Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042313.01.T01 | RHN39079.1 | 93.562 | 233 | 15 | 0 | 1 | 233 | 1 | 233 | 2.86E-164 | 463 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042313.01.T01 | Q9SV60 | 65.487 | 226 | 76 | 1 | 8 | 231 | 63 | 288 | 5.61E-107 | 313 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042313.01.T01 | A0A396GBX7 | 93.562 | 233 | 15 | 0 | 1 | 233 | 1 | 233 | 1.37e-164 | 463 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880046024.01 | MsG0880042313.01 | PPI |
| MsG0880046025.01 | MsG0880042313.01 | PPI |
| MsG0880042313.01 | MsG0280010632.01 | PPI |
| MsG0880046024.01 | MsG0880042313.01 | PPI |
| MsG0480023827.01 | MsG0880042313.01 | PPI |
| MsG0880042313.01 | MsG0880045055.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042313.01.T01 | MTR_8g013055 | 93.805 | 226 | 14 | 0 | 8 | 233 | 62 | 287 | 1.05e-163 | 454 |
| MsG0880042313.01.T01 | MTR_7g056090 | 57.674 | 215 | 87 | 3 | 8 | 221 | 63 | 274 | 6.84e-87 | 259 |
| MsG0880042313.01.T01 | MTR_7g084760 | 52.655 | 226 | 104 | 3 | 8 | 231 | 63 | 287 | 2.36e-80 | 243 |
| MsG0880042313.01.T01 | MTR_4g097640 | 51.515 | 231 | 107 | 4 | 5 | 232 | 60 | 288 | 5.58e-80 | 241 |
| MsG0880042313.01.T01 | MTR_4g097670 | 51.515 | 231 | 107 | 4 | 5 | 232 | 60 | 288 | 5.58e-80 | 241 |
| MsG0880042313.01.T01 | MTR_4g097700 | 51.515 | 231 | 107 | 4 | 5 | 232 | 97 | 325 | 9.01e-80 | 242 |
| MsG0880042313.01.T01 | MTR_7g084770 | 52.212 | 226 | 105 | 3 | 8 | 231 | 63 | 287 | 1.62e-79 | 241 |
| MsG0880042313.01.T01 | MTR_6g033055 | 50.877 | 228 | 111 | 1 | 5 | 231 | 60 | 287 | 2.95e-79 | 240 |
| MsG0880042313.01.T01 | MTR_6g033085 | 50.877 | 228 | 111 | 1 | 5 | 231 | 60 | 287 | 4.22e-79 | 239 |
| MsG0880042313.01.T01 | MTR_7g084750 | 52.212 | 226 | 105 | 3 | 8 | 231 | 63 | 287 | 8.65e-79 | 239 |
| MsG0880042313.01.T01 | MTR_2g049030 | 47.748 | 222 | 110 | 4 | 5 | 225 | 58 | 274 | 8.45e-76 | 231 |
| MsG0880042313.01.T01 | MTR_4g045853 | 46.383 | 235 | 117 | 4 | 8 | 233 | 56 | 290 | 1.34e-75 | 231 |
| MsG0880042313.01.T01 | MTR_2g089140 | 45.726 | 234 | 118 | 3 | 8 | 232 | 54 | 287 | 3.12e-74 | 227 |
| MsG0880042313.01.T01 | MTR_1g105495 | 48.707 | 232 | 112 | 4 | 6 | 231 | 64 | 294 | 2.72e-72 | 222 |
| MsG0880042313.01.T01 | MTR_8g102910 | 49.351 | 231 | 110 | 5 | 8 | 232 | 51 | 280 | 6.23e-72 | 221 |
| MsG0880042313.01.T01 | MTR_2g049130 | 46.930 | 228 | 113 | 4 | 5 | 231 | 57 | 277 | 1.12e-71 | 220 |
| MsG0880042313.01.T01 | MTR_6g088320 | 49.784 | 231 | 109 | 5 | 8 | 232 | 55 | 284 | 4.14e-71 | 219 |
| MsG0880042313.01.T01 | MTR_2g089140 | 45.495 | 222 | 112 | 3 | 20 | 232 | 3 | 224 | 6.76e-71 | 216 |
| MsG0880042313.01.T01 | MTR_4g128570 | 48.673 | 226 | 99 | 4 | 8 | 231 | 62 | 272 | 2.48e-70 | 216 |
| MsG0880042313.01.T01 | MTR_4g128410 | 48.472 | 229 | 109 | 4 | 8 | 231 | 56 | 280 | 2.07e-69 | 214 |
| MsG0880042313.01.T01 | MTR_4g128400 | 49.565 | 230 | 107 | 4 | 8 | 232 | 59 | 284 | 2.68e-68 | 212 |
| MsG0880042313.01.T01 | MTR_4g128580 | 48.085 | 235 | 109 | 4 | 8 | 233 | 57 | 287 | 1.27e-67 | 210 |
| MsG0880042313.01.T01 | MTR_4g126920 | 47.162 | 229 | 113 | 4 | 8 | 231 | 56 | 281 | 1.70e-67 | 209 |
| MsG0880042313.01.T01 | MTR_1g105900 | 46.753 | 231 | 118 | 4 | 5 | 231 | 62 | 291 | 2.11e-67 | 210 |
| MsG0880042313.01.T01 | MTR_8g081040 | 46.753 | 231 | 109 | 4 | 8 | 233 | 62 | 283 | 2.19e-67 | 209 |
| MsG0880042313.01.T01 | MTR_7g093530 | 46.957 | 230 | 113 | 4 | 8 | 232 | 55 | 280 | 3.54e-67 | 209 |
| MsG0880042313.01.T01 | MTR_3g084620 | 47.598 | 229 | 105 | 4 | 8 | 231 | 59 | 277 | 1.65e-66 | 207 |
| MsG0880042313.01.T01 | MTR_3g084630 | 47.598 | 229 | 105 | 4 | 8 | 231 | 59 | 277 | 2.10e-66 | 207 |
| MsG0880042313.01.T01 | MTR_2g038860 | 45.957 | 235 | 113 | 4 | 8 | 232 | 57 | 287 | 7.22e-66 | 206 |
| MsG0880042313.01.T01 | MTR_2g038705 | 45.957 | 235 | 113 | 4 | 8 | 232 | 57 | 287 | 7.22e-66 | 206 |
| MsG0880042313.01.T01 | MTR_3g089540 | 47.788 | 226 | 112 | 3 | 8 | 231 | 61 | 282 | 2.65e-65 | 204 |
| MsG0880042313.01.T01 | MTR_4g128590 | 43.621 | 243 | 115 | 6 | 8 | 233 | 40 | 277 | 1.48e-63 | 199 |
| MsG0880042313.01.T01 | MTR_5g091730 | 47.137 | 227 | 110 | 5 | 8 | 231 | 59 | 278 | 3.50e-63 | 199 |
| MsG0880042313.01.T01 | MTR_8g102220 | 45.575 | 226 | 102 | 5 | 8 | 231 | 60 | 266 | 1.47e-61 | 194 |
| MsG0880042313.01.T01 | MTR_5g029100 | 40.598 | 234 | 128 | 5 | 8 | 231 | 42 | 274 | 1.66e-58 | 187 |
| MsG0880042313.01.T01 | MTR_5g029100 | 41.026 | 234 | 127 | 5 | 8 | 231 | 73 | 305 | 3.14e-58 | 188 |
| MsG0880042313.01.T01 | MTR_8g090100 | 38.889 | 234 | 131 | 4 | 8 | 232 | 52 | 282 | 6.80e-57 | 184 |
| MsG0880042313.01.T01 | MTR_1g033420 | 41.026 | 234 | 130 | 4 | 5 | 232 | 61 | 292 | 9.00e-57 | 184 |
| MsG0880042313.01.T01 | MTR_4g057450 | 42.035 | 226 | 117 | 5 | 6 | 223 | 77 | 296 | 4.07e-54 | 176 |
| MsG0880042313.01.T01 | MTR_2g095800 | 42.979 | 235 | 119 | 5 | 6 | 231 | 81 | 309 | 1.12e-52 | 172 |
| MsG0880042313.01.T01 | MTR_8g064180 | 38.298 | 235 | 133 | 5 | 8 | 231 | 77 | 310 | 4.31e-49 | 163 |
| MsG0880042313.01.T01 | MTR_7g102260 | 40.179 | 224 | 120 | 4 | 8 | 223 | 66 | 283 | 3.31e-48 | 160 |
| MsG0880042313.01.T01 | MTR_1g063020 | 38.793 | 232 | 118 | 5 | 5 | 223 | 66 | 286 | 3.53e-47 | 158 |
| MsG0880042313.01.T01 | MTR_8g479340 | 31.383 | 188 | 67 | 4 | 46 | 231 | 8 | 135 | 9.39e-21 | 85.1 |
| MsG0880042313.01.T01 | MTR_8g032640 | 34.559 | 136 | 82 | 4 | 8 | 141 | 42 | 172 | 3.45e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042313.01.T01 | AT4G13090 | 65.487 | 226 | 76 | 1 | 8 | 231 | 63 | 288 | 5.72e-108 | 313 |
| MsG0880042313.01.T01 | AT4G13080 | 62.054 | 224 | 83 | 2 | 8 | 231 | 70 | 291 | 2.96e-103 | 301 |
| MsG0880042313.01.T01 | AT3G25050 | 58.515 | 229 | 91 | 2 | 5 | 231 | 63 | 289 | 7.00e-98 | 287 |
| MsG0880042313.01.T01 | AT5G13870 | 52.212 | 226 | 105 | 3 | 8 | 231 | 63 | 287 | 2.52e-82 | 248 |
| MsG0880042313.01.T01 | AT5G13870 | 52.212 | 226 | 105 | 3 | 8 | 231 | 63 | 287 | 2.52e-82 | 248 |
| MsG0880042313.01.T01 | AT5G13870 | 52.212 | 226 | 105 | 3 | 8 | 231 | 63 | 287 | 2.52e-82 | 248 |
| MsG0880042313.01.T01 | AT2G06850 | 51.770 | 226 | 106 | 3 | 8 | 231 | 6 | 230 | 1.28e-81 | 244 |
| MsG0880042313.01.T01 | AT2G06850 | 51.770 | 226 | 106 | 3 | 8 | 231 | 66 | 290 | 4.12e-81 | 244 |
| MsG0880042313.01.T01 | AT4G37800 | 53.982 | 226 | 101 | 3 | 8 | 231 | 65 | 289 | 1.04e-80 | 244 |
| MsG0880042313.01.T01 | AT5G65730 | 50.435 | 230 | 109 | 4 | 5 | 231 | 63 | 290 | 3.02e-77 | 235 |
| MsG0880042313.01.T01 | AT4G14130 | 48.707 | 232 | 111 | 4 | 5 | 231 | 56 | 284 | 2.85e-73 | 224 |
| MsG0880042313.01.T01 | AT1G11545 | 48.291 | 234 | 112 | 5 | 6 | 231 | 67 | 299 | 1.03e-71 | 221 |
| MsG0880042313.01.T01 | AT4G03210 | 44.872 | 234 | 126 | 3 | 3 | 233 | 25 | 258 | 1.18e-71 | 219 |
| MsG0880042313.01.T01 | AT5G57550 | 46.930 | 228 | 115 | 3 | 8 | 233 | 61 | 284 | 4.01e-71 | 219 |
| MsG0880042313.01.T01 | AT4G03210 | 45.415 | 229 | 122 | 3 | 8 | 233 | 58 | 286 | 5.25e-71 | 219 |
| MsG0880042313.01.T01 | AT5G57540 | 49.565 | 230 | 105 | 5 | 8 | 231 | 57 | 281 | 9.17e-71 | 218 |
| MsG0880042313.01.T01 | AT5G57530 | 50.000 | 230 | 104 | 6 | 8 | 231 | 58 | 282 | 1.01e-70 | 218 |
| MsG0880042313.01.T01 | AT3G23730 | 46.725 | 229 | 117 | 3 | 8 | 231 | 58 | 286 | 2.08e-70 | 217 |
| MsG0880042313.01.T01 | AT4G25810 | 48.261 | 230 | 112 | 4 | 8 | 232 | 57 | 284 | 9.04e-69 | 213 |
| MsG0880042313.01.T01 | AT2G14620 | 47.391 | 230 | 114 | 4 | 7 | 231 | 17 | 244 | 3.91e-68 | 210 |
| MsG0880042313.01.T01 | AT4G30280 | 46.903 | 226 | 113 | 4 | 8 | 231 | 61 | 281 | 4.36e-68 | 211 |
| MsG0880042313.01.T01 | AT2G14620 | 47.414 | 232 | 115 | 4 | 5 | 231 | 65 | 294 | 1.20e-67 | 211 |
| MsG0880042313.01.T01 | AT4G28850 | 44.255 | 235 | 121 | 4 | 5 | 231 | 55 | 287 | 1.45e-67 | 210 |
| MsG0880042313.01.T01 | AT4G30270 | 45.575 | 226 | 106 | 4 | 8 | 231 | 55 | 265 | 1.55e-66 | 207 |
| MsG0880042313.01.T01 | AT2G14620 | 48.198 | 222 | 108 | 4 | 15 | 231 | 14 | 233 | 2.68e-66 | 205 |
| MsG0880042313.01.T01 | AT4G25820 | 47.391 | 230 | 110 | 4 | 8 | 231 | 61 | 285 | 2.97e-66 | 206 |
| MsG0880042313.01.T01 | AT1G65310 | 46.460 | 226 | 114 | 4 | 8 | 231 | 61 | 281 | 3.62e-66 | 206 |
| MsG0880042313.01.T01 | AT1G65310 | 46.460 | 226 | 114 | 4 | 8 | 231 | 83 | 303 | 4.50e-66 | 207 |
| MsG0880042313.01.T01 | AT5G57560 | 45.652 | 230 | 119 | 4 | 8 | 232 | 54 | 282 | 1.07e-65 | 205 |
| MsG0880042313.01.T01 | AT4G30290 | 46.087 | 230 | 109 | 6 | 8 | 231 | 56 | 276 | 6.11e-65 | 203 |
| MsG0880042313.01.T01 | AT3G48580 | 45.333 | 225 | 107 | 5 | 8 | 231 | 5 | 214 | 1.10e-64 | 200 |
| MsG0880042313.01.T01 | AT3G48580 | 45.175 | 228 | 109 | 5 | 5 | 231 | 61 | 273 | 1.89e-64 | 202 |
| MsG0880042313.01.T01 | AT5G48070 | 44.248 | 226 | 119 | 4 | 8 | 231 | 61 | 281 | 1.26e-63 | 200 |
| MsG0880042313.01.T01 | AT2G18800 | 42.213 | 244 | 121 | 4 | 5 | 231 | 56 | 296 | 3.71e-63 | 199 |
| MsG0880042313.01.T01 | AT1G32170 | 40.773 | 233 | 129 | 4 | 8 | 232 | 63 | 294 | 7.56e-61 | 194 |
| MsG0880042313.01.T01 | AT1G14720 | 40.171 | 234 | 133 | 3 | 5 | 232 | 59 | 291 | 3.17e-57 | 185 |
| MsG0880042313.01.T01 | AT2G01850 | 40.426 | 235 | 131 | 5 | 5 | 232 | 59 | 291 | 1.57e-55 | 181 |
| MsG0880042313.01.T01 | AT4G18990 | 37.705 | 244 | 132 | 5 | 8 | 232 | 71 | 313 | 4.50e-54 | 177 |
| MsG0880042313.01.T01 | AT1G10550 | 36.214 | 243 | 137 | 4 | 5 | 231 | 69 | 309 | 1.63e-51 | 169 |
| MsG0880042313.01.T01 | AT3G44990 | 41.702 | 235 | 123 | 5 | 5 | 231 | 65 | 293 | 1.64e-51 | 169 |
| MsG0880042313.01.T01 | AT3G48580 | 39.474 | 228 | 102 | 6 | 5 | 231 | 61 | 253 | 9.75e-48 | 158 |
| MsG0880042313.01.T01 | AT4G18990 | 35.498 | 231 | 125 | 5 | 26 | 232 | 9 | 239 | 2.99e-46 | 155 |
| MsG0880042313.01.T01 | AT2G36870 | 39.655 | 232 | 126 | 4 | 8 | 231 | 72 | 297 | 5.65e-46 | 155 |
| MsG0880042313.01.T01 | AT2G36870 | 50.467 | 107 | 45 | 2 | 8 | 106 | 72 | 178 | 8.57e-26 | 99.4 |
Find 60 sgRNAs with CRISPR-Local
Find 117 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATATGTCCAATTTATTT+TGG | 0.159522 | 8:+6975665 | None:intergenic |
| CTTTCAATGTATACATTCTA+AGG | 0.188464 | 8:+6975434 | None:intergenic |
| TTCATGACTACAAAATTCTT+TGG | 0.231926 | 8:-6976672 | MsG0880042313.01.T01:CDS |
| TTTGCATCCAAGTTAAATTA+TGG | 0.253940 | 8:-6977274 | MsG0880042313.01.T01:CDS |
| ATGCCAACTATACAAAATAT+AGG | 0.273762 | 8:-6977304 | MsG0880042313.01.T01:CDS |
| TTGTTCTTGTATACCCTAAT+GGG | 0.301108 | 8:+6975780 | None:intergenic |
| GAGGGAAAGCCAAAATAAAT+TGG | 0.306404 | 8:-6975674 | MsG0880042313.01.T01:CDS |
| TGTACTTGCATTGCCTTTGA+TGG | 0.310019 | 8:+6975735 | None:intergenic |
| ATTGTTCTTGTATACCCTAA+TGG | 0.310320 | 8:+6975779 | None:intergenic |
| ATGTGGACAATATCCCCATT+AGG | 0.314697 | 8:-6975794 | MsG0880042313.01.T01:CDS |
| ATTGTGCTTCTAATAACTAT+TGG | 0.362782 | 8:-6975575 | MsG0880042313.01.T01:CDS |
| ACATTGGTTTGTAATGCATA+TGG | 0.365083 | 8:+6976760 | None:intergenic |
| TGTGGACAATATCCCCATTA+GGG | 0.370292 | 8:-6975793 | MsG0880042313.01.T01:CDS |
| TTCCAAGGATTTGATGTTAG+TGG | 0.377872 | 8:-6975627 | MsG0880042313.01.T01:CDS |
| ACCCCTGCAGAGTCTCTATC+TGG | 0.388277 | 8:+6977220 | None:intergenic |
| CAAACCAATGTATGGGCAAA+TGG | 0.400595 | 8:-6976748 | MsG0880042313.01.T01:CDS |
| GCTCTGGATTATTTCATATG+AGG | 0.402001 | 8:-6977252 | MsG0880042313.01.T01:CDS |
| ATGCATTACAAACCAATGTA+TGG | 0.402005 | 8:-6976756 | MsG0880042313.01.T01:CDS |
| TCCAAGTTAAATTATGGCTC+TGG | 0.420489 | 8:-6977268 | MsG0880042313.01.T01:CDS |
| TGCCAACTATACAAAATATA+GGG | 0.430574 | 8:-6977303 | MsG0880042313.01.T01:CDS |
| TGCAAGTACAAGCAAGTTTG+TGG | 0.438415 | 8:-6975722 | MsG0880042313.01.T01:CDS |
| GTTTGTGGAATGGTGAAGAT+TGG | 0.454151 | 8:-6975707 | MsG0880042313.01.T01:CDS |
| CCTTGGAAGTTTGCTTTGAA+AGG | 0.455209 | 8:+6975642 | None:intergenic |
| TCCAGAGCCATAATTTAACT+TGG | 0.456008 | 8:+6977267 | None:intergenic |
| GTACTTGCATTGCCTTTGAT+GGG | 0.460810 | 8:+6975736 | None:intergenic |
| CATATGACTATTGTAAAGAT+AGG | 0.467990 | 8:-6975476 | MsG0880042313.01.T01:CDS |
| ATCCACTAACATCAAATCCT+TGG | 0.474638 | 8:+6975625 | None:intergenic |
| ACTATACAAAATATAGGGTC+AGG | 0.475617 | 8:-6977298 | MsG0880042313.01.T01:CDS |
| TTCACCATTTGCCCATACAT+TGG | 0.499725 | 8:+6976744 | None:intergenic |
| TAGTCATGAAAATCAGTTGT+GGG | 0.504356 | 8:+6976685 | None:intergenic |
| TCATATTGTCTTTGTCCGGC+AGG | 0.506162 | 8:+6975522 | None:intergenic |
| CCTTTCAAAGCAAACTTCCA+AGG | 0.512461 | 8:-6975642 | MsG0880042313.01.T01:CDS |
| TACAAGAACAATAGCAACAT+TGG | 0.519147 | 8:-6975768 | MsG0880042313.01.T01:CDS |
| GTGCTTCTAATAACTATTGG+TGG | 0.528028 | 8:-6975572 | MsG0880042313.01.T01:CDS |
| TTTGTGGAATGGTGAAGATT+GGG | 0.531318 | 8:-6975706 | MsG0880042313.01.T01:CDS |
| TTTCAATGTATACATTCTAA+GGG | 0.531335 | 8:+6975435 | None:intergenic |
| GTTCCAGATAGAGACTCTGC+AGG | 0.544617 | 8:-6977223 | MsG0880042313.01.T01:CDS |
| TATCTTACACAATTTGATGT+GGG | 0.552342 | 8:+6976647 | None:intergenic |
| ATGTATGGGCAAATGGTGAA+GGG | 0.553560 | 8:-6976741 | MsG0880042313.01.T01:CDS |
| TGCATTACAAACCAATGTAT+GGG | 0.554053 | 8:-6976755 | MsG0880042313.01.T01:CDS |
| AACAATAGCAACATTGGAGT+GGG | 0.554282 | 8:-6975762 | MsG0880042313.01.T01:CDS |
| AATGTATGGGCAAATGGTGA+AGG | 0.554946 | 8:-6976742 | MsG0880042313.01.T01:CDS |
| ACTATTGTAAAGATAGGCAT+AGG | 0.555386 | 8:-6975470 | MsG0880042313.01.T01:CDS |
| GGTGAAGATTGGGCAACAGA+TGG | 0.556325 | 8:-6975696 | MsG0880042313.01.T01:CDS |
| GAAGATTGGGCAACAGATGG+AGG | 0.563488 | 8:-6975693 | MsG0880042313.01.T01:CDS |
| ATACATTCTAAGGGAGGTGT+AGG | 0.563804 | 8:+6975444 | None:intergenic |
| ATATCTTACACAATTTGATG+TGG | 0.570997 | 8:+6976646 | None:intergenic |
| TTGTGGGATCAAACCAAAGG+TGG | 0.606032 | 8:+6976701 | None:intergenic |
| TTCCAGATAGAGACTCTGCA+GGG | 0.612505 | 8:-6977222 | MsG0880042313.01.T01:CDS |
| GTACAAGCAAGTTTGTGGAA+TGG | 0.616101 | 8:-6975717 | MsG0880042313.01.T01:CDS |
| GAACAATAGCAACATTGGAG+TGG | 0.623970 | 8:-6975763 | MsG0880042313.01.T01:CDS |
| CAATGTATACATTCTAAGGG+AGG | 0.625487 | 8:+6975438 | None:intergenic |
| AAGATTGGGCAACAGATGGA+GGG | 0.630194 | 8:-6975692 | MsG0880042313.01.T01:CDS |
| TGTTTGCAGCTGATATCACA+AGG | 0.639360 | 8:-6976835 | MsG0880042313.01.T01:intron |
| TGTATGGGCAAATGGTGAAG+GGG | 0.641157 | 8:-6976740 | MsG0880042313.01.T01:CDS |
| TGTTCTTGTATACCCTAATG+GGG | 0.641328 | 8:+6975781 | None:intergenic |
| GTAGTCATGAAAATCAGTTG+TGG | 0.643007 | 8:+6976684 | None:intergenic |
| TGGAGTGGGATACCCATCAA+AGG | 0.651125 | 8:-6975748 | MsG0880042313.01.T01:CDS |
| CAGTTGTGGGATCAAACCAA+AGG | 0.659281 | 8:+6976698 | None:intergenic |
| TCCAGATAGAGACTCTGCAG+GGG | 0.724270 | 8:-6977221 | MsG0880042313.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAAGTTATCAATTTTATT+TGG | + | Chr8:6976635-6976654 | None:intergenic | 10.0% |
| !!! | CTACAAAAAAATATTTTATT+TGG | + | Chr8:6976607-6976626 | None:intergenic | 10.0% |
| !! | ACCCATAATATAATTTATAA+AGG | - | Chr8:6976778-6976797 | MsG0880042313.01.T01:CDS | 15.0% |
| !! | CCCATAATATAATTTATAAA+GGG | - | Chr8:6976779-6976798 | MsG0880042313.01.T01:CDS | 15.0% |
| !! | CCCTTTATAAATTATATTAT+GGG | + | Chr8:6976782-6976801 | None:intergenic | 15.0% |
| !! | TCCCTTTATAAATTATATTA+TGG | + | Chr8:6976783-6976802 | None:intergenic | 15.0% |
| !!! | TAATTATTTACTTTTGAAAG+TGG | + | Chr8:6976870-6976889 | None:intergenic | 15.0% |
| !! | AAAGACAATTGATTACAAAA+AGG | + | Chr8:6975644-6975663 | None:intergenic | 20.0% |
| !! | AAGAACTAAAATTGATTGAA+AGG | + | Chr8:6976186-6976205 | None:intergenic | 20.0% |
| !! | TATTAATGACATTATTTGCA+GGG | + | Chr8:6975801-6975820 | None:intergenic | 20.0% |
| !! | TGTAATCAATTGTCTTTAAT+AGG | - | Chr8:6975647-6975666 | MsG0880042313.01.T01:CDS | 20.0% |
| !! | TTATTAATGACATTATTTGC+AGG | + | Chr8:6975802-6975821 | None:intergenic | 20.0% |
| !! | TTTCAAATCTGATTAAAACT+TGG | - | Chr8:6976911-6976930 | MsG0880042313.01.T01:intron | 20.0% |
| !!! | AATCATTCATGTTCTTTTTA+AGG | + | Chr8:6976294-6976313 | None:intergenic | 20.0% |
| !!! | ATTTTTCATCGATCTTTTTT+GGG | - | Chr8:6975711-6975730 | MsG0880042313.01.T01:CDS | 20.0% |
| !!! | TATTTTTCATCGATCTTTTT+TGG | - | Chr8:6975710-6975729 | MsG0880042313.01.T01:CDS | 20.0% |
| ! | ACTGGAAAGTAAATAAAATC+TGG | - | Chr8:6976446-6976465 | MsG0880042313.01.T01:intron | 25.0% |
| ! | AGATTCACACTTCTAAATAT+GGG | - | Chr8:6975752-6975771 | MsG0880042313.01.T01:CDS | 25.0% |
| ! | ATATCTTACACAATTTGATG+TGG | + | Chr8:6976098-6976117 | None:intergenic | 25.0% |
| ! | ATGCCAACTATACAAAATAT+AGG | - | Chr8:6975437-6975456 | MsG0880042313.01.T01:CDS | 25.0% |
| ! | ATTAATGACATTATTTGCAG+GGG | + | Chr8:6975800-6975819 | None:intergenic | 25.0% |
| ! | ATTCTCATTTCATGCTATAA+TGG | - | Chr8:6975609-6975628 | MsG0880042313.01.T01:CDS | 25.0% |
| ! | ATTGTGCTTCTAATAACTAT+TGG | - | Chr8:6977166-6977185 | MsG0880042313.01.T01:intron | 25.0% |
| ! | CATATAGTTCCTTCAAAATA+AGG | + | Chr8:6975576-6975595 | None:intergenic | 25.0% |
| ! | CATATGACTATTGTAAAGAT+AGG | - | Chr8:6977265-6977284 | MsG0880042313.01.T01:CDS | 25.0% |
| ! | TAGATTCACACTTCTAAATA+TGG | - | Chr8:6975751-6975770 | MsG0880042313.01.T01:CDS | 25.0% |
| ! | TATCTTACACAATTTGATGT+GGG | + | Chr8:6976097-6976116 | None:intergenic | 25.0% |
| ! | TGCCAACTATACAAAATATA+GGG | - | Chr8:6975438-6975457 | MsG0880042313.01.T01:CDS | 25.0% |
| ! | TGTTTCAAAATCACCAAATT+GGG | + | Chr8:6976239-6976258 | None:intergenic | 25.0% |
| ! | TTCATGACTACAAAATTCTT+TGG | - | Chr8:6976069-6976088 | MsG0880042313.01.T01:intron | 25.0% |
| ! | TTCTCATTTCATGCTATAAT+GGG | - | Chr8:6975610-6975629 | MsG0880042313.01.T01:CDS | 25.0% |
| ! | TTGTTTCAAAATCACCAAAT+TGG | + | Chr8:6976240-6976259 | None:intergenic | 25.0% |
| ! | TTTGCATCCAAGTTAAATTA+TGG | - | Chr8:6975467-6975486 | MsG0880042313.01.T01:CDS | 25.0% |
| !! | ATTTTCATATTGTCTTTGTC+CGG | + | Chr8:6977226-6977245 | None:intergenic | 25.0% |
| !! | GACATCATTTTATCAAGTAT+AGG | + | Chr8:6976345-6976364 | None:intergenic | 25.0% |
| !! | GAGTTAGACTTTGAATTTTT+GGG | - | Chr8:6975942-6975961 | MsG0880042313.01.T01:intron | 25.0% |
| !! | TAGAAGTGTGAATCTAATTA+CGG | + | Chr8:6975747-6975766 | None:intergenic | 25.0% |
| !! | TGCATATGTCCAATTTATTT+TGG | + | Chr8:6977079-6977098 | None:intergenic | 25.0% |
| !! | TGTAGCTCAATTGATAATTA+TGG | + | Chr8:6975826-6975845 | None:intergenic | 25.0% |
| !!! | AAAAAACGTTGATGATGTTT+AGG | - | Chr8:6976212-6976231 | MsG0880042313.01.T01:intron | 25.0% |
| !!! | AGAGGTATGTTTTAAATTCT+TGG | + | Chr8:6976472-6976491 | None:intergenic | 25.0% |
| AATAAGTAACAGACTCTAAG+AGG | + | Chr8:6976490-6976509 | None:intergenic | 30.0% | |
| AATCTGATTAAAACTTGGCT+TGG | - | Chr8:6976916-6976935 | MsG0880042313.01.T01:intron | 30.0% | |
| ACTATACAAAATATAGGGTC+AGG | - | Chr8:6975443-6975462 | MsG0880042313.01.T01:CDS | 30.0% | |
| ACTATTGTAAAGATAGGCAT+AGG | - | Chr8:6977271-6977290 | MsG0880042313.01.T01:CDS | 30.0% | |
| ATGCATTACAAACCAATGTA+TGG | - | Chr8:6975985-6976004 | MsG0880042313.01.T01:intron | 30.0% | |
| ATGTATATGAAGAGTCAAAG+GGG | + | Chr8:6976128-6976147 | None:intergenic | 30.0% | |
| ATTGTTCTTGTATACCCTAA+TGG | + | Chr8:6976965-6976984 | None:intergenic | 30.0% | |
| CATGTATATGAAGAGTCAAA+GGG | + | Chr8:6976129-6976148 | None:intergenic | 30.0% | |
| GTTTCAAAATCACCAAATTG+GGG | + | Chr8:6976238-6976257 | None:intergenic | 30.0% | |
| TACAAGAACAATAGCAACAT+TGG | - | Chr8:6976973-6976992 | MsG0880042313.01.T01:intron | 30.0% | |
| TAGTCATGAAAATCAGTTGT+GGG | + | Chr8:6976059-6976078 | None:intergenic | 30.0% | |
| TCTCATTTCATGCTATAATG+GGG | - | Chr8:6975611-6975630 | MsG0880042313.01.T01:CDS | 30.0% | |
| TGCATTACAAACCAATGTAT+GGG | - | Chr8:6975986-6976005 | MsG0880042313.01.T01:intron | 30.0% | |
| TTGTTCAAAAAATGAGACTG+CGG | + | Chr8:6975677-6975696 | None:intergenic | 30.0% | |
| TTGTTCTTGTATACCCTAAT+GGG | + | Chr8:6976964-6976983 | None:intergenic | 30.0% | |
