AlfalfaGEDB | MsG0480023827.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023827.01

KEGG: K08235
GO: GO:0004553 GO:0005618 GO:0005975 GO:0006073 GO:0010411 GO:0016762 GO:0042546 GO:0048046
PFAM: PF00722 PF06955

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023827.01.T01	XP_003630750.2	87.869	305	7	2	1	290	1	290	0	550

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023827.01.T01	Q9SVV2	52.492	301	112	3	6	290	7	292	1.70E-111	327

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023827.01.T01	G7L858	87.625	299	7	2	7	290	1	284	0.0	538

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0480023827.01	MsG0880042313.01	PPI
MsG0880046024.01	MsG0480023827.01	PPI
MsG0880046024.01	MsG0480023827.01	PPI
MsG0880046025.01	MsG0480023827.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023827.01.T01	MTR_8g102910	87.625	299	7	2	7	290	1	284	0.0	538
MsG0480023827.01.T01	MTR_6g088320	68.197	305	65	4	1	290	1	288	2.68e-155	435
MsG0480023827.01.T01	MTR_2g038860	44.625	307	134	6	2	289	1	290	1.67e-87	263
MsG0480023827.01.T01	MTR_2g038705	44.625	307	134	6	2	289	1	290	1.67e-87	263
MsG0480023827.01.T01	MTR_4g128410	45.066	304	129	5	2	288	1	283	1.71e-86	260
MsG0480023827.01.T01	MTR_7g093530	46.048	291	121	4	12	285	8	279	4.67e-85	256
MsG0480023827.01.T01	MTR_4g128570	45.270	296	110	6	7	285	12	272	1.04e-83	253
MsG0480023827.01.T01	MTR_4g128580	46.853	286	114	6	20	285	18	285	4.75e-82	249
MsG0480023827.01.T01	MTR_4g128400	45.035	282	119	4	21	285	21	283	4.32e-81	246
MsG0480023827.01.T01	MTR_3g084630	44.118	306	126	7	2	290	5	282	5.58e-81	246

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023827.01.T01	AT4G28850	52.492	301	112	3	6	290	7	292	1.73e-112	327
MsG0480023827.01.T01	AT5G57560	45.638	298	122	8	8	286	6	282	2.14e-85	258
MsG0480023827.01.T01	AT4G25810	44.737	304	128	6	1	286	3	284	5.05e-85	256
MsG0480023827.01.T01	AT5G57530	47.482	278	108	6	26	285	25	282	4.27e-83	252
MsG0480023827.01.T01	AT5G57550	43.813	299	121	6	12	285	6	282	8.15e-82	248
MsG0480023827.01.T01	AT3G23730	45.070	284	120	5	25	289	24	290	1.26e-81	248
MsG0480023827.01.T01	AT2G18800	41.720	314	136	7	1	285	1	296	6.42e-81	247
MsG0480023827.01.T01	AT4G14130	45.230	283	116	5	26	289	26	288	1.63e-79	243
MsG0480023827.01.T01	AT5G57540	45.126	277	116	5	26	285	24	281	2.32e-79	242
MsG0480023827.01.T01	AT4G25820	44.186	301	111	8	9	285	18	285	2.39e-79	242

