AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024362.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024362.01.T01 MTR_5g009720 81.356 118 12 3 78 195 13 120 1.16e-53 171
MsG0580024362.01.T01 MTR_8g099190 60.156 128 31 2 78 195 13 130 4.39e-41 139
MsG0580024362.01.T01 MTR_8g099190 60.156 128 31 2 78 195 13 130 5.25e-41 139
MsG0580024362.01.T01 MTR_8g099190 60.156 128 31 2 78 195 13 130 7.28e-41 138
MsG0580024362.01.T01 MTR_8g099190 60.156 128 31 2 78 195 13 130 9.52e-41 139
MsG0580024362.01.T01 MTR_4g073800 61.017 118 36 3 78 195 13 120 6.09e-38 132
MsG0580024362.01.T01 MTR_5g033180 66.250 80 21 1 1 74 49 128 9.85e-28 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024362.01.T01 AT4G11150 66.949 118 29 3 78 195 13 120 5.41e-43 144
MsG0580024362.01.T01 AT1G64200 64.516 124 28 3 78 195 13 126 1.04e-41 139
MsG0580024362.01.T01 AT1G64200 64.516 124 28 3 78 195 13 126 1.71e-41 140
MsG0580024362.01.T01 AT1G64200 64.516 124 28 3 78 195 13 126 1.84e-41 140
MsG0580024362.01.T01 AT3G08560 57.627 118 40 3 78 195 13 120 5.51e-33 118

Find 40 sgRNAs with CRISPR-Local

Find 296 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGGATCAATTATGGTTT+TGG 0.145209 5:+3885294 MsG0580024362.01.T01:CDS
TCTAAAAGATCTCGTTATTC+AGG 0.260442 5:+3889811 MsG0580024362.01.T01:CDS
TGAGGGGTGCCCATCTGTTT+TGG 0.283128 5:+3885221 MsG0580024362.01.T01:CDS
CGAGATCGGAGTCAGTTCTT+TGG 0.300725 5:+3885277 MsG0580024362.01.T01:CDS
TCAGTTCTTTGGATCAATTA+TGG 0.324208 5:+3885288 MsG0580024362.01.T01:CDS
GGCGTGATTTGGTATTTCTT+TGG 0.342113 5:+3885187 MsG0580024362.01.T01:CDS
TGATCGGCCCACCAGACTTT+AGG 0.359831 5:-3885245 None:intergenic
CTTTGCGCTCATACTCTTGA+CGG 0.390529 5:-3888859 None:intergenic
TTTCGGATGTGGCTCAGCTT+TGG 0.407410 5:+3885391 MsG0580024362.01.T01:CDS
TTTATTAACCACATCATCTT+GGG 0.412688 5:-3889723 None:intergenic
GTGGAAGCTGAATGATCTTT+CGG 0.432636 5:+3885359 MsG0580024362.01.T01:CDS
GTTGAATGCTTCACGGTTGA+AGG 0.443148 5:+3889691 MsG0580024362.01.T01:CDS
CTATGCAGTTGAATGCTTCA+CGG 0.456350 5:+3889684 MsG0580024362.01.T01:CDS
ATGTGGCTCAGCTTTGGTGG+TGG 0.467610 5:+3885397 MsG0580024362.01.T01:CDS
CGAGAAGTTGCAGTTGGTTG+AGG 0.469869 5:+3888820 MsG0580024362.01.T01:CDS
TCTTTGGTGTACTGAGGGTG+AGG 0.472309 5:+3885203 MsG0580024362.01.T01:CDS
TGTGCTGGTTGCCGGTGGGC+CGG 0.481651 5:+3885115 MsG0580024362.01.T01:CDS
CGGATGTGGCTCAGCTTTGG+TGG 0.489587 5:+3885394 MsG0580024362.01.T01:CDS
GATCTCGAATCAAAATTAAC+CGG 0.489604 5:-3885134 None:intergenic
TGTGGCTCAGCTTTGGTGGT+GGG 0.491458 5:+3885398 MsG0580024362.01.T01:CDS
GATCAGAGGTTGAGGAGTGG+TGG 0.493012 5:+3885340 MsG0580024362.01.T01:CDS
CTTTGGTGTACTGAGGGTGA+GGG 0.503297 5:+3885204 MsG0580024362.01.T01:CDS
ATCAAAATTAACCGGCCCAC+CGG 0.504320 5:-3885126 None:intergenic
TTTCAGTGGATCAGAGGTTG+AGG 0.505992 5:+3885332 MsG0580024362.01.T01:CDS
CAATATCGAGAAGTTGCAGT+TGG 0.528790 5:+3888814 MsG0580024362.01.T01:CDS
GTATTTCTTTGGTGTACTGA+GGG 0.529175 5:+3885198 MsG0580024362.01.T01:CDS
GGTTTCTTTCAGTGGATCAG+AGG 0.529763 5:+3885326 MsG0580024362.01.T01:CDS
GCTCACATTCAAGAGTTCCT+TGG 0.530777 5:-3889762 None:intergenic
CACATTCAAGAGTTCCTTGG+CGG 0.533307 5:-3889759 None:intergenic
GACTTTAGGACCAAAACAGA+TGG 0.555135 5:-3885231 None:intergenic
TTTGGTGTACTGAGGGTGAG+GGG 0.561993 5:+3885205 MsG0580024362.01.T01:CDS
GGTATTTCTTTGGTGTACTG+AGG 0.566415 5:+3885197 MsG0580024362.01.T01:CDS
AATGAAAGAGTCTGCCGCCA+AGG 0.574070 5:+3889745 MsG0580024362.01.T01:CDS
TTTGATTCGAGATCGGGAGT+TGG 0.593537 5:+3885143 MsG0580024362.01.T01:CDS
TTTGTGTGCTGGTTGCCGGT+GGG 0.611277 5:+3885111 MsG0580024362.01.T01:CDS
TCTTCAAGCCCAAGATGATG+TGG 0.617482 5:+3889715 MsG0580024362.01.T01:CDS
AGCTGAATGATCTTTCGGAT+AGG 0.647786 5:+3885364 MsG0580024362.01.T01:CDS
ACTTTAGGACCAAAACAGAT+GGG 0.657623 5:-3885230 None:intergenic
GATCTCGAATCAAAAGTGAT+CGG 0.665266 5:-3885261 None:intergenic
GTGGATCAGAGGTTGAGGAG+TGG 0.693970 5:+3885337 MsG0580024362.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACTAAATTCAAATATATT+CGG - Chr5:3889284-3889303 None:intergenic 10.0%
!! ATGAAAAAAAAATATGTAAA+AGG - Chr5:3888919-3888938 None:intergenic 10.0%
!! TAATTTAATAAGATTGTATT+TGG + Chr5:3886176-3886195 MsG0580024362.01.T01:intron 10.0%
!! TACAATCTTATTAAATTAAA+TGG - Chr5:3886174-3886193 None:intergenic 10.0%
!!! AAACATTTTTTATTAAACTA+TGG - Chr5:3887715-3887734 None:intergenic 10.0%
!!! TAAGTGTTATAATAATATTT+TGG - Chr5:3887681-3887700 None:intergenic 10.0%
!!! TTTTAATGATTTTTTTTACT+TGG - Chr5:3886071-3886090 None:intergenic 10.0%
!! ATTTATTGATATATGTGAAA+AGG + Chr5:3888402-3888421 MsG0580024362.01.T01:intron 15.0%
!!! ATATATCAGAAATAGTTTTA+TGG - Chr5:3887892-3887911 None:intergenic 15.0%
!!! CAAATGTTTCTTTAAAAAAA+TGG - Chr5:3886325-3886344 None:intergenic 15.0%
!!! CACTTAACATATTTTTTTAA+CGG - Chr5:3887345-3887364 None:intergenic 15.0%
!!! CATATAGTTTTAGATTTATT+TGG + Chr5:3889048-3889067 MsG0580024362.01.T01:intron 15.0%
!!! TGTCGAAATTTTTAAAATTT+CGG + Chr5:3886353-3886372 MsG0580024362.01.T01:intron 15.0%
!!! TTTTAAAGAAACATTTGTAT+GGG + Chr5:3886328-3886347 MsG0580024362.01.T01:intron 15.0%
!!! TTTTTAAAGAAACATTTGTA+TGG + Chr5:3886327-3886346 MsG0580024362.01.T01:intron 15.0%
!!! TTTTTATGGTGTTTATTTTT+CGG + Chr5:3885549-3885568 MsG0580024362.01.T01:intron 15.0%
!!! TTTTTGCTTATAATAGTTAT+CGG - Chr5:3887179-3887198 None:intergenic 15.0%
!! AATAAAAATATACACAGAGA+TGG - Chr5:3887853-3887872 None:intergenic 20.0%
!! AATTATTAATCTATTTGCCA+AGG + Chr5:3887018-3887037 MsG0580024362.01.T01:intron 20.0%
!! AATTGATCAGAAAACTTATA+TGG + Chr5:3888944-3888963 MsG0580024362.01.T01:intron 20.0%
!! ACTAAAGTGAAAGTAAAAAA+TGG + Chr5:3887406-3887425 MsG0580024362.01.T01:intron 20.0%
!! ACTAAAGTGAAAGTAAAAAA+TGG - Chr5:3887425-3887406 None:intergenic 20.0%
!! ATCCAACTATTATAATTGAA+TGG - Chr5:3889112-3889131 None:intergenic 20.0%
!! ATGACTTAATAGTTATGTTA+TGG - Chr5:3887738-3887757 None:intergenic 20.0%
!! CATTCAATAAATGATGTATA+TGG + Chr5:3888356-3888375 MsG0580024362.01.T01:intron 20.0%
!! CATTTATTAAATGATGTATG+CGG - Chr5:3886732-3886751 None:intergenic 20.0%
!! CTTCATATATTTATCTAATG+AGG + Chr5:3885933-3885952 MsG0580024362.01.T01:intron 20.0%
!! GAAAAAGTGAAATTAAGAAA+TGG - Chr5:3888691-3888710 None:intergenic 20.0%
!! TCAATTATAATAGTTGGATT+TGG + Chr5:3889113-3889132 MsG0580024362.01.T01:intron 20.0%
!! TTATATTTAATCCTGAATCA+GGG + Chr5:3888283-3888302 MsG0580024362.01.T01:intron 20.0%
!! TTTAAAGAAACATTTGTATG+GGG + Chr5:3886329-3886348 MsG0580024362.01.T01:intron 20.0%
!! TTTAATAAGATTGTATTTGG+TGG + Chr5:3886179-3886198 MsG0580024362.01.T01:intron 20.0%
!! TTTATATTTAATCCTGAATC+AGG + Chr5:3888282-3888301 MsG0580024362.01.T01:intron 20.0%
!!! CACTTAACAGATTTTTTTAA+CGG + Chr5:3888217-3888236 MsG0580024362.01.T01:intron 20.0%
!!! GTGATAATAAGAAATTTGTT+TGG + Chr5:3886426-3886445 MsG0580024362.01.T01:intron 20.0%
!!! TAATAAGAAATTTGTTTGGA+AGG + Chr5:3886430-3886449 MsG0580024362.01.T01:intron 20.0%
!!! TATCGTTGATTAATAGAATT+TGG + Chr5:3889476-3889495 MsG0580024362.01.T01:intron 20.0%
!!! TCTTTTTCTAACTTGAAAAA+TGG + Chr5:3889609-3889628 MsG0580024362.01.T01:intron 20.0%
!!! TTGTTATTTACATATGTGAA+TGG + Chr5:3888968-3888987 MsG0580024362.01.T01:intron 20.0%
!!! TTTTATGGTGTTTATTTTTC+GGG + Chr5:3885550-3885569 MsG0580024362.01.T01:intron 20.0%
! AAAGTAAAAAATGGTCACTT+TGG + Chr5:3887415-3887434 MsG0580024362.01.T01:intron 25.0%
