AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023970.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023970.01.T01 MTR_8g106080 100.000 140 0 0 1 140 1 140 1.22e-100 285
MsG0480023970.01.T01 MTR_8g106020 100.000 140 0 0 1 140 1 140 1.22e-100 285
MsG0480023970.01.T01 MTR_5g075240 99.286 140 1 0 1 140 1 140 2.74e-100 283
MsG0480023970.01.T01 MTR_4g085580 95.000 140 7 0 1 140 4 143 3.03e-96 273
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023970.01.T01 AT2G09990 89.928 139 14 0 2 140 8 146 5.38e-92 263
MsG0480023970.01.T01 AT5G18380 89.209 139 15 0 2 140 8 146 9.20e-92 262
MsG0480023970.01.T01 AT3G04230 84.173 139 22 0 2 140 8 146 1.91e-85 246
MsG0480023970.01.T01 AT5G18380 87.200 125 16 0 2 126 8 132 3.83e-79 230
MsG0480023970.01.T01 AT5G18380 87.037 108 14 0 2 109 8 115 1.18e-67 201

Find 38 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCTAACGATAAGATTTC+TGG 0.210396 4:+91797773 None:intergenic
ACAGAGTAAGAAGGAGATTA+AGG 0.258952 4:-91797890 MsG0480023970.01.T01:CDS
CGCTGTGAGCCTAAGAAGTT+TGG 0.359075 4:-91797820 MsG0480023970.01.T01:CDS
CTGCAACAGCGTTCTTCTTC+CGG 0.375769 4:+91798154 None:intergenic
TCAGAGTATTGCGAAGGCTT+TGG 0.400647 4:-91797941 MsG0480023970.01.T01:CDS
TACGAACCAATCCTCCTTCT+CGG 0.413918 4:-91798042 MsG0480023970.01.T01:CDS
CCACGACCACCAAACTTCTT+AGG 0.436009 4:+91797811 None:intergenic
ATGGAACAGGTGCAATGCTT+TGG 0.453443 4:-91798177 MsG0480023970.01.T01:CDS
GAAGCTCAGCAAGATGGAAC+AGG 0.488449 4:-91798190 None:intergenic
CAATGGCTGTCCAATCGAAC+TGG 0.489439 4:-91798094 MsG0480023970.01.T01:CDS
CGCGGTCTCATCAAGATCAA+TGG 0.494184 4:-91798111 MsG0480023970.01.T01:CDS
TCATATCAACTCCGGCGAAA+CGG 0.494924 4:+91798013 None:intergenic
CTCGGCAGACACCGTTTCGC+CGG 0.496313 4:-91798024 MsG0480023970.01.T01:CDS
TCAGGCTCCACCAGTTCGAT+TGG 0.496656 4:+91798084 None:intergenic
GTGGTCGTGGTGCTCGTGCT+AGG 0.502073 4:-91797798 MsG0480023970.01.T01:CDS
GCTTTGAAACGAAGAATCTC+AGG 0.503475 4:+91798066 None:intergenic
GTTTCGCCGGAGTTGATATG+AGG 0.511168 4:-91798011 MsG0480023970.01.T01:CDS
CCTAAGAAGTTTGGTGGTCG+TGG 0.511400 4:-91797811 MsG0480023970.01.T01:CDS
AGGATCCGTGTGAAGGGTGG+AGG 0.521481 4:-91797991 MsG0480023970.01.T01:CDS
TGTTGATGAACAGAGTAAGA+AGG 0.534508 4:-91797899 MsG0480023970.01.T01:CDS
CGAAACGGTGTCTGCCGAGA+AGG 0.541529 4:+91798028 None:intergenic
AACAGGTGCAATGCTTTGGC+CGG 0.546867 4:-91798173 MsG0480023970.01.T01:CDS
TGTGAGCCTAAGAAGTTTGG+TGG 0.558448 4:-91797817 MsG0480023970.01.T01:CDS
ACCCACTGCAAGAGAGGTCG+CGG 0.562945 4:-91798129 MsG0480023970.01.T01:CDS
AACGGTGTCTGCCGAGAAGG+AGG 0.579454 4:+91798031 None:intergenic
GCAGTTACCCACTGCAAGAG+AGG 0.580125 4:-91798135 MsG0480023970.01.T01:CDS
TGGCTGTCCAATCGAACTGG+TGG 0.587642 4:-91798091 MsG0480023970.01.T01:CDS
TTCTTAGGCTCACAGCGTCT+AGG 0.590494 4:+91797826 None:intergenic
TGTCTGCCGAGAAGGAGGAT+TGG 0.604124 4:+91798036 None:intergenic
CTTCGCAATACTCTGACGAA+TGG 0.610127 4:+91797948 None:intergenic
ACCGCGACCTCTCTTGCAGT+GGG 0.618077 4:+91798128 None:intergenic
CATTCGTCAGAGTATTGCGA+AGG 0.620290 4:-91797947 MsG0480023970.01.T01:CDS
GACCGCGACCTCTCTTGCAG+TGG 0.624398 4:+91798127 None:intergenic
TGATATGAGGATCCGTGTGA+AGG 0.626071 4:-91797998 MsG0480023970.01.T01:CDS
ATGAGGATCCGTGTGAAGGG+TGG 0.634725 4:-91797994 MsG0480023970.01.T01:CDS
TGTGACCTCCACCCTTCACA+CGG 0.645312 4:+91797986 None:intergenic
ACGGATCCTCATATCAACTC+CGG 0.648153 4:+91798005 None:intergenic
GATATGAGGATCCGTGTGAA+GGG 0.662094 4:-91797997 MsG0480023970.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACAGAGTAAGAAGGAGATTA+AGG - Chr4:91798064-91798083 MsG0480023970.01.T01:CDS 35.0%
! TGTTGATGAACAGAGTAAGA+AGG - Chr4:91798055-91798074 MsG0480023970.01.T01:CDS 35.0%
!! GCTTTGAAACGAAGAATCTC+AGG + Chr4:91797891-91797910 None:intergenic 40.0%
ACGGATCCTCATATCAACTC+CGG + Chr4:91797952-91797971 None:intergenic 45.0%
ATGGAACAGGTGCAATGCTT+TGG - Chr4:91797777-91797796 MsG0480023970.01.T01:CDS 45.0%
CTTCGCAATACTCTGACGAA+TGG + Chr4:91798009-91798028 None:intergenic 45.0%
GATATGAGGATCCGTGTGAA+GGG - Chr4:91797957-91797976 MsG0480023970.01.T01:CDS 45.0%
TACGAACCAATCCTCCTTCT+CGG - Chr4:91797912-91797931 MsG0480023970.01.T01:CDS 45.0%
TCATATCAACTCCGGCGAAA+CGG + Chr4:91797944-91797963 None:intergenic 45.0%
TGATATGAGGATCCGTGTGA+AGG - Chr4:91797956-91797975 MsG0480023970.01.T01:CDS 45.0%
TGTGAGCCTAAGAAGTTTGG+TGG - Chr4:91798137-91798156 MsG0480023970.01.T01:CDS 45.0%
!! CATTCGTCAGAGTATTGCGA+AGG - Chr4:91798007-91798026 MsG0480023970.01.T01:CDS 45.0%
!! TCAGAGTATTGCGAAGGCTT+TGG - Chr4:91798013-91798032 MsG0480023970.01.T01:CDS 45.0%
AACAGGTGCAATGCTTTGGC+CGG - Chr4:91797781-91797800 MsG0480023970.01.T01:CDS 50.0%
CAATGGCTGTCCAATCGAAC+TGG - Chr4:91797860-91797879 MsG0480023970.01.T01:CDS 50.0%
CCACGACCACCAAACTTCTT+AGG + Chr4:91798146-91798165 None:intergenic 50.0%
CGCGGTCTCATCAAGATCAA+TGG - Chr4:91797843-91797862 MsG0480023970.01.T01:CDS 50.0%
CGCTGTGAGCCTAAGAAGTT+TGG - Chr4:91798134-91798153 MsG0480023970.01.T01:CDS 50.0%
CTGCAACAGCGTTCTTCTTC+CGG + Chr4:91797803-91797822 None:intergenic 50.0%
TTCTTAGGCTCACAGCGTCT+AGG + Chr4:91798131-91798150 None:intergenic 50.0%
! CCTAAGAAGTTTGGTGGTCG+TGG - Chr4:91798143-91798162 MsG0480023970.01.T01:CDS 50.0%
!! GTTTCGCCGGAGTTGATATG+AGG - Chr4:91797943-91797962 MsG0480023970.01.T01:CDS 50.0%
ATGAGGATCCGTGTGAAGGG+TGG - Chr4:91797960-91797979 MsG0480023970.01.T01:CDS 55.0%
GCAGTTACCCACTGCAAGAG+AGG - Chr4:91797819-91797838 MsG0480023970.01.T01:CDS 55.0%
TCAGGCTCCACCAGTTCGAT+TGG + Chr4:91797873-91797892 None:intergenic 55.0%
TGGCTGTCCAATCGAACTGG+TGG - Chr4:91797863-91797882 MsG0480023970.01.T01:CDS 55.0%
TGTCTGCCGAGAAGGAGGAT+TGG + Chr4:91797921-91797940 None:intergenic 55.0%
TGTGACCTCCACCCTTCACA+CGG + Chr4:91797971-91797990 None:intergenic 55.0%
AACGGTGTCTGCCGAGAAGG+AGG + Chr4:91797926-91797945 None:intergenic 60.0%
ACCCACTGCAAGAGAGGTCG+CGG - Chr4:91797825-91797844 MsG0480023970.01.T01:CDS 60.0%
ACCGCGACCTCTCTTGCAGT+GGG + Chr4:91797829-91797848 None:intergenic 60.0%
AGGATCCGTGTGAAGGGTGG+AGG - Chr4:91797963-91797982 MsG0480023970.01.T01:CDS 60.0%
CGAAACGGTGTCTGCCGAGA+AGG + Chr4:91797929-91797948 None:intergenic 60.0%
CTCGGCAGACACCGTTTCGC+CGG - Chr4:91797930-91797949 MsG0480023970.01.T01:CDS 65.0%
GACCGCGACCTCTCTTGCAG+TGG + Chr4:91797830-91797849 None:intergenic 65.0%
GTGGTCGTGGTGCTCGTGCT+AGG - Chr4:91798156-91798175 MsG0480023970.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 91797777 91798199 91797777 ID=MsG0480023970.01;Name=MsG0480023970.01
Chr4 mRNA 91797777 91798199 91797777 ID=MsG0480023970.01.T01;Parent=MsG0480023970.01;Name=MsG0480023970.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|140
Chr4 exon 91797777 91798199 91797777 ID=MsG0480023970.01.T01:exon:12817;Parent=MsG0480023970.01.T01
Chr4 CDS 91797777 91798199 91797777 ID=MsG0480023970.01.T01:cds;Parent=MsG0480023970.01.T01
Gene Sequence

>MsG0480023970.01.T01

ATGGAACAGGTGCAATGCTTTGGCCGGAAGAAGAACGCTGTTGCAGTTACCCACTGCAAGAGAGGTCGCGGTCTCATCAAGATCAATGGCTGTCCAATCGAACTGGTGGAGCCTGAGATTCTTCGTTTCAAAGCATACGAACCAATCCTCCTTCTCGGCAGACACCGTTTCGCCGGAGTTGATATGAGGATCCGTGTGAAGGGTGGAGGTCACACTTCTCAGATCTATGCCATTCGTCAGAGTATTGCGAAGGCTTTGGTTGCTTATTATCAGAAATATGTTGATGAACAGAGTAAGAAGGAGATTAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATCGTTAG

Protein sequence

>MsG0480023970.01.T01

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKYVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR*