Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024145.01.T01 | XP_003610728.1 | 96.063 | 127 | 4 | 1 | 1 | 126 | 1 | 127 | 2.45E-83 | 250 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024145.01.T01 | G7K3N3 | 96.063 | 127 | 4 | 1 | 1 | 126 | 1 | 127 | 1.17e-83 | 250 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180006253.01 | MsG0580024145.01 | 0.813949 | 1.975236e-51 | 1.307871e-48 |
| MsG0380014646.01 | MsG0580024145.01 | -0.801569 | 8.466047e-49 | 4.056360e-46 |
| MsG0380017200.01 | MsG0580024145.01 | 0.812909 | 3.344077e-51 | 2.152724e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024145.01.T01 | MTR_5g006370 | 96.063 | 127 | 4 | 1 | 1 | 126 | 1 | 127 | 2.96e-87 | 250 |
| MsG0580024145.01.T01 | MTR_5g006370 | 96.063 | 127 | 4 | 1 | 1 | 126 | 1 | 127 | 4.47e-87 | 249 |
| MsG0580024145.01.T01 | MTR_5g006370 | 94.949 | 99 | 4 | 1 | 29 | 126 | 1 | 99 | 3.96e-65 | 193 |
| MsG0580024145.01.T01 | MTR_5g006360 | 66.406 | 128 | 36 | 3 | 6 | 126 | 3 | 130 | 3.23e-57 | 174 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024145.01.T01 | AT2G32090 | 52.672 | 131 | 46 | 4 | 7 | 126 | 2 | 127 | 3.37e-47 | 149 |
| MsG0580024145.01.T01 | AT2G32090 | 50.459 | 109 | 38 | 4 | 29 | 126 | 2 | 105 | 6.19e-35 | 117 |
| MsG0580024145.01.T01 | AT2G28420 | 29.839 | 124 | 76 | 3 | 7 | 125 | 19 | 136 | 8.72e-12 | 59.7 |
Find 43 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTACCTCTTGGTAGAATT+TGG | 0.165781 | 5:+1313910 | None:intergenic |
| TAGGGTTATCCAACGAAATT+AGG | 0.180713 | 5:-1314073 | MsG0580024145.01.T01:five_prime_UTR |
| AGAAAAGATTTCTATTGATT+TGG | 0.249533 | 5:+1314018 | None:intergenic |
| TATTGATTTGGAAACAATAA+TGG | 0.294612 | 5:+1314030 | None:intergenic |
| TTGGACAGTTCAAGATTATA+TGG | 0.312649 | 5:-1313686 | MsG0580024145.01.T01:CDS |
| GAATGAGTTGTCAGATGTTT+GGG | 0.367566 | 5:-1313734 | MsG0580024145.01.T01:intron |
| ATCTTGAACTGTCCAAAGTC+AGG | 0.387838 | 5:+1313693 | None:intergenic |
| TGAATGAGTTGTCAGATGTT+TGG | 0.396436 | 5:-1313735 | MsG0580024145.01.T01:intron |
| ATATGAGAGGGGTCTTTAAC+AGG | 0.405224 | 5:+1313588 | None:intergenic |
| CGGTGGACTCTCGAGCTATA+TGG | 0.414336 | 5:+1313948 | None:intergenic |
| TCTAGAAAAGATAGAAGAAA+TGG | 0.416833 | 5:-1313996 | MsG0580024145.01.T01:exon |
| TTGTCAGATGTTTGGGTTTG+AGG | 0.424986 | 5:-1313727 | MsG0580024145.01.T01:intron |
| GAAGTTGAGACCCCTGACTT+TGG | 0.454246 | 5:-1313705 | MsG0580024145.01.T01:CDS |
| AGTTAGTCTTAATTACCATC+AGG | 0.460600 | 5:+1313329 | None:intergenic |
| TTTCAAATCATCACATGGGT+AGG | 0.475491 | 5:-1314092 | None:intergenic |
| GTTGTGAAAGGAATGAAGAT+TGG | 0.479221 | 5:+1313533 | None:intergenic |