! AAAGTAAAAAATGGTCACTT+TGG - Chr5:3887434-3887415 None:intergenic 25.0%
! AATATCCTCAACTTTATGTT+AGG - Chr5:3888035-3888054 None:intergenic 25.0%
! ACGATCTAATAAACAAAACA+TGG - Chr5:3888756-3888775 None:intergenic 25.0%
! AGTGAAATTAAGAAATGGAT+CGG - Chr5:3888686-3888705 None:intergenic 25.0%
! ATTTAATCAGTCATCATACT+TGG - Chr5:3889508-3889527 None:intergenic 25.0%
! CAAATATAAATGCCCAAATA+TGG - Chr5:3887794-3887813 None:intergenic 25.0%
! GATGTATATGGTCTATATAT+AGG + Chr5:3888368-3888387 MsG0580024362.01.T01:intron 25.0%
! TCAATTGTATAAGATCATCA+AGG + Chr5:3889021-3889040 MsG0580024362.01.T01:intron 25.0%
! TGCCATTCAATTATAATAGT+TGG + Chr5:3889107-3889126 MsG0580024362.01.T01:intron 25.0%
! TTAAAGAAACATTTGTATGG+GGG + Chr5:3886330-3886349 MsG0580024362.01.T01:intron 25.0%
! TTTATTAACCACATCATCTT+GGG - Chr5:3889726-3889745 None:intergenic 25.0%
!! AAATATGTTAAGTGATGATG+TGG + Chr5:3887351-3887370 MsG0580024362.01.T01:intron 25.0%
!! TACTTGGAACTTTTAATATG+AGG - Chr5:3886055-3886074 None:intergenic 25.0%
!! TTTGGATCAATTATGGTTTT+GGG + Chr5:3885295-3885314 MsG0580024362.01.T01:CDS 25.0%
!! TTTTATTAACCACATCATCT+TGG - Chr5:3889727-3889746 None:intergenic 25.0%
!! TTTTCTAACTTGAAAAATGG+TGG + Chr5:3889612-3889631 MsG0580024362.01.T01:intron 25.0%
!!! AACATATTTTTTTAACGGCA+GGG - Chr5:3887340-3887359 None:intergenic 25.0%
!!! AGGATATTGATTTTCGATTT+AGG - Chr5:3887443-3887462 None:intergenic 25.0%
!!! GGATATTGATTTTCGATTTA+GGG - Chr5:3887442-3887461 None:intergenic 25.0%
!!! TAACATATTTTTTTAACGGC+AGG - Chr5:3887341-3887360 None:intergenic 25.0%
!!! TTTTTTCCTAATCTTTACCT+TGG - Chr5:3887127-3887146 None:intergenic 25.0%
AAATTTCGTGTTTACTGTCA+GGG + Chr5:3887976-3887995 MsG0580024362.01.T01:intron 30.0%
AATATCCTCAACTTACTGTT+AGG + Chr5:3887527-3887546 MsG0580024362.01.T01:intron 30.0%
AATGACCTAACATAAAGTTG+AGG + Chr5:3888027-3888046 MsG0580024362.01.T01:intron 30.0%
ACAATAAGTATACTCAGTCA+GGG + Chr5:3888080-3888099 MsG0580024362.01.T01:intron 30.0%
ACAATGCATAATATATGCAG+GGG + Chr5:3886894-3886913 MsG0580024362.01.T01:intron 30.0%
AGGGATCTAACATTAAATTG+AGG - Chr5:3887463-3887482 None:intergenic 30.0%
AGGGATTTAACAGTAAATTG+AGG + Chr5:3888099-3888118 MsG0580024362.01.T01:intron 30.0%
ATTTGCCAAGGTAAAAGTTA+GGG + Chr5:3887030-3887049 MsG0580024362.01.T01:intron 30.0%
CAAAAGATTGCACCATATTT+GGG + Chr5:3887779-3887798 MsG0580024362.01.T01:intron 30.0%
CTCTAAGTAAGTACTAATCA+CGG + Chr5:3886379-3886398 MsG0580024362.01.T01:intron 30.0%
GACAATGCATAATATATGCA+GGG + Chr5:3886893-3886912 MsG0580024362.01.T01:intron 30.0%
GACATTGTGTTATATATGCA+AGG + Chr5:3885888-3885907 MsG0580024362.01.T01:intron 30.0%
GAGTATACTTATTGTCAGTT+AGG - Chr5:3888075-3888094 None:intergenic 30.0%
GATCCAACTATTGTAATTGA+AGG - Chr5:3889260-3889279 None:intergenic 30.0%
GATCTCGAATCAAAATTAAC+CGG - Chr5:3885137-3885156 None:intergenic 30.0%
GTGTATATTTACTGTCAGTT+AGG + Chr5:3887559-3887578 MsG0580024362.01.T01:intron 30.0%
TATACATGTATACACCCATT+CGG + Chr5:3886636-3886655 MsG0580024362.01.T01:intron 30.0%
TATTTGCCAAGGTAAAAGTT+AGG + Chr5:3887029-3887048 MsG0580024362.01.T01:intron 30.0%
TATTTGCCAAGGTAAAGATT+AGG + Chr5:3887118-3887137 MsG0580024362.01.T01:intron 30.0%
TCAAAAGATTGCACCATATT+TGG + Chr5:3887778-3887797 MsG0580024362.01.T01:intron 30.0%
TCAGTTCTTTGGATCAATTA+TGG + Chr5:3885288-3885307 MsG0580024362.01.T01:CDS 30.0%
TCGATCATCAAATTCAAAGT+TGG + Chr5:3886022-3886041 MsG0580024362.01.T01:intron 30.0%
TCTAAAAGATCTCGTTATTC+AGG + Chr5:3889811-3889830 MsG0580024362.01.T01:CDS 30.0%
TCTTCAAGTAGTCTAATTGT+TGG - Chr5:3886973-3886992 None:intergenic 30.0%
TGCTTATAATAGTTATCGGA+AGG - Chr5:3887175-3887194 None:intergenic 30.0%
TGTCCTTCAATTACAATAGT+TGG + Chr5:3889254-3889273 MsG0580024362.01.T01:intron 30.0%
TTACGCCTTAAGAACATTTA+TGG + Chr5:3885746-3885765 MsG0580024362.01.T01:intron 30.0%
TTGTATTTAATCCTGAGTCA+GGG - Chr5:3887279-3887298 None:intergenic 30.0%
TTTGTATTTAATCCTGAGTC+AGG - Chr5:3887280-3887299 None:intergenic 30.0%
! AAATCTGTTAAGTGATGATG+TGG - Chr5:3888211-3888230 None:intergenic 30.0%
! AGAAATTTGTTTGGAAGGTA+AGG + Chr5:3886435-3886454 MsG0580024362.01.T01:intron 30.0%
! CTTTGGATCAATTATGGTTT+TGG + Chr5:3885294-3885313 MsG0580024362.01.T01:CDS 30.0%
! CTTTTTATGGTTTCTTTCAG+TGG + Chr5:3885318-3885337 MsG0580024362.01.T01:CDS 30.0%
! GAATAACGAGATCTTTTAGA+AGG - Chr5:3889811-3889830 None:intergenic 30.0%
! GATCTTTTCTCATAGATCAT+TGG + Chr5:3889419-3889438 MsG0580024362.01.T01:intron 30.0%
! TACAATTGGTAGCAATTGAT+TGG + Chr5:3889334-3889353 MsG0580024362.01.T01:intron 30.0%
! TGAATGAGAGTACAAACAAA+AGG - Chr5:3885596-3885615 None:intergenic 30.0%
! TGGATGAGAAGTATATGATT+GGG - Chr5:3888438-3888457 None:intergenic 30.0%
! TTTTGTACACATGATGATCA+CGG - Chr5:3889787-3889806 None:intergenic 30.0%
! TTTTTCCCTAACTTTTACCT+TGG - Chr5:3887038-3887057 None:intergenic 30.0%
!! ACATTTTAAAGGTGAAGAAG+TGG - Chr5:3886943-3886962 None:intergenic 30.0%
!! CATTTTAAAGGTGAAGAAGT+GGG - Chr5:3886942-3886961 None:intergenic 30.0%
!! GTTAAACTAACAACTTGCTA+AGG + Chr5:3887944-3887963 MsG0580024362.01.T01:intron 30.0%
!!! AACAGATTTTTTTAACGGCA+GGG + Chr5:3888222-3888241 MsG0580024362.01.T01:intron 30.0%
!!! AGGATATTGATTTTCGATTC+AGG + Chr5:3888047-3888066 MsG0580024362.01.T01:intron 30.0%
!!! AGGATATTGATTTTCGATTC+AGG - Chr5:3888066-3888047 None:intergenic 30.0%
!!! GGATATTGATTTTCGATTCA+GGG + Chr5:3888048-3888067 MsG0580024362.01.T01:intron 30.0%
!!! GGATATTGATTTTCGATTCA+GGG - Chr5:3888067-3888048 None:intergenic 30.0%
!!! GGTTAATTTTGATTCGAGAT+CGG + Chr5:3885136-3885155 MsG0580024362.01.T01:CDS 30.0%
!!! GTTAATTTTGATTCGAGATC+GGG + Chr5:3885137-3885156 MsG0580024362.01.T01:CDS 30.0%
!!! TAACAGATTTTTTTAACGGC+AGG + Chr5:3888221-3888240 MsG0580024362.01.T01:intron 30.0%
!!! TTATGGTTTTGGGCTTTTTA+TGG + Chr5:3885305-3885324 MsG0580024362.01.T01:CDS 30.0%
AAATTTCGTGCTTACTGTCA+GGG - Chr5:3887586-3887605 None:intergenic 35.0%
AATGACCTAACAGTAAGTTG+AGG - Chr5:3887535-3887554 None:intergenic 35.0%
ACAGTAAGTATACACAGTCA+GGG - Chr5:3888027-3888008 None:intergenic 35.0%
CAACGATATGTGATTAGTAC+TGG - Chr5:3889464-3889483 None:intergenic 35.0%
CATCCAAAACGAGTTTACTT+TGG - Chr5:3889207-3889226 None:intergenic 35.0%
CGAAATTTCACTAAGATCCT+TGG + Chr5:3887598-3887617 MsG0580024362.01.T01:intron 35.0%
CTCCACCATAAATGTTCTTA+AGG - Chr5:3885754-3885773 None:intergenic 35.0%
GAAATTTCGTGTTTACTGTC+AGG + Chr5:3887975-3887994 MsG0580024362.01.T01:intron 35.0%
GACAATAAGTATACTCAGTC+AGG + Chr5:3888079-3888098 MsG0580024362.01.T01:intron 35.0%
GAGGAAACTCTAACTTGATT+AGG - Chr5:3886588-3886607 None:intergenic 35.0%
GATCTCGAATCAAAAGTGAT+CGG - Chr5:3885264-3885283 None:intergenic 35.0%
GCTTATAATAGTGACCGAAT+GGG - Chr5:3886653-3886672 None:intergenic 35.0%
GGACAATGCATAATATATGC+AGG + Chr5:3886892-3886911 MsG0580024362.01.T01:intron 35.0%
GTGTATACTTACTGTCAGTT+AGG + Chr5:3887487-3887506 MsG0580024362.01.T01:intron 35.0%
GTGTATACTTACTGTCAGTT+AGG - Chr5:3887506-3887487 None:intergenic 35.0%
GTTAAACTAACAACTTGCCA+AGG - Chr5:3887618-3887637 None:intergenic 35.0%
TCGAGTTACAAACTATGATG+TGG + Chr5:3886137-3886156 MsG0580024362.01.T01:intron 35.0%
TGAGCTTAGTTTAGTCGTTA+GGG + Chr5:3885866-3885885 MsG0580024362.01.T01:intron 35.0%
TGCTTATAATAGTGACCGAA+TGG - Chr5:3886654-3886673 None:intergenic 35.0%
TTGCGTGGGTAGTAAATAAA+TGG + Chr5:3885830-3885849 MsG0580024362.01.T01:intron 35.0%
TTGTTTGTACTCTCATTCAC+CGG + Chr5:3885597-3885616 MsG0580024362.01.T01:intron 35.0%
! CATCCAAAGTAAACTCGTTT+TGG + Chr5:3889201-3889220 MsG0580024362.01.T01:intron 35.0%
! GTATTTCTTTGGTGTACTGA+GGG + Chr5:3885198-3885217 MsG0580024362.01.T01:CDS 35.0%
! GTGGATGAGAAGTATATGAT+TGG - Chr5:3888439-3888458 None:intergenic 35.0%
! TCTTTCGGATAGGAACTTTT+CGG + Chr5:3885374-3885393 MsG0580024362.01.T01:CDS 35.0%
! TTGGAGCTCACACATTTTAA+AGG - Chr5:3886954-3886973 None:intergenic 35.0%
!! ACTTTAGGACCAAAACAGAT+GGG - Chr5:3885233-3885252 None:intergenic 35.0%
!! GAATTACGCTGTTTTGTTGT+AGG + Chr5:3888787-3888806 MsG0580024362.01.T01:intron 35.0%
!! GATCACTTTTGATTCGAGAT+CGG + Chr5:3885263-3885282 MsG0580024362.01.T01:CDS 35.0%
!! GCAATCTTTTGATTACTCTG+CGG - Chr5:3887770-3887789 None:intergenic 35.0%
!! GTTGGATATCGTTTTTACAG+TGG + Chr5:3885458-3885477 MsG0580024362.01.T01:intron 35.0%
AGCTGAATGATCTTTCGGAT+AGG + Chr5:3885364-3885383 MsG0580024362.01.T01:CDS 40.0%
ATGTTCAAGACGAACAAGAC+CGG - Chr5:3885619-3885638 None:intergenic 40.0%
CAACCAGCACACAAAAAGTA+TGG - Chr5:3885106-3885125 None:intergenic 40.0%
CAATATCGAGAAGTTGCAGT+TGG + Chr5:3888814-3888833 MsG0580024362.01.T01:CDS 40.0%
CACAGTCTCACAGATACAAT+TGG + Chr5:3889320-3889339 MsG0580024362.01.T01:intron 40.0%
CATAATATATGCAGGGGTCA+GGG + Chr5:3886900-3886919 MsG0580024362.01.T01:intron 40.0%
CGCCTTAAGAACATTTATGG+TGG + Chr5:3885749-3885768 MsG0580024362.01.T01:intron 40.0%
CTATGCAGTTGAATGCTTCA+CGG + Chr5:3889684-3889703 MsG0580024362.01.T01:CDS 40.0%
GAAATTTCGTGCTTACTGTC+AGG - Chr5:3887587-3887606 None:intergenic 40.0%
GAAGTGATGTACTTCTTCAG+CGG - Chr5:3888660-3888679 None:intergenic 40.0%
GACAGTAAGTATACACAGTC+AGG - Chr5:3888026-3888007 None:intergenic 40.0%
GCTACTTACTGACAACACTA+AGG + Chr5:3887640-3887659 MsG0580024362.01.T01:intron 40.0%
GCTACTTACTGACAACACTA+AGG - Chr5:3887659-3887640 None:intergenic 40.0%
GTGAGCTTAGTTTAGTCGTT+AGG + Chr5:3885865-3885884 MsG0580024362.01.T01:intron 40.0%
GTGGAAGCTGAATGATCTTT+CGG + Chr5:3885359-3885378 MsG0580024362.01.T01:CDS 40.0%
GTTCGTCTTGAACATGGATT+TGG + Chr5:3885624-3885643 MsG0580024362.01.T01:intron 40.0%
TATCACGATCCATTTACCGT+GGG - Chr5:3886412-3886431 None:intergenic 40.0%
TATTAACCGAGCTAAGCTCA+CGG - Chr5:3886871-3886890 None:intergenic 40.0%
TGAGCTTAGCTCGGTTAATA+GGG + Chr5:3886871-3886890 MsG0580024362.01.T01:intron 40.0%
TTATCACGATCCATTTACCG+TGG - Chr5:3886413-3886432 None:intergenic 40.0%
TTGGAAGAATAGCAGTGATG+TGG + Chr5:3885807-3885826 MsG0580024362.01.T01:intron 40.0%
! ATAACGGAAGTGTAGAGTCA+GGG + Chr5:3888254-3888273 MsG0580024362.01.T01:intron 40.0%
! ATAACGGAAGTGTAGAGTCA+GGG - Chr5:3888273-3888254 None:intergenic 40.0%
! CGATATGTGATTAGTACTGG+CGG - Chr5:3889461-3889480 None:intergenic 40.0%
! CTCGTGTAGGAGTCAATTTT+AGG + Chr5:3885486-3885505 MsG0580024362.01.T01:intron 40.0%
! GGTATTTCTTTGGTGTACTG+AGG + Chr5:3885197-3885216 MsG0580024362.01.T01:CDS 40.0%
! GTGTAGGAGTCAATTTTAGG+TGG + Chr5:3885489-3885508 MsG0580024362.01.T01:intron 40.0%
! TTTTGGTCCTAAAGTCTGGT+GGG + Chr5:3885238-3885257 MsG0580024362.01.T01:CDS 40.0%
!! AAACAAAAGGCGCCGTATAT+AGG - Chr5:3885583-3885602 None:intergenic 40.0%
!! AAGAGCATCTACAATGACAG+AGG - Chr5:3886607-3886626 None:intergenic 40.0%
!! GACTTTAGGACCAAAACAGA+TGG - Chr5:3885234-3885253 None:intergenic 40.0%
!! GATATGTGATTAGTACTGGC+GGG - Chr5:3889460-3889479 None:intergenic 40.0%
!! GGCGTGATTTGGTATTTCTT+TGG + Chr5:3885187-3885206 MsG0580024362.01.T01:CDS 40.0%
!! TTAGGTGGTGATGCGTATTT+TGG + Chr5:3885504-3885523 MsG0580024362.01.T01:intron 40.0%
!! TTGCCATACTTTTTGTGTGC+TGG + Chr5:3885100-3885119 MsG0580024362.01.T01:CDS 40.0%
!!! CAGTTTTGTTTGGCGTGATT+TGG + Chr5:3885176-3885195 MsG0580024362.01.T01:CDS 40.0%
!!! TCTGTTTTGGTCCTAAAGTC+TGG + Chr5:3885234-3885253 MsG0580024362.01.T01:CDS 40.0%
!!! TGTCTAGCTGCAGTTTTGTT+TGG + Chr5:3885166-3885185 MsG0580024362.01.T01:CDS 40.0%
AGAAGATCGATCGAGAGAGA+GGG - Chr5:3888513-3888532 None:intergenic 45.0%
AGAGGGGATTGGAATCGAAT+CGG - Chr5:3888536-3888555 None:intergenic 45.0%
ATCAAAATTAACCGGCCCAC+CGG - Chr5:3885129-3885148 None:intergenic 45.0%
ATTAACCGAGCTAAGCTCAC+GGG - Chr5:3886870-3886889 None:intergenic 45.0%
CACATTCAAGAGTTCCTTGG+CGG - Chr5:3889762-3889781 None:intergenic 45.0%
CACCATAAAAAACACCCCTG+AGG - Chr5:3885540-3885559 None:intergenic 45.0%
CGTCTTTGTAAGACTGTCTC+CGG - Chr5:3886212-3886231 None:intergenic 45.0%
CTTTGCGCTCATACTCTTGA+CGG - Chr5:3888862-3888881 None:intergenic 45.0%
GCATAATATATGCAGGGGTC+AGG + Chr5:3886899-3886918 MsG0580024362.01.T01:intron 45.0%
GCTCACATTCAAGAGTTCCT+TGG - Chr5:3889765-3889784 None:intergenic 45.0%
GGCAGGGACTAAAGTGATAA+CGG + Chr5:3888238-3888257 MsG0580024362.01.T01:intron 45.0%
GGCAGGGACTAAAGTGATAA+CGG - Chr5:3888257-3888238 None:intergenic 45.0%
GGTTTCTTTCAGTGGATCAG+AGG + Chr5:3885326-3885345 MsG0580024362.01.T01:CDS 45.0%
GTGAGCTTAGCTCGGTTAAT+AGG + Chr5:3886870-3886889 MsG0580024362.01.T01:intron 45.0%
GTTGAATGCTTCACGGTTGA+AGG + Chr5:3889691-3889710 MsG0580024362.01.T01:CDS 45.0%
TAATCACGGTCTGATTCCCA+CGG + Chr5:3886393-3886412 MsG0580024362.01.T01:intron 45.0%
TCTTCAAGCCCAAGATGATG+TGG + Chr5:3889715-3889734 MsG0580024362.01.T01:CDS 45.0%
TTTCAGTGGATCAGAGGTTG+AGG + Chr5:3885332-3885351 MsG0580024362.01.T01:CDS 45.0%
! AAAGAGGCTTTTGTCGATCG+TGG - Chr5:3888465-3888484 None:intergenic 45.0%
! AAGGAGTATGACGTGTCACA+GGG + Chr5:3887383-3887402 MsG0580024362.01.T01:intron 45.0%
! AAGGAGTATGACGTGTCACA+GGG - Chr5:3887402-3887383 None:intergenic 45.0%
! CTTTTGTCGATCGTGGTTTG+TGG - Chr5:3888458-3888477 None:intergenic 45.0%
! GACATCTGCGTCGTTCATTT+TGG - Chr5:3888564-3888583 None:intergenic 45.0%
! GGATAGGAACTTTTCGGATG+TGG + Chr5:3885380-3885399 MsG0580024362.01.T01:CDS 45.0%
! TTTGATTCGAGATCGGGAGT+TGG + Chr5:3885143-3885162 MsG0580024362.01.T01:CDS 45.0%
!! GATAACGGAAGTGTAGAGTC+AGG + Chr5:3888253-3888272 MsG0580024362.01.T01:intron 45.0%
!! GATAACGGAAGTGTAGAGTC+AGG - Chr5:3888272-3888253 None:intergenic 45.0%
!! GATCTCGTTGGCTTTCTCTT+CGG - Chr5:3888630-3888649 None:intergenic 45.0%
!! GGTCTTGTTCGTCTTGAACA+TGG + Chr5:3885618-3885637 MsG0580024362.01.T01:intron 45.0%
!! GTTTTGGTCCTAAAGTCTGG+TGG + Chr5:3885237-3885256 MsG0580024362.01.T01:CDS 45.0%
!! TGGTGGGTGTTTTTCTTTGG+GGG + Chr5:3885414-3885433 MsG0580024362.01.T01:intron 45.0%
!! TGTATTTGGTGGTGCAACAC+CGG + Chr5:3886190-3886209 MsG0580024362.01.T01:intron 45.0%
!!! ACTTTTTGTGTGCTGGTTGC+CGG + Chr5:3885107-3885126 MsG0580024362.01.T01:CDS 45.0%
!!! GGTGGTGGGTGTTTTTCTTT+GGG + Chr5:3885412-3885431 MsG0580024362.01.T01:intron 45.0%
!!! GTCCTCAGGGGTGTTTTTTA+TGG + Chr5:3885535-3885554 MsG0580024362.01.T01:intron 45.0%
!!! GTGGTGATGCGTATTTTGGT+GGG + Chr5:3885508-3885527 MsG0580024362.01.T01:intron 45.0%
!!! GTGGTGGGTGTTTTTCTTTG+GGG + Chr5:3885413-3885432 MsG0580024362.01.T01:intron 45.0%
!!! TGGTGGTGGGTGTTTTTCTT+TGG + Chr5:3885411-3885430 MsG0580024362.01.T01:intron 45.0%
!!! ATTTTTAATCATTTTTTTAT+CGG - Chr5:3885726-3885745 None:intergenic 5.0%
!!! TTAGTTTTTTTTATTTTTAT+TGG + Chr5:3885788-3885807 MsG0580024362.01.T01:intron 5.0%
AATGAAAGAGTCTGCCGCCA+AGG + Chr5:3889745-3889764 MsG0580024362.01.T01:CDS 50.0%
AGCAAATCCAGCAGATGGTG+CGG + Chr5:3888588-3888607 MsG0580024362.01.T01:intron 50.0%
AGCGGAAACAGAGATCTCGT+TGG - Chr5:3888642-3888661 None:intergenic 50.0%
ATAGCAGTGATGTGGTTGCG+TGG + Chr5:3885815-3885834 MsG0580024362.01.T01:intron 50.0%
CGAGAAGTTGCAGTTGGTTG+AGG + Chr5:3888820-3888839 MsG0580024362.01.T01:CDS 50.0%
CGAGATCGGAGTCAGTTCTT+TGG + Chr5:3885277-3885296 MsG0580024362.01.T01:CDS 50.0%
CTCCAAGCAAATCCAGCAGA+TGG + Chr5:3888583-3888602 MsG0580024362.01.T01:intron 50.0%
CTTTCTCTTCGGCTTCTTGG+CGG - Chr5:3888619-3888638 None:intergenic 50.0%
GAGAAGATCGATCGAGAGAG+AGG - Chr5:3888514-3888533 None:intergenic 50.0%
GAGAGAGAGGGAAACTGCTT+CGG - Chr5:3888501-3888520 None:intergenic 50.0%
GGTCTGATTCCCACGGTAAA+TGG + Chr5:3886400-3886419 MsG0580024362.01.T01:intron 50.0%
GTCACATAGGTCCCTGATTC+AGG - Chr5:3888297-3888316 None:intergenic 50.0%
GTGAAGAAGTGGGATGTCCA+GGG - Chr5:3886932-3886951 None:intergenic 50.0%
TACAGTGGTCGTTCTCGTGT+AGG + Chr5:3885473-3885492 MsG0580024362.01.T01:intron 50.0%
TAGCAGTGATGTGGTTGCGT+GGG + Chr5:3885816-3885835 MsG0580024362.01.T01:intron 50.0%
TCGGGTGTTCCGCCTATATA+CGG + Chr5:3885568-3885587 MsG0580024362.01.T01:intron 50.0%
TGGCTTTCTCTTCGGCTTCT+TGG - Chr5:3888622-3888641 None:intergenic 50.0%
TTAACCGAGCTAAGCTCACG+GGG - Chr5:3886869-3886888 None:intergenic 50.0%
TTTCGGATGTGGCTCAGCTT+TGG + Chr5:3885391-3885410 MsG0580024362.01.T01:CDS 50.0%
TTTGCACCTCGCTGTCACAT+AGG + Chr5:3887252-3887271 MsG0580024362.01.T01:intron 50.0%
! CAAGGAGTATGACGTGTCAC+AGG + Chr5:3887382-3887401 MsG0580024362.01.T01:intron 50.0%
! CAAGGAGTATGACGTGTCAC+AGG - Chr5:3887401-3887382 None:intergenic 50.0%
! CACCATCTGCTGGATTTGCT+TGG - Chr5:3888588-3888607 None:intergenic 50.0%
! CTTTGGTGTACTGAGGGTGA+GGG + Chr5:3885204-3885223 MsG0580024362.01.T01:CDS 50.0%
! GGTGGGTGTTTTTCTTTGGG+GGG + Chr5:3885415-3885434 MsG0580024362.01.T01:intron 50.0%
! TCTTTGGTGTACTGAGGGTG+AGG + Chr5:3885203-3885222 MsG0580024362.01.T01:CDS 50.0%
! TTTGGTGTACTGAGGGTGAG+GGG + Chr5:3885205-3885224 MsG0580024362.01.T01:CDS 50.0%
!! CAAAAGGCGCCGTATATAGG+CGG - Chr5:3885580-3885599 None:intergenic 50.0%
!! GGTGGTGATGCGTATTTTGG+TGG + Chr5:3885507-3885526 MsG0580024362.01.T01:intron 50.0%
!! GTGGGTGTTTTTCTTTGGGG+GGG + Chr5:3885416-3885435 MsG0580024362.01.T01:intron 50.0%
!! TCTGCGTCGTTCATTTTGGC+TGG - Chr5:3888560-3888579 None:intergenic 50.0%
!!! CGTTCATTTTGGCTGGGAGA+GGG - Chr5:3888553-3888572 None:intergenic 50.0%
!!! CTGCGTCGTTCATTTTGGCT+GGG - Chr5:3888559-3888578 None:intergenic 50.0%
!!! GATGCGTATTTTGGTGGGTG+TGG + Chr5:3885513-3885532 MsG0580024362.01.T01:intron 50.0%
!!! GTTCATTTTGGCTGGGAGAG+GGG - Chr5:3888552-3888571 None:intergenic 50.0%
!!! TCGTTCATTTTGGCTGGGAG+AGG - Chr5:3888554-3888573 None:intergenic 50.0%
AGAGAGGGAAACTGCTTCGG+AGG - Chr5:3888498-3888517 None:intergenic 55.0%
AGGGACCTATGTGACAGCGA+GGG + Chr5:3888302-3888321 MsG0580024362.01.T01:intron 55.0%
CGGAGGGAGAGATACGAAAG+AGG - Chr5:3888481-3888500 None:intergenic 55.0%
GAATCCCCGTGAGCTTAGCT+CGG + Chr5:3886862-3886881 MsG0580024362.01.T01:intron 55.0%
GACAGCGAGGTGCAAAGTCA+GGG - Chr5:3887248-3887267 None:intergenic 55.0%
GAGAGGGAAACTGCTTCGGA+GGG - Chr5:3888497-3888516 None:intergenic 55.0%
GATCAGAGGTTGAGGAGTGG+TGG + Chr5:3885340-3885359 MsG0580024362.01.T01:CDS 55.0%
GATGATGTGGCACGCTGACA+AGG + Chr5:3887364-3887383 MsG0580024362.01.T01:intron 55.0%
GATGATGTGGCACGCTGACA+AGG - Chr5:3887383-3887364 None:intergenic 55.0%
GGTGAAGAAGTGGGATGTCC+AGG - Chr5:3886933-3886952 None:intergenic 55.0%
GTCACATAGGTCCCTGACTC+AGG + Chr5:3887265-3887284 MsG0580024362.01.T01:intron 55.0%
GTGGATCAGAGGTTGAGGAG+TGG + Chr5:3885337-3885356 MsG0580024362.01.T01:CDS 55.0%
TGACAGCGAGGTGCAAAGTC+AGG - Chr5:3887249-3887268 None:intergenic 55.0%
TGATCGGCCCACCAGACTTT+AGG - Chr5:3885248-3885267 None:intergenic 55.0%
! ATGTGGCTCAGCTTTGGTGG+TGG + Chr5:3885397-3885416 MsG0580024362.01.T01:CDS 55.0%
! TGTGGCTCAGCTTTGGTGGT+GGG + Chr5:3885398-3885417 MsG0580024362.01.T01:CDS 55.0%
! TTTGGTGGGTGTGGTCCTCA+GGG + Chr5:3885522-3885541 MsG0580024362.01.T01:intron 55.0%
!! TTTGTGTGCTGGTTGCCGGT+GGG + Chr5:3885111-3885130 MsG0580024362.01.T01:CDS 55.0%
!! TTTTGGCTGGGAGAGGGGAT+TGG - Chr5:3888547-3888566 None:intergenic 55.0%
!! TTTTGGTGGGTGTGGTCCTC+AGG + Chr5:3885521-3885540 MsG0580024362.01.T01:intron 55.0%
!!! TGAGGGGTGCCCATCTGTTT+TGG + Chr5:3885221-3885240 MsG0580024362.01.T01:CDS 55.0%
!!! TTTTGTGTGCTGGTTGCCGG+TGG + Chr5:3885110-3885129 MsG0580024362.01.T01:CDS 55.0%
CAGGGACCTATGTGACAGCG+AGG + Chr5:3888301-3888320 MsG0580024362.01.T01:intron 60.0%
CAGGGACCTATGTGACAGCG+AGG - Chr5:3888320-3888301 None:intergenic 60.0%
GGATGAACCGCACCATCTGC+TGG - Chr5:3888598-3888617 None:intergenic 60.0%
GGGACCTATGTGACAGCGAG+GGG + Chr5:3888303-3888322 MsG0580024362.01.T01:intron 60.0%
GTAGTGACGACGGGTGGTGT+TGG + Chr5:3885440-3885459 MsG0580024362.01.T01:intron 60.0%
TCTGCCCCTCGCTGTCACAT+AGG - Chr5:3888310-3888329 None:intergenic 60.0%
TTGGGGGGGAGTAGTGACGA+CGG + Chr5:3885430-3885449 MsG0580024362.01.T01:intron 60.0%
! CGGATGTGGCTCAGCTTTGG+TGG + Chr5:3885394-3885413 MsG0580024362.01.T01:CDS 60.0%
! TTGGTGGGTGTGGTCCTCAG+GGG + Chr5:3885523-3885542 MsG0580024362.01.T01:intron 60.0%
AGGGGTCAGGGTTCGAACCC+TGG + Chr5:3886912-3886931 MsG0580024362.01.T01:intron 65.0%
GACAGCGAGGGGCAGAGTCA+GGG + Chr5:3888314-3888333 MsG0580024362.01.T01:intron 65.0%
TGACAGCGAGGGGCAGAGTC+AGG + Chr5:3888313-3888332 MsG0580024362.01.T01:intron 65.0%
TGGGGGGGAGTAGTGACGAC+GGG + Chr5:3885431-3885450 MsG0580024362.01.T01:intron 65.0%
GGGGGAGTAGTGACGACGGG+TGG + Chr5:3885434-3885453 MsG0580024362.01.T01:intron 70.0%
!! TGTGCTGGTTGCCGGTGGGC+CGG + Chr5:3885115-3885134 MsG0580024362.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 3885093 3889841 3885093 ID=MsG0580024362.01;Name=MsG0580024362.01
Chr5 mRNA 3885093 3889841 3885093 ID=MsG0580024362.01.T01;Parent=MsG0580024362.01;Name=MsG0580024362.01.T01;_AED=0.50;_eAED=0.76;_QI=0|0.5|0.33|1|1|1|3|0|197
Chr5 exon 3885093 3885419 3885093 ID=MsG0580024362.01.T01:exon:1238;Parent=MsG0580024362.01.T01
Chr5 exon 3888809 3888909 3888809 ID=MsG0580024362.01.T01:exon:1239;Parent=MsG0580024362.01.T01
Chr5 exon 3889676 3889841 3889676 ID=MsG0580024362.01.T01:exon:1240;Parent=MsG0580024362.01.T01
Chr5 CDS 3885093 3885419 3885093 ID=MsG0580024362.01.T01:cds;Parent=MsG0580024362.01.T01
Chr5 CDS 3888809 3888909 3888809 ID=MsG0580024362.01.T01:cds;Parent=MsG0580024362.01.T01
Chr5 CDS 3889676 3889841 3889676 ID=MsG0580024362.01.T01:cds;Parent=MsG0580024362.01.T01
Gene Sequence

>MsG0580024362.01.T01

ATGTCGTTTGCCATACTTTTTGTGTGCTGGTTGCCGGTGGGCCGGTTAATTTTGATTCGAGATCGGGAGTTGGTGTCTAGCTGCAGTTTTGTTTGGCGTGATTTGGTATTTCTTTGGTGTACTGAGGGTGAGGGGTGCCCATCTGTTTTGGTCCTAAAGTCTGGTGGGCCGATCACTTTTGATTCGAGATCGGAGTCAGTTCTTTGGATCAATTATGGTTTTGGGCTTTTTATGGTTTCTTTCAGTGGATCAGAGGTTGAGGAGTGGTGGAAGCTGAATGATCTTTCGGATAGGAACTTTTCGGATGTGGCTCAGCTTTGGTGGTGGGAATTCAATATCGAGAAGTTGCAGTTGGTTGAGGCTGATAAGAAGAAGATCCGTCAAGAGTATGAGCGCAAAGAGAAGCAAGTTGATGTTCGTAAGAAGATTGAGTACTCTATGCAGTTGAATGCTTCACGGTTGAAGGTTCTTCAAGCCCAAGATGATGTGGTTAATAAAATGAAAGAGTCTGCCGCCAAGGAACTCTTGAATGTGAGCCGTGATCATCATGTGTACAAAAACCTTCTAAAAGATCTCGTTATTCAGGTCAGTTAA
Protein sequence

>MsG0580024362.01.T01

MSFAILFVCWLPVGRLILIRDRELVSSCSFVWRDLVFLWCTEGEGCPSVLVLKSGGPITFDSRSESVLWINYGFGLFMVSFSGSEVEEWWKLNDLSDRNFSDVAQLWWWEFNIEKLQLVEADKKKIRQEYERKEKQVDVRKKIEYSMQLNASRLKVLQAQDDVVNKMKESAAKELLNVSRDHHVYKNLLKDLVIQVS*