Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024291.01.T01 | XP_003610916.1 | 93.827 | 81 | 0 | 1 | 6 | 86 | 38 | 113 | 5.00E-46 | 153 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024291.01.T01 | Q9T0G2 | 90 | 80 | 3 | 1 | 6 | 85 | 38 | 112 | 4.03E-46 | 146 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024291.01.T01 | A0A371GM79 | 90.123 | 81 | 3 | 1 | 6 | 86 | 38 | 113 | 1.84e-45 | 156 |
| MsG0580024291.01.T01 | G7K7W9 | 93.827 | 81 | 0 | 1 | 6 | 86 | 38 | 113 | 2.39e-46 | 153 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001110.01 | MsG0580024291.01 | 0.802960 | 4.376735e-49 | 2.172639e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024291.01.T01 | MTR_5g008460 | 93.827 | 81 | 0 | 1 | 6 | 86 | 38 | 113 | 6.06e-50 | 153 |
| MsG0580024291.01.T01 | MTR_7g080803 | 87.654 | 81 | 5 | 1 | 6 | 86 | 38 | 113 | 1.29e-46 | 144 |
| MsG0580024291.01.T01 | MTR_7g080793 | 75.000 | 76 | 14 | 1 | 6 | 81 | 37 | 107 | 3.22e-35 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024291.01.T01 | AT4G10040 | 90.000 | 80 | 3 | 1 | 6 | 85 | 38 | 112 | 4.11e-47 | 146 |
| MsG0580024291.01.T01 | AT1G22840 | 86.250 | 80 | 6 | 1 | 6 | 85 | 38 | 112 | 2.32e-44 | 139 |
| MsG0580024291.01.T01 | AT1G22840 | 81.481 | 54 | 10 | 0 | 6 | 59 | 38 | 91 | 5.06e-26 | 92.4 |
Find 44 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATTCTTATTTGGTTAAT+TGG | 0.120545 | 5:+3042881 | MsG0580024291.01.T01:three_prime_UTR |
| TTGATTGAGCAACCAATATT+TGG | 0.238749 | 5:-3042838 | None:intergenic |
| GAGTTGGAAACATTCTTATT+TGG | 0.269770 | 5:+3042873 | MsG0580024291.01.T01:three_prime_UTR |
| CCCAATCTGAATGGTTTGTT+TGG | 0.305862 | 5:+3041890 | MsG0580024291.01.T01:CDS |
| AGAAGTTTGCACTGTATCTT+TGG | 0.306075 | 5:+3042747 | MsG0580024291.01.T01:three_prime_UTR |
| AATAAGATCAGCACGTTCTT+GGG | 0.344346 | 5:-3042615 | None:intergenic |
| CAATAAGATCAGCACGTTCT+TGG | 0.348957 | 5:-3042616 | None:intergenic |
| ACAAGAACATGGCTGTGAAT+TGG | 0.349587 | 5:+3041957 | MsG0580024291.01.T01:CDS |
| TTATTTGCTTAACCCCAAAA+AGG | 0.352975 | 5:+3042000 | MsG0580024291.01.T01:CDS |
| CAAGAACATGGCTGTGAATT+GGG | 0.354427 | 5:+3041958 | MsG0580024291.01.T01:CDS |
| AGCTGCCGAGTAGGAATATC+CGG | 0.375428 | 5:-3041933 | None:intergenic |
| GGTTTGTTTGGAAGGCAATC+AGG | 0.375977 | 5:+3041902 | MsG0580024291.01.T01:CDS |
| GCTCTTGTGCAGTATATTCC+AGG | 0.394249 | 5:+3042560 | MsG0580024291.01.T01:intron |
| TTCTTGGGGTTTCTTTAGAC+CGG | 0.417289 | 5:-3042600 | None:intergenic |
| TCCAAACAAACCATTCAGAT+TGG | 0.424075 | 5:-3041891 | None:intergenic |
| GCATACTTTAATTATTTGCT+TGG | 0.436437 | 5:+3042906 | MsG0580024291.01.T01:three_prime_UTR |
| TTTGCCTTGTTGATAATCTC+AGG | 0.439423 | 5:+3041866 | None:intergenic |
| ATCTGAATGGTTTGTTTGGA+AGG | 0.441498 | 5:+3041894 | MsG0580024291.01.T01:CDS |
| GCAAATAAAGTAGGCTACAT+TGG | 0.452206 | 5:+3042769 | MsG0580024291.01.T01:three_prime_UTR |
| AAGATTTGCCTGATCACAAA+AGG | 0.456940 | 5:-3043054 | None:intergenic |
| TGCTTAGCTCAATAAGTACT+AGG | 0.471021 | 5:+3042976 | MsG0580024291.01.T01:three_prime_UTR |
| GGGACCAAGATGGTATTTCC+CGG | 0.471291 | 5:+3042581 | MsG0580024291.01.T01:CDS |
| TCAAATTATCTATGAAGAGT+TGG | 0.473907 | 5:+3042857 | MsG0580024291.01.T01:three_prime_UTR |
| CCAAACAAACCATTCAGATT+GGG | 0.484079 | 5:-3041890 | None:intergenic |
| AGGCAATCAGGCACTACTCC+CGG | 0.508362 | 5:+3041914 | MsG0580024291.01.T01:CDS |
| GTATCTTTGGCAAATAAAGT+AGG | 0.523100 | 5:+3042760 | MsG0580024291.01.T01:three_prime_UTR |
| GGAAATACCATCTTGGTCCC+TGG | 0.530773 | 5:-3042578 | None:intergenic |
| AGCTCAATAAGTACTAGGAC+GGG | 0.535789 | 5:+3042981 | MsG0580024291.01.T01:three_prime_UTR |
| TAGACCGGGAAATACCATCT+TGG | 0.536477 | 5:-3042585 | None:intergenic |
| ATCTCAGGACCCAATCTGAA+TGG | 0.539851 | 5:+3041881 | MsG0580024291.01.T01:CDS |
| CTCTTGTGCAGTATATTCCA+GGG | 0.564315 | 5:+3042561 | MsG0580024291.01.T01:intron |
| GTTCTTGTTAGCTGCCGAGT+AGG | 0.576933 | 5:-3041942 | None:intergenic |
| AATAAGTACTAGGACGGGAA+AGG | 0.578550 | 5:+3042986 | MsG0580024291.01.T01:three_prime_UTR |
| TCTTGGGGTTTCTTTAGACC+GGG | 0.586837 | 5:-3042599 | None:intergenic |
| CTCGGCAGCTAACAAGAACA+TGG | 0.601333 | 5:+3041946 | MsG0580024291.01.T01:CDS |
| ATAAGATCAGCACGTTCTTG+GGG | 0.603518 | 5:-3042614 | None:intergenic |
| TAGCTCAATAAGTACTAGGA+CGG | 0.616217 | 5:+3042980 | MsG0580024291.01.T01:three_prime_UTR |
| TGATCTTATTGCATATCTGA+AGG | 0.616592 | 5:+3042628 | MsG0580024291.01.T01:CDS |
| TACTCCCGGATATTCCTACT+CGG | 0.628766 | 5:+3041928 | MsG0580024291.01.T01:CDS |
| AATATATAATCACTGTGCAG+TGG | 0.632456 | 5:-3042654 | None:intergenic |
| GCTGCCGAGTAGGAATATCC+GGG | 0.639197 | 5:-3041932 | None:intergenic |
| GTATATTCCAGGGACCAAGA+TGG | 0.661039 | 5:+3042571 | MsG0580024291.01.T01:intron |
| GAACATGGCTGTGAATTGGG+AGG | 0.673762 | 5:+3041961 | MsG0580024291.01.T01:CDS |
