG+TGG | 0.524093 | 4:+81512901 | MsG0480023154.01.T01:CDS |
| TTTCTGGAAAGCGGTGTTGA+GGG | 0.525576 | 4:-81512495 | None:intergenic |
| AGCGAAGCCGCTGTTGGGGT+CGG | 0.528984 | 4:-81512471 | None:intergenic |
| ACGTGTTGTATTCTATTGTG+AGG | 0.538921 | 4:+81512798 | MsG0480023154.01.T01:CDS |
| GGAAAATCGCTTCGCCGCTA+GGG | 0.544751 | 4:+81512748 | MsG0480023154.01.T01:CDS |
| GGAAAACTAACAAATCTTGG+TGG | 0.550069 | 4:-81512295 | None:intergenic |
| CAGAATACATGGACGCTCAC+CGG | 0.556900 | 4:+81512713 | MsG0480023154.01.T01:CDS |
| ATGGCGCCGAAGATTGCGGC+GGG | 0.560242 | 4:-81512437 | None:intergenic |
| CCATGTATTCTGACAAGCGC+TGG | 0.569670 | 4:-81512702 | None:intergenic |
| GGAGATGGCGCCGAAGATTG+CGG | 0.579772 | 4:-81512441 | None:intergenic |
| GGCGGGAGTGCCGTGAAAGA+GGG | 0.580111 | 4:-81512420 | None:intergenic |
| CGCCATGAGAAACGACGTGA+CGG | 0.582635 | 4:-81512640 | None:intergenic |
| CTTATGGGCTTGTGCCTACG+TGG | 0.601583 | 4:+81512879 | MsG0480023154.01.T01:CDS |
| GGGAAGCGAAGCCGCTGTTG+GGG | 0.604760 | 4:-81512475 | None:intergenic |
| GTGTTGTATTCTATTGTGAG+GGG | 0.606630 | 4:+81512800 | MsG0480023154.01.T01:CDS |
| AACCACGTAGTGTAATAGAT+CGG | 0.612541 | 4:-81512552 | None:intergenic |
| GCTCACCGGTGCTTGCCGGA+AGG | 0.618449 | 4:+81512727 | MsG0480023154.01.T01:CDS |
| AGCGATGCTGTAATCAAGAA+CGG | 0.627277 | 4:-81512519 | None:intergenic |
| AACCGATTAGGTAAATTGCA+AGG | 0.630127 | 4:-81512334 | None:intergenic |
| GAGAACAAGCAAAACGACGA+CGG | 0.631112 | 4:-81512669 | None:intergenic |
| ATCTTCTCCGACCCCAACAG+CGG | 0.637267 | 4:+81512464 | MsG0480023154.01.T01:CDS |
| GGGGTCGGAGAAGATGGAGA+TGG | 0.642155 | 4:-81512456 | None:intergenic |
| AACAGCTGGAGAATTCGGAT+CGG | 0.642303 | 4:-81512391 | None:intergenic |
| GATGGCGCCGAAGATTGCGG+CGG | 0.664185 | 4:-81512438 | None:intergenic |
| AACCGATCTATTACACTACG+TGG | 0.682500 | 4:+81512550 | MsG0480023154.01.T01:CDS |
| CAAGAAACCCAAATCCACGT+AGG | 0.695620 | 4:-81512893 | None:intergenic |
| CGTGACGATAACGAAAAGCG+TGG | 0.719084 | 4:-81512615 | None:intergenic |
| CAGAACATCGTAAACCCTAG+CGG | 0.744153 | 4:-81512762 | None:intergenic |
| GAATACAACACGTAAAACGG+CGG | 0.746815 | 4:-81512788 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAGAATACAACACGTAAAA+CGG | - | Chr4:81512794-81512813 | None:intergenic | 25.0% |
| !!! | TATTGGTGTTAGTATTTTGT+GGG | + | Chr4:81512937-81512956 | MsG0480023154.01.T01:CDS | 25.0% |
| ACCATTCTAAACACAAAGTA+TGG | - | Chr4:81512836-81512855 | None:intergenic | 30.0% | |
| ATGGGAAAACTAACAAATCT+TGG | - | Chr4:81512301-81512320 | None:intergenic | 30.0% | |
| CAAGGAAGAAGAAGAAAAAT+GGG | - | Chr4:81512319-81512338 | None:intergenic | 30.0% | |
| TCCATACTTTGTGTTTAGAA+TGG | + | Chr4:81512832-81512851 | MsG0480023154.01.T01:CDS | 30.0% | |
| TTCCTTGCAATTTACCTAAT+CGG | + | Chr4:81512332-81512351 | MsG0480023154.01.T01:CDS | 30.0% | |
| !!! | CTATTGGTGTTAGTATTTTG+TGG | + | Chr4:81512936-81512955 | MsG0480023154.01.T01:CDS | 30.0% |
| !!! | GGATTTTAGGGTTTTTGTTA+CGG | - | Chr4:81512373-81512392 | None:intergenic | 30.0% |
| AACCACGTAGTGTAATAGAT+CGG | - | Chr4:81512555-81512574 | None:intergenic | 35.0% | |
| AACCGATTAGGTAAATTGCA+AGG | - | Chr4:81512337-81512356 | None:intergenic | 35.0% | |
| AAGAACTGGTGCAAAATTAG+AGG | + | Chr4:81512994-81513013 | MsG0480023154.01.T01:CDS | 35.0% | |
| ACGTGTTGTATTCTATTGTG+AGG | + | Chr4:81512798-81512817 | MsG0480023154.01.T01:CDS | 35.0% | |
| CGTGTTGTATTCTATTGTGA+GGG | + | Chr4:81512799-81512818 | MsG0480023154.01.T01:CDS | 35.0% | |
| CTCATTAGTGAAAGAAGAAC+TGG | + | Chr4:81512980-81512999 | MsG0480023154.01.T01:CDS | 35.0% | |
| GATTAAACAGCTGGAGAATT+CGG | - | Chr4:81512399-81512418 | None:intergenic | 35.0% | |
| GCAAGGAAGAAGAAGAAAAA+TGG | - | Chr4:81512320-81512339 | None:intergenic | 35.0% | |
| GGAAAACTAACAAATCTTGG+TGG | - | Chr4:81512298-81512317 | None:intergenic | 35.0% | |
| GTGTTGTATTCTATTGTGAG+GGG | + | Chr4:81512800-81512819 | MsG0480023154.01.T01:CDS | 35.0% | |
| TGTTTAATCTCCCTCTTTCA+CGG | + | Chr4:81512410-81512429 | MsG0480023154.01.T01:CDS | 35.0% | |
| ! | AGAATTCGGATCGGATTTTA+GGG | - | Chr4:81512385-81512404 | None:intergenic | 35.0% |
| ! | GTTTTTGTTACGGAAGAGAA+TGG | - | Chr4:81512363-81512382 | None:intergenic | 35.0% |
| ! | TGTAATCAAGAACGGTTTTC+TGG | - | Chr4:81512514-81512533 | None:intergenic | 35.0% |
| !!! | ATGGTTTTGTTCTATGCTAG+CGG | + | Chr4:81512851-81512870 | MsG0480023154.01.T01:CDS | 35.0% |
| AAAGAGGGAGATTAAACAGC+TGG | - | Chr4:81512408-81512427 | None:intergenic | 40.0% | |
| AACCGATCTATTACACTACG+TGG | + | Chr4:81512550-81512569 | MsG0480023154.01.T01:CDS | 40.0% | |
| AGAGAATGGAGTAACCGATT+AGG | - | Chr4:81512349-81512368 | None:intergenic | 40.0% | |
| AGCGATGCTGTAATCAAGAA+CGG | - | Chr4:81512522-81512541 | None:intergenic | 40.0% | |
| GAATACAACACGTAAAACGG+CGG | - | Chr4:81512791-81512810 | None:intergenic | 40.0% | |
| TCTATTGTGAGGGGATTTGT+GGG | + | Chr4:81512809-81512828 | MsG0480023154.01.T01:CDS | 40.0% | |
| TTCTATTGTGAGGGGATTTG+TGG | + | Chr4:81512808-81512827 | MsG0480023154.01.T01:CDS | 40.0% | |
