AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024316.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024316.01.T01 MTR_5g008890 99.444 180 1 0 1 180 1 180 6.61e-132 367
MsG0580024316.01.T01 MTR_3g054060 48.276 174 52 5 1 171 31 169 8.65e-44 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024316.01.T01 AT1G51510 64.246 179 54 6 9 178 7 184 5.27e-74 221
MsG0580024316.01.T01 AT5G64200 32.353 102 68 1 79 180 5 105 5.29e-11 60.5
MsG0580024316.01.T01 AT5G64200 32.353 102 68 1 79 180 5 105 5.29e-11 60.5

Find 53 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAACCATTTATGTTGAT+TGG 0.221624 5:+3303049 MsG0580024316.01.T01:CDS
TATTGAAGGATGGATTATTT+TGG 0.283878 5:+3300652 MsG0580024316.01.T01:CDS
AACTCTCGTCTCGCTGATTC+CGG 0.283971 5:+3300494 MsG0580024316.01.T01:CDS
GAAAGCCCAATCAACATAAA+TGG 0.296555 5:-3303055 None:intergenic
CGCCGCCGACGGAGAAGCTT+TGG 0.306556 5:+3300316 MsG0580024316.01.T01:CDS
TAATTCAAACGAATTGGAAT+TGG 0.314736 5:+3300224 MsG0580024316.01.T01:five_prime_UTR
AGTGATGGCGGATTTGAGTT+TGG 0.318147 5:-3300411 None:intergenic
GATGATTTGCAGAATGCTTT+TGG 0.361063 5:+3300704 MsG0580024316.01.T01:CDS
GTGATGGCGGATTTGAGTTT+GGG 0.363430 5:-3300410 None:intergenic
CTGACGGAAACCGCGACCTT+TGG 0.364553 5:-3300450 None:intergenic
CTTCTAACTGACTCATTGAT+AGG 0.375297 5:-3303087 None:intergenic
ACAAACCATTTATGTTGATT+GGG 0.381801 5:+3303050 MsG0580024316.01.T01:CDS
TCGTTGTAATTCAAACGAAT+TGG 0.383790 5:+3300218 None:intergenic
AGAGAGGAGTGAGTGTATGA+AGG 0.395274 5:-3300271 None:intergenic
AATTCAAACGAATTGGAATT+GGG 0.407430 5:+3300225 MsG0580024316.01.T01:five_prime_UTR
CTCACTGCTGAGGGTGGTCC+TGG 0.420911 5:+3300527 MsG0580024316.01.T01:CDS
GCCAGAGGAAGATGACTTGA+TGG 0.429124 5:+3300346 MsG0580024316.01.T01:CDS
ATGAGTCAGTTAGAAGAAAC+AGG 0.431891 5:+3303094 MsG0580024316.01.T01:CDS
GAGACGAGAGTTACGGTTAG+AGG 0.454033 5:-3300483 None:intergenic
GCGGATTTGAGTTTGGGATG+TGG 0.456064 5:-3300404 None:intergenic
CAAAGGTCGCGGTTTCCGTC+AGG 0.458944 5:+3300451 MsG0580024316.01.T01:CDS
GAGAGGAGTGAGTGTATGAA+GGG 0.466217 5:-3300270 None:intergenic
TTGAATCTTGATCGCCGTAC+TGG 0.470728 5:+3300755 MsG0580024316.01.T01:CDS
AGAGTTACGGTTAGAGGAAT+CGG 0.477329 5:-3300477 None:intergenic
TCCATCAAGTCATCTTCCTC+TGG 0.480725 5:-3300347 None:intergenic
GTTGATTGGGCTTTCAGCAG+TGG 0.486700 5:+3303063 MsG0580024316.01.T01:CDS
AATGCAATAGAGAATTTGAA+TGG 0.500993 5:+3303012 MsG0580024316.01.T01:CDS
TGGGGATACGAACATCTTTG+AGG 0.513262 5:-3300551 None:intergenic
CTCTATTCAGCAACGATGCA+AGG 0.516755 5:+3300290 MsG0580024316.01.T01:exon
GGGATGTGGAGGAGAAGCTT+CGG 0.519462 5:-3300390 None:intergenic
AGCAGTGAGAGTATCGAAAC+CGG 0.528470 5:-3300513 None:intergenic
TTCGATACTCTCACTGCTGA+GGG 0.535196 5:+3300518 MsG0580024316.01.T01:CDS
GAATTGGAATTGGGAACCCT+AGG 0.543155 5:+3300234 MsG0580024316.01.T01:five_prime_UTR
ATGCAAGGAAGCGCCGCCGA+CGG 0.551395 5:+3300305 MsG0580024316.01.T01:CDS
GGGTGGTTCAATTCAACCTA+GGG 0.552325 5:-3300250 None:intergenic
AGGAGTGAGTGTATGAAGGG+TGG 0.557404 5:-3300267 None:intergenic
ATGAGCGCTCTGAGGAAGCT+CGG 0.562915 5:+3302989 MsG0580024316.01.T01:CDS
AATCAGCGAGACGAGAGTTA+CGG 0.563114 5:-3300490 None:intergenic