| ! | ATCTCTTTTTGTTAACGCTA+CGG | - | Chr8:6976387-6976406 | MsG0880042313.01.T01:intron | 30.0% |
| ! | CGAGTTAGACTTTGAATTTT+TGG | - | Chr8:6975941-6975960 | MsG0880042313.01.T01:intron | 30.0% |
| ! | GACCCTATATTTTGTATAGT+TGG | + | Chr8:6975443-6975462 | None:intergenic | 30.0% |
| !! | ACATTGGTTTGTAATGCATA+TGG | + | Chr8:6975984-6976003 | None:intergenic | 30.0% |
| !! | TGACAAAAAGTTTTGGCAAT+TGG | - | Chr8:6977194-6977213 | MsG0880042313.01.T01:intron | 30.0% |
| !!! | GTTCTTTCTCCTTATTTTGA+AGG | - | Chr8:6975564-6975583 | MsG0880042313.01.T01:CDS | 30.0% |
| AACAATAGCAACATTGGAGT+GGG | - | Chr8:6976979-6976998 | MsG0880042313.01.T01:intron | 35.0% | |
| ACAGGAATACGAAATTTGAC+TGG | - | Chr8:6976428-6976447 | MsG0880042313.01.T01:intron | 35.0% | |
| AGTCAAATTTCGTATTCCTG+TGG | + | Chr8:6976429-6976448 | None:intergenic | 35.0% | |
| ATCCACTAACATCAAATCCT+TGG | + | Chr8:6977119-6977138 | None:intergenic | 35.0% | |
| GAGGGAAAGCCAAAATAAAT+TGG | - | Chr8:6977067-6977086 | MsG0880042313.01.T01:intron | 35.0% | |
| GCATGTATATGAAGAGTCAA+AGG | + | Chr8:6976130-6976149 | None:intergenic | 35.0% | |
| GTAGTCATGAAAATCAGTTG+TGG | + | Chr8:6976060-6976079 | None:intergenic | 35.0% | |
| GTGCTTCTAATAACTATTGG+TGG | - | Chr8:6977169-6977188 | MsG0880042313.01.T01:intron | 35.0% | |
| TCCAAGTTAAATTATGGCTC+TGG | - | Chr8:6975473-6975492 | MsG0880042313.01.T01:CDS | 35.0% | |
| TCCAGAGCCATAATTTAACT+TGG | + | Chr8:6975477-6975496 | None:intergenic | 35.0% | |
| TGACTCTTCATATACATGCA+TGG | - | Chr8:6976131-6976150 | MsG0880042313.01.T01:intron | 35.0% | |
| TGTTCTTGTATACCCTAATG+GGG | + | Chr8:6976963-6976982 | None:intergenic | 35.0% | |
| TTCCAAGGATTTGATGTTAG+TGG | - | Chr8:6977114-6977133 | MsG0880042313.01.T01:intron | 35.0% | |
| TTTGTGGAATGGTGAAGATT+GGG | - | Chr8:6977035-6977054 | MsG0880042313.01.T01:intron | 35.0% | |
| ! | CAATGTATACATTCTAAGGG+AGG | + | Chr8:6977306-6977325 | None:intergenic | 35.0% |
| ! | GCTCTGGATTATTTCATATG+AGG | - | Chr8:6975489-6975508 | MsG0880042313.01.T01:CDS | 35.0% |
| ! | GGTGGAATGACAAAAAGTTT+TGG | - | Chr8:6977187-6977206 | MsG0880042313.01.T01:intron | 35.0% |
| ! | TATACATGCATGGTTCTGTT+TGG | - | Chr8:6976141-6976160 | MsG0880042313.01.T01:intron | 35.0% |
| !! | GAATTTTTGGGCAATAGAGA+AGG | - | Chr8:6975954-6975973 | MsG0880042313.01.T01:intron | 35.0% |
| AATGTATGGGCAAATGGTGA+AGG | - | Chr8:6975999-6976018 | MsG0880042313.01.T01:intron | 40.0% | |
| ATGATGTTTAGGCCCCAATT+TGG | - | Chr8:6976223-6976242 | MsG0880042313.01.T01:intron | 40.0% | |
| ATGTATGGGCAAATGGTGAA+GGG | - | Chr8:6976000-6976019 | MsG0880042313.01.T01:intron | 40.0% | |
| ATGTGGACAATATCCCCATT+AGG | - | Chr8:6976947-6976966 | MsG0880042313.01.T01:intron | 40.0% | |
| CAAACCAATGTATGGGCAAA+TGG | - | Chr8:6975993-6976012 | MsG0880042313.01.T01:intron | 40.0% | |
| CCTTTCAAAGCAAACTTCCA+AGG | - | Chr8:6977099-6977118 | MsG0880042313.01.T01:intron | 40.0% | |
| GAACAATAGCAACATTGGAG+TGG | - | Chr8:6976978-6976997 | MsG0880042313.01.T01:intron | 40.0% | |
| GAGAGCAAAAAATCCACCTT+TGG | - | Chr8:6976027-6976046 | MsG0880042313.01.T01:intron | 40.0% | |
| GTACAAGCAAGTTTGTGGAA+TGG | - | Chr8:6977024-6977043 | MsG0880042313.01.T01:intron | 40.0% | |
| GTACTTGCATTGCCTTTGAT+GGG | + | Chr8:6977008-6977027 | None:intergenic | 40.0% | |
| GTTTGTGGAATGGTGAAGAT+TGG | - | Chr8:6977034-6977053 | MsG0880042313.01.T01:intron | 40.0% | |
