AlfalfaGEDB | MsG0480023691.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023691.01

KEGG: -
GO: GO:0000723 GO:0003678 GO:0006281
PFAM: PF05970

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023691.01.T01	XP_039683757.1	88.462	442	37	1	1	442	368	795	0	796

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023691.01.T01	A0A072TWN2	79.412	442	46	2	1	442	149	545	0.0	711
MsG0480023691.01.T01	G7I6F3	80.278	431	60	2	1	431	16	421	0.0	711

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480023691.01	MsG0480023970.01	0.824122	9.577579e-54	8.407150e-51

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0480023691.01.T01	MTR_7g022020	79.412	442	46	2	1	442	149	545	711
MsG0480023691.01.T01	MTR_1g012040	80.278	431	60	2	1	431	16	421	711
MsG0480023691.01.T01	MTR_1g021210	79.350	431	59	3	4	431	194	597	682
MsG0480023691.01.T01	MTR_5g061500	73.799	458	91	5	1	457	16	445	654
MsG0480023691.01.T01	MTR_4g120080	78.102	411	60	3	1	411	350	730	636
MsG0480023691.01.T01	MTR_2g064230	80.051	391	57	1	51	441	1	370	633
MsG0480023691.01.T01	MTR_2g066670	77.723	404	62	3	38	441	1	376	631
MsG0480023691.01.T01	MTR_2g089470	74.769	432	66	5	1	426	355	749	619
MsG0480023691.01.T01	MTR_2g068990	74.769	432	66	5	1	426	355	749	619
MsG0480023691.01.T01	MTR_3g034020	69.683	442	50	2	1	442	16	373	600

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023691.01.T01	AT3G51690	35.354	198	124	2	245	442	59	252	1.03e-34	133
MsG0480023691.01.T01	AT5G28780	33.784	222	137	3	251	463	62	282	7.94e-33	128
MsG0480023691.01.T01	AT3G51700	36.318	201	121	3	245	442	60	256	7.08e-32	125
MsG0480023691.01.T01	AT4G23885	50.909	55	27	0	465	519	23	77	9.82e-11	58.5

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Find 135 sgRNAs with CRISPR-Local

Find 327 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGCTTAAGTGCGTTTA+GGG	0.302182	4:+88619482	None:intergenic
AAACACCATCGGTGCATCAT+TGG	0.407286	4:+88619552	None:intergenic
AAAGGCTTAAGTGCGTTTAG+GGG	0.650964	4:+88619483	None:intergenic
AAAGTTGATAATTTGGGATG+AGG	0.530650	4:-88619577	MsG0480023691.01.T01:CDS
AAATACTCTTTCTCTTCACC+TGG	0.504876	4:+88619015	None:intergenic
AAATCACCGGTTGAAACTAA+AGG	0.539950	4:-88618893	MsG0480023691.01.T01:CDS
AACGATGCTTCGGATGAGAA+TGG	0.551038	4:-88619231	MsG0480023691.01.T01:CDS
AACTGCATCAATCTCCTCAT+TGG	0.450632	4:+88615953	None:intergenic
AACTTTGATTAATCAGAGCC+AGG	0.454568	4:+88615334	None:intergenic
AAGAACTCCGGTGACCAATG+AGG	0.662672	4:-88615967	MsG0480023691.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTCTATAAGTGACATCAA+CGG	+	Chr4:88616003-88616022	None:intergenic	30.0%
AGAGCCTCATAATATAACAT+TGG	-	Chr4:88618873-88618892	MsG0480023691.01.T01:CDS	30.0%
AGGTTAAGTAAGTATTCATG+AGG	-	Chr4:88618100-88618119	MsG0480023691.01.T01:intron	30.0%
AGTAAGGAGAATAGATCAAT+AGG	-	Chr4:88618429-88618448	MsG0480023691.01.T01:intron	30.0%
AGTCAATTCGTCAAAGTTAT+GGG	-	Chr4:88615786-88615805	MsG0480023691.01.T01:intron	30.0%
ATGCCTCATTAATCTCAATA+GGG	+	Chr4:88615507-88615526	None:intergenic	30.0%
ATGTTTCTCATCAGCATAAT+CGG	+	Chr4:88616297-88616316	None:intergenic	30.0%
CATTGATGTAAAAGGAATTG+AGG	+	Chr4:88618060-88618079	None:intergenic	30.0%
CTCAAGCAACAAAAAGAAAA+AGG	+	Chr4:88619862-88619881	None:intergenic	30.0%
CTCATAAAACAACACATTTC+TGG	-	Chr4:88619296-88619315	MsG0480023691.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	88615160	88620018	88615160	ID=MsG0480023691.01;Name=MsG0480023691.01
Chr4	mRNA	88615160	88620018	88615160	ID=MsG0480023691.01.T01;Parent=MsG0480023691.01;Name=MsG0480023691.01.T01;_AED=0.38;_eAED=0.40;_QI=0\|0.33\|0.5\|1\|0.66\|0.5\|4\|235\|519
Chr4	exon	88615160	88615412	88615160	ID=MsG0480023691.01.T01:exon:7630;Parent=MsG0480023691.01.T01
Chr4	exon	88615950	88616101	88615950	ID=MsG0480023691.01.T01:exon:7629;Parent=MsG0480023691.01.T01
Chr4	exon	88616238	88616303	88616238	ID=MsG0480023691.01.T01:exon:7628;Parent=MsG0480023691.01.T01
Chr4	exon	88618695	88620018	88618695	ID=MsG0480023691.01.T01:exon:7627;Parent=MsG0480023691.01.T01
Chr4	CDS	88618695	88620018	88618695	ID=MsG0480023691.01.T01:cds;Parent=MsG0480023691.01.T01
Chr4	CDS	88616238	88616303	88616238	ID=MsG0480023691.01.T01:cds;Parent=MsG0480023691.01.T01
Chr4	CDS	88615950	88616101	88615950	ID=MsG0480023691.01.T01:cds;Parent=MsG0480023691.01.T01
Chr4	CDS	88615395	88615412	88615395	ID=MsG0480023691.01.T01:cds;Parent=MsG0480023691.01.T01
Chr4	three_prime_UTR	88615160	88615394	88615160	ID=MsG0480023691.01.T01:three_prime_utr;Parent=MsG0480023691.01.T01

Gene Sequence

>MsG0480023691.01.T01

ATGCCAAGGCCAAACGCAGCAGACATGCCAACTTTCACAAACAAGCTTATCGTTGATGAGCTAAGTTACAACAAAGATGAGCTGGAAAAGACACACGCTGATATGTTACTTATGCTGACTGATGAACAAAGATGTGTGCATGACAAGATCATGGAGTCCGTTGGTTCTGACGACGGTGGTTTATTTTTCTTATATGGTTACGGTGGTACTGGAAAAACCTTTATATGGAAAACATTGTCGGCTGCTGTTAGATCGAAGGGGTTAATTGTATTGAATGTTGCATCCAGCGGTATAGCGGCTCTTCTATTACCAGGTGGAAGAACCGCGCACTCGACGCTGACAGTCCCTATTGAGATTAATGAGGCATCATCGCTTACGATGGAAAAGGACAGTCCTAGGGCAGAACTTGTGCGTGCTGCAAAGTTGATAATTTGGGATGAGGCTCCAATGATGCACCGATGGTGTTTCGAGGCAGTTGACCGTTCATTGCGTGATATCATGTTCAAGAATGATCCCCTAAACGCACTTAAGCCTTTTGGTGGAATGACATTAGTTTTGGGTGGTGATTTCAAGCAGATATTGCCTATTATCCGAGGGGCAACGAGGCCAGATATTGTTGATGCCTTAGTCAATTCGTCAAAGTTATGGGCTTATTGTAATGTGTTGAGGCTTACTGTTAATATGAGATTGGGTGCATCTTCGGTTCTTGCAGAGCAGGAAGAAATTGCTAATTTTGGCAAGTTGATACTCTCGATTGGAGACGGCAACGATGCTTCGGATGAGAATGGTGAAATGAAGGTAGAAATACCTGAAGATTTGCTGATATCGGACACAACAAATCCGTTGATGTCACTTATAGACTTTGTGTATCCCGATCTGAATGATAACCTTGGCGACCAGCTATTTTTCCAAGAAAGGGGAATACTCGCACCGACGCTTGATTCAGTTCAACATGTTAACGAATATATGATGTCGCTGATTCCAGGTGAAGAGAAAGAGTATTTAAGCTCTGATTCTGTTTGTCGATCGGGTGAAAATTCTGATGTCCAAAGCGAGTGGTTCACAACCGAATTTCTTAATGGTATTAACAACTCTGGAATTCCAAATCACCGGTTGAAACTAAAGGTGGGATGTCCGATTATGCTGATGAGAAACATCGATCAGACCAATGGACTGTGTAATGGTACGAGGCTGACAGTTACACATCTAGGGAAGAGCACGATAGCTGCTACCGTAATTACAGGAAAAAGGGCAGGTACCAGGGTATTCATTCCCAGGATGAATCTTATTCCGAGTGATCCAGGACTACCCTTCAAATTCAGGCAGCCTCATAATATAACATTGGTTGATCCAAGTGGTCGATCCATTCCACCCAATGAGATGAGGCTTGGTTCTACCTCTGAGGCTCAAGCTTTGTCTTCGAAAAGCGATGGCAGTTTATCATTCTTTTCAAGTTCAAATGCTTCAACAACATTAGGAGGAAAGGCCTCTCTTCAACCCAAAAGAACTCCGGTGACCAATGAGGAGATTGATGCAGTTTTGTTGGGTGGCTGCTTCTAA

Protein sequence

>MsG0480023691.01.T01

MPRPNAADMPTFTNKLIVDELSYNKDELEKTHADMLLMLTDEQRCVHDKIMESVGSDDGGLFFLYGYGGTGKTFIWKTLSAAVRSKGLIVLNVASSGIAALLLPGGRTAHSTLTVPIEINEASSLTMEKDSPRAELVRAAKLIIWDEAPMMHRWCFEAVDRSLRDIMFKNDPLNALKPFGGMTLVLGGDFKQILPIIRGATRPDIVDALVNSSKLWAYCNVLRLTVNMRLGASSVLAEQEEIANFGKLILSIGDGNDASDENGEMKVEIPEDLLISDTTNPLMSLIDFVYPDLNDNLGDQLFFQERGILAPTLDSVQHVNEYMMSLIPGEEKEYLSSDSVCRSGENSDVQSEWFTTEFLNGINNSGIPNHRLKLKVGCPIMLMRNIDQTNGLCNGTRLTVTHLGKSTIAATVITGKRAGTRVFIPRMNLIPSDPGLPFKFRQPHNITLVDPSGRSIPPNEMRLGSTSEAQALSSKSDGSLSFFSSSNASTTLGGKASLQPKRTPVTNEEIDAVLLGGCF*