Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001110.01.T01 | XP_013466515.1 | 97.403 | 154 | 3 | 1 | 12 | 165 | 1 | 153 | 1.26E-90 | 271 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001110.01.T01 | A0A072VG23 | 97.403 | 154 | 3 | 1 | 12 | 165 | 1 | 153 | 6.02e-91 | 271 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048698.01 | MsG0180001110.01 | 0.807193 | 5.696280e-50 | 3.152601e-47 |
| MsG0180001110.01 | MsG0380013929.01 | 0.806352 | 8.576036e-50 | 4.644569e-47 |
| MsG0180001110.01 | MsG0380013931.01 | 0.824019 | 1.012654e-53 | 8.862898e-51 |
| MsG0180001110.01 | MsG0380013942.01 | 0.817911 | 2.578711e-52 | 1.902433e-49 |
| MsG0180001110.01 | MsG0380013945.01 | 0.802389 | 5.742879e-49 | 2.809762e-46 |
| MsG0180001110.01 | MsG0380013951.01 | 0.800627 | 1.319058e-48 | 6.171344e-46 |
| MsG0180001110.01 | MsG0380013957.01 | 0.800061 | 1.719697e-48 | 7.931150e-46 |
| MsG0180001110.01 | MsG0380013966.01 | 0.814880 | 1.229775e-51 | 8.350236e-49 |
| MsG0180001110.01 | MsG0380013967.01 | 0.800775 | 1.230109e-48 | 5.776740e-46 |
| MsG0180001110.01 | MsG0380013969.01 | 0.804317 | 2.289643e-49 | 1.176843e-46 |
| MsG0180001110.01 | MsG0380013977.01 | 0.801197 | 1.008615e-48 | 4.787587e-46 |
| MsG0180001110.01 | MsG0380013981.01 | 0.807216 | 5.632565e-50 | 3.119204e-47 |
| MsG0180001110.01 | MsG0580024291.01 | 0.802960 | 4.376735e-49 | 2.172639e-46 |
| MsG0180001110.01 | MsG0580027677.01 | 0.808768 | 2.633349e-50 | 1.518634e-47 |
| MsG0180001110.01 | MsG0780041296.01 | 0.804821 | 1.797334e-49 | 9.359707e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001110.01.T01 | MTR_1g030850 | 97.403 | 154 | 3 | 1 | 12 | 165 | 1 | 153 | 1.53e-94 | 271 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 66 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTGGATTCTTGCCACTTT+TGG | 0.164383 | 1:+16114038 | MsG0180001110.01.T01:CDS |
| CTTGATTCCATAGCATTAAT+TGG | 0.238500 | 1:-16113694 | None:intergenic |
| GGTGGGGAATTGTATATAAT+TGG | 0.272328 | 1:-16113772 | None:intergenic |
| TTCGCCGAGGGCGGTTTCTT+AGG | 0.303776 | 1:-16113880 | None:intergenic |
| TATTGAGCCTTCTAGTCCTT+TGG | 0.336447 | 1:+16114067 | MsG0180001110.01.T01:CDS |
| GCAAAACTCGCGCGGCCGCC+GGG | 0.375040 | 1:-16114018 | None:intergenic |
| CCACTGAAATTCACATCAAC+TGG | 0.377072 | 1:-16113982 | None:intergenic |
| ATTGGAGGTGGTGAATATAT+TGG | 0.391039 | 1:-16113754 | None:intergenic |
| AGCAAAACTCGCGCGGCCGC+CGG | 0.404307 | 1:-16114019 | None:intergenic |
| AATTCACATCAACTGGATAT+AGG | 0.414727 | 1:-16113975 | None:intergenic |
| AATTCCCATCAGAACCGTTA+TGG | 0.418191 | 1:+16113606 | MsG0180001110.01.T01:CDS |
| CTAATCACCAAAGGACTAGA+AGG | 0.430675 | 1:-16114074 | None:intergenic |
