Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023827.01.T01 | XP_003630750.2 | 87.869 | 305 | 7 | 2 | 1 | 290 | 1 | 290 | 0 | 550 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023827.01.T01 | Q9SVV2 | 52.492 | 301 | 112 | 3 | 6 | 290 | 7 | 292 | 1.70E-111 | 327 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023827.01.T01 | G7L858 | 87.625 | 299 | 7 | 2 | 7 | 290 | 1 | 284 | 0.0 | 538 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880046024.01 | MsG0480023827.01 | PPI |
| MsG0880046025.01 | MsG0480023827.01 | PPI |
| MsG0880046024.01 | MsG0480023827.01 | PPI |
| MsG0480023827.01 | MsG0880042313.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023827.01.T01 | MTR_8g102910 | 87.625 | 299 | 7 | 2 | 7 | 290 | 1 | 284 | 0.0 | 538 |
| MsG0480023827.01.T01 | MTR_6g088320 | 68.197 | 305 | 65 | 4 | 1 | 290 | 1 | 288 | 2.68e-155 | 435 |
| MsG0480023827.01.T01 | MTR_2g038860 | 44.625 | 307 | 134 | 6 | 2 | 289 | 1 | 290 | 1.67e-87 | 263 |
| MsG0480023827.01.T01 | MTR_2g038705 | 44.625 | 307 | 134 | 6 | 2 | 289 | 1 | 290 | 1.67e-87 | 263 |
| MsG0480023827.01.T01 | MTR_4g128410 | 45.066 | 304 | 129 | 5 | 2 | 288 | 1 | 283 | 1.71e-86 | 260 |
| MsG0480023827.01.T01 | MTR_7g093530 | 46.048 | 291 | 121 | 4 | 12 | 285 | 8 | 279 | 4.67e-85 | 256 |
| MsG0480023827.01.T01 | MTR_4g128570 | 45.270 | 296 | 110 | 6 | 7 | 285 | 12 | 272 | 1.04e-83 | 253 |
| MsG0480023827.01.T01 | MTR_4g128580 | 46.853 | 286 | 114 | 6 | 20 | 285 | 18 | 285 | 4.75e-82 | 249 |
| MsG0480023827.01.T01 | MTR_4g128400 | 45.035 | 282 | 119 | 4 | 21 | 285 | 21 | 283 | 4.32e-81 | 246 |
| MsG0480023827.01.T01 | MTR_3g084630 | 44.118 | 306 | 126 | 7 | 2 | 290 | 5 | 282 | 5.58e-81 | 246 |
| MsG0480023827.01.T01 | MTR_3g084620 | 44.118 | 306 | 126 | 7 | 2 | 290 | 5 | 282 | 6.29e-81 | 246 |
| MsG0480023827.01.T01 | MTR_8g081040 | 45.238 | 294 | 119 | 7 | 10 | 285 | 12 | 281 | 1.55e-80 | 245 |
| MsG0480023827.01.T01 | MTR_4g126920 | 45.126 | 277 | 117 | 5 | 26 | 285 | 23 | 281 | 8.43e-80 | 243 |
| MsG0480023827.01.T01 | MTR_4g097640 | 42.761 | 297 | 130 | 8 | 10 | 286 | 12 | 288 | 2.85e-76 | 234 |
| MsG0480023827.01.T01 | MTR_4g097670 | 42.761 | 297 | 130 | 8 | 10 | 286 | 12 | 288 | 2.85e-76 | 234 |
| MsG0480023827.01.T01 | MTR_4g097700 | 44.444 | 279 | 117 | 7 | 26 | 286 | 67 | 325 | 1.30e-75 | 234 |
| MsG0480023827.01.T01 | MTR_4g128590 | 42.657 | 286 | 136 | 6 | 7 | 285 | 11 | 275 | 3.57e-75 | 231 |
| MsG0480023827.01.T01 | MTR_7g084760 | 40.397 | 302 | 140 | 7 | 7 | 289 | 11 | 291 | 5.69e-75 | 231 |
| MsG0480023827.01.T01 | MTR_7g084770 | 39.610 | 308 | 136 | 8 | 7 | 285 | 1 | 287 | 1.81e-73 | 227 |
| MsG0480023827.01.T01 | MTR_7g084750 | 40.000 | 300 | 144 | 6 | 7 | 289 | 11 | 291 | 6.30e-73 | 226 |
| MsG0480023827.01.T01 | MTR_6g033085 | 39.934 | 303 | 141 | 8 | 7 | 289 | 10 | 291 | 7.88e-72 | 223 |
| MsG0480023827.01.T01 | MTR_6g033055 | 39.274 | 303 | 143 | 8 | 7 | 289 | 10 | 291 | 2.28e-71 | 222 |
| MsG0480023827.01.T01 | MTR_1g105900 | 38.742 | 302 | 150 | 5 | 1 | 285 | 8 | 291 | 2.43e-70 | 219 |
| MsG0480023827.01.T01 | MTR_8g102220 | 40.850 | 306 | 122 | 7 | 2 | 289 | 6 | 270 | 7.04e-70 | 218 |
| MsG0480023827.01.T01 | MTR_4g045853 | 39.116 | 294 | 143 | 5 | 12 | 285 | 11 | 288 | 3.13e-67 | 211 |
| MsG0480023827.01.T01 | MTR_2g089140 | 38.000 | 300 | 145 | 6 | 8 | 287 | 10 | 288 | 3.56e-67 | 211 |
| MsG0480023827.01.T01 | MTR_3g089540 | 45.022 | 231 | 105 | 3 | 72 | 287 | 61 | 284 | 3.56e-66 | 209 |
| MsG0480023827.01.T01 | MTR_8g013055 | 40.892 | 269 | 136 | 7 | 28 | 286 | 31 | 286 | 4.48e-65 | 206 |
| MsG0480023827.01.T01 | MTR_2g089140 | 43.805 | 226 | 106 | 4 | 82 | 287 | 1 | 225 | 7.65e-65 | 203 |
| MsG0480023827.01.T01 | MTR_1g105495 | 41.322 | 242 | 121 | 3 | 62 | 285 | 56 | 294 | 5.96e-64 | 203 |
| MsG0480023827.01.T01 | MTR_5g091730 | 42.358 | 229 | 108 | 3 | 72 | 285 | 59 | 278 | 4.21e-63 | 201 |
| MsG0480023827.01.T01 | MTR_7g056090 | 34.007 | 297 | 151 | 9 | 7 | 285 | 12 | 281 | 9.61e-49 | 164 |
| MsG0480023827.01.T01 | MTR_4g057450 | 34.211 | 304 | 162 | 9 | 8 | 285 | 13 | 304 | 3.56e-48 | 162 |
| MsG0480023827.01.T01 | MTR_2g049130 | 33.457 | 269 | 165 | 8 | 8 | 271 | 9 | 268 | 1.57e-47 | 160 |
| MsG0480023827.01.T01 | MTR_2g095800 | 35.659 | 258 | 148 | 8 | 36 | 285 | 62 | 309 | 3.90e-47 | 160 |
| MsG0480023827.01.T01 | MTR_2g049030 | 35.766 | 274 | 153 | 11 | 9 | 271 | 6 | 267 | 3.02e-46 | 157 |
| MsG0480023827.01.T01 | MTR_7g102260 | 35.106 | 282 | 134 | 8 | 27 | 285 | 36 | 291 | 2.93e-43 | 149 |
| MsG0480023827.01.T01 | MTR_1g063020 | 35.740 | 277 | 121 | 9 | 36 | 285 | 48 | 294 | 3.03e-43 | 150 |
| MsG0480023827.01.T01 | MTR_1g033420 | 31.438 | 299 | 179 | 10 | 2 | 285 | 4 | 291 | 2.35e-39 | 140 |
| MsG0480023827.01.T01 | MTR_5g029100 | 37.306 | 193 | 112 | 7 | 98 | 285 | 86 | 274 | 7.54e-36 | 131 |
| MsG0480023827.01.T01 | MTR_8g090100 | 31.818 | 286 | 170 | 12 | 12 | 285 | 9 | 281 | 8.15e-35 | 128 |
| MsG0480023827.01.T01 | MTR_5g029100 | 37.824 | 193 | 111 | 7 | 98 | 285 | 117 | 305 | 1.92e-34 | 128 |
| MsG0480023827.01.T01 | MTR_8g064180 | 29.921 | 254 | 147 | 6 | 50 | 285 | 70 | 310 | 2.28e-34 | 127 |
| MsG0480023827.01.T01 | MTR_8g479340 | 42.857 | 98 | 53 | 2 | 188 | 285 | 41 | 135 | 1.11e-19 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023827.01.T01 | AT4G28850 | 52.492 | 301 | 112 | 3 | 6 | 290 | 7 | 292 | 1.73e-112 | 327 |
| MsG0480023827.01.T01 | AT5G57560 | 45.638 | 298 | 122 | 8 | 8 | 286 | 6 | 282 | 2.14e-85 | 258 |
| MsG0480023827.01.T01 | AT4G25810 | 44.737 | 304 | 128 | 6 | 1 | 286 | 3 | 284 | 5.05e-85 | 256 |
| MsG0480023827.01.T01 | AT5G57530 | 47.482 | 278 | 108 | 6 | 26 | 285 | 25 | 282 | 4.27e-83 | 252 |
| MsG0480023827.01.T01 | AT5G57550 | 43.813 | 299 | 121 | 6 | 12 | 285 | 6 | 282 | 8.15e-82 | 248 |
| MsG0480023827.01.T01 | AT3G23730 | 45.070 | 284 | 120 | 5 | 25 | 289 | 24 | 290 | 1.26e-81 | 248 |
| MsG0480023827.01.T01 | AT2G18800 | 41.720 | 314 | 136 | 7 | 1 | 285 | 1 | 296 | 6.42e-81 | 247 |
| MsG0480023827.01.T01 | AT4G14130 | 45.230 | 283 | 116 | 5 | 26 | 289 | 26 | 288 | 1.63e-79 | 243 |
| MsG0480023827.01.T01 | AT5G57540 | 45.126 | 277 | 116 | 5 | 26 | 285 | 24 | 281 | 2.32e-79 | 242 |
| MsG0480023827.01.T01 | AT4G25820 | 44.186 | 301 | 111 | 8 | 9 | 285 | 18 | 285 | 2.39e-79 | 242 |
| MsG0480023827.01.T01 | AT4G30270 | 43.151 | 292 | 116 | 5 | 11 | 285 | 7 | 265 | 6.40e-79 | 240 |
| MsG0480023827.01.T01 | AT4G37800 | 42.712 | 295 | 131 | 7 | 9 | 285 | 15 | 289 | 3.39e-75 | 232 |
| MsG0480023827.01.T01 | AT5G48070 | 40.789 | 304 | 138 | 7 | 1 | 285 | 1 | 281 | 6.67e-74 | 228 |
| MsG0480023827.01.T01 | AT2G14620 | 40.000 | 300 | 142 | 5 | 7 | 285 | 12 | 294 | 4.18e-73 | 227 |
| MsG0480023827.01.T01 | AT5G65730 | 41.554 | 296 | 135 | 6 | 8 | 285 | 15 | 290 | 5.87e-73 | 226 |
| MsG0480023827.01.T01 | AT5G13870 | 39.344 | 305 | 142 | 8 | 7 | 289 | 8 | 291 | 9.04e-73 | 226 |
| MsG0480023827.01.T01 | AT5G13870 | 39.344 | 305 | 142 | 8 | 7 | 289 | 8 | 291 | 9.04e-73 | 226 |
| MsG0480023827.01.T01 | AT5G13870 | 39.344 | 305 | 142 | 8 | 7 | 289 | 8 | 291 | 9.04e-73 | 226 |
| MsG0480023827.01.T01 | AT2G14620 | 45.726 | 234 | 110 | 2 | 67 | 285 | 13 | 244 | 1.05e-71 | 221 |
| MsG0480023827.01.T01 | AT4G30290 | 47.162 | 229 | 98 | 5 | 72 | 285 | 56 | 276 | 1.97e-69 | 216 |
| MsG0480023827.01.T01 | AT2G14620 | 46.606 | 221 | 101 | 2 | 80 | 285 | 15 | 233 | 1.08e-68 | 213 |
| MsG0480023827.01.T01 | AT4G30280 | 41.297 | 293 | 134 | 8 | 10 | 285 | 10 | 281 | 2.71e-68 | 214 |
| MsG0480023827.01.T01 | AT2G06850 | 39.000 | 300 | 142 | 7 | 8 | 285 | 10 | 290 | 1.11e-67 | 213 |
| MsG0480023827.01.T01 | AT4G03210 | 38.870 | 301 | 151 | 6 | 1 | 285 | 1 | 284 | 3.24e-67 | 211 |
| MsG0480023827.01.T01 | AT1G65310 | 46.522 | 230 | 98 | 6 | 72 | 285 | 61 | 281 | 6.40e-67 | 210 |
| MsG0480023827.01.T01 | AT1G65310 | 46.522 | 230 | 98 | 6 | 72 | 285 | 83 | 303 | 6.73e-67 | 211 |
| MsG0480023827.01.T01 | AT2G06850 | 44.017 | 234 | 112 | 4 | 67 | 285 | 1 | 230 | 4.38e-66 | 206 |
| MsG0480023827.01.T01 | AT4G03210 | 44.444 | 234 | 111 | 4 | 67 | 285 | 27 | 256 | 6.57e-66 | 207 |
| MsG0480023827.01.T01 | AT4G13090 | 38.591 | 298 | 150 | 7 | 4 | 285 | 8 | 288 | 1.99e-63 | 202 |
| MsG0480023827.01.T01 | AT4G13080 | 40.830 | 289 | 130 | 9 | 15 | 285 | 26 | 291 | 1.36e-62 | 199 |
| MsG0480023827.01.T01 | AT1G11545 | 36.093 | 302 | 153 | 7 | 8 | 285 | 14 | 299 | 1.86e-62 | 199 |
| MsG0480023827.01.T01 | AT3G25050 | 38.298 | 282 | 137 | 7 | 22 | 286 | 29 | 290 | 6.96e-60 | 192 |
| MsG0480023827.01.T01 | AT3G44990 | 34.276 | 283 | 137 | 8 | 26 | 285 | 37 | 293 | 2.06e-46 | 158 |
| MsG0480023827.01.T01 | AT2G36870 | 35.417 | 288 | 129 | 9 | 27 | 287 | 42 | 299 | 3.43e-45 | 155 |
| MsG0480023827.01.T01 | AT1G10550 | 32.210 | 267 | 156 | 7 | 36 | 285 | 51 | 309 | 9.27e-39 | 138 |
| MsG0480023827.01.T01 | AT1G32170 | 37.500 | 192 | 111 | 7 | 98 | 285 | 107 | 293 | 1.19e-36 | 134 |
| MsG0480023827.01.T01 | AT1G14720 | 29.348 | 276 | 170 | 8 | 14 | 277 | 21 | 283 | 1.82e-35 | 130 |
| MsG0480023827.01.T01 | AT2G01850 | 27.986 | 293 | 187 | 8 | 1 | 278 | 1 | 284 | 2.13e-35 | 130 |
| MsG0480023827.01.T01 | AT3G48580 | 32.296 | 257 | 148 | 11 | 33 | 285 | 39 | 273 | 4.15e-34 | 125 |
| MsG0480023827.01.T01 | AT4G18990 | 32.338 | 201 | 122 | 4 | 97 | 285 | 40 | 238 | 3.05e-32 | 120 |
| MsG0480023827.01.T01 | AT4G18990 | 32.338 | 201 | 122 | 4 | 97 | 285 | 114 | 312 | 1.68e-31 | 120 |
| MsG0480023827.01.T01 | AT3G48580 | 34.211 | 190 | 106 | 8 | 96 | 285 | 44 | 214 | 6.94e-31 | 115 |
| MsG0480023827.01.T01 | AT2G36870 | 36.842 | 152 | 58 | 3 | 27 | 155 | 42 | 178 | 8.82e-22 | 90.5 |
| MsG0480023827.01.T01 | AT3G48580 | 28.405 | 257 | 138 | 12 | 33 | 285 | 39 | 253 | 1.46e-19 | 86.3 |
Find 62 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAAGAGATCTTTCTTATT+TGG | 0.192788 | 4:-90216621 | MsG0480023827.01.T01:CDS |
| CCAATTTGCTTTGACATTAG+AGG | 0.263371 | 4:+90215856 | None:intergenic |
| GCCTATGGAATGCAGATAAC+TGG | 0.288054 | 4:-90215997 | MsG0480023827.01.T01:CDS |
| ACCCTCATTCCTTGCTTGTT+TGG | 0.311158 | 4:+90216028 | None:intergenic |
| TATTTACCACGCATCAGTTT+TGG | 0.337913 | 4:-90217117 | MsG0480023827.01.T01:CDS |
| GTAATCTTTGCAATAGTCAT+AGG | 0.398884 | 4:+90215757 | None:intergenic |
| GCATGATAAGGCAGTTGAAT+TGG | 0.399529 | 4:-90215799 | MsG0480023827.01.T01:CDS |
| AGGTTAGTATTCACTGTGTA+TGG | 0.414598 | 4:+90216345 | None:intergenic |
| CCTCTAATGTCAAAGCAAAT+TGG | 0.421458 | 4:-90215856 | MsG0480023827.01.T01:CDS |
| AACCTATTTACACAAGGAAA+AGG | 0.421723 | 4:-90216327 | MsG0480023827.01.T01:CDS |
| GAATGGTGCATTGCTCCAAT+TGG | 0.443865 | 4:+90215949 | None:intergenic |
| GTATACAAACACCTTAGTTA+TGG | 0.462494 | 4:-90215821 | MsG0480023827.01.T01:CDS |
| TTCACAATTACACCATTCAC+TGG | 0.466433 | 4:-90216257 | MsG0480023827.01.T01:CDS |
| GAGAGCAACAATTTCACCTC+TGG | 0.466634 | 4:-90216299 | MsG0480023827.01.T01:CDS |
| CCCAGTTATCTGCATTCCAT+AGG | 0.484000 | 4:+90215996 | None:intergenic |
| ACCTTAGTTATGGCATGATA+AGG | 0.484940 | 4:-90215811 | MsG0480023827.01.T01:CDS |
| TACAACTTACACAATTTCTG+TGG | 0.493964 | 4:+90216230 | None:intergenic |
| GCGGGCTTGTGAAAACCAAT+TGG | 0.494737 | 4:-90215964 | MsG0480023827.01.T01:CDS |
| TTCTGTGGGATTCCAGTGAA+TGG | 0.501704 | 4:+90216245 | None:intergenic |
| TAGTTCCTGAAAACCCGTAT+TGG | 0.504438 | 4:+90216073 | None:intergenic |
| ACCTATTTACACAAGGAAAA+GGG | 0.504561 | 4:-90216326 | MsG0480023827.01.T01:CDS |
| TGTTGATAGCATACCAATAC+GGG | 0.505198 | 4:-90216086 | MsG0480023827.01.T01:CDS |
| TCAGAGCAAGGGCTTGTAAG+TGG | 0.508970 | 4:-90215907 | MsG0480023827.01.T01:CDS |
| CATGATAAGGCAGTTGAATT+GGG | 0.509250 | 4:-90215798 | MsG0480023827.01.T01:CDS |
| ATGTTGATAGCATACCAATA+CGG | 0.513359 | 4:-90216087 | MsG0480023827.01.T01:CDS |
| GCCTTATCATGCCATAACTA+AGG | 0.515574 | 4:+90215810 | None:intergenic |
| TTGTAAAGTTGGCATTCACC+TGG | 0.515890 | 4:+90217234 | None:intergenic |
| TGAGGGTTTATACAAGCCTA+TGG | 0.519652 | 4:-90216012 | MsG0480023827.01.T01:CDS |
| GTTTCATCATTTCAGAGCAA+GGG | 0.521943 | 4:-90215918 | MsG0480023827.01.T01:CDS |
| TGCTCAACATGCATCAATTG+TGG | 0.523657 | 4:-90217189 | MsG0480023827.01.T01:CDS |
| TGATGAAACCCTACTTTGAA+TGG | 0.534214 | 4:+90215932 | None:intergenic |
| CTTTGACATTAGAGGCACAT+TGG | 0.538440 | 4:+90215864 | None:intergenic |
| GGTTTCATCATTTCAGAGCA+AGG | 0.539340 | 4:-90215919 | MsG0480023827.01.T01:CDS |
| GAGTTCTTAGGTAATAGTAC+AGG | 0.539738 | 4:-90216372 | MsG0480023827.01.T01:CDS |
| GGCATTCACCTGGATTATGT+TGG | 0.541012 | 4:+90217244 | None:intergenic |
| GCAATGCACCATTCAAAGTA+GGG | 0.541020 | 4:-90215940 | MsG0480023827.01.T01:CDS |
| ACAACTTACACAATTTCTGT+GGG | 0.541852 | 4:+90216231 | None:intergenic |
| AGCAATGCACCATTCAAAGT+AGG | 0.545062 | 4:-90215941 | MsG0480023827.01.T01:CDS |
| CTAATGTCAAAGCAAATTGG+TGG | 0.545921 | 4:-90215853 | MsG0480023827.01.T01:CDS |
| TCCAAACAAGCAAGGAATGA+GGG | 0.547149 | 4:-90216029 | MsG0480023827.01.T01:CDS |
| GATGATAACCAACATAATCC+AGG | 0.549208 | 4:-90217252 | MsG0480023827.01.T01:CDS |
| TGATGCATGTTGAGCACCCC+AGG | 0.551286 | 4:+90217196 | None:intergenic |
| TAATTGTGAAAATCAGCAGT+TGG | 0.553093 | 4:+90216270 | None:intergenic |
| AATTGTGAAAATCAGCAGTT+GGG | 0.553882 | 4:+90216271 | None:intergenic |
| CAAAATCTATCTCATCATGC+TGG | 0.555369 | 4:+90216394 | None:intergenic |
| ATCCAAACAAGCAAGGAATG+AGG | 0.556059 | 4:-90216030 | MsG0480023827.01.T01:CDS |
| ATAACTGGGCAACAAGAGGC+GGG | 0.564075 | 4:-90215982 | MsG0480023827.01.T01:CDS |
| GCAAGGGCTTGTAAGTGGAA+TGG | 0.565324 | 4:-90215902 | MsG0480023827.01.T01:CDS |
| CCTATGGAATGCAGATAACT+GGG | 0.569064 | 4:-90215996 | MsG0480023827.01.T01:CDS |
| AAAGATTACAAAAGATTCAA+CGG | 0.569128 | 4:-90215743 | MsG0480023827.01.T01:CDS |
| CTAGTCTTAGATAAAACCTC+AGG | 0.572459 | 4:-90217152 | MsG0480023827.01.T01:CDS |
| GCTCAACATGCATCAATTGT+GGG | 0.576736 | 4:-90217188 | MsG0480023827.01.T01:CDS |
| GATAACTGGGCAACAAGAGG+CGG | 0.582475 | 4:-90215983 | MsG0480023827.01.T01:CDS |
| ATGTTAATAAAGCTGATACC+TGG | 0.590614 | 4:-90216588 | MsG0480023827.01.T01:intron |
| CATGTTGAGCACCCCAGGTG+AGG | 0.613876 | 4:+90217201 | None:intergenic |
| GCAGATAACTGGGCAACAAG+AGG | 0.623642 | 4:-90215986 | MsG0480023827.01.T01:CDS |
| CAGCAGTTGGGTCAAACCAG+AGG | 0.644797 | 4:+90216283 | None:intergenic |
| ATTGCTTATCCAAACAAGCA+AGG | 0.650045 | 4:-90216037 | MsG0480023827.01.T01:CDS |
| TTGAAACCAAAACTGATGCG+TGG | 0.664040 | 4:+90217111 | None:intergenic |
| TTCAGGAACTACGAACACGA+AGG | 0.708811 | 4:-90216061 | MsG0480023827.01.T01:CDS |
| AATACTAACCTATTTACACA+AGG | 0.711992 | 4:-90216333 | MsG0480023827.01.T01:CDS |
| AAAAGCATGTACCTCACCTG+GGG | 0.718109 | 4:-90217212 | MsG0480023827.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTAAATTTTGTCTCTTTT+TGG | + | Chr4:90216534-90216553 | None:intergenic | 15.0% |
| !!! | TTTAAATTTTGTCTCTTTTT+GGG | + | Chr4:90216533-90216552 | None:intergenic | 15.0% |
| !! | AAAGATTACAAAAGATTCAA+CGG | - | Chr4:90217262-90217281 | MsG0480023827.01.T01:CDS | 20.0% |
| !! | AGAAAAAAGAACAATAGAAA+AGG | + | Chr4:90216169-90216188 | None:intergenic | 20.0% |
| !! | GATCAAATTTGAAAAAATGT+TGG | - | Chr4:90215708-90215727 | MsG0480023827.01.T01:CDS | 20.0% |
| !! | GTGATTTATAAAAGAGATTA+TGG | + | Chr4:90216509-90216528 | None:intergenic | 20.0% |
| !!! | AAACATATTCATTGGTTTAT+TGG | - | Chr4:90216305-90216324 | MsG0480023827.01.T01:CDS | 20.0% |
| !!! | AATATAAATTTTTTCACCTG+AGG | + | Chr4:90215872-90215891 | None:intergenic | 20.0% |
| !!! | TTAAATTTTGTCTCTTTTTG+GGG | + | Chr4:90216532-90216551 | None:intergenic | 20.0% |
| ! | AATACTAACCTATTTACACA+AGG | - | Chr4:90216672-90216691 | MsG0480023827.01.T01:intron | 25.0% |
| ! | ATATAGATCAAGCAATTAGA+TGG | + | Chr4:90216870-90216889 | None:intergenic | 25.0% |
| ! | ATCATCATGACTATTATTTC+AGG | - | Chr4:90216891-90216910 | MsG0480023827.01.T01:intron | 25.0% |
| ! | TACAATGGAGAAAAAAGAAA+AGG | + | Chr4:90216202-90216221 | None:intergenic | 25.0% |
| ! | TCAAAGAGATCTTTCTTATT+TGG | - | Chr4:90216384-90216403 | MsG0480023827.01.T01:CDS | 25.0% |
| !! | TAAATTTTGTCTCTTTTTGG+GGG | + | Chr4:90216531-90216550 | None:intergenic | 25.0% |
| !! | TTTTCTTGCTAAAGAAAAGA+AGG | - | Chr4:90216077-90216096 | MsG0480023827.01.T01:CDS | 25.0% |
| !!! | TGAACATTTTTTTGGTACAA+TGG | + | Chr4:90216217-90216236 | None:intergenic | 25.0% |
| AACCTATTTACACAAGGAAA+AGG | - | Chr4:90216678-90216697 | MsG0480023827.01.T01:intron | 30.0% | |
| AAGCAATTAGATGGTAACTT+GGG | + | Chr4:90216861-90216880 | None:intergenic | 30.0% | |
| AATTGTGAAAATCAGCAGTT+GGG | + | Chr4:90216737-90216756 | None:intergenic | 30.0% | |
| ACAACTTACACAATTTCTGT+GGG | + | Chr4:90216777-90216796 | None:intergenic | 30.0% | |
| ACCTATTTACACAAGGAAAA+GGG | - | Chr4:90216679-90216698 | MsG0480023827.01.T01:intron | 30.0% | |
| ACTGAGAGAAACATATTCAT+TGG | - | Chr4:90216297-90216316 | MsG0480023827.01.T01:CDS | 30.0% | |
| ATCAAGTGAACTTACATAGT+AGG | + | Chr4:90216466-90216485 | None:intergenic | 30.0% | |
| ATCATGACTATTATTTCAGG+TGG | - | Chr4:90216894-90216913 | MsG0480023827.01.T01:intron | 30.0% | |
| ATGTTAATAAAGCTGATACC+TGG | - | Chr4:90216417-90216436 | MsG0480023827.01.T01:intron | 30.0% | |
| GCTAATCTGGAAATATTACA+TGG | - | Chr4:90216327-90216346 | MsG0480023827.01.T01:CDS | 30.0% | |
| GTAATCTTTGCAATAGTCAT+AGG | + | Chr4:90217251-90217270 | None:intergenic | 30.0% | |
| GTATACAAACACCTTAGTTA+TGG | - | Chr4:90217184-90217203 | MsG0480023827.01.T01:CDS | 30.0% | |
| GTTGCAAATGAACATTTAGT+GGG | - | Chr4:90215983-90216002 | MsG0480023827.01.T01:CDS | 30.0% | |
| TAATTGTGAAAATCAGCAGT+TGG | + | Chr4:90216738-90216757 | None:intergenic | 30.0% | |
| TACAACTTACACAATTTCTG+TGG | + | Chr4:90216778-90216797 | None:intergenic | 30.0% | |
| TATGTTAGATCTTACTGTTC+TGG | + | Chr4:90216562-90216581 | None:intergenic | 30.0% | |
| TGTTGCAAATGAACATTTAG+TGG | - | Chr4:90215982-90216001 | MsG0480023827.01.T01:CDS | 30.0% | |
| TTGCAAATGAACATTTAGTG+GGG | - | Chr4:90215984-90216003 | MsG0480023827.01.T01:CDS | 30.0% | |
| ! | AAGAAAAGAAGGTTTTCTCA+TGG | - | Chr4:90216088-90216107 | MsG0480023827.01.T01:CDS | 30.0% |
| !! | ATGTTGATAGCATACCAATA+CGG | - | Chr4:90216918-90216937 | MsG0480023827.01.T01:intron | 30.0% |
| !! | GTACATGCTTTTTGTAAAGT+TGG | + | Chr4:90215785-90215804 | None:intergenic | 30.0% |
| !!! | GAGATAGATTTTGAGTTCTT+AGG | - | Chr4:90216621-90216640 | MsG0480023827.01.T01:CDS | 30.0% |
| !!! | GCGATACATGAACATTTTTT+TGG | + | Chr4:90216225-90216244 | None:intergenic | 30.0% |
| AATTCACCTTCTCAGTTACA+TGG | - | Chr4:90216811-90216830 | MsG0480023827.01.T01:intron | 35.0% | |
| ACCTTAGTTATGGCATGATA+AGG | - | Chr4:90217194-90217213 | MsG0480023827.01.T01:CDS | 35.0% | |
| AGGTTAGTATTCACTGTGTA+TGG | + | Chr4:90216663-90216682 | None:intergenic | 35.0% | |
| ATTGCTTATCCAAACAAGCA+AGG | - | Chr4:90216968-90216987 | MsG0480023827.01.T01:intron | 35.0% | |
| ATTTGCCACATTCTTCATGA+AGG | - | Chr4:90216039-90216058 | MsG0480023827.01.T01:CDS | 35.0% | |
| CAAAATCTATCTCATCATGC+TGG | + | Chr4:90216614-90216633 | None:intergenic | 35.0% | |
| CAAGCAATTAGATGGTAACT+TGG | + | Chr4:90216862-90216881 | None:intergenic | 35.0% | |
| CATGATAAGGCAGTTGAATT+GGG | - | Chr4:90217207-90217226 | MsG0480023827.01.T01:CDS | 35.0% | |
| CCTCTAATGTCAAAGCAAAT+TGG | - | Chr4:90217149-90217168 | MsG0480023827.01.T01:CDS | 35.0% | |
| CTAATGTCAAAGCAAATTGG+TGG | - | Chr4:90217152-90217171 | MsG0480023827.01.T01:CDS | 35.0% | |
| GAGTTCTTAGGTAATAGTAC+AGG | - | Chr4:90216633-90216652 | MsG0480023827.01.T01:CDS | 35.0% | |
| GATGATAACCAACATAATCC+AGG | - | Chr4:90215753-90215772 | MsG0480023827.01.T01:CDS | 35.0% | |
| GTTTCATCATTTCAGAGCAA+GGG | - | Chr4:90217087-90217106 | MsG0480023827.01.T01:intron | 35.0% | |
| TGATGAAACCCTACTTTGAA+TGG | + | Chr4:90217076-90217095 | None:intergenic | 35.0% | |
| TGCAAATGAACATTTAGTGG+GGG | - | Chr4:90215985-90216004 | MsG0480023827.01.T01:CDS | 35.0% | |
| TTCACAATTACACCATTCAC+TGG | - | Chr4:90216748-90216767 | MsG0480023827.01.T01:intron | 35.0% | |
| TTTGCCACATTCTTCATGAA+GGG | - | Chr4:90216040-90216059 | MsG0480023827.01.T01:CDS | 35.0% | |
| ! | CCAATTTGCTTTGACATTAG+AGG | + | Chr4:90217152-90217171 | None:intergenic | 35.0% |
| ! | GTTTTCTCATGGCTTTCTTA+AGG | - | Chr4:90216099-90216118 | MsG0480023827.01.T01:intron | 35.0% |
| ! | TATTTACCACGCATCAGTTT+TGG | - | Chr4:90215888-90215907 | MsG0480023827.01.T01:CDS | 35.0% |
| ! | TCCCTTTTCCTTGTGTAAAT+AGG | + | Chr4:90216683-90216702 | None:intergenic | 35.0% |
| !! | CATTGGTTTATTGGCTAATC+TGG | - | Chr4:90216314-90216333 | MsG0480023827.01.T01:CDS | 35.0% |
| !! | CTAGTCTTAGATAAAACCTC+AGG | - | Chr4:90215853-90215872 | MsG0480023827.01.T01:CDS | 35.0% |
| !! | CTGAGGTTTTATCTAAGACT+AGG | + | Chr4:90215855-90215874 | None:intergenic | 35.0% |
| !! | TGTTGATAGCATACCAATAC+GGG | - | Chr4:90216919-90216938 | MsG0480023827.01.T01:intron | 35.0% |
| !!! | GCATCAGTTTTGGTTTCAAT+AGG | - | Chr4:90215898-90215917 | MsG0480023827.01.T01:CDS | 35.0% |
| !!! | GTGTTTTGAAGCATTCATGA+GGG | + | Chr4:90217294-90217313 | None:intergenic | 35.0% |
| !!! | TGTGTTTTGAAGCATTCATG+AGG | + | Chr4:90217295-90217314 | None:intergenic | 35.0% |
| AGCAATGCACCATTCAAAGT+AGG | - | Chr4:90217064-90217083 | MsG0480023827.01.T01:intron | 40.0% | |
| AGTTCCCTTCATGAAGAATG+TGG | + | Chr4:90216047-90216066 | None:intergenic | 40.0% | |
| ATCCAAACAAGCAAGGAATG+AGG | - | Chr4:90216975-90216994 | MsG0480023827.01.T01:intron | 40.0% | |
| CAGCTGATCCTGAAATTCAA+AGG | + | Chr4:90216359-90216378 | None:intergenic | 40.0% | |
| CCTATGGAATGCAGATAACT+GGG | - | Chr4:90217009-90217028 | MsG0480023827.01.T01:intron | 40.0% | |
| GAAGAATGTGGCAAATTCTC+TGG | + | Chr4:90216035-90216054 | None:intergenic | 40.0% | |
| GCAAATGAACATTTAGTGGG+GGG | - | Chr4:90215986-90216005 | MsG0480023827.01.T01:CDS | 40.0% | |
| GCAATGCACCATTCAAAGTA+GGG | - | Chr4:90217065-90217084 | MsG0480023827.01.T01:intron | 40.0% | |
| GCATGATAAGGCAGTTGAAT+TGG | - | Chr4:90217206-90217225 | MsG0480023827.01.T01:CDS | 40.0% | |
| GCCTTATCATGCCATAACTA+AGG | + | Chr4:90217198-90217217 | None:intergenic | 40.0% | |
| GCTCAACATGCATCAATTGT+GGG | - | Chr4:90215817-90215836 | MsG0480023827.01.T01:CDS | 40.0% | |
| GGTTGTTGCCTTTGAATTTC+AGG | - | Chr4:90216348-90216367 | MsG0480023827.01.T01:CDS | 40.0% | |
| GGTTTCATCATTTCAGAGCA+AGG | - | Chr4:90217086-90217105 | MsG0480023827.01.T01:intron | 40.0% | |
| GTGGCAAATTCTCTGGTATT+AGG | + | Chr4:90216028-90216047 | None:intergenic | 40.0% | |
| TAGTTCCTGAAAACCCGTAT+TGG | + | Chr4:90216935-90216954 | None:intergenic | 40.0% | |
| TCCAAACAAGCAAGGAATGA+GGG | - | Chr4:90216976-90216995 | MsG0480023827.01.T01:intron | 40.0% | |
| TCTTTGCCATGTAACTGAGA+AGG | + | Chr4:90216820-90216839 | None:intergenic | 40.0% | |
| TGAGGGTTTATACAAGCCTA+TGG | - | Chr4:90216993-90217012 | MsG0480023827.01.T01:intron | 40.0% | |
| TGCTCAACATGCATCAATTG+TGG | - | Chr4:90215816-90215835 | MsG0480023827.01.T01:CDS | 40.0% | |
| TTCTTAAGGCTGATGCTATG+AGG | - | Chr4:90216113-90216132 | MsG0480023827.01.T01:intron | 40.0% | |
| TTGAAACCAAAACTGATGCG+TGG | + | Chr4:90215897-90215916 | None:intergenic | 40.0% | |
| TTGTAAAGTTGGCATTCACC+TGG | + | Chr4:90215774-90215793 | None:intergenic | 40.0% | |
| ! | AAAAAGCATGTACCTCACCT+GGG | - | Chr4:90215792-90215811 | MsG0480023827.01.T01:CDS | 40.0% |
| ! | CTTTGACATTAGAGGCACAT+TGG | + | Chr4:90217144-90217163 | None:intergenic | 40.0% |
| ACCCTCATTCCTTGCTTGTT+TGG | + | Chr4:90216980-90216999 | None:intergenic | 45.0% | |
| ACTATTCCTGCGGAATTACC+AGG | + | Chr4:90216438-90216457 | None:intergenic | 45.0% | |
| CCCAGTTATCTGCATTCCAT+AGG | + | Chr4:90217012-90217031 | None:intergenic | 45.0% | |
| GAAAGAAAGCCTAAGCACGT+GGG | + | Chr4:90216147-90216166 | None:intergenic | 45.0% | |
| GAATGGTGCATTGCTCCAAT+TGG | + | Chr4:90217059-90217078 | None:intergenic | 45.0% | |
| GAGAGCAACAATTTCACCTC+TGG | - | Chr4:90216706-90216725 | MsG0480023827.01.T01:intron | 45.0% | |
| GCCTATGGAATGCAGATAAC+TGG | - | Chr4:90217008-90217027 | MsG0480023827.01.T01:intron | 45.0% | |
| GGCATTCACCTGGATTATGT+TGG | + | Chr4:90215764-90215783 | None:intergenic | 45.0% | |
| TCCTGTCGAAGATAACTGTG+CGG | + | Chr4:90216589-90216608 | None:intergenic | 45.0% | |
| TTCAGGAACTACGAACACGA+AGG | - | Chr4:90216944-90216963 | MsG0480023827.01.T01:intron | 45.0% | |
| TTCTGTGGGATTCCAGTGAA+TGG | + | Chr4:90216763-90216782 | None:intergenic | 45.0% | |
| ! | AAAAGCATGTACCTCACCTG+GGG | - | Chr4:90215793-90215812 | MsG0480023827.01.T01:CDS | 45.0% |
| ! | CAAAAAGCATGTACCTCACC+TGG | - | Chr4:90215791-90215810 | MsG0480023827.01.T01:CDS | 45.0% |
| ! | GCATACCAATACGGGTTTTC+AGG | - | Chr4:90216927-90216946 | MsG0480023827.01.T01:intron | 45.0% |
| AAGCCTAAGCACGTGGGAAA+AGG | + | Chr4:90216141-90216160 | None:intergenic | 50.0% | |
| ATAACTGGGCAACAAGAGGC+GGG | - | Chr4:90217023-90217042 | MsG0480023827.01.T01:intron | 50.0% | |
| CTGATACCTGGTAATTCCGC+AGG | - | Chr4:90216429-90216448 | MsG0480023827.01.T01:intron | 50.0% | |
| GATAACTGGGCAACAAGAGG+CGG | - | Chr4:90217022-90217041 | MsG0480023827.01.T01:intron | 50.0% | |
| GCAAGGGCTTGTAAGTGGAA+TGG | - | Chr4:90217103-90217122 | MsG0480023827.01.T01:intron | 50.0% | |
| GCAGATAACTGGGCAACAAG+AGG | - | Chr4:90217019-90217038 | MsG0480023827.01.T01:intron | 50.0% | |
| GCGGGCTTGTGAAAACCAAT+TGG | - | Chr4:90217041-90217060 | MsG0480023827.01.T01:intron | 50.0% | |
| GGAAAGAAAGCCTAAGCACG+TGG | + | Chr4:90216148-90216167 | None:intergenic | 50.0% | |
| GTAGGCTGTGACTATTCCTG+CGG | + | Chr4:90216448-90216467 | None:intergenic | 50.0% | |
| TCAGAGCAAGGGCTTGTAAG+TGG | - | Chr4:90217098-90217117 | MsG0480023827.01.T01:intron | 50.0% | |
| TCCGCACAGTTATCTTCGAC+AGG | - | Chr4:90216585-90216604 | MsG0480023827.01.T01:intron | 50.0% | |
| ! | GATCCTTTTCCCACGTGCTT+AGG | - | Chr4:90216135-90216154 | MsG0480023827.01.T01:intron | 50.0% |
| CAGCAGTTGGGTCAAACCAG+AGG | + | Chr4:90216725-90216744 | None:intergenic | 55.0% | |
| TGATGCATGTTGAGCACCCC+AGG | + | Chr4:90215812-90215831 | None:intergenic | 55.0% | |
| CATGTTGAGCACCCCAGGTG+AGG | + | Chr4:90215807-90215826 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 90215706 | 90217321 | 90215706 | ID=MsG0480023827.01;Name=MsG0480023827.01 |
| Chr4 | mRNA | 90215706 | 90217321 | 90215706 | ID=MsG0480023827.01.T01;Parent=MsG0480023827.01;Name=MsG0480023827.01.T01;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|0.33|0.25|4|0|290 |
| Chr4 | exon | 90217108 | 90217321 | 90217108 | ID=MsG0480023827.01.T01:exon:4044;Parent=MsG0480023827.01.T01 |
| Chr4 | exon | 90216589 | 90216657 | 90216589 | ID=MsG0480023827.01.T01:exon:4043;Parent=MsG0480023827.01.T01 |
| Chr4 | exon | 90216240 | 90216420 | 90216240 | ID=MsG0480023827.01.T01:exon:4042;Parent=MsG0480023827.01.T01 |
| Chr4 | exon | 90215706 | 90216114 | 90215706 | ID=MsG0480023827.01.T01:exon:4041;Parent=MsG0480023827.01.T01 |
| Chr4 | CDS | 90217108 | 90217321 | 90217108 | ID=MsG0480023827.01.T01:cds;Parent=MsG0480023827.01.T01 |
| Chr4 | CDS | 90216589 | 90216657 | 90216589 | ID=MsG0480023827.01.T01:cds;Parent=MsG0480023827.01.T01 |
| Chr4 | CDS | 90216240 | 90216420 | 90216240 | ID=MsG0480023827.01.T01:cds;Parent=MsG0480023827.01.T01 |
| Chr4 | CDS | 90215706 | 90216114 | 90215706 | ID=MsG0480023827.01.T01:cds;Parent=MsG0480023827.01.T01 |
| Gene Sequence |
| Protein sequence |
