AlfalfaGEDB | MsG0880047654.01

M. sativa cultivar ZhongmuNo.1 / MsG0880047654.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047654.01.T01	AES92639.2	94.286	70	3	1	1	69	40	109	5.45E-38	132

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047654.01.T01	G7JJ82	94.286	70	3	1	1	69	40	109	2.60e-38	132

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000466.01	MsG0880047654.01	0.809302	2.023457e-50	1.183752e-47
MsG0580024132.01	MsG0880047654.01	0.800335	1.512679e-48	7.025187e-46
MsG0780038919.01	MsG0880047654.01	0.835510	1.609145e-56	1.971960e-53
MsG0780039536.01	MsG0880047654.01	0.805063	1.599696e-49	8.382128e-47
MsG0880047090.01	MsG0880047654.01	0.819340	1.222409e-52	9.380301e-50

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047654.01.T01	MTR_4g132230	94.286	70	3	1	1	69	40	109	6.60e-42	132
MsG0880047654.01.T01	MTR_2g023440	49.351	77	24	3	1	62	1	77	3.40e-13	58.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047654.01.T01	AT1G62045	49.206	63	29	2	1	62	1	61	4.95e-13	57.8
MsG0880047654.01.T01	AT1G11740	49.231	65	30	2	1	62	573	637	3.01e-11	57.0

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Find 22 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACACCAACCAAGCAAC+TGG	0.372401	8:-89259810	MsG0880047654.01.T01:CDS
AAGACACCAACCAAGCAACT+GGG	0.633568	8:-89259809	MsG0880047654.01.T01:CDS
ACAGTTTGAGGCAGTTGCTT+TGG	0.357175	8:+89259862	None:intergenic
ACCAAGTTGGGCAGATCAAT+GGG	0.402298	8:-89259978	MsG0880047654.01.T01:CDS
AGCAAGAAGAGCAAGAATCC+AGG	0.523096	8:-89259899	MsG0880047654.01.T01:CDS
CAACCAAGCAACTGGGTTAA+TGG	0.264966	8:-89259802	None:intergenic
CAATGGGGTGCTGGTGGTTT+TGG	0.215218	8:-89259962	MsG0880047654.01.T01:CDS
CACCAAGTTGGGCAGATCAA+TGG	0.454043	8:-89259979	MsG0880047654.01.T01:CDS
CATTAACCCAGTTGCTTGGT+TGG	0.384881	8:+89259803	None:intergenic
CCAAGTTGGGCAGATCAATG+GGG	0.727100	8:-89259977	MsG0880047654.01.T01:CDS

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TAACCACTTTACACAGTTTG+AGG	+	Chr8:89259950-89259969	None:intergenic	35.0%
	AAAGACACCAACCAAGCAAC+TGG	-	Chr8:89259987-89260006	MsG0880047654.01.T01:CDS	45.0%
	AAGACACCAACCAAGCAACT+GGG	-	Chr8:89259988-89260007	MsG0880047654.01.T01:CDS	45.0%
	AGCAAGAAGAGCAAGAATCC+AGG	-	Chr8:89259898-89259917	MsG0880047654.01.T01:CDS	45.0%
	CTGCCTCAAACTGTGTAAAG+TGG	-	Chr8:89259944-89259963	MsG0880047654.01.T01:CDS	45.0%
	CCCCATTGATCTGCCCAACT+TGG	+	Chr8:89259823-89259842	None:intergenic	55.0%
	TGGGCAGATCAATGGGGTGC+TGG	-	Chr8:89259826-89259845	MsG0880047654.01.T01:CDS	60.0%
!	TTGGTGTCTTTCTCTTGCAA+AGG	+	Chr8:89259978-89259997	None:intergenic	40.0%
!	ACAGTTTGAGGCAGTTGCTT+TGG	+	Chr8:89259938-89259957	None:intergenic	45.0%
!	ACCAAGTTGGGCAGATCAAT+GGG	-	Chr8:89259819-89259838	MsG0880047654.01.T01:CDS	45.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	89259805	89260014	89259805	ID=MsG0880047654.01;Name=MsG0880047654.01
Chr8	mRNA	89259805	89260014	89259805	ID=MsG0880047654.01.T01;Parent=MsG0880047654.01;Name=MsG0880047654.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|69
Chr8	exon	89259805	89260014	89259805	ID=MsG0880047654.01.T01:exon:38819;Parent=MsG0880047654.01.T01
Chr8	CDS	89259805	89260014	89259805	ID=MsG0880047654.01.T01:cds;Parent=MsG0880047654.01.T01

Gene Sequence	>MsG0880047654.01.T01 ATGGCAACGGAGGCACCAAGTTGGGCAGATCAATGGGGTGCTGGTGGTTTTGGTGCAATGGAAGATGAGAATACTAGATCACAAAATGATACAAGCAAGAAGAGCAAGAATCCAGGTGCTTTATGCAAAGCCAAAGCAACTGCCTCAAACTGTGTAAAGTGGTTAAAAAGCCTTTGCAAGAGAAAGACACCAACCAAGCAACTGGGTTAA
Protein sequence	>MsG0880047654.01.T01 MATEAPSWADQWGAGGFGAMEDENTRSQNDTSKKSKNPGALCKAKATASNCVKWLKSLCKRKTPTKQLG*