AlfalfaGEDB | MsG0480023497.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023497.01

KEGG: -
GO: GO:0003677 GO:0006355
PFAM: PF02365

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023497.01.T01	AER45737.2	96.928	293	3	1	1	287	43	335	0	594

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023497.01.T01	Q39013	62.759	290	102	5	1	287	1	287	8.75E-119	345

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023497.01.T01	G8G271	96.928	293	3	1	1	287	43	335	0.0	594

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TFs/TRs:

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Gene ID	Type	Classification
MsG0480023497.01.T01	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380012400.01	MsG0480023497.01	0.800540	1.373983e-48	6.414035e-46

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023497.01.T01	MTR_8g094580	91.809	293	10	5	1	287	1	285	0.0	538
MsG0480023497.01.T01	MTR_8g094580	89.958	239	10	5	55	287	1	231	9.34e-152	424
MsG0480023497.01.T01	MTR_3g096920	69.024	297	69	7	1	284	1	287	3.36e-134	382
MsG0480023497.01.T01	MTR_3g096920	61.261	333	71	7	1	284	1	324	4.00e-128	367
MsG0480023497.01.T01	MTR_3g088110	63.087	298	87	7	1	287	1	286	5.80e-120	345
MsG0480023497.01.T01	MTR_2157s0010	66.258	163	46	3	7	160	8	170	1.90e-75	231
MsG0480023497.01.T01	MTR_2g079990	67.702	161	47	2	5	164	14	170	3.36e-75	234
MsG0480023497.01.T01	MTR_2g080010	67.683	164	39	3	7	158	15	176	4.75e-75	233
MsG0480023497.01.T01	MTR_7g085220	54.589	207	78	3	7	198	8	213	1.26e-73	229
MsG0480023497.01.T01	MTR_8g059170	68.944	161	47	2	5	164	12	170	6.55e-73	227

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023497.01.T01	AT1G01720	62.759	290	102	5	1	287	1	287	8.92e-120	345
MsG0480023497.01.T01	AT5G08790	63.542	288	76	8	1	280	1	267	1.20e-116	337
MsG0480023497.01.T01	AT5G63790	60.473	296	70	9	1	286	44	302	1.76e-109	320
MsG0480023497.01.T01	AT5G63790	60.959	292	67	9	1	282	54	308	2.00e-109	320
MsG0480023497.01.T01	AT1G77450	60.153	261	59	7	5	262	8	226	3.79e-96	283
MsG0480023497.01.T01	AT1G69490	54.369	206	88	3	7	211	9	209	8.40e-76	232
MsG0480023497.01.T01	AT1G52890	67.702	161	47	2	5	164	12	168	2.24e-74	230
MsG0480023497.01.T01	AT3G15500	66.867	166	48	3	5	167	12	173	6.47e-74	229
MsG0480023497.01.T01	AT4G27410	69.677	155	42	2	5	158	12	162	1.49e-73	228
MsG0480023497.01.T01	AT1G61110	65.663	166	47	2	3	158	12	177	1.24e-71	224

