AlfalfaGEDB | MsG0580025336.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025336.01

KEGG: K17893
GO: GO:0009916 GO:0055114
PFAM: PF01786

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025336.01.T01	XP_027360033.1	96.875	64	2	0	1	64	164	227	1.37E-36	134

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025336.01.T01	Q39219	95.312	64	3	0	1	64	230	293	2.66E-38	132

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025336.01.T01	A0A2H4KDH8	96.875	64	2	0	1	64	211	274	5.18e-36	132
MsG0580025336.01.T01	A0A654FAW8	95.312	64	3	0	1	64	230	293	1.02e-35	132

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0580025336.01	MsG0580025338.01	0.865331	6.382562e-65	2.068535e-61
MsG0580025336.01	MsG0580028560.01	0.800901	1.159390e-48	5.462565e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025336.01.T01	MTR_5g026620	96.875	64	2	0	1	64	206	269	1.39e-39	132
MsG0580025336.01.T01	MTR_5g026620	95.385	65	3	0	1	65	138	202	4.02e-39	129
MsG0580025336.01.T01	MTR_4g094222	70.312	64	19	0	1	64	221	284	1.77e-27	101
MsG0580025336.01.T01	MTR_5g070680	59.375	64	26	0	1	64	199	262	1.51e-21	85.5
MsG0580025336.01.T01	MTR_5g070870	57.812	64	27	0	1	64	200	263	6.62e-16	70.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025336.01.T01	AT3G22370	95.312	64	3	0	1	64	230	293	2.72e-39	132
MsG0580025336.01.T01	AT3G22360	89.231	65	7	0	1	65	201	265	5.37e-36	123
MsG0580025336.01.T01	AT3G27620	87.500	64	8	0	1	64	205	268	8.60e-36	122
MsG0580025336.01.T01	AT5G64210	68.750	64	20	0	1	64	229	292	1.27e-28	104
MsG0580025336.01.T01	AT1G32350	67.188	64	21	0	1	64	194	257	6.02e-28	102
MsG0580025336.01.T01	AT1G32350	67.188	64	21	0	1	64	194	257	9.23e-28	101

Find 18 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCATACGGTGTGCAAATT+TGG	0.112313	5:+17107879	None:intergenic
TTCTTCAATGCTTATTTCTT+AGG	0.253052	5:-17107916	MsG0580025336.01.T01:CDS
TTTCTCAAGGAACTTGATAA+AGG	0.335708	5:-17107817	MsG0580025336.01.T01:CDS
ACCATACGGTGTGCAAATTT+GGG	0.381391	5:+17107880	None:intergenic
TGCACTTGTCATAACTGTCC+AGG	0.458104	5:-17107944	MsG0580025336.01.T01:CDS
TAATAGTCAATAGCAATAGC+TGG	0.464270	5:+17107774	None:intergenic
GTCAATAGCAATAGCTGGGC+AGG	0.475463	5:+17107779	None:intergenic
TCCAATGACTTTCATGGAAG+TGG	0.475736	5:-17107989	None:intergenic
ACCCAAATTTGCACACCGTA+TGG	0.511597	5:-17107881	MsG0580025336.01.T01:CDS
AGTGCACACTCATACCACTT+TGG	0.562854	5:+17107961	None:intergenic
AAATTTGCACACCGTATGGT+TGG	0.563076	5:-17107877	MsG0580025336.01.T01:CDS
CTCATACACTGAGTTTCTCA+AGG	0.566793	5:-17107830	MsG0580025336.01.T01:CDS
GCACTTGTCATAACTGTCCA+GGG	0.578082	5:-17107943	MsG0580025336.01.T01:CDS
AGTGAATAGCTTCTTCCTCA+AGG	0.587319	5:+17107851	None:intergenic
AATAGTCAATAGCAATAGCT+GGG	0.594406	5:+17107775	None:intergenic
CCTCAAGGTAACCAACCATA+CGG	0.624191	5:+17107866	None:intergenic
TGGAAGTGGCAAAGCCAAAG+TGG	0.631195	5:-17107975	MsG0580025336.01.T01:CDS
CCGTATGGTTGGTTACCTTG+AGG	0.649479	5:-17107866	MsG0580025336.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTCTTCAATGCTTATTTCTT+AGG	-	Chr5:17107840-17107859	MsG0580025336.01.T01:CDS	25.0%
	TTTCTCAAGGAACTTGATAA+AGG	-	Chr5:17107939-17107958	MsG0580025336.01.T01:CDS	30.0%
!!	AAGCATTGAAGAAAAAACCC+TGG	+	Chr5:17107833-17107852	None:intergenic	35.0%
	AAATTTGCACACCGTATGGT+TGG	-	Chr5:17107879-17107898	MsG0580025336.01.T01:CDS	40.0%
	AACCATACGGTGTGCAAATT+TGG	+	Chr5:17107880-17107899	None:intergenic	40.0%
	ACCATACGGTGTGCAAATTT+GGG	+	Chr5:17107879-17107898	None:intergenic	40.0%
	AGTGAATAGCTTCTTCCTCA+AGG	+	Chr5:17107908-17107927	None:intergenic	40.0%
	CTCATACACTGAGTTTCTCA+AGG	-	Chr5:17107926-17107945	MsG0580025336.01.T01:CDS	40.0%
	ACCCAAATTTGCACACCGTA+TGG	-	Chr5:17107875-17107894	MsG0580025336.01.T01:CDS	45.0%
	AGTGCACACTCATACCACTT+TGG	+	Chr5:17107798-17107817	None:intergenic	45.0%
	CCTCAAGGTAACCAACCATA+CGG	+	Chr5:17107893-17107912	None:intergenic	45.0%
	GCACTTGTCATAACTGTCCA+GGG	-	Chr5:17107813-17107832	MsG0580025336.01.T01:CDS	45.0%
	TGCACTTGTCATAACTGTCC+AGG	-	Chr5:17107812-17107831	MsG0580025336.01.T01:CDS	45.0%
	TGGAAGTGGCAAAGCCAAAG+TGG	-	Chr5:17107781-17107800	MsG0580025336.01.T01:CDS	50.0%
!	CCGTATGGTTGGTTACCTTG+AGG	-	Chr5:17107890-17107909	MsG0580025336.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	17107780	17107998	17107780	ID=MsG0580025336.01;Name=MsG0580025336.01
Chr5	mRNA	17107780	17107998	17107780	ID=MsG0580025336.01.T01;Parent=MsG0580025336.01;Name=MsG0580025336.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|72
Chr5	exon	17107780	17107998	17107780	ID=MsG0580025336.01.T01:exon:18990;Parent=MsG0580025336.01.T01
Chr5	CDS	17107780	17107998	17107780	ID=MsG0580025336.01.T01:cds;Parent=MsG0580025336.01.T01

Gene Sequence	>MsG0580025336.01.T01 ATGGAAGTGGCAAAGCCAAAGTGGTATGAGTGTGCACTTGTCATAACTGTCCAGGGTTTTTTCTTCAATGCTTATTTCTTAGGCTATTTGCTCTCACCCAAATTTGCACACCGTATGGTTGGTTACCTTGAGGAAGAAGCTATTCACTCATACACTGAGTTTCTCAAGGAACTTGATAAAGGCAACATAGAAACGTTCCTGCCCAGCTATTGCTATTGA
Protein sequence	>MsG0580025336.01.T01 MEVAKPKWYECALVITVQGFFFNAYFLGYLLSPKFAHRMVGYLEEEAIHSYTEFLKELDKGNIETFLPSYCY*

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