Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024132.01.T01 | AES93671.1 | 98.333 | 60 | 1 | 0 | 1 | 60 | 1 | 60 | 6.92E-35 | 122 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024132.01.T01 | G7K2D7 | 98.333 | 60 | 1 | 0 | 1 | 60 | 1 | 60 | 3.30e-35 | 122 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580024132.01 | MsG0880047654.01 | 0.800335 | 1.512679e-48 | 7.025187e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024132.01.T01 | MTR_5g006200 | 98.333 | 60 | 1 | 0 | 1 | 60 | 1 | 60 | 8.38e-39 | 122 |
| MsG0580024132.01.T01 | MTR_1g104520 | 89.831 | 59 | 6 | 0 | 1 | 59 | 1 | 59 | 5.24e-35 | 112 |
| MsG0580024132.01.T01 | MTR_7g095120 | 73.770 | 61 | 15 | 1 | 1 | 60 | 1 | 61 | 6.16e-28 | 95.1 |
| MsG0580024132.01.T01 | MTR_5g006220 | 72.414 | 58 | 16 | 0 | 1 | 58 | 1 | 58 | 7.57e-24 | 84.7 |
| MsG0580024132.01.T01 | MTR_5g006260 | 46.914 | 81 | 19 | 1 | 1 | 57 | 1 | 81 | 2.38e-18 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTCCATTGAAGAAGGTT+TGG | 0.332984 | 5:+1225725 | MsG0580024132.01.T01:CDS |
| GAGGACTTGCACATCTTCAT+AGG | 0.366200 | 5:-1226109 | None:intergenic |
| TTGAACCAAACCTTCTTCAA+TGG | 0.373631 | 5:-1225730 | None:intergenic |
| CCTCTTCTCTCCATTGAAGA+AGG | 0.408548 | 5:+1225720 | MsG0580024132.01.T01:CDS |
| ATGAAGATGTGCAAGTCCTC+TGG | 0.501528 | 5:+1226112 | MsG0580024132.01.T01:CDS |
| CTTCTTCAATGGAGAGAAGA+GGG | 0.524453 | 5:-1225719 | None:intergenic |
| AGGACTTGCACATCTTCATA+GGG | 0.526752 | 5:-1226108 | None:intergenic |
| AGTCCTCTGGTCGATACTAG+TGG | 0.584601 | 5:+1226125 | MsG0580024132.01.T01:CDS |
| CCTTCTTCAATGGAGAGAAG+AGG | 0.605757 | 5:-1225720 | None:intergenic |
| ACTCATTTCATCAAATGGGG+TGG | 0.632560 | 5:+1225689 | None:intergenic |
| GGACTTGCACATCTTCATAG+GGG | 0.720103 | 5:-1226107 | None:intergenic |
| ATTCCACTAGTATCGACCAG+AGG | 0.745202 | 5:-1226128 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAAAAAAAAAAATTTAT+TGG | - | Chr5:1226038-1226057 | None:intergenic | 0.0% |
| !!! | TTTTTTTTTAAAATTTTTGC+AGG | + | Chr5:1226045-1226064 | MsG0580024132.01.T01:intron | 10.0% |
| !! | TTACAACTTTACAAGAAATA+AGG | - | Chr5:1225822-1225841 | None:intergenic | 20.0% |
| ! | ATGAAATTTCATTGAAGTGA+AGG | - | Chr5:1225969-1225988 | None:intergenic | 25.0% |
| ! | TACTAAGAACAATGAAAAAG+AGG | - | Chr5:1225876-1225895 | None:intergenic | 25.0% |
| !! | TTAAAATTTTTGCAGGAAGA+GGG | + | Chr5:1226052-1226071 | MsG0580024132.01.T01:intron | 25.0% |
| !!! | TTGATTGTTTTTTTCTTCCA+AGG | - | Chr5:1225919-1225938 | None:intergenic | 25.0% |
| !!! | TTTAAAATTTTTGCAGGAAG+AGG | + | Chr5:1226051-1226070 | MsG0580024132.01.T01:intron | 25.0% |
| AAGGAAAAACAGACAATGCA+GGG | - | Chr5:1225803-1225822 | None:intergenic | 35.0% | |
| TAAGGAAAAACAGACAATGC+AGG | - | Chr5:1225804-1225823 | None:intergenic | 35.0% | |
| TGTACAAAGCTTCTTATCCT+TGG | + | Chr5:1225899-1225918 | MsG0580024132.01.T01:intron | 35.0% | |
| TTGAACCAAACCTTCTTCAA+TGG | - | Chr5:1225733-1225752 | None:intergenic | 35.0% | |
| TTTCATTGAAGTGAAGGTGA+AGG | - | Chr5:1225963-1225982 | None:intergenic | 35.0% | |
| AGGACTTGCACATCTTCATA+GGG | - | Chr5:1226111-1226130 | None:intergenic | 40.0% | |
| ATTGAAGTGAAGGTGAAGGA+GGG | - | Chr5:1225959-1225978 | None:intergenic | 40.0% | |
| CTTCTTCAATGGAGAGAAGA+GGG | - | Chr5:1225722-1225741 | None:intergenic | 40.0% | |
| TCTCTCCATTGAAGAAGGTT+TGG | + | Chr5:1225725-1225744 | MsG0580024132.01.T01:CDS | 40.0% | |
| ATGAAGATGTGCAAGTCCTC+TGG | + | Chr5:1226112-1226131 | MsG0580024132.01.T01:CDS | 45.0% | |
| CATTGAAGTGAAGGTGAAGG+AGG | - | Chr5:1225960-1225979 | None:intergenic | 45.0% | |
| CCTCTTCTCTCCATTGAAGA+AGG | + | Chr5:1225720-1225739 | MsG0580024132.01.T01:CDS | 45.0% | |
| CCTTCTTCAATGGAGAGAAG+AGG | - | Chr5:1225723-1225742 | None:intergenic | 45.0% | |
| GAGGACTTGCACATCTTCAT+AGG | - | Chr5:1226112-1226131 | None:intergenic | 45.0% | |
| GGACTTGCACATCTTCATAG+GGG | - | Chr5:1226110-1226129 | None:intergenic | 45.0% | |
| !! | ATTCCACTAGTATCGACCAG+AGG | - | Chr5:1226131-1226150 | None:intergenic | 45.0% |
| !! | GCAGGGTATGAAAAGTGTCA+TGG | - | Chr5:1225786-1225805 | None:intergenic | 45.0% |
| AGTCCTCTGGTCGATACTAG+TGG | + | Chr5:1226125-1226144 | MsG0580024132.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 1225703 | 1226176 | 1225703 | ID=MsG0580024132.01;Name=MsG0580024132.01 |
| Chr5 | mRNA | 1225703 | 1226176 | 1225703 | ID=MsG0580024132.01.T01;Parent=MsG0580024132.01;Name=MsG0580024132.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0|0|1|0|0|2|0|60 |
| Chr5 | exon | 1225703 | 1225775 | 1225703 | ID=MsG0580024132.01.T01:exon:23387;Parent=MsG0580024132.01.T01 |
| Chr5 | exon | 1226067 | 1226176 | 1226067 | ID=MsG0580024132.01.T01:exon:23388;Parent=MsG0580024132.01.T01 |
| Chr5 | CDS | 1225703 | 1225775 | 1225703 | ID=MsG0580024132.01.T01:cds;Parent=MsG0580024132.01.T01 |
| Chr5 | CDS | 1226067 | 1226176 | 1226067 | ID=MsG0580024132.01.T01:cds;Parent=MsG0580024132.01.T01 |
| Gene Sequence |
| Protein sequence |