TACGAACATCTTTGAGGACC+AGG 0.563747 5:-3300545 None:intergenic
GATGACTTGATGGACGAAGA+AGG 0.572561 5:+3300356 MsG0580024316.01.T01:CDS
GATTTGAGTTTGGGATGTGG+AGG 0.573507 5:-3300401 None:intergenic
AGCTTTGGATTTCGAGCCAG+AGG 0.579650 5:+3300331 MsG0580024316.01.T01:CDS
ATCCAAAGCTTCTCCGTCGG+CGG 0.583085 5:-3300318 None:intergenic
AGGGTGGTTCAATTCAACCT+AGG 0.589115 5:-3300251 None:intergenic
TTTCGATACTCTCACTGCTG+AGG 0.590321 5:+3300517 MsG0580024316.01.T01:CDS
TTTGGGAACGGAAGTGATGG+CGG 0.614347 5:-3300423 None:intergenic
GATACTCTCACTGCTGAGGG+TGG 0.647746 5:+3300521 MsG0580024316.01.T01:CDS
TGCATCGTTGCTGAATAGAG+AGG 0.657759 5:-3300287 None:intergenic
GCACCTTAACAAAACCAGTA+CGG 0.658816 5:-3300769 None:intergenic
TTTGGTGACTGGTGTACATG+AGG 0.661358 5:+3300670 MsG0580024316.01.T01:CDS
TAGAGGAATCGGAATCCTGA+CGG 0.677725 5:-3300466 None:intergenic
GATTGAATATGAGCGCTCTG+AGG 0.688874 5:+3302981 MsG0580024316.01.T01:CDS
GAAATCCAAAGCTTCTCCGT+CGG 0.701772 5:-3300321 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCTTAATAAAATAAGATA+AGG - Chr5:3302702-3302721 None:intergenic 10.0%
!!! TATCTTATTTTATTAAGAAA+AGG + Chr5:3302702-3302721 MsG0580024316.01.T01:intron 10.0%
!! AAATTGACAAAAATTGAAAT+TGG - Chr5:3300574-3300593 None:intergenic 15.0%
!! AATTGACAAAAATTGAAATT+GGG - Chr5:3300573-3300592 None:intergenic 15.0%
!!! TACTTTTCATTCTTAAATTA+TGG + Chr5:3301014-3301033 MsG0580024316.01.T01:intron 15.0%
!! ATTGACAAAAATTGAAATTG+GGG - Chr5:3300572-3300591 None:intergenic 20.0%
!! ATTTGAAAAAAAAATACTGC+AGG - Chr5:3302501-3302520 None:intergenic 20.0%
!! TTGAGAAATATATGGAAAAA+TGG - Chr5:3301683-3301702 None:intergenic 20.0%
!! TTTATAGACAGTGTATATTA+CGG - Chr5:3301873-3301892 None:intergenic 20.0%
!! TTTGAAAAAAAAATACTGCA+GGG - Chr5:3302500-3302519 None:intergenic 20.0%
!! TTTGGAAGAAGAAAAAAAAA+AGG - Chr5:3301381-3301400 None:intergenic 20.0%
!!! AATGCAGTTTTGATTTTTAA+AGG + Chr5:3302546-3302565 MsG0580024316.01.T01:intron 20.0%
!!! CTTTGAAAAAAAAAAAAGGA+AGG + Chr5:3301479-3301498 MsG0580024316.01.T01:intron 20.0%
!!! TACTTGTAATTTTGTAAACA+GGG + Chr5:3302745-3302764 MsG0580024316.01.T01:intron 20.0%
!!! TGATAAGCATTTTGATTTTA+GGG + Chr5:3302949-3302968 MsG0580024316.01.T01:intron 20.0%
! AAAACAAAACCCTCATAATT+TGG - Chr5:3301291-3301310 None:intergenic 25.0%
! AAACAAAACCCTCATAATTT+GGG - Chr5:3301290-3301309 None:intergenic 25.0%
! AAGTTGCTAAATAAAGTTAC+AGG - Chr5:3302877-3302896 None:intergenic 25.0%
! AATGCAATAGAGAATTTGAA+TGG + Chr5:3303012-3303031 MsG0580024316.01.T01:CDS 25.0%
! AATTCAAACGAATTGGAATT+GGG + Chr5:3300225-3300244 MsG0580024316.01.T01:five_prime_UTR 25.0%
! AATTCAAGTCATGAATCAAA+TGG + Chr5:3301967-3301986 MsG0580024316.01.T01:intron 25.0%
! AGTGTATTAATCTATGTTGT+TGG - Chr5:3301164-3301183 None:intergenic 25.0%
! CAAATCTTCACATGATAAAA+TGG - Chr5:3302919-3302938 None:intergenic 25.0%
! CAGAAGGTTAACAATAAAAA+AGG - Chr5:3301758-3301777 None:intergenic 25.0%
! CTAAATAAAGTTACAGGAAA+AGG - Chr5:3302871-3302890 None:intergenic 25.0%
! GAAGAAGAAAAAAAAAAGGA+AGG - Chr5:3301377-3301396 None:intergenic 25.0%
! GTGTATCATTGTATCTATAT+GGG - Chr5:3302113-3302132 None:intergenic 25.0%
! GTGTATTAATCTATGTTGTT+GGG - Chr5:3301163-3301182 None:intergenic 25.0%
! TAATTCAAACGAATTGGAAT+TGG + Chr5:3300224-3300243 MsG0580024316.01.T01:five_prime_UTR 25.0%
! TCATGACTTGAATTATTTGA+AGG - Chr5:3301961-3301980 None:intergenic 25.0%
! TGTGTATCATTGTATCTATA+TGG - Chr5:3302114-3302133 None:intergenic 25.0%
! TTACCATTAATCAATCACTT+AGG - Chr5:3301720-3301739 None:intergenic 25.0%
!! AAAATTTGATAACCATGATC+CGG - Chr5:3301809-3301828 None:intergenic 25.0%
!! ACAAACCATTTATGTTGATT+GGG + Chr5:3303050-3303069 MsG0580024316.01.T01:CDS 25.0%
!! CATTTTATCATGTGAAGATT+TGG + Chr5:3302917-3302936 MsG0580024316.01.T01:intron 25.0%
!! CTACTTGTAATTTTGTAAAC+AGG + Chr5:3302744-3302763 MsG0580024316.01.T01:intron 25.0%
!! TGAATCAGAAGATCTATTTT+CGG + Chr5:3301828-3301847 MsG0580024316.01.T01:intron 25.0%
!! TTTGAAGTAGGAAATGTATA+TGG + Chr5:3300930-3300949 MsG0580024316.01.T01:intron 25.0%
!!! AATTGCGTGATTTTGATTTT+AGG + Chr5:3300594-3300613 MsG0580024316.01.T01:intron 25.0%
!!! AGGGCTTTGAAAAAAAAAAA+AGG + Chr5:3301475-3301494 MsG0580024316.01.T01:intron 25.0%
!!! ATTGCGTGATTTTGATTTTA+GGG + Chr5:3300595-3300614 MsG0580024316.01.T01:intron 25.0%
!!! CTGATAAGCATTTTGATTTT+AGG + Chr5:3302948-3302967 MsG0580024316.01.T01:intron 25.0%
!!! CTTTTTTATTGTTAACCTTC+TGG + Chr5:3301756-3301775 MsG0580024316.01.T01:intron 25.0%
!!! GTATAGATTGACTAGTTTTA+TGG + Chr5:3300872-3300891 MsG0580024316.01.T01:intron 25.0%
!!! TATTGAAGGATGGATTATTT+TGG + Chr5:3300652-3300671 MsG0580024316.01.T01:CDS 25.0%
!!! TTGATTTTTAAAGGATGTAG+TGG + Chr5:3302555-3302574 MsG0580024316.01.T01:intron 25.0%
AACAAAACCCTCATAATTTG+GGG - Chr5:3301289-3301308 None:intergenic 30.0%
AAGCCTAAGTGATTGATTAA+TGG + Chr5:3301714-3301733 MsG0580024316.01.T01:intron 30.0%
AAGTAGGAAATGTATATGGA+CGG + Chr5:3300934-3300953 MsG0580024316.01.T01:intron 30.0%
ATGAGATGATAACTCAGTTA+AGG - Chr5:3302305-3302324 None:intergenic 30.0%
ATTAATCTATGTTGTTGGGA+GGG - Chr5:3301159-3301178 None:intergenic 30.0%
CACAAACCATTTATGTTGAT+TGG + Chr5:3303049-3303068 MsG0580024316.01.T01:CDS 30.0%
CGATTCTCATTTCATTCTAA+TGG - Chr5:3302057-3302076 None:intergenic 30.0%
TATGAACATAGAAACAGAGA+TGG - Chr5:3302798-3302817 None:intergenic 30.0%
TATTAATCTATGTTGTTGGG+AGG - Chr5:3301160-3301179 None:intergenic 30.0%
TGGATGAAATAGAAGACATT+GGG + Chr5:3301508-3301527 MsG0580024316.01.T01:intron 30.0%
TTGGGAGTTTGAGAAATATA+TGG - Chr5:3301691-3301710 None:intergenic 30.0%
! AATCTAAGGGTATGATTGTA+GGG + Chr5:3300965-3300984 MsG0580024316.01.T01:intron 30.0%
! TAATCTAAGGGTATGATTGT+AGG + Chr5:3300964-3300983 MsG0580024316.01.T01:intron 30.0%
! TTTTTGTAGCTATTGAAGGA+TGG + Chr5:3300642-3300661 MsG0580024316.01.T01:intron 30.0%
!! GAGAATCGTTTTAGTGAATT+GGG + Chr5:3302069-3302088 MsG0580024316.01.T01:intron 30.0%
!! TGAGAATCGTTTTAGTGAAT+TGG + Chr5:3302068-3302087 MsG0580024316.01.T01:intron 30.0%
!!! GATAGAAAGTGTTTTGAAGT+AGG + Chr5:3300918-3300937 MsG0580024316.01.T01:intron 30.0%
AAAAAAAAGGAAGGAGCAAG+TGG + Chr5:3301488-3301507 MsG0580024316.01.T01:intron 35.0%
AAAAAAAAGGAAGGAGCAAG+TGG - Chr5:3301507-3301488 None:intergenic 35.0%
AAACTCCACATCATGGTTAT+CGG - Chr5:3302206-3302225 None:intergenic 35.0%
AAATGTATATGGACGGGTAA+TGG + Chr5:3300941-3300960 MsG0580024316.01.T01:intron 35.0%
AACTAGTCAATCTATACGAG+TGG - Chr5:3300869-3300888 None:intergenic 35.0%
AATACAAAATCCCCCTCATT+TGG + Chr5:3301564-3301583 MsG0580024316.01.T01:intron 35.0%
ACAAAACCCTCATAATTTGG+GGG - Chr5:3301288-3301307 None:intergenic 35.0%
ACAATGATACACACTAACTG+AGG + Chr5:3302121-3302140 MsG0580024316.01.T01:intron 35.0%
ACACTAACTGAGGTTATTGA+TGG + Chr5:3302131-3302150 MsG0580024316.01.T01:intron 35.0%
ACTAGTCAATCTATACGAGT+GGG - Chr5:3300868-3300887 None:intergenic 35.0%
AGTAGGAAATGTATATGGAC+GGG + Chr5:3300935-3300954 MsG0580024316.01.T01:intron 35.0%
AGTGAATTGGGATTCAAGAT+GGG + Chr5:3302081-3302100 MsG0580024316.01.T01:intron 35.0%
ATACAAAATCCCCCTCATTT+GGG + Chr5:3301565-3301584 MsG0580024316.01.T01:intron 35.0%
ATGAAATAGAAGACATTGGG+AGG + Chr5:3301511-3301530 MsG0580024316.01.T01:intron 35.0%
ATGAGTCAGTTAGAAGAAAC+AGG + Chr5:3303094-3303113 MsG0580024316.01.T01:CDS 35.0%
CACTAACTGAGGTTATTGAT+GGG + Chr5:3302132-3302151 MsG0580024316.01.T01:intron 35.0%
GAAAGCCCAATCAACATAAA+TGG - Chr5:3303058-3303077 None:intergenic 35.0%
GAGATGGCAGAAAATTTACT+TGG - Chr5:3302782-3302801 None:intergenic 35.0%
GGAAAGTTAAGTCCAACTAA+CGG - Chr5:3302337-3302356 None:intergenic 35.0%
GGAACTCAAAATTGTATTCG+AGG - Chr5:3301266-3301285 None:intergenic 35.0%
GTGGATGAAATAGAAGACAT+TGG + Chr5:3301507-3301526 MsG0580024316.01.T01:intron 35.0%
TAGTGAATTGGGATTCAAGA+TGG + Chr5:3302080-3302099 MsG0580024316.01.T01:intron 35.0%
TGAAATAGAAGACATTGGGA+GGG + Chr5:3301512-3301531 MsG0580024316.01.T01:intron 35.0%
TTAATCTATGTTGTTGGGAG+GGG - Chr5:3301158-3301177 None:intergenic 35.0%
! AATATCAAGGTACCTTTCCT+CGG - Chr5:3301786-3301805 None:intergenic 35.0%
! CAGAATGCTTTTGGTGAATA+TGG + Chr5:3300713-3300732 MsG0580024316.01.T01:CDS 35.0%
! CATATCTTTTCACCGTTAGT+TGG + Chr5:3302322-3302341 MsG0580024316.01.T01:intron 35.0%
! CTAAGGCTTTGTCTTTGTTT+GGG - Chr5:3301426-3301445 None:intergenic 35.0%
! CTTCTAACTGACTCATTGAT+AGG - Chr5:3303090-3303109 None:intergenic 35.0%
! GAATTATAGCCAGCTTTTGT+CGG - Chr5:3302015-3302034 None:intergenic 35.0%
! TCTTTGTTTGGGAGTTTAGA+GGG - Chr5:3301415-3301434 None:intergenic 35.0%
!! CAATCACTTAGGCTTTGTTT+GGG - Chr5:3301709-3301728 None:intergenic 35.0%
!! GATGATTTGCAGAATGCTTT+TGG + Chr5:3300704-3300723 MsG0580024316.01.T01:CDS 35.0%
!! TCAATCACTTAGGCTTTGTT+TGG - Chr5:3301710-3301729 None:intergenic 35.0%
!!! ACCTTTGGTTTTTTTGGGAA+CGG - Chr5:3300438-3300457 None:intergenic 35.0%
!!! GCTGTTTTTGTAGCTATTGA+AGG + Chr5:3300638-3300657 MsG0580024316.01.T01:intron 35.0%
!!! TATGATTGTAGGGTTTTGGA+GGG + Chr5:3300975-3300994 MsG0580024316.01.T01:intron 35.0%
!!! TATTCGAGGAGAGTTTTGAA+GGG - Chr5:3301252-3301271 None:intergenic 35.0%
AAACTCCCAAACAAAGCCTA+AGG - Chr5:3301720-3301701 None:intergenic 40.0%
AACCATGATCCGGAATATCA+AGG - Chr5:3301799-3301818 None:intergenic 40.0%
AGAGTTACGGTTAGAGGAAT+CGG - Chr5:3300480-3300499 None:intergenic 40.0%
AGATACCGATAACCATGATG+TGG + Chr5:3302198-3302217 MsG0580024316.01.T01:intron 40.0%
AGGAAATAGAGGAGTTTGAG+AGG - Chr5:3301345-3301364 None:intergenic 40.0%