| GGGTCCTGAGATTATCAACA+AGG | 0.675341 | 5:-3041870 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATTTAGTAGTATTGATT+AGG | - | Chr5:3042350-3042369 | None:intergenic | 15.0% |
| !!! | AACATTCTTATTTGGTTAAT+TGG | + | Chr5:3042881-3042900 | MsG0580024291.01.T01:three_prime_UTR | 20.0% |
| !!! | TTGTTTTTCTTTTCGTTTAT+AGG | + | Chr5:3042387-3042406 | MsG0580024291.01.T01:intron | 20.0% |
| ! | ATTAGCATATTACCACAATA+TGG | - | Chr5:3042482-3042501 | None:intergenic | 25.0% |
| ! | GCATACTTTAATTATTTGCT+TGG | + | Chr5:3042906-3042925 | MsG0580024291.01.T01:three_prime_UTR | 25.0% |
| ! | TCAAATTATCTATGAAGAGT+TGG | + | Chr5:3042857-3042876 | MsG0580024291.01.T01:three_prime_UTR | 25.0% |
| !! | AGATTGTATTGTACCTTTTT+GGG | - | Chr5:3042016-3042035 | None:intergenic | 25.0% |
| !!! | TTTCTAAGATTTCCATATTG+TGG | + | Chr5:3042417-3042436 | MsG0580024291.01.T01:intron | 25.0% |
| AAACGAAAAGAAAAACAAGC+AGG | - | Chr5:3042385-3042404 | None:intergenic | 30.0% | |
| AACGAAAAGAAAAACAAGCA+GGG | - | Chr5:3042384-3042403 | None:intergenic | 30.0% | |
| AATATATAATCACTGTGCAG+TGG | - | Chr5:3042657-3042676 | None:intergenic | 30.0% | |
| CATAGATCAGTAACAAATTG+TGG | - | Chr5:3042537-3042556 | None:intergenic | 30.0% | |
| GAGTTGGAAACATTCTTATT+TGG | + | Chr5:3042873-3042892 | MsG0580024291.01.T01:three_prime_UTR | 30.0% | |
| GTATCTTTGGCAAATAAAGT+AGG | + | Chr5:3042760-3042779 | MsG0580024291.01.T01:three_prime_UTR | 30.0% | |
| TGATCTTATTGCATATCTGA+AGG | + | Chr5:3042628-3042647 | MsG0580024291.01.T01:CDS | 30.0% | |
| TGGTAATATGCTACGTTTAT+AGG | + | Chr5:3042437-3042456 | MsG0580024291.01.T01:intron | 30.0% | |
| TTATTTGCTTAACCCCAAAA+AGG | + | Chr5:3042000-3042019 | MsG0580024291.01.T01:CDS | 30.0% | |
| TTGATTGAGCAACCAATATT+TGG | - | Chr5:3042841-3042860 | None:intergenic | 30.0% | |
| ! | ACTTTTCAATGAACACTTTC+AGG | + | Chr5:3042103-3042122 | MsG0580024291.01.T01:intron | 30.0% |
| ! | CAAAAAGGTACAATACAATC+TGG | + | Chr5:3042015-3042034 | MsG0580024291.01.T01:CDS | 30.0% |
| ! | CAGATTGTATTGTACCTTTT+TGG | - | Chr5:3042017-3042036 | None:intergenic | 30.0% |
| ! | GATTGTATTGTACCTTTTTG+GGG | - | Chr5:3042015-3042034 | None:intergenic | 30.0% |
| AATAAGATCAGCACGTTCTT+GGG | - | Chr5:3042618-3042637 | None:intergenic | 35.0% | |
| ACCTTCACATATAACAAGCA+AGG | + | Chr5:3042236-3042255 | MsG0580024291.01.T01:intron | 35.0% | |