| ATCTAGCACCCTAATTTCGT+TGG | 0.488283 | 5:+1314064 | None:intergenic |
| AGATGGAGAAGCAGAGATGA+TGG | 0.508377 | 5:+1313556 | None:intergenic |
| TTCAAATCATCACATGGGTA+GGG | 0.520517 | 5:-1314091 | None:intergenic |
| AGAGGGGTCTTTAACAGGAA+TGG | 0.522033 | 5:+1313593 | None:intergenic |
| TTCCTTTCACAACACTCTCA+AGG | 0.527218 | 5:-1313523 | MsG0580024145.01.T01:intron |
| AGGGTTATCCAACGAAATTA+GGG | 0.528572 | 5:-1314072 | MsG0580024145.01.T01:five_prime_UTR |
| GAAGAAATGGCCGCAGCAGA+AGG | 0.534245 | 5:-1313983 | MsG0580024145.01.T01:exon |
| TCTTGAACTGTCCAAAGTCA+GGG | 0.541425 | 5:+1313694 | None:intergenic |
| TGGATAAGGAAGGATGAAGA+AGG | 0.546868 | 5:+1313654 | None:intergenic |
| TTAACAGGAATGGATGATGA+TGG | 0.555627 | 5:+1313603 | None:intergenic |
| CATACAACGCAGCAACGAGC+TGG | 0.557736 | 5:-1313628 | MsG0580024145.01.T01:CDS |
| AACCTTGAGAGTGTTGTGAA+AGG | 0.570775 | 5:+1313521 | None:intergenic |
| AAAGGAATGAAGATTGGAGA+TGG | 0.571006 | 5:+1313539 | None:intergenic |
| GTTAGTCTTAATTACCATCA+GGG | 0.586779 | 5:+1313330 | None:intergenic |
| TGGCCTAAGCGGATATGAGA+GGG | 0.586943 | 5:+1313576 | None:intergenic |
| CGTTGCTGCGTTGTATGAGA+TGG | 0.602849 | 5:+1313634 | None:intergenic |
| TGCGTTGTATGAGATGGATA+AGG | 0.613103 | 5:+1313640 | None:intergenic |
| AGCAGAGATGATGGCCTAAG+CGG | 0.614263 | 5:+1313565 | None:intergenic |
| ATGGCCTAAGCGGATATGAG+AGG | 0.629456 | 5:+1313575 | None:intergenic |
| TTGTATGAGATGGATAAGGA+AGG | 0.635954 | 5:+1313644 | None:intergenic |
| GACCCCTCTCATATCCGCTT+AGG | 0.637582 | 5:-1313579 | MsG0580024145.01.T01:CDS |
| TAAGGAAGGATGAAGAAGGA+AGG | 0.647624 | 5:+1313658 | None:intergenic |
| ACTCTCCAAATTCTACCAAG+AGG | 0.647769 | 5:-1313915 | MsG0580024145.01.T01:intron |
| CTTGAACTGTCCAAAGTCAG+GGG | 0.685610 | 5:+1313695 | None:intergenic |
| TGATTTGGAAACAATAATGG+TGG | 0.685697 | 5:+1314033 | None:intergenic |
| CAGACTCACTCCTTCTGCTG+CGG | 0.692086 | 5:+1313973 | None:intergenic |
| GGCCTAAGCGGATATGAGAG+GGG | 0.694889 | 5:+1313577 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTAAATTATTATCTGTG+TGG | - | Chr5:1313610-1313629 | MsG0580024145.01.T01:CDS | 15.0% |
| !!! | AGAAAAGATTTCTATTGATT+TGG | + | Chr5:1313402-1313421 | None:intergenic | 20.0% |
| !!! | TATTGATTTGGAAACAATAA+TGG | + | Chr5:1313390-1313409 | None:intergenic | 20.0% |
| ! | TCTAGAAAAGATAGAAGAAA+TGG | - | Chr5:1313421-1313440 | MsG0580024145.01.T01:intron | 25.0% |
| ! | TTAAATACTAACTTACCTCT+TGG | + | Chr5:1313520-1313539 | None:intergenic | 25.0% |
| !!! | CACAAGTCATTTTTTTATAC+AGG | - | Chr5:1314003-1314022 | MsG0580024145.01.T01:five_prime_UTR | 25.0% |
| !!! | CATTTTTTTATACAGGACAA+AGG | - | Chr5:1314010-1314029 | MsG0580024145.01.T01:five_prime_UTR | 25.0% |
| AAACACAAATCTAACAACAG+TGG | + | Chr5:1313939-1313958 | None:intergenic | 30.0% | |
| ATTAACAAGTGATATGAAGC+AGG | + | Chr5:1313661-1313680 | None:intergenic | 30.0% | |
| TTGGACAGTTCAAGATTATA+TGG | - | Chr5:1313731-1313750 | MsG0580024145.01.T01:intron | 30.0% | |
| ! | TGATTTGGAAACAATAATGG+TGG | + | Chr5:1313387-1313406 | None:intergenic | 30.0% |
| AAAGGAATGAAGATTGGAGA+TGG | + | Chr5:1313881-1313900 | None:intergenic | 35.0% | |
| ACTTACCTCTTGGTAGAATT+TGG | + | Chr5:1313510-1313529 | None:intergenic | 35.0% | |
| AGGGTTATCCAACGAAATTA+GGG | - | Chr5:1313345-1313364 | MsG0580024145.01.T01:CDS | 35.0% | |
| GAATGAGTTGTCAGATGTTT+GGG | - | Chr5:1313683-1313702 | MsG0580024145.01.T01:CDS | 35.0% | |
| GTTGTGAAAGGAATGAAGAT+TGG | + | Chr5:1313887-1313906 | None:intergenic | 35.0% | |
| TAGGGTTATCCAACGAAATT+AGG | - | Chr5:1313344-1313363 | MsG0580024145.01.T01:CDS | 35.0% | |
| TGAATGAGTTGTCAGATGTT+TGG | - | Chr5:1313682-1313701 | MsG0580024145.01.T01:CDS | 35.0% | |
| TTAACAGGAATGGATGATGA+TGG | + | Chr5:1313817-1313836 | None:intergenic | 35.0% | |
| TTGTATGAGATGGATAAGGA+AGG | + | Chr5:1313776-1313795 | None:intergenic | 35.0% | |
| ! | TTTGGAGAGTCTTTTTACGT+CGG | + | Chr5:1313492-1313511 | None:intergenic | 35.0% |
| !!! | GAGACTTTTGAGACTACTAA+TGG | - | Chr5:1314037-1314056 | MsG0580024145.01.T01:five_prime_UTR | 35.0% |
| ACTCTCCAAATTCTACCAAG+AGG | - | Chr5:1313502-1313521 | MsG0580024145.01.T01:intron | 40.0% | |
| AGGAACACATACACGAACAT+CGG | + | Chr5:1313641-1313660 | None:intergenic | 40.0% | |
| ATATGAGAGGGGTCTTTAAC+AGG | + | Chr5:1313832-1313851 | None:intergenic | 40.0% | |
| ATCTAGCACCCTAATTTCGT+TGG | + | Chr5:1313356-1313375 | None:intergenic | 40.0% | |
| ATCTTGAACTGTCCAAAGTC+AGG | + | Chr5:1313727-1313746 | None:intergenic | 40.0% | |
| TAAGGAAGGATGAAGAAGGA+AGG | + | Chr5:1313762-1313781 | None:intergenic | 40.0% | |
| TCTTGAACTGTCCAAAGTCA+GGG | + | Chr5:1313726-1313745 | None:intergenic | 40.0% | |
| TGCGTTGTATGAGATGGATA+AGG | + | Chr5:1313780-1313799 | None:intergenic | 40.0% | |
| TGGATAAGGAAGGATGAAGA+AGG | + | Chr5:1313766-1313785 | None:intergenic | 40.0% | |
| TTCCTTTCACAACACTCTCA+AGG | - | Chr5:1313894-1313913 | MsG0580024145.01.T01:intron | 40.0% | |
| ! | GTTTTCTTCTATGACCCTGA+TGG | - | Chr5:1314073-1314092 | MsG0580024145.01.T01:five_prime_UTR | 40.0% |
| ! | TTGTCAGATGTTTGGGTTTG+AGG | - | Chr5:1313690-1313709 | MsG0580024145.01.T01:CDS | 40.0% |
| !! | AACCTTGAGAGTGTTGTGAA+AGG | + | Chr5:1313899-1313918 | None:intergenic | 40.0% |
| !! | CTAGATGAGTGTCACTTCAT+AGG | + | Chr5:1313543-1313562 | None:intergenic | 40.0% |
| AGAGGGGTCTTTAACAGGAA+TGG | + | Chr5:1313827-1313846 | None:intergenic | 45.0% | |
| AGATGGAGAAGCAGAGATGA+TGG | + | Chr5:1313864-1313883 | None:intergenic | 45.0% | |
| CAACACAACACTGACACATG+CGG | - | Chr5:1313571-1313590 | MsG0580024145.01.T01:CDS | 45.0% | |
| CTTGAACTGTCCAAAGTCAG+GGG | + | Chr5:1313725-1313744 | None:intergenic | 45.0% | |
| ATGGCCTAAGCGGATATGAG+AGG | + | Chr5:1313845-1313864 | None:intergenic | 50.0% | |
| CGCATGTGTCAGTGTTGTGT+TGG | + | Chr5:1313573-1313592 | None:intergenic | 50.0% | |
| CGTTGCTGCGTTGTATGAGA+TGG | + | Chr5:1313786-1313805 | None:intergenic | 50.0% | |
| GAAGTTGAGACCCCTGACTT+TGG | - | Chr5:1313712-1313731 | MsG0580024145.01.T01:CDS | 50.0% | |
| TGGCCTAAGCGGATATGAGA+GGG | + | Chr5:1313844-1313863 | None:intergenic | 50.0% | |
| ! | AGCAGAGATGATGGCCTAAG+CGG | + | Chr5:1313855-1313874 | None:intergenic | 50.0% |
| ! | GGAGAGTCTTTTTACGTCGG+TGG | + | Chr5:1313489-1313508 | None:intergenic | 50.0% |
| CAGACTCACTCCTTCTGCTG+CGG | + | Chr5:1313447-1313466 | None:intergenic | 55.0% | |
| CATACAACGCAGCAACGAGC+TGG | - | Chr5:1313789-1313808 | MsG0580024145.01.T01:intron | 55.0% | |
| GAAGAAATGGCCGCAGCAGA+AGG | - | Chr5:1313434-1313453 | MsG0580024145.01.T01:intron | 55.0% | |
| GACCCCTCTCATATCCGCTT+AGG | - | Chr5:1313838-1313857 | MsG0580024145.01.T01:intron | 55.0% | |
| GGCCTAAGCGGATATGAGAG+GGG | + | Chr5:1313843-1313862 | None:intergenic | 55.0% | |
| ! | CGGTGGACTCTCGAGCTATA+TGG | + | Chr5:1313472-1313491 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 1313337 | 1314102 | 1313337 | ID=MsG0580024145.01;Name=MsG0580024145.01 |
| Chr5 | mRNA | 1313337 | 1314102 | 1313337 | ID=MsG0580024145.01.T01;Parent=MsG0580024145.01;Name=MsG0580024145.01.T01;_AED=0.45;_eAED=0.45;_QI=103|1|0.66|1|1|1|3|0|126 |
| Chr5 | exon | 1313337 | 1313414 | 1313337 | ID=MsG0580024145.01.T01:exon:398;Parent=MsG0580024145.01.T01 |
| Chr5 | exon | 1313524 | 1313742 | 1313524 | ID=MsG0580024145.01.T01:exon:397;Parent=MsG0580024145.01.T01 |
| Chr5 | exon | 1313916 | 1314102 | 1313916 | ID=MsG0580024145.01.T01:exon:396;Parent=MsG0580024145.01.T01 |
| Chr5 | five_prime_UTR | 1314000 | 1314102 | 1314000 | ID=MsG0580024145.01.T01:five_prime_utr;Parent=MsG0580024145.01.T01 |
| Chr5 | CDS | 1313916 | 1313999 | 1313916 | ID=MsG0580024145.01.T01:cds;Parent=MsG0580024145.01.T01 |
| Chr5 | CDS | 1313524 | 1313742 | 1313524 | ID=MsG0580024145.01.T01:cds;Parent=MsG0580024145.01.T01 |
| Chr5 | CDS | 1313337 | 1313414 | 1313337 | ID=MsG0580024145.01.T01:cds;Parent=MsG0580024145.01.T01 |
| Gene Sequence |
| Protein sequence |
