Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025855.01.T01 | RHN55080.1 | 79.121 | 182 | 38 | 0 | 11 | 192 | 1 | 182 | 7.94E-97 | 293 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025855.01.T01 | Q7XA40 | 42.077 | 183 | 90 | 2 | 11 | 193 | 1 | 167 | 6.14E-39 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025855.01.T01 | G7K455 | 80.645 | 186 | 32 | 1 | 11 | 192 | 1 | 186 | 3.20e-89 | 294 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380012085.01 | MsG0580025855.01 | 0.824205 | 9.158287e-54 | 8.058210e-51 |
| MsG0380012091.01 | MsG0580025855.01 | 0.814289 | 1.662355e-51 | 1.110934e-48 |
| MsG0380012319.01 | MsG0580025855.01 | 0.816528 | 5.280537e-52 | 3.750872e-49 |
| MsG0380015038.01 | MsG0580025855.01 | 0.803611 | 3.210382e-49 | 1.620488e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025855.01.T01 | MTR_5g035480 | 80.645 | 186 | 32 | 1 | 11 | 192 | 1 | 186 | 8.10e-93 | 294 |
| MsG0580025855.01.T01 | MTR_5g034770 | 79.121 | 182 | 38 | 0 | 11 | 192 | 1 | 182 | 7.15e-92 | 291 |
| MsG0580025855.01.T01 | MTR_5g035300 | 75.410 | 183 | 45 | 0 | 11 | 193 | 1 | 183 | 5.39e-91 | 278 |
| MsG0580025855.01.T01 | MTR_5g035230 | 71.585 | 183 | 48 | 1 | 11 | 193 | 1 | 179 | 2.87e-87 | 267 |
| MsG0580025855.01.T01 | MTR_5g035240 | 70.270 | 185 | 55 | 0 | 11 | 195 | 1 | 185 | 9.98e-84 | 270 |
| MsG0580025855.01.T01 | MTR_5g035280 | 72.131 | 183 | 51 | 0 | 11 | 193 | 1 | 183 | 3.08e-83 | 271 |
| MsG0580025855.01.T01 | MTR_3g026660 | 70.330 | 182 | 54 | 0 | 11 | 192 | 1 | 182 | 5.50e-82 | 266 |
| MsG0580025855.01.T01 | MTR_3g026660 | 66.667 | 45 | 15 | 0 | 206 | 250 | 138 | 182 | 4.25e-11 | 63.5 |
| MsG0580025855.01.T01 | MTR_3g026660 | 70.330 | 182 | 54 | 0 | 11 | 192 | 1 | 182 | 9.83e-82 | 266 |
| MsG0580025855.01.T01 | MTR_3g026660 | 66.667 | 45 | 15 | 0 | 206 | 250 | 138 | 182 | 4.33e-11 | 63.5 |
| MsG0580025855.01.T01 | MTR_5g037450 | 63.441 | 186 | 64 | 1 | 11 | 192 | 1 | 186 | 8.45e-71 | 235 |
| MsG0580025855.01.T01 | MTR_5g037500 | 66.484 | 182 | 61 | 0 | 11 | 192 | 1 | 182 | 1.85e-67 | 225 |
| MsG0580025855.01.T01 | MTR_4g051688 | 84.906 | 106 | 16 | 0 | 11 | 116 | 1 | 106 | 1.74e-51 | 179 |
| MsG0580025855.01.T01 | MTR_6g046130 | 40.000 | 185 | 97 | 3 | 11 | 193 | 1 | 173 | 3.37e-39 | 145 |
| MsG0580025855.01.T01 | MTR_6g052750 | 38.503 | 187 | 102 | 3 | 11 | 196 | 1 | 175 | 6.33e-39 | 135 |
| MsG0580025855.01.T01 | MTR_6g046620 | 39.459 | 185 | 98 | 3 | 11 | 193 | 1 | 173 | 7.76e-39 | 144 |
| MsG0580025855.01.T01 | MTR_5g035510 | 63.208 | 106 | 35 | 1 | 11 | 112 | 1 | 106 | 4.31e-38 | 129 |
| MsG0580025855.01.T01 | MTR_6g052760 | 39.572 | 187 | 100 | 3 | 11 | 196 | 1 | 175 | 6.93e-37 | 139 |
| MsG0580025855.01.T01 | MTR_5g037490 | 62.376 | 101 | 36 | 1 | 11 | 109 | 1 | 101 | 8.78e-37 | 126 |
| MsG0580025855.01.T01 | MTR_5g071823 | 42.391 | 184 | 89 | 3 | 11 | 192 | 1 | 169 | 5.01e-36 | 136 |
| MsG0580025855.01.T01 | MTR_5g071850 | 42.391 | 184 | 89 | 3 | 11 | 192 | 1 | 169 | 5.01e-36 | 136 |
| MsG0580025855.01.T01 | MTR_0511s0020 | 37.968 | 187 | 103 | 3 | 11 | 196 | 1 | 175 | 1.59e-35 | 134 |
| MsG0580025855.01.T01 | MTR_6g046480 | 37.433 | 187 | 103 | 3 | 11 | 195 | 1 | 175 | 1.62e-35 | 134 |
| MsG0580025855.01.T01 | MTR_8g036195 | 42.623 | 183 | 92 | 3 | 11 | 192 | 1 | 171 | 2.74e-35 | 134 |
| MsG0580025855.01.T01 | MTR_0133s0030 | 40.107 | 187 | 98 | 4 | 11 | 195 | 1 | 175 | 3.18e-35 | 134 |
| MsG0580025855.01.T01 | MTR_5g070490 | 40.761 | 184 | 92 | 3 | 11 | 192 | 1 | 169 | 3.38e-35 | 134 |
| MsG0580025855.01.T01 | MTR_5g070470 | 41.304 | 184 | 91 | 3 | 11 | 192 | 1 | 169 | 3.68e-35 | 134 |
| MsG0580025855.01.T01 | MTR_5g071220 | 40.761 | 184 | 92 | 3 | 11 | 192 | 1 | 169 | 4.23e-35 | 133 |
| MsG0580025855.01.T01 | MTR_6g046440 | 39.247 | 186 | 99 | 2 | 11 | 195 | 1 | 173 | 4.45e-35 | 133 |
| MsG0580025855.01.T01 | MTR_5g071220 | 40.761 | 184 | 92 | 3 | 11 | 192 | 1 | 169 | 5.31e-35 | 133 |
| MsG0580025855.01.T01 | MTR_5g071780 | 42.935 | 184 | 88 | 4 | 11 | 192 | 1 | 169 | 5.39e-35 | 133 |
| MsG0580025855.01.T01 | MTR_6g046930 | 38.503 | 187 | 98 | 3 | 11 | 195 | 1 | 172 | 1.19e-34 | 132 |
| MsG0580025855.01.T01 | MTR_0230s0010 | 37.968 | 187 | 103 | 3 | 11 | 196 | 1 | 175 | 1.50e-34 | 132 |
| MsG0580025855.01.T01 | MTR_5g071310 | 41.081 | 185 | 86 | 3 | 11 | 192 | 1 | 165 | 1.55e-34 | 132 |
| MsG0580025855.01.T01 | MTR_3g086070 | 37.297 | 185 | 104 | 1 | 11 | 195 | 1 | 173 | 4.55e-34 | 130 |
| MsG0580025855.01.T01 | MTR_0256s0050 | 39.247 | 186 | 99 | 2 | 11 | 195 | 1 | 173 | 8.65e-34 | 129 |
| MsG0580025855.01.T01 | MTR_0133s0020 | 39.459 | 185 | 100 | 1 | 11 | 195 | 1 | 173 | 8.74e-34 | 129 |
| MsG0580025855.01.T01 | MTR_6g047450 | 38.378 | 185 | 100 | 3 | 11 | 193 | 1 | 173 | 1.07e-33 | 129 |
| MsG0580025855.01.T01 | MTR_3g023320 | 39.785 | 186 | 99 | 3 | 11 | 195 | 1 | 174 | 1.37e-33 | 129 |
| MsG0580025855.01.T01 | MTR_1g023600 | 41.304 | 184 | 88 | 2 | 11 | 192 | 1 | 166 | 1.66e-33 | 129 |
| MsG0580025855.01.T01 | MTR_6g046550 | 36.269 | 193 | 103 | 4 | 11 | 195 | 1 | 181 | 2.04e-33 | 128 |
| MsG0580025855.01.T01 | MTR_0144s0060 | 36.757 | 185 | 103 | 2 | 11 | 193 | 1 | 173 | 3.81e-33 | 127 |
| MsG0580025855.01.T01 | MTR_1g022380 | 37.500 | 184 | 93 | 2 | 11 | 192 | 1 | 164 | 1.22e-32 | 126 |
| MsG0580025855.01.T01 | MTR_5g071570 | 40.426 | 188 | 94 | 3 | 11 | 195 | 1 | 173 | 2.05e-32 | 125 |
| MsG0580025855.01.T01 | MTR_5g072340 | 40.761 | 184 | 92 | 3 | 11 | 192 | 1 | 169 | 5.19e-31 | 121 |
| MsG0580025855.01.T01 | MTR_5g072140 | 39.572 | 187 | 96 | 3 | 11 | 195 | 1 | 172 | 3.70e-30 | 119 |
| MsG0580025855.01.T01 | MTR_5g070960 | 39.130 | 184 | 95 | 3 | 11 | 192 | 1 | 169 | 6.32e-30 | 118 |
| MsG0580025855.01.T01 | MTR_5g071470 | 39.130 | 184 | 95 | 3 | 11 | 192 | 1 | 169 | 4.75e-29 | 116 |
| MsG0580025855.01.T01 | MTR_6g046570 | 38.824 | 170 | 90 | 2 | 27 | 196 | 17 | 172 | 2.62e-28 | 114 |
| MsG0580025855.01.T01 | MTR_5g039570 | 36.264 | 182 | 78 | 3 | 11 | 192 | 1 | 144 | 3.84e-27 | 110 |
| MsG0580025855.01.T01 | MTR_5g071800 | 37.433 | 187 | 100 | 3 | 11 | 195 | 1 | 172 | 2.42e-26 | 108 |
| MsG0580025855.01.T01 | MTR_4g055630 | 36.364 | 187 | 102 | 3 | 11 | 195 | 1 | 172 | 4.48e-26 | 107 |
| MsG0580025855.01.T01 | MTR_4g126000 | 41.259 | 143 | 69 | 2 | 11 | 153 | 1 | 128 | 1.45e-25 | 105 |
| MsG0580025855.01.T01 | MTR_5g072250 | 36.364 | 187 | 102 | 3 | 11 | 195 | 1 | 172 | 8.66e-25 | 103 |
| MsG0580025855.01.T01 | MTR_6g452880 | 38.235 | 136 | 72 | 2 | 11 | 146 | 1 | 124 | 1.35e-24 | 95.5 |
| MsG0580025855.01.T01 | MTR_5g071670 | 36.413 | 184 | 95 | 2 | 11 | 192 | 1 | 164 | 2.17e-24 | 102 |
| MsG0580025855.01.T01 | MTR_7g071940 | 34.759 | 187 | 100 | 2 | 11 | 195 | 1 | 167 | 6.60e-24 | 101 |
| MsG0580025855.01.T01 | MTR_6g453400 | 38.462 | 130 | 68 | 2 | 11 | 140 | 1 | 118 | 1.86e-21 | 87.0 |
| MsG0580025855.01.T01 | MTR_8g011180 | 32.515 | 163 | 106 | 1 | 29 | 191 | 23 | 181 | 5.67e-20 | 89.7 |
| MsG0580025855.01.T01 | MTR_5g040900 | 36.552 | 145 | 78 | 2 | 11 | 155 | 1 | 131 | 1.62e-19 | 88.2 |
| MsG0580025855.01.T01 | MTR_5g071450 | 34.595 | 185 | 89 | 7 | 41 | 213 | 10 | 174 | 1.76e-19 | 86.7 |
| MsG0580025855.01.T01 | MTR_2g078070 | 27.684 | 177 | 123 | 2 | 30 | 203 | 24 | 198 | 3.84e-19 | 87.4 |
| MsG0580025855.01.T01 | MTR_1g078490 | 31.016 | 187 | 120 | 4 | 11 | 193 | 1 | 182 | 1.82e-16 | 79.3 |
| MsG0580025855.01.T01 | MTR_5g035530 | 55.224 | 67 | 30 | 0 | 132 | 198 | 1 | 67 | 4.75e-16 | 78.2 |
| MsG0580025855.01.T01 | MTR_8g011170 | 27.604 | 192 | 113 | 5 | 1 | 191 | 1 | 167 | 9.19e-16 | 77.4 |
| MsG0580025855.01.T01 | MTR_6g046450 | 33.898 | 118 | 65 | 3 | 74 | 190 | 144 | 249 | 3.52e-15 | 75.5 |
| MsG0580025855.01.T01 | MTR_4g050790 | 27.624 | 181 | 126 | 2 | 13 | 193 | 2 | 177 | 8.04e-15 | 74.3 |
| MsG0580025855.01.T01 | MTR_3g015500 | 27.624 | 181 | 126 | 2 | 13 | 193 | 40 | 215 | 1.32e-14 | 73.9 |
| MsG0580025855.01.T01 | MTR_0005s0200 | 34.965 | 143 | 86 | 3 | 50 | 192 | 68 | 203 | 4.35e-14 | 72.4 |
| MsG0580025855.01.T01 | MTR_4g094540 | 29.787 | 188 | 125 | 3 | 11 | 193 | 1 | 186 | 4.56e-14 | 72.0 |
| MsG0580025855.01.T01 | MTR_4g119195 | 28.477 | 151 | 99 | 3 | 1 | 151 | 1 | 142 | 1.73e-13 | 70.5 |
| MsG0580025855.01.T01 | MTR_4g119195 | 28.477 | 151 | 99 | 3 | 1 | 151 | 1 | 142 | 2.20e-13 | 70.1 |
| MsG0580025855.01.T01 | MTR_5g037460 | 57.377 | 61 | 25 | 1 | 132 | 192 | 1 | 60 | 2.48e-13 | 70.1 |
| MsG0580025855.01.T01 | MTR_8g445980 | 27.586 | 203 | 119 | 4 | 1 | 195 | 1 | 183 | 3.46e-13 | 69.7 |
| MsG0580025855.01.T01 | MTR_7g013560 | 26.733 | 202 | 119 | 5 | 1 | 192 | 1 | 183 | 3.85e-13 | 69.3 |
| MsG0580025855.01.T01 | MTR_8g011550 | 28.358 | 201 | 115 | 7 | 1 | 191 | 1 | 182 | 1.13e-12 | 68.2 |
| MsG0580025855.01.T01 | MTR_3g015550 | 28.108 | 185 | 128 | 2 | 9 | 193 | 35 | 214 | 1.16e-12 | 68.2 |
| MsG0580025855.01.T01 | MTR_5g076840 | 26.733 | 202 | 125 | 7 | 1 | 195 | 1 | 186 | 1.37e-12 | 67.8 |
| MsG0580025855.01.T01 | MTR_3g015550 | 28.729 | 181 | 124 | 2 | 13 | 193 | 2 | 177 | 3.20e-12 | 66.6 |
| MsG0580025855.01.T01 | MTR_0065s0100 | 25.521 | 192 | 132 | 5 | 1 | 191 | 1 | 182 | 3.57e-12 | 66.6 |
| MsG0580025855.01.T01 | MTR_8g027540 | 27.174 | 184 | 109 | 5 | 19 | 191 | 13 | 182 | 5.77e-12 | 65.9 |
| MsG0580025855.01.T01 | MTR_3g020890 | 34.826 | 201 | 98 | 8 | 1 | 193 | 1 | 176 | 7.23e-12 | 65.9 |
| MsG0580025855.01.T01 | MTR_8g010440 | 28.177 | 181 | 105 | 6 | 23 | 191 | 14 | 181 | 1.47e-11 | 64.7 |
| MsG0580025855.01.T01 | MTR_7g091550 | 27.660 | 188 | 126 | 3 | 13 | 193 | 2 | 186 | 1.52e-11 | 64.7 |
| MsG0580025855.01.T01 | MTR_8g037220 | 25.381 | 197 | 134 | 5 | 1 | 191 | 770 | 959 | 1.84e-11 | 64.7 |
| MsG0580025855.01.T01 | MTR_3g014470 | 31.132 | 212 | 108 | 10 | 11 | 200 | 1 | 196 | 1.98e-11 | 64.3 |
| MsG0580025855.01.T01 | MTR_3g014320 | 29.762 | 168 | 94 | 7 | 44 | 200 | 43 | 197 | 2.12e-11 | 64.3 |
| MsG0580025855.01.T01 | MTR_8g037220 | 25.381 | 197 | 134 | 5 | 1 | 191 | 991 | 1180 | 2.23e-11 | 64.3 |
| MsG0580025855.01.T01 | MTR_6g037690 | 27.815 | 151 | 100 | 4 | 1 | 151 | 1 | 142 | 3.33e-11 | 62.8 |
| MsG0580025855.01.T01 | MTR_3g014080 | 28.758 | 153 | 99 | 4 | 52 | 200 | 50 | 196 | 4.25e-11 | 63.5 |
| MsG0580025855.01.T01 | MTR_3g014080 | 28.758 | 153 | 99 | 4 | 52 | 200 | 50 | 196 | 4.59e-11 | 63.2 |
| MsG0580025855.01.T01 | MTR_7g091140 | 28.249 | 177 | 104 | 5 | 25 | 192 | 19 | 181 | 8.58e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGACTCTGTTGTAAAATT+AGG | 0.199100 | 5:+25194911 | MsG0580025855.01.T01:CDS |
| GTTGACTATGGTGTAAAATT+AGG | 0.217033 | 5:+25194737 | MsG0580025855.01.T01:CDS |
| TAACGGAAGGCAACTTGTTT+TGG | 0.249605 | 5:-25194518 | None:intergenic |
| AAGGAGCTTAGCCTATTTCT+TGG | 0.336553 | 5:+25194248 | MsG0580025855.01.T01:CDS |
| ATTTCTCAACCTCAAGTCTA+TGG | 0.346516 | 5:+25194677 | MsG0580025855.01.T01:CDS |
| GTACTCCAACTCCAGTGCTT+TGG | 0.380754 | 5:-25194435 | None:intergenic |
| AGAAAAGCGGGTTCCTTGAC+TGG | 0.382931 | 5:+25194636 | MsG0580025855.01.T01:CDS |
| ACTTGAGGTTGAGAAATGAT+TGG | 0.426723 | 5:-25194671 | None:intergenic |
| CTTGAGGTTGAGAAATGATT+GGG | 0.428863 | 5:-25194670 | None:intergenic |
| CTCCAACTCCAGTGCTTTGG+TGG | 0.430845 | 5:-25194432 | None:intergenic |
| AAGCTTGATCACTACAATCA+AGG | 0.480844 | 5:+25194298 | MsG0580025855.01.T01:CDS |
| CTTATCAGTTAGTCCACCCT+TGG | 0.495451 | 5:-25194465 | None:intergenic |
| ATTCACTGACAAAGCTATCA+AGG | 0.497822 | 5:+25194349 | MsG0580025855.01.T01:CDS |
| CAACCTCAAGTCTATGGAAG+AGG | 0.511968 | 5:+25194683 | MsG0580025855.01.T01:CDS |
| TTACCTCTTCCATCGACTTG+AGG | 0.520462 | 5:-25194860 | None:intergenic |
| CGAGTGTGCCACCAAAGCAC+TGG | 0.524126 | 5:+25194424 | MsG0580025855.01.T01:CDS |
| CTGTTAGAGAGGAGAAAAGC+GGG | 0.525910 | 5:+25194624 | MsG0580025855.01.T01:CDS |
| CTGTTAGAGAGGAGAAAAGC+GGG | 0.525910 | 5:+25194798 | MsG0580025855.01.T01:CDS |
| CTGACAAAGCTATCAAGGAT+TGG | 0.529378 | 5:+25194354 | MsG0580025855.01.T01:CDS |
| TTACCTCTTCCATAGACTTG+AGG | 0.529712 | 5:-25194686 | None:intergenic |
| CAAGGGTGGACTAACTGATA+AGG | 0.529900 | 5:+25194466 | MsG0580025855.01.T01:CDS |
| CAACCTCAAGTCGATGGAAG+AGG | 0.532301 | 5:+25194857 | MsG0580025855.01.T01:CDS |
| TTTCTCCTCTCTAACAGTCT+CGG | 0.532614 | 5:-25194618 | None:intergenic |
| TTTCTCCTCTCTAACAGTCT+CGG | 0.532614 | 5:-25194792 | None:intergenic |
| TGCCACCAAAGCACTGGAGT+TGG | 0.533384 | 5:+25194430 | MsG0580025855.01.T01:CDS |
| AAATGATTGAGGTGGTTTGA+AGG | 0.537854 | 5:-25194832 | None:intergenic |
| AGTCTCGGTCAAATGAAACT+TGG | 0.544853 | 5:-25194603 | None:intergenic |
| TCACGTCCTTGACGACATCT+TGG | 0.546508 | 5:+25194400 | MsG0580025855.01.T01:CDS |
| AGGTGGTTTGAAGGCAGTCA+AGG | 0.557320 | 5:-25194823 | None:intergenic |
| ATTTCTCAACCTCAAGTCGA+TGG | 0.559233 | 5:+25194851 | MsG0580025855.01.T01:CDS |
| TTTCAACTCACTCGTTCAGA+AGG | 0.560645 | 5:+25194229 | MsG0580025855.01.T01:CDS |
| GTTGGAGTACAAAAGATCCA+AGG | 0.589275 | 5:+25194448 | MsG0580025855.01.T01:CDS |
| GGCTACTCTTCAAGATGCTG+AGG | 0.592308 | 5:+25194319 | MsG0580025855.01.T01:CDS |
| GGGTGGTTTGAAGCCAGTCA+AGG | 0.596453 | 5:-25194649 | None:intergenic |
| TTTGACCGAGACTGTTAGAG+AGG | 0.601334 | 5:+25194613 | MsG0580025855.01.T01:CDS |
| TTTGACCGAGACTGTTAGAG+AGG | 0.601334 | 5:+25194787 | MsG0580025855.01.T01:CDS |
| TTGAGGTTGAGAAATGATTG+AGG | 0.627903 | 5:-25194843 | None:intergenic |
| CGAGTGAGTTGAAATTGTCA+CGG | 0.629471 | 5:-25194220 | None:intergenic |
| TTGGAGTACAAAAGATCCAA+GGG | 0.640265 | 5:+25194449 | MsG0580025855.01.T01:CDS |
| ACTGTTAGAGAGGAGAAAAG+CGG | 0.653455 | 5:+25194623 | MsG0580025855.01.T01:CDS |
| ACTGTTAGAGAGGAGAAAAG+CGG | 0.653455 | 5:+25194797 | MsG0580025855.01.T01:CDS |
| ACTCGTCCAAGATGTCGTCA+AGG | 0.672402 | 5:-25194406 | None:intergenic |
| AGGTTGAGAAATGATTGGGG+TGG | 0.678128 | 5:-25194666 | None:intergenic |
| TTGAGGTTGAGAAATGATTG+GGG | 0.692759 | 5:-25194669 | None:intergenic |
| AGGTTGAGAAATGATTGAGG+TGG | 0.717041 | 5:-25194840 | None:intergenic |
| GAGTACAAAAGATCCAAGGG+TGG | 0.728876 | 5:+25194452 | MsG0580025855.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAACAAACTTTAGATTTTT+TGG | + | Chr5:25194889-25194908 | MsG0580025855.01.T01:CDS | 20.0% |
| !!! | GAAACAAATTGCAAATTTTT+TGG | + | Chr5:25194715-25194734 | MsG0580025855.01.T01:CDS | 20.0% |
| !!! | TGTTTTCTTCAAAAAAAAGA+TGG | + | Chr5:25194175-25194194 | MsG0580025855.01.T01:CDS | 20.0% |
| !!! | TTTTCTTAGCAATTTTGTAA+CGG | - | Chr5:25194538-25194557 | None:intergenic | 20.0% |
| GTTGACTATGGTGTAAAATT+AGG | + | Chr5:25194737-25194756 | MsG0580025855.01.T01:CDS | 30.0% | |
| ! | GTTGACTCTGTTGTAAAATT+AGG | + | Chr5:25194911-25194930 | MsG0580025855.01.T01:CDS | 30.0% |
| !!! | CTTTTTCGAAACCAAGAAAT+AGG | - | Chr5:25194262-25194281 | None:intergenic | 30.0% |
| !!! | GCAAATTTTTTGGTTGACTA+TGG | + | Chr5:25194725-25194744 | MsG0580025855.01.T01:CDS | 30.0% |
| AAATGATTGAGGTGGTTTGA+AGG | - | Chr5:25194835-25194854 | None:intergenic | 35.0% | |
| AAGCTTGATCACTACAATCA+AGG | + | Chr5:25194298-25194317 | MsG0580025855.01.T01:CDS | 35.0% | |
| ACTTGAGGTTGAGAAATGAT+TGG | - | Chr5:25194674-25194693 | None:intergenic | 35.0% | |
| ATTCACTGACAAAGCTATCA+AGG | + | Chr5:25194349-25194368 | MsG0580025855.01.T01:CDS | 35.0% | |
| ATTTCTCAACCTCAAGTCTA+TGG | + | Chr5:25194677-25194696 | MsG0580025855.01.T01:CDS | 35.0% | |
| CTTGAGGTTGAGAAATGATT+GGG | - | Chr5:25194673-25194692 | None:intergenic | 35.0% | |
| TTGAGGTTGAGAAATGATTG+AGG | - | Chr5:25194672-25194691 | None:intergenic | 35.0% | |
| TTGAGGTTGAGAAATGATTG+GGG | - | Chr5:25194672-25194691 | None:intergenic | 35.0% | |
| TTGGAGTACAAAAGATCCAA+GGG | + | Chr5:25194449-25194468 | MsG0580025855.01.T01:CDS | 35.0% | |
| !! | CTTAGCAATTTTGTAACGGA+AGG | - | Chr5:25194534-25194553 | None:intergenic | 35.0% |
| !!! | AACTTGTTTTGGATGAAGAG+AGG | - | Chr5:25194510-25194529 | None:intergenic | 35.0% |
| ACTGTTAGAGAGGAGAAAAG+CGG | + | Chr5:25194623-25194642 | MsG0580025855.01.T01:CDS | 40.0% | |
| AGGTTGAGAAATGATTGAGG+TGG | - | Chr5:25194843-25194862 | None:intergenic | 40.0% | |
| AGTCTCGGTCAAATGAAACT+TGG | - | Chr5:25194606-25194625 | None:intergenic | 40.0% | |
| ATTTCTCAACCTCAAGTCGA+TGG | + | Chr5:25194851-25194870 | MsG0580025855.01.T01:CDS | 40.0% | |
| CTGACAAAGCTATCAAGGAT+TGG | + | Chr5:25194354-25194373 | MsG0580025855.01.T01:CDS | 40.0% | |
| GTTGGAGTACAAAAGATCCA+AGG | + | Chr5:25194448-25194467 | MsG0580025855.01.T01:CDS | 40.0% | |
| TTACCTCTTCCATAGACTTG+AGG | - | Chr5:25194689-25194708 | None:intergenic | 40.0% | |
| TTTCAACTCACTCGTTCAGA+AGG | + | Chr5:25194229-25194248 | MsG0580025855.01.T01:CDS | 40.0% | |
| TTTCTCCTCTCTAACAGTCT+CGG | - | Chr5:25194621-25194640 | None:intergenic | 40.0% | |
| ! | AAGGAGCTTAGCCTATTTCT+TGG | + | Chr5:25194248-25194267 | MsG0580025855.01.T01:CDS | 40.0% |
| ! | CGAGTGAGTTGAAATTGTCA+CGG | - | Chr5:25194223-25194242 | None:intergenic | 40.0% |
| !! | TAACGGAAGGCAACTTGTTT+TGG | - | Chr5:25194521-25194540 | None:intergenic | 40.0% |
| AGGTTGAGAAATGATTGGGG+TGG | - | Chr5:25194669-25194688 | None:intergenic | 45.0% | |
| CAACCTCAAGTCTATGGAAG+AGG | + | Chr5:25194683-25194702 | MsG0580025855.01.T01:CDS | 45.0% | |
| CTGTTAGAGAGGAGAAAAGC+GGG | + | Chr5:25194624-25194643 | MsG0580025855.01.T01:CDS | 45.0% | |
| CTTATCAGTTAGTCCACCCT+TGG | - | Chr5:25194468-25194487 | None:intergenic | 45.0% | |
| GAGTACAAAAGATCCAAGGG+TGG | + | Chr5:25194452-25194471 | MsG0580025855.01.T01:CDS | 45.0% | |
| TTTGACCGAGACTGTTAGAG+AGG | + | Chr5:25194613-25194632 | MsG0580025855.01.T01:CDS | 45.0% | |
| !! | CAAGGGTGGACTAACTGATA+AGG | + | Chr5:25194466-25194485 | MsG0580025855.01.T01:CDS | 45.0% |
| !! | TTACCTCTTCCATCGACTTG+AGG | - | Chr5:25194863-25194882 | None:intergenic | 45.0% |
| ACTCGTCCAAGATGTCGTCA+AGG | - | Chr5:25194409-25194428 | None:intergenic | 50.0% | |
| AGGTGGTTTGAAGGCAGTCA+AGG | - | Chr5:25194826-25194845 | None:intergenic | 50.0% | |
| CAACCTCAAGTCGATGGAAG+AGG | + | Chr5:25194857-25194876 | MsG0580025855.01.T01:CDS | 50.0% | |
| GGCTACTCTTCAAGATGCTG+AGG | + | Chr5:25194319-25194338 | MsG0580025855.01.T01:CDS | 50.0% | |
| GTACTCCAACTCCAGTGCTT+TGG | - | Chr5:25194438-25194457 | None:intergenic | 50.0% | |
| TCACGTCCTTGACGACATCT+TGG | + | Chr5:25194400-25194419 | MsG0580025855.01.T01:CDS | 50.0% | |
| ! | AGAAAAGCGGGTTCCTTGAC+TGG | + | Chr5:25194636-25194655 | MsG0580025855.01.T01:CDS | 50.0% |
| CTCCAACTCCAGTGCTTTGG+TGG | - | Chr5:25194435-25194454 | None:intergenic | 55.0% | |
| ! | GGGTGGTTTGAAGCCAGTCA+AGG | - | Chr5:25194652-25194671 | None:intergenic | 55.0% |
| !! | TGCCACCAAAGCACTGGAGT+TGG | + | Chr5:25194430-25194449 | MsG0580025855.01.T01:CDS | 55.0% |
| CGAGTGTGCCACCAAAGCAC+TGG | + | Chr5:25194424-25194443 | MsG0580025855.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 25194164 | 25194955 | 25194164 | ID=MsG0580025855.01;Name=MsG0580025855.01 |
| Chr5 | mRNA | 25194164 | 25194955 | 25194164 | ID=MsG0580025855.01.T01;Parent=MsG0580025855.01;Name=MsG0580025855.01.T01;_AED=0.27;_eAED=0.27;_QI=0|-1|0|1|-1|1|1|0|263 |
| Chr5 | exon | 25194164 | 25194955 | 25194164 | ID=MsG0580025855.01.T01:exon:4050;Parent=MsG0580025855.01.T01 |
| Chr5 | CDS | 25194164 | 25194955 | 25194164 | ID=MsG0580025855.01.T01:cds;Parent=MsG0580025855.01.T01 |
| Gene Sequence |
| Protein sequence |