| TGCAAGTACAAGCAAGTTTG+TGG | - | Chr8:6977019-6977038 | MsG0880042313.01.T01:intron | 40.0% | |
| TGTACTTGCATTGCCTTTGA+TGG | + | Chr8:6977009-6977028 | None:intergenic | 40.0% | |
| TGTGGACAATATCCCCATTA+GGG | - | Chr8:6976948-6976967 | MsG0880042313.01.T01:intron | 40.0% | |
| TGTTTGCAGCTGATATCACA+AGG | - | Chr8:6975906-6975925 | MsG0880042313.01.T01:intron | 40.0% | |
| TTCACCATTTGCCCATACAT+TGG | + | Chr8:6976000-6976019 | None:intergenic | 40.0% | |
| ! | ATACATTCTAAGGGAGGTGT+AGG | + | Chr8:6977300-6977319 | None:intergenic | 40.0% |
| ! | CCTTGGAAGTTTGCTTTGAA+AGG | + | Chr8:6977102-6977121 | None:intergenic | 40.0% |
| !!! | TTGGCTTGGCAGATTTTATG+TGG | - | Chr8:6976930-6976949 | MsG0880042313.01.T01:intron | 40.0% |
| AAGATTGGGCAACAGATGGA+GGG | - | Chr8:6977049-6977068 | MsG0880042313.01.T01:intron | 45.0% | |
| CAGTTGTGGGATCAAACCAA+AGG | + | Chr8:6976046-6976065 | None:intergenic | 45.0% | |
| TCATATTGTCTTTGTCCGGC+AGG | + | Chr8:6977222-6977241 | None:intergenic | 45.0% | |
| TGTATGGGCAAATGGTGAAG+GGG | - | Chr8:6976001-6976020 | MsG0880042313.01.T01:intron | 45.0% | |
| TTCCAGATAGAGACTCTGCA+GGG | - | Chr8:6975519-6975538 | MsG0880042313.01.T01:CDS | 45.0% | |
| TTGTGGGATCAAACCAAAGG+TGG | + | Chr8:6976043-6976062 | None:intergenic | 45.0% | |
| TTGTTAACGCTACGGTTCCT+AGG | - | Chr8:6976395-6976414 | MsG0880042313.01.T01:intron | 45.0% | |
| ! | TTTTGGCAATTGGATCCTGC+CGG | - | Chr8:6977204-6977223 | MsG0880042313.01.T01:CDS | 45.0% |
| GAAGATTGGGCAACAGATGG+AGG | - | Chr8:6977048-6977067 | MsG0880042313.01.T01:intron | 50.0% | |
| GCTACGGTTCCTAGGAGAAA+AGG | - | Chr8:6976403-6976422 | MsG0880042313.01.T01:intron | 50.0% | |
| GGTGAAGATTGGGCAACAGA+TGG | - | Chr8:6977045-6977064 | MsG0880042313.01.T01:intron | 50.0% | |
| GTTCCAGATAGAGACTCTGC+AGG | - | Chr8:6975518-6975537 | MsG0880042313.01.T01:CDS | 50.0% | |
| TCCAGATAGAGACTCTGCAG+GGG | - | Chr8:6975520-6975539 | MsG0880042313.01.T01:CDS | 50.0% | |
| TGGAGTGGGATACCCATCAA+AGG | - | Chr8:6976993-6977012 | MsG0880042313.01.T01:intron | 50.0% | |
| TTCCTAGGAGAAAAGGCCAC+AGG | - | Chr8:6976410-6976429 | MsG0880042313.01.T01:intron | 50.0% | |
| ! | TTCCTGTGGCCTTTTCTCCT+AGG | + | Chr8:6976415-6976434 | None:intergenic | 50.0% |
| ACCCCTGCAGAGTCTCTATC+TGG | + | Chr8:6975524-6975543 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 6975437 | 6977326 | 6975437 | ID=MsG0880042313.01;Name=MsG0880042313.01 |
| Chr8 | mRNA | 6975437 | 6977326 | 6975437 | ID=MsG0880042313.01.T01;Parent=MsG0880042313.01;Name=MsG0880042313.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|1|1|3|0|233 |
| Chr8 | exon | 6977204 | 6977326 | 6977204 | ID=MsG0880042313.01.T01:exon:1895;Parent=MsG0880042313.01.T01 |
| Chr8 | exon | 6976655 | 6976848 | 6976655 | ID=MsG0880042313.01.T01:exon:1894;Parent=MsG0880042313.01.T01 |
| Chr8 | exon | 6975437 | 6975821 | 6975437 | ID=MsG0880042313.01.T01:exon:1893;Parent=MsG0880042313.01.T01 |
| Chr8 | CDS | 6977204 | 6977326 | 6977204 | ID=MsG0880042313.01.T01:cds;Parent=MsG0880042313.01.T01 |
| Chr8 | CDS | 6976655 | 6976848 | 6976655 | ID=MsG0880042313.01.T01:cds;Parent=MsG0880042313.01.T01 |
| Chr8 | CDS | 6975437 | 6975821 | 6975437 | ID=MsG0880042313.01.T01:cds;Parent=MsG0880042313.01.T01 |
| Gene Sequence |
| Protein sequence |
