Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024885.01.T01 | XP_003611867.1 | 95.135 | 185 | 7 | 2 | 1 | 183 | 1 | 185 | 3.59E-125 | 360 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024885.01.T01 | Q41495 | 64.789 | 142 | 48 | 2 | 42 | 183 | 75 | 214 | 9.38E-65 | 201 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024885.01.T01 | G7KAR2 | 95.135 | 185 | 7 | 2 | 1 | 183 | 1 | 185 | 1.71e-125 | 360 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005524.01 | MsG0580024885.01 | 0.807711 | 4.422742e-50 | 2.480969e-47 |
| MsG0580024885.01 | MsG0580027925.01 | 0.801830 | 7.480578e-49 | 3.608162e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024885.01.T01 | MTR_5g018770 | 95.135 | 185 | 7 | 2 | 1 | 183 | 1 | 185 | 4.34e-129 | 360 |
| MsG0580024885.01.T01 | MTR_8g078770 | 61.326 | 181 | 61 | 3 | 3 | 183 | 1 | 172 | 2.61e-73 | 218 |
| MsG0580024885.01.T01 | MTR_5g018755 | 68.932 | 103 | 30 | 1 | 83 | 183 | 1 | 103 | 2.44e-47 | 150 |
| MsG0580024885.01.T01 | MTR_2g435490 | 33.520 | 179 | 97 | 5 | 5 | 183 | 8 | 164 | 7.39e-32 | 113 |
| MsG0580024885.01.T01 | MTR_4g128750 | 38.462 | 156 | 91 | 3 | 28 | 183 | 23 | 173 | 1.52e-31 | 112 |
| MsG0580024885.01.T01 | MTR_4g092540 | 43.357 | 143 | 72 | 6 | 42 | 183 | 25 | 159 | 1.52e-28 | 104 |
| MsG0580024885.01.T01 | MTR_2g010590 | 33.908 | 174 | 97 | 5 | 10 | 183 | 4 | 159 | 2.32e-27 | 101 |
| MsG0580024885.01.T01 | MTR_2g010600 | 32.961 | 179 | 98 | 6 | 7 | 183 | 4 | 162 | 5.05e-27 | 100 |
| MsG0580024885.01.T01 | MTR_2g010580 | 35.882 | 170 | 92 | 7 | 15 | 183 | 11 | 164 | 9.40e-27 | 100 |
| MsG0580024885.01.T01 | MTR_4g050762 | 36.879 | 141 | 81 | 5 | 45 | 183 | 28 | 162 | 6.95e-26 | 98.2 |
| MsG0580024885.01.T01 | MTR_2g012370 | 36.879 | 141 | 81 | 5 | 45 | 183 | 28 | 162 | 6.95e-26 | 98.2 |
| MsG0580024885.01.T01 | MTR_4g128770 | 32.374 | 139 | 92 | 1 | 45 | 183 | 70 | 206 | 1.63e-22 | 90.1 |
| MsG0580024885.01.T01 | MTR_2g010700 | 32.450 | 151 | 87 | 7 | 40 | 183 | 26 | 168 | 5.55e-22 | 87.8 |
| MsG0580024885.01.T01 | MTR_2g010610 | 35.971 | 139 | 61 | 5 | 45 | 183 | 28 | 138 | 2.95e-21 | 85.1 |
| MsG0580024885.01.T01 | MTR_2g010630 | 32.886 | 149 | 86 | 6 | 40 | 183 | 26 | 165 | 5.88e-21 | 85.1 |
| MsG0580024885.01.T01 | MTR_2g010650 | 33.108 | 148 | 85 | 6 | 40 | 183 | 28 | 165 | 1.36e-19 | 81.3 |
| MsG0580024885.01.T01 | MTR_1g067290 | 35.915 | 142 | 82 | 4 | 44 | 182 | 72 | 207 | 1.48e-19 | 82.4 |
| MsG0580024885.01.T01 | MTR_2g010640 | 31.818 | 176 | 103 | 8 | 12 | 183 | 1 | 163 | 6.58e-19 | 79.7 |
| MsG0580024885.01.T01 | MTR_2g010690 | 28.889 | 180 | 103 | 8 | 10 | 183 | 4 | 164 | 2.03e-15 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024885.01.T01 | AT5G66590 | 53.763 | 186 | 82 | 3 | 1 | 183 | 1 | 185 | 1.90e-62 | 191 |
| MsG0580024885.01.T01 | AT4G33720 | 41.429 | 140 | 75 | 3 | 44 | 183 | 31 | 163 | 2.73e-31 | 111 |
| MsG0580024885.01.T01 | AT5G57625 | 34.911 | 169 | 94 | 6 | 26 | 183 | 44 | 207 | 1.83e-29 | 108 |
| MsG0580024885.01.T01 | AT4G31470 | 40.000 | 140 | 78 | 4 | 44 | 183 | 52 | 185 | 4.79e-29 | 106 |
| MsG0580024885.01.T01 | AT4G25790 | 37.143 | 140 | 83 | 4 | 44 | 183 | 76 | 210 | 1.79e-28 | 105 |
| MsG0580024885.01.T01 | AT2G19990 | 37.705 | 183 | 107 | 5 | 1 | 183 | 1 | 176 | 3.40e-28 | 103 |
| MsG0580024885.01.T01 | AT3G19690 | 38.732 | 142 | 78 | 5 | 44 | 183 | 27 | 161 | 7.26e-27 | 100 |
| MsG0580024885.01.T01 | AT5G26130 | 36.806 | 144 | 78 | 5 | 44 | 183 | 32 | 166 | 4.15e-26 | 98.6 |
| MsG0580024885.01.T01 | AT2G14610 | 36.486 | 148 | 85 | 5 | 36 | 183 | 23 | 161 | 4.62e-26 | 98.2 |
| MsG0580024885.01.T01 | AT1G01310 | 38.571 | 140 | 78 | 5 | 45 | 183 | 87 | 219 | 7.27e-26 | 99.8 |
| MsG0580024885.01.T01 | AT2G14580 | 36.601 | 153 | 78 | 6 | 36 | 183 | 23 | 161 | 1.95e-25 | 96.7 |
| MsG0580024885.01.T01 | AT4G33710 | 35.570 | 149 | 89 | 4 | 36 | 183 | 24 | 166 | 4.13e-25 | 95.9 |
| MsG0580024885.01.T01 | AT3G09590 | 34.752 | 141 | 88 | 3 | 43 | 183 | 50 | 186 | 2.93e-24 | 94.4 |
| MsG0580024885.01.T01 | AT4G30320 | 36.879 | 141 | 81 | 5 | 44 | 183 | 28 | 161 | 3.97e-24 | 93.2 |
| MsG0580024885.01.T01 | AT4G25780 | 30.286 | 175 | 120 | 1 | 9 | 183 | 18 | 190 | 2.04e-23 | 92.0 |
| MsG0580024885.01.T01 | AT5G02730 | 36.691 | 139 | 84 | 4 | 45 | 183 | 59 | 193 | 7.77e-23 | 90.9 |
| MsG0580024885.01.T01 | AT1G50050 | 35.211 | 142 | 87 | 3 | 42 | 183 | 26 | 162 | 2.98e-22 | 88.2 |
| MsG0580024885.01.T01 | AT4G33730 | 36.620 | 142 | 79 | 5 | 44 | 183 | 40 | 172 | 4.31e-22 | 88.2 |
| MsG0580024885.01.T01 | AT1G50060 | 32.203 | 177 | 97 | 6 | 8 | 183 | 7 | 161 | 1.41e-21 | 86.7 |
| MsG0580024885.01.T01 | AT1G50050 | 34.586 | 133 | 82 | 3 | 42 | 174 | 26 | 153 | 5.49e-18 | 78.6 |
| MsG0580024885.01.T01 | AT4G07820 | 32.143 | 140 | 86 | 5 | 44 | 183 | 30 | 160 | 1.33e-17 | 76.3 |
| MsG0580024885.01.T01 | AT2G19970 | 32.090 | 134 | 84 | 4 | 44 | 173 | 36 | 166 | 6.19e-14 | 67.0 |
Find 50 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACATTATCACCAATCAAAA+TGG | 0.284930 | 5:-11217996 | None:intergenic |
| CCGTTACCAAAGAAACAAAA+TGG | 0.291931 | 5:+11217680 | MsG0580024885.01.T01:CDS |
| CACTCCTCCACCGCTTTGCT+TGG | 0.328916 | 5:-11217780 | None:intergenic |
| GGTGAAAGAGGCGGCGGTGC+TGG | 0.351459 | 5:-11217552 | None:intergenic |
| GGCTCTGGAATGGTGGCTGC+TGG | 0.366573 | 5:-11217531 | None:intergenic |
| GTGTGATTCTAAGAACTCTT+TGG | 0.373119 | 5:-11217580 | None:intergenic |
| AGAGGCGGCGGTGCTGGCTC+TGG | 0.382556 | 5:-11217546 | None:intergenic |
| CACCGCTTTGCTTGGCGTTA+CGG | 0.389629 | 5:-11217772 | None:intergenic |
| AATACGGTGGTAATCAGCTT+TGG | 0.400182 | 5:+11217733 | MsG0580024885.01.T01:CDS |
| GATCCACCTGGTAATGTCAT+TGG | 0.411135 | 5:+11217966 | MsG0580024885.01.T01:CDS |
| CGGCGGTGCTGGCTCTGGAA+TGG | 0.427147 | 5:-11217541 | None:intergenic |
| GGAGGAGTGGGTGAAGGAGA+AGG | 0.432515 | 5:+11217794 | MsG0580024885.01.T01:CDS |
| ATACGGTGGTAATCAGCTTT+GGG | 0.450022 | 5:+11217734 | MsG0580024885.01.T01:CDS |
| GGCTGCTGGTCTTGCAGCGG+TGG | 0.470758 | 5:-11217517 | None:intergenic |
| AACTTAACAAGCAGCAAATA+CGG | 0.475426 | 5:+11217717 | MsG0580024885.01.T01:CDS |
| TCTAAGAACTCTTTGGCTGC+TGG | 0.478483 | 5:-11217573 | None:intergenic |
| CTCTCTCCAATGACATTACC+AGG | 0.478858 | 5:-11217972 | None:intergenic |
| AACGCCAAGCAAAGCGGTGG+AGG | 0.488991 | 5:+11217776 | MsG0580024885.01.T01:CDS |
| TCTTTGGCTGCTGGTGAAAG+AGG | 0.499730 | 5:-11217564 | None:intergenic |
| AGCTTCCTTCTTACCCGTAC+AGG | 0.503016 | 5:-11217922 | None:intergenic |
| CAAGCAAAGCGGTGGAGGAG+TGG | 0.503440 | 5:+11217781 | MsG0580024885.01.T01:CDS |
| AAGCAAAGCGGTGGAGGAGT+GGG | 0.513160 | 5:+11217782 | MsG0580024885.01.T01:CDS |
| AGCTACCTGTACGGGTAAGA+AGG | 0.523085 | 5:+11217917 | MsG0580024885.01.T01:CDS |
| CGGTGCTGGCTCTGGAATGG+TGG | 0.525884 | 5:-11217538 | None:intergenic |
| GCTTGGCGTTACGGCTGCTG+CGG | 0.526234 | 5:-11217763 | None:intergenic |
| CGGTGGACGAGACAAAGAGC+AGG | 0.547518 | 5:-11217500 | None:intergenic |
| AGCTTCTCGCTCCATTGAAG+TGG | 0.547634 | 5:-11217633 | None:intergenic |
