AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025957.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025957.01.T01 MTR_8g098925 50.806 124 18 2 1 95 53 162 2.09e-32 111
MsG0580025957.01.T01 MTR_5g022720 69.231 65 20 0 45 109 1 65 2.23e-24 94.0
MsG0580025957.01.T01 MTR_2g088140 81.250 48 9 0 62 109 52 99 7.12e-22 84.0
MsG0580025957.01.T01 MTR_1g103580 43.269 104 36 2 5 108 138 218 1.30e-21 86.7
MsG0580025957.01.T01 MTR_5g037920 37.500 136 56 2 2 108 73 208 8.76e-20 81.3
MsG0580025957.01.T01 MTR_8g022750 38.095 105 53 1 4 96 182 286 4.29e-18 77.4
MsG0580025957.01.T01 MTR_4g029360 34.646 127 54 1 1 98 101 227 2.82e-17 74.3
MsG0580025957.01.T01 MTR_7g006380 36.283 113 58 2 4 108 43 149 5.58e-17 72.8
MsG0580025957.01.T01 MTR_2g047100 41.000 100 45 2 12 97 3 102 1.76e-16 69.3
MsG0580025957.01.T01 MTR_8g028785 35.849 106 63 1 5 105 260 365 2.02e-15 70.9
MsG0580025957.01.T01 MTR_0093s0070 48.101 79 12 1 31 80 127 205 4.17e-15 68.2
MsG0580025957.01.T01 MTR_3g010950 37.963 108 62 3 3 108 2 106 7.04e-15 66.2
MsG0580025957.01.T01 MTR_6g045427 39.535 86 47 2 4 86 150 233 5.13e-14 65.9
MsG0580025957.01.T01 MTR_4g128080 50.000 60 30 0 49 108 5 64 8.29e-14 61.6
MsG0580025957.01.T01 MTR_5g075610 41.250 80 43 2 25 102 20 97 1.57e-12 59.3
MsG0580025957.01.T01 MTR_4g064590 38.462 78 44 1 31 108 61 134 1.70e-12 60.5
MsG0580025957.01.T01 MTR_8g031080 33.929 112 61 3 5 108 267 373 1.52e-11 60.1
MsG0580025957.01.T01 MTR_1g081930 30.435 115 51 2 4 89 15 129 5.72e-11 55.8
MsG0580025957.01.T03 MTR_8g098925 72.581 124 20 1 1 124 53 162 1.00e-59 182
MsG0580025957.01.T03 MTR_6g089560 50.435 115 57 0 1 115 90 204 5.41e-39 132
MsG0580025957.01.T03 MTR_0093s0070 75.949 79 19 0 31 109 127 205 2.94e-38 129
MsG0580025957.01.T03 MTR_5g037920 49.635 137 67 2 2 137 73 208 4.63e-37 127
MsG0580025957.01.T03 MTR_1g017840 54.808 104 47 0 4 107 55 158 4.69e-34 117
MsG0580025957.01.T03 MTR_1g088650 48.819 127 65 0 4 130 63 189 8.19e-34 117
MsG0580025957.01.T03 MTR_4g029360 46.457 127 68 0 1 127 101 227 1.98e-32 114
MsG0580025957.01.T03 MTR_8g099580 49.057 106 54 0 5 110 184 289 7.23e-30 110
MsG0580025957.01.T03 MTR_5g075610 50.505 99 47 1 33 131 1 97 7.85e-29 102
MsG0580025957.01.T03 MTR_7g013520 47.107 121 63 1 6 125 49 169 3.31e-28 102
MsG0580025957.01.T03 MTR_6g016265 44.444 108 60 0 6 113 18 125 4.76e-27 98.2
MsG0580025957.01.T03 MTR_2g027020 38.519 135 79 1 5 139 6 136 4.83e-27 100
MsG0580025957.01.T03 MTR_6g453270 52.439 82 39 0 5 86 278 359 1.54e-26 102
MsG0580025957.01.T03 MTR_5g083200 39.167 120 73 0 4 123 75 194 8.27e-26 97.1
MsG0580025957.01.T03 MTR_0002s1220 39.167 120 73 0 4 123 75 194 8.27e-26 97.1