ATTGAGAGGTAGGTAAAGTG+AGG + Chr5:3302227-3302246 MsG0580024316.01.T01:intron 40.0%
CAAAACCCTCATAATTTGGG+GGG - Chr5:3301287-3301306 None:intergenic 40.0%
CAGTTAAGGTTGCATCAATC+AGG - Chr5:3302291-3302310 None:intergenic 40.0%
CAGTTTCAGAAACTGCAGAT+TGG + Chr5:3302841-3302860 MsG0580024316.01.T01:intron 40.0%
CCAAACAAAGACAAAGCCTT+AGG + Chr5:3301424-3301443 MsG0580024316.01.T01:intron 40.0%
CCTAACAAGTAACTGTGTGT+AGG + Chr5:3302596-3302615 MsG0580024316.01.T01:intron 40.0%
CCTACACACAGTTACTTGTT+AGG - Chr5:3302599-3302618 None:intergenic 40.0%
CTCAATCAAACTCCACATCA+TGG - Chr5:3302213-3302232 None:intergenic 40.0%
GAAATAGAAGACATTGGGAG+GGG + Chr5:3301513-3301532 MsG0580024316.01.T01:intron 40.0%
GAAATAGAGGAGTTTGAGAG+GGG - Chr5:3301343-3301362 None:intergenic 40.0%
GCACCTTAACAAAACCAGTA+CGG - Chr5:3300772-3300791 None:intergenic 40.0%
GGAAATAGAGGAGTTTGAGA+GGG - Chr5:3301344-3301363 None:intergenic 40.0%
TACAAAATCCCCCTCATTTG+GGG + Chr5:3301566-3301585 MsG0580024316.01.T01:intron 40.0%
TACCTTGATATTCCGGATCA+TGG + Chr5:3301794-3301813 MsG0580024316.01.T01:intron 40.0%
TCATGAATCAAATGGCAAGC+TGG + Chr5:3301975-3301994 MsG0580024316.01.T01:intron 40.0%
TCCATTTCATGTCTCAGTCA+TGG + Chr5:3302354-3302373 MsG0580024316.01.T01:intron 40.0%
TCCGTTCCCAAAAAAACCAA+AGG + Chr5:3300434-3300453 MsG0580024316.01.T01:CDS 40.0%
TGATGTGGAGTTTGATTGAG+AGG + Chr5:3302213-3302232 MsG0580024316.01.T01:intron 40.0%
! AGGGTATGATTGTAGGGTTT+TGG + Chr5:3300971-3300990 MsG0580024316.01.T01:intron 40.0%
! ATATCAAGGTACCTTTCCTC+GGG - Chr5:3301785-3301804 None:intergenic 40.0%
! CACACAGTTACTTGTTAGGA+AGG - Chr5:3302595-3302614 None:intergenic 40.0%
! CCTAAGGCTTTGTCTTTGTT+TGG - Chr5:3301427-3301446 None:intergenic 40.0%
! GACGGGTAATGGTAATCTAA+GGG + Chr5:3300952-3300971 MsG0580024316.01.T01:intron 40.0%
! GTCTTTGTTTGGGAGTTTAG+AGG - Chr5:3301416-3301435 None:intergenic 40.0%
! TTGACCTCAAGCTGTTACTT+TGG + Chr5:3302410-3302429 MsG0580024316.01.T01:intron 40.0%
!! ACAAAGCCTTAGGCTTTGTT+TGG + Chr5:3301434-3301453 MsG0580024316.01.T01:intron 40.0%
!! CAAAGCCTTAGGCTTTGTTT+GGG + Chr5:3301435-3301454 MsG0580024316.01.T01:intron 40.0%
!! GCTTTGTTTGGGAGTTTAGA+GGG + Chr5:3301446-3301465 MsG0580024316.01.T01:intron 40.0%
!! GGAAAGGTACCTTGATATTC+CGG + Chr5:3301787-3301806 MsG0580024316.01.T01:intron 40.0%
!! GTATGATTGTAGGGTTTTGG+AGG + Chr5:3300974-3300993 MsG0580024316.01.T01:intron 40.0%
!! GTATTCGAGGAGAGTTTTGA+AGG - Chr5:3301253-3301272 None:intergenic 40.0%
!! TGACCTCAAGCTGTTACTTT+GGG + Chr5:3302411-3302430 MsG0580024316.01.T01:intron 40.0%
!!! AGGAGAGTTTTGAAGGGATT+TGG - Chr5:3301246-3301265 None:intergenic 40.0%
!!! ATGATTGTAGGGTTTTGGAG+GGG + Chr5:3300976-3300995 MsG0580024316.01.T01:intron 40.0%
!!! GAGTTTTGAAGGGATTTGGA+AGG - Chr5:3301242-3301261 None:intergenic 40.0%
!!! GGATGGATTATTTTGGTGAC+TGG + Chr5:3300659-3300678 MsG0580024316.01.T01:CDS 40.0%
!!! TTTTGAGTTCCCCCAAATGA+GGG - Chr5:3301579-3301598 None:intergenic 40.0%
!!! TTTTTGAGTTCCCCCAAATG+AGG - Chr5:3301580-3301599 None:intergenic 40.0%
!!! TTTTTTGGGAACGGAAGTGA+TGG - Chr5:3300429-3300448 None:intergenic 40.0%
