AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025647.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025647.01.T01 MTR_5g032060 92.870 1122 80 0 18 1139 32 1153 0.0 2083
MsG0580025647.01.T01 MTR_8g471130 58.362 586 219 11 18 585 32 610 0.0 614
MsG0580025647.01.T01 MTR_8g471130 57.713 376 131 5 755 1128 635 984 1.16e-128 418
MsG0580025647.01.T01 MTR_5g066340 47.796 431 211 3 18 437 32 459 1.94e-110 369
MsG0580025647.01.T01 MTR_5g066340 38.914 442 199 13 748 1127 537 969 6.10e-79 280
MsG0580025647.01.T01 MTR_3g100190 42.829 502 259 9 17 500 28 519 6.31e-107 358
MsG0580025647.01.T01 MTR_3g100190 35.774 478 242 16 698 1128 490 949 4.33e-67 245
MsG0580025647.01.T01 MTR_1g071540 57.895 95 34 1 18 106 37 131 2.51e-27 121
MsG0580025647.01.T01 MTR_1g103160 55.769 104 38 3 18 121 35 130 7.48e-27 119
MsG0580025647.01.T01 MTR_1g103160 55.769 104 38 3 18 121 32 127 7.73e-27 119
MsG0580025647.01.T01 MTR_1g103160 55.340 103 38 3 19 121 1 95 3.30e-26 117
MsG0580025647.01.T01 MTR_5g006520 41.176 153 84 3 18 168 35 183 7.67e-25 113
MsG0580025647.01.T01 MTR_7g095410 55.208 96 36 1 18 106 35 130 1.12e-24 112
MsG0580025647.01.T01 MTR_7g095410 55.208 96 36 1 18 106 35 130 1.13e-24 112
MsG0580025647.01.T01 MTR_5g006520 41.176 153 84 3 18 168 35 183 1.43e-24 112
MsG0580025647.01.T01 MTR_7g095410 54.737 95 36 1 19 106 1 95 5.43e-24 110
MsG0580025647.01.T01 MTR_5g075490 56.579 76 29 2 18 89 43 118 3.95e-19 93.2
MsG0580025647.01.T01 MTR_3g467610 46.465 99 42 3 16 109 12 104 2.92e-18 90.1
MsG0580025647.01.T01 MTR_7g108070 49.351 77 34 1 17 93 33 104 6.11e-18 86.3
MsG0580025647.01.T01 MTR_1g088965 42.697 89 45 2 17 101 37 123 1.22e-14 78.6
MsG0580025647.01.T01 MTR_6g016950 55.738 61 27 0 896 956 2 62 1.24e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025647.01.T01 AT2G22560 52.814 462 207 7 18 472 32 489 1.01e-130 422
MsG0580025647.01.T01 AT2G22560 53.039 362 143 6 773 1132 611 947 1.76e-109 365
MsG0580025647.01.T01 AT5G10500 46.759 432 202 6 18 443 32 441 1.88e-102 343
MsG0580025647.01.T01 AT5G10500 43.353 346 151 9 783 1126 539 841 5.98e-66 240
MsG0580025647.01.T01 AT1G58215 44.068 472 223 9 18 470 32 481 2.46e-101 343
MsG0580025647.01.T01 AT1G58215 39.120 409 186 11 770 1122 527 928 5.32e-72 259
MsG0580025647.01.T01 AT1G09720 44.270 445 207 7 18 449 32 448 2.54e-93 320
MsG0580025647.01.T01 AT1G09720 37.772 413 207 12 752 1122 500 904 7.69e-70 253
MsG0580025647.01.T01 AT3G22790 48.466 163 67 5 17 175 33 182 3.85e-32 137
MsG0580025647.01.T01 AT3G22790 48.466 163 67 5 17 175 33 182 3.85e-32 137
MsG0580025647.01.T01 AT3G22790 48.466 163 67 5 17 175 33 182 3.85e-32 137
MsG0580025647.01.T01 AT1G03080 34.701 268 129 9 18 251 34 289 5.29e-27 120
MsG0580025647.01.T01 AT1G03080 34.701 268 129 9 18 251 34 289 5.29e-27 120
MsG0580025647.01.T01 AT1G03080 34.701 268 129 9 18 251 34 289 5.29e-27 120
MsG0580025647.01.T01 AT4G14760 41.420 169 80 2 17 185 33 182 3.96e-26 117
MsG0580025647.01.T01 AT4G14760 41.420 169 80 2 17 185 33 182 3.96e-26 117
MsG0580025647.01.T01 AT4G14760 41.420 169 80 2 17 185 33 182 3.96e-26 117
MsG0580025647.01.T01 AT4G14760 41.420 169 80 2 17 185 33 182 3.96e-26 117
MsG0580025647.01.T01 AT4G02710 54.455 101 39 2 17 110 34 134 1.08e-25 115
MsG0580025647.01.T01 AT2G30500 62.500 64 24 0 19 82 44 107 2.16e-18 90.5
MsG0580025647.01.T01 AT2G30500 62.500 64 24 0 19 82 44 107 2.16e-18 90.5
MsG0580025647.01.T01 AT2G30500 62.500 64 24 0 19 82 43 106 2.32e-18 90.5
MsG0580025647.01.T01 AT5G58320 42.453 106 49 2 19 113 1 105 4.39e-16 82.8
MsG0580025647.01.T01 AT5G58320 42.056 107 50 2 18 113 43 148 6.26e-16 82.8
MsG0580025647.01.T01 AT5G58320 42.056 107 50 2 18 113 43 148 6.26e-16 82.8
MsG0580025647.01.T01 AT5G58320 42.056 107 50 2 18 113 43 148 6.74e-16 82.8
MsG0580025647.01.T01 AT5G58320 42.056 107 50 2 18 113 43 148 6.74e-16 82.8
MsG0580025647.01.T01 AT1G03470 61.111 54 21 0 17 70 28 81 1.39e-15 79.0
MsG0580025647.01.T01 AT1G03470 61.111 54 21 0 17 70 28 81 1.39e-15 79.0
MsG0580025647.01.T01 AT1G03470 61.111 54 21 0 17 70 28 81 1.39e-15 79.0
MsG0580025647.01.T01 AT1G03470 61.111 54 21 0 17 70 28 81 1.39e-15 79.0
MsG0580025647.01.T01 AT2G47920 53.571 56 26 0 17 72 30 85 3.05e-14 73.9
MsG0580025647.01.T01 AT2G47920 53.571 56 26 0 17 72 30 85 3.05e-14 73.9

Find 202 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCATTGCTTCTGCCTTTC+CGG 0.093031 5:-21750641 MsG0580025647.01.T01:CDS
TACCTTATCTTCTACATTAT+TGG 0.096083 5:+21748965 None:intergenic
AACCCTTCTGGTATTCTTCT+TGG 0.168843 5:+21750568 None:intergenic
TAGAGGAGAACTTGACTTTC+TGG 0.174932 5:-21748140 MsG0580025647.01.T01:CDS
TCGTCTTCTTCATCCATAAA+TGG 0.198222 5:+21750610 None:intergenic
GAGAATATGGATTGTTTCTT+TGG 0.218760 5:+21747553 None:intergenic
TTTCTGCTCAATCCAGATTT+AGG 0.220618 5:+21750433 None:intergenic
TGATTGGCGGAAAATGTTTC+TGG 0.235953 5:-21748529 MsG0580025647.01.T01:CDS
TCCGGATCGAGTCCCATTTA+TGG 0.245615 5:-21750623 MsG0580025647.01.T01:CDS
ATAAAGTTGAATGAGCAATT+TGG 0.246073 5:-21747670 MsG0580025647.01.T01:CDS
CGTCTTCTAGAGTTTGAATA+TGG 0.272380 5:+21749759 None:intergenic
ATTCATGAGAGAGCCTAATT+TGG 0.278007 5:+21750087 None:intergenic
TCTTAACATCTTTGTAATTC+CGG 0.280501 5:+21748454 None:intergenic
AAAGATGTTAAGGCCAAATT+AGG 0.292243 5:-21750100 MsG0580025647.01.T01:CDS
TGTCTTAGATGGACTTTGTT+TGG 0.294491 5:+21748608 None:intergenic
CGCCAATCAGGTTCATCATC+TGG 0.311425 5:+21748543 None:intergenic
GGTCGCTCCTTCCATTTCTA+AGG 0.312821 5:+21748827 None:intergenic
CCTCTTAGGACGTTTATCTT+TGG 0.313473 5:-21747583 MsG0580025647.01.T01:CDS
TAAAGTTGAATGAGCAATTT+GGG 0.321315 5:-21747669 MsG0580025647.01.T01:CDS
TGATCTGCCTTGTTGCTTGT+TGG 0.323645 5:+21748672 None:intergenic
GATCTGCCTTGTTGCTTGTT+GGG 0.323801 5:+21748673 None:intergenic
TCTTATCTCTTGATCTTTCA+AGG 0.326289 5:+21748341 None:intergenic
CTCTAGAATTTCATCCATCT+TGG 0.332054 5:+21748158 None:intergenic
AGATTATTCTCTGTCTTAGA+TGG 0.335664 5:+21748597 None:intergenic
AGGGGTCATGTAGGAGAATA+TGG 0.335695 5:+21747540 None:intergenic
TATTCTGATCTTCAACAGTT+TGG 0.337118 5:+21749648 None:intergenic
AATGAAGAGGTTAAGGTTGC+AGG 0.339353 5:-21749356 MsG0580025647.01.T01:CDS
TACAATGGAACTTTCCCTTC+AGG 0.347554 5:+21749089 None:intergenic
GGTATTCTTCTTGGAGTTCT+CGG 0.349199 5:+21750577 None:intergenic
ATGCATTTGTTATCTCCTCT+TGG 0.352051 5:+21747860 None:intergenic
ACAGGACTTGCAGTTGTTAC+AGG 0.353641 5:-21749951 MsG0580025647.01.T01:CDS
TTAGAAGATGCGGTAAATTC+AGG 0.354346 5:-21749005 MsG0580025647.01.T01:CDS
CTCGAGCCAGGGTTCCTCTT+AGG 0.355622 5:-21747597 MsG0580025647.01.T01:CDS
TGTCGCAGCAAGAGAGGAAT+TGG 0.362341 5:+21749898 None:intergenic
TTCAAAGGAACTTCTCCTTC+AGG 0.364298 5:+21749281 None:intergenic
CAAAACCTCACCTTGGAACT+TGG 0.365277 5:+21747786 None:intergenic
GCAATTGTTCTAATGTCTCT+TGG 0.366720 5:+21750176 None:intergenic
ACGTCAGAAAAGGAACAAAT+TGG 0.368077 5:-21749032 MsG0580025647.01.T01:CDS
CTCGAGTTTCTGACTCTCTT+AGG 0.374054 5:+21747614 None:intergenic
TATATGCAAAACTGCAGATA+TGG 0.379015 5:-21750848 MsG0580025647.01.T01:CDS
AGAACAATTGCAAGAGAAAC+AGG 0.379513 5:-21750164 MsG0580025647.01.T01:CDS
TTTGGTGTTGAATTTCTTTG+TGG 0.379900 5:+21750489 None:intergenic
ACTTCTGAAGCTCCTAAATC+TGG 0.387556 5:-21750445 MsG0580025647.01.T01:CDS
AAATCTTTAAGTGGAGGCTT+TGG 0.391437 5:+21750532 None:intergenic
CACAAGCTCATCAATCTTCT+CGG 0.392471 5:+21749868 None:intergenic
GTAACTGATTCATCACAAAA+TGG 0.394730 5:-21748921 MsG0580025647.01.T01:CDS
GCAACCTTAACCTCTTCATT+TGG 0.394954 5:+21749359 None:intergenic
TCGACAAAGTTTATTAGCTC+TGG 0.397416 5:+21750739 None:intergenic
CTCTCTTGCTGCGACAGAAA+TGG 0.402021 5:-21749891 MsG0580025647.01.T01:CDS
GCAACCTTAACCTCCTCATT+TGG 0.402170 5:+21749167 None:intergenic
GCAGCCAAATGAAGAGGTTA+AGG 0.405597 5:-21749363 MsG0580025647.01.T01:CDS
ATCGACTCTCATCTTGTTCT+AGG 0.406625 5:+21749987 None:intergenic
AAGACATTGTCTTGATTCAA+TGG 0.406648 5:+21749458 None:intergenic
GTGTTTCGATGAAGTGAGTT+TGG 0.409146 5:+21749618 None:intergenic
ATAATTCCATTGCTAGGTAC+TGG 0.409185 5:-21750327 MsG0580025647.01.T01:CDS
GAACGATCAACTGAGAGAAA+TGG 0.412838 5:-21749702 MsG0580025647.01.T01:CDS
GTGCTGAAGATGACGACTTC+AGG 0.414898 5:-21747828 MsG0580025647.01.T01:CDS
CCAAGCCTTATCCCACAAAA+TGG 0.415663 5:-21748885 MsG0580025647.01.T01:CDS
AAGTCAGAAAAGGAACAAAA+TGG 0.417843 5:-21749227 MsG0580025647.01.T01:CDS
TCCATAAATGGGACTCGATC+CGG 0.419186 5:+21750622 None:intergenic
GGTCTCTAGTACCTTAGAAA+TGG 0.419764 5:-21748838 MsG0580025647.01.T01:CDS
AACTGCAGATATGGAGGAAA+AGG 0.419818 5:-21750839 MsG0580025647.01.T01:CDS
TCTAAGACAGAGAATAATCT+TGG 0.422444 5:-21748594 MsG0580025647.01.T01:CDS
CAAAAGGATAAAAGATGTTA+AGG 0.424297 5:-21750110 MsG0580025647.01.T01:CDS
AATTACAACTACCGAAAATC+AGG 0.424331 5:-21748709 MsG0580025647.01.T01:CDS
GCAGCCAAATGAGGAGGTTA+AGG 0.428551 5:-21749171 MsG0580025647.01.T01:CDS
GCAGCCAAGTGAGGAGGTTA+AGG 0.430687 5:-21749555 MsG0580025647.01.T01:CDS
CAAGTCCTCATTTCCTTCCA+TGG 0.439327 5:+21748284 None:intergenic
ACACGCAGAAAATAATCTTA+AGG 0.443704 5:-21748859 MsG0580025647.01.T01:CDS
TCTTCAACTTGAAATTGAAA+AGG 0.447622 5:-21747698 MsG0580025647.01.T01:CDS
CAGCTATTGAAGAGAAGTTC+CGG 0.448222 5:-21748182 MsG0580025647.01.T01:CDS
CCTCACTTGGCTGCACACTT+TGG 0.448827 5:+21749564 None:intergenic
ACAGAAACTGATGAACTTCA+AGG 0.449917 5:-21749782 MsG0580025647.01.T01:CDS
GAGATACAAAAGAATTCATC+AGG 0.453271 5:-21749326 MsG0580025647.01.T01:CDS
GAGAGTCAGAAACTCGAGCC+AGG 0.461335 5:-21747609 MsG0580025647.01.T01:CDS
TTCCAATAATGTAGAAGATA+AGG 0.463291 5:-21748967 MsG0580025647.01.T01:CDS
CAGATACAAAAGAATTCATC+AGG 0.465550 5:-21749518 MsG0580025647.01.T01:CDS
TGATATGTGATCATATCGTT+CGG 0.467800 5:+21750687 None:intergenic
AGTTCAACTCTGCCTCTGCC+AGG 0.469072 5:-21747504 MsG0580025647.01.T01:CDS
CGTCTTCTTCATCCATAAAT+GGG 0.469571 5:+21750611 None:intergenic
TGAATCAGTTACCTTGAAAT+CGG 0.470577 5:+21748932 None:intergenic
AAACCAAAATTGAGTCCAAA+AGG 0.471635 5:-21750126 MsG0580025647.01.T01:CDS
GAGATTTGAAACCACTATCA+AGG 0.472040 5:-21748085 MsG0580025647.01.T01:CDS
TCTGTCAAGTGTTTGTATAT+CGG 0.472598 5:+21747970 None:intergenic
TCCGTTCTGCAAGATGAACT+TGG 0.473025 5:-21750265 MsG0580025647.01.T01:CDS
AACTGAAATAACAGTCTGGT+TGG 0.480143 5:-21747941 MsG0580025647.01.T01:CDS
GAAGAGGCTCGCCGAAATAG+AGG 0.483954 5:-21748433 MsG0580025647.01.T01:CDS
CATGGAAGGAAATGAGGACT+TGG 0.484904 5:-21748283 MsG0580025647.01.T01:CDS
GGCAGAAGCAATGGTGGTGT+TGG 0.486014 5:+21750648 None:intergenic
TCGATTGACTCAACAGATAC+AGG 0.491764 5:-21749969 MsG0580025647.01.T01:CDS