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Find 62 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCATGTACCTCACCTG+GGG	0.718109	4:-90217212	MsG0480023827.01.T01:CDS
AAAGATTACAAAAGATTCAA+CGG	0.569128	4:-90215743	MsG0480023827.01.T01:CDS
AACCTATTTACACAAGGAAA+AGG	0.421723	4:-90216327	MsG0480023827.01.T01:CDS
AATACTAACCTATTTACACA+AGG	0.711992	4:-90216333	MsG0480023827.01.T01:CDS
AATTGTGAAAATCAGCAGTT+GGG	0.553882	4:+90216271	None:intergenic
ACAACTTACACAATTTCTGT+GGG	0.541852	4:+90216231	None:intergenic
ACCCTCATTCCTTGCTTGTT+TGG	0.311158	4:+90216028	None:intergenic
ACCTATTTACACAAGGAAAA+GGG	0.504561	4:-90216326	MsG0480023827.01.T01:CDS
ACCTTAGTTATGGCATGATA+AGG	0.484940	4:-90215811	MsG0480023827.01.T01:CDS
AGCAATGCACCATTCAAAGT+AGG	0.545062	4:-90215941	MsG0480023827.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACCTATTTACACAAGGAAA+AGG	-	Chr4:90216678-90216697	MsG0480023827.01.T01:intron	30.0%
AAGCAATTAGATGGTAACTT+GGG	+	Chr4:90216861-90216880	None:intergenic	30.0%
AATTGTGAAAATCAGCAGTT+GGG	+	Chr4:90216737-90216756	None:intergenic	30.0%
ACAACTTACACAATTTCTGT+GGG	+	Chr4:90216777-90216796	None:intergenic	30.0%
ACCTATTTACACAAGGAAAA+GGG	-	Chr4:90216679-90216698	MsG0480023827.01.T01:intron	30.0%
ACTGAGAGAAACATATTCAT+TGG	-	Chr4:90216297-90216316	MsG0480023827.01.T01:CDS	30.0%
ATCAAGTGAACTTACATAGT+AGG	+	Chr4:90216466-90216485	None:intergenic	30.0%
ATCATGACTATTATTTCAGG+TGG	-	Chr4:90216894-90216913	MsG0480023827.01.T01:intron	30.0%
ATGTTAATAAAGCTGATACC+TGG	-	Chr4:90216417-90216436	MsG0480023827.01.T01:intron	30.0%
GCTAATCTGGAAATATTACA+TGG	-	Chr4:90216327-90216346	MsG0480023827.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	90215706	90217321	90215706	ID=MsG0480023827.01;Name=MsG0480023827.01
Chr4	mRNA	90215706	90217321	90215706	ID=MsG0480023827.01.T01;Parent=MsG0480023827.01;Name=MsG0480023827.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|0\|0\|1\|0.33\|0.25\|4\|0\|290
Chr4	exon	90217108	90217321	90217108	ID=MsG0480023827.01.T01:exon:4044;Parent=MsG0480023827.01.T01
Chr4	exon	90216589	90216657	90216589	ID=MsG0480023827.01.T01:exon:4043;Parent=MsG0480023827.01.T01
Chr4	exon	90216240	90216420	90216240	ID=MsG0480023827.01.T01:exon:4042;Parent=MsG0480023827.01.T01
Chr4	exon	90215706	90216114	90215706	ID=MsG0480023827.01.T01:exon:4041;Parent=MsG0480023827.01.T01
Chr4	CDS	90217108	90217321	90217108	ID=MsG0480023827.01.T01:cds;Parent=MsG0480023827.01.T01
Chr4	CDS	90216589	90216657	90216589	ID=MsG0480023827.01.T01:cds;Parent=MsG0480023827.01.T01
Chr4	CDS	90216240	90216420	90216240	ID=MsG0480023827.01.T01:cds;Parent=MsG0480023827.01.T01
Chr4	CDS	90215706	90216114	90215706	ID=MsG0480023827.01.T01:cds;Parent=MsG0480023827.01.T01

Gene Sequence

>MsG0480023827.01.T01

ATGATCAAATTTGAAAAAATGTTGGTAGCTTTTTTTATCTTTGCATTGATGATAACCAACATAATCCAGGTGAATGCCAACTTTACAAAAAGCATGTACCTCACCTGGGGTGCTCAACATGCATCAATTGTGGGTGAAGATCTTCACCTAGTCTTAGATAAAACCTCAGGTGAAAAAATTTATATTTACCACGCATCAGTTTTGGTTTCAATAGGATCAGCTGCTCGCTCAAAGAGATCTTTCTTATTTGGAAGTATTGAAATGTTAATAAAGCTGATACCTGGAAGCCAGCATGATGAGATAGATTTTGAGTTCTTAGGTAATAGTACAGGACAACCATACACAGTGAATACTAACCTATTTACACAAGGAAAAGGGAGCAGAGAGCAACAATTTCACCTCTGGTTTGACCCAACTGCTGATTTTCACAATTACACCATTCACTGGAATCCCACAGAAATTGTGTGGTATGTTGATAGCATACCAATACGGGTTTTCAGGAACTACGAACACGAAGGCATTGCTTATCCAAACAAGCAAGGAATGAGGGTTTATACAAGCCTATGGAATGCAGATAACTGGGCAACAAGAGGCGGGCTTGTGAAAACCAATTGGAGCAATGCACCATTCAAAGTAGGGTTTCATCATTTCAGAGCAAGGGCTTGTAAGTGGAATGGAGCAGCAAGCATTAACCAATGTGCCTCTAATGTCAAAGCAAATTGGTGGACATCTTCAGTATACAAACACCTTAGTTATGGCATGATAAGGCAGTTGAATTGGGTGCAGAAGAATTTCATGACCTATGACTATTGCAAAGATTACAAAAGATTCAACGGACACATCCCTCATGAATGCTTCAAAACACAATTCTAA

Protein sequence

>MsG0480023827.01.T01

MIKFEKMLVAFFIFALMITNIIQVNANFTKSMYLTWGAQHASIVGEDLHLVLDKTSGEKIYIYHASVLVSIGSAARSKRSFLFGSIEMLIKLIPGSQHDEIDFEFLGNSTGQPYTVNTNLFTQGKGSREQQFHLWFDPTADFHNYTIHWNPTEIVWYVDSIPIRVFRNYEHEGIAYPNKQGMRVYTSLWNADNWATRGGLVKTNWSNAPFKVGFHHFRARACKWNGAASINQCASNVKANWWTSSVYKHLSYGMIRQLNWVQKNFMTYDYCKDYKRFNGHIPHECFKTQF*