MsG0580025957.01.T03 MTR_3g116210 37.288 118 74 0 1 118 71 188 1.99e-25 95.9
MsG0580025957.01.T03 MTR_5g036320 53.086 81 38 0 6 86 58 138 5.31e-25 93.6
MsG0580025957.01.T03 MTR_3g111390 38.333 120 74 0 4 123 75 194 5.78e-25 94.7
MsG0580025957.01.T03 MTR_1g070820 38.333 120 74 0 4 123 75 194 5.78e-25 94.7
MsG0580025957.01.T03 MTR_5g049680 53.086 81 38 0 6 86 10 90 9.23e-25 91.7
MsG0580025957.01.T03 MTR_1g069475 41.346 104 61 0 5 108 142 245 1.83e-24 94.7
MsG0580025957.01.T03 MTR_6g046670 61.039 77 30 0 4 80 100 176 2.69e-24 93.2
MsG0580025957.01.T03 MTR_7g063280 38.462 104 64 0 27 130 72 175 4.02e-24 92.0
MsG0580025957.01.T03 MTR_5g022720 69.231 65 20 0 74 138 1 65 5.46e-24 94.0
MsG0580025957.01.T03 MTR_8g036780 50.617 81 40 0 5 85 12 92 6.22e-24 89.7
MsG0580025957.01.T03 MTR_5g085210 37.879 132 66 1 6 137 98 213 1.27e-23 92.4
MsG0580025957.01.T03 MTR_1g081930 39.655 116 70 0 3 118 14 129 1.37e-23 89.4
MsG0580025957.01.T03 MTR_5g034450 53.947 76 35 0 11 86 33 108 1.63e-23 89.0
MsG0580025957.01.T03 MTR_2g017550 53.333 75 35 0 5 79 86 160 1.81e-23 90.1
MsG0580025957.01.T03 MTR_8g031800 53.846 78 36 0 9 86 13 90 3.47e-23 87.8
MsG0580025957.01.T03 MTR_2g047100 43.478 115 50 1 12 126 3 102 5.15e-23 87.0
MsG0580025957.01.T03 MTR_4g094272 56.338 71 30 1 4 74 71 140 8.92e-23 87.8
MsG0580025957.01.T03 MTR_1g045340 38.462 117 72 0 7 123 64 180 1.09e-22 88.6
MsG0580025957.01.T03 MTR_1g034430 39.815 108 65 0 6 113 74 181 1.83e-22 88.2
MsG0580025957.01.T03 MTR_8g031080 41.353 133 52 4 5 137 267 373 2.00e-22 91.7
MsG0580025957.01.T03 MTR_1g074090 47.619 84 44 0 3 86 57 140 6.14e-22 85.9
MsG0580025957.01.T03 MTR_2g088140 81.250 48 9 0 91 138 52 99 9.51e-22 84.7
MsG0580025957.01.T03 MTR_0015s0120 57.333 75 32 0 12 86 278 352 1.17e-21 89.4
MsG0580025957.01.T03 MTR_2g059470 47.500 80 42 0 31 110 4 83 1.48e-21 82.8
MsG0580025957.01.T03 MTR_7g006380 35.821 134 59 1 4 137 43 149 1.57e-21 85.5
MsG0580025957.01.T03 MTR_8g022750 37.398 123 60 1 4 126 182 287 2.32e-21 87.4
MsG0580025957.01.T03 MTR_1g054905 59.420 69 28 0 2 70 76 144 3.42e-21 84.3
MsG0580025957.01.T03 MTR_7g078610 56.061 66 29 0 4 69 28 93 1.59e-20 80.5
MsG0580025957.01.T03 MTR_6g034915 33.333 156 57 2 12 137 10 148 2.15e-20 82.4
MsG0580025957.01.T03 MTR_8g079540 42.222 90 52 0 4 93 25 114 2.31e-20 80.9
MsG0580025957.01.T03 MTR_1g022215 50.000 68 34 0 14 81 1 68 2.44e-20 80.1
MsG0580025957.01.T03 MTR_1g050488 48.000 75 39 0 12 86 83 157 3.65e-20 81.6
MsG0580025957.01.T03 MTR_4g064590 45.161 93 45 2 47 137 46 134 1.04e-19 80.5
MsG0580025957.01.T03 MTR_8g028785 36.923 130 58 1 5 134 260 365 2.19e-19 83.2
MsG0580025957.01.T03 MTR_8g059135 38.095 105 64 1 17 121 19 122 4.45e-19 77.8
MsG0580025957.01.T03 MTR_6g033225 47.368 76 40 0 11 86 54 129 9.80e-19 77.4
MsG0580025957.01.T03 MTR_4g008270 46.914 81 43 0 28 108 11 91 1.20e-18 75.9
MsG0580025957.01.T03 MTR_1g099430 46.429 84 43 1 2 83 100 183 1.28e-18 78.6
MsG0580025957.01.T03 MTR_8g038340 44.444 81 45 0 4 84 166 246 1.95e-18 79.0
MsG0580025957.01.T03 MTR_6g016355 44.156 77 43 0 14 90 103 179 1.07e-17 75.5
MsG0580025957.01.T03 MTR_1g018300 44.954 109 54 2 6 114 42 144 5.32e-17 75.1
MsG0580025957.01.T03 MTR_3g462490 30.986 142 47 1 2 143 76 166 8.02e-17 76.3
MsG0580025957.01.T03 MTR_1g074240 49.231 65 33 0 31 95 4 68 8.08e-17 70.5
MsG0580025957.01.T03 MTR_1g109000 41.975 81 47 0 6 86 5 85 1.27e-16 70.5
MsG0580025957.01.T03 MTR_6g045427 36.036 111 43 1 4 114 150 232 4.26e-16 72.4
MsG0580025957.01.T03 MTR_2g055490 40.566 106 30 1 32 137 12 84 6.65e-16 69.3
MsG0580025957.01.T03 MTR_5g042170 70.455 44 13 0 6 49 29 72 9.00e-16 68.9
MsG0580025957.01.T03 MTR_4g046027 53.968 63 29 0 7 69 107 169 3.39e-15 68.9
MsG0580025957.01.T03 MTR_7g053400 34.545 110 38 1 28 137 176 251 7.11e-15 69.3
MsG0580025957.01.T03 MTR_8g069135 55.738 61 22 1 4 59 166 226 8.70e-14 67.0
MsG0580025957.01.T03 MTR_4g128080 50.000 60 30 0 78 137 5 64 1.08e-13 62.4
MsG0580025957.01.T03 MTR_1g045560 37.143 105 50 2 33 137 1 89 2.52e-13 62.0
MsG0580025957.01.T03 MTR_6g023630 58.333 48 19 1 42 88 4 51 3.15e-13 61.6
MsG0580025957.01.T03 MTR_3g064060 55.556 45 20 0 3 47 13 57 4.77e-13 60.5
MsG0580025957.01.T03 MTR_5g068115 58.140 43 18 0 5 47 12 54 8.12e-13 60.1
MsG0580025957.01.T03 MTR_8g045330 43.939 66 37 0 4 69 74 139 1.24e-12 62.0
MsG0580025957.01.T03 MTR_8g088220 40.541 74 44 0 37 110 4 77 3.14e-12 58.9
MsG0580025957.01.T03 MTR_8g036690 34.884 86 40 1 58 143 2 71 3.88e-12 58.5
MsG0580025957.01.T03 MTR_1g087480 50.909 55 27 0 5 59 166 220 2.55e-11 59.3
MsG0580025957.01.T03 MTR_1964s0010 50.000 46 23 0 5 50 47 92 5.92e-11 56.2
MsG0580025957.01.T03 MTR_3g026270 44.231 52 29 0 35 86 3 54 7.06e-11 55.1
MsG0580025957.01.T03 MTR_4g035890 42.105 57 33 0 5 61 92 148 7.56e-11 57.0
MsG0580025957.01.T02 MTR_8g098925 72.973 148 26 1 1 148 29 162 4.49e-72 214
MsG0580025957.01.T02 MTR_6g089560 49.180 122 62 0 18 139 83 204 3.35e-41 138
MsG0580025957.01.T02 MTR_0093s0070 52.632 133 22 1 1 133 114 205 3.35e-38 130
MsG0580025957.01.T02 MTR_5g037920 47.651 149 76 2 14 161 61 208 7.32e-38 129
MsG0580025957.01.T02 MTR_1g017840 50.893 112 55 0 20 131 47 158 3.93e-34 118
MsG0580025957.01.T02 MTR_1g088650 47.368 133 70 0 22 154 57 189 5.09e-34 119
MsG0580025957.01.T02 MTR_4g029360 44.056 143 80 0 9 151 85 227 1.73e-33 118
MsG0580025957.01.T02 MTR_8g099580 50.000 104 52 0 29 132 184 287 1.19e-29 110
MsG0580025957.01.T02 MTR_5g075610 50.505 99 47 1 57 155 1 97 9.56e-29 102
MsG0580025957.01.T02 MTR_7g013520 44.361 133 73 1 18 149 37 169 3.17e-28 103
MsG0580025957.01.T02 MTR_6g453270 45.192 104 55 1 9 110 256 359 4.64e-28 107
MsG0580025957.01.T02 MTR_2g027020 39.394 132 76 1 29 160 6 133 3.87e-27 101
MsG0580025957.01.T02 MTR_6g016265 40.984 122 72 0 16 137 4 125 5.20e-27 99.0
MsG0580025957.01.T02 MTR_3g116210 37.190 121 76 0 22 142 68 188 5.23e-26 98.2
MsG0580025957.01.T02 MTR_5g083200 35.821 134 86 0 14 147 61 194 7.92e-26 97.8
MsG0580025957.01.T02 MTR_0002s1220 35.821 134 86 0 14 147 61 194 7.92e-26 97.8
MsG0580025957.01.T02 MTR_3g111390 35.075 134 87 0 14 147 61 194 4.34e-25 95.9
MsG0580025957.01.T02 MTR_1g070820 35.075 134 87 0 14 147 61 194 4.34e-25 95.9
MsG0580025957.01.T02 MTR_5g036320 48.485 99 48 1 12 110 43 138 4.68e-25 94.4
MsG0580025957.01.T02 MTR_1g069475 39.286 112 68 0 21 132 134 245 9.95e-25 96.3
MsG0580025957.01.T02 MTR_6g046670 53.846 91 42 0 14 104 86 176 1.02e-24 95.1
MsG0580025957.01.T02 MTR_5g049680 53.086 81 38 0 30 110 10 90 1.03e-24 92.4
MsG0580025957.01.T02 MTR_5g022720 69.231 65 20 0 98 162 1 65 6.78e-24 94.7
MsG0580025957.01.T02 MTR_7g063280 38.679 106 61 2 51 154 72 175 7.83e-24 92.0
MsG0580025957.01.T02 MTR_8g036780 50.617 81 40 0 29 109 12 92 9.78e-24 90.1
MsG0580025957.01.T02 MTR_5g034450 53.947 76 35 0 35 110 33 108 1.07e-23 90.1
MsG0580025957.01.T02 MTR_2g017550 53.333 75 35 0 29 103 86 160 1.28e-23 91.3
MsG0580025957.01.T02 MTR_1g081930 39.655 116 70 0 27 142 14 129 1.93e-23 90.1
MsG0580025957.01.T02 MTR_5g085210 37.879 132 66 1 30 161 98 213 2.08e-23 92.4
MsG0580025957.01.T02 MTR_8g031800 53.846 78 36 0 33 110 13 90 4.76e-23 88.6
MsG0580025957.01.T02 MTR_1g045340 35.766 137 84 1 11 147 48 180 9.06e-23 89.7
MsG0580025957.01.T02 MTR_4g094272 51.765 85 37 2 17 98 57 140 9.06e-23 88.6
MsG0580025957.01.T02 MTR_2g047100 43.478 115 50 1 36 150 3 102 9.22e-23 87.4
MsG0580025957.01.T02 MTR_1g074090 45.161 93 51 0 18 110 48 140 1.24e-22 88.6
MsG0580025957.01.T02 MTR_8g031080 39.716 141 59 4 21 161 259 373 1.99e-22 92.4
MsG0580025957.01.T02 MTR_1g034430 39.815 108 65 0 30 137 74 181 2.96e-22 88.2
MsG0580025957.01.T02 MTR_1g054905 55.263 76 34 0 19 94 69 144 8.54e-22 86.7
MsG0580025957.01.T02 MTR_0015s0120 57.333 75 32 0 36 110 278 352 1.18e-21 90.1
MsG0580025957.01.T02 MTR_2g088140 81.250 48 9 0 115 162 52 99 1.45e-21 84.7
MsG0580025957.01.T02 MTR_7g006380 35.821 134 59 1 28 161 43 149 1.83e-21 86.3
MsG0580025957.01.T02 MTR_2g059470 47.500 80 42 0 55 134 4 83 2.78e-21 82.8
MsG0580025957.01.T02 MTR_8g022750 37.398 123 60 1 28 150 182 287 3.27e-21 87.8
MsG0580025957.01.T02 MTR_8g079540 40.000 100 60 0 18 117 15 114 1.33e-20 82.4
MsG0580025957.01.T02 MTR_6g034915 33.333 156 57 2 36 161 10 148 2.13e-20 83.2
MsG0580025957.01.T02 MTR_7g078610 56.061 66 29 0 28 93 28 93 2.54e-20 80.9
MsG0580025957.01.T02 MTR_1g050488 48.000 75 39 0 36 110 83 157 3.60e-20 82.4
MsG0580025957.01.T02 MTR_1g022215 50.000 68 34 0 38 105 1 68 3.85e-20 80.1
MsG0580025957.01.T02 MTR_4g064590 45.161 93 45 2 71 161 46 134 1.96e-19 80.5
MsG0580025957.01.T02 MTR_8g028785 35.507 138 65 1 21 158 252 365 3.03e-19 83.6
MsG0580025957.01.T02 MTR_8g059135 38.095 105 64 1 41 145 19 122 5.05e-19 78.6
MsG0580025957.01.T02 MTR_6g033225 47.368 76 40 0 35 110 54 129 9.25e-19 78.2
MsG0580025957.01.T02 MTR_1g099430 45.455 88 46 1 22 107 96 183 9.30e-19 79.7
MsG0580025957.01.T02 MTR_8g038340 44.444 81 45 0 28 108 166 246 2.05e-18 79.7
MsG0580025957.01.T02 MTR_4g008270 46.914 81 43 0 52 132 11 91 2.06e-18 75.9
MsG0580025957.01.T02 MTR_6g016355 44.156 77 43 0 38 114 103 179 1.75e-17 75.9
MsG0580025957.01.T02 MTR_3g462490 30.000 160 57 2 8 167 62 166 2.01e-17 78.6
MsG0580025957.01.T02 MTR_1g018300 44.545 110 53 3 30 138 42 144 5.41e-17 75.9
MsG0580025957.01.T02 MTR_1g074240 49.231 65 33 0 55 119 4 68 1.19e-16 70.9
MsG0580025957.01.T02 MTR_1g109000 41.975 81 47 0 30 110 5 85 1.55e-16 70.9
MsG0580025957.01.T02 MTR_6g045427 35.714 112 44 1 28 139 150 233 4.26e-16 73.2
MsG0580025957.01.T02 MTR_2g055490 40.566 106 30 1 56 161 12 84 6.83e-16 70.1
MsG0580025957.01.T02 MTR_5g042170 70.455 44 13 0 30 73 29 72 1.36e-15 69.3
MsG0580025957.01.T02 MTR_4g046027 53.968 63 29 0 31 93 107 169 5.82e-15 68.9
MsG0580025957.01.T02 MTR_7g053400 34.545 110 38 1 52 161 176 251 8.56e-15 70.1
MsG0580025957.01.T02 MTR_3g064060 51.923 52 25 0 20 71 6 57 6.16e-14 63.2
MsG0580025957.01.T02 MTR_8g069135 47.887 71 32 1 18 83 156 226 7.73e-14 68.2
MsG0580025957.01.T02 MTR_4g128080 50.000 60 30 0 102 161 5 64 1.47e-13 62.8
MsG0580025957.01.T02 MTR_8g045330 37.895 95 51 1 7 93 45 139 1.89e-13 64.7
MsG0580025957.01.T02 MTR_1g045560 37.143 105 50 2 57 161 1 89 3.39e-13 62.4
MsG0580025957.01.T02 MTR_6g023630 57.143 49 20 1 65 112 3 51 4.31e-13 62.0
MsG0580025957.01.T02 MTR_5g068115 58.140 43 18 0 29 71 12 54 1.10e-12 60.5
MsG0580025957.01.T02 MTR_8g088220 39.474 76 46 0 61 136 4 79 7.15e-12 58.5
MsG0580025957.01.T02 MTR_8g036690 34.884 86 40 1 82 167 2 71 8.17e-12 58.2
MsG0580025957.01.T02 MTR_1g087480 43.939 66 37 0 18 83 155 220 3.59e-11 59.7
MsG0580025957.01.T02 MTR_5g430460 50.909 55 27 0 23 77 190 244 5.36e-11 59.7
MsG0580025957.01.T02 MTR_1964s0010 50.000 46 23 0 29 74 47 92 7.92e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAAATCGACGTTGTCAAAT+TGG 0.231105 5:-28023965 None:intergenic
CCGTTAAACAGAACAGTTAT+TGG 0.257202 5:+28023816 MsG0580025957.01.T03:CDS
CCTGAACCACTTTGTCTTAC+CGG 0.311943 5:-28023687 None:intergenic
CCAATAACTGTTCTGTTTAA+CGG 0.326895 5:-28023816 None:intergenic
GAGGTAGCTCACACCCTTGC+TGG 0.335947 5:+28024134 MsG0580025957.01.T03:CDS
CATTAACCCAACGATGGTTA+AGG 0.391523 5:-28024016 None:intergenic
CACAAACTTCTCAAACTCTA+AGG 0.397546 5:+28024085 MsG0580025957.01.T03:CDS
CCGGTAAGACAAAGTGGTTC+AGG 0.409527 5:+28023687 MsG0580025957.01.T02:CDS
CCTATGTGTGTTGTTGCTAT+AGG 0.419768 5:+28023897 MsG0580025957.01.T03:CDS
GATGTCACTTAGCCATTGTA+AGG 0.424948 5:-28023943 None:intergenic
ATTAACCCAACGATGGTTAA+GGG 0.438806 5:-28024015 None:intergenic
CATATGCCCTTAACCATCGT+TGG 0.479099 5:+28024009 MsG0580025957.01.T03:CDS
AGGTAGCTCACACCCTTGCT+GGG 0.481440 5:+28024135 MsG0580025957.01.T03:CDS
ACTCTAAGGTCGAGTTTGAC+AGG 0.487784 5:+28024099 MsG0580025957.01.T03:CDS
CACTTAGCCATTGTAAGGCA+TGG 0.506485 5:-28023938 None:intergenic
ATATGCCCTTAACCATCGTT+GGG 0.509276 5:+28024010 MsG0580025957.01.T03:CDS
TGACAACGTCGATTTCGTCT+TGG 0.515739 5:+28023971 MsG0580025957.01.T03:CDS
AGTTTGTGTTAAAGAGACTC+TGG 0.543874 5:-28024070 None:intergenic
TGACTTCGGTCAGGTGGCAG+CGG 0.550323 5:+28023745 MsG0580025957.01.T03:CDS
GGTAGCTCACACCCTTGCTG+GGG 0.553976 5:+28024136 MsG0580025957.01.T03:CDS
TGACAGGAGACAAGCAAATG+AGG 0.554815 5:+28024115 MsG0580025957.01.T03:CDS
TCTGGCATGCTGAAATCACT+TGG 0.580643 5:-28024052 None:intergenic
GCTTATTCCATGCCTTACAA+TGG 0.586368 5:+28023931 MsG0580025957.01.T03:CDS
CGAGTGATATGACTTCGGTC+AGG 0.587084 5:+28023736 MsG0580025957.01.T02:CDS
CAAATGTTCCTTCGTCATCA+CGG 0.607979 5:-28023848 None:intergenic
ATTGGGACTAGCTGATAATG+TGG 0.608869 5:-28024163 None:intergenic
CCTATAGCAACAACACACAT+AGG 0.610860 5:-28023897 None:intergenic
GTAGCTCACACCCTTGCTGG+GGG 0.613477 5:+28024137 MsG0580025957.01.T03:CDS
AAAATACCGGTAAGACAAAG+TGG 0.615623 5:+28023681 MsG0580025957.01.T02:CDS
GTGATATGACTTCGGTCAGG+TGG 0.626291 5:+28023739 MsG0580025957.01.T02:CDS
TGACGAAGGAACATTTGTGC+TGG 0.634598 5:+28023854 MsG0580025957.01.T03:CDS
TACAACGAGTGATATGACTT+CGG 0.664594 5:+28023731 MsG0580025957.01.T02:CDS
CTGTAACATTAACCCAACGA+TGG 0.670424 5:-28024022 None:intergenic
AGGTGGCAGCGGCCACAACG+AGG 0.707158 5:+28023756 MsG0580025957.01.T03:CDS
ATATGTGTCCGTGATGACGA+AGG 0.756014 5:+28023840 MsG0580025957.01.T03:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATTGATGATTCGTTTT+CGG + Chr5:28023791-28023810 MsG0580025957.01.T03:CDS 20.0%
!!! CATCAATATTACTTTTCAGA+CGG - Chr5:28023782-28023801 None:intergenic 25.0%
CCAATAACTGTTCTGTTTAA+CGG - Chr5:28023819-28023838 None:intergenic 30.0%
AAAATACCGGTAAGACAAAG+TGG + Chr5:28023681-28023700 MsG0580025957.01.T02:CDS 35.0%
AGTTTGTGTTAAAGAGACTC+TGG - Chr5:28024073-28024092 None:intergenic 35.0%
ATTAACCCAACGATGGTTAA+GGG - Chr5:28024018-28024037 None:intergenic 35.0%
CACAAACTTCTCAAACTCTA+AGG + Chr5:28024085-28024104 MsG0580025957.01.T03:CDS 35.0%
CCGTTAAACAGAACAGTTAT+TGG + Chr5:28023816-28023835 MsG0580025957.01.T03:CDS 35.0%
TACAACGAGTGATATGACTT+CGG + Chr5:28023731-28023750 MsG0580025957.01.T02:CDS 35.0%
ATATGCCCTTAACCATCGTT+GGG + Chr5:28024010-28024029 MsG0580025957.01.T03:CDS 40.0%
CAAATGTTCCTTCGTCATCA+CGG - Chr5:28023851-28023870 None:intergenic 40.0%
CATTAACCCAACGATGGTTA+AGG - Chr5:28024019-28024038 None:intergenic 40.0%
CCTATAGCAACAACACACAT+AGG - Chr5:28023900-28023919 None:intergenic 40.0%
CGAAATCGACGTTGTCAAAT+TGG - Chr5:28023968-28023987 None:intergenic 40.0%
CTGTAACATTAACCCAACGA+TGG - Chr5:28024025-28024044 None:intergenic 40.0%
GCTTATTCCATGCCTTACAA+TGG + Chr5:28023931-28023950 MsG0580025957.01.T03:CDS 40.0%
! CCTATGTGTGTTGTTGCTAT+AGG + Chr5:28023897-28023916 MsG0580025957.01.T03:CDS 40.0%
! GATGTCACTTAGCCATTGTA+AGG - Chr5:28023946-28023965 None:intergenic 40.0%
CACTTAGCCATTGTAAGGCA+TGG - Chr5:28023941-28023960 None:intergenic 45.0%
CATATGCCCTTAACCATCGT+TGG + Chr5:28024009-28024028 MsG0580025957.01.T03:CDS 45.0%
CCTGAACCACTTTGTCTTAC+CGG - Chr5:28023690-28023709 None:intergenic 45.0%
TCTGGCATGCTGAAATCACT+TGG - Chr5:28024055-28024074 None:intergenic 45.0%
TGACAGGAGACAAGCAAATG+AGG + Chr5:28024115-28024134 MsG0580025957.01.T03:CDS 45.0%
TGACGAAGGAACATTTGTGC+TGG + Chr5:28023854-28023873 MsG0580025957.01.T03:CDS 45.0%
! ATATGTGTCCGTGATGACGA+AGG + Chr5:28023840-28023859 MsG0580025957.01.T03:CDS 45.0%
!! ACTCTAAGGTCGAGTTTGAC+AGG + Chr5:28024099-28024118 MsG0580025957.01.T03:CDS 45.0%
!! TGACAACGTCGATTTCGTCT+TGG + Chr5:28023971-28023990 MsG0580025957.01.T03:CDS 45.0%
CCGGTAAGACAAAGTGGTTC+AGG + Chr5:28023687-28023706 MsG0580025957.01.T02:CDS 50.0%
CGAGTGATATGACTTCGGTC+AGG + Chr5:28023736-28023755 MsG0580025957.01.T02:CDS 50.0%
GTGATATGACTTCGGTCAGG+TGG + Chr5:28023739-28023758 MsG0580025957.01.T02:CDS 50.0%
AATGTGGCAACCCCCAGCAA+GGG - Chr5:28024150-28024169 None:intergenic 55.0%
AGGTAGCTCACACCCTTGCT+GGG + Chr5:28024135-28024154 MsG0580025957.01.T03:CDS 55.0%
TAATGTGGCAACCCCCAGCA+AGG - Chr5:28024151-28024170 None:intergenic 55.0%
! CTTTTCAGACGGCCTCGTTG+TGG - Chr5:28023771-28023790 None:intergenic 55.0%
GAGGTAGCTCACACCCTTGC+TGG + Chr5:28024134-28024153 MsG0580025957.01.T03:CDS 60.0%
GGTAGCTCACACCCTTGCTG+GGG + Chr5:28024136-28024155 MsG0580025957.01.T03:CDS 60.0%
GTAGCTCACACCCTTGCTGG+GGG + Chr5:28024137-28024156 MsG0580025957.01.T03:CDS 60.0%
TGACTTCGGTCAGGTGGCAG+CGG + Chr5:28023745-28023764 MsG0580025957.01.T03:CDS 60.0%
AGGTGGCAGCGGCCACAACG+AGG + Chr5:28023756-28023775 MsG0580025957.01.T03:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 28023672 28024178 28023672 ID=MsG0580025957.01;Name=MsG0580025957.01
Chr5 mRNA 28023744 28024178 28023744 ID=MsG0580025957.01.T01;Parent=MsG0580025957.01;Name=MsG0580025957.01.T01;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|0|0|2|0|115
Chr5 exon 28023949 28024178 28023949 ID=MsG0580025957.01.T01:exon:29037;Parent=MsG0580025957.01.T01
Chr5 exon 28023744 28023861 28023744 ID=MsG0580025957.01.T01:exon:29036;Parent=MsG0580025957.01.T01
Chr5 CDS 28023744 28023861 28023744 ID=MsG0580025957.01.T01:cds;Parent=MsG0580025957.01.T01
Chr5 CDS 28023949 28024178 28023949 ID=MsG0580025957.01.T01:cds;Parent=MsG0580025957.01.T01
Chr5 mRNA 28023672 28024178 28023672 ID=MsG0580025957.01.T02;Parent=MsG0580025957.01;Name=MsG0580025957.01.T02;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|0|1|0|168
Chr5 exon 28023672 28024178 28023672 ID=MsG0580025957.01.T02:exon:29038;Parent=MsG0580025957.01.T02
Chr5 CDS 28023672 28024178 28023672 ID=MsG0580025957.01.T02:cds;Parent=MsG0580025957.01.T02
Chr5 mRNA 28023744 28024178 28023744 ID=MsG0580025957.01.T03;Parent=MsG0580025957.01;Name=MsG0580025957.01.T03;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|0|1|0|144
Chr5 exon 28023744 28024178 28023744 ID=MsG0580025957.01.T03:exon:29039;Parent=MsG0580025957.01.T03
Chr5 CDS 28023744 28024178 28023744 ID=MsG0580025957.01.T03:cds;Parent=MsG0580025957.01.T03
Gene Sequence

>MsG0580025957.01.T01

ATGACTTCGGTCAGGTGGCAGCGGCCACAACGAGGCCGTCTGAAAAGTAATATTGATGATTCGTTTTCGGATCCGTTAAACAGAACAGTTATTGGCATATGTGTCCGTGATGACGAAGAATGGCTAAGTGACATCCAATTTGACAACGTCGATTTCGTCTTGGATTCAAAGATTACAACATATGCCCTTAACCATCGTTGGGTTAATGTTACAGATTTTTGCCAAGTGATTTCAGCATGCCAGAGTCTCTTTAACACAAACTTCTCAAACTCTAAGGTCGAGTTTGACAGGAGACAAGCAAATGAGGTAGCTCACACCCTTGCTGGGGGTTGCCACATTATCAGCTAG

>MsG0580025957.01.T03

ATGACTTCGGTCAGGTGGCAGCGGCCACAACGAGGCCGTCTGAAAAGTAATATTGATGATTCGTTTTCGGATCCGTTAAACAGAACAGTTATTGGCATATGTGTCCGTGATGACGAAGGAACATTTGTGCTGGCTAAAACTGTATCTATCTCTCCTATGTGTGTTGTTGCTATAGGAGAAGCTCTAAGCTTATTCCATGCCTTACAATGGCTAAGTGACATCCAATTTGACAACGTCGATTTCGTCTTGGATTCAAAGATTACAACATATGCCCTTAACCATCGTTGGGTTAATGTTACAGATTTTTGCCAAGTGATTTCAGCATGCCAGAGTCTCTTTAACACAAACTTCTCAAACTCTAAGGTCGAGTTTGACAGGAGACAAGCAAATGAGGTAGCTCACACCCTTGCTGGGGGTTGCCACATTATCAGCTAG

>MsG0580025957.01.T02

ATGACGACAAAAATACCGGTAAGACAAAGTGGTTCAGGTACAACTCAAAATTCTTCTGCTACAACGAGTGATATGACTTCGGTCAGGTGGCAGCGGCCACAACGAGGCCGTCTGAAAAGTAATATTGATