AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025831.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025831.01.T01 MTR_5g035070 97.523 444 11 0 1 444 1 444 0.0 904
MsG0580025831.01.T01 MTR_5g035090 67.808 438 137 4 3 440 1 434 0.0 624
MsG0580025831.01.T01 MTR_5g035150 64.828 435 151 2 5 437 1 435 0.0 593
MsG0580025831.01.T01 MTR_5g035080 61.893 412 137 6 38 444 37 433 0.0 524
MsG0580025831.01.T01 MTR_3g109840 57.273 440 163 9 4 440 5 422 3.25e-179 507
MsG0580025831.01.T01 MTR_8g104970 51.188 463 176 7 18 434 8 466 2.23e-168 482
MsG0580025831.01.T01 MTR_7g033230 56.952 374 151 5 68 438 248 614 2.53e-158 461
MsG0580025831.01.T01 MTR_7g033230 56.952 374 151 5 68 438 318 684 2.73e-157 461
MsG0580025831.01.T01 MTR_5g035140 61.255 271 98 4 174 441 7 273 1.87e-119 350
MsG0580025831.01.T01 MTR_6g016880 41.646 413 223 10 30 434 18 420 4.84e-112 337
MsG0580025831.01.T01 MTR_8g099300 39.401 434 243 9 5 433 1 419 9.04e-107 323
MsG0580025831.01.T01 MTR_7g019120 39.529 425 234 10 17 434 13 421 8.66e-106 321
MsG0580025831.01.T01 MTR_8g027900 44.915 354 185 5 84 433 208 555 4.89e-104 320
MsG0580025831.01.T01 MTR_6g007777 36.864 472 249 13 8 441 5 465 8.52e-97 299
MsG0580025831.01.T01 MTR_6g016875 38.889 414 216 10 30 434 24 409 3.41e-91 283
MsG0580025831.01.T01 MTR_2g016060 40.548 365 191 14 84 435 51 402 6.57e-86 268
MsG0580025831.01.T01 MTR_1g076690 34.903 361 212 9 87 435 223 572 2.93e-68 228
MsG0580025831.01.T01 MTR_6g061710 32.692 416 249 10 43 442 123 523 1.32e-67 224
MsG0580025831.01.T01 MTR_7g094750 33.973 365 204 15 87 435 91 434 3.29e-55 190
MsG0580025831.01.T01 MTR_0136s0010 35.246 366 197 14 87 435 170 512 3.08e-54 189
MsG0580025831.01.T01 MTR_7g093450 35.135 370 199 14 87 438 248 594 3.74e-54 190
MsG0580025831.01.T01 MTR_5g035120 52.874 174 71 3 5 177 2 165 6.85e-53 175
MsG0580025831.01.T01 MTR_2g022560 34.066 364 210 15 86 437 96 441 1.61e-48 172
MsG0580025831.01.T01 MTR_4g126950 30.326 399 243 13 57 442 79 455 4.33e-47 168
MsG0580025831.01.T01 MTR_4g121560 29.340 409 244 16 48 433 67 453 3.89e-46 166
MsG0580025831.01.T01 MTR_5g059400 31.070 383 210 15 86 442 174 528 1.48e-45 166
MsG0580025831.01.T01 MTR_1g116130 31.070 383 210 15 86 442 174 528 1.48e-45 166
MsG0580025831.01.T01 MTR_8g069790 30.495 364 217 11 87 436 178 519 6.58e-45 164
MsG0580025831.01.T01 MTR_5g033800 29.333 375 222 12 78 435 137 485 4.10e-44 161
MsG0580025831.01.T01 MTR_7g109550 32.402 358 198 14 87 430 137 464 6.54e-44 160
MsG0580025831.01.T01 MTR_8g093530 29.745 353 212 10 87 431 90 414 2.06e-43 158
MsG0580025831.01.T01 MTR_4g016940 29.834 362 229 11 82 433 81 427 2.30e-43 157
MsG0580025831.01.T01 MTR_5g071350 30.601 366 226 11 75 429 47 395 7.48e-43 155
MsG0580025831.01.T01 MTR_1g058380 28.404 426 248 17 29 435 10 397 8.21e-42 153
MsG0580025831.01.T01 MTR_7g068920 31.768 362 222 13 82 433 75 421 2.10e-41 152
MsG0580025831.01.T01 MTR_7g070060 29.235 366 226 14 80 429 57 405 7.81e-41 150
MsG0580025831.01.T01 MTR_7g094750 35.600 250 136 8 87 323 91 328 1.52e-40 147
MsG0580025831.01.T01 MTR_3g464570 30.571 350 218 12 86 429 42 372 2.59e-40 148
MsG0580025831.01.T01 MTR_3g070900 30.411 365 207 15 87 429 106 445 1.49e-39 148
MsG0580025831.01.T01 MTR_8g463790 28.962 366 218 12 87 435 71 411 2.69e-39 146
MsG0580025831.01.T01 MTR_4g014440 29.526 359 220 13 87 429 62 403 3.48e-39 146
MsG0580025831.01.T01 MTR_3g035830 30.058 346 209 10 86 429 30 344 5.55e-39 144
MsG0580025831.01.T01 MTR_3g021090 31.519 349 200 14 86 429 30 344 1.91e-38 144
MsG0580025831.01.T01 MTR_3g021060 30.484 351 199 13 87 429 46 359 2.25e-38 143
MsG0580025831.01.T01 MTR_1g050448 30.278 360 219 10 78 433 44 375 1.34e-37 141
MsG0580025831.01.T01 MTR_4g125060 28.608 388 209 13 75 430 117 468 4.38e-37 141
MsG0580025831.01.T01 MTR_1g112210 29.670 364 191 15 87 429 59 378 4.56e-37 139
MsG0580025831.01.T01 MTR_5g083260 27.610 431 268 14 19 430 16 421 5.39e-37 140
MsG0580025831.01.T01 MTR_4g125060 28.421 380 207 12 80 430 164 507 8.03e-37 141
MsG0580025831.01.T01 MTR_4g125060 28.421 380 207 12 80 430 193 536 8.64e-37 142
MsG0580025831.01.T01 MTR_3g090120 27.791 421 250 17 30 433 76 459 4.27e-36 139
MsG0580025831.01.T01 MTR_1g112210 29.558 362 181 15 87 429 59 365 4.46e-36 137
MsG0580025831.01.T01 MTR_2g091175 30.347 346 205 12 87 429 50 362 1.01e-35 135
MsG0580025831.01.T01 MTR_7g019140 41.104 163 86 3 19 178 15 170 2.85e-35 129
MsG0580025831.01.T01 MTR_8g093870 26.234 385 224 12 74 430 117 469 1.70e-34 134
MsG0580025831.01.T01 MTR_3g049330 29.558 362 208 11 78 429 22 346 2.51e-34 131
MsG0580025831.01.T01 MTR_4g088030 27.095 358 225 10 73 429 24 346 2.72e-34 131
MsG0580025831.01.T01 MTR_6g016240 29.620 368 214 15 86 431 70 414 4.94e-34 132
MsG0580025831.01.T01 MTR_4g079700 27.494 411 237 13 29 433 8 363 8.05e-34 130
MsG0580025831.01.T01 MTR_3g071650 27.918 437 251 19 27 430 22 427 8.48e-34 132
MsG0580025831.01.T01 MTR_4g087730 28.099 363 214 12 75 429 35 358 1.68e-33 129
MsG0580025831.01.T01 MTR_5g082880 28.689 366 215 15 87 429 165 507 2.81e-33 131
MsG0580025831.01.T01 MTR_2g073500 29.915 351 200 14 87 429 29 341 3.82e-33 128
MsG0580025831.01.T01 MTR_2g073510 29.972 357 204 14 87 433 33 353 2.21e-32 126
MsG0580025831.01.T01 MTR_4g014430 27.926 376 227 14 76 433 97 446 3.99e-32 127
MsG0580025831.01.T01 MTR_2g015720 29.060 351 217 12 84 433 39 358 1.17e-31 124
MsG0580025831.01.T01 MTR_1g111010 28.646 384 223 16 57 433 2 341 6.73e-31 122
MsG0580025831.01.T01 MTR_5g033800 30.385 260 149 8 78 322 137 379 3.95e-30 120
MsG0580025831.01.T01 MTR_5g034810 29.609 358 215 13 76 429 24 348 1.15e-29 119
MsG0580025831.01.T01 MTR_4g092830 25.798 376 219 14 87 433 48 392 5.89e-27 112
MsG0580025831.01.T01 MTR_7g070060 33.514 185 116 4 80 259 57 239 1.76e-26 108
MsG0580025831.01.T01 MTR_4g102570 38.462 156 83 4 203 352 2 150 8.75e-26 102
MsG0580025831.01.T01 MTR_7g117160 32.877 219 89 7 214 432 29 189 1.17e-22 95.5
MsG0580025831.01.T01 MTR_7g070060 27.124 306 191 13 139 429 15 303 2.08e-22 97.4
MsG0580025831.01.T01 MTR_7g019130 35.938 128 75 3 205 328 1 125 4.35e-22 92.4
MsG0580025831.01.T01 MTR_3g464570 26.667 285 184 12 151 429 3 268 8.89e-19 86.3
MsG0580025831.01.T01 MTR_0929s0010 57.143 49 21 0 87 135 79 127 1.93e-15 73.2
MsG0580025831.01.T01 MTR_0929s0020 34.524 84 52 1 205 285 1 84 9.16e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025831.01.T01 AT1G70230 57.041 419 163 6 20 437 13 415 1.11e-177 503
MsG0580025831.01.T01 AT1G01430 39.583 432 236 8 30 441 29 455 1.68e-105 321
MsG0580025831.01.T01 AT4G01080 44.751 362 183 10 75 431 79 428 6.63e-103 313
MsG0580025831.01.T01 AT5G15890 41.117 394 214 8 42 434 147 523 4.61e-102 314
MsG0580025831.01.T01 AT3G02440 42.938 354 185 7 87 434 127 469 1.02e-99 306
MsG0580025831.01.T01 AT3G02440 42.938 354 185 7 87 434 135 477 1.26e-99 306
MsG0580025831.01.T01 AT4G23790 38.795 415 242 8 27 433 17 427 6.50e-99 303
MsG0580025831.01.T01 AT5G15900 40.933 386 205 11 66 438 39 414 7.24e-97 297
MsG0580025831.01.T01 AT5G15900 40.933 386 205 11 66 438 58 433 8.40e-97 298
MsG0580025831.01.T01 AT3G28150 40.845 355 196 7 85 435 68 412 1.89e-93 288
MsG0580025831.01.T01 AT4G11090 40.476 378 213 8 62 433 54 425 1.56e-92 286
MsG0580025831.01.T01 AT4G25360 35.733 389 229 9 59 435 147 526 2.92e-73 239
MsG0580025831.01.T01 AT4G25360 35.733 389 229 9 59 435 147 526 2.92e-73 239
MsG0580025831.01.T01 AT5G51640 34.795 365 210 11 87 435 138 490 5.43e-65 217
MsG0580025831.01.T01 AT5G19160 33.152 368 225 10 87 443 99 456 1.47e-54 189
MsG0580025831.01.T01 AT3G12060 31.378 392 230 13 54 429 163 531 2.11e-50 179
MsG0580025831.01.T01 AT3G06080 34.903 361 211 13 87 437 103 449 6.61e-50 176
MsG0580025831.01.T01 AT5G06700 33.690 374 208 16 87 443 255 605 1.28e-49 178
MsG0580025831.01.T01 AT5G64020 30.978 368 213 12 86 430 56 405 1.95e-47 168
MsG0580025831.01.T01 AT1G48880 34.530 362 179 17 87 430 115 436 2.57e-47 169
MsG0580025831.01.T01 AT5G20590 33.240 358 201 14 87 429 141 475 7.25e-47 168
MsG0580025831.01.T01 AT3G11570 32.849 344 188 14 103 429 98 415 7.02e-45 162
MsG0580025831.01.T01 AT5G06230 32.507 363 208 14 84 429 18 360 9.90e-45 160
MsG0580025831.01.T01 AT5G06230 32.507 363 208 14 84 429 59 401 1.88e-44 160
MsG0580025831.01.T01 AT5G49340 30.412 388 240 14 57 433 73 441 4.83e-44 160
MsG0580025831.01.T01 AT2G30010 31.429 385 230 13 63 433 32 396 5.10e-44 159
MsG0580025831.01.T01 AT2G30010 31.429 385 230 13 63 433 75 439 1.05e-43 159
MsG0580025831.01.T01 AT3G62390 31.148 366 206 13 87 435 138 474 1.13e-42 157
MsG0580025831.01.T01 AT2G40160 28.638 426 250 18 30 429 22 419 2.55e-42 155
MsG0580025831.01.T01 AT2G40160 28.638 426 250 18 30 429 22 419 2.70e-42 155
MsG0580025831.01.T01 AT1G73140 31.978 369 208 17 78 429 59 401 2.01e-41 152
MsG0580025831.01.T01 AT2G14530 28.993 407 225 15 58 433 33 406 3.92e-41 151
MsG0580025831.01.T01 AT5G19160 36.066 244 141 6 87 322 99 335 1.74e-40 148
MsG0580025831.01.T01 AT5G58600 29.834 362 214 12 86 433 65 400 5.00e-40 148
MsG0580025831.01.T01 AT1G60790 29.268 369 216 13 87 434 190 534 1.15e-39 150
MsG0580025831.01.T01 AT2G37720 28.261 368 219 11 82 430 138 479 3.13e-38 145
MsG0580025831.01.T01 AT2G40320 28.809 361 220 14 86 430 80 419 3.83e-38 144
MsG0580025831.01.T01 AT2G42570 30.484 351 207 12 87 433 47 364 5.92e-38 142
MsG0580025831.01.T01 AT3G11030 29.050 358 222 13 87 430 106 445 7.00e-38 143
MsG0580025831.01.T01 AT3G55990 30.471 361 212 14 87 429 140 479 2.86e-37 142
MsG0580025831.01.T01 AT5G01360 28.610 367 222 13 82 430 31 375 3.39e-37 140
MsG0580025831.01.T01 AT3G14850 29.944 354 208 11 87 433 34 354 5.65e-37 139
MsG0580025831.01.T01 AT2G38320 28.490 351 234 10 87 429 59 400 6.78e-37 140
MsG0580025831.01.T01 AT5G01360 28.302 371 218 15 82 430 87 431 1.01e-36 140
MsG0580025831.01.T01 AT2G34070 28.329 353 219 12 84 433 62 383 1.44e-36 138
MsG0580025831.01.T01 AT2G40150 28.866 388 235 15 64 429 48 416 1.59e-36 139
MsG0580025831.01.T01 AT2G31110 29.191 346 210 12 87 429 45 358 3.00e-36 137
MsG0580025831.01.T01 AT2G30900 29.513 349 210 10 87 429 43 361 3.97e-36 137
MsG0580025831.01.T01 AT1G78710 30.423 355 203 13 87 433 38 356 6.52e-35 133
MsG0580025831.01.T01 AT3G06080 35.772 246 141 8 87 323 103 340 1.81e-34 132
MsG0580025831.01.T01 AT5G64470 27.926 376 220 15 80 433 48 394 4.62e-34 132
MsG0580025831.01.T01 AT1G29050 29.023 348 216 13 87 433 61 378 1.00e-33 130
MsG0580025831.01.T01 AT5G64470 27.749 382 219 16 80 433 48 400 3.18e-33 129
MsG0580025831.01.T01 AT5G20680 27.151 372 216 13 86 433 117 457 9.57e-33 129
MsG0580025831.01.T01 AT5G20680 27.151 372 216 13 86 433 193 533 1.06e-32 130
MsG0580025831.01.T01 AT5G20680 27.151 372 216 13 86 433 211 551 1.09e-32 130
MsG0580025831.01.T01 AT5G20680 27.151 372 216 13 86 433 211 551 1.09e-32 130
MsG0580025831.01.T01 AT5G20680 27.151 372 216 13 86 433 211 551 1.09e-32 130
MsG0580025831.01.T01 AT5G20680 27.151 372 216 13 86 433 211 551 1.09e-32 130
MsG0580025831.01.T01 AT3G54260 29.494 356 210 14 87 433 51 374 4.82e-32 125
MsG0580025831.01.T01 AT2G40160 28.990 307 187 10 30 322 22 311 7.26e-32 124
MsG0580025831.01.T01 AT5G01620 27.078 373 225 14 81 433 104 449 9.37e-32 126
MsG0580025831.01.T01 AT5G01620 27.078 373 225 14 81 433 104 449 9.37e-32 126
MsG0580025831.01.T01 AT2G38320 28.125 320 214 9 117 429 7 317 9.83e-32 124
MsG0580025831.01.T01 AT5G01620 27.078 373 225 14 81 433 112 457 1.19e-31 126
MsG0580025831.01.T01 AT5G20590 33.068 251 141 10 87 323 141 378 2.46e-31 124
MsG0580025831.01.T01 AT3G62390 32.377 244 152 7 87 324 138 374 7.06e-30 120
MsG0580025831.01.T01 AT5G06230 32.422 256 148 9 84 323 59 305 7.70e-29 115
MsG0580025831.01.T01 AT5G58600 31.950 241 143 8 86 321 65 289 4.19e-28 113
MsG0580025831.01.T01 AT2G30900 31.579 190 123 3 87 275 43 226 6.79e-25 103
MsG0580025831.01.T01 AT2G31110 32.759 174 110 3 87 260 45 211 8.86e-25 103
MsG0580025831.01.T01 AT1G78710 32.948 173 107 4 87 258 31 195 1.30e-20 92.4
MsG0580025831.01.T01 AT5G64470 26.761 284 174 12 80 344 48 316 1.11e-19 90.1
MsG0580025831.01.T01 AT5G64470 27.437 277 167 12 80 337 48 309 1.83e-19 89.4
MsG0580025831.01.T01 AT2G34070 27.338 278 172 13 158 434 2 250 9.74e-16 77.0
MsG0580025831.01.T01 AT3G14850 26.502 283 168 11 158 433 2 251 3.52e-14 72.8

Find 116 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCTTAATCATACTGAAAT+TGG 0.102828 5:-24849481 MsG0580025831.01.T01:CDS
TCCTATCAATTATGCTATTA+AGG 0.141791 5:+24849422 None:intergenic
AAATCATAATAAGTTGTATT+TGG 0.178202 5:-24849635 MsG0580025831.01.T01:CDS
AACCAAAATGGTCATGAAAA+TGG 0.179263 5:-24850444 None:intergenic
ACTCTTCACTCTTGAGTTCT+TGG 0.205568 5:+24848989 None:intergenic
CTTCTATAAGGCTTAGTCTT+TGG 0.224979 5:+24849310 None:intergenic
CAGATTCTGATTATCTTTAC+TGG 0.261322 5:-24849939 MsG0580025831.01.T01:CDS
GTGACTACTTCAATGGAAAA+TGG 0.280998 5:-24850047 MsG0580025831.01.T01:CDS
TCCTTCTCCCTACTTCTATA+AGG 0.286857 5:+24849298 None:intergenic
AAATACAACTTATTATGATT+TGG 0.294595 5:+24849637 None:intergenic
TATGGATATTGCATTGAAAT+AGG 0.300177 5:-24848906 MsG0580025831.01.T01:three_prime_UTR
AGGTGTAGAGTGGGAGATAA+TGG 0.303368 5:+24850262 None:intergenic
TTTGTTATACCTTCATATTA+TGG 0.312763 5:-24848924 MsG0580025831.01.T01:three_prime_UTR
AGGAAATAAAGGTAGTAAAA+TGG 0.324311 5:-24849398 MsG0580025831.01.T01:CDS
TTCTGGAGATTATGAAGAAA+TGG 0.334403 5:-24849021 MsG0580025831.01.T01:CDS
TGAAAGATGGGCAAAGGATA+TGG 0.342119 5:-24849602 MsG0580025831.01.T01:CDS
CTGATTATCTTTACTGGAGA+TGG 0.369650 5:-24849933 MsG0580025831.01.T01:CDS
AATGGAAAAGGGTACATATA+AGG 0.371725 5:+24849127 None:intergenic
TGTGTTCATTGGTGTTTGCC+TGG 0.372187 5:-24849070 MsG0580025831.01.T01:CDS
ATGAATTGAAGAAAAGTGTT+AGG 0.375554 5:+24849877 None:intergenic
AAGGTGAAAAGTAGTGAATT+TGG 0.378681 5:-24849217 MsG0580025831.01.T01:CDS
TTGTGCTTTCAATTGGTCAT+TGG 0.379558 5:-24849564 MsG0580025831.01.T01:CDS
ATTCATTGAAAACAAACATA+TGG 0.383919 5:-24849860 MsG0580025831.01.T01:CDS
ATTGATAGGAGAGGAAATAA+AGG 0.397784 5:-24849409 MsG0580025831.01.T01:CDS
GTCTTAATCATACTGAAATT+GGG 0.403211 5:-24849480 MsG0580025831.01.T01:CDS
TCAACACCTTGAACAAGAAA+AGG 0.403244 5:+24849676 None:intergenic
CCTATCAATTATGCTATTAA+GGG 0.403903 5:+24849423 None:intergenic
GGAAGCTCAGATTCAGTAGA+AGG 0.404741 5:+24850299 None:intergenic
TCTGGAGATTATGAAGAAAT+GGG 0.408121 5:-24849020 MsG0580025831.01.T01:CDS
CAGTAAAGATAATCAGAATC+TGG 0.424934 5:+24849940 None:intergenic
TAAAAGAAGGAGGTGTAGAG+TGG 0.427411 5:+24850252 None:intergenic
TGCAAAATGATTGTGTTCAT+TGG 0.427697 5:-24849081 MsG0580025831.01.T01:CDS
ACCAAAATGGTCATGAAAAT+GGG 0.432239 5:-24850443 None:intergenic
AGACTAAGCCTTATAGAAGT+AGG 0.435118 5:-24849306 MsG0580025831.01.T01:CDS
ATTTGGAGGGGTTAGGTTTG+AGG 0.439421 5:-24849200 MsG0580025831.01.T01:CDS
GGCAAAGGATATGGAAAAGA+TGG 0.440553 5:-24849593 MsG0580025831.01.T01:CDS
ACCCCATTAGCAAATGGAAA+AGG 0.442392 5:+24849115 None:intergenic
CCCACATGTGACTACTTCAA+TGG 0.443622 5:-24850054 MsG0580025831.01.T01:CDS
TTAGGTTGTCATTATTGTGT+TGG 0.447247 5:-24849502 MsG0580025831.01.T01:CDS
GAGTGGAATTGATCTTTGAA+TGG 0.450724 5:+24850410 None:intergenic
CATGTTGTTCCATTGTATAA+AGG 0.454390 5:+24850006 None:intergenic
TGGATCTTGTTGATGAAAGA+TGG 0.457453 5:-24849615 MsG0580025831.01.T01:CDS
CAACACCTTGAACAAGAAAA+GGG 0.457580 5:+24849677 None:intergenic
TTCTTGTTCTATAATAGAAA+AGG 0.461052 5:-24848810 MsG0580025831.01.T01:three_prime_UTR
TAAGGACCTGGATGACCATC+TGG 0.461971 5:+24849145 None:intergenic
GAACTCAAGAGTGAAGAGTT+AGG 0.464387 5:-24848985 MsG0580025831.01.T01:exon
ATCTCATTCCATGTGTCTAT+AGG 0.470243 5:+24849046 None:intergenic
AGATTCAGTAGAAGGAAGAA+AGG 0.471233 5:+24850307 None:intergenic
TTGAGACCAGATGGTCATCC+AGG 0.479603 5:-24849151 MsG0580025831.01.T01:CDS
TAGAAGGAAGAAAGGGAAGA+GGG 0.484026 5:+24850315 None:intergenic
TATAAACTAAGAAGAGCAAT+GGG 0.491035 5:+24850344 None:intergenic
CCACTAGTGTAAACAAGATC+AGG 0.492606 5:+24849766 None:intergenic
AGAGGCAATTTCATTGAGGA+TGG 0.495421 5:-24848858 MsG0580025831.01.T01:three_prime_UTR
GGAGAAGGAACTTGAAGGAA+TGG 0.495842 5:-24849284 MsG0580025831.01.T01:CDS
CTTGGAAGTTTACATTCACT+TGG 0.499857 5:+24849907 None:intergenic
TTCCATGTGTCTATAGGTCC+AGG 0.501440 5:+24849052 None:intergenic
TTGTTGGTGATTCTATGGCT+AGG 0.502299 5:-24849834 MsG0580025831.01.T01:CDS
AAAAGAAGGAGGTGTAGAGT+GGG 0.507492 5:+24850253 None:intergenic
CCCTTAATAGCATAATTGAT+AGG 0.509822 5:-24849423 MsG0580025831.01.T01:CDS
GATTCAGTAGAAGGAAGAAA+GGG 0.509845 5:+24850308 None:intergenic
TGAAGAAGTTGAAGATGCTA+AGG 0.511290 5:-24849236 MsG0580025831.01.T01:CDS
TCTATAATAGAAAAGGAGTA+AGG 0.512722 5:-24848803 MsG0580025831.01.T01:three_prime_UTR
AGTAGGGAGAAGGAACTTGA+AGG 0.514231 5:-24849289 MsG0580025831.01.T01:CDS
GACTAAGCCTTATAGAAGTA+GGG 0.514785 5:-24849305 MsG0580025831.01.T01:CDS
CCATTGAAGTAGTCACATGT+GGG 0.515482 5:+24850054 None:intergenic
AGGATATTAAGTTTAGAAAG+TGG 0.516473 5:-24849741 MsG0580025831.01.T01:CDS
CATAAACGTACCTGAAGGAG+GGG 0.517380 5:+24850220 None:intergenic
AGAAGAGCATAAAGAGTCCA+AGG 0.521608 5:+24850368 None:intergenic
CCCCATTAGCAAATGGAAAA+GGG 0.522058 5:+24849116 None:intergenic
GTAGTGAATTTGGAGGGGTT+AGG 0.522463 5:-24849207 MsG0580025831.01.T01:CDS
GTAGAAGGAAGAAAGGGAAG+AGG 0.526273 5:+24850314 None:intergenic
CCTGATCTTGTTTACACTAG+TGG 0.531783 5:-24849766 MsG0580025831.01.T01:CDS
CAAGTGAATGTAAACTTCCA+AGG 0.533304 5:-24849906 MsG0580025831.01.T01:CDS
AAAGTGTTAGGCTCAAACCT+TGG 0.534233 5:+24849889 None:intergenic
GGATCTTGTTGATGAAAGAT+GGG 0.537727 5:-24849614 MsG0580025831.01.T01:CDS
ATGCAAGTGTGTCTGTGTAT+TGG 0.538334 5:-24849702 MsG0580025831.01.T01:CDS
ATGCAATATCCATAATATGA+AGG 0.540080 5:+24848915 None:intergenic
AGTGGAATTGATCTTTGAAT+GGG 0.540788 5:+24850411 None:intergenic
TCCATTGAAGTAGTCACATG+TGG 0.548479 5:+24850053 None:intergenic
TGAAAAGTAGTGAATTTGGA+GGG 0.552481 5:-24849213 MsG0580025831.01.T01:CDS
TCTGGAACCCCATTAGCAAA+TGG 0.557935 5:+24849109 None:intergenic
TCACCTTCGTAGTAAACTGC+AGG 0.557945 5:+24849532 None:intergenic
ATAATGGTAGTAGTTGAATG+AGG 0.558338 5:+24850278 None:intergenic
GTGTTGAGAAATCAAATGAC+GGG 0.563717 5:-24849660 MsG0580025831.01.T01:CDS
ACATGTGGAACAATCAAAGA+AGG 0.566099 5:-24849988 MsG0580025831.01.T01:CDS
CATTCACCTTCAAAATGAGC+TGG 0.568324 5:+24849352 None:intergenic
TTATACAATGGAACAACATG+TGG 0.569031 5:-24850003 MsG0580025831.01.T01:CDS
TTGCCATTGTTGAGACCAGA+TGG 0.574460 5:-24849160 MsG0580025831.01.T01:CDS
ATATAAACTAAGAAGAGCAA+TGG 0.579490 5:+24850343 None:intergenic
GCCTTATAGAAGTAGGGAGA+AGG 0.580965 5:-24849299 MsG0580025831.01.T01:CDS
CATCCTGCAGTTTACTACGA+AGG 0.583567 5:-24849535 MsG0580025831.01.T01:CDS
TGCATAAACGTACCTGAAGG+AGG 0.585332 5:+24850218 None:intergenic
GTGAAAAGTAGTGAATTTGG+AGG 0.590767 5:-24849214 MsG0580025831.01.T01:CDS
TGTTGATGAAAGATGGGCAA+AGG 0.591875 5:-24849608 MsG0580025831.01.T01:CDS
GAAAAGTAGTGAATTTGGAG+GGG 0.593375 5:-24849212 MsG0580025831.01.T01:CDS
GAACAGAGGCAATTTCATTG+AGG 0.594154 5:-24848862 MsG0580025831.01.T01:three_prime_UTR
ATAAAGGTCCTCTTCTATCA+CGG 0.598847 5:+24850022 None:intergenic
GAAGGTGAATGGGATAAAGC+TGG 0.607431 5:-24849340 MsG0580025831.01.T01:CDS
TAGTGAGATGAGGAAAATTG+AGG 0.613129 5:-24849260 MsG0580025831.01.T01:CDS
AAATGGATCCGTGATAGAAG+AGG 0.613784 5:-24850030 MsG0580025831.01.T01:CDS
GGTGTTGAGAAATCAAATGA+CGG 0.613855 5:-24849661 MsG0580025831.01.T01:CDS
GGTGTAGAAGTAAAAGAAGG+AGG 0.614256 5:+24850242 None:intergenic
AGAAGAGGACCTTTATACAA+TGG 0.622543 5:-24850015 MsG0580025831.01.T01:CDS
GGACAAAATTGTATCAAACA+TGG 0.625532 5:-24849967 MsG0580025831.01.T01:CDS
AATAGCATAATTGATAGGAG+AGG 0.629684 5:-24849418 MsG0580025831.01.T01:CDS
GCATAAACGTACCTGAAGGA+GGG 0.633056 5:+24850219 None:intergenic
AAAGGGTACATATAAGGACC+TGG 0.633154 5:+24849133 None:intergenic
TGACCATCTGGTCTCAACAA+TGG 0.633379 5:+24849157 None:intergenic
CATTGAAGTAGTCACATGTG+GGG 0.636248 5:+24850055 None:intergenic
AAGGAATGGATAGTGAGATG+AGG 0.645343 5:-24849270 MsG0580025831.01.T01:CDS
AACACCTTGAACAAGAAAAG+GGG 0.652940 5:+24849678 None:intergenic
TCTTGTTTACACTAGTGGTG+AGG 0.661471 5:-24849761 MsG0580025831.01.T01:CDS
ATTGAAGTAGTCACATGTGG+GGG 0.680101 5:+24850056 None:intergenic
GGAGATTATGAAGAAATGGG+AGG 0.680620 5:-24849017 MsG0580025831.01.T01:CDS
TGCCTGGACCTATAGACACA+TGG 0.689465 5:-24849054 MsG0580025831.01.T01:CDS
ATAAACGTACCTGAAGGAGG+GGG 0.706780 5:+24850221 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTAAAGATGTTTTATAT+TGG - Chr5:24850437-24850456 MsG0580025831.01.T01:CDS 10.0%
!! AAATACAACTTATTATGATT+TGG + Chr5:24849573-24849592 None:intergenic 15.0%
!! AAATCATAATAAGTTGTATT+TGG - Chr5:24849572-24849591 MsG0580025831.01.T01:CDS 15.0%
!!! AAAAATGATGTTTTTACAAA+TGG - Chr5:24849027-24849046 MsG0580025831.01.T01:CDS 15.0%
!!! AAAATGATGTTTTTACAAAT+GGG - Chr5:24849028-24849047 MsG0580025831.01.T01:CDS 15.0%
!! ATTCATTGAAAACAAACATA+TGG - Chr5:24849347-24849366 MsG0580025831.01.T01:CDS 20.0%
!! TTCTTGTTCTATAATAGAAA+AGG - Chr5:24850397-24850416 MsG0580025831.01.T01:CDS 20.0%
!!! AATTTTCTTTATTGTAGAGA+AGG - Chr5:24849113-24849132 MsG0580025831.01.T01:CDS 20.0%
!!! TTTGTTATACCTTCATATTA+TGG - Chr5:24850283-24850302 MsG0580025831.01.T01:CDS 20.0%
! AGGAAATAAAGGTAGTAAAA+TGG - Chr5:24849809-24849828 MsG0580025831.01.T01:CDS 25.0%
! AGGATATTAAGTTTAGAAAG+TGG - Chr5:24849466-24849485 MsG0580025831.01.T01:CDS 25.0%
! ATATAAACTAAGAAGAGCAA+TGG + Chr5:24848867-24848886 None:intergenic 25.0%
! ATGCAATATCCATAATATGA+AGG + Chr5:24850295-24850314 None:intergenic 25.0%
! CCTATCAATTATGCTATTAA+GGG + Chr5:24849787-24849806 None:intergenic 25.0%
! GTCTTAATCATACTGAAATT+GGG - Chr5:24849727-24849746 MsG0580025831.01.T01:CDS 25.0%
! TATAAACTAAGAAGAGCAAT+GGG + Chr5:24848866-24848885 None:intergenic 25.0%
! TATGGATATTGCATTGAAAT+AGG - Chr5:24850301-24850320 MsG0580025831.01.T01:CDS 25.0%
! TCCTATCAATTATGCTATTA+AGG + Chr5:24849788-24849807 None:intergenic 25.0%
! TCTATAATAGAAAAGGAGTA+AGG - Chr5:24850404-24850423 MsG0580025831.01.T01:CDS 25.0%
!! GATTTTATTGTGCTTTCAAT+TGG - Chr5:24849636-24849655 MsG0580025831.01.T01:CDS 25.0%
!! GTTTTATGATGTATTGAGAA+AGG - Chr5:24849749-24849768 MsG0580025831.01.T01:CDS 25.0%
!!! ATGAATTGAAGAAAAGTGTT+AGG + Chr5:24849333-24849352 None:intergenic 25.0%
AAGGTGAAAAGTAGTGAATT+TGG - Chr5:24849990-24850009 MsG0580025831.01.T01:CDS 30.0%
AATAGCATAATTGATAGGAG+AGG - Chr5:24849789-24849808 MsG0580025831.01.T01:CDS 30.0%
AGTGGAATTGATCTTTGAAT+GGG + Chr5:24848799-24848818 None:intergenic 30.0%
ATAATGGTAGTAGTTGAATG+AGG + Chr5:24848932-24848951 None:intergenic 30.0%
ATTGATAGGAGAGGAAATAA+AGG - Chr5:24849798-24849817 MsG0580025831.01.T01:CDS 30.0%
CAGATTCTGATTATCTTTAC+TGG - Chr5:24849268-24849287 MsG0580025831.01.T01:CDS 30.0%
CAGTAAAGATAATCAGAATC+TGG + Chr5:24849270-24849289 None:intergenic 30.0%
CATGTTGTTCCATTGTATAA+AGG + Chr5:24849204-24849223 None:intergenic 30.0%
CCCTTAATAGCATAATTGAT+AGG - Chr5:24849784-24849803 MsG0580025831.01.T01:CDS 30.0%
GGACAAAATTGTATCAAACA+TGG - Chr5:24849240-24849259 MsG0580025831.01.T01:CDS 30.0%
GGTCTTAATCATACTGAAAT+TGG - Chr5:24849726-24849745 MsG0580025831.01.T01:CDS 30.0%
TCTGGAGATTATGAAGAAAT+GGG - Chr5:24850187-24850206 MsG0580025831.01.T01:intron 30.0%
TGAAAAGTAGTGAATTTGGA+GGG - Chr5:24849994-24850013 MsG0580025831.01.T01:CDS 30.0%
TGCAAAATGATTGTGTTCAT+TGG - Chr5:24850126-24850145 MsG0580025831.01.T01:intron 30.0%
TTATACAATGGAACAACATG+TGG - Chr5:24849204-24849223 MsG0580025831.01.T01:CDS 30.0%
! AACAAACATATGGCTTTTGT+TGG - Chr5:24849357-24849376 MsG0580025831.01.T01:CDS 30.0%
! AATGGAAAAGGGTACATATA+AGG + Chr5:24850083-24850102 None:intergenic 30.0%
! TTAGGTTGTCATTATTGTGT+TGG - Chr5:24849705-24849724 MsG0580025831.01.T01:CDS 30.0%
! TTCTGGAGATTATGAAGAAA+TGG - Chr5:24850186-24850205 MsG0580025831.01.T01:intron 30.0%
!! TCACCAAAAAACTTTTTCCT+TGG - Chr5:24848822-24848841 MsG0580025831.01.T01:three_prime_UTR 30.0%
!!! AGGAAAAAGTTTTTTGGTGA+TGG + Chr5:24848822-24848841 None:intergenic 30.0%
!!! AGTCCAAGGAAAAAGTTTTT+TGG + Chr5:24848828-24848847 None:intergenic 30.0%
!!! TTGTTTTTGATAGAGAACAG+AGG - Chr5:24850331-24850350 MsG0580025831.01.T01:CDS 30.0%
AACACCTTGAACAAGAAAAG+GGG + Chr5:24849532-24849551 None:intergenic 35.0%
ACATGTGGAACAATCAAAGA+AGG - Chr5:24849219-24849238 MsG0580025831.01.T01:CDS 35.0%
AGAAGAGGACCTTTATACAA+TGG - Chr5:24849192-24849211 MsG0580025831.01.T01:CDS 35.0%
AGATTCAGTAGAAGGAAGAA+AGG + Chr5:24848903-24848922 None:intergenic 35.0%
ATAAAGGTCCTCTTCTATCA+CGG + Chr5:24849188-24849207 None:intergenic 35.0%
ATCTCATTCCATGTGTCTAT+AGG + Chr5:24850164-24850183 None:intergenic 35.0%
CAACACCTTGAACAAGAAAA+GGG + Chr5:24849533-24849552 None:intergenic 35.0%
CAAGTGAATGTAAACTTCCA+AGG - Chr5:24849301-24849320 MsG0580025831.01.T01:CDS 35.0%
CTGATTATCTTTACTGGAGA+TGG - Chr5:24849274-24849293 MsG0580025831.01.T01:CDS 35.0%
CTTGGAAGTTTACATTCACT+TGG + Chr5:24849303-24849322 None:intergenic 35.0%
GAAAAGTAGTGAATTTGGAG+GGG - Chr5:24849995-24850014 MsG0580025831.01.T01:CDS 35.0%
GAGTGGAATTGATCTTTGAA+TGG + Chr5:24848800-24848819 None:intergenic 35.0%
GATTCAGTAGAAGGAAGAAA+GGG + Chr5:24848902-24848921 None:intergenic 35.0%
GTGAAAAGTAGTGAATTTGG+AGG - Chr5:24849993-24850012 MsG0580025831.01.T01:CDS 35.0%
GTGACTACTTCAATGGAAAA+TGG - Chr5:24849160-24849179 MsG0580025831.01.T01:CDS 35.0%
GTGTTGAGAAATCAAATGAC+GGG - Chr5:24849547-24849566 MsG0580025831.01.T01:CDS 35.0%
TAGTGAGATGAGGAAAATTG+AGG - Chr5:24849947-24849966 MsG0580025831.01.T01:CDS 35.0%
TCAACACCTTGAACAAGAAA+AGG + Chr5:24849534-24849553 None:intergenic 35.0%
TGAAGAAGTTGAAGATGCTA+AGG - Chr5:24849971-24849990 MsG0580025831.01.T01:CDS 35.0%
TTGTGCTTTCAATTGGTCAT+TGG - Chr5:24849643-24849662 MsG0580025831.01.T01:CDS 35.0%
! AGACTAAGCCTTATAGAAGT+AGG - Chr5:24849901-24849920 MsG0580025831.01.T01:CDS 35.0%
! CACATGGAATGAGATTTTTC+TGG - Chr5:24850169-24850188 MsG0580025831.01.T01:intron 35.0%
! CTTCTATAAGGCTTAGTCTT+TGG + Chr5:24849900-24849919 None:intergenic 35.0%
! GACTAAGCCTTATAGAAGTA+GGG - Chr5:24849902-24849921 MsG0580025831.01.T01:CDS 35.0%
! GGATCTTGTTGATGAAAGAT+GGG - Chr5:24849593-24849612 MsG0580025831.01.T01:CDS 35.0%
! GGTGTTGAGAAATCAAATGA+CGG - Chr5:24849546-24849565 MsG0580025831.01.T01:CDS 35.0%
! TACGAAGGTGATTCAGTTTT+AGG - Chr5:24849687-24849706 MsG0580025831.01.T01:CDS 35.0%
! TGGATCTTGTTGATGAAAGA+TGG - Chr5:24849592-24849611 MsG0580025831.01.T01:CDS 35.0%
! TTTTCACCAGCTCATTTTGA+AGG - Chr5:24849849-24849868 MsG0580025831.01.T01:CDS 35.0%
!! ACCCTTTTCCATTTGCTAAT+GGG - Chr5:24850090-24850109 MsG0580025831.01.T01:intron 35.0%
!! AGCTCATTTTGAAGGTGAAT+GGG - Chr5:24849857-24849876 MsG0580025831.01.T01:CDS 35.0%
!! TACCCTTTTCCATTTGCTAA+TGG - Chr5:24850089-24850108 MsG0580025831.01.T01:intron 35.0%
!!! AAAGTTTTTTGGTGATGGAG+TGG + Chr5:24848817-24848836 None:intergenic 35.0%
AAATGGATCCGTGATAGAAG+AGG - Chr5:24849177-24849196 MsG0580025831.01.T01:CDS 40.0%
AAGGAATGGATAGTGAGATG+AGG - Chr5:24849937-24849956 MsG0580025831.01.T01:CDS 40.0%
ACCCCATTAGCAAATGGAAA+AGG + Chr5:24850095-24850114 None:intergenic 40.0%
ACTCTTCACTCTTGAGTTCT+TGG + Chr5:24850221-24850240 None:intergenic 40.0%
AGAGGCAATTTCATTGAGGA+TGG - Chr5:24850349-24850368 MsG0580025831.01.T01:CDS 40.0%
ATGCAAGTGTGTCTGTGTAT+TGG - Chr5:24849505-24849524 MsG0580025831.01.T01:CDS 40.0%
ATTGAAGTAGTCACATGTGG+GGG + Chr5:24849154-24849173 None:intergenic 40.0%
CATTCACCTTCAAAATGAGC+TGG + Chr5:24849858-24849877 None:intergenic 40.0%
CATTGAAGTAGTCACATGTG+GGG + Chr5:24849155-24849174 None:intergenic 40.0%
CCACTAGTGTAAACAAGATC+AGG + Chr5:24849444-24849463 None:intergenic 40.0%
CCATTGAAGTAGTCACATGT+GGG + Chr5:24849156-24849175 None:intergenic 40.0%
CCCCATTAGCAAATGGAAAA+GGG + Chr5:24850094-24850113 None:intergenic 40.0%
GAACAGAGGCAATTTCATTG+AGG - Chr5:24850345-24850364 MsG0580025831.01.T01:CDS 40.0%
GAACTCAAGAGTGAAGAGTT+AGG - Chr5:24850222-24850241 MsG0580025831.01.T01:intron 40.0%
GGAGATTATGAAGAAATGGG+AGG - Chr5:24850190-24850209 MsG0580025831.01.T01:intron 40.0%
GGCAAAGGATATGGAAAAGA+TGG - Chr5:24849614-24849633 MsG0580025831.01.T01:CDS 40.0%
GGTGTAGAAGTAAAAGAAGG+AGG + Chr5:24848968-24848987 None:intergenic 40.0%
GTATGCATAAACGTACCTGA+AGG + Chr5:24848995-24849014 None:intergenic 40.0%
TAGAAGGAAGAAAGGGAAGA+GGG + Chr5:24848895-24848914 None:intergenic 40.0%
TCCATTGAAGTAGTCACATG+TGG + Chr5:24849157-24849176 None:intergenic 40.0%
TCCTTCTCCCTACTTCTATA+AGG + Chr5:24849912-24849931 None:intergenic 40.0%
TGAAAGATGGGCAAAGGATA+TGG - Chr5:24849605-24849624 MsG0580025831.01.T01:CDS 40.0%
! AAAAGAAGGAGGTGTAGAGT+GGG + Chr5:24848957-24848976 None:intergenic 40.0%
! AAAGGGTACATATAAGGACC+TGG + Chr5:24850077-24850096 None:intergenic 40.0%
! AAAGTGTTAGGCTCAAACCT+TGG + Chr5:24849321-24849340 None:intergenic 40.0%
! AGAAGAGCATAAAGAGTCCA+AGG + Chr5:24848842-24848861 None:intergenic 40.0%
! CCTGATCTTGTTTACACTAG+TGG - Chr5:24849441-24849460 MsG0580025831.01.T01:CDS 40.0%
! TAAAAGAAGGAGGTGTAGAG+TGG + Chr5:24848958-24848977 None:intergenic 40.0%
! TCTTGTTTACACTAGTGGTG+AGG - Chr5:24849446-24849465 MsG0580025831.01.T01:CDS 40.0%
! TGTTGATGAAAGATGGGCAA+AGG - Chr5:24849599-24849618 MsG0580025831.01.T01:CDS 40.0%
!! CAGCTCATTTTGAAGGTGAA+TGG - Chr5:24849856-24849875 MsG0580025831.01.T01:CDS 40.0%
!! CCCTTTTCCATTTGCTAATG+GGG - Chr5:24850091-24850110 MsG0580025831.01.T01:intron 40.0%
!! TTGTTGGTGATTCTATGGCT+AGG - Chr5:24849373-24849392 MsG0580025831.01.T01:CDS 40.0%
!!! GGCTTTTGTTGGTGATTCTA+TGG - Chr5:24849368-24849387 MsG0580025831.01.T01:CDS 40.0%
AGGTGTAGAGTGGGAGATAA+TGG + Chr5:24848948-24848967 None:intergenic 45.0%
AGTAGGGAGAAGGAACTTGA+AGG - Chr5:24849918-24849937 MsG0580025831.01.T01:CDS 45.0%
ATAAACGTACCTGAAGGAGG+GGG + Chr5:24848989-24849008 None:intergenic 45.0%
ATTTGGAGGGGTTAGGTTTG+AGG - Chr5:24850007-24850026 MsG0580025831.01.T01:CDS 45.0%
CATAAACGTACCTGAAGGAG+GGG + Chr5:24848990-24849009 None:intergenic 45.0%
CATCCTGCAGTTTACTACGA+AGG - Chr5:24849672-24849691 MsG0580025831.01.T01:CDS 45.0%
CCCACATGTGACTACTTCAA+TGG - Chr5:24849153-24849172 MsG0580025831.01.T01:CDS 45.0%
GAAGGTGAATGGGATAAAGC+TGG - Chr5:24849867-24849886 MsG0580025831.01.T01:CDS 45.0%
