Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047654.01.T01 | AES92639.2 | 94.286 | 70 | 3 | 1 | 1 | 69 | 40 | 109 | 5.45E-38 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047654.01.T01 | G7JJ82 | 94.286 | 70 | 3 | 1 | 1 | 69 | 40 | 109 | 2.60e-38 | 132 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000466.01 | MsG0880047654.01 | 0.809302 | 2.023457e-50 | 1.183752e-47 |
| MsG0880047090.01 | MsG0880047654.01 | 0.819340 | 1.222409e-52 | 9.380301e-50 |
| MsG0580024132.01 | MsG0880047654.01 | 0.800335 | 1.512679e-48 | 7.025187e-46 |
| MsG0780038919.01 | MsG0880047654.01 | 0.835510 | 1.609145e-56 | 1.971960e-53 |
| MsG0780039536.01 | MsG0880047654.01 | 0.805063 | 1.599696e-49 | 8.382128e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047654.01.T01 | MTR_4g132230 | 94.286 | 70 | 3 | 1 | 1 | 69 | 40 | 109 | 6.60e-42 | 132 |
| MsG0880047654.01.T01 | MTR_2g023440 | 49.351 | 77 | 24 | 3 | 1 | 62 | 1 | 77 | 3.40e-13 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047654.01.T01 | AT1G62045 | 49.206 | 63 | 29 | 2 | 1 | 62 | 1 | 61 | 4.95e-13 | 57.8 |
| MsG0880047654.01.T01 | AT1G11740 | 49.231 | 65 | 30 | 2 | 1 | 62 | 573 | 637 | 3.01e-11 | 57.0 |
Find 22 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGGGGTGCTGGTGGTTT+TGG | 0.215218 | 8:-89259962 | MsG0880047654.01.T01:CDS |
| CAACCAAGCAACTGGGTTAA+TGG | 0.264966 | 8:-89259802 | None:intergenic |
| ACAGTTTGAGGCAGTTGCTT+TGG | 0.357175 | 8:+89259862 | None:intergenic |
| AAAGACACCAACCAAGCAAC+TGG | 0.372401 | 8:-89259810 | MsG0880047654.01.T01:CDS |
| CATTAACCCAGTTGCTTGGT+TGG | 0.384881 | 8:+89259803 | None:intergenic |
| ACCAAGTTGGGCAGATCAAT+GGG | 0.402298 | 8:-89259978 | MsG0880047654.01.T01:CDS |
| GGCAACGGAGGCACCAAGTT+GGG | 0.426929 | 8:-89259990 | MsG0880047654.01.T01:CDS |
| CTCCCATTAACCCAGTTGCT+TGG | 0.427988 | 8:+89259799 | None:intergenic |
| CACCAAGTTGGGCAGATCAA+TGG | 0.454043 | 8:-89259979 | MsG0880047654.01.T01:CDS |
| TTGGTGTCTTTCTCTTGCAA+AGG | 0.463805 | 8:+89259822 | None:intergenic |
| TGGGCAGATCAATGGGGTGC+TGG | 0.472690 | 8:-89259971 | MsG0880047654.01.T01:CDS |
| CCCCATTGATCTGCCCAACT+TGG | 0.476423 | 8:+89259977 | None:intergenic |
| TAACCACTTTACACAGTTTG+AGG | 0.477436 | 8:+89259850 | None:intergenic |
| TTGGCTTTGCATAAAGCACC+TGG | 0.501985 | 8:+89259881 | None:intergenic |
| GCAGATCAATGGGGTGCTGG+TGG | 0.521164 | 8:-89259968 | MsG0880047654.01.T01:CDS |
| AGCAAGAAGAGCAAGAATCC+AGG | 0.523096 | 8:-89259899 | MsG0880047654.01.T01:CDS |
| CTGCCTCAAACTGTGTAAAG+TGG | 0.553790 | 8:-89259853 | MsG0880047654.01.T01:CDS |
| TGGCAACGGAGGCACCAAGT+TGG | 0.565641 | 8:-89259991 | MsG0880047654.01.T01:CDS |
| CCTAGGAAACATGGCAACGG+AGG | 0.612608 | 8:-89260002 | None:intergenic |
| GGCCCTAGGAAACATGGCAA+CGG | 0.626735 | 8:-89260005 | None:intergenic |
| AAGACACCAACCAAGCAACT+GGG | 0.633568 | 8:-89259809 | MsG0880047654.01.T01:CDS |
| CCAAGTTGGGCAGATCAATG+GGG | 0.727100 | 8:-89259977 | MsG0880047654.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAACCACTTTACACAGTTTG+AGG | + | Chr8:89259950-89259969 | None:intergenic | 35.0% | |
| ! | TTGGTGTCTTTCTCTTGCAA+AGG | + | Chr8:89259978-89259997 | None:intergenic | 40.0% |
| AAAGACACCAACCAAGCAAC+TGG | - | Chr8:89259987-89260006 | MsG0880047654.01.T01:CDS | 45.0% | |
| AAGACACCAACCAAGCAACT+GGG | - | Chr8:89259988-89260007 | MsG0880047654.01.T01:CDS | 45.0% | |
| AGCAAGAAGAGCAAGAATCC+AGG | - | Chr8:89259898-89259917 | MsG0880047654.01.T01:CDS | 45.0% | |
| CTGCCTCAAACTGTGTAAAG+TGG | - | Chr8:89259944-89259963 | MsG0880047654.01.T01:CDS | 45.0% | |
| ! | ACAGTTTGAGGCAGTTGCTT+TGG | + | Chr8:89259938-89259957 | None:intergenic | 45.0% |
| ! | ACCAAGTTGGGCAGATCAAT+GGG | - | Chr8:89259819-89259838 | MsG0880047654.01.T01:CDS | 45.0% |
| !! | TTGGCTTTGCATAAAGCACC+TGG | + | Chr8:89259919-89259938 | None:intergenic | 45.0% |
| ! | CACCAAGTTGGGCAGATCAA+TGG | - | Chr8:89259818-89259837 | MsG0880047654.01.T01:CDS | 50.0% |
| ! | CCAAGTTGGGCAGATCAATG+GGG | - | Chr8:89259820-89259839 | MsG0880047654.01.T01:CDS | 50.0% |
| !!! | TGCTGGTGGTTTTGGTGCAA+TGG | - | Chr8:89259843-89259862 | MsG0880047654.01.T01:CDS | 50.0% |
| CCCCATTGATCTGCCCAACT+TGG | + | Chr8:89259823-89259842 | None:intergenic | 55.0% | |
| !!! | CAATGGGGTGCTGGTGGTTT+TGG | - | Chr8:89259835-89259854 | MsG0880047654.01.T01:CDS | 55.0% |
| TGGGCAGATCAATGGGGTGC+TGG | - | Chr8:89259826-89259845 | MsG0880047654.01.T01:CDS | 60.0% | |
| !! | GCAGATCAATGGGGTGCTGG+TGG | - | Chr8:89259829-89259848 | MsG0880047654.01.T01:CDS | 60.0% |
| !! | GGCAACGGAGGCACCAAGTT+GGG | - | Chr8:89259807-89259826 | MsG0880047654.01.T01:CDS | 60.0% |
| !! | TGGCAACGGAGGCACCAAGT+TGG | - | Chr8:89259806-89259825 | MsG0880047654.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 89259805 | 89260014 | 89259805 | ID=MsG0880047654.01;Name=MsG0880047654.01 |
| Chr8 | mRNA | 89259805 | 89260014 | 89259805 | ID=MsG0880047654.01.T01;Parent=MsG0880047654.01;Name=MsG0880047654.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|69 |
| Chr8 | exon | 89259805 | 89260014 | 89259805 | ID=MsG0880047654.01.T01:exon:38819;Parent=MsG0880047654.01.T01 |
| Chr8 | CDS | 89259805 | 89260014 | 89259805 | ID=MsG0880047654.01.T01:cds;Parent=MsG0880047654.01.T01 |
| Gene Sequence |
| Protein sequence |