AAAAAGGAAGGAGCAAGTGG+AGG - Chr5:3301510-3301491 None:intergenic 45.0%
AACCCCAAAGTAACAGCTTG+AGG - Chr5:3302417-3302436 None:intergenic 45.0%
AATCAGCGAGACGAGAGTTA+CGG - Chr5:3300493-3300512 None:intergenic 45.0%
ACAAAATCCCCCTCATTTGG+GGG + Chr5:3301567-3301586 MsG0580024316.01.T01:intron 45.0%
AGAGAGGAGTGAGTGTATGA+AGG - Chr5:3300274-3300293 None:intergenic 45.0%
AGGGTGGTTCAATTCAACCT+AGG - Chr5:3300254-3300273 None:intergenic 45.0%
CAATGTTGACCGACAAAAGC+TGG + Chr5:3302003-3302022 MsG0580024316.01.T01:intron 45.0%
CCCAAAAAAACCAAAGGTCG+CGG + Chr5:3300440-3300459 MsG0580024316.01.T01:CDS 45.0%
CTCTATTCAGCAACGATGCA+AGG + Chr5:3300290-3300309 MsG0580024316.01.T01:exon 45.0%
GAAATCCAAAGCTTCTCCGT+CGG - Chr5:3300324-3300343 None:intergenic 45.0%
GAATTGGAATTGGGAACCCT+AGG + Chr5:3300234-3300253 MsG0580024316.01.T01:five_prime_UTR 45.0%
GAGAGGAGTGAGTGTATGAA+GGG - Chr5:3300273-3300292 None:intergenic 45.0%
GAGTTCCCCCCAAATTATGA+GGG + Chr5:3301279-3301298 MsG0580024316.01.T01:intron 45.0%
GATTGAATATGAGCGCTCTG+AGG + Chr5:3302981-3303000 MsG0580024316.01.T01:CDS 45.0%
GCCATGACTGAGACATGAAA+TGG - Chr5:3302358-3302377 None:intergenic 45.0%
GGAGTTTAGAGGGAAGTGAA+GGG + Chr5:3301456-3301475 MsG0580024316.01.T01:intron 45.0%
GGGTGGTTCAATTCAACCTA+GGG - Chr5:3300253-3300272 None:intergenic 45.0%
GTGGAGTTTGATTGAGAGGT+AGG + Chr5:3302217-3302236 MsG0580024316.01.T01:intron 45.0%
TAGAGGAATCGGAATCCTGA+CGG - Chr5:3300469-3300488 None:intergenic 45.0%
TCCAGCATGGGATCTAACTT+GGG + Chr5:3302261-3302280 MsG0580024316.01.T01:intron 45.0%
TCCATCAAGTCATCTTCCTC+TGG - Chr5:3300350-3300369 None:intergenic 45.0%
TCCCATGCTGGAACAATCTT+CGG - Chr5:3302253-3302272 None:intergenic 45.0%
TGAGATCAAAGCAGCGAAAG+TGG - Chr5:3302679-3302698 None:intergenic 45.0%
TGAGTTCCCCCCAAATTATG+AGG + Chr5:3301278-3301297 MsG0580024316.01.T01:intron 45.0%
TGCATCGTTGCTGAATAGAG+AGG - Chr5:3300290-3300309 None:intergenic 45.0%
TGGGGATACGAACATCTTTG+AGG - Chr5:3300554-3300573 None:intergenic 45.0%
TTCCAGCATGGGATCTAACT+TGG + Chr5:3302260-3302279 MsG0580024316.01.T01:intron 45.0%
TTCGATACTCTCACTGCTGA+GGG + Chr5:3300518-3300537 MsG0580024316.01.T01:CDS 45.0%
TTGAATCTTGATCGCCGTAC+TGG + Chr5:3300755-3300774 MsG0580024316.01.T01:CDS 45.0%
TTTCGATACTCTCACTGCTG+AGG + Chr5:3300517-3300536 MsG0580024316.01.T01:CDS 45.0%
TTTGGGAGTTTAGAGGGCAA+GGG - Chr5:3301409-3301428 None:intergenic 45.0%
! GATGACTTGATGGACGAAGA+AGG + Chr5:3300356-3300375 MsG0580024316.01.T01:CDS 45.0%
! GATTTGAGTTTGGGATGTGG+AGG - Chr5:3300404-3300423 None:intergenic 45.0%
! GGACGGGTAATGGTAATCTA+AGG + Chr5:3300951-3300970 MsG0580024316.01.T01:intron 45.0%
! TACGAACATCTTTGAGGACC+AGG - Chr5:3300548-3300567 None:intergenic 45.0%
! TATGTTGTTGGGAGGGGTTT+TGG - Chr5:3301152-3301171 None:intergenic 45.0%
! TTTGAGTTCCCCCAAATGAG+GGG - Chr5:3301578-3301597 None:intergenic 45.0%
! TTTGGTGACTGGTGTACATG+AGG + Chr5:3300670-3300689 MsG0580024316.01.T01:CDS 45.0%
!! AGCAGTGAGAGTATCGAAAC+CGG - Chr5:3300516-3300535 None:intergenic 45.0%
!! AGTGATGGCGGATTTGAGTT+TGG - Chr5:3300414-3300433 None:intergenic 45.0%
!! CTGTTACTTTGGGGTTCAGA+TGG + Chr5:3302421-3302440 MsG0580024316.01.T01:intron 45.0%
!! GACCTCAAGCTGTTACTTTG+GGG + Chr5:3302412-3302431 MsG0580024316.01.T01:intron 45.0%
!! GGCTTTGTTTGGGAGTTTAG+AGG + Chr5:3301445-3301464 MsG0580024316.01.T01:intron 45.0%
!! GTGATGGCGGATTTGAGTTT+GGG - Chr5:3300413-3300432 None:intergenic 45.0%
!!! ACCGCGACCTTTGGTTTTTT+TGG - Chr5:3300444-3300463 None:intergenic 45.0%
!!! CCGCGACCTTTGGTTTTTTT+GGG - Chr5:3300443-3300462 None:intergenic 45.0%
!!! TCGCCGTACTGGTTTTGTTA+AGG + Chr5:3300766-3300785 MsG0580024316.01.T01:CDS 45.0%
!!! TGTATATAAATTTTATAAAA+TGG + Chr5:3302462-3302481 MsG0580024316.01.T01:intron 5.0%
ACCCAAGTTAGATCCCATGC+TGG - Chr5:3302265-3302284 None:intergenic 50.0%
AGGAGTGAGTGTATGAAGGG+TGG - Chr5:3300270-3300289 None:intergenic 50.0%
ATAGAAGACATTGGGAGGGG+AGG + Chr5:3301516-3301535 MsG0580024316.01.T01:intron 50.0%
ATAGAGGAGTTTGAGAGGGG+AGG - Chr5:3301340-3301359 None:intergenic 50.0%
GAGACGAGAGTTACGGTTAG+AGG - Chr5:3300486-3300505 None:intergenic 50.0%
GCCAGAGGAAGATGACTTGA+TGG + Chr5:3300346-3300365 MsG0580024316.01.T01:CDS 50.0%
GCCGAAGATTGTTCCAGCAT+GGG + Chr5:3302249-3302268 MsG0580024316.01.T01:intron 50.0%
GGAGCAAGTGGAGGAAATAG+AGG - Chr5:3301356-3301375 None:intergenic 50.0%
GGGAGTTTAGAGGGAAGTGA+AGG + Chr5:3301455-3301474 MsG0580024316.01.T01:intron 50.0%
GTTGATTGGGCTTTCAGCAG+TGG + Chr5:3303063-3303082 MsG0580024316.01.T01:CDS 50.0%
GTTTGGGAGTTTAGAGGGCA+AGG - Chr5:3301410-3301429 None:intergenic 50.0%
TAGAAGACATTGGGAGGGGA+GGG + Chr5:3301517-3301536 MsG0580024316.01.T01:intron 50.0%
TAGAGGAGTTTGAGAGGGGA+GGG - Chr5:3301339-3301358 None:intergenic 50.0%
TTGAGTTCCCCCAAATGAGG+GGG - Chr5:3301577-3301596 None:intergenic 50.0%
TTTGGGAACGGAAGTGATGG+CGG - Chr5:3300426-3300445 None:intergenic 50.0%
! GCGGATTTGAGTTTGGGATG+TGG - Chr5:3300407-3300426 None:intergenic 50.0%
!! AACTCTCGTCTCGCTGATTC+CGG + Chr5:3300494-3300513 MsG0580024316.01.T01:CDS 50.0%
!! AGCTTTGGATTTCGAGCCAG+AGG + Chr5:3300331-3300350 MsG0580024316.01.T01:CDS 50.0%
!! GTTAACCTTCTGGTTTCCCG+AGG + Chr5:3301766-3301785 MsG0580024316.01.T01:intron 50.0%
!!! ATTGTAGGGTTTTGGAGGGG+AGG + Chr5:3300979-3300998 MsG0580024316.01.T01:intron 50.0%
!!! TTGTAGGGTTTTGGAGGGGA+GGG + Chr5:3300980-3300999 MsG0580024316.01.T01:intron 50.0%
AGTTTGAGAGGGGAGGGCTT+TGG - Chr5:3301333-3301352 None:intergenic 55.0%
ATCCAAAGCTTCTCCGTCGG+CGG - Chr5:3300321-3300340 None:intergenic 55.0%
CCTTTCCTCGGGAAACCAGA+AGG - Chr5:3301774-3301793 None:intergenic 55.0%
GATACTCTCACTGCTGAGGG+TGG + Chr5:3300521-3300540 MsG0580024316.01.T01:CDS 55.0%
GGAGTTTAGAGGGCAAGGGA+GGG - Chr5:3301405-3301424 None:intergenic 55.0%
GGCCGAAGATTGTTCCAGCA+TGG + Chr5:3302248-3302267 MsG0580024316.01.T01:intron 55.0%
GGGATGTGGAGGAGAAGCTT+CGG - Chr5:3300393-3300412 None:intergenic 55.0%
GTTTGAGAGGGGAGGGCTTT+GGG - Chr5:3301332-3301351 None:intergenic 55.0%
TTTGAGAGGGGAGGGCTTTG+GGG - Chr5:3301331-3301350 None:intergenic 55.0%
! ATACTGCAGGGAGTGCAGTG+AGG - Chr5:3302488-3302507 None:intergenic 55.0%
! ATTGGGAGGGGAGGGCTTTT+GGG + Chr5:3301525-3301544 MsG0580024316.01.T01:intron 55.0%
! TAGAGGGCAAGGGAGGGTTT+TGG - Chr5:3301399-3301418 None:intergenic 55.0%
!! ATGAGCGCTCTGAGGAAGCT+CGG + Chr5:3302989-3303008 MsG0580024316.01.T01:CDS 55.0%
!! CCTTCTGGTTTCCCGAGGAA+AGG + Chr5:3301771-3301790 MsG0580024316.01.T01:intron 55.0%
!! GTTGTTGGGAGGGGTTTTGG+AGG - Chr5:3301149-3301168 None:intergenic 55.0%
!!! TGTAGGGTTTTGGAGGGGAG+GGG + Chr5:3300981-3301000 MsG0580024316.01.T01:intron 55.0%
CAAAGGTCGCGGTTTCCGTC+AGG + Chr5:3300451-3300470 MsG0580024316.01.T01:CDS 60.0%
CTGACGGAAACCGCGACCTT+TGG - Chr5:3300453-3300472 None:intergenic 60.0%
GGGAGTTTAGAGGGCAAGGG+AGG - Chr5:3301406-3301425 None:intergenic 60.0%
! CATTGGGAGGGGAGGGCTTT+TGG + Chr5:3301524-3301543 MsG0580024316.01.T01:intron 60.0%
! TTGAGAGGGGAGGGCTTTGG+GGG - Chr5:3301330-3301349 None:intergenic 60.0%
! TTGGGAGGGGAGGGCTTTTG+GGG + Chr5:3301526-3301545 MsG0580024316.01.T01:intron 60.0%
!!! GTAGGGTTTTGGAGGGGAGG+GGG + Chr5:3300982-3301001 MsG0580024316.01.T01:intron 60.0%
CTCACTGCTGAGGGTGGTCC+TGG + Chr5:3300527-3300546 MsG0580024316.01.T01:CDS 65.0%
!! ATGCAAGGAAGCGCCGCCGA+CGG + Chr5:3300305-3300324 MsG0580024316.01.T01:CDS 65.0%
!! TGAGAGGGGAGGGCTTTGGG+GGG - Chr5:3301329-3301348 None:intergenic 65.0%
!! TTTTGGAGGGGAGGGGGTGG+AGG + Chr5:3300988-3301007 MsG0580024316.01.T01:intron 65.0%
CGCCGCCGACGGAGAAGCTT+TGG + Chr5:3300316-3300335 MsG0580024316.01.T01:CDS 70.0%
!!! GGGTTTTGGAGGGGAGGGGG+TGG + Chr5:3300985-3301004 MsG0580024316.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 3300223 3303119 3300223 ID=MsG0580024316.01;Name=MsG0580024316.01
Chr5 mRNA 3300223 3303119 3300223 ID=MsG0580024316.01.T01;Parent=MsG0580024316.01;Name=MsG0580024316.01.T01;_AED=0.44;_eAED=0.44;_QI=82|1|0.66|1|1|1|3|0|180
Chr5 exon 3300223 3300560 3300223 ID=MsG0580024316.01.T01:exon:1032;Parent=MsG0580024316.01.T01
Chr5 exon 3300651 3300787 3300651 ID=MsG0580024316.01.T01:exon:1033;Parent=MsG0580024316.01.T01
Chr5 exon 3302970 3303119 3302970 ID=MsG0580024316.01.T01:exon:1034;Parent=MsG0580024316.01.T01
Chr5 five_prime_UTR 3300223 3300304 3300223 ID=MsG0580024316.01.T01:five_prime_utr;Parent=MsG0580024316.01.T01
Chr5 CDS 3300305 3300560 3300305 ID=MsG0580024316.01.T01:cds;Parent=MsG0580024316.01.T01
Chr5 CDS 3300651 3300787 3300651 ID=MsG0580024316.01.T01:cds;Parent=MsG0580024316.01.T01
Chr5 CDS 3302970 3303119 3302970 ID=MsG0580024316.01.T01:cds;Parent=MsG0580024316.01.T01
Gene Sequence

>MsG0580024316.01.T01

ATGCAAGGAAGCGCCGCCGACGGAGAAGCTTTGGATTTCGAGCCAGAGGAAGATGACTTGATGGACGAAGAAGGTGCTGTTGACGCCGAAGCTTCTCCTCCACATCCCAAACTCAAATCCGCCATCACTTCCGTTCCCAAAAAAACCAAAGGTCGCGGTTTCCGTCAGGATTCCGATTCCTCTAACCGTAACTCTCGTCTCGCTGATTCCGGTTTCGATACTCTCACTGCTGAGGGTGGTCCTGGTCCTCAAAGATCTATTGAAGGATGGATTATTTTGGTGACTGGTGTACATGAGGAAGCTCAAGATGATGATTTGCAGAATGCTTTTGGTGAATATGGTGATATTAAGAATCTTCACTTGAATCTTGATCGCCGTACTGGTTTTGTTAAGGGTTATGCACTGATTGAATATGAGCGCTCTGAGGAAGCTCGGAATGCAATAGAGAATTTGAATGGATCTGTGCTTCTCACACAAACCATTTATGTTGATTGGGCTTTCAGCAGTGGACCTATCAATGAGTCAGTTAGAAGAAACAGGTAA
Protein sequence

>MsG0580024316.01.T01

MQGSAADGEALDFEPEEDDLMDEEGAVDAEASPPHPKLKSAITSVPKKTKGRGFRQDSDSSNRNSRLADSGFDTLTAEGGPGPQRSIEGWIILVTGVHEEAQDDDLQNAFGEYGDIKNLHLNLDRRTGFVKGYALIEYERSEEARNAIENLNGSVLLTQTIYVDWAFSSGPINESVRRNR*