| GATGTGAATTTCAGTGGATC+AGG | 0.438016 | 1:+16113988 | MsG0180001110.01.T01:CDS |
| TACTCTTCCAATTAATGCTA+TGG | 0.439123 | 1:+16113687 | MsG0180001110.01.T01:CDS |
| CAAAACTCGCGCGGCCGCCG+GGG | 0.445668 | 1:-16114017 | None:intergenic |
| TTCCACTAAGAATCCTCAAT+TGG | 0.445827 | 1:+16113644 | MsG0180001110.01.T01:CDS |
| GGTGGTGAATAGTATACTAT+TGG | 0.449267 | 1:-16113847 | None:intergenic |
| GGATACACAATTGGTGGTGG+TGG | 0.456663 | 1:-16113790 | None:intergenic |
| GGTGGTGGTGGCGACGGAGA+TGG | 0.479323 | 1:-16113826 | None:intergenic |
| ATCCAATTGAGGATTCTTAG+TGG | 0.487330 | 1:-16113646 | None:intergenic |
| TTCTTAGGTGAAGGTGGTGG+TGG | 0.488561 | 1:-16113865 | None:intergenic |
| CTCAATAAACAAACCAAAAG+TGG | 0.492494 | 1:-16114051 | None:intergenic |
| GGCGACGGAGATGGTGGAGG+TGG | 0.497383 | 1:-16113817 | None:intergenic |
| GGAGATGGTGGAGGTGGTGA+TGG | 0.499789 | 1:-16113811 | None:intergenic |
| GGTGGCGACGGAGATGGTGG+AGG | 0.502153 | 1:-16113820 | None:intergenic |
| AGGATCCATAACGGTTCTGA+TGG | 0.507150 | 1:-16113611 | None:intergenic |
| GGTGGTGGCGACGGAGATGG+TGG | 0.508964 | 1:-16113823 | None:intergenic |
| TGTATACATGACTGGACCGC+CGG | 0.510813 | 1:+16113948 | MsG0180001110.01.T01:CDS |
| GGTCCAGTCATGTATACATA+TGG | 0.516775 | 1:-16113943 | None:intergenic |
| GTATACATGACTGGACCGCC+GGG | 0.517869 | 1:+16113949 | MsG0180001110.01.T01:CDS |
| GATGGTGATGATGGTGGTGG+AGG | 0.525256 | 1:-16113907 | None:intergenic |
| GATACACAATTGGTGGTGGT+GGG | 0.526844 | 1:-16113789 | None:intergenic |
| GGTGGTGATGGATACACAAT+TGG | 0.532639 | 1:-16113799 | None:intergenic |
| AAAACTCGCGCGGCCGCCGG+GGG | 0.535177 | 1:-16114016 | None:intergenic |
| CCAGTTGATGTGAATTTCAG+TGG | 0.543501 | 1:+16113982 | MsG0180001110.01.T01:CDS |
| TTTAATTTGAGGATCCATAA+CGG | 0.547937 | 1:-16113620 | None:intergenic |
| GATGATGATGATGGTGATGA+TGG | 0.548663 | 1:-16113916 | None:intergenic |
| GGTGAATAGTATACTATTGG+TGG | 0.549009 | 1:-16113844 | None:intergenic |
| ACTATTGGTGGTGGTGGCGA+CGG | 0.552115 | 1:-16113832 | None:intergenic |
| GAATAGTATACTATTGGTGG+TGG | 0.555298 | 1:-16113841 | None:intergenic |
| TCACCATATGTATACATGAC+TGG | 0.562728 | 1:+16113940 | MsG0180001110.01.T01:CDS |
| GAATTGTATATAATTGGAGG+TGG | 0.563198 | 1:-16113766 | None:intergenic |
| GGCGGTTTCTTAGGTGAAGG+TGG | 0.564632 | 1:-16113871 | None:intergenic |
| TAGTATACTATTGGTGGTGG+TGG | 0.565872 | 1:-16113838 | None:intergenic |
| AGTGGATCAGGAGCAACCCC+CGG | 0.566954 | 1:+16114000 | MsG0180001110.01.T01:CDS |
| GGAGGACAATTCGCCGAGGG+CGG | 0.571434 | 1:-16113889 | None:intergenic |
| GAGGGCGGTTTCTTAGGTGA+AGG | 0.572693 | 1:-16113874 | None:intergenic |
| GATGATGGTGATGATGGTGG+TGG | 0.578357 | 1:-16113910 | None:intergenic |
