AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023915.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023915.01.T01 MTR_8g104850 89.835 423 12 1 1 392 64 486 0.0 780
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023915.01.T01 AT4G17430 60.142 424 136 4 1 392 84 506 4.89e-180 510

Find 78 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGTTGTTCCTCCTATTT+TGG 0.107509 4:+91226830 MsG0480023915.01.T01:CDS
CCAATTCTAGAATCTGAATT+TGG 0.165263 4:-91228633 None:intergenic
ATAGAACTAATGAGAAAATT+TGG 0.234217 4:+91228774 MsG0480023915.01.T01:CDS
CATCATAAGGTTACATTTGA+TGG 0.242868 4:+91228390 MsG0480023915.01.T01:CDS
TTGTGCACAGCTTAGATTAC+TGG 0.258292 4:+91228350 MsG0480023915.01.T01:CDS
CTGATCTTCCAAGAAATAAT+TGG 0.261865 4:+91228478 MsG0480023915.01.T01:CDS
CAATGTGGATCCTTGCTATC+AGG 0.262684 4:+91227927 MsG0480023915.01.T01:CDS
GAAACAGATGACTGTGACTT+TGG 0.274790 4:-91227891 None:intergenic
GGAGACGGAGGGATGTATTT+AGG 0.282539 4:+91228105 MsG0480023915.01.T01:CDS
CCAAATTCAGATTCTAGAAT+TGG 0.289157 4:+91228633 MsG0480023915.01.T01:CDS
GTACCAGTCCAATTATTTCT+TGG 0.316051 4:-91228486 None:intergenic
GCAAGGATCCACATTGCTTT+AGG 0.317868 4:-91227920 None:intergenic
GAAAGAAAATATCGTTTGTT+AGG 0.319130 4:+91228084 MsG0480023915.01.T01:CDS
GATCATCATGCCGTTGCTTT+AGG 0.331348 4:+91226852 MsG0480023915.01.T01:CDS
TGATTGCTGGGATACTTAAT+CGG 0.359529 4:+91226805 MsG0480023915.01.T01:CDS
TCCTTGCTATCAGGGTTTCA+TGG 0.369090 4:+91227936 MsG0480023915.01.T01:CDS
ACGGCATGATGATCCAAAAT+AGG 0.372723 4:-91226843 None:intergenic
AGGTTACATTTGATGGGTTA+AGG 0.375123 4:+91228397 MsG0480023915.01.T01:CDS
AACAGAAAACCGAATATCAT+TGG 0.377523 4:-91226904 None:intergenic
AGCTGATTCAACTTTAGAAC+CGG 0.379518 4:-91228138 None:intergenic
CTCTGCTTCAGCTGTTCCTC+CGG 0.383921 4:-91228062 None:intergenic
ACTTACTTAGGTGATTTAAC+TGG 0.389154 4:+91228507 MsG0480023915.01.T01:CDS
TTGTCGAACCCAATGATATT+CGG 0.391953 4:+91226895 MsG0480023915.01.T01:CDS
TGGATTCAGCAACCAGCTTT+CGG 0.395880 4:+91226764 MsG0480023915.01.T01:CDS
TGGTGATGCACATAACTATA+AGG 0.397657 4:+91228527 MsG0480023915.01.T01:CDS
TCAGCTTCAATGCTAGCATT+TGG 0.398062 4:+91228155 MsG0480023915.01.T01:CDS
GTGTGGCATAAGCTTAGATT+TGG 0.407601 4:+91227854 MsG0480023915.01.T01:CDS
AACTAACCACAGCAGGTTAC+GGG 0.410918 4:+91228598 MsG0480023915.01.T01:CDS
CTAAGCTGTGCACAAAGAAA+TGG 0.412607 4:-91228342 None:intergenic
GTATGCAGTTGTGCATCTCT+TGG 0.425074 4:+91228714 MsG0480023915.01.T01:CDS
GCTTCAGCTGTTCCTCCGGT+TGG 0.429937 4:-91228058 None:intergenic
AATTGGACTGGTACTTACTT+AGG 0.431728 4:+91228495 MsG0480023915.01.T01:CDS