| TAGGTGTTGAGCCACTTCAA+TGG | 0.550994 | 5:+11217622 | MsG0580024885.01.T01:CDS |
| GGTGGTAATCAGCTTTGGGC+GGG | 0.552262 | 5:+11217738 | MsG0580024885.01.T01:CDS |
| TGTTCACAAGCTACCTGTAC+GGG | 0.553174 | 5:+11217909 | MsG0580024885.01.T01:CDS |
| GCTGCTGGTGAAAGAGGCGG+CGG | 0.563476 | 5:-11217558 | None:intergenic |
| AGTGTGGCTAGAGAGAAGAG+AGG | 0.565171 | 5:-11217474 | None:intergenic |
| CGTTACCAAAGAAACAAAAT+GGG | 0.569158 | 5:+11217681 | MsG0580024885.01.T01:CDS |
| GTGTTCACAAGCTACCTGTA+CGG | 0.570401 | 5:+11217908 | MsG0580024885.01.T01:CDS |
| AGCGGTGGAGGAGTGGGTGA+AGG | 0.573564 | 5:+11217788 | MsG0580024885.01.T01:CDS |
| CGGTGGTAATCAGCTTTGGG+CGG | 0.593924 | 5:+11217737 | MsG0580024885.01.T01:CDS |
| ATTTGTTTCTATGATCCACC+TGG | 0.608612 | 5:+11217954 | MsG0580024885.01.T01:CDS |
| GACAAAGAGCAGGGATAGTG+TGG | 0.609020 | 5:-11217490 | None:intergenic |
| TGGAAGAAATCAGTACAACT+TGG | 0.618139 | 5:+11217885 | MsG0580024885.01.T01:CDS |
| GGTGGCTGCTGGTCTTGCAG+CGG | 0.618405 | 5:-11217520 | None:intergenic |
| TTGGCTGCTGGTGAAAGAGG+CGG | 0.629160 | 5:-11217561 | None:intergenic |
| GTGTTTATACGCAAGTTGTG+TGG | 0.629612 | 5:+11217865 | MsG0580024885.01.T01:CDS |
| GGAAGAAATCAGTACAACTT+GGG | 0.648064 | 5:+11217886 | MsG0580024885.01.T01:CDS |
| CGTAACGCCAAGCAAAGCGG+TGG | 0.663476 | 5:+11217773 | MsG0580024885.01.T01:CDS |
| AACAAAGCAAGAGCTGAAGT+AGG | 0.671065 | 5:+11217603 | MsG0580024885.01.T01:CDS |
| AGCCGTAACGCCAAGCAAAG+CGG | 0.678151 | 5:+11217770 | MsG0580024885.01.T01:CDS |
| GGTGGACGAGACAAAGAGCA+GGG | 0.679486 | 5:-11217499 | None:intergenic |
| TGTGTTGCGAATCATGAGTG+TGG | 0.721846 | 5:+11217843 | MsG0580024885.01.T01:CDS |
| TTAACAAGCAGCAAATACGG+TGG | 0.752196 | 5:+11217720 | MsG0580024885.01.T01:CDS |
| TCTCCAATGACATTACCAGG+TGG | 0.762082 | 5:-11217969 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACTTAACAAGCAGCAAATA+CGG | + | Chr5:11217717-11217736 | MsG0580024885.01.T01:CDS | 30.0% | |
| CGTTACCAAAGAAACAAAAT+GGG | + | Chr5:11217681-11217700 | MsG0580024885.01.T01:CDS | 30.0% | |
| !! | CCATTTTGTTTCTTTGGTAA+CGG | - | Chr5:11217683-11217702 | None:intergenic | 30.0% |
| CCGTTACCAAAGAAACAAAA+TGG | + | Chr5:11217680-11217699 | MsG0580024885.01.T01:CDS | 35.0% | |
| GGAAGAAATCAGTACAACTT+GGG | + | Chr5:11217886-11217905 | MsG0580024885.01.T01:CDS | 35.0% | |
| TGGAAGAAATCAGTACAACT+TGG | + | Chr5:11217885-11217904 | MsG0580024885.01.T01:CDS | 35.0% | |
| ! | GTGTGATTCTAAGAACTCTT+TGG | - | Chr5:11217583-11217602 | None:intergenic | 35.0% |
| !! | ATTTGTTTCTATGATCCACC+TGG | + | Chr5:11217954-11217973 | MsG0580024885.01.T01:CDS | 35.0% |
| AACAAAGCAAGAGCTGAAGT+AGG | + | Chr5:11217603-11217622 | MsG0580024885.01.T01:CDS | 40.0% | |
| AATACGGTGGTAATCAGCTT+TGG | + | Chr5:11217733-11217752 | MsG0580024885.01.T01:CDS | 40.0% | |
| ATACGGTGGTAATCAGCTTT+GGG | + | Chr5:11217734-11217753 | MsG0580024885.01.T01:CDS | 40.0% | |
| GTGTTTATACGCAAGTTGTG+TGG | + | Chr5:11217865-11217884 | MsG0580024885.01.T01:CDS | 40.0% | |
| TTAACAAGCAGCAAATACGG+TGG | + | Chr5:11217720-11217739 | MsG0580024885.01.T01:CDS | 40.0% | |
| !! | CGCAACCCATTTTGTTTCTT+TGG | - | Chr5:11217689-11217708 | None:intergenic | 40.0% |
| CTCTCTCCAATGACATTACC+AGG | - | Chr5:11217975-11217994 | None:intergenic | 45.0% | |
| GTGTTCACAAGCTACCTGTA+CGG | + | Chr5:11217908-11217927 | MsG0580024885.01.T01:CDS | 45.0% | |
| TCTAAGAACTCTTTGGCTGC+TGG | - | Chr5:11217576-11217595 | None:intergenic | 45.0% | |
| TCTCCAATGACATTACCAGG+TGG | - | Chr5:11217972-11217991 | None:intergenic | 45.0% | |
| TGTGTTGCGAATCATGAGTG+TGG | + | Chr5:11217843-11217862 | MsG0580024885.01.T01:CDS | 45.0% | |
| TGTTCACAAGCTACCTGTAC+GGG | + | Chr5:11217909-11217928 | MsG0580024885.01.T01:CDS | 45.0% | |
| ! | GATCCACCTGGTAATGTCAT+TGG | + | Chr5:11217966-11217985 | MsG0580024885.01.T01:CDS | 45.0% |
| !! | TAGGTGTTGAGCCACTTCAA+TGG | + | Chr5:11217622-11217641 | MsG0580024885.01.T01:CDS | 45.0% |
| AGCTACCTGTACGGGTAAGA+AGG | + | Chr5:11217917-11217936 | MsG0580024885.01.T01:CDS | 50.0% | |
| AGCTTCCTTCTTACCCGTAC+AGG | - | Chr5:11217925-11217944 | None:intergenic | 50.0% | |
| AGCTTCTCGCTCCATTGAAG+TGG | - | Chr5:11217636-11217655 | None:intergenic | 50.0% | |
| AGTGTGGCTAGAGAGAAGAG+AGG | - | Chr5:11217477-11217496 | None:intergenic | 50.0% | |
| GACAAAGAGCAGGGATAGTG+TGG | - | Chr5:11217493-11217512 | None:intergenic | 50.0% | |
| TCTTTGGCTGCTGGTGAAAG+AGG | - | Chr5:11217567-11217586 | None:intergenic | 50.0% | |
| AAGCAAAGCGGTGGAGGAGT+GGG | + | Chr5:11217782-11217801 | MsG0580024885.01.T01:CDS | 55.0% | |
| AGCCGTAACGCCAAGCAAAG+CGG | + | Chr5:11217770-11217789 | MsG0580024885.01.T01:CDS | 55.0% | |
| CACCGCTTTGCTTGGCGTTA+CGG | - | Chr5:11217775-11217794 | None:intergenic | 55.0% | |
| GGTGGACGAGACAAAGAGCA+GGG | - | Chr5:11217502-11217521 | None:intergenic | 55.0% | |
| TTGGCTGCTGGTGAAAGAGG+CGG | - | Chr5:11217564-11217583 | None:intergenic | 55.0% | |
| !! | CGGTGGTAATCAGCTTTGGG+CGG | + | Chr5:11217737-11217756 | MsG0580024885.01.T01:CDS | 55.0% |
| !! | GGTGGTAATCAGCTTTGGGC+GGG | + | Chr5:11217738-11217757 | MsG0580024885.01.T01:CDS | 55.0% |
| AACGCCAAGCAAAGCGGTGG+AGG | + | Chr5:11217776-11217795 | MsG0580024885.01.T01:CDS | 60.0% | |
| CAAGCAAAGCGGTGGAGGAG+TGG | + | Chr5:11217781-11217800 | MsG0580024885.01.T01:CDS | 60.0% | |
| CACTCCTCCACCGCTTTGCT+TGG | - | Chr5:11217783-11217802 | None:intergenic | 60.0% | |
| CGGTGGACGAGACAAAGAGC+AGG | - | Chr5:11217503-11217522 | None:intergenic | 60.0% | |
| CGTAACGCCAAGCAAAGCGG+TGG | + | Chr5:11217773-11217792 | MsG0580024885.01.T01:CDS | 60.0% | |
| GGAGGAGTGGGTGAAGGAGA+AGG | + | Chr5:11217794-11217813 | MsG0580024885.01.T01:CDS | 60.0% | |
| AGCGGTGGAGGAGTGGGTGA+AGG | + | Chr5:11217788-11217807 | MsG0580024885.01.T01:CDS | 65.0% | |
| GCTGCTGGTGAAAGAGGCGG+CGG | - | Chr5:11217561-11217580 | None:intergenic | 65.0% | |
| GCTTGGCGTTACGGCTGCTG+CGG | - | Chr5:11217766-11217785 | None:intergenic | 65.0% | |
| !! | CGGTGCTGGCTCTGGAATGG+TGG | - | Chr5:11217541-11217560 | None:intergenic | 65.0% |
| !! | GGCTCTGGAATGGTGGCTGC+TGG | - | Chr5:11217534-11217553 | None:intergenic | 65.0% |
| !! | GGTGGCTGCTGGTCTTGCAG+CGG | - | Chr5:11217523-11217542 | None:intergenic | 65.0% |
| GGTGAAAGAGGCGGCGGTGC+TGG | - | Chr5:11217555-11217574 | None:intergenic | 70.0% | |
| !! | CGGCGGTGCTGGCTCTGGAA+TGG | - | Chr5:11217544-11217563 | None:intergenic | 70.0% |
| !! | GGCTGCTGGTCTTGCAGCGG+TGG | - | Chr5:11217520-11217539 | None:intergenic | 70.0% |
| !! | AGAGGCGGCGGTGCTGGCTC+TGG | - | Chr5:11217549-11217568 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 11217453 | 11218004 | 11217453 | ID=MsG0580024885.01;Name=MsG0580024885.01 |
| Chr5 | mRNA | 11217453 | 11218004 | 11217453 | ID=MsG0580024885.01.T01;Parent=MsG0580024885.01;Name=MsG0580024885.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|183 |
| Chr5 | exon | 11217453 | 11218004 | 11217453 | ID=MsG0580024885.01.T01:exon:26667;Parent=MsG0580024885.01.T01 |
| Chr5 | CDS | 11217453 | 11218004 | 11217453 | ID=MsG0580024885.01.T01:cds;Parent=MsG0580024885.01.T01 |
| Gene Sequence |
| Protein sequence |
