AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024057.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024057.01.T01 MTR_5g005160 94.297 491 24 1 1 491 1 487 0.0 890
MsG0580024057.01.T01 MTR_5g026780 57.727 440 183 3 13 450 45 483 0.0 517
MsG0580024057.01.T01 MTR_5g026780 57.727 440 183 3 13 450 29 467 0.0 517
MsG0580024057.01.T01 MTR_1g112950 58.372 430 178 1 13 442 25 453 9.61e-180 512
MsG0580024057.01.T01 MTR_0031s0060 53.425 438 179 2 3 440 129 541 3.52e-164 480
MsG0580024057.01.T01 MTR_8g085630 49.436 443 220 3 2 441 28 469 1.44e-142 418
MsG0580024057.01.T01 MTR_5g064750 47.273 440 228 3 13 449 24 462 6.88e-134 395
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024057.01.T01 AT3G19553 72.246 472 125 4 2 469 10 479 0.0 667
MsG0580024057.01.T01 AT1G31830 56.582 433 187 1 8 440 43 474 0.0 521
MsG0580024057.01.T01 AT1G31830 56.582 433 187 1 8 440 27 458 0.0 520
MsG0580024057.01.T01 AT5G05630 56.582 433 185 2 13 443 52 483 0.0 518
MsG0580024057.01.T01 AT1G31820 55.140 428 191 1 13 440 35 461 4.16e-166 478
MsG0580024057.01.T01 AT3G13620 46.548 449 232 5 9 452 25 470 1.44e-130 387

Find 113 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCGCCTTAGTCTGATTT+TGG 0.077741 5:+339350 None:intergenic
CTTTGGATATCCTCTGCTTT+TGG 0.256985 5:-339639 MsG0580024057.01.T01:CDS
GCCCGTATTCCTGCTCTCTT+AGG 0.274297 5:-339492 MsG0580024057.01.T01:CDS
AGAGGTAATAAGGTTAGTTT+TGG 0.281281 5:+339846 None:intergenic
ACAACGGTGGCTATGTTCTT+TGG 0.294038 5:-339656 MsG0580024057.01.T01:CDS
GTTACTTCAATAACATGTTT+TGG 0.298728 5:-339293 MsG0580024057.01.T01:CDS
ATTTATCTTGTACCCTATCT+TGG 0.304077 5:-338273 MsG0580024057.01.T01:CDS
TAGAGCAATCAGAGGTAATA+AGG 0.309669 5:+339836 None:intergenic
CTGAAAGGTCTATAAAGATT+TGG 0.310531 5:+338419 None:intergenic
GAAGTCTCCGGCGGTCCATT+TGG 0.336911 5:-339804 MsG0580024057.01.T01:CDS
TCAGCGGTGATGAGAGCTTC+AGG 0.337698 5:+339699 None:intergenic
ACTTATTCTTGTTATGTGTC+TGG 0.338301 5:-338342 MsG0580024057.01.T01:CDS
TTCTTGGCTTGAACCAAGAT+AGG 0.345537 5:+338260 None:intergenic
GAAATGGTTTAGTGGTGTTA+TGG 0.353410 5:-339586 MsG0580024057.01.T01:CDS
GAATTTGAATTATTGGGATA+AGG 0.366638 5:-339271 MsG0580024057.01.T01:CDS
CTCAATTTCTTATATGCTAT+AGG 0.369168 5:-338488 MsG0580024057.01.T01:CDS
AGAGTTCCGTTCCAAACATT+TGG 0.378355 5:-338398 MsG0580024057.01.T01:CDS
GCAAGGCCGCACCAAATGTT+TGG 0.383110 5:+338387 None:intergenic
CTCTGTAATTCAAATAAGTA+AGG 0.394814 5:+339457 None:intergenic
AAACTTTCTAGGTCTAAGTA+TGG 0.399700 5:-338593 MsG0580024057.01.T01:intron
GATAAGGCTAGTACTCTTGC+AGG 0.406915 5:-339255 MsG0580024057.01.T01:CDS
TGAGTTCCCCTCCAACTGAA+TGG 0.407650 5:-339128 MsG0580024057.01.T01:CDS
TTGAGAACGTTCTTTGTAAG+TGG 0.408514 5:-338314 MsG0580024057.01.T01:CDS
GAGTTCCCCTCCAACTGAAT+GGG 0.414167 5:-339127 MsG0580024057.01.T01:CDS
TACAGAGGTCTTCACATTGT+TGG 0.426683 5:-339441 MsG0580024057.01.T01:CDS
GCATTCAATTGATTCAGAAC+TGG 0.430163 5:-338183 MsG0580024057.01.T01:CDS
ATTCCGTTGCTAGCAGGAAC+AGG 0.431465 5:-339156 MsG0580024057.01.T01:CDS
GCTCAAGTCGGGATGTTGAT+TGG 0.436679 5:-339090 MsG0580024057.01.T01:CDS
GATGAGCTTTCAGCAAATAC+TGG 0.438639 5:-338516 MsG0580024057.01.T01:CDS
ACCTTCTTGAAAACCCCAAA+AGG 0.445470 5:+339614 None:intergenic
ACAGAATCCTCAACTCCAAA+TGG 0.446257 5:+339789 None:intergenic
CAAGACAAGACCCTCTACTT+TGG 0.447099 5:+338068 None:intergenic
TCCCTAAGAGAGCAGGAATA+CGG 0.463074 5:+339490 None:intergenic
CGGCGGTCCATTTGGAGTTG+AGG 0.468150 5:-339796 MsG0580024057.01.T01:CDS
GTTGAGGATTCTGTTAGAAC+CGG 0.475041 5:-339780 MsG0580024057.01.T01:CDS
AAGAACAAAGATAACAAGAA+AGG 0.478885 5:+339380 None:intergenic
CTGTTCCCAAAATCAGACTA+AGG 0.482200 5:-339356 MsG0580024057.01.T01:CDS
CTTCTTGGAATGAGCAAGAT+GGG 0.487711 5:-338976 MsG0580024057.01.T01:CDS
TATGAGCTCACCATTGCCTA+TGG 0.489162 5:-338129 MsG0580024057.01.T01:CDS
CCCGTATTCCTGCTCTCTTA+GGG 0.496526 5:-339491 MsG0580024057.01.T01:CDS
TATTTGATTCCGTTGCTAGC+AGG 0.499339 5:-339162 MsG0580024057.01.T01:CDS
AAGTAATCAAGAAACAAAAC+AGG 0.501951 5:+339549 None:intergenic
GGTCCTCTTCTATCCTTACT+CGG 0.505486 5:-339753 MsG0580024057.01.T01:CDS
GCCGCTGCCATGTCTAACCT+TGG 0.505741 5:-339036 MsG0580024057.01.T01:CDS
ATCAATTGAATGCCAACCAT+TGG 0.506251 5:+338193 None:intergenic
GACCTTGATGAGAATACAGC+TGG 0.509598 5:+338946 None:intergenic
TTCCAGCTGTATTCTCATCA+AGG 0.510551 5:-338948 MsG0580024057.01.T01:intron
CTGGAGTTATCTTCTTGTCA+TGG 0.515465 5:-338538 MsG0580024057.01.T01:CDS
ATAGGCAATGGTGAGCTCAT+AGG 0.515963 5:+338131 None:intergenic
GGCTAGTACTCTTGCAGGGG+AGG 0.516455 5:-339250 MsG0580024057.01.T01:CDS
CCCTAAGAGAGCAGGAATAC+GGG 0.517547 5:+339491 None:intergenic
AGAACAAAGATAACAAGAAA+GGG 0.517753 5:+339381 None:intergenic
TTTCTGCTTCAAACAATCCA+AGG 0.520360 5:+339019 None:intergenic
AGCAGGAGGCAAACAAAGCA+AGG 0.522399 5:+338370 None:intergenic
ACTTCTTGGAATGAGCAAGA+TGG 0.526526 5:-338977 MsG0580024057.01.T01:CDS
CCTCCAACTGAATGGGCTGA+TGG 0.532272 5:-339120 MsG0580024057.01.T01:CDS
CTATAGCATATAAGAAATTG+AGG 0.538180 5:+338489 None:intergenic
AACGTGATCCCTAAGAGAGC+AGG 0.540767 5:+339483 None:intergenic
CAGGAATACGGGCTAGCATA+CGG 0.541359 5:+339502 None:intergenic
CTAATCTTCTACGAAGTCTC+CGG 0.544287 5:-339816 MsG0580024057.01.T01:CDS
CCATCAGCCCATTCAGTTGG+AGG 0.547465 5:+339120 None:intergenic
GAGAATAAAATGCTAATAGT+CGG 0.552107 5:+338566 None:intergenic
CAAGTCGGGATGTTGATTGG+TGG 0.560117 5:-339087 MsG0580024057.01.T01:CDS
TCTTGGCTTGAACCAAGATA+GGG 0.561433 5:+338261 None:intergenic
CTCAACTCCAAATGGACCGC+CGG 0.561696 5:+339797 None:intergenic
TAACCATCAGCCCATTCAGT+TGG 0.562028 5:+339117 None:intergenic
CATCAGCCCATTCAGTTGGA+GGG 0.563433 5:+339121 None:intergenic
GAACATAGCCACCGTTGTGA+GGG 0.565973 5:+339661 None:intergenic
GACAATTCTTCTTCCACCAT+AGG 0.566948 5:+338113 None:intergenic
GTTTGGAACGGAACTCTGAA+AGG 0.570334 5:+338404 None:intergenic
TCAATTGATTCAGAACTGGT+TGG 0.570609 5:-338179 MsG0580024057.01.T01:CDS
ACTAAGGCGATGGTTTCTTG+TGG 0.570613 5:-339340 MsG0580024057.01.T01:CDS
TGTCATCACCTTCCAATGGT+TGG 0.570626 5:-338205 MsG0580024057.01.T01:CDS
GTGGTTCTAATCCAAAGTAG+AGG 0.573793 5:-338079 None:intergenic
AGTGGAGCAGTAGTAATCGT+TGG 0.575855 5:-338296 MsG0580024057.01.T01:CDS
CCTTCTTGAAAACCCCAAAA+GGG 0.576626 5:+339615 None:intergenic
ATCAGCCCATTCAGTTGGAG+GGG 0.581194 5:+339122 None:intergenic
TCTTCTTCCACCATAGGCAA+TGG 0.581759 5:+338119 None:intergenic
TCCGTTCCAAACATTTGGTG+CGG 0.582303 5:-338393 MsG0580024057.01.T01:CDS
ATAAGGCTAGTACTCTTGCA+GGG 0.582318 5:-339254 MsG0580024057.01.T01:CDS
CAGCGGTGATGAGAGCTTCA+GGG 0.582358 5:+339700 None:intergenic
ACAAGAATAAGTAACAAAGC+AGG 0.582392 5:+338353 None:intergenic
CCAAAATCAGACTAAGGCGA+TGG 0.584574 5:-339350 MsG0580024057.01.T01:CDS
AAGGTTAGACATGGCAGCGG+CGG 0.595108 5:+339038 None:intergenic
AGAACATAGCCACCGTTGTG+AGG 0.595254 5:+339660 None:intergenic
ACTGGTTGGCCATGAAAACA+AGG 0.596054 5:-338165 MsG0580024057.01.T01:CDS
TTTAATAACGTCAATTGGCG+TGG 0.601030 5:-339315 MsG0580024057.01.T01:CDS
AAATTGGTTACTGTTTGAAG+CGG 0.601450 5:-338234 MsG0580024057.01.T01:CDS
GAATTGTCTTTAATGCAAAG+TGG 0.601773 5:-338098 MsG0580024057.01.T01:CDS
TCTGTAATTCAAATAAGTAA+GGG 0.601990 5:+339458 None:intergenic
ATTGAATGCCAACCATTGGA+AGG 0.602235 5:+338197 None:intergenic
CTTACTTATTTGAATTACAG+AGG 0.605159 5:-339456 MsG0580024057.01.T01:CDS
GAGCTCACCATTGCCTATGG+TGG 0.608182 5:-338126 MsG0580024057.01.T01:CDS
GATTCTGTTAGAACCGGCGG+TGG 0.609699 5:-339774 MsG0580024057.01.T01:CDS
GCCGCACCAAATGTTTGGAA+CGG 0.620389 5:+338392 None:intergenic
GCACCTGTTCCTGCTAGCAA+CGG 0.624624 5:+339153 None:intergenic
TCAGGGACACTCCAAATGAG+AGG 0.625273 5:+339717 None:intergenic
AAGCCGAGTAAGGATAGAAG+AGG 0.626094 5:+339750 None:intergenic
TCCAAGGTTAGACATGGCAG+CGG 0.631162 5:+339035 None:intergenic
CAGGGACACTCCAAATGAGA+GGG 0.634528 5:+339718 None:intergenic
CAACTGTCATCACCTTCCAA+TGG 0.639749 5:-338209 MsG0580024057.01.T01:CDS
GGATAGAAGAGGACCACCGC+CGG 0.641963 5:+339761 None:intergenic
GAGGATTCTGTTAGAACCGG+CGG 0.644182 5:-339777 MsG0580024057.01.T01:CDS
TAGAAGATTAGAGCAATCAG+AGG 0.645302 5:+339828 None:intergenic
ATCTTCTACGAAGTCTCCGG+CGG 0.650130 5:-339813 MsG0580024057.01.T01:CDS
AGAATAAGTAACAAAGCAGG+AGG 0.650240 5:+338356 None:intergenic
GAAAGTAGTGGCGAGTTCAG+CGG 0.650242 5:+339683 None:intergenic
AAACAATCCAAGGTTAGACA+TGG 0.651742 5:+339029 None:intergenic
CCAAAAGGGACCAAAAGCAG+AGG 0.661685 5:+339629 None:intergenic
GCCACTACTTTCCCTCACAA+CGG 0.683972 5:-339672 MsG0580024057.01.T01:CDS
ACCGTTGTGAGGGAAAGTAG+TGG 0.687593 5:+339671 None:intergenic
TAAGGCTAGTACTCTTGCAG+GGG 0.692679 5:-339253 MsG0580024057.01.T01:CDS
ACTACTTTCCCTCACAACGG+TGG 0.702831 5:-339669 MsG0580024057.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGATTGTATATAAGCAATA+TGG - Chr5:339169-339188 MsG0580024057.01.T01:CDS 20.0%
!! TATTAATTGCTTGAACAAAA+AGG - Chr5:339314-339333 MsG0580024057.01.T01:CDS 20.0%
!! TCTGTAATTCAAATAAGTAA+GGG + Chr5:338506-338525 None:intergenic 20.0%
!!! GTTTTGGAATTTGAATTATT+GGG - Chr5:338684-338703 MsG0580024057.01.T01:intron 20.0%
!!! TATCTGTTTTGAAAAATAGA+TGG - Chr5:339193-339212 MsG0580024057.01.T01:CDS 20.0%
!!! TGTTTTGGAATTTGAATTAT+TGG - Chr5:338683-338702 MsG0580024057.01.T01:intron 20.0%
! AAAGGGTAAAAGTGAAAAAA+TGG + Chr5:338566-338585 None:intergenic 25.0%
! AAGAACAAAGATAACAAGAA+AGG + Chr5:338584-338603 None:intergenic 25.0%
! AAGTAATCAAGAAACAAAAC+AGG + Chr5:338415-338434 None:intergenic 25.0%
! AGAACAAAGATAACAAGAAA+GGG + Chr5:338583-338602 None:intergenic 25.0%
! ATTTGAACTTCATCCTTAAA+AGG - Chr5:339219-339238 MsG0580024057.01.T01:CDS 25.0%
! CTATAGCATATAAGAAATTG+AGG + Chr5:339475-339494 None:intergenic 25.0%
! CTCAATTTCTTATATGCTAT+AGG - Chr5:339473-339492 MsG0580024057.01.T01:CDS 25.0%
! CTCTGTAATTCAAATAAGTA+AGG + Chr5:338507-338526 None:intergenic 25.0%
! CTTACTTATTTGAATTACAG+AGG - Chr5:338505-338524 MsG0580024057.01.T01:CDS 25.0%
! GAATTTGAATTATTGGGATA+AGG - Chr5:338690-338709 MsG0580024057.01.T01:intron 25.0%
! GAGAATAAAATGCTAATAGT+CGG + Chr5:339398-339417 None:intergenic 25.0%
! TAAAAGAAAGATACCATACA+AGG + Chr5:339147-339166 None:intergenic 25.0%
! TTCAAGCCAAGAATAAAAAT+TGG - Chr5:339711-339730 MsG0580024057.01.T01:CDS 25.0%
! TTTGAACTTCATCCTTAAAA+GGG - Chr5:339220-339239 MsG0580024057.01.T01:CDS 25.0%
!! CAGTAACCAATTTTTATTCT+TGG + Chr5:339720-339739 None:intergenic 25.0%
!! GTTACTTCAATAACATGTTT+TGG - Chr5:338668-338687 MsG0580024057.01.T01:intron 25.0%
!!! ATTTAGTTTCATTTTCTACC+TGG + Chr5:339095-339114 None:intergenic 25.0%
!!! TGGATTTTAATAACGTCAAT+TGG - Chr5:338641-338660 MsG0580024057.01.T01:intron 25.0%
!!! TTTAGTTTCATTTTCTACCT+GGG + Chr5:339094-339113 None:intergenic 25.0%
AAACTTTCTAGGTCTAAGTA+TGG - Chr5:339368-339387 MsG0580024057.01.T01:CDS 30.0%
AAATTGGTTACTGTTTGAAG+CGG - Chr5:339727-339746 MsG0580024057.01.T01:CDS 30.0%
ACAAGAATAAGTAACAAAGC+AGG + Chr5:339611-339630 None:intergenic 30.0%
ATTTATCTTGTACCCTATCT+TGG - Chr5:339688-339707 MsG0580024057.01.T01:CDS 30.0%
CATACGGTTAAAAATAGGAA+AGG + Chr5:338446-338465 None:intergenic 30.0%
CTGAAAGGTCTATAAAGATT+TGG + Chr5:339545-339564 None:intergenic 30.0%
GAATTGTCTTTAATGCAAAG+TGG - Chr5:339863-339882 MsG0580024057.01.T01:CDS 30.0%
TGTGGTACTATAAACTTTCT+AGG - Chr5:339357-339376 MsG0580024057.01.T01:CDS 30.0%
TTGAACTTCATCCTTAAAAG+GGG - Chr5:339221-339240 MsG0580024057.01.T01:CDS 30.0%
! ACTTATTCTTGTTATGTGTC+TGG - Chr5:339619-339638 MsG0580024057.01.T01:CDS 30.0%
!! TTCAAGAAGGTTTTTGGAAA+TGG - Chr5:338359-338378 MsG0580024057.01.T01:CDS 30.0%
!!! AGAGGTAATAAGGTTAGTTT+TGG + Chr5:338118-338137 None:intergenic 30.0%
AAACAATCCAAGGTTAGACA+TGG + Chr5:338935-338954 None:intergenic 35.0%
AAAGATACCATACAAGGAGA+GGG + Chr5:339141-339160 None:intergenic 35.0%
ACAGTGATCTAACATTGATG+TGG - Chr5:339339-339358 MsG0580024057.01.T01:CDS 35.0%
ACCACTCACTTAAGACTAAA+TGG + Chr5:339270-339289 None:intergenic 35.0%
AGAATAAGTAACAAAGCAGG+AGG + Chr5:339608-339627 None:intergenic 35.0%
ATCAATTGAATGCCAACCAT+TGG + Chr5:339771-339790 None:intergenic 35.0%
CTCACTTAAGACTAAATGGT+AGG + Chr5:339266-339285 None:intergenic 35.0%
GCTAGCATACGGTTAAAAAT+AGG + Chr5:338451-338470 None:intergenic 35.0%
TAGAAGATTAGAGCAATCAG+AGG + Chr5:338136-338155 None:intergenic 35.0%
TAGAGCAATCAGAGGTAATA+AGG + Chr5:338128-338147 None:intergenic 35.0%
TTGAGAACGTTCTTTGTAAG+TGG - Chr5:339647-339666 MsG0580024057.01.T01:CDS 35.0%
TTTAATAACGTCAATTGGCG+TGG - Chr5:338646-338665 MsG0580024057.01.T01:intron 35.0%
TTTCTGCTTCAAACAATCCA+AGG + Chr5:338945-338964 None:intergenic 35.0%
! AGCATTTTATTCTCTGCAAC+TGG - Chr5:339404-339423 MsG0580024057.01.T01:CDS 35.0%
! AGTGATGCTTTTCAACTTCT+TGG - Chr5:338970-338989 MsG0580024057.01.T01:CDS 35.0%
! GAAATGGTTTAGTGGTGTTA+TGG - Chr5:338375-338394 MsG0580024057.01.T01:CDS 35.0%
! GCATTCAATTGATTCAGAAC+TGG - Chr5:339778-339797 MsG0580024057.01.T01:CDS 35.0%
! TCAATTGATTCAGAACTGGT+TGG - Chr5:339782-339801 MsG0580024057.01.T01:CDS 35.0%
! TCTTCTTTTTCCCTCTCATT+TGG - Chr5:338233-338252 MsG0580024057.01.T01:CDS 35.0%
!! ACCATTTAGTCTTAAGTGAG+TGG - Chr5:339266-339285 MsG0580024057.01.T01:CDS 35.0%
!! ATGGTTATTTTGCTCAAGTC+GGG - Chr5:338860-338879 MsG0580024057.01.T01:intron 35.0%
!! GATGGTTATTTTGCTCAAGT+CGG - Chr5:338859-338878 MsG0580024057.01.T01:intron 35.0%
!! GGTTTTTGGAAATGGTTTAG+TGG - Chr5:338367-338386 MsG0580024057.01.T01:CDS 35.0%
!!! CTTTCCCAAAGCACTTTTTT+GGG - Chr5:338753-338772 MsG0580024057.01.T01:intron 35.0%
!!! TTTCCCAAAGCACTTTTTTG+GGG - Chr5:338754-338773 MsG0580024057.01.T01:intron 35.0%
ACAGAATCCTCAACTCCAAA+TGG + Chr5:338175-338194 None:intergenic 40.0%
ACCTTCTTGAAAACCCCAAA+AGG + Chr5:338350-338369 None:intergenic 40.0%
ACTTCTTGGAATGAGCAAGA+TGG - Chr5:338984-339003 MsG0580024057.01.T01:CDS 40.0%
AGAGTTCCGTTCCAAACATT+TGG - Chr5:339563-339582 MsG0580024057.01.T01:CDS 40.0%
ATAAGGCTAGTACTCTTGCA+GGG - Chr5:338707-338726 MsG0580024057.01.T01:intron 40.0%
ATTGAATGCCAACCATTGGA+AGG + Chr5:339767-339786 None:intergenic 40.0%
CCTTAAAAGGGGATTTGTAG+TGG - Chr5:339232-339251 MsG0580024057.01.T01:CDS 40.0%
CCTTCTTGAAAACCCCAAAA+GGG + Chr5:338349-338368 None:intergenic 40.0%
CTAATCTTCTACGAAGTCTC+CGG - Chr5:338145-338164 MsG0580024057.01.T01:CDS 40.0%
CTGGAGTTATCTTCTTGTCA+TGG - Chr5:339423-339442 MsG0580024057.01.T01:CDS 40.0%
CTGTTCCCAAAATCAGACTA+AGG - Chr5:338605-338624 MsG0580024057.01.T01:intron 40.0%
CTTCTTGGAATGAGCAAGAT+GGG - Chr5:338985-339004 MsG0580024057.01.T01:CDS 40.0%
GAAAAAGAAGAAGCCGAGTA+AGG + Chr5:338224-338243 None:intergenic 40.0%
GAAAGATACCATACAAGGAG+AGG + Chr5:339142-339161 None:intergenic 40.0%
GACAATTCTTCTTCCACCAT+AGG + Chr5:339851-339870 None:intergenic 40.0%
GATGAGCTTTCAGCAAATAC+TGG - Chr5:339445-339464 MsG0580024057.01.T01:CDS 40.0%
GGGAAAGAGAAAGAGAAAGA+GGG + Chr5:339074-339093 None:intergenic 40.0%
GTTGAGGATTCTGTTAGAAC+CGG - Chr5:338181-338200 MsG0580024057.01.T01:CDS 40.0%
TACAGAGGTCTTCACATTGT+TGG - Chr5:338520-338539 MsG0580024057.01.T01:CDS 40.0%
TATTTGATTCCGTTGCTAGC+AGG - Chr5:338799-338818 MsG0580024057.01.T01:intron 40.0%
TCTTTCTCTTTCTCTTTCCC+AGG - Chr5:339074-339093 MsG0580024057.01.T01:CDS 40.0%
TGGGAAAGAGAAAGAGAAAG+AGG + Chr5:339075-339094 None:intergenic 40.0%
TTCCAGCTGTATTCTCATCA+AGG - Chr5:339013-339032 MsG0580024057.01.T01:CDS 40.0%
! CATCGCCTTAGTCTGATTTT+GGG + Chr5:338613-338632 None:intergenic 40.0%
! CCACTACAAATCCCCTTTTA+AGG + Chr5:339235-339254 None:intergenic 40.0%
! CTTTGGATATCCTCTGCTTT+TGG - Chr5:338322-338341 MsG0580024057.01.T01:CDS 40.0%
! TCTTGGCTTGAACCAAGATA+GGG + Chr5:339703-339722 None:intergenic 40.0%
! TTCTTGGCTTGAACCAAGAT+AGG + Chr5:339704-339723 None:intergenic 40.0%
!! ATCCCCCAAAAAAGTGCTTT+GGG + Chr5:338760-338779 None:intergenic 40.0%
!! CCAAAAAAGTGCTTTGGGAA+AGG + Chr5:338755-338774 None:intergenic 40.0%
!! GGATGTTGATTGGTGGTTTT+TGG - Chr5:338881-338900 MsG0580024057.01.T01:intron 40.0%
!! GGGGTTTTCAAGAAGGTTTT+TGG - Chr5:338353-338372 MsG0580024057.01.T01:CDS 40.0%
!!! ACTTTTTTGGGGGATGCTTT+TGG - Chr5:338765-338784 MsG0580024057.01.T01:intron 40.0%
!!! CCTTTCCCAAAGCACTTTTT+TGG - Chr5:338752-338771 MsG0580024057.01.T01:intron 40.0%
!!! GCTTTGGGAAAGGTTTTACT+TGG + Chr5:338745-338764 None:intergenic 40.0%
!!! GTGGTTTTTGGCTTAAACTG+TGG - Chr5:338893-338912 MsG0580024057.01.T01:intron 40.0%
!!! TTCCCAAAGCACTTTTTTGG+GGG - Chr5:338755-338774 MsG0580024057.01.T01:intron 40.0%
AAGCCGAGTAAGGATAGAAG+AGG + Chr5:338214-338233 None:intergenic 45.0%
ACAACGGTGGCTATGTTCTT+TGG - Chr5:338305-338324 MsG0580024057.01.T01:CDS 45.0%
ACTAAGGCGATGGTTTCTTG+TGG - Chr5:338621-338640 MsG0580024057.01.T01:intron 45.0%
AGTGGAGCAGTAGTAATCGT+TGG - Chr5:339665-339684 MsG0580024057.01.T01:CDS 45.0%
ATAGGCAATGGTGAGCTCAT+AGG + Chr5:339833-339852 None:intergenic 45.0%
CAACTGTCATCACCTTCCAA+TGG - Chr5:339752-339771 MsG0580024057.01.T01:CDS 45.0%
CCAAAATCAGACTAAGGCGA+TGG - Chr5:338611-338630 MsG0580024057.01.T01:intron 45.0%
GACCTTGATGAGAATACAGC+TGG + Chr5:339018-339037 None:intergenic 45.0%
GATAAGGCTAGTACTCTTGC+AGG - Chr5:338706-338725 MsG0580024057.01.T01:intron 45.0%
GGTCCTCTTCTATCCTTACT+CGG - Chr5:338208-338227 MsG0580024057.01.T01:CDS 45.0%
TAACCATCAGCCCATTCAGT+TGG + Chr5:338847-338866 None:intergenic 45.0%
TAAGGCTAGTACTCTTGCAG+GGG - Chr5:338708-338727 MsG0580024057.01.T01:intron 45.0%
TATGAGCTCACCATTGCCTA+TGG - Chr5:339832-339851 MsG0580024057.01.T01:CDS 45.0%
TCCCTAAGAGAGCAGGAATA+CGG + Chr5:338474-338493 None:intergenic 45.0%
TCTTCTTCCACCATAGGCAA+TGG + Chr5:339845-339864 None:intergenic 45.0%
TGTCATCACCTTCCAATGGT+TGG - Chr5:339756-339775 MsG0580024057.01.T01:CDS 45.0%
TGTGATACCCTCTCCTTGTA+TGG - Chr5:339131-339150 MsG0580024057.01.T01:CDS 45.0%
! ACTGGTTGGCCATGAAAACA+AGG - Chr5:339796-339815 MsG0580024057.01.T01:CDS 45.0%
! CCATCGCCTTAGTCTGATTT+TGG + Chr5:338614-338633 None:intergenic 45.0%
! GTTTGGAACGGAACTCTGAA+AGG + Chr5:339560-339579 None:intergenic 45.0%
! TCCGTTCCAAACATTTGGTG+CGG - Chr5:339568-339587 MsG0580024057.01.T01:CDS 45.0%
!! CATCCCCCAAAAAAGTGCTT+TGG + Chr5:338761-338780 None:intergenic 45.0%
!! CTCTGCTTTTGGTCCCTTTT+GGG - Chr5:338333-338352 MsG0580024057.01.T01:CDS 45.0%
!! TCTGCTTTTGGTCCCTTTTG+GGG - Chr5:338334-338353 MsG0580024057.01.T01:CDS 45.0%
!! TTTTTGGGGGATGCTTTTGG+TGG - Chr5:338768-338787 MsG0580024057.01.T01:intron 45.0%
!!! CCCTTTTGGGGTTTTCAAGA+AGG - Chr5:338346-338365 MsG0580024057.01.T01:CDS 45.0%
!!! GCTCTGTGTCCTTGTTTTCA+TGG + Chr5:339808-339827 None:intergenic 45.0%
AACGTGATCCCTAAGAGAGC+AGG + Chr5:338481-338500 None:intergenic 50.0%
ACCGTTGTGAGGGAAAGTAG+TGG + Chr5:338293-338312 None:intergenic 50.0%
ACTACTTTCCCTCACAACGG+TGG - Chr5:338292-338311 MsG0580024057.01.T01:CDS 50.0%
AGAACATAGCCACCGTTGTG+AGG + Chr5:338304-338323 None:intergenic 50.0%
AGCAGGAGGCAAACAAAGCA+AGG + Chr5:339594-339613 None:intergenic 50.0%
ATCAGCCCATTCAGTTGGAG+GGG + Chr5:338842-338861 None:intergenic 50.0%
ATCTTCTACGAAGTCTCCGG+CGG - Chr5:338148-338167 MsG0580024057.01.T01:CDS 50.0%
ATTCCGTTGCTAGCAGGAAC+AGG - Chr5:338805-338824 MsG0580024057.01.T01:intron 50.0%
CAGGGACACTCCAAATGAGA+GGG + Chr5:338246-338265 None:intergenic 50.0%
CATCAGCCCATTCAGTTGGA+GGG + Chr5:338843-338862 None:intergenic 50.0%
CCAAAAGGGACCAAAAGCAG+AGG + Chr5:338335-338354 None:intergenic 50.0%
CCCGTATTCCTGCTCTCTTA+GGG - Chr5:338470-338489 MsG0580024057.01.T01:CDS 50.0%
CCCTAAGAGAGCAGGAATAC+GGG + Chr5:338473-338492 None:intergenic 50.0%
GAAAGTAGTGGCGAGTTCAG+CGG + Chr5:338281-338300 None:intergenic 50.0%
GAACATAGCCACCGTTGTGA+GGG + Chr5:338303-338322 None:intergenic 50.0%
GAGGATTCTGTTAGAACCGG+CGG - Chr5:338184-338203 MsG0580024057.01.T01:CDS 50.0%
GAGTTCCCCTCCAACTGAAT+GGG - Chr5:338834-338853 MsG0580024057.01.T01:intron 50.0%
GCCACTACTTTCCCTCACAA+CGG - Chr5:338289-338308 MsG0580024057.01.T01:CDS 50.0%
GCCGCACCAAATGTTTGGAA+CGG + Chr5:339572-339591 None:intergenic 50.0%
GCTCAAGTCGGGATGTTGAT+TGG - Chr5:338871-338890 MsG0580024057.01.T01:intron 50.0%
TCAGGGACACTCCAAATGAG+AGG + Chr5:338247-338266 None:intergenic 50.0%
TCCAAGGTTAGACATGGCAG+CGG + Chr5:338929-338948 None:intergenic 50.0%
TGAGTTCCCCTCCAACTGAA+TGG - Chr5:338833-338852 MsG0580024057.01.T01:intron 50.0%
! CAAGTCGGGATGTTGATTGG+TGG - Chr5:338874-338893 MsG0580024057.01.T01:intron 50.0%
!! CAGGAATACGGGCTAGCATA+CGG + Chr5:338462-338481 None:intergenic 50.0%
!! CCTCTGCTTTTGGTCCCTTT+TGG - Chr5:338332-338351 MsG0580024057.01.T01:CDS 50.0%
AAGGTTAGACATGGCAGCGG+CGG + Chr5:338926-338945 None:intergenic 55.0%
CAGCGGTGATGAGAGCTTCA+GGG + Chr5:338264-338283 None:intergenic 55.0%
CCATCAGCCCATTCAGTTGG+AGG + Chr5:338844-338863 None:intergenic 55.0%
CTCAACTCCAAATGGACCGC+CGG + Chr5:338167-338186 None:intergenic 55.0%
GAGCTCACCATTGCCTATGG+TGG - Chr5:339835-339854 MsG0580024057.01.T01:CDS 55.0%
GATTCTGTTAGAACCGGCGG+TGG - Chr5:338187-338206 MsG0580024057.01.T01:CDS 55.0%
GCAAGGCCGCACCAAATGTT+TGG + Chr5:339577-339596 None:intergenic 55.0%
GCACCTGTTCCTGCTAGCAA+CGG + Chr5:338811-338830 None:intergenic 55.0%
GCCCGTATTCCTGCTCTCTT+AGG - Chr5:338469-338488 MsG0580024057.01.T01:CDS 55.0%
TCAGCGGTGATGAGAGCTTC+AGG + Chr5:338265-338284 None:intergenic 55.0%
!! CCTCCAACTGAATGGGCTGA+TGG - Chr5:338841-338860 MsG0580024057.01.T01:intron 55.0%
GAAGTCTCCGGCGGTCCATT+TGG - Chr5:338157-338176 MsG0580024057.01.T01:CDS 60.0%