TATGACTCGGAACCTTTGTA+TGG 0.459144 4:-91228194 None:intergenic
GTCCCAGAAGTTATGAATGC+TGG 0.466407 4:+91228288 MsG0480023915.01.T01:intron
AAACTAACCACAGCAGGTTA+CGG 0.467715 4:+91228597 MsG0480023915.01.T01:CDS
GGTTCGACAACACGAAACTT+AGG 0.473426 4:-91226882 None:intergenic
ATCATAAGGTTACATTTGAT+GGG 0.480915 4:+91228391 MsG0480023915.01.T01:CDS
CCTTGCTATCAGGGTTTCAT+GGG 0.481021 4:+91227937 MsG0480023915.01.T01:CDS
CCACAGCAGGTTACGGGCAG+AGG 0.481331 4:+91228604 MsG0480023915.01.T01:CDS
GCAGTTTAAGAATCATCATA+AGG 0.481336 4:+91228377 MsG0480023915.01.T01:CDS
TGGGATACTTAATCGGACTT+TGG 0.485040 4:+91226812 MsG0480023915.01.T01:CDS
TATGTTGGATTCATTCCAAC+CGG 0.485792 4:+91228043 MsG0480023915.01.T01:CDS
AACATCAGGTAATATCTGAT+TGG 0.487648 4:-91228670 None:intergenic
CGTGTTTAAACTCCGAAAGC+TGG 0.491440 4:-91226776 None:intergenic
CTTTGTATGGAGCTGAGAAA+AGG 0.495107 4:-91228181 None:intergenic
TCAACATAAAGAAGAACATC+AGG 0.501823 4:-91228684 None:intergenic
ACAGAAAACCGAATATCATT+GGG 0.505777 4:-91226903 None:intergenic
TTAGAATCTTTGATGCAGAA+AGG 0.510610 4:+91228426 MsG0480023915.01.T01:CDS
TGTTCGAGTTATTGATCTGA+GGG 0.513069 4:+91227815 MsG0480023915.01.T01:CDS
AATGTGGATCCTTGCTATCA+GGG 0.529335 4:+91227928 MsG0480023915.01.T01:CDS
AAATATCGTTTGTTAGGAGA+CGG 0.537513 4:+91228090 MsG0480023915.01.T01:CDS
GTCATCGAACTCCTTCGAGC+TGG 0.541269 4:+91226936 MsG0480023915.01.T01:CDS
CTTCCAAGAAATAATTGGAC+TGG 0.550137 4:+91228483 MsG0480023915.01.T01:CDS
GTATTTAGGACACTTGGTCC+CGG 0.550809 4:+91228119 MsG0480023915.01.T01:CDS
GATATCTATAATTTCAGCAA+TGG 0.563519 4:-91227769 None:intergenic
CCCATGAAACCCTGATAGCA+AGG 0.572344 4:-91227937 None:intergenic
AGAAGTTATGAATGCTGGAA+AGG 0.573874 4:+91228293 MsG0480023915.01.T01:intron
AGGGATGTATTTAGGACACT+TGG 0.574056 4:+91228113 MsG0480023915.01.T01:CDS
CGGTCACGAGGATGGTATGT+TGG 0.575077 4:+91228028 MsG0480023915.01.T01:CDS
TTGTTCGAGTTATTGATCTG+AGG 0.576871 4:+91227814 MsG0480023915.01.T01:CDS
CACAGCTTAGATTACTGGAT+GGG 0.576930 4:+91228355 MsG0480023915.01.T01:CDS
AAACTAACCTTCCAGCTCGA+AGG 0.592750 4:-91226947 None:intergenic
CCTCTGCCCGTAACCTGCTG+TGG 0.592897 4:-91228604 None:intergenic
CATGTAGACGGTCACGAGGA+TGG 0.603319 4:+91228020 MsG0480023915.01.T01:CDS
ATCGTTTGTTAGGAGACGGA+GGG 0.612359 4:+91228094 MsG0480023915.01.T01:CDS
GCACAGCTTAGATTACTGGA+TGG 0.615240 4:+91228354 MsG0480023915.01.T01:CDS
TCGAACTCCTTCGAGCTGGA+AGG 0.617618 4:+91226940 MsG0480023915.01.T01:CDS
TATCGTTTGTTAGGAGACGG+AGG 0.623832 4:+91228093 MsG0480023915.01.T01:CDS
GCATGATGATCCAAAATAGG+AGG 0.624373 4:-91226840 None:intergenic
GCTGATTCAACTTTAGAACC+GGG 0.627014 4:-91228137 None:intergenic
GTTGGATTCATTCCAACCGG+AGG 0.650228 4:+91228046 MsG0480023915.01.T01:CDS
GTGTGGACTTATCATGTAGA+CGG 0.654777 4:+91228008 MsG0480023915.01.T01:CDS
AGGACAACTACCTAAAGCAA+CGG 0.656379 4:-91226862 None:intergenic
TCTGAAAGCCTAAAGCAATG+TGG 0.680337 4:+91227912 MsG0480023915.01.T01:CDS
TTATCATGTAGACGGTCACG+AGG 0.696567 4:+91228016 MsG0480023915.01.T01:CDS
ATGTGGTATGCACCTCACAG+TGG 0.702620 4:+91226744 MsG0480023915.01.T01:CDS
AAGATTGCAGAACTACAGTG+TGG 0.731083 4:+91227991 MsG0480023915.01.T01:CDS
TGGTTGCTGAATCCACTGTG+AGG 0.753140 4:-91226756 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATATAAAAAATTTCTAT+TGG + Chr4:91227421-91227440 MsG0480023915.01.T01:intron 10.0%
!! AATAAGTTAAAAACAACTTA+TGG + Chr4:91227240-91227259 MsG0480023915.01.T01:intron 15.0%
!! ATATGTAATTGAGTTAAAAA+GGG + Chr4:91227456-91227475 MsG0480023915.01.T01:intron 15.0%
!! TATATGTAATTGAGTTAAAA+AGG + Chr4:91227455-91227474 MsG0480023915.01.T01:intron 15.0%
!!! TAAGCTATTTTTATAACAAA+AGG + Chr4:91227146-91227165 MsG0480023915.01.T01:intron 15.0%
!! AAATTAACTCAATTACATCA+CGG - Chr4:91227599-91227618 None:intergenic 20.0%
!! ATAGAACTAATGAGAAAATT+TGG + Chr4:91228774-91228793 MsG0480023915.01.T01:CDS 20.0%
!! ATGAATATCAATATATGACT+CGG - Chr4:91228210-91228229 None:intergenic 20.0%
!!! TGGATTTTCAATTTGAAATT+AGG + Chr4:91227070-91227089 MsG0480023915.01.T01:intron 20.0%
! AATTAACTCAATTACATCAC+GGG - Chr4:91227598-91227617 None:intergenic 25.0%
! AGTCATTACAAAAATGTGTA+TGG - Chr4:91228460-91228479 None:intergenic 25.0%
! ATCATAAGGTTACATTTGAT+GGG + Chr4:91228391-91228410 MsG0480023915.01.T01:CDS 25.0%
! GAAAGAAAATATCGTTTGTT+AGG + Chr4:91228084-91228103 MsG0480023915.01.T01:CDS 25.0%
! GATATCTATAATTTCAGCAA+TGG - Chr4:91227772-91227791 None:intergenic 25.0%
! GTCATTACAAAAATGTGTAT+GGG - Chr4:91228459-91228478 None:intergenic 25.0%
! TGCTATTATTACGAGAAAAA+AGG - Chr4:91227622-91227641 None:intergenic 25.0%
!! AGTCAATTTCTTTTCATGTA+AGG + Chr4:91227177-91227196 MsG0480023915.01.T01:intron 25.0%
!! TTTGAAATTAGGTTTACGTT+TGG + Chr4:91227081-91227100 MsG0480023915.01.T01:intron 25.0%
!!! TAGTTCTATATTTTCAGCAA+TGG - Chr4:91228763-91228782 None:intergenic 25.0%
AACAGAAAACCGAATATCAT+TGG - Chr4:91226907-91226926 None:intergenic 30.0%
AACATCAGGTAATATCTGAT+TGG - Chr4:91228673-91228692 None:intergenic 30.0%
ACAGAAAACCGAATATCATT+GGG - Chr4:91226906-91226925 None:intergenic 30.0%
ACTTACTTAGGTGATTTAAC+TGG + Chr4:91228507-91228526 MsG0480023915.01.T01:CDS 30.0%
AGACTGTTTAGAAGAGATTA+TGG - Chr4:91227288-91227307 None:intergenic 30.0%
CATCATAAGGTTACATTTGA+TGG + Chr4:91228390-91228409 MsG0480023915.01.T01:CDS 30.0%
CTATGTTGTGTGAAAAATCA+TGG - Chr4:91227394-91227413 None:intergenic 30.0%
CTGATCTTCCAAGAAATAAT+TGG + Chr4:91228478-91228497 MsG0480023915.01.T01:CDS 30.0%
CTTAAATAAGCAAATCCAAG+AGG + Chr4:91227334-91227353 MsG0480023915.01.T01:intron 30.0%
GCAGTTTAAGAATCATCATA+AGG + Chr4:91228377-91228396 MsG0480023915.01.T01:CDS 30.0%
TCAACATAAAGAAGAACATC+AGG - Chr4:91228687-91228706 None:intergenic 30.0%
TGATATTCATGAATCTCATC+AGG + Chr4:91228220-91228239 MsG0480023915.01.T01:intron 30.0%
TTAGAATCTTTGATGCAGAA+AGG + Chr4:91228426-91228445 MsG0480023915.01.T01:CDS 30.0%
! AAATATCGTTTGTTAGGAGA+CGG + Chr4:91228090-91228109 MsG0480023915.01.T01:CDS 30.0%
! AACTTCTAAGCTGTGATTTT+AGG + Chr4:91227650-91227669 MsG0480023915.01.T01:intron 30.0%
! ACAAAGAAATGGAGCTTTTA+TGG - Chr4:91228334-91228353 None:intergenic 30.0%
! ACTTCTAAGCTGTGATTTTA+GGG + Chr4:91227651-91227670 MsG0480023915.01.T01:intron 30.0%
! ATGATATTCGGTTTTCTGTT+TGG + Chr4:91226907-91226926 MsG0480023915.01.T01:CDS 30.0%
! TGATATTCGGTTTTCTGTTT+GGG + Chr4:91226908-91226927 MsG0480023915.01.T01:CDS 30.0%
!! CCAAATTCAGATTCTAGAAT+TGG + Chr4:91228633-91228652 MsG0480023915.01.T01:CDS 30.0%
!! CCAATTCTAGAATCTGAATT+TGG - Chr4:91228636-91228655 None:intergenic 30.0%
AACAGCCTCTTGTGTAAAAT+AGG - Chr4:91227562-91227581 None:intergenic 35.0%
ACAGCCTCTTGTGTAAAATA+GGG - Chr4:91227561-91227580 None:intergenic 35.0%
ACGATTCAGTGACTACAAAT+TGG + Chr4:91227050-91227069 MsG0480023915.01.T01:intron 35.0%
AGAAGTTATGAATGCTGGAA+AGG + Chr4:91228293-91228312 MsG0480023915.01.T01:intron 35.0%
CTCTTGTGTAAAATAGGGTA+AGG - Chr4:91227556-91227575 None:intergenic 35.0%
CTTCCAAGAAATAATTGGAC+TGG + Chr4:91228483-91228502 MsG0480023915.01.T01:CDS 35.0%
GTTGAAAAACTGTCGAATTC+TGG + Chr4:91226975-91226994 MsG0480023915.01.T01:intron 35.0%
TATGTTGGATTCATTCCAAC+CGG + Chr4:91228043-91228062 MsG0480023915.01.T01:CDS 35.0%
TGATTGCTGGGATACTTAAT+CGG + Chr4:91226805-91226824 MsG0480023915.01.T01:CDS 35.0%
TTCCAGCATTCATAACTTCT+GGG - Chr4:91228293-91228312 None:intergenic 35.0%
TTGTCGAACCCAATGATATT+CGG + Chr4:91226895-91226914 MsG0480023915.01.T01:CDS 35.0%
TTTCCAGCATTCATAACTTC+TGG - Chr4:91228294-91228313 None:intergenic 35.0%
! CAAAAATGTGTATGGGTTGA+TGG - Chr4:91228452-91228471 None:intergenic 35.0%
! CTTACCCTATTTTACACAAG+AGG + Chr4:91227554-91227573 MsG0480023915.01.T01:intron 35.0%
! GCAGAGGTTTTTATCTTTGA+TGG + Chr4:91228244-91228263 MsG0480023915.01.T01:intron 35.0%
! GTACCAGTCCAATTATTTCT+TGG - Chr4:91228489-91228508 None:intergenic 35.0%
! TGGTGATGCACATAACTATA+AGG + Chr4:91228527-91228546 MsG0480023915.01.T01:CDS 35.0%
! TGTTCGAGTTATTGATCTGA+GGG + Chr4:91227815-91227834 MsG0480023915.01.T01:CDS 35.0%
! TTGTTCGAGTTATTGATCTG+AGG + Chr4:91227814-91227833 MsG0480023915.01.T01:CDS 35.0%
! TTTGGTTGTTCCTCCTATTT+TGG + Chr4:91226830-91226849 MsG0480023915.01.T01:CDS 35.0%
!! AATTGGACTGGTACTTACTT+AGG + Chr4:91228495-91228514 MsG0480023915.01.T01:CDS 35.0%
!! AGCTGATTCAACTTTAGAAC+CGG - Chr4:91228141-91228160 None:intergenic 35.0%
!! AGGTTACATTTGATGGGTTA+AGG + Chr4:91228397-91228416 MsG0480023915.01.T01:CDS 35.0%
!! GATTTTGTTTCGATATGGTG+TGG + Chr4:91227837-91227856 MsG0480023915.01.T01:CDS 35.0%
!! TGAGGGATTTTGTTTCGATA+TGG + Chr4:91227832-91227851 MsG0480023915.01.T01:CDS 35.0%
!!! TGTGCATCTCTTGGTTTTAT+TGG + Chr4:91228723-91228742 MsG0480023915.01.T01:CDS 35.0%
AAACTAACCACAGCAGGTTA+CGG + Chr4:91228597-91228616 MsG0480023915.01.T01:CDS 40.0%
AAGATTGCAGAACTACAGTG+TGG + Chr4:91227991-91228010 MsG0480023915.01.T01:CDS 40.0%
AAGCTAATCGGGAGAGTTTA+TGG + Chr4:91227216-91227235 MsG0480023915.01.T01:intron 40.0%
ACATACCTGCAAGAAAAGAG+TGG - Chr4:91227747-91227766 None:intergenic 40.0%
ACGGCATGATGATCCAAAAT+AGG - Chr4:91226846-91226865 None:intergenic 40.0%
AGGACAACTACCTAAAGCAA+CGG - Chr4:91226865-91226884 None:intergenic 40.0%
CACAGCTTAGATTACTGGAT+GGG + Chr4:91228355-91228374 MsG0480023915.01.T01:CDS 40.0%
CATGTAAACTACACTGCACT+TGG + Chr4:91227694-91227713 MsG0480023915.01.T01:intron 40.0%
CTAAGCTGTGCACAAAGAAA+TGG - Chr4:91228345-91228364 None:intergenic 40.0%
CTTTGTATGGAGCTGAGAAA+AGG - Chr4:91228184-91228203 None:intergenic 40.0%
GAAACAGATGACTGTGACTT+TGG - Chr4:91227894-91227913 None:intergenic 40.0%
GCATGATGATCCAAAATAGG+AGG - Chr4:91226843-91226862 None:intergenic 40.0%
GTGTGGACTTATCATGTAGA+CGG + Chr4:91228008-91228027 MsG0480023915.01.T01:CDS 40.0%
GTGTGGCATAAGCTTAGATT+TGG + Chr4:91227854-91227873 MsG0480023915.01.T01:CDS 40.0%
TCAGCTTCAATGCTAGCATT+TGG + Chr4:91228155-91228174 MsG0480023915.01.T01:CDS 40.0%
TCTGAAAGCCTAAAGCAATG+TGG + Chr4:91227912-91227931 MsG0480023915.01.T01:CDS 40.0%
TGGGATACTTAATCGGACTT+TGG + Chr4:91226812-91226831 MsG0480023915.01.T01:CDS 40.0%
TTGAGTTAAAAAGGGCAACC+CGG + Chr4:91227464-91227483 MsG0480023915.01.T01:intron 40.0%
TTGTGCACAGCTTAGATTAC+TGG + Chr4:91228350-91228369 MsG0480023915.01.T01:CDS 40.0%
! AATGTGGATCCTTGCTATCA+GGG + Chr4:91227928-91227947 MsG0480023915.01.T01:CDS 40.0%
! AGGGATGTATTTAGGACACT+TGG + Chr4:91228113-91228132 MsG0480023915.01.T01:CDS 40.0%
! AGTTGTTTCCGCAAGCTAAT+CGG + Chr4:91227204-91227223 MsG0480023915.01.T01:intron 40.0%
! ATCAGTATCTAGAGTCCTCT+TGG - Chr4:91227352-91227371 None:intergenic 40.0%
! CTTTTCTCAGCTCCATACAA+AGG + Chr4:91228182-91228201 MsG0480023915.01.T01:CDS 40.0%
! TATGACTCGGAACCTTTGTA+TGG - Chr4:91228197-91228216 None:intergenic 40.0%
! TTTTCAGCAATGGTTGATCC+AGG - Chr4:91228753-91228772 None:intergenic 40.0%
!! AAACACGCTCTTTTGATTGC+TGG + Chr4:91226792-91226811 MsG0480023915.01.T01:CDS 40.0%
!! AACACGCTCTTTTGATTGCT+GGG + Chr4:91226793-91226812 MsG0480023915.01.T01:CDS 40.0%
!! CATTCATAACTTCTGGGACA+TGG - Chr4:91228287-91228306 None:intergenic 40.0%
!! GCTGATTCAACTTTAGAACC+GGG - Chr4:91228140-91228159 None:intergenic 40.0%
!!! ACCTGCTGTGGTTAGTTTTT+TGG - Chr4:91228595-91228614 None:intergenic 40.0%
!!! CTTGGTTTTATTGGAACTCC+TGG + Chr4:91228732-91228751 MsG0480023915.01.T01:CDS 40.0%
AAACTAACCTTCCAGCTCGA+AGG - Chr4:91226950-91226969 None:intergenic 45.0%
AACTAACCACAGCAGGTTAC+GGG + Chr4:91228598-91228617 MsG0480023915.01.T01:CDS 45.0%
ACCTGCAAGAAAAGAGTGGA+CGG - Chr4:91227743-91227762 None:intergenic 45.0%
ATGAATCTCATCAGGAGCAG+AGG + Chr4:91228228-91228247 MsG0480023915.01.T01:intron 45.0%
CGTGCAATACACCAAATGGT+GGG - Chr4:91227530-91227549 None:intergenic 45.0%
CGTGTTTAAACTCCGAAAGC+TGG - Chr4:91226779-91226798 None:intergenic 45.0%
CTGTCGAATTCTGGTCAAGT+AGG + Chr4:91226984-91227003 MsG0480023915.01.T01:intron 45.0%
GCAAGGATCCACATTGCTTT+AGG - Chr4:91227923-91227942 None:intergenic 45.0%
GCACAGCTTAGATTACTGGA+TGG + Chr4:91228354-91228373 MsG0480023915.01.T01:CDS 45.0%
GCCAAAAAACTAACCACAGC+AGG + Chr4:91228591-91228610 MsG0480023915.01.T01:CDS 45.0%
GGTTCGACAACACGAAACTT+AGG - Chr4:91226885-91226904 None:intergenic 45.0%
GTATGCAGTTGTGCATCTCT+TGG + Chr4:91228714-91228733 MsG0480023915.01.T01:CDS 45.0%
GTCCCAGAAGTTATGAATGC+TGG + Chr4:91228288-91228307 MsG0480023915.01.T01:intron 45.0%
TGGATTCAGCAACCAGCTTT+CGG + Chr4:91226764-91226783 MsG0480023915.01.T01:CDS 45.0%
TTATCATGTAGACGGTCACG+AGG + Chr4:91228016-91228035 MsG0480023915.01.T01:CDS 45.0%
! ATCGTTTGTTAGGAGACGGA+GGG + Chr4:91228094-91228113 MsG0480023915.01.T01:CDS 45.0%
! CAATGTGGATCCTTGCTATC+AGG + Chr4:91227927-91227946 MsG0480023915.01.T01:CDS 45.0%
! CCTTGCTATCAGGGTTTCAT+GGG + Chr4:91227937-91227956 MsG0480023915.01.T01:CDS 45.0%
! GATCATCATGCCGTTGCTTT+AGG + Chr4:91226852-91226871 MsG0480023915.01.T01:CDS 45.0%
! GTATTTAGGACACTTGGTCC+CGG + Chr4:91228119-91228138 MsG0480023915.01.T01:CDS 45.0%
! GTTGTTTCCGCAAGCTAATC+GGG + Chr4:91227205-91227224 MsG0480023915.01.T01:intron 45.0%
! TATCGTTTGTTAGGAGACGG+AGG + Chr4:91228093-91228112 MsG0480023915.01.T01:CDS 45.0%
! TCCTTGCTATCAGGGTTTCA+TGG + Chr4:91227936-91227955 MsG0480023915.01.T01:CDS 45.0%
! TTACACAAGAGGCTGTTTCC+AGG + Chr4:91227565-91227584 MsG0480023915.01.T01:intron 45.0%
!! AAACTCTCCCGATTAGCTTG+CGG - Chr4:91227215-91227234 None:intergenic 45.0%
ACTAGAGCTCCCACATATGC+AGG + Chr4:91227490-91227509 MsG0480023915.01.T01:intron 50.0%
ATGTGGTATGCACCTCACAG+TGG + Chr4:91226744-91226763 MsG0480023915.01.T01:CDS 50.0%
CCCATGAAACCCTGATAGCA+AGG - Chr4:91227940-91227959 None:intergenic 50.0%
CTAGAGCTCCCACATATGCA+GGG + Chr4:91227491-91227510 MsG0480023915.01.T01:intron 50.0%
GCGTGCAATACACCAAATGG+TGG - Chr4:91227531-91227550 None:intergenic 50.0%
GCTGCGTGCAATACACCAAA+TGG - Chr4:91227534-91227553 None:intergenic 50.0%
GGAGACGGAGGGATGTATTT+AGG + Chr4:91228105-91228124 MsG0480023915.01.T01:CDS 50.0%
GTTGGATTCATTCCAACCGG+AGG + Chr4:91228046-91228065 MsG0480023915.01.T01:CDS 50.0%
TACATCACGGGTTCAAGTCC+TGG - Chr4:91227586-91227605 None:intergenic 50.0%
TGGTTGCTGAATCCACTGTG+AGG - Chr4:91226759-91226778 None:intergenic 50.0%
! TCCGTCCACTCTTTTCTTGC+AGG + Chr4:91227739-91227758 MsG0480023915.01.T01:intron 50.0%
ACATATGCAGGGTCCGGGAA+GGG + Chr4:91227502-91227521 MsG0480023915.01.T01:intron 55.0%
CATGTAGACGGTCACGAGGA+TGG + Chr4:91228020-91228039 MsG0480023915.01.T01:CDS 55.0%
CGGTCACGAGGATGGTATGT+TGG + Chr4:91228028-91228047 MsG0480023915.01.T01:CDS 55.0%
CTCTGCTTCAGCTGTTCCTC+CGG - Chr4:91228065-91228084 None:intergenic 55.0%
GTCATCGAACTCCTTCGAGC+TGG + Chr4:91226936-91226955 MsG0480023915.01.T01:CDS 55.0%
TCGAACTCCTTCGAGCTGGA+AGG + Chr4:91226940-91226959 MsG0480023915.01.T01:CDS 55.0%
TTCCCGGACCCTGCATATGT+GGG - Chr4:91227502-91227521 None:intergenic 55.0%
! ATGTGGGAGCTCTAGTGCAC+CGG - Chr4:91227486-91227505 None:intergenic 55.0%
CACATATGCAGGGTCCGGGA+AGG + Chr4:91227501-91227520 MsG0480023915.01.T01:intron 60.0%
CATATGCAGGGTCCGGGAAG+GGG + Chr4:91227503-91227522 MsG0480023915.01.T01:intron 60.0%
CTCCCACATATGCAGGGTCC+GGG + Chr4:91227497-91227516 MsG0480023915.01.T01:intron 60.0%
CTTCCCGGACCCTGCATATG+TGG - Chr4:91227503-91227522 None:intergenic 60.0%
GCTCCCACATATGCAGGGTC+CGG + Chr4:91227496-91227515 MsG0480023915.01.T01:intron 60.0%
GCTTCAGCTGTTCCTCCGGT+TGG - Chr4:91228061-91228080 None:intergenic 60.0%
! CAAATGGTGGGACCCCTTCC+CGG - Chr4:91227518-91227537 None:intergenic 60.0%
! TGTGGGAGCTCTAGTGCACC+GGG - Chr4:91227485-91227504 None:intergenic 60.0%
CCACAGCAGGTTACGGGCAG+AGG + Chr4:91228604-91228623 MsG0480023915.01.T01:CDS 65.0%
CCTCTGCCCGTAACCTGCTG+TGG - Chr4:91228607-91228626 None:intergenic 65.0%
CGGGAAGGGGTCCCACCATT+TGG + Chr4:91227516-91227535 MsG0480023915.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 91226744 91228812 91226744 ID=MsG0480023915.01;Name=MsG0480023915.01
Chr4 mRNA 91226744 91228812 91226744 ID=MsG0480023915.01.T01;Parent=MsG0480023915.01;Name=MsG0480023915.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|1|1|3|0|392
Chr4 exon 91226744 91226961 91226744 ID=MsG0480023915.01.T01:exon:13216;Parent=MsG0480023915.01.T01
Chr4 exon 91227761 91228203 91227761 ID=MsG0480023915.01.T01:exon:13217;Parent=MsG0480023915.01.T01
Chr4 exon 91228295 91228812 91228295 ID=MsG0480023915.01.T01:exon:13218;Parent=MsG0480023915.01.T01
Chr4 CDS 91226744 91226961 91226744 ID=MsG0480023915.01.T01:cds;Parent=MsG0480023915.01.T01
Chr4 CDS 91227761 91228203 91227761 ID=MsG0480023915.01.T01:cds;Parent=MsG0480023915.01.T01
Chr4 CDS 91228295 91228812 91228295 ID=MsG0480023915.01.T01:cds;Parent=MsG0480023915.01.T01
Gene Sequence

>MsG0480023915.01.T01

ATGTGGTATGCACCTCACAGTGGATTCAGCAACCAGCTTTCGGAGTTTAAACACGCTCTTTTGATTGCTGGGATACTTAATCGGACTTTGGTTGTTCCTCCTATTTTGGATCATCATGCCGTTGCTTTAGGTAGTTGTCCTAAGTTTCGTGTTGTCGAACCCAATGATATTCGGTTTTCTGTTTGGGATCATGTCATCGAACTCCTTCGAGCTGGAAGGTATGTCTCCATTGCTGAAATTATAGATATCTCATCTTTAGTTTCTTCTAGTCTTGTTCGAGTTATTGATCTGAGGGATTTTGTTTCGATATGGTGTGGCATAAGCTTAGATTTGGCTTGTAATAATGATCCAAAGTCACAGTCATCTGTTTCTGAAAGCCTAAAGCAATGTGGATCCTTGCTATCAGGGTTTCATGGGAATATTGAAAAGTGTATATATGCTATCAATGAAGATTGCAGAACTACAGTGTGGACTTATCATGTAGACGGTCACGAGGATGGTATGTTGGATTCATTCCAACCGGAGGAACAGCTGAAGCAGAGAAAGAAAATATCGTTTGTTAGGAGACGGAGGGATGTATTTAGGACACTTGGTCCCGGTTCTAAAGTTGAATCAGCTTCAATGCTAGCATTTGGAAGCCTTTTCTCAGCTCCATACAAAGAAGTTATGAATGCTGGAAAGGAGTTCGCTAAAGCAACCATAAAAGCTCCATTTCTTTGTGCACAGCTTAGATTACTGGATGGGCAGTTTAAGAATCATCATAAGGTTACATTTGATGGGTTAAGGCAAAAATTAGAATCTTTGATGCAGAAAGGCCATCAACCCATACACATTTTTGTAATGACTGATCTTCCAAGAAATAATTGGACTGGTACTTACTTAGGTGATTTAACTGGTGATGCACATAACTATAAGGTGCATTTCTTAAGAGAAGATGATCAGCTAGTTATGCAAGCAGCCAAAAAACTAACCACAGCAGGTTACGGGCAGAGGTTTATTCCAAATTCAGATTCTAGAATTGGTAAAAAGTATTGCTCCAATCAGATATTACCTGATGTTCTTCTTTATGTTGAGCAGACTGTATGCAGTTGTGCATCTCTTGGTTTTATTGGAACTCCTGGATCAACCATTGCTGAAAATATAGAACTAATGAGAAAATTTGGTTCTTGTTCTAGTTGA
Protein sequence

>MsG0480023915.01.T01

MWYAPHSGFSNQLSEFKHALLIAGILNRTLVVPPILDHHAVALGSCPKFRVVEPNDIRFSVWDHVIELLRAGRYVSIAEIIDISSLVSSSLVRVIDLRDFVSIWCGISLDLACNNDPKSQSSVSESLKQCGSLLSGFHGNIEKCIYAINEDCRTTVWTYHVDGHEDGMLDSFQPEEQLKQRKKISFVRRRRDVFRTLGPGSKVESASMLAFGSLFSAPYKEVMNAGKEFAKATIKAPFLCAQLRLLDGQFKNHHKVTFDGLRQKLESLMQKGHQPIHIFVMTDLPRNNWTGTYLGDLTGDAHNYKVHFLREDDQLVMQAAKKLTTAGYGQRFIPNSDSRIGKKYCSNQILPDVLLYVEQTVCSCASLGFIGTPGSTIAENIELMRKFGSCSS*