| AGAAGTTTGCACTGTATCTT+TGG | + | Chr5:3042747-3042766 | MsG0580024291.01.T01:three_prime_UTR | 35.0% | |
| AGATCAGTAACAAATTGTGG+TGG | - | Chr5:3042534-3042553 | None:intergenic | 35.0% | |
| AGTTGTAAGCTAATCCAAAC+TGG | + | Chr5:3042310-3042329 | MsG0580024291.01.T01:intron | 35.0% | |
| CCAAACAAACCATTCAGATT+GGG | - | Chr5:3041893-3041912 | None:intergenic | 35.0% | |
| CGTAGCATATTACCACAATA+TGG | - | Chr5:3042432-3042451 | None:intergenic | 35.0% | |
| CTCAAAAAGCTGCCAAATAT+TGG | + | Chr5:3042826-3042845 | MsG0580024291.01.T01:three_prime_UTR | 35.0% | |
| GCAAATAAAGTAGGCTACAT+TGG | + | Chr5:3042769-3042788 | MsG0580024291.01.T01:three_prime_UTR | 35.0% | |
| TAGCTCAATAAGTACTAGGA+CGG | + | Chr5:3042980-3042999 | MsG0580024291.01.T01:three_prime_UTR | 35.0% | |
| TCCAAACAAACCATTCAGAT+TGG | - | Chr5:3041894-3041913 | None:intergenic | 35.0% | |
| TGCTTAGCTCAATAAGTACT+AGG | + | Chr5:3042976-3042995 | MsG0580024291.01.T01:three_prime_UTR | 35.0% | |
| TTGATTAGGAGTGAAGCATA+TGG | - | Chr5:3042336-3042355 | None:intergenic | 35.0% | |
| !! | ATCTGAATGGTTTGTTTGGA+AGG | + | Chr5:3041894-3041913 | MsG0580024291.01.T01:CDS | 35.0% |
| !! | TTGCTTGTTATATGTGAAGG+TGG | - | Chr5:3042237-3042256 | None:intergenic | 35.0% |
| !!! | ACAGTGTGATTTTGAAGTTC+TGG | + | Chr5:3042694-3042713 | MsG0580024291.01.T01:three_prime_UTR | 35.0% |
| AATAAGTACTAGGACGGGAA+AGG | + | Chr5:3042986-3043005 | MsG0580024291.01.T01:three_prime_UTR | 40.0% | |
| ACAAGAACATGGCTGTGAAT+TGG | + | Chr5:3041957-3041976 | MsG0580024291.01.T01:CDS | 40.0% | |
| AGCTCAATAAGTACTAGGAC+GGG | + | Chr5:3042981-3043000 | MsG0580024291.01.T01:three_prime_UTR | 40.0% | |
| ATAAGATCAGCACGTTCTTG+GGG | - | Chr5:3042617-3042636 | None:intergenic | 40.0% | |
| CAAGAACATGGCTGTGAATT+GGG | + | Chr5:3041958-3041977 | MsG0580024291.01.T01:CDS | 40.0% | |
| CAATAAGATCAGCACGTTCT+TGG | - | Chr5:3042619-3042638 | None:intergenic | 40.0% | |
| CTCTTGTGCAGTATATTCCA+GGG | + | Chr5:3042561-3042580 | MsG0580024291.01.T01:intron | 40.0% | |
| GTTATATGTGAAGGTGGCAA+TGG | - | Chr5:3042231-3042250 | None:intergenic | 40.0% | |
| TTCTTGGGGTTTCTTTAGAC+CGG | - | Chr5:3042603-3042622 | None:intergenic | 40.0% | |
| !! | CCCAATCTGAATGGTTTGTT+TGG | + | Chr5:3041890-3041909 | MsG0580024291.01.T01:CDS | 40.0% |
| !! | GCCTTGCTTGTTATATGTGA+AGG | - | Chr5:3042240-3042259 | None:intergenic | 40.0% |
| !!! | GTGTGATTTTGAAGTTCTGG+AGG | + | Chr5:3042697-3042716 | MsG0580024291.01.T01:three_prime_UTR | 40.0% |
| AGTGAAGCATATGGCCAGTT+TGG | - | Chr5:3042327-3042346 | None:intergenic | 45.0% | |
| ATCTCAGGACCCAATCTGAA+TGG | + | Chr5:3041881-3041900 | MsG0580024291.01.T01:CDS | 45.0% | |
| GCTCTTGTGCAGTATATTCC+AGG | + | Chr5:3042560-3042579 | MsG0580024291.01.T01:intron | 45.0% | |
| GTATATTCCAGGGACCAAGA+TGG | + | Chr5:3042571-3042590 | MsG0580024291.01.T01:intron | 45.0% | |
| TACTCCCGGATATTCCTACT+CGG | + | Chr5:3041928-3041947 | MsG0580024291.01.T01:CDS | 45.0% | |
| TAGACCGGGAAATACCATCT+TGG | - | Chr5:3042588-3042607 | None:intergenic | 45.0% | |
| TCTTGGGGTTTCTTTAGACC+GGG | - | Chr5:3042602-3042621 | None:intergenic | 45.0% | |
| ! | GGTTTGTTTGGAAGGCAATC+AGG | + | Chr5:3041902-3041921 | MsG0580024291.01.T01:CDS | 45.0% |
| AGCTGCCGAGTAGGAATATC+CGG | - | Chr5:3041936-3041955 | None:intergenic | 50.0% | |
| CTCGGCAGCTAACAAGAACA+TGG | + | Chr5:3041946-3041965 | MsG0580024291.01.T01:CDS | 50.0% | |
| GAACATGGCTGTGAATTGGG+AGG | + | Chr5:3041961-3041980 | MsG0580024291.01.T01:CDS | 50.0% | |
| GGAAATACCATCTTGGTCCC+TGG | - | Chr5:3042581-3042600 | None:intergenic | 50.0% | |
| GGGACCAAGATGGTATTTCC+CGG | + | Chr5:3042581-3042600 | MsG0580024291.01.T01:CDS | 50.0% | |
| GTTCTTGTTAGCTGCCGAGT+AGG | - | Chr5:3041945-3041964 | None:intergenic | 50.0% | |
| AGGCAATCAGGCACTACTCC+CGG | + | Chr5:3041914-3041933 | MsG0580024291.01.T01:CDS | 55.0% | |
| GCTGCCGAGTAGGAATATCC+GGG | - | Chr5:3041935-3041954 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 3041872 | 3043067 | 3041872 | ID=MsG0580024291.01;Name=MsG0580024291.01 |
| Chr5 | mRNA | 3041872 | 3043067 | 3041872 | ID=MsG0580024291.01.T01;Parent=MsG0580024291.01;Name=MsG0580024291.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0|0.5|1|1|1|2|400|86 |
| Chr5 | exon | 3041872 | 3042036 | 3041872 | ID=MsG0580024291.01.T01:exon:1934;Parent=MsG0580024291.01.T01 |
| Chr5 | exon | 3042572 | 3043067 | 3042572 | ID=MsG0580024291.01.T01:exon:1935;Parent=MsG0580024291.01.T01 |
| Chr5 | CDS | 3041872 | 3042036 | 3041872 | ID=MsG0580024291.01.T01:cds;Parent=MsG0580024291.01.T01 |
| Chr5 | CDS | 3042572 | 3042667 | 3042572 | ID=MsG0580024291.01.T01:cds;Parent=MsG0580024291.01.T01 |
| Chr5 | three_prime_UTR | 3042668 | 3043067 | 3042668 | ID=MsG0580024291.01.T01:three_prime_utr;Parent=MsG0580024291.01.T01 |
| Gene Sequence |
| Protein sequence |