GCCGCTGCCATGTCTAACCT+TGG - Chr5:338925-338944 MsG0580024057.01.T01:intron 60.0%
GGATAGAAGAGGACCACCGC+CGG + Chr5:338203-338222 None:intergenic 60.0%
GGCTAGTACTCTTGCAGGGG+AGG - Chr5:338711-338730 MsG0580024057.01.T01:intron 60.0%
! CGGCGGTCCATTTGGAGTTG+AGG - Chr5:338165-338184 MsG0580024057.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 338082 339901 338082 ID=MsG0580024057.01;Name=MsG0580024057.01
Chr5 mRNA 338082 339901 338082 ID=MsG0580024057.01.T01;Parent=MsG0580024057.01;Name=MsG0580024057.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|1|1|2|0|491
Chr5 exon 338949 339901 338949 ID=MsG0580024057.01.T01:exon:77;Parent=MsG0580024057.01.T01
Chr5 exon 338082 338604 338082 ID=MsG0580024057.01.T01:exon:76;Parent=MsG0580024057.01.T01
Chr5 CDS 338949 339901 338949 ID=MsG0580024057.01.T01:cds;Parent=MsG0580024057.01.T01
Chr5 CDS 338082 338604 338082 ID=MsG0580024057.01.T01:cds;Parent=MsG0580024057.01.T01
Gene Sequence

>MsG0580024057.01.T01

ATGGATGATGATGAAGTAGTAACTGCTGCTGCTCCAAAACTAACCTTATTACCTCTGATTGCTCTAATCTTCTACGAAGTCTCCGGCGGTCCATTTGGAGTTGAGGATTCTGTTAGAACCGGCGGTGGTCCTCTTCTATCCTTACTCGGCTTCTTCTTTTTCCCTCTCATTTGGAGTGTCCCTGAAGCTCTCATCACCGCTGAACTCGCCACTACTTTCCCTCACAACGGTGGCTATGTTCTTTGGATATCCTCTGCTTTTGGTCCCTTTTGGGGTTTTCAAGAAGGTTTTTGGAAATGGTTTAGTGGTGTTATGGACAATGCTCTTTATCCTGTTTTGTTTCTTGATTACTTGAAACATTCCTTTCCTATTTTTAACCGTATGCTAGCCCGTATTCCTGCTCTCTTAGGGATCACGTTTTCCCTTACTTATTTGAATTACAGAGGTCTTCACATTGTTGGTTTCTCTGCTCTTGTTTTAACCATTTTTTCACTTTTACCCTTTCTTGTTATCTTTGTTCTTTCTGTTCCCAAAATCAGACTAAGGCGATGGTTTCTTGTGGATTTTAATAACGTCAATTGGCGTGGTTACTTCAATAACATGTTTTGGAATTTGAATTATTGGGATAAGGCTAGTACTCTTGCAGGGGAGGTTGAGAATCCAAGTAAAACCTTTCCCAAAGCACTTTTTTGGGGGATGCTTTTGGTGGTCTCTTCGTATTTGATTCCGTTGCTAGCAGGAACAGGTGCTTTGAGTTCCCCTCCAACTGAATGGGCTGATGGTTATTTTGCTCAAGTCGGGATGTTGATTGGTGGTTTTTGGCTTAAACTGTGGATTCAAGCCGCCGCTGCCATGTCTAACCTTGGATTGTTTGAAGCAGAAATGAGTAGTGATGCTTTTCAACTTCTTGGAATGAGCAAGATGGGATTGCTTCCAGCTGTATTCTCATCAAGGTCTAAGTATGGAACGCCGACTATTAGCATTTTATTCTCTGCAACTGGAGTTATCTTCTTGTCATGGATGAGCTTTCAGCAAATACTGGAGTTCCTCAATTTCTTATATGCTATAGGAATGCTTCTTGAGTTTGCAGCTTTTATAACTTTGAGATTGAAGAAGCCAAATCTTTATAGACCTTTCAGAGTTCCGTTCCAAACATTTGGTGCGGCCTTGCTTTGTTTGCCTCCTGCTTTGTTACTTATTCTTGTTATGTGTCTGGCTTCTTTGAGAACGTTCTTTGTAAGTGGAGCAGTAGTAATCGTTGGATTTATCTTGTACCCTATCTTGGTTCAAGCCAAGAATAAAAATTGGTTACTGTTTGAAGCGGAACAACTGTCATCACCTTCCAATGGTTGGCATTCAATTGATTCAGAACTGGTTGGCCATGAAAACAAGGACACAGAGCTTCCTATGAGCTCACCATTGCCTATGGTGGAAGAAGAATTGTCTTTAATGCAAAGTGGTTCTAATCCAAAGTAG
Protein sequence

>MsG0580024057.01.T01

MDDDEVVTAAAPKLTLLPLIALIFYEVSGGPFGVEDSVRTGGGPLLSLLGFFFFPLIWSVPEALITAELATTFPHNGGYVLWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKHSFPIFNRMLARIPALLGITFSLTYLNYRGLHIVGFSALVLTIFSLLPFLVIFVLSVPKIRLRRWFLVDFNNVNWRGYFNNMFWNLNYWDKASTLAGEVENPSKTFPKALFWGMLLVVSSYLIPLLAGTGALSSPPTEWADGYFAQVGMLIGGFWLKLWIQAAAAMSNLGLFEAEMSSDAFQLLGMSKMGLLPAVFSSRSKYGTPTISILFSATGVIFLSWMSFQQILEFLNFLYAIGMLLEFAAFITLRLKKPNLYRPFRVPFQTFGAALLCLPPALLLILVMCLASLRTFFVSGAVVIVGFILYPILVQAKNKNWLLFEAEQLSSPSNGWHSIDSELVGHENKDTELPMSSPLPMVEEELSLMQSGSNPK*