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Find 122 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGAAAATGACCATTT+GGG	0.367814	4:+86044993	MsG0480023497.01.T01:three_prime_UTR
AAACCTCAGATCAAGATGAA+CGG	0.656051	4:+86044624	MsG0480023497.01.T01:CDS
AAACGGTTCAAGGCCGAACC+GGG	0.528831	4:+86044191	MsG0480023497.01.T01:CDS
AAATCAATTTCTTTGATAAT+AGG	0.219498	4:-86043987	None:intergenic
AACCAATTTAAAATATCCAT+GGG	0.565157	4:-86045044	None:intergenic
AACCCGAAAATTGATTCATT+TGG	0.363530	4:-86044884	None:intergenic
AACGAGGTGTAGATTCAATT+AGG	0.431824	4:+86045283	MsG0480023497.01.T01:three_prime_UTR
AAGATTCAACTGAAAATCAA+AGG	0.566776	4:-86044817	None:intergenic
AAGTGGAATGAATTTGTATT+GGG	0.373773	4:+86044777	MsG0480023497.01.T01:CDS
AAGTTCAAAGTGAGCCCAAG+TGG	0.609329	4:+86044760	MsG0480023497.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTTGCAGTTAGTATTTGTT+TGG	-	Chr4:86045100-86045119	None:intergenic	30.0%
ATAGAAATAGGTCATGATCA+TGG	+	Chr4:86044964-86044983	MsG0480023497.01.T01:three_prime_UTR	30.0%
ATGTACGTACTCTTAAATTG+TGG	-	Chr4:86044398-86044417	None:intergenic	30.0%
CAAATTAAATTTACTTGGGC+AGG	-	Chr4:86044929-86044948	None:intergenic	30.0%
CAAGTGGAATGAATTTGTAT+TGG	+	Chr4:86044776-86044795	MsG0480023497.01.T01:CDS	30.0%
CAATACAAATTCATTCCACT+TGG	-	Chr4:86044778-86044797	None:intergenic	30.0%
CCAAAGGAATTAAAACCAAT+TGG	+	Chr4:86044319-86044338	MsG0480023497.01.T01:CDS	30.0%
GAAAATAAAGAGTAAGTACC+TGG	-	Chr4:86044038-86044057	None:intergenic	30.0%
GTCGAATTTACAACAAGAAA+GGG	+	Chr4:86044532-86044551	MsG0480023497.01.T01:CDS	30.0%
TAGAAATAGGTCATGATCAT+GGG	+	Chr4:86044965-86044984	MsG0480023497.01.T01:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	86043754	86045430	86043754	ID=MsG0480023497.01;Name=MsG0480023497.01
Chr4	mRNA	86043754	86045430	86043754	ID=MsG0480023497.01.T01;Parent=MsG0480023497.01;Name=MsG0480023497.01.T01;_AED=0.08;_eAED=0.08;_QI=125\|1\|1\|1\|1\|1\|3\|492\|287
Chr4	exon	86043754	86044038	86043754	ID=MsG0480023497.01.T01:exon:2940;Parent=MsG0480023497.01.T01
Chr4	exon	86044130	86044407	86044130	ID=MsG0480023497.01.T01:exon:2941;Parent=MsG0480023497.01.T01
Chr4	exon	86044513	86045430	86044513	ID=MsG0480023497.01.T01:exon:2942;Parent=MsG0480023497.01.T01
Chr4	five_prime_UTR	86043754	86043878	86043754	ID=MsG0480023497.01.T01:five_prime_utr;Parent=MsG0480023497.01.T01
Chr4	CDS	86043879	86044038	86043879	ID=MsG0480023497.01.T01:cds;Parent=MsG0480023497.01.T01
Chr4	CDS	86044130	86044407	86044130	ID=MsG0480023497.01.T01:cds;Parent=MsG0480023497.01.T01
Chr4	CDS	86044513	86044938	86044513	ID=MsG0480023497.01.T01:cds;Parent=MsG0480023497.01.T01
Chr4	three_prime_UTR	86044939	86045430	86044939	ID=MsG0480023497.01.T01:three_prime_utr;Parent=MsG0480023497.01.T01

Gene Sequence

>MsG0480023497.01.T01

ATGCAAGGAGCATTAGAATTACCACCTGGCTTCAGATTTCACCCGACTGATGATGAGCTTGTCAATCACTACTTGTGTAGAAAGTGTGCTTCCCTCCCTATAGCTGTTCCTATTATCAAAGAAATTGATTTGTATAAGTTTGATCCATGGCATCTTCCAGAAATGGCTCTTTACGGTGAGAAAGAGTGGTATTTTTTCTCTCCAAGGGATCGTAAATATCCAAACGGTTCAAGGCCGAACCGGGCTGCGGGAACCGGATATTGGAAGGCGACCGGAGCTGATAAGCCGATTGGAAAGCCGAAGGCACTTGGGATAAAGAAAGCACTTGTTTTCTATGCTGGCAAAGCTCCCAAAGGAATTAAAACCAATTGGATCATGCATGAGTATCGTCTTGCTAATGTTGATAGATCCGCTTCAAAAAATAACCACAATTTAAGACTTGATGATTGGGTCTTGTGTCGAATTTACAACAAGAAAGGGAAGATTGAGAAATACAATGACACTAAACCACCACTGTTTTCTGAGGAAGCTCAATTTGAGAATGAGACAAAACCTCAGATCAAGATGAACGGCCAGGATGATTTTAGGAATAGTGATCAATTGTACACTGACACATCAGATTCGGTGCCACGGTTTCACACCGACTCAAGCGGTTCTGAGCACGTGGTTTCACCGTGCGATAAGGAAGTTCAAAGTGAGCCCAAGTGGAATGAATTTGTATTGGGCCCAGACCCAGTTTCAGCCTTTGATTTTCAGTTGAATCTTATGGATGATGGTGAAGATGACCCTTTTGCCCCTCAGGATCAGTACCAAATGAATCAATTTTCGGGTTGGCAAGATGTGTTTATGTACCTGCCCAAGTAA

Protein sequence

>MsG0480023497.01.T01

MQGALELPPGFRFHPTDDELVNHYLCRKCASLPIAVPIIKEIDLYKFDPWHLPEMALYGEKEWYFFSPRDRKYPNGSRPNRAAGTGYWKATGADKPIGKPKALGIKKALVFYAGKAPKGIKTNWIMHEYRLANVDRSASKNNHNLRLDDWVLCRIYNKKGKIEKYNDTKPPLFSEEAQFENETKPQIKMNGQDDFRNSDQLYTDTSDSVPRFHTDSSGSEHVVSPCDKEVQSEPKWNEFVLGPDPVSAFDFQLNLMDDGEDDPFAPQDQYQMNQFSGWQDVFMYLPK*