| TTTGTGGGTATACACTCGTT+GGG | + | Chr4:81512952-81512971 | MsG0480023154.01.T01:CDS | 40.0% | |
| ! | GAGAATTCGGATCGGATTTT+AGG | - | Chr4:81512386-81512405 | None:intergenic | 40.0% |
| ! | GTGGTGGTTATTTCAGCTAT+TGG | + | Chr4:81512920-81512939 | MsG0480023154.01.T01:CDS | 40.0% |
| ! | TACGTGGATTTGGGTTTCTT+GGG | + | Chr4:81512895-81512914 | MsG0480023154.01.T01:CDS | 40.0% |
| ! | TTTTGTGGGTATACACTCGT+TGG | + | Chr4:81512951-81512970 | MsG0480023154.01.T01:CDS | 40.0% |
| !! | AAGAACGGTTTTCTGGAAAG+CGG | - | Chr4:81512507-81512526 | None:intergenic | 40.0% |
| !!! | TGGTTTTGTTCTATGCTAGC+GGG | + | Chr4:81512852-81512871 | MsG0480023154.01.T01:CDS | 40.0% |
| AACAGCTGGAGAATTCGGAT+CGG | - | Chr4:81512394-81512413 | None:intergenic | 45.0% | |
| CAAGAAACCCAAATCCACGT+AGG | - | Chr4:81512896-81512915 | None:intergenic | 45.0% | |
| CAGAACATCGTAAACCCTAG+CGG | - | Chr4:81512765-81512784 | None:intergenic | 45.0% | |
| GAGAACAAGCAAAACGACGA+CGG | - | Chr4:81512672-81512691 | None:intergenic | 45.0% | |
| ! | CTACGTGGATTTGGGTTTCT+TGG | + | Chr4:81512894-81512913 | MsG0480023154.01.T01:CDS | 45.0% |
| ! | TTTCTGGAAAGCGGTGTTGA+GGG | - | Chr4:81512498-81512517 | None:intergenic | 45.0% |
| !! | TTTTCTGGAAAGCGGTGTTG+AGG | - | Chr4:81512499-81512518 | None:intergenic | 45.0% |
| CAGAATACATGGACGCTCAC+CGG | + | Chr4:81512713-81512732 | MsG0480023154.01.T01:CDS | 50.0% | |
| CCAGCGCTTGTCAGAATACA+TGG | + | Chr4:81512702-81512721 | MsG0480023154.01.T01:CDS | 50.0% | |
| CCATGTATTCTGACAAGCGC+TGG | - | Chr4:81512705-81512724 | None:intergenic | 50.0% | |
| CGATGAAGAGAACGTCGCTT+GGG | - | Chr4:81512583-81512602 | None:intergenic | 50.0% | |
| CGTGACGATAACGAAAAGCG+TGG | - | Chr4:81512618-81512637 | None:intergenic | 50.0% | |
| GCTTGTGCCTACGTGGATTT+GGG | + | Chr4:81512886-81512905 | MsG0480023154.01.T01:CDS | 50.0% | |
| ! | ATTTTCCTTCCGGCAAGCAC+CGG | - | Chr4:81512735-81512754 | None:intergenic | 50.0% |
| ! | GATTTGGGTTTCTTGGGCTG+TGG | + | Chr4:81512901-81512920 | MsG0480023154.01.T01:CDS | 50.0% |
| !! | ATGCTAGCGGGCTTGCTTAT+GGG | + | Chr4:81512864-81512883 | MsG0480023154.01.T01:CDS | 50.0% |
| !! | TATGCTAGCGGGCTTGCTTA+TGG | + | Chr4:81512863-81512882 | MsG0480023154.01.T01:CDS | 50.0% |
| AGGAAAATCGCTTCGCCGCT+AGG | + | Chr4:81512747-81512766 | MsG0480023154.01.T01:CDS | 55.0% | |
| ATCTTCTCCGACCCCAACAG+CGG | + | Chr4:81512464-81512483 | MsG0480023154.01.T01:CDS | 55.0% | |
| CGCCATGAGAAACGACGTGA+CGG | - | Chr4:81512643-81512662 | None:intergenic | 55.0% | |
| CTTATGGGCTTGTGCCTACG+TGG | + | Chr4:81512879-81512898 | MsG0480023154.01.T01:CDS | 55.0% | |
| GCGATGAAGAGAACGTCGCT+TGG | - | Chr4:81512584-81512603 | None:intergenic | 55.0% | |
| GGAAAATCGCTTCGCCGCTA+GGG | + | Chr4:81512748-81512767 | MsG0480023154.01.T01:CDS | 55.0% | |
| GGCTTGTGCCTACGTGGATT+TGG | + | Chr4:81512885-81512904 | MsG0480023154.01.T01:CDS | 55.0% | |
| TGCCGTCACGTCGTTTCTCA+TGG | + | Chr4:81512638-81512657 | MsG0480023154.01.T01:CDS | 55.0% | |
| TTGGGTTTCTTGGGCTGTGG+TGG | + | Chr4:81512904-81512923 | MsG0480023154.01.T01:CDS | 55.0% | |
| ! | CGGCGAAGCGATTTTCCTTC+CGG | - | Chr4:81512745-81512764 | None:intergenic | 55.0% |
| AGGGAAGCGAAGCCGCTGTT+GGG | - | Chr4:81512479-81512498 | None:intergenic | 60.0% | |
| GCTGTTGGGGTCGGAGAAGA+TGG | - | Chr4:81512465-81512484 | None:intergenic | 60.0% | |
| GGGGTCGGAGAAGATGGAGA+TGG | - | Chr4:81512459-81512478 | None:intergenic | 60.0% | |
| ! | GGAGATGGCGCCGAAGATTG+CGG | - | Chr4:81512444-81512463 | None:intergenic | 60.0% |
| AGCGAAGCCGCTGTTGGGGT+CGG | - | Chr4:81512474-81512493 | None:intergenic | 65.0% | |
| GAGGGAAGCGAAGCCGCTGT+TGG | - | Chr4:81512480-81512499 | None:intergenic | 65.0% | |
| GGCACTCCCGCCGCAATCTT+CGG | + | Chr4:81512431-81512450 | MsG0480023154.01.T01:CDS | 65.0% | |
| GGGAAGCGAAGCCGCTGTTG+GGG | - | Chr4:81512478-81512497 | None:intergenic | 65.0% | |
| ! | ATGGCGCCGAAGATTGCGGC+GGG | - | Chr4:81512440-81512459 | None:intergenic | 65.0% |
| ! | GATGGCGCCGAAGATTGCGG+CGG | - | Chr4:81512441-81512460 | None:intergenic | 65.0% |
| !! | GGCGGGAGTGCCGTGAAAGA+GGG | - | Chr4:81512423-81512442 | None:intergenic | 65.0% |
| GCTCACCGGTGCTTGCCGGA+AGG | + | Chr4:81512727-81512746 | MsG0480023154.01.T01:CDS | 70.0% | |
| GGACGCTCACCGGTGCTTGC+CGG | + | Chr4:81512723-81512742 | MsG0480023154.01.T01:CDS | 70.0% | |
| !! | CGGCGGGAGTGCCGTGAAAG+AGG | - | Chr4:81512424-81512443 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 81512281 | 81513030 | 81512281 | ID=MsG0480023154.01;Name=MsG0480023154.01 |
| Chr4 | mRNA | 81512281 | 81513030 | 81512281 | ID=MsG0480023154.01.T01;Parent=MsG0480023154.01;Name=MsG0480023154.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|249 |
| Chr4 | exon | 81512281 | 81513030 | 81512281 | ID=MsG0480023154.01.T01:exon:5533;Parent=MsG0480023154.01.T01 |
| Chr4 | CDS | 81512281 | 81513030 | 81512281 | ID=MsG0480023154.01.T01:cds;Parent=MsG0480023154.01.T01 |
| Gene Sequence |
| Protein sequence |