| TCAACTGGATATAGGTTCCC+CGG | 0.578812 | 1:-16113967 | None:intergenic |
| GGTTTCTTAGGTGAAGGTGG+TGG | 0.579798 | 1:-16113868 | None:intergenic |
| ATACACAATTGGTGGTGGTG+GGG | 0.580663 | 1:-16113788 | None:intergenic |
| GGGGAATTGTATATAATTGG+AGG | 0.581500 | 1:-16113769 | None:intergenic |
| GGTGGAGGACAATTCGCCGA+GGG | 0.591736 | 1:-16113892 | None:intergenic |
| GGATCCATAACGGTTCTGAT+GGG | 0.612417 | 1:-16113610 | None:intergenic |
| GATGGATACACAATTGGTGG+TGG | 0.614845 | 1:-16113793 | None:intergenic |
| GGTGATGGATACACAATTGG+TGG | 0.618689 | 1:-16113796 | None:intergenic |
| AATCTCAAACTAATCACCAA+AGG | 0.619142 | 1:-16114083 | None:intergenic |
| GATGATGATGGTGATGATGG+TGG | 0.629814 | 1:-16113913 | None:intergenic |
| TTCACCTAAGAAACCGCCCT+CGG | 0.637750 | 1:+16113876 | MsG0180001110.01.T01:CDS |
| AGAATCCAGCAAAACTCGCG+CGG | 0.642820 | 1:-16114026 | None:intergenic |
| CAAGTAATAAGATCCAATTG+AGG | 0.688613 | 1:-16113657 | None:intergenic |
| TGGTGGAGGACAATTCGCCG+AGG | 0.702415 | 1:-16113893 | None:intergenic |
| TATATTGGAGTGTCACCACA+TGG | 0.717065 | 1:-16113739 | None:intergenic |
| TATACATGACTGGACCGCCG+GGG | 0.732419 | 1:+16113950 | MsG0180001110.01.T01:CDS |
| ACTGGATATAGGTTCCCCGG+CGG | 0.797230 | 1:-16113964 | None:intergenic |
| GTTGAGAAGTGTACACCATG+TGG | 0.801579 | 1:+16113724 | MsG0180001110.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTAGTGGAATTTTAATTTG+AGG | - | Chr1:16113634-16113653 | None:intergenic | 25.0% |
| !!! | TTTAATTTGAGGATCCATAA+CGG | - | Chr1:16113623-16113642 | None:intergenic | 25.0% |
| AATTCACATCAACTGGATAT+AGG | - | Chr1:16113978-16113997 | None:intergenic | 30.0% | |
| CAAGTAATAAGATCCAATTG+AGG | - | Chr1:16113660-16113679 | None:intergenic | 30.0% | |
| CTCAATAAACAAACCAAAAG+TGG | - | Chr1:16114054-16114073 | None:intergenic | 30.0% | |
| CTTGATTCCATAGCATTAAT+TGG | - | Chr1:16113697-16113716 | None:intergenic | 30.0% | |
| GAATTGTATATAATTGGAGG+TGG | - | Chr1:16113769-16113788 | None:intergenic | 30.0% | |
| TACTCTTCCAATTAATGCTA+TGG | + | Chr1:16113687-16113706 | MsG0180001110.01.T01:CDS | 30.0% | |
| ATCCAATTGAGGATTCTTAG+TGG | - | Chr1:16113649-16113668 | None:intergenic | 35.0% | |
| ATTGGAGGTGGTGAATATAT+TGG | - | Chr1:16113757-16113776 | None:intergenic | 35.0% | |
| GAATAGTATACTATTGGTGG+TGG | - | Chr1:16113844-16113863 | None:intergenic | 35.0% | |
| GGGGAATTGTATATAATTGG+AGG | - | Chr1:16113772-16113791 | None:intergenic | 35.0% | |
| GGTGAATAGTATACTATTGG+TGG | - | Chr1:16113847-16113866 | None:intergenic | 35.0% | |
| GGTGGGGAATTGTATATAAT+TGG | - | Chr1:16113775-16113794 | None:intergenic | 35.0% | |
| GGTGGTGAATAGTATACTAT+TGG | - | Chr1:16113850-16113869 | None:intergenic | 35.0% | |