GCATAAACGTACCTGAAGGA+GGG + Chr5:24848991-24849010 None:intergenic 45.0%
GCCTTATAGAAGTAGGGAGA+AGG - Chr5:24849908-24849927 MsG0580025831.01.T01:CDS 45.0%
GGAAGCTCAGATTCAGTAGA+AGG + Chr5:24848911-24848930 None:intergenic 45.0%
GGAGAAGGAACTTGAAGGAA+TGG - Chr5:24849923-24849942 MsG0580025831.01.T01:CDS 45.0%
GTAGAAGGAAGAAAGGGAAG+AGG + Chr5:24848896-24848915 None:intergenic 45.0%
GTAGTGAATTTGGAGGGGTT+AGG - Chr5:24850000-24850019 MsG0580025831.01.T01:CDS 45.0%
TCACCTTCGTAGTAAACTGC+AGG + Chr5:24849678-24849697 None:intergenic 45.0%
TGACCATCTGGTCTCAACAA+TGG + Chr5:24850053-24850072 None:intergenic 45.0%
TGCATAAACGTACCTGAAGG+AGG + Chr5:24848992-24849011 None:intergenic 45.0%
TTCCATGTGTCTATAGGTCC+AGG + Chr5:24850158-24850177 None:intergenic 45.0%
TTGCCATTGTTGAGACCAGA+TGG - Chr5:24850047-24850066 MsG0580025831.01.T01:CDS 45.0%
! CAATCATTTTGCACACGCTC+TGG + Chr5:24850119-24850138 None:intergenic 45.0%
! GGGGGTGTAGAAGTAAAAGA+AGG + Chr5:24848971-24848990 None:intergenic 45.0%
! TCTGGAACCCCATTAGCAAA+TGG + Chr5:24850101-24850120 None:intergenic 45.0%
! TGGTCCCCTTTTCTTGTTCA+AGG - Chr5:24849525-24849544 MsG0580025831.01.T01:CDS 45.0%
! TGTGTTCATTGGTGTTTGCC+TGG - Chr5:24850137-24850156 MsG0580025831.01.T01:intron 45.0%
TAAGGACCTGGATGACCATC+TGG + Chr5:24850065-24850084 None:intergenic 50.0%
TGCCTGGACCTATAGACACA+TGG - Chr5:24850153-24850172 MsG0580025831.01.T01:intron 50.0%
TTGAGACCAGATGGTCATCC+AGG - Chr5:24850056-24850075 MsG0580025831.01.T01:CDS 50.0%
ACTTCTACACCCCCTCCTTC+AGG - Chr5:24848977-24848996 MsG0580025831.01.T01:exon 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 24848770 24850459 24848770 ID=MsG0580025831.01;Name=MsG0580025831.01
Chr5 mRNA 24848770 24850459 24848770 ID=MsG0580025831.01.T01;Parent=MsG0580025831.01;Name=MsG0580025831.01.T01;_AED=0.49;_eAED=0.49;_QI=0|1|0.5|1|1|1|2|224|444
Chr5 exon 24848770 24850099 24848770 ID=MsG0580025831.01.T01:exon:4003;Parent=MsG0580025831.01.T01
Chr5 exon 24850231 24850459 24850231 ID=MsG0580025831.01.T01:exon:4002;Parent=MsG0580025831.01.T01
Chr5 CDS 24850231 24850459 24850231 ID=MsG0580025831.01.T01:cds;Parent=MsG0580025831.01.T01
Chr5 CDS 24848994 24850099 24848994 ID=MsG0580025831.01.T01:cds;Parent=MsG0580025831.01.T01
Chr5 three_prime_UTR 24848770 24848993 24848770 ID=MsG0580025831.01.T01:three_prime_utr;Parent=MsG0580025831.01.T01
Gene Sequence

>MsG0580025831.01.T01

ATGGTCATGAAAATGGGTATTTCAAACCCATTCAAAGATCAATTCCACTCCATCACCAAAAAACTTTTTCCTTGGACTCTTTATGCTCTTCTTCCCATTGCTCTTCTTAGTTTATATCTCTACCCTCTTCCCTTTCTTCCTTCTACTGAATCTGAGCTTCCTCATTCAACTACTACCATTATCTCCCACTCTACACCTCCTTCTTTTACTTCTACACCCCCTCCTTCAGAGAAGGAAAATACTTTTGACACCCCCACATGTGACTACTTCAATGGAAAATGGATCCGTGATAGAAGAGGACCTTTATACAATGGAACAACATGTGGAACAATCAAAGAAGGACAAAATTGTATCAAACATGGAAGACCAGATTCTGATTATCTTTACTGGAGATGGAAACCAAGTGAATGTAAACTTCCAAGGTTTGAGCCTAACACTTTTCTTCAATTCATTGAAAACAAACATATGGCTTTTGTTGGTGATTCTATGGCTAGGAATCAGTTAGAGTCACTTCTTTGTTTGTTATCTAGTGTTTCAACACCTGATCTTGTTTACACTAGTGGTGAGGATATTAAGTTTAGAAAGTGGCATTTTGATTCTCATAATGCAAGTGTGTCTGTGTATTGGTCCCCTTTTCTTGTTCAAGGTGTTGAGAAATCAAATGACGGGCCAAATCATAATAAGTTGTATTTGGATCTTGTTGATGAAAGATGGGCAAAGGATATGGAAAAGATGGATTTTATTGTGCTTTCAATTGGTCATTGGTTTTTGCATCCTGCAGTTTACTACGAAGGTGATTCAGTTTTAGGTTGTCATTATTGTGTTGGTCTTAATCATACTGAAATTGGGTTTTATGATGTATTGAGAAAGGCTTTAAGAACTACCCTTAATAGCATAATTGATAGGAGAGGAAATAAAGGTAGTAAAATGGATGTACTAGTGACAACATTTTCACCAGCTCATTTTGAAGGTGAATGGGATAAAGCTGGTGCTTGTCCAAAGACTAAGCCTTATAGAAGTAGGGAGAAGGAACTTGAAGGAATGGATAGTGAGATGAGGAAAATTGAGGTTGAAGAAGTTGAAGATGCTAAGGTGAAAAGTAGTGAATTTGGAGGGGTTAGGTTTGAGGCACTTGATGTAACTAAATTGCCATTGTTGAGACCAGATGGTCATCCAGGTCCTTATATGTACCCTTTTCCATTTGCTAATGGGGTTCCAGAGCGTGTGCAAAATGATTGTGTTCATTGGTGTTTGCCTGGACCTATAGACACATGGAATGAGATTTTTCTGGAGATTATGAAGAAATGGGAGGACAATCCAAGAACTCAAGAGTGA
Protein sequence

>MsG0580025831.01.T01

MVMKMGISNPFKDQFHSITKKLFPWTLYALLPIALLSLYLYPLPFLPSTESELPHSTTTIISHSTPPSFTSTPPPSEKENTFDTPTCDYFNGKWIRDRRGPLYNGTTCGTIKEGQNCIKHGRPDSDYLYWRWKPSECKLPRFEPNTFLQFIENKHMAFVGDSMARNQLESLLCLLSSVSTPDLVYTSGEDIKFRKWHFDSHNASVSVYWSPFLVQGVEKSNDGPNHNKLYLDLVDERWAKDMEKMDFIVLSIGHWFLHPAVYYEGDSVLGCHYCVGLNHTEIGFYDVLRKALRTTLNSIIDRRGNKGSKMDVLVTTFSPAHFEGEWDKAGACPKTKPYRSREKELEGMDSEMRKIEVEEVEDAKVKSSEFGGVRFEALDVTKLPLLRPDGHPGPYMYPFPFANGVPERVQNDCVHWCLPGPIDTWNEIFLEIMKKWEDNPRTQE*