Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025559.01.T01 | XP_003612918.1 | 98.316 | 297 | 5 | 0 | 9 | 305 | 1 | 297 | 0 | 591 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025559.01.T01 | MTR_5g030530 | 98.316 | 297 | 5 | 0 | 9 | 305 | 1 | 297 | 0.0 | 591 |
| MsG0580025559.01.T01 | MTR_8g066975 | 84.512 | 297 | 46 | 0 | 9 | 305 | 1 | 297 | 0.0 | 514 |
| MsG0580025559.01.T01 | MTR_4g088545 | 35.256 | 312 | 176 | 9 | 9 | 305 | 1 | 301 | 1.25e-44 | 154 |
| MsG0580025559.01.T01 | MTR_2g102010 | 34.437 | 302 | 179 | 6 | 7 | 304 | 8 | 294 | 1.96e-43 | 150 |
| MsG0580025559.01.T01 | MTR_4g012850 | 32.180 | 289 | 178 | 6 | 14 | 299 | 14 | 287 | 7.56e-40 | 141 |
| MsG0580025559.01.T01 | MTR_4g012850 | 28.643 | 199 | 124 | 6 | 14 | 207 | 107 | 292 | 3.67e-11 | 63.2 |
| MsG0580025559.01.T01 | MTR_4g088545 | 35.135 | 259 | 145 | 7 | 9 | 253 | 1 | 250 | 5.21e-35 | 128 |
| MsG0580025559.01.T01 | MTR_4g088545 | 27.979 | 193 | 127 | 4 | 114 | 301 | 10 | 195 | 1.85e-15 | 75.1 |
| MsG0580025559.01.T01 | MTR_8g027975 | 29.607 | 331 | 195 | 8 | 1 | 305 | 36 | 354 | 2.20e-30 | 117 |
| MsG0580025559.01.T01 | MTR_4g088545 | 36.765 | 204 | 112 | 6 | 9 | 204 | 1 | 195 | 1.40e-29 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025559.01.T01 | AT5G46800 | 76.000 | 300 | 69 | 1 | 9 | 305 | 1 | 300 | 9.20e-171 | 475 |
| MsG0580025559.01.T01 | AT5G46800 | 76.000 | 300 | 69 | 1 | 9 | 305 | 1 | 300 | 9.20e-171 | 475 |
| MsG0580025559.01.T01 | AT5G46800 | 76.000 | 300 | 69 | 1 | 9 | 305 | 1 | 300 | 9.20e-171 | 475 |
| MsG0580025559.01.T01 | AT2G33820 | 35.762 | 302 | 174 | 7 | 14 | 305 | 16 | 307 | 4.09e-50 | 168 |
| MsG0580025559.01.T01 | AT2G33820 | 25.366 | 205 | 139 | 6 | 103 | 301 | 5 | 201 | 6.30e-12 | 65.5 |
| MsG0580025559.01.T01 | AT2G33820 | 34.839 | 310 | 174 | 8 | 14 | 305 | 16 | 315 | 3.55e-47 | 161 |
| MsG0580025559.01.T01 | AT1G79900 | 32.014 | 278 | 162 | 6 | 30 | 304 | 30 | 283 | 1.41e-37 | 135 |
| MsG0580025559.01.T01 | AT2G33820 | 34.000 | 250 | 144 | 7 | 14 | 251 | 16 | 256 | 4.39e-35 | 128 |
| MsG0580025559.01.T01 | AT2G33820 | 25.000 | 208 | 142 | 6 | 103 | 304 | 5 | 204 | 8.30e-12 | 64.7 |
| MsG0580025559.01.T01 | AT2G33820 | 35.526 | 228 | 129 | 6 | 88 | 305 | 7 | 226 | 3.24e-33 | 122 |
Find 89 sgRNAs with CRISPR-Local
Find 148 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCTACTGAAGGGTTTAA+AGG | 0.332217 | 5:-20503980 | MsG0580025559.01.T01:CDS |
| AAAGGTCTTTATAAAGGTTT+TGG | 0.282854 | 5:-20503962 | MsG0580025559.01.T01:CDS |
| AAATCAGAAGGTGGCGTAAA+AGG | 0.329776 | 5:-20504271 | MsG0580025559.01.T01:CDS |
| AAGAAACATGCTGCATTGGC+AGG | 0.482397 | 5:+20503917 | None:intergenic |
| AAGAAGACAATAGCAGCTGA+AGG | 0.629491 | 5:-20505411 | MsG0580025559.01.T01:CDS |
| AAGGCAGCTACTGTAGCAAG+TGG | 0.605947 | 5:+20505357 | None:intergenic |
| ACAAAGCAATTTATTGCAGG+AGG | 0.612226 | 5:-20504172 | MsG0580025559.01.T01:CDS |
| ACAATAGCAGCTGAAGGTCC+AGG | 0.528414 | 5:-20505405 | MsG0580025559.01.T01:CDS |
| ACACTCTTAATAACATCAGT+TGG | 0.538878 | 5:+20504067 | None:intergenic |
| ACATGTCTGGCTACATCCAT+CGG | 0.595686 | 5:+20504298 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATCTCTGATTCATCTCTTT+AGG | - | Chr5:20504874-20504893 | MsG0580025559.01.T01:intron | 30.0% | |
| ACACTCTTAATAACATCAGT+TGG | + | Chr5:20505413-20505432 | None:intergenic | 30.0% | |
| ATCTCTGATTCATCTCTTTA+GGG | - | Chr5:20504875-20504894 | MsG0580025559.01.T01:intron | 30.0% | |
| ATTAAAAACACCATCATCTG+TGG | + | Chr5:20504310-20504329 | None:intergenic | 30.0% | |
| CTAGAAAGTTATTACATGAC+AGG | + | Chr5:20504405-20504424 | None:intergenic | 30.0% | |
| CTGTTCTAACAATATCTCTA+TGG | + | Chr5:20504544-20504563 | None:intergenic | 30.0% | |
| GGGTTTAAAGGTCTTTATAA+AGG | - | Chr5:20505509-20505528 | MsG0580025559.01.T01:CDS | 30.0% | |
| TCAATTGAACCAGAAAACTT+TGG | + | Chr5:20505461-20505480 | None:intergenic | 30.0% | |
| TGCATTTGATAAGTTCAGTA+GGG | + | Chr5:20504278-20504297 | None:intergenic | 30.0% | |
| TTCATATACACCAAACATTG+CGG | + | Chr5:20505282-20505301 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 20503883 | 20505616 | 20503883 | ID=MsG0580025559.01;Name=MsG0580025559.01 |
| Chr5 | mRNA | 20503883 | 20505616 | 20503883 | ID=MsG0580025559.01.T01;Parent=MsG0580025559.01;Name=MsG0580025559.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|305 |
| Chr5 | exon | 20505201 | 20505616 | 20505201 | ID=MsG0580025559.01.T01:exon:8949;Parent=MsG0580025559.01.T01 |
| Chr5 | exon | 20503883 | 20504384 | 20503883 | ID=MsG0580025559.01.T01:exon:8948;Parent=MsG0580025559.01.T01 |
| Chr5 | CDS | 20505201 | 20505616 | 20505201 | ID=MsG0580025559.01.T01:cds;Parent=MsG0580025559.01.T01 |
| Chr5 | CDS | 20503883 | 20504384 | 20503883 | ID=MsG0580025559.01.T01:cds;Parent=MsG0580025559.01.T01 |
| Gene Sequence |
>MsG0580025559.01.T01 CTCAAACAAAAACTGTTTTTTCAGATGGGAGATGTGGCCAAGGACCTTACTGCTGGAACTATTGGAGGGGCTGCACAACTGATAGTTGGTCATCCATTTGACACCATAAAGGTCAAGCTCCAAAGCCAGCCTACACCACTTCCTGGTCAGGTTCCAAAATATTCCGGTGCAATCGATGCTGTCAAGAAGACAATAGCAGCTGAAGGTCCAGGCGGATTATACAAAGGTATGGGAGCCCCACTTGCTACAGTAGCTGCCTTCAACGCTGTCCTGTTTACAGTTAGAGGGCAAATGGAAGCATTGTTGAGGTCACGTCCTGGCGATGTTCTCACATTAAATCAGCAGGTTGTTTGTGGAGCTGCAGCTGGACTTTCTGTTTCCTTTCTAGCTTGCCCTACTGAACTTATCAAATGCAGGTTACAAGCACAGAGTGCACTAGCTGGTTCAGGAACAGCGACATTGGCAGTGAAATATGGTGGACCGATGGATGTAGCCAGACATGTTCTCAAATCAGAAGGTGGCGTAAAAGGTCTTTTCAAGGGGTTAGTTCCCACTATGGCACGTGAAATACCTGGAAATGCCGCAATGTTTGGTGTATATGAAGCAACAAAGCAATTTATTGCAGGAGGCACCGATACCTCTGAACTCGGTAGAGGTTCTCTGATGCTTTCCGGAGGCTTGGCTGGAGCTGCTTTTTGGTTTATGGTGTATCCAACTGATGTTATTAAGAGTGTGATTCAAGTTGATGATTACAAAAATCCAAAGTTTTCTGGTTCAATTGATGCTTTCAGGAGGATAAAAGCTACTGAAGGGTTTAAAGGTCTTTATAAAGGTTTTGGTCCTGCAATGTGCAGAAGTGTTCCTGCCAATGCAGCATGTTTCTTGGCATATGAGATGACAAGATCAGCTTTAGGATGA |
| Protein sequence |
>MsG0580025559.01.T01 LKQKLFFQMGDVAKDLTAGTIGGAAQLIVGHPFDTIKVKLQSQPTPLPGQVPKYSGAIDAVKKTIAAEGPGGLYKGMGAPLATVAAFNAVLFTVRGQMEALLRSRPGDVLTLNQQVVCGAAAGLSVSFLACPTELIKCRLQAQSALAGSGTATLAVKYGGPMDVARHVLKSEGGVKGLFKGLVPTMAREIPGNAAMFGVYEATKQFIAGGTDTSELGRGSLMLSGGLAGAAFWFMVYPTDVIKSVIQVDDYKNPKFSGSIDAFRRIKATEGFKGLYKGFGPAMCRSVPANAACFLAYEMTRSALG* |