| TCACCATATGTATACATGAC+TGG | + | Chr1:16113940-16113959 | MsG0180001110.01.T01:CDS | 35.0% | |
| TTCCACTAAGAATCCTCAAT+TGG | + | Chr1:16113644-16113663 | MsG0180001110.01.T01:CDS | 35.0% | |
| ! | TATGGTGATGATGATGATGA+TGG | - | Chr1:16113928-16113947 | None:intergenic | 35.0% |
| AATTCCCATCAGAACCGTTA+TGG | + | Chr1:16113606-16113625 | MsG0180001110.01.T01:CDS | 40.0% | |
| CCACTGAAATTCACATCAAC+TGG | - | Chr1:16113985-16114004 | None:intergenic | 40.0% | |
| GATGTGAATTTCAGTGGATC+AGG | + | Chr1:16113988-16114007 | MsG0180001110.01.T01:CDS | 40.0% | |
| GGTCCAGTCATGTATACATA+TGG | - | Chr1:16113946-16113965 | None:intergenic | 40.0% | |
| TATTGAGCCTTCTAGTCCTT+TGG | + | Chr1:16114067-16114086 | MsG0180001110.01.T01:CDS | 40.0% | |
| ! | CCAGTTGATGTGAATTTCAG+TGG | + | Chr1:16113982-16114001 | MsG0180001110.01.T01:CDS | 40.0% |
| ! | TATATTGGAGTGTCACCACA+TGG | - | Chr1:16113742-16113761 | None:intergenic | 40.0% |
| !! | GATGATGATGATGGTGATGA+TGG | - | Chr1:16113919-16113938 | None:intergenic | 40.0% |
| !! | TAGTATACTATTGGTGGTGG+TGG | - | Chr1:16113841-16113860 | None:intergenic | 40.0% |
| GATGGATACACAATTGGTGG+TGG | - | Chr1:16113796-16113815 | None:intergenic | 45.0% | |
| TCAACTGGATATAGGTTCCC+CGG | - | Chr1:16113970-16113989 | None:intergenic | 45.0% | |
| ! | AGGATCCATAACGGTTCTGA+TGG | - | Chr1:16113614-16113633 | None:intergenic | 45.0% |
| ! | GGATCCATAACGGTTCTGAT+GGG | - | Chr1:16113613-16113632 | None:intergenic | 45.0% |
| ! | GTTGAGAAGTGTACACCATG+TGG | + | Chr1:16113724-16113743 | MsG0180001110.01.T01:CDS | 45.0% |
| !! | ATACACAATTGGTGGTGGTG+GGG | - | Chr1:16113791-16113810 | None:intergenic | 45.0% |
| !! | GATACACAATTGGTGGTGGT+GGG | - | Chr1:16113792-16113811 | None:intergenic | 45.0% |
| !! | GATGATGATGGTGATGATGG+TGG | - | Chr1:16113916-16113935 | None:intergenic | 45.0% |
| !! | GGTGATGGATACACAATTGG+TGG | - | Chr1:16113799-16113818 | None:intergenic | 45.0% |
| !! | GGTGGTGATGGATACACAAT+TGG | - | Chr1:16113802-16113821 | None:intergenic | 45.0% |
| !!! | TGCTGGATTCTTGCCACTTT+TGG | + | Chr1:16114038-16114057 | MsG0180001110.01.T01:CDS | 45.0% |
| AGAATCCAGCAAAACTCGCG+CGG | - | Chr1:16114029-16114048 | None:intergenic | 50.0% | |
| GGTTTCTTAGGTGAAGGTGG+TGG | - | Chr1:16113871-16113890 | None:intergenic | 50.0% | |
| TGTATACATGACTGGACCGC+CGG | + | Chr1:16113948-16113967 | MsG0180001110.01.T01:CDS | 50.0% | |
| TTCACCTAAGAAACCGCCCT+CGG | + | Chr1:16113876-16113895 | MsG0180001110.01.T01:CDS | 50.0% | |
| ! | TTCTTAGGTGAAGGTGGTGG+TGG | - | Chr1:16113868-16113887 | None:intergenic | 50.0% |
| !! | GATGATGGTGATGATGGTGG+TGG | - | Chr1:16113913-16113932 | None:intergenic | 50.0% |