AAGTCAGAAAAGGAACATAC+TGG 0.492755 5:-21749419 MsG0580025647.01.T01:CDS
AAGGATCCAAGTGTTTGTCT+TGG 0.493378 5:+21748403 None:intergenic
GGTTTGCAAATCCTTGATAG+TGG 0.494682 5:+21748074 None:intergenic
CAAGGCAGATCATAGTTCTT+CGG 0.495119 5:-21748661 MsG0580025647.01.T01:CDS
ACTTCAAAGCGACAAGAAAG+TGG 0.495489 5:-21747640 MsG0580025647.01.T01:CDS
GTATCAAAACTCGAGGAAAA+CGG 0.497584 5:-21748048 MsG0580025647.01.T01:CDS
TTGAACTTCCTATGCATTCG+AGG 0.498639 5:+21747520 None:intergenic
TTCAACTGCAATTGCAAACA+AGG 0.504680 5:+21748384 None:intergenic
AACATCAGATACCGATTTCA+AGG 0.507198 5:-21748943 MsG0580025647.01.T01:CDS
TCAATAGCTGATGGAGATGC+AGG 0.508933 5:+21748195 None:intergenic
TTGCAGTTCTTCTTTCATCA+AGG 0.509652 5:+21747909 None:intergenic
TTCAAACTGAAATAACAGTC+TGG 0.510044 5:-21747945 MsG0580025647.01.T01:CDS
ATATTTCGATTCAGCCTGGC+CGG 0.510502 5:+21749115 None:intergenic
CAGATACAAAAGAATTCATC+CGG 0.513987 5:-21749134 MsG0580025647.01.T01:CDS
TGATGAAAAGAAGTCAGAAA+AGG 0.515171 5:-21749429 MsG0580025647.01.T01:CDS
TGTGAACAAAGTGATTAGCT+TGG 0.517595 5:-21749849 MsG0580025647.01.T01:CDS
CTTAACCTTGGTAATAGTGA+TGG 0.519243 5:-21748756 MsG0580025647.01.T01:CDS
TGATGTAAAGACGTCAGAAA+AGG 0.519966 5:-21749042 MsG0580025647.01.T01:CDS
ATAAAACTCATAGAGGAAGA+TGG 0.521667 5:-21750805 MsG0580025647.01.T01:CDS
GAAAATGTTTCTGGATGGAA+TGG 0.524836 5:-21748520 MsG0580025647.01.T01:CDS
ACAAAAGAATTCATCCGGCC+AGG 0.526294 5:-21749129 MsG0580025647.01.T01:CDS
AAGACACTGTCTTGATTCAA+AGG 0.527445 5:+21749266 None:intergenic
TGATGTAAGGAAGTCAGAAA+AGG 0.530663 5:-21749237 MsG0580025647.01.T01:CDS
AACTTCTCTTCAATAGCTGA+TGG 0.531264 5:+21748186 None:intergenic
AACCAGATGATGAACCTGAT+TGG 0.531970 5:-21748545 MsG0580025647.01.T01:CDS
CCAAAGATAAACGTCCTAAG+AGG 0.532228 5:+21747583 None:intergenic
AATTGGTTTGTTAGAAGATG+CGG 0.536438 5:-21749015 MsG0580025647.01.T01:CDS
GTGTCTTACTGAATGATGTA+AGG 0.536986 5:-21749250 MsG0580025647.01.T01:CDS
GGTTAAGGTTGCAGGTTCAT+CGG 0.537156 5:-21749348 MsG0580025647.01.T01:CDS
GAGTGTAAAAGTGCGCTTTG+AGG 0.538882 5:-21749927 MsG0580025647.01.T01:CDS
ACAAAAGAATTCATCAGGCC+AGG 0.539159 5:-21749321 MsG0580025647.01.T01:CDS
ACAAAAGAATTCATCAGGCC+AGG 0.539159 5:-21749513 MsG0580025647.01.T01:CDS
GGAAACTGAAGATAAGATCA+AGG 0.541694 5:-21750305 MsG0580025647.01.T01:CDS
GAATTTCATCCATCTTGGAC+CGG 0.542356 5:+21748163 None:intergenic
GTTCATATTTCGATTCAGCC+TGG 0.546546 5:+21749111 None:intergenic
AAGGAAGCACTTGAAGAGGT+TGG 0.552162 5:-21750412 MsG0580025647.01.T01:CDS
CTTTGTCGAAGAAACATACA+AGG 0.553261 5:-21750725 MsG0580025647.01.T01:CDS
TCATGTTCAAAACCTCACCT+TGG 0.553386 5:+21747779 None:intergenic
GAGCAGCTTCTGTCATCAAA+CGG 0.554248 5:+21750209 None:intergenic
CATATCGTTCGGCTAAAGCT+CGG 0.554769 5:+21750698 None:intergenic
GAAGTCGTCATCTTCAGCAC+TGG 0.559834 5:+21747831 None:intergenic
TTCAGTTTGTCATCAAACAA+AGG 0.560181 5:+21750874 None:intergenic
GAATCGAAATATGAACCTGA+AGG 0.563796 5:-21749104 MsG0580025647.01.T01:CDS
TAAAGAGCAGCCAAATGAAG+AGG 0.564170 5:-21749369 MsG0580025647.01.T01:CDS
CGTATGATAATTCCATTGCT+AGG 0.564881 5:-21750333 MsG0580025647.01.T01:CDS
TGAATGTCATGTTCTTCTTG+CGG 0.565347 5:+21748240 None:intergenic
AATGTTTCTGGATGGAATGG+AGG 0.565880 5:-21748517 MsG0580025647.01.T01:CDS
AGATGAAAAGCAGCCAAATG+AGG 0.566843 5:-21749180 MsG0580025647.01.T01:CDS
TAATTCCATTGCTAGGTACT+GGG 0.567450 5:-21750326 MsG0580025647.01.T01:CDS
ATCCGGAAAGGCAGAAGCAA+TGG 0.567731 5:+21750639 None:intergenic
TCCAAGTTCATCTTGCAGAA+CGG 0.569248 5:+21750264 None:intergenic
GAATCAAAAGACGAAGCTGA+AGG 0.569525 5:-21749488 MsG0580025647.01.T01:CDS
TAAATCTGGATTGAGCAGAA+AGG 0.569928 5:-21750431 MsG0580025647.01.T01:CDS
TGGAGTTGATCACAAAGAGC+AGG 0.570341 5:-21748574 MsG0580025647.01.T01:CDS
AGTTTCCCAGTACCTAGCAA+TGG 0.571846 5:+21750321 None:intergenic
TGAACTTCCTATGCATTCGA+GGG 0.572906 5:+21747521 None:intergenic
ACATTGTCTTCTTGATACAA+TGG 0.573074 5:+21749074 None:intergenic
AGAGAAGTTCCGGTCCAAGA+TGG 0.574046 5:-21748172 MsG0580025647.01.T01:CDS
TTTCGATGAAGTGAGTTTGG+AGG 0.575342 5:+21749621 None:intergenic
CCAAAGTGTGCAGCCAAGTG+AGG 0.576455 5:-21749564 MsG0580025647.01.T01:CDS
CTGAGTACACTAATACTCTC+CGG 0.577725 5:-21748473 MsG0580025647.01.T01:CDS
GAGTGCTATAAAACTCATAG+AGG 0.580176 5:-21750812 MsG0580025647.01.T01:CDS
TATCAAGCAGCCAAGTTCCA+AGG 0.580861 5:-21747796 MsG0580025647.01.T01:CDS
GGAACCTTAACCTCCTCACT+TGG 0.583660 5:+21749551 None:intergenic
GATAACCATGGAAGGAAATG+AGG 0.584672 5:-21748289 MsG0580025647.01.T01:CDS
TTTATCTCTACAAACGGTGA+AGG 0.584872 5:-21750371 MsG0580025647.01.T01:CDS
ACGACTCTGATCCCTGGCAG+AGG 0.585708 5:+21747492 None:intergenic
CAGATGATGAACCTGATTGG+CGG 0.587019 5:-21748542 MsG0580025647.01.T01:CDS
GTTCAACTCTGCCTCTGCCA+GGG 0.591458 5:-21747503 MsG0580025647.01.T01:CDS
GAATCAAAACATGCACCTGA+AGG 0.591948 5:-21749296 MsG0580025647.01.T01:CDS
ATGCAAAACTGCAGATATGG+AGG 0.592734 5:-21750845 MsG0580025647.01.T01:CDS
AGAGTCAGAAACTCGAGCCA+GGG 0.593387 5:-21747608 MsG0580025647.01.T01:CDS
CAGAAAGGAAGCACTTGAAG+AGG 0.593487 5:-21750416 MsG0580025647.01.T01:CDS
CGGAAAGGCAGAAGCAATGG+TGG 0.594326 5:+21750642 None:intergenic
AGCAGCCAAGTTCCAAGGTG+AGG 0.594801 5:-21747791 MsG0580025647.01.T01:CDS
TCTAGTACCTTAGAAATGGA+AGG 0.594942 5:-21748834 MsG0580025647.01.T01:CDS
GTTGCTGATGAACTTCAAGC+AGG 0.596873 5:-21747739 MsG0580025647.01.T01:CDS
ACATGACCCCTCGAATGCAT+AGG 0.599671 5:-21747528 MsG0580025647.01.T01:CDS
CTGCAAGATGAACTTGGAGA+AGG 0.601778 5:-21750259 MsG0580025647.01.T01:CDS
CAAGAGAAGACTCTTAACCT+TGG 0.602953 5:-21748768 MsG0580025647.01.T01:CDS
CATTTCTGTCGCAGCAAGAG+AGG 0.605285 5:+21749892 None:intergenic
AGAGCAAATCCAAAGAGCCA+AGG 0.606637 5:-21750048 MsG0580025647.01.T01:CDS
TGGCGGAAAATGTTTCTGGA+TGG 0.607525 5:-21748525 MsG0580025647.01.T01:CDS
AAATGGGACTCGATCCGGAA+AGG 0.610310 5:+21750627 None:intergenic
GCAAGAGAAACAGGAAAGGT+CGG 0.611162 5:-21750155 MsG0580025647.01.T01:CDS
CTACAAACGACTCTGATCCC+TGG 0.612125 5:+21747486 None:intergenic
TTGCAATATTAATCGCCTCT+CGG 0.612145 5:-21749594 MsG0580025647.01.T01:CDS
AATCGAAATATGAACCTGAA+GGG 0.613676 5:-21749103 MsG0580025647.01.T01:CDS
GAAAACGGAAAGTCTTCTGA+AGG 0.614118 5:-21748033 MsG0580025647.01.T01:CDS
AATCAAAAGACGAAGCTGAA+GGG 0.615697 5:-21749487 MsG0580025647.01.T01:CDS
ATTACAAAGATGTTAAGAAG+AGG 0.617444 5:-21748449 MsG0580025647.01.T01:CDS
ATCTTTGTGTGTCATCCAAG+AGG 0.618053 5:-21747875 MsG0580025647.01.T01:CDS
TTCAGAAGTAGCCGCCGCTG+TGG 0.620833 5:+21750459 None:intergenic
CAAGATGGATGAAATTCTAG+AGG 0.623067 5:-21748157 MsG0580025647.01.T01:CDS
GAGCAAATCCAAAGAGCCAA+GGG 0.624606 5:-21750047 MsG0580025647.01.T01:CDS
ATTCTGTAAAATCAGATGCA+CGG 0.632633 5:-21747993 MsG0580025647.01.T01:CDS
ACTCCAAGAAGAATACCAGA+AGG 0.633933 5:-21750571 MsG0580025647.01.T01:CDS
ATGCATTCGAGGGGTCATGT+AGG 0.638953 5:+21747531 None:intergenic
CTCCAAGAAGAATACCAGAA+GGG 0.641166 5:-21750570 MsG0580025647.01.T01:CDS
CAATATTAATCGCCTCTCGG+TGG 0.644608 5:-21749591 MsG0580025647.01.T01:CDS
AAGTGTGCAGCCAAGTGAGG+AGG 0.648838 5:-21749561 MsG0580025647.01.T01:CDS
AACCGAGGTATCAAAACTCG+AGG 0.649634 5:-21748055 MsG0580025647.01.T01:CDS
CACTTTGGACTTCCACCGAG+AGG 0.651124 5:+21749579 None:intergenic
AAACGTCCTAAGAGGAACCC+TGG 0.651807 5:+21747591 None:intergenic
GAACTTCCTATGCATTCGAG+GGG 0.659380 5:+21747522 None:intergenic
TGAAAAGCAGCCAAATGAGG+AGG 0.663959 5:-21749177 MsG0580025647.01.T01:CDS
ATGGATGAAGAAGACGACGA+TGG 0.668655 5:-21750604 MsG0580025647.01.T01:CDS
TCAGCACCCAACAAGCAACA+AGG 0.671103 5:-21748679 MsG0580025647.01.T01:CDS
AGATGCGGTAAATTCAGGTG+AGG 0.684741 5:-21749000 MsG0580025647.01.T01:CDS
AATTGCAAGAGAAACAGGAA+AGG 0.688544 5:-21750159 MsG0580025647.01.T01:CDS
AATGGAAGGAGCGACCGCAG+TGG 0.698144 5:-21748820 MsG0580025647.01.T01:CDS
TATCAAGGATTTGCAAACCG+AGG 0.711750 5:-21748070 MsG0580025647.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACACTTTTTAAATCTTTAAG+TGG + Chr5:21747849-21747868 None:intergenic 20.0%
! ATAAAGTTGAATGAGCAATT+TGG - Chr5:21750699-21750718 MsG0580025647.01.T01:CDS 25.0%
! ATTACAAAGATGTTAAGAAG+AGG - Chr5:21749920-21749939 MsG0580025647.01.T01:CDS 25.0%
! CAAAAGGATAAAAGATGTTA+AGG - Chr5:21748259-21748278 MsG0580025647.01.T01:CDS 25.0%
! TAAAGTTGAATGAGCAATTT+GGG - Chr5:21750700-21750719 MsG0580025647.01.T01:CDS 25.0%
! TACCTTATCTTCTACATTAT+TGG + Chr5:21749407-21749426 None:intergenic 25.0%
! TCTTAACATCTTTGTAATTC+CGG + Chr5:21749918-21749937 None:intergenic 25.0%
! TCTTCAACTTGAAATTGAAA+AGG - Chr5:21750671-21750690 MsG0580025647.01.T01:CDS 25.0%
! TTCCAATAATGTAGAAGATA+AGG - Chr5:21749402-21749421 MsG0580025647.01.T01:CDS 25.0%
!! ACAGATTTTATCTCTACAAA+CGG - Chr5:21747992-21748011 MsG0580025647.01.T01:CDS 25.0%
!! CTTAACATCTTTTATCCTTT+TGG + Chr5:21748261-21748280 None:intergenic 25.0%
!! CTTTTTAAATCTTTAAGTGG+AGG + Chr5:21747846-21747865 None:intergenic 25.0%
AAACCAAAATTGAGTCCAAA+AGG - Chr5:21748243-21748262 MsG0580025647.01.T01:CDS 30.0%
AAAGATGTTAAGGCCAAATT+AGG - Chr5:21748269-21748288 MsG0580025647.01.T01:CDS 30.0%
AAGACATTGTCTTGATTCAA+TGG + Chr5:21748914-21748933 None:intergenic 30.0%
AAGTCAGAAAAGGAACAAAA+TGG - Chr5:21749142-21749161 MsG0580025647.01.T01:CDS 30.0%
AATCGAAATATGAACCTGAA+GGG - Chr5:21749266-21749285 MsG0580025647.01.T01:CDS 30.0%
AATTACAACTACCGAAAATC+AGG - Chr5:21749660-21749679 MsG0580025647.01.T01:CDS 30.0%
ACACGCAGAAAATAATCTTA+AGG - Chr5:21749510-21749529 MsG0580025647.01.T01:CDS 30.0%
ACATTGTCTTCTTGATACAA+TGG + Chr5:21749298-21749317 None:intergenic 30.0%
AGATTATTCTCTGTCTTAGA+TGG + Chr5:21749775-21749794 None:intergenic 30.0%
ATAAAACTCATAGAGGAAGA+TGG - Chr5:21747564-21747583 MsG0580025647.01.T01:CDS 30.0%
ATTCTGTAAAATCAGATGCA+CGG - Chr5:21750376-21750395 MsG0580025647.01.T01:CDS 30.0%
CAGATACAAAAGAATTCATC+AGG - Chr5:21748851-21748870 MsG0580025647.01.T01:CDS 30.0%
CAGATACAAAAGAATTCATC+CGG - Chr5:21748851-21748870 MsG0580025647.01.T01:CDS 30.0%
GAGATACAAAAGAATTCATC+AGG - Chr5:21749043-21749062 MsG0580025647.01.T01:CDS 30.0%
TATATGCAAAACTGCAGATA+TGG - Chr5:21747521-21747540 MsG0580025647.01.T01:CDS 30.0%
TATTCTGATCTTCAACAGTT+TGG + Chr5:21748724-21748743 None:intergenic 30.0%
TCTGTCAAGTGTTTGTATAT+CGG + Chr5:21750402-21750421 None:intergenic 30.0%
TCTTATCTCTTGATCTTTCA+AGG + Chr5:21750031-21750050 None:intergenic 30.0%
TGAATCAGTTACCTTGAAAT+CGG + Chr5:21749440-21749459 None:intergenic 30.0%
TGATATGTGATCATATCGTT+CGG + Chr5:21747685-21747704 None:intergenic 30.0%
TGATGAAAAGAAGTCAGAAA+AGG - Chr5:21748940-21748959 MsG0580025647.01.T01:CDS 30.0%
TTCAAACTGAAATAACAGTC+TGG - Chr5:21750424-21750443 MsG0580025647.01.T01:CDS 30.0%
TTCAGTTTGTCATCAAACAA+AGG + Chr5:21747498-21747517 None:intergenic 30.0%
! AAAAAGTGTAGAAGAGAGAA+AGG - Chr5:21748358-21748377 MsG0580025647.01.T01:CDS 30.0%
! GAGAATATGGATTGTTTCTT+TGG + Chr5:21750819-21750838 None:intergenic 30.0%
! GTAACTGATTCATCACAAAA+TGG - Chr5:21749448-21749467 MsG0580025647.01.T01:CDS 30.0%
! TATCCTTTTGGACTCAATTT+TGG + Chr5:21748249-21748268 None:intergenic 30.0%
! TCTAAGACAGAGAATAATCT+TGG - Chr5:21749775-21749794 MsG0580025647.01.T01:CDS 30.0%
! TGATCTGTTAACTCTTTTAG+AGG + Chr5:21749583-21749602 None:intergenic 30.0%
! TTTACATCTCTTTTTACCCT+TGG + Chr5:21748341-21748360 None:intergenic 30.0%
! TTTTGTATCTGTGATGAATC+TGG + Chr5:21748842-21748861 None:intergenic 30.0%
!! AATTGGTTTGTTAGAAGATG+CGG - Chr5:21749354-21749373 MsG0580025647.01.T01:CDS 30.0%
!! TTGGTTTTTGTCCTCTATTT+CGG + Chr5:21749950-21749969 None:intergenic 30.0%
!! TTTGGTGTTGAATTTCTTTG+TGG + Chr5:21747883-21747902 None:intergenic 30.0%
AAATCTTTAAGTGGAGGCTT+TGG + Chr5:21747840-21747859 None:intergenic 35.0%
AACTGAAATAACAGTCTGGT+TGG - Chr5:21750428-21750447 MsG0580025647.01.T01:CDS 35.0%
AACTTCTCTTCAATAGCTGA+TGG + Chr5:21750186-21750205 None:intergenic 35.0%
AAGACACTGTCTTGATTCAA+AGG + Chr5:21749106-21749125 None:intergenic 35.0%
AAGTCAGAAAAGGAACATAC+TGG - Chr5:21748950-21748969 MsG0580025647.01.T01:CDS 35.0%
AATCAAAAGACGAAGCTGAA+GGG - Chr5:21748882-21748901 MsG0580025647.01.T01:CDS 35.0%
AATTGCAAGAGAAACAGGAA+AGG - Chr5:21748210-21748229 MsG0580025647.01.T01:CDS 35.0%
ACAGAAACTGATGAACTTCA+AGG - Chr5:21748587-21748606 MsG0580025647.01.T01:CDS 35.0%
ACGTCAGAAAAGGAACAAAT+TGG - Chr5:21749337-21749356 MsG0580025647.01.T01:CDS 35.0%
AGAACAATTGCAAGAGAAAC+AGG - Chr5:21748205-21748224 MsG0580025647.01.T01:CDS 35.0%
ATAATTCCATTGCTAGGTAC+TGG - Chr5:21748042-21748061 MsG0580025647.01.T01:CDS 35.0%
ATTCATGAGAGAGCCTAATT+TGG + Chr5:21748285-21748304 None:intergenic 35.0%
CAAAAACCAAGACAAACACT+TGG - Chr5:21749960-21749979 MsG0580025647.01.T01:CDS 35.0%
CAAGATGGATGAAATTCTAG+AGG - Chr5:21750212-21750231 MsG0580025647.01.T01:CDS 35.0%
CGTATGATAATTCCATTGCT+AGG - Chr5:21748036-21748055 MsG0580025647.01.T01:CDS 35.0%
CGTCTTCTTCATCCATAAAT+GGG + Chr5:21747761-21747780 None:intergenic 35.0%
CTTTGTCGAAGAAACATACA+AGG - Chr5:21747644-21747663 MsG0580025647.01.T01:CDS 35.0%
GAATCGAAATATGAACCTGA+AGG - Chr5:21749265-21749284 MsG0580025647.01.T01:CDS 35.0%
GAGTGCTATAAAACTCATAG+AGG - Chr5:21747557-21747576 MsG0580025647.01.T01:CDS 35.0%
GCAATTGTTCTAATGTCTCT+TGG + Chr5:21748196-21748215 None:intergenic 35.0%
GGAAACTGAAGATAAGATCA+AGG - Chr5:21748064-21748083 MsG0580025647.01.T01:CDS 35.0%
GTATCAAAACTCGAGGAAAA+CGG - Chr5:21750321-21750340 MsG0580025647.01.T01:CDS 35.0%
GTGTCTTACTGAATGATGTA+AGG - Chr5:21749119-21749138 MsG0580025647.01.T01:CDS 35.0%
TAAATCTGGATTGAGCAGAA+AGG - Chr5:21747938-21747957 MsG0580025647.01.T01:CDS 35.0%
TAATTCCATTGCTAGGTACT+GGG - Chr5:21748043-21748062 MsG0580025647.01.T01:CDS 35.0%
TCGACAAAGTTTATTAGCTC+TGG + Chr5:21747633-21747652 None:intergenic 35.0%
TCGTCTTCTTCATCCATAAA+TGG + Chr5:21747762-21747781 None:intergenic 35.0%
TCTAGTACCTTAGAAATGGA+AGG - Chr5:21749535-21749554 MsG0580025647.01.T01:CDS 35.0%
TGAATGTCATGTTCTTCTTG+CGG + Chr5:21750132-21750151 None:intergenic 35.0%
TGATGTAAAGACGTCAGAAA+AGG - Chr5:21749327-21749346 MsG0580025647.01.T01:CDS 35.0%
TGATGTAAGGAAGTCAGAAA+AGG - Chr5:21749132-21749151 MsG0580025647.01.T01:CDS 35.0%
TTAGAAGATGCGGTAAATTC+AGG - Chr5:21749364-21749383 MsG0580025647.01.T01:CDS 35.0%
TTCAACTGCAATTGCAAACA+AGG + Chr5:21749988-21750007 None:intergenic 35.0%
TTGCAATATTAATCGCCTCT+CGG - Chr5:21748775-21748794 MsG0580025647.01.T01:CDS 35.0%
TTGCAGTTCTTCTTTCATCA+AGG + Chr5:21750463-21750482 None:intergenic 35.0%
TTTATCTCTACAAACGGTGA+AGG - Chr5:21747998-21748017 MsG0580025647.01.T01:CDS 35.0%
TTTCTGCTCAATCCAGATTT+AGG + Chr5:21747939-21747958 None:intergenic 35.0%
! CTTAACCTTGGTAATAGTGA+TGG - Chr5:21749613-21749632 MsG0580025647.01.T01:CDS 35.0%
! CTTTTCCATCACTATTACCA+AGG + Chr5:21749621-21749640 None:intergenic 35.0%
! GAGATTTGAAACCACTATCA+AGG - Chr5:21750284-21750303 MsG0580025647.01.T01:CDS 35.0%
! TAGTCTTTTGCAGATAACCA+TGG - Chr5:21750068-21750087 MsG0580025647.01.T01:CDS 35.0%
! TGTCTTAGATGGACTTTGTT+TGG + Chr5:21749764-21749783 None:intergenic 35.0%
! TGTGAACAAAGTGATTAGCT+TGG - Chr5:21748520-21748539 MsG0580025647.01.T01:CDS 35.0%
!! AACATCAGATACCGATTTCA+AGG - Chr5:21749426-21749445 MsG0580025647.01.T01:CDS 35.0%
!! ATGCATTTGTTATCTCCTCT+TGG + Chr5:21750512-21750531 None:intergenic 35.0%
!! CGTCTTCTAGAGTTTGAATA+TGG + Chr5:21748613-21748632 None:intergenic 35.0%
!! CTCTAGAATTTCATCCATCT+TGG + Chr5:21750214-21750233 None:intergenic 35.0%
!! GAAAATGTTTCTGGATGGAA+TGG - Chr5:21749849-21749868 MsG0580025647.01.T01:CDS 35.0%
!!! TGAGGTTTTGAACATGAAAC+AGG - Chr5:21750596-21750615 MsG0580025647.01.T01:CDS 35.0%
AACCAGATGATGAACCTGAT+TGG - Chr5:21749824-21749843 MsG0580025647.01.T01:CDS 40.0%
AACCCTTCTGGTATTCTTCT+TGG + Chr5:21747804-21747823 None:intergenic 40.0%
AACTGCAGATATGGAGGAAA+AGG - Chr5:21747530-21747549 MsG0580025647.01.T01:CDS 40.0%
AAGGATCCAAGTGTTTGTCT+TGG + Chr5:21749969-21749988 None:intergenic 40.0%
AATGAAGAGGTTAAGGTTGC+AGG - Chr5:21749013-21749032 MsG0580025647.01.T01:CDS 40.0%
ACAAAAGAATTCATCAGGCC+AGG - Chr5:21748856-21748875 MsG0580025647.01.T01:CDS 40.0%
ACTCCAAGAAGAATACCAGA+AGG - Chr5:21747798-21747817 MsG0580025647.01.T01:CDS 40.0%
ACTTCAAAGCGACAAGAAAG+TGG - Chr5:21750729-21750748 MsG0580025647.01.T01:CDS 40.0%
AGATGAAAAGCAGCCAAATG+AGG - Chr5:21749189-21749208 MsG0580025647.01.T01:CDS 40.0%
ATCTTTGTGTGTCATCCAAG+AGG - Chr5:21750494-21750513 MsG0580025647.01.T01:CDS 40.0%
ATGCAAAACTGCAGATATGG+AGG - Chr5:21747524-21747543 MsG0580025647.01.T01:CDS 40.0%
CAAGAGAAGACTCTTAACCT+TGG - Chr5:21749601-21749620 MsG0580025647.01.T01:CDS 40.0%
CAAGGCAGATCATAGTTCTT+CGG - Chr5:21749708-21749727 MsG0580025647.01.T01:CDS 40.0%
CACAAGCTCATCAATCTTCT+CGG + Chr5:21748504-21748523 None:intergenic 40.0%
CAGCTATTGAAGAGAAGTTC+CGG - Chr5:21750187-21750206 MsG0580025647.01.T01:CDS 40.0%
CCAAAGATAAACGTCCTAAG+AGG + Chr5:21750789-21750808 None:intergenic 40.0%
CCTCTTAGGACGTTTATCTT+TGG - Chr5:21750786-21750805 MsG0580025647.01.T01:CDS 40.0%
CTCCAAGAAGAATACCAGAA+GGG - Chr5:21747799-21747818 MsG0580025647.01.T01:CDS 40.0%
CTGAGTACACTAATACTCTC+CGG - Chr5:21749896-21749915 MsG0580025647.01.T01:CDS 40.0%
GAAAACGGAAAGTCTTCTGA+AGG - Chr5:21750336-21750355 MsG0580025647.01.T01:CDS 40.0%
GAACGATCAACTGAGAGAAA+TGG - Chr5:21748667-21748686 MsG0580025647.01.T01:CDS 40.0%
GAATCAAAACATGCACCTGA+AGG - Chr5:21749073-21749092 MsG0580025647.01.T01:CDS 40.0%
GAATCAAAAGACGAAGCTGA+AGG - Chr5:21748881-21748900 MsG0580025647.01.T01:CDS 40.0%
GAATTTCATCCATCTTGGAC+CGG + Chr5:21750209-21750228 None:intergenic 40.0%
GATAACCATGGAAGGAAATG+AGG - Chr5:21750080-21750099 MsG0580025647.01.T01:CDS 40.0%
GCAACCTTAACCTCTTCATT+TGG + Chr5:21749013-21749032 None:intergenic 40.0%
GGTATTCTTCTTGGAGTTCT+CGG + Chr5:21747795-21747814 None:intergenic 40.0%
GGTCTCTAGTACCTTAGAAA+TGG - Chr5:21749531-21749550 MsG0580025647.01.T01:CDS 40.0%
GGTTTGCAAATCCTTGATAG+TGG + Chr5:21750298-21750317 None:intergenic 40.0%
GTGTTTCGATGAAGTGAGTT+TGG + Chr5:21748754-21748773 None:intergenic 40.0%
GTTCATATTTCGATTCAGCC+TGG + Chr5:21749261-21749280 None:intergenic 40.0%
TAAAGAGCAGCCAAATGAAG+AGG - Chr5:21749000-21749019 MsG0580025647.01.T01:CDS 40.0%
TACAATGGAACTTTCCCTTC+AGG + Chr5:21749283-21749302 None:intergenic 40.0%
TAGAGGAGAACTTGACTTTC+TGG - Chr5:21750229-21750248 MsG0580025647.01.T01:CDS 40.0%
TATCAAGGATTTGCAAACCG+AGG - Chr5:21750299-21750318 MsG0580025647.01.T01:CDS 40.0%
TCATGTTCAAAACCTCACCT+TGG + Chr5:21750593-21750612 None:intergenic 40.0%
TCCAAGTTCATCTTGCAGAA+CGG + Chr5:21748108-21748127 None:intergenic 40.0%
TGAACTTCCTATGCATTCGA+GGG + Chr5:21750851-21750870 None:intergenic 40.0%
TGATTGGCGGAAAATGTTTC+TGG - Chr5:21749840-21749859 MsG0580025647.01.T01:CDS 40.0%
TTCAAAGGAACTTCTCCTTC+AGG + Chr5:21749091-21749110 None:intergenic 40.0%
TTGAACTTCCTATGCATTCG+AGG + Chr5:21750852-21750871 None:intergenic 40.0%
TTTCGATGAAGTGAGTTTGG+AGG + Chr5:21748751-21748770 None:intergenic 40.0%
! AGAAACATTTTCCGCCAATC+AGG + Chr5:21749841-21749860 None:intergenic 40.0%
! ATCGACTCTCATCTTGTTCT+AGG + Chr5:21748385-21748404 None:intergenic 40.0%
! CTTTTGCAGATAACCATGGA+AGG - Chr5:21750072-21750091 MsG0580025647.01.T01:CDS 40.0%
! GTAGACCATTTTGTGGGATA+AGG + Chr5:21749492-21749511 None:intergenic 40.0%
! TCGATTGACTCAACAGATAC+AGG - Chr5:21748400-21748419 MsG0580025647.01.T01:CDS 40.0%
!! AATGTTTCTGGATGGAATGG+AGG - Chr5:21749852-21749871 MsG0580025647.01.T01:CDS 40.0%
!! ACTTCTGAAGCTCCTAAATC+TGG - Chr5:21747924-21747943 MsG0580025647.01.T01:CDS 40.0%
!! CTGATCGACTTCCTGATTTT+CGG + Chr5:21749674-21749693 None:intergenic 40.0%
!! TAGAGGTTTGTTTTCCACTG+CGG + Chr5:21749566-21749585 None:intergenic 40.0%
!! TCTTTTTACCCTTGGCTCTT+TGG + Chr5:21748333-21748352 None:intergenic 40.0%
!! TTCCTCGAGTTTTGATACCT+CGG + Chr5:21750319-21750338 None:intergenic 40.0%
!!! GTGTTTGTTTTGAACCCTTC+TGG + Chr5:21747816-21747835 None:intergenic 40.0%
ACAAAAGAATTCATCCGGCC+AGG - Chr5:21749240-21749259 MsG0580025647.01.T01:CDS 45.0%
ACAGGACTTGCAGTTGTTAC+AGG - Chr5:21748418-21748437 MsG0580025647.01.T01:CDS 45.0%
AGAGCAAATCCAAAGAGCCA+AGG - Chr5:21748321-21748340 MsG0580025647.01.T01:CDS 45.0%
AGATGCGGTAAATTCAGGTG+AGG - Chr5:21749369-21749388 MsG0580025647.01.T01:CDS 45.0%
AGGGGTCATGTAGGAGAATA+TGG + Chr5:21750832-21750851 None:intergenic 45.0%
AGTTTCCCAGTACCTAGCAA+TGG + Chr5:21748051-21748070 None:intergenic 45.0%
ATATTTCGATTCAGCCTGGC+CGG + Chr5:21749257-21749276 None:intergenic 45.0%
ATGGATGAAGAAGACGACGA+TGG - Chr5:21747765-21747784 MsG0580025647.01.T01:CDS 45.0%
CAAAACCTCACCTTGGAACT+TGG + Chr5:21750586-21750605 None:intergenic 45.0%
CAAGTCCTCATTTCCTTCCA+TGG + Chr5:21750088-21750107 None:intergenic 45.0%
CAATATTAATCGCCTCTCGG+TGG - Chr5:21748778-21748797 MsG0580025647.01.T01:CDS 45.0%
CAGATGATGAACCTGATTGG+CGG - Chr5:21749827-21749846 MsG0580025647.01.T01:CDS 45.0%
CATATCGTTCGGCTAAAGCT+CGG + Chr5:21747674-21747693 None:intergenic 45.0%
CATGGAAGGAAATGAGGACT+TGG - Chr5:21750086-21750105 MsG0580025647.01.T01:CDS 45.0%
CTCGAGTTTCTGACTCTCTT+AGG + Chr5:21750758-21750777 None:intergenic 45.0%
CTGCAAGATGAACTTGGAGA+AGG - Chr5:21748110-21748129 MsG0580025647.01.T01:CDS 45.0%
GAACTTCCTATGCATTCGAG+GGG + Chr5:21750850-21750869 None:intergenic 45.0%
GAGCAAATCCAAAGAGCCAA+GGG - Chr5:21748322-21748341 MsG0580025647.01.T01:CDS 45.0%
GAGCAGCTTCTGTCATCAAA+CGG + Chr5:21748163-21748182 None:intergenic 45.0%
GCAACCTTAACCTCCTCATT+TGG + Chr5:21749205-21749224 None:intergenic 45.0%
GCAAGAGAAACAGGAAAGGT+CGG - Chr5:21748214-21748233 MsG0580025647.01.T01:CDS 45.0%
GCAGCCAAATGAAGAGGTTA+AGG - Chr5:21749006-21749025 MsG0580025647.01.T01:CDS 45.0%
GGTTAAGGTTGCAGGTTCAT+CGG - Chr5:21749021-21749040 MsG0580025647.01.T01:CDS 45.0%
GTTGCTGATGAACTTCAAGC+AGG - Chr5:21750630-21750649 MsG0580025647.01.T01:CDS 45.0%
TATCAAGCAGCCAAGTTCCA+AGG - Chr5:21750573-21750592 MsG0580025647.01.T01:CDS 45.0%
TCAATAGCTGATGGAGATGC+AGG + Chr5:21750177-21750196 None:intergenic 45.0%
TCCATAAATGGGACTCGATC+CGG + Chr5:21747750-21747769 None:intergenic 45.0%
TCCGTTCTGCAAGATGAACT+TGG - Chr5:21748104-21748123 MsG0580025647.01.T01:CDS 45.0%
TGAAAAGCAGCCAAATGAGG+AGG - Chr5:21749192-21749211 MsG0580025647.01.T01:CDS 45.0%
! AACCGAGGTATCAAAACTCG+AGG - Chr5:21750314-21750333 MsG0580025647.01.T01:CDS 45.0%
! CCAAGCCTTATCCCACAAAA+TGG - Chr5:21749484-21749503 MsG0580025647.01.T01:CDS 45.0%
! CCATTTTGTGGGATAAGGCT+TGG + Chr5:21749487-21749506 None:intergenic 45.0%
! CTGCGTGTAGACCATTTTGT+GGG + Chr5:21749498-21749517 None:intergenic 45.0%
! GAGTGTAAAAGTGCGCTTTG+AGG - Chr5:21748442-21748461 MsG0580025647.01.T01:CDS 45.0%
! GATCTGCCTTGTTGCTTGTT+GGG + Chr5:21749699-21749718 None:intergenic 45.0%
! TCTGCGTGTAGACCATTTTG+TGG + Chr5:21749499-21749518 None:intergenic 45.0%
! TGATCTGCCTTGTTGCTTGT+TGG + Chr5:21749700-21749719 None:intergenic 45.0%
! TGGAGTTGATCACAAAGAGC+AGG - Chr5:21749795-21749814 MsG0580025647.01.T01:CDS 45.0%
!! AAGGAAGCACTTGAAGAGGT+TGG - Chr5:21747957-21747976 MsG0580025647.01.T01:CDS 45.0%
!! CAGAAAGGAAGCACTTGAAG+AGG - Chr5:21747953-21747972 MsG0580025647.01.T01:CDS 45.0%
!! CTTCGTCTTTTGATTCAGCC+TGG + Chr5:21748877-21748896 None:intergenic 45.0%
!! TGGCGGAAAATGTTTCTGGA+TGG - Chr5:21749844-21749863 MsG0580025647.01.T01:CDS 45.0%
!!! GTGCATGTTTTGATTCAGCC+TGG + Chr5:21749069-21749088 None:intergenic 45.0%
AAACGTCCTAAGAGGAACCC+TGG + Chr5:21750781-21750800 None:intergenic 50.0%
AAATGGGACTCGATCCGGAA+AGG + Chr5:21747745-21747764 None:intergenic 50.0%
ACATGACCCCTCGAATGCAT+AGG - Chr5:21750841-21750860 MsG0580025647.01.T01:CDS 50.0%
AGAGAAGTTCCGGTCCAAGA+TGG - Chr5:21750197-21750216 MsG0580025647.01.T01:CDS 50.0%
AGAGTCAGAAACTCGAGCCA+GGG - Chr5:21750761-21750780 MsG0580025647.01.T01:CDS 50.0%
ATCCGGAAAGGCAGAAGCAA+TGG + Chr5:21747733-21747752 None:intergenic 50.0%
ATGCATTCGAGGGGTCATGT+AGG + Chr5:21750841-21750860 None:intergenic 50.0%
CACCAAAAAGTTCGCCACAG+CGG - Chr5:21747896-21747915 MsG0580025647.01.T01:CDS 50.0%
CACCATTGCTTCTGCCTTTC+CGG - Chr5:21747728-21747747 MsG0580025647.01.T01:CDS 50.0%
CATTTCTGTCGCAGCAAGAG+AGG + Chr5:21748480-21748499 None:intergenic 50.0%
CGCCAATCAGGTTCATCATC+TGG + Chr5:21749829-21749848 None:intergenic 50.0%
CTACAAACGACTCTGATCCC+TGG + Chr5:21750886-21750905 None:intergenic 50.0%
CTCTCTTGCTGCGACAGAAA+TGG - Chr5:21748478-21748497 MsG0580025647.01.T01:CDS 50.0%
GAAGTCGTCATCTTCAGCAC+TGG + Chr5:21750541-21750560 None:intergenic 50.0%
GCAGCCAAATGAGGAGGTTA+AGG - Chr5:21749198-21749217 MsG0580025647.01.T01:CDS 50.0%
GGAACCTTAACCTCCTCACT+TGG + Chr5:21748821-21748840 None:intergenic 50.0%
GGTCGCTCCTTCCATTTCTA+AGG + Chr5:21749545-21749564 None:intergenic 50.0%
GTGCTGAAGATGACGACTTC+AGG - Chr5:21750541-21750560 MsG0580025647.01.T01:CDS 50.0%
TCAGCACCCAACAAGCAACA+AGG - Chr5:21749690-21749709 MsG0580025647.01.T01:CDS 50.0%
TCCGGATCGAGTCCCATTTA+TGG - Chr5:21747746-21747765 MsG0580025647.01.T01:CDS 50.0%
TGTCGCAGCAAGAGAGGAAT+TGG + Chr5:21748474-21748493 None:intergenic 50.0%
AAGTGTGCAGCCAAGTGAGG+AGG - Chr5:21748808-21748827 MsG0580025647.01.T01:CDS 55.0%
AGCAGCCAAGTTCCAAGGTG+AGG - Chr5:21750578-21750597 MsG0580025647.01.T01:CDS 55.0%
AGTTCAACTCTGCCTCTGCC+AGG - Chr5:21750865-21750884 MsG0580025647.01.T01:CDS 55.0%
CAAAAAGTTCGCCACAGCGG+CGG - Chr5:21747899-21747918 MsG0580025647.01.T01:CDS 55.0%
CACTTTGGACTTCCACCGAG+AGG + Chr5:21748793-21748812 None:intergenic 55.0%
CCAAAGTGTGCAGCCAAGTG+AGG - Chr5:21748805-21748824 MsG0580025647.01.T01:CDS 55.0%
CCTCACTTGGCTGCACACTT+TGG + Chr5:21748808-21748827 None:intergenic 55.0%
CGGAAAGGCAGAAGCAATGG+TGG + Chr5:21747730-21747749 None:intergenic 55.0%
GAAGAGGCTCGCCGAAATAG+AGG - Chr5:21749936-21749955 MsG0580025647.01.T01:CDS 55.0%
GAGAGTCAGAAACTCGAGCC+AGG - Chr5:21750760-21750779 MsG0580025647.01.T01:CDS 55.0%
GCAGCCAAGTGAGGAGGTTA+AGG - Chr5:21748814-21748833 MsG0580025647.01.T01:CDS 55.0%
GTTCAACTCTGCCTCTGCCA+GGG - Chr5:21750866-21750885 MsG0580025647.01.T01:CDS 55.0%
! GGCAGAAGCAATGGTGGTGT+TGG + Chr5:21747724-21747743 None:intergenic 55.0%
AATGGAAGGAGCGACCGCAG+TGG - Chr5:21749549-21749568 MsG0580025647.01.T01:CDS 60.0%
ACGACTCTGATCCCTGGCAG+AGG + Chr5:21750880-21750899 None:intergenic 60.0%
CTCGAGCCAGGGTTCCTCTT+AGG - Chr5:21750772-21750791 MsG0580025647.01.T01:CDS 60.0%
TTCAGAAGTAGCCGCCGCTG+TGG + Chr5:21747913-21747932 None:intergenic 60.0%
! CGCCGCTGTGGCGAACTTTT+TGG + Chr5:21747901-21747920 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 21747486 21750905 21747486 ID=MsG0580025647.01;Name=MsG0580025647.01
Chr5 mRNA 21747486 21750905 21747486 ID=MsG0580025647.01.T01;Parent=MsG0580025647.01;Name=MsG0580025647.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|1139
Chr5 exon 21747486 21750905 21747486 ID=MsG0580025647.01.T01:exon:9286;Parent=MsG0580025647.01.T01
Chr5 CDS 21747486 21750905 21747486 ID=MsG0580025647.01.T01:cds;Parent=MsG0580025647.01.T01
Gene Sequence

>MsG0580025647.01.T01

ATGTATAAGCCTTTGTTTGATGACAAACTGAAATGTATATGCAAAACTGCAGATATGGAGGAAAAGGTACAGAGTGCTATAAAACTCATAGAGGAAGATGGAGACTCATTTGCAAAGAGAGCAGAAATGTATTACAAAAAGAGACCAGAGCTAATAAACTTTGTCGAAGAAACATACAAGGCTTACCGAGCTTTAGCCGAACGATATGATCACATATCAAAAGAGTTGCAGAATGCCAACACCACCATTGCTTCTGCCTTTCCGGATCGAGTCCCATTTATGGATGAAGAAGACGACGATGGATCGCCGAGAACTCCAAGAAGAATACCAGAAGGGTTCAAAACAAACACTCCAAAGCCTCCACTTAAAGATTTAAAAAGTGTAGAAACAGCAGCCACAAAGAAATTCAACACCAAAAAGTTCGCCACAGCGGCGGCTACTTCTGAAGCTCCTAAATCTGGATTGAGCAGAAAGGAAGCACTTGAAGAGGTTGGCAAGCTACAGAAACAGATTTTATCTCTACAAACGGTGAAGGAGTTCGTGAAAAGCTCGTATGATAATTCCATTGCTAGGTACTGGGAAACTGAAGATAAGATCAAGGAATTACAAGAGAGAGTTTCCGTTCTGCAAGATGAACTTGGAGAAGGTATCGTTATCGAAGATGATGAAGCACGCCGTTTGATGACAGAAGCTGCTCTTAAATCATGCCAAGAGACATTAGAACAATTGCAAGAGAAACAGGAAAGGTCGGTTGATGAAACCAAAATTGAGTCCAAAAGGATAAAAGATGTTAAGGCCAAATTAGGCTCTCTCATGAATGAGTTTCAATATGATCAGAGCAAATCCAAAGAGCCAAGGGTAAAAAGAGATGTAAAAAGTGTAGAAGAGAGAAAGGACCTAGAACAAGATGAGAGTCGATTGACTCAACAGATACAGGACTTGCAGTTGTTACAGGAGAGTGTAAAAGTGCGCTTTGAGGCTAGCTCCAATTCCTCTCTTGCTGCGACAGAAATGGCCGAGAAGATTGATGAGCTTGTGAACAAAGTGATTAGCTTGGAAACTTCAGTTTCATCTCAGACTGCTCTAGTATATAGTTTGAGAACAGAAACTGATGAACTTCAAGGCCATATTCAAACTCTAGAAGACGATAAAGAAAGTCTGATAAAAGATAAAAATAAACTGAACGATCAACTGAGAGAAATGGAACAAAAGATGCTTGCAGTACAAGATTTAAACCAAACTGTTGAAGATCAGAATAGCAACCTCCAAACTCACTTCATCGAAACACATTGCAATATTAATCGCCTCTCGGTGGAAGTCCAAAGTGTGCAGCCAAGTGAGGAGGTTAAGGTTCCAGATTCATCACAGATACAAAAGAATTCATCAGGCCAGGCTGAATCAAAAGACGAAGCTGAAGGGAAAGTTCCATTGAATCAAGACAATGTCTTGATGAATGATGAAAAGAAGTCAGAAAAGGAACATACTGGTTTGTTAGAAGATGCATTAAATTCAGATAAAGAGCAGCCAAATGAAGAGGTTAAGGTTGCAGGTTCATCGGAGATACAAAAGAATTCATCAGGCCAGGCTGAATCAAAACATGCACCTGAAGGAGAAGTTCCTTTGAATCAAGACAGTGTCTTACTGAATGATGTAAGGAAGTCAGAAAAGGAACAAAATGGTTTGTTAGAAGATGCAGTAAATTCAGATGAAAAGCAGCCAAATGAGGAGGTTAAGGTTGCAGATTCATCGCAGATACAAAAGAATTCATCCGGCCAGGCTGAATCGAAATATGAACCTGAAGGGAAAGTTCCATTGTATCAAGAAGACAATGTCTTGCTGAATGATGTAAAGACGTCAGAAAAGGAACAAATTGGTTTGTTAGAAGATGCGGTAAATTCAGGTGAGGAGCTTAACATTTCCAATAATGTAGAAGATAAGGTAACATCAGATACCGATTTCAAGGTAACTGATTCATCACAAAATGGTGTACAGCAACAACCAAGCCTTATCCCACAAAATGGTCTACACGCAGAAAATAATCTTAAGGTCTCTAGTACCTTAGAAATGGAAGGAGCGACCGCAGTGGAAAACAAACCTCTAAAAGAGTTAACAGATCAAGAGAAGACTCTTAACCTTGGTAATAGTGATGGAAAAGAAACAGTTGCTATGAGTACAATTACAACTACCGAAAATCAGGAAGTCGATCAGCACCCAACAAGCAACAAGGCAGATCATAGTTCTTCGGAGAGTTTTGAGAAACTGCAAGAAAATGATGCCAAACAAAGTCCATCTAAGACAGAGAATAATCTTGGAGTTGATCACAAAGAGCAGGAAACAAAACCAGATGATGAACCTGATTGGCGGAAAATGTTTCTGGATGGAATGGAGGATAGAGAAAAAGCGCTTCTAACTGAGTACACTAATACTCTCCGGAATTACAAAGATGTTAAGAAGAGGCTCGCCGAAATAGAGGACAAAAACCAAGACAAACACTTGGATCCTTGTTTGCAATTGCAGTTGAATGAGCTGAAGACATCTAATTCCTTGAAAGATCAAGAGATAAGAATTTTACATCAGAAACTTAGTCTTTTGCAGATAACCATGGAAGGAAATGAGGACTTGGCTGACTCAACTTCAGTTCTACCGCAAGAAGAACATGACATTCAGAAACTACTAAAAATTGATCAACCTGCATCTCCATCAGCTATTGAAGAGAAGTTCCGGTCCAAGATGGATGAAATTCTAGAGGAGAACTTGACTTTCTGGTTAAAATTTAGTACAACTTATGCTGAGATACAGAGATTTGAAACCACTATCAAGGATTTGCAAACCGAGGTATCAAAACTCGAGGAAAACGGAAAGTCTTCTGAAGGCAGTGCAGTCATAAAGCATTCTGTAAAATCAGATGCACGGCCGATATACAAACACTTGACAGAGATTCAAACTGAAATAACAGTCTGGTTGGAAAAAAGCGCCTTGATGAAAGAAGAACTGCAACGTAGACTATCATCTTTGTGTGTCATCCAAGAGGAGATAACAAATGCATTGAAAGCCAGTGCTGAAGATGACGACTTCAGGTTCACGAGCTATCAAGCAGCCAAGTTCCAAGGTGAGGTTTTGAACATGAAACAGGAAAACAATAAAGTTGCTGATGAACTTCAAGCAGGTCTAGATATTGCAACATCTCTTCAACTTGAAATTGAAAAGGCACTTATAAAGTTGAATGAGCAATTTGGGTTTTCAACTTCAAAGCGACAAGAAAGTGGAGACCTAAGAGAGTCAGAAACTCGAGCCAGGGTTCCTCTTAGGACGTTTATCTTTGGTGTGAAACCAAAGAAACAATCCATATTCTCCTACATGACCCCTCGAATGCATAGGAAGTTCAACTCTGCCTCTGCCAGGGATCAGAGTCGTTTGTAG
Protein sequence

>MsG0580025647.01.T01

MYKPLFDDKLKCICKTADMEEKVQSAIKLIEEDGDSFAKRAEMYYKKRPELINFVEETYKAYRALAERYDHISKELQNANTTIASAFPDRVPFMDEEDDDGSPRTPRRIPEGFKTNTPKPPLKDLKSVETAATKKFNTKKFATAAATSEAPKSGLSRKEALEEVGKLQKQILSLQTVKEFVKSSYDNSIARYWETEDKIKELQERVSVLQDELGEGIVIEDDEARRLMTEAALKSCQETLEQLQEKQERSVDETKIESKRIKDVKAKLGSLMNEFQYDQSKSKEPRVKRDVKSVEERKDLEQDESRLTQQIQDLQLLQESVKVRFEASSNSSLAATEMAEKIDELVNKVISLETSVSSQTALVYSLRTETDELQGHIQTLEDDKESLIKDKNKLNDQLREMEQKMLAVQDLNQTVEDQNSNLQTHFIETHCNINRLSVEVQSVQPSEEVKVPDSSQIQKNSSGQAESKDEAEGKVPLNQDNVLMNDEKKSEKEHTGLLEDALNSDKEQPNEEVKVAGSSEIQKNSSGQAESKHAPEGEVPLNQDSVLLNDVRKSEKEQNGLLEDAVNSDEKQPNEEVKVADSSQIQKNSSGQAESKYEPEGKVPLYQEDNVLLNDVKTSEKEQIGLLEDAVNSGEELNISNNVEDKVTSDTDFKVTDSSQNGVQQQPSLIPQNGLHAENNLKVSSTLEMEGATAVENKPLKELTDQEKTLNLGNSDGKETVAMSTITTTENQEVDQHPTSNKADHSSSESFEKLQENDAKQSPSKTENNLGVDHKEQETKPDDEPDWRKMFLDGMEDREKALLTEYTNTLRNYKDVKKRLAEIEDKNQDKHLDPCLQLQLNELKTSNSLKDQEIRILHQKLSLLQITMEGNEDLADSTSVLPQEEHDIQKLLKIDQPASPSAIEEKFRSKMDEILEENLTFWLKFSTTYAEIQRFETTIKDLQTEVSKLEENGKSSEGSAVIKHSVKSDARPIYKHLTEIQTEITVWLEKSALMKEELQRRLSSLCVIQEEITNALKASAEDDDFRFTSYQAAKFQGEVLNMKQENNKVADELQAGLDIATSLQLEIEKALIKLNEQFGFSTSKRQESGDLRESETRARVPLRTFIFGVKPKKQSIFSYMTPRMHRKFNSASARDQSRL*