Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025537.01.T01 | XP_024634710.1 | 72.5 | 120 | 33 | 0 | 1 | 120 | 190 | 309 | 4.06E-55 | 186 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025537.01.T01 | A0A396IKN1 | 70.000 | 120 | 36 | 0 | 1 | 120 | 396 | 515 | 7.02e-50 | 176 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0580025537.01 | MsG0280010449.01 | PPI |
| MsG0580025537.01 | MsG0780041116.01 | PPI |
| MsG0280009322.01 | MsG0580025537.01 | PPI |
| MsG0780040274.01 | MsG0580025537.01 | PPI |
| MsG0580025537.01 | MsG0780041116.01 | PPI |
| MsG0580025537.01 | MsG0380013578.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025537.01.T01 | MTR_7g013520 | 72.277 | 101 | 28 | 0 | 1 | 101 | 49 | 149 | 6.31e-48 | 152 |
| MsG0580025537.01.T01 | MTR_6g089560 | 60.185 | 108 | 43 | 0 | 1 | 108 | 95 | 202 | 2.69e-45 | 147 |
| MsG0580025537.01.T01 | MTR_1g018300 | 58.394 | 137 | 51 | 1 | 1 | 137 | 42 | 172 | 1.68e-44 | 146 |
| MsG0580025537.01.T01 | MTR_2g047100 | 55.172 | 116 | 37 | 1 | 5 | 120 | 1 | 101 | 2.08e-38 | 125 |
| MsG0580025537.01.T01 | MTR_8g099580 | 51.220 | 123 | 60 | 0 | 1 | 123 | 185 | 307 | 5.76e-38 | 130 |
| MsG0580025537.01.T01 | MTR_1g017840 | 60.577 | 104 | 41 | 0 | 2 | 105 | 58 | 161 | 4.17e-36 | 122 |
| MsG0580025537.01.T01 | MTR_4g029360 | 50.420 | 119 | 59 | 0 | 1 | 119 | 106 | 224 | 3.40e-34 | 119 |
| MsG0580025537.01.T01 | MTR_6g046670 | 72.000 | 75 | 21 | 0 | 1 | 75 | 102 | 176 | 1.73e-33 | 116 |
| MsG0580025537.01.T01 | MTR_1g088650 | 50.427 | 117 | 58 | 0 | 3 | 119 | 67 | 183 | 1.08e-31 | 111 |
| MsG0580025537.01.T01 | MTR_6g016265 | 46.296 | 108 | 58 | 0 | 1 | 108 | 18 | 125 | 4.19e-31 | 108 |
| MsG0580025537.01.T01 | MTR_8g098925 | 49.580 | 119 | 46 | 1 | 1 | 119 | 58 | 162 | 5.12e-31 | 108 |
| MsG0580025537.01.T01 | MTR_1g054905 | 73.846 | 65 | 17 | 0 | 1 | 65 | 80 | 144 | 3.12e-29 | 104 |
| MsG0580025537.01.T01 | MTR_1g081930 | 44.545 | 110 | 61 | 0 | 1 | 110 | 17 | 126 | 4.62e-29 | 103 |
| MsG0580025537.01.T01 | MTR_1g034430 | 45.455 | 110 | 60 | 0 | 1 | 110 | 74 | 183 | 7.73e-29 | 104 |
| MsG0580025537.01.T01 | MTR_3g116210 | 43.363 | 113 | 64 | 0 | 1 | 113 | 76 | 188 | 1.09e-27 | 101 |
| MsG0580025537.01.T01 | MTR_5g049680 | 47.959 | 98 | 50 | 1 | 1 | 97 | 10 | 107 | 1.45e-27 | 98.6 |
| MsG0580025537.01.T01 | MTR_5g037920 | 45.455 | 121 | 60 | 3 | 2 | 119 | 78 | 195 | 3.70e-26 | 98.6 |
| MsG0580025537.01.T01 | MTR_6g045427 | 46.429 | 112 | 32 | 1 | 1 | 112 | 152 | 235 | 1.14e-25 | 97.4 |
| MsG0580025537.01.T01 | MTR_8g022750 | 42.017 | 119 | 52 | 1 | 1 | 119 | 184 | 285 | 1.15e-25 | 98.6 |
| MsG0580025537.01.T01 | MTR_8g031800 | 48.958 | 96 | 49 | 0 | 2 | 97 | 11 | 106 | 1.80e-25 | 93.6 |
| MsG0580025537.01.T01 | MTR_1g069475 | 43.689 | 103 | 58 | 0 | 1 | 103 | 143 | 245 | 2.79e-25 | 96.7 |
| MsG0580025537.01.T01 | MTR_6g453270 | 46.000 | 100 | 54 | 0 | 1 | 100 | 279 | 378 | 4.60e-25 | 98.6 |
| MsG0580025537.01.T01 | MTR_5g083200 | 38.793 | 116 | 71 | 0 | 1 | 116 | 77 | 192 | 1.08e-24 | 94.0 |
| MsG0580025537.01.T01 | MTR_0002s1220 | 38.793 | 116 | 71 | 0 | 1 | 116 | 77 | 192 | 1.08e-24 | 94.0 |
| MsG0580025537.01.T01 | MTR_0093s0070 | 58.025 | 81 | 34 | 0 | 24 | 104 | 125 | 205 | 1.22e-24 | 94.0 |
| MsG0580025537.01.T01 | MTR_5g085210 | 43.810 | 105 | 59 | 0 | 1 | 105 | 98 | 202 | 1.03e-23 | 92.4 |
| MsG0580025537.01.T01 | MTR_3g111390 | 37.069 | 116 | 73 | 0 | 1 | 116 | 77 | 192 | 1.27e-23 | 91.3 |
| MsG0580025537.01.T01 | MTR_1g070820 | 37.069 | 116 | 73 | 0 | 1 | 116 | 77 | 192 | 1.27e-23 | 91.3 |
| MsG0580025537.01.T01 | MTR_1g045340 | 38.261 | 115 | 71 | 0 | 2 | 116 | 64 | 178 | 8.23e-23 | 88.6 |
| MsG0580025537.01.T01 | MTR_0015s0120 | 49.438 | 89 | 45 | 0 | 7 | 95 | 278 | 366 | 1.45e-22 | 91.3 |
| MsG0580025537.01.T01 | MTR_7g078610 | 60.938 | 64 | 25 | 0 | 1 | 64 | 30 | 93 | 1.97e-22 | 85.1 |
| MsG0580025537.01.T01 | MTR_5g034450 | 50.633 | 79 | 39 | 0 | 7 | 85 | 34 | 112 | 2.11e-22 | 85.9 |
| MsG0580025537.01.T01 | MTR_5g036320 | 45.882 | 85 | 46 | 0 | 1 | 85 | 58 | 142 | 1.05e-21 | 84.7 |
| MsG0580025537.01.T01 | MTR_8g036780 | 47.500 | 80 | 42 | 0 | 1 | 80 | 13 | 92 | 1.26e-21 | 83.6 |
| MsG0580025537.01.T01 | MTR_7g063280 | 36.364 | 99 | 63 | 0 | 22 | 120 | 72 | 170 | 3.33e-21 | 84.3 |
| MsG0580025537.01.T01 | MTR_2g027020 | 39.167 | 120 | 67 | 3 | 1 | 119 | 7 | 121 | 4.95e-21 | 84.3 |
| MsG0580025537.01.T01 | MTR_2g059470 | 47.561 | 82 | 43 | 0 | 24 | 105 | 2 | 83 | 5.24e-21 | 81.3 |
| MsG0580025537.01.T01 | MTR_1g074090 | 45.455 | 88 | 48 | 0 | 1 | 88 | 60 | 147 | 5.30e-21 | 83.2 |
| MsG0580025537.01.T01 | MTR_2g017550 | 50.000 | 78 | 39 | 0 | 1 | 78 | 87 | 164 | 1.24e-20 | 82.4 |
| MsG0580025537.01.T01 | MTR_2g055490 | 62.712 | 59 | 22 | 0 | 27 | 85 | 12 | 70 | 3.15e-20 | 80.1 |
| MsG0580025537.01.T01 | MTR_1g022215 | 50.725 | 69 | 34 | 0 | 10 | 78 | 2 | 70 | 3.59e-20 | 79.3 |
| MsG0580025537.01.T01 | MTR_8g038340 | 46.835 | 79 | 42 | 0 | 1 | 79 | 168 | 246 | 4.09e-20 | 83.2 |
| MsG0580025537.01.T01 | MTR_4g008270 | 46.988 | 83 | 44 | 0 | 23 | 105 | 11 | 93 | 9.58e-20 | 78.2 |
| MsG0580025537.01.T01 | MTR_8g028785 | 34.646 | 127 | 59 | 1 | 1 | 127 | 261 | 363 | 1.08e-18 | 80.9 |
| MsG0580025537.01.T01 | MTR_1g050488 | 44.444 | 81 | 45 | 0 | 7 | 87 | 83 | 163 | 1.26e-18 | 77.4 |
| MsG0580025537.01.T01 | MTR_1g099430 | 48.052 | 77 | 40 | 0 | 1 | 77 | 104 | 180 | 4.34e-18 | 77.0 |
| MsG0580025537.01.T01 | MTR_5g075610 | 43.478 | 92 | 50 | 1 | 28 | 119 | 1 | 90 | 1.12e-17 | 73.2 |
| MsG0580025537.01.T01 | MTR_8g079540 | 42.105 | 95 | 54 | 1 | 1 | 94 | 27 | 121 | 1.53e-17 | 73.6 |
| MsG0580025537.01.T01 | MTR_8g069135 | 66.000 | 50 | 17 | 0 | 1 | 50 | 168 | 217 | 1.06e-16 | 75.1 |
| MsG0580025537.01.T01 | MTR_4g094272 | 47.826 | 69 | 35 | 1 | 1 | 69 | 73 | 140 | 4.71e-16 | 70.1 |
| MsG0580025537.01.T01 | MTR_6g033225 | 41.860 | 86 | 50 | 0 | 5 | 90 | 53 | 138 | 5.28e-16 | 70.1 |
| MsG0580025537.01.T01 | MTR_6g034915 | 31.667 | 120 | 52 | 1 | 7 | 96 | 10 | 129 | 8.05e-16 | 70.5 |
| MsG0580025537.01.T01 | MTR_6g016355 | 40.000 | 80 | 48 | 0 | 5 | 84 | 99 | 178 | 8.25e-16 | 70.5 |
| MsG0580025537.01.T01 | MTR_1g062840 | 46.250 | 80 | 41 | 1 | 50 | 127 | 8 | 87 | 9.15e-16 | 68.2 |
| MsG0580025537.01.T01 | MTR_5g068115 | 53.846 | 52 | 24 | 0 | 1 | 52 | 13 | 64 | 3.86e-15 | 65.9 |
| MsG0580025537.01.T01 | MTR_4g046027 | 50.794 | 63 | 31 | 0 | 2 | 64 | 107 | 169 | 2.00e-14 | 66.6 |
| MsG0580025537.01.T01 | MTR_1964s0010 | 62.222 | 45 | 17 | 0 | 1 | 45 | 48 | 92 | 2.56e-14 | 64.7 |
| MsG0580025537.01.T01 | MTR_3g462490 | 51.613 | 62 | 30 | 0 | 1 | 62 | 80 | 141 | 2.67e-14 | 68.6 |
| MsG0580025537.01.T01 | MTR_3g010950 | 30.534 | 131 | 61 | 3 | 1 | 131 | 5 | 105 | 5.74e-14 | 64.7 |
| MsG0580025537.01.T01 | MTR_8g031080 | 48.333 | 60 | 31 | 0 | 1 | 60 | 268 | 327 | 7.48e-14 | 67.4 |
| MsG0580025537.01.T01 | MTR_8g059135 | 36.957 | 92 | 58 | 0 | 25 | 116 | 31 | 122 | 1.03e-13 | 63.5 |
| MsG0580025537.01.T01 | MTR_4g094370 | 43.750 | 64 | 36 | 0 | 1 | 64 | 14 | 77 | 1.65e-13 | 62.0 |
| MsG0580025537.01.T01 | MTR_3g465840 | 43.056 | 72 | 40 | 1 | 56 | 126 | 9 | 80 | 2.15e-13 | 63.5 |
| MsG0580025537.01.T01 | MTR_1g074240 | 42.647 | 68 | 39 | 0 | 24 | 91 | 2 | 69 | 3.72e-13 | 60.8 |
| MsG0580025537.01.T01 | MTR_3g064060 | 54.762 | 42 | 19 | 0 | 1 | 42 | 16 | 57 | 5.01e-13 | 60.1 |
| MsG0580025537.01.T01 | MTR_1g103580 | 31.933 | 119 | 29 | 2 | 1 | 119 | 139 | 205 | 3.97e-12 | 62.0 |
| MsG0580025537.01.T01 | MTR_1g109000 | 35.802 | 81 | 52 | 0 | 1 | 81 | 5 | 85 | 5.91e-12 | 58.2 |
| MsG0580025537.01.T01 | MTR_2g102247 | 56.098 | 41 | 18 | 0 | 1 | 41 | 108 | 148 | 1.46e-11 | 58.5 |
| MsG0580025537.01.T01 | MTR_7g006380 | 27.500 | 120 | 60 | 2 | 1 | 120 | 45 | 137 | 3.26e-11 | 58.2 |
| MsG0580025537.01.T01 | MTR_3g102000 | 54.762 | 42 | 19 | 0 | 1 | 42 | 182 | 223 | 3.27e-11 | 58.9 |
| MsG0580025537.01.T01 | MTR_5g069700 | 39.394 | 66 | 40 | 0 | 48 | 113 | 7 | 72 | 3.76e-11 | 55.8 |
| MsG0580025537.01.T01 | MTR_5g042170 | 47.619 | 63 | 33 | 0 | 1 | 63 | 29 | 91 | 6.78e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCTCAGGTACCTTTGTTT+TGG | 0.285227 | 5:-20206225 | MsG0580025537.01.T01:CDS |
| AGAAATAATGCTGCCAAATT+CGG | 0.287415 | 5:+20206037 | None:intergenic |
| AGGGACGACACATCCGAATT+TGG | 0.338913 | 5:-20206050 | MsG0580025537.01.T01:CDS |
| ATTGTGGCAACCTCCTTTAC+CGG | 0.344453 | 5:-20206324 | None:intergenic |
| CCCCTCAACCGCACGGGTAT+TGG | 0.368712 | 5:-20206263 | MsG0580025537.01.T01:CDS |
| CTGATGTAGTTGAAGCGTTT+CGG | 0.379838 | 5:-20206169 | MsG0580025537.01.T01:CDS |
| TGCCAATACCCGTGCGGTTG+AGG | 0.397279 | 5:+20206261 | None:intergenic |
| GCCAATACCCGTGCGGTTGA+GGG | 0.463374 | 5:+20206262 | None:intergenic |
| ATTTGTGTCCGTGATTCCTC+AGG | 0.485004 | 5:-20206239 | MsG0580025537.01.T01:CDS |
| CATTTATATCGACCCGGTAA+AGG | 0.491132 | 5:+20206311 | None:intergenic |
| TGATAGCGTGGACTTTGAAA+CGG | 0.507014 | 5:-20206108 | MsG0580025537.01.T01:CDS |
| CTCTTCTCCCCTCAACCGCA+CGG | 0.507038 | 5:-20206270 | MsG0580025537.01.T01:CDS |
| TTGTGGCAACCTCCTTTACC+GGG | 0.553980 | 5:-20206323 | MsG0580025537.01.T01:CDS |
| CAAAGGTACCTGAGGAATCA+CGG | 0.570665 | 5:+20206231 | None:intergenic |
| TCTTCTCCCCTCAACCGCAC+GGG | 0.572140 | 5:-20206269 | MsG0580025537.01.T01:CDS |
| GGTTGCACGCTGCATTACAA+TGG | 0.584973 | 5:-20206148 | MsG0580025537.01.T01:CDS |
| AAGAGCATGAGCAGCCTCGT+TGG | 0.586252 | 5:+20205951 | None:intergenic |
| CCAATACCCGTGCGGTTGAG+GGG | 0.589409 | 5:+20206263 | None:intergenic |
| TTATATCGACCCGGTAAAGG+AGG | 0.609440 | 5:+20206314 | None:intergenic |
| TGATATGCAGTTTGATAGCG+TGG | 0.623573 | 5:-20206120 | MsG0580025537.01.T01:CDS |
| AGCCAAAACAAAGGTACCTG+AGG | 0.630672 | 5:+20206223 | None:intergenic |
| ATGTTGCATTTATATCGACC+CGG | 0.641396 | 5:+20206305 | None:intergenic |
| CACAACTTTAGCCAAAACAA+AGG | 0.653323 | 5:+20206214 | None:intergenic |
| GGCGGGACTAGCTAGTAACA+TGG | 0.681637 | 5:+20205918 | None:intergenic |
| CACAAATGCCAATACCCGTG+CGG | 0.747333 | 5:+20206255 | None:intergenic |
| GTTGAGGGGAGAAGAGAACG+CGG | 0.775828 | 5:+20206277 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGAAATAATGCTGCCAAATT+CGG | + | Chr5:20206215-20206234 | None:intergenic | 30.0% | |
| ATGTTGCATTTATATCGACC+CGG | + | Chr5:20205947-20205966 | None:intergenic | 35.0% | |
| CACAACTTTAGCCAAAACAA+AGG | + | Chr5:20206038-20206057 | None:intergenic | 35.0% | |
| TAAAAAACTCCACCTAGAGT+TGG | + | Chr5:20206269-20206288 | None:intergenic | 35.0% | |
| ! | TGATGCTTTTCTTTCTGACA+GGG | - | Chr5:20206180-20206199 | MsG0580025537.01.T01:CDS | 35.0% |
| !!! | AACTCTAGGTGGAGTTTTTT+AGG | - | Chr5:20206268-20206287 | MsG0580025537.01.T01:CDS | 35.0% |
| !!! | CTGCTTTATTTTCCAACTCT+AGG | - | Chr5:20206254-20206273 | MsG0580025537.01.T01:CDS | 35.0% |
| !!! | CTTTATTTTCCAACTCTAGG+TGG | - | Chr5:20206257-20206276 | MsG0580025537.01.T01:CDS | 35.0% |
| AAGAGAAGCCATGTTACTAG+TGG | - | Chr5:20206323-20206342 | MsG0580025537.01.T01:CDS | 40.0% | |
| CATTTATATCGACCCGGTAA+AGG | + | Chr5:20205941-20205960 | None:intergenic | 40.0% | |
| ! | CTGATGCTTTTCTTTCTGAC+AGG | - | Chr5:20206179-20206198 | MsG0580025537.01.T01:CDS | 40.0% |
| ! | CTGATGTAGTTGAAGCGTTT+CGG | - | Chr5:20206080-20206099 | MsG0580025537.01.T01:CDS | 40.0% |
| ! | TGATAGCGTGGACTTTGAAA+CGG | - | Chr5:20206141-20206160 | MsG0580025537.01.T01:CDS | 40.0% |
| !! | TGATATGCAGTTTGATAGCG+TGG | - | Chr5:20206129-20206148 | MsG0580025537.01.T01:CDS | 40.0% |
| !! | TTCCTCAGGTACCTTTGTTT+TGG | - | Chr5:20206024-20206043 | MsG0580025537.01.T01:CDS | 40.0% |
| ATTTGTGTCCGTGATTCCTC+AGG | - | Chr5:20206010-20206029 | MsG0580025537.01.T01:CDS | 45.0% | |
| TTATATCGACCCGGTAAAGG+AGG | + | Chr5:20205938-20205957 | None:intergenic | 45.0% | |
| !! | AGCCAAAACAAAGGTACCTG+AGG | + | Chr5:20206029-20206048 | None:intergenic | 45.0% |
| !! | CAAAGGTACCTGAGGAATCA+CGG | + | Chr5:20206021-20206040 | None:intergenic | 45.0% |
| AGGGACGACACATCCGAATT+TGG | - | Chr5:20206199-20206218 | MsG0580025537.01.T01:CDS | 50.0% | |
| CACAAATGCCAATACCCGTG+CGG | + | Chr5:20205997-20206016 | None:intergenic | 50.0% | |
| GGTTGCACGCTGCATTACAA+TGG | - | Chr5:20206101-20206120 | MsG0580025537.01.T01:CDS | 50.0% | |
| ! | TTTTAGGCGACAAGCCAACG+AGG | - | Chr5:20206284-20206303 | MsG0580025537.01.T01:CDS | 50.0% |
| GTTGAGGGGAGAAGAGAACG+CGG | + | Chr5:20205975-20205994 | None:intergenic | 55.0% | |
| ! | AAGAGCATGAGCAGCCTCGT+TGG | + | Chr5:20206301-20206320 | None:intergenic | 55.0% |
| CCAATACCCGTGCGGTTGAG+GGG | + | Chr5:20205989-20206008 | None:intergenic | 60.0% | |
| CTCTTCTCCCCTCAACCGCA+CGG | - | Chr5:20205979-20205998 | MsG0580025537.01.T01:CDS | 60.0% | |
| GCCAATACCCGTGCGGTTGA+GGG | + | Chr5:20205990-20206009 | None:intergenic | 60.0% | |
| TCTTCTCCCCTCAACCGCAC+GGG | - | Chr5:20205980-20205999 | MsG0580025537.01.T01:CDS | 60.0% | |
| TGCCAATACCCGTGCGGTTG+AGG | + | Chr5:20205991-20206010 | None:intergenic | 60.0% | |
| CCCCTCAACCGCACGGGTAT+TGG | - | Chr5:20205986-20206005 | MsG0580025537.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 20205929 | 20206342 | 20205929 | ID=MsG0580025537.01;Name=MsG0580025537.01 |
| Chr5 | mRNA | 20205929 | 20206342 | 20205929 | ID=MsG0580025537.01.T01;Parent=MsG0580025537.01;Name=MsG0580025537.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|0|1|0|137 |
| Chr5 | exon | 20205929 | 20206342 | 20205929 | ID=MsG0580025537.01.T01:exon:6706;Parent=MsG0580025537.01.T01 |
| Chr5 | CDS | 20205929 | 20206342 | 20205929 | ID=MsG0580025537.01.T01:cds;Parent=MsG0580025537.01.T01 |
| Gene Sequence |
| Protein sequence |