| !! | GGATACACAATTGGTGGTGG+TGG | - | Chr1:16113793-16113812 | None:intergenic | 50.0% |
| ACTGGATATAGGTTCCCCGG+CGG | - | Chr1:16113967-16113986 | None:intergenic | 55.0% | |
| GAGGGCGGTTTCTTAGGTGA+AGG | - | Chr1:16113877-16113896 | None:intergenic | 55.0% | |
| GGCGGTTTCTTAGGTGAAGG+TGG | - | Chr1:16113874-16113893 | None:intergenic | 55.0% | |
| GTATACATGACTGGACCGCC+GGG | + | Chr1:16113949-16113968 | MsG0180001110.01.T01:CDS | 55.0% | |
| TATACATGACTGGACCGCCG+GGG | + | Chr1:16113950-16113969 | MsG0180001110.01.T01:CDS | 55.0% | |
| !! | ACTATTGGTGGTGGTGGCGA+CGG | - | Chr1:16113835-16113854 | None:intergenic | 55.0% |
| !! | GATGGTGATGATGGTGGTGG+AGG | - | Chr1:16113910-16113929 | None:intergenic | 55.0% |
| AGTGGATCAGGAGCAACCCC+CGG | + | Chr1:16114000-16114019 | MsG0180001110.01.T01:CDS | 60.0% | |
| GGTGGAGGACAATTCGCCGA+GGG | - | Chr1:16113895-16113914 | None:intergenic | 60.0% | |
| TGGTGGAGGACAATTCGCCG+AGG | - | Chr1:16113896-16113915 | None:intergenic | 60.0% | |
| TTCGCCGAGGGCGGTTTCTT+AGG | - | Chr1:16113883-16113902 | None:intergenic | 60.0% | |
| !! | GGAGATGGTGGAGGTGGTGA+TGG | - | Chr1:16113814-16113833 | None:intergenic | 60.0% |
| GGAGGACAATTCGCCGAGGG+CGG | - | Chr1:16113892-16113911 | None:intergenic | 65.0% | |
| AGCAAAACTCGCGCGGCCGC+CGG | - | Chr1:16114022-16114041 | None:intergenic | 70.0% | |
| GGATCAGGAGCAACCCCCGG+CGG | + | Chr1:16114003-16114022 | MsG0180001110.01.T01:CDS | 70.0% | |
| ! | GGTGGCGACGGAGATGGTGG+AGG | - | Chr1:16113823-16113842 | None:intergenic | 70.0% |
| ! | GGTGGTGGCGACGGAGATGG+TGG | - | Chr1:16113826-16113845 | None:intergenic | 70.0% |
| !! | GGCGACGGAGATGGTGGAGG+TGG | - | Chr1:16113820-16113839 | None:intergenic | 70.0% |
| !! | GGTGGTGGTGGCGACGGAGA+TGG | - | Chr1:16113829-16113848 | None:intergenic | 70.0% |
| AAAACTCGCGCGGCCGCCGG+GGG | - | Chr1:16114019-16114038 | None:intergenic | 75.0% | |
| CAAAACTCGCGCGGCCGCCG+GGG | - | Chr1:16114020-16114039 | None:intergenic | 75.0% | |
| GCAAAACTCGCGCGGCCGCC+GGG | - | Chr1:16114021-16114040 | None:intergenic | 75.0% | |
| ! | GGCGGCCGCGCGAGTTTTGC+TGG | + | Chr1:16114021-16114040 | MsG0180001110.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 16113592 | 16114092 | 16113592 | ID=MsG0180001110.01;Name=MsG0180001110.01 |
| Chr1 | mRNA | 16113592 | 16114092 | 16113592 | ID=MsG0180001110.01.T01;Parent=MsG0180001110.01;Name=MsG0180001110.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|166 |
| Chr1 | exon | 16113592 | 16114092 | 16113592 | ID=MsG0180001110.01.T01:exon:31200;Parent=MsG0180001110.01.T01 |
| Chr1 | CDS | 16113592 | 16114092 | 16113592 | ID=MsG0180001110.01.T01:cds;Parent=MsG0180001110.01.T01 |
| Gene Sequence |
| Protein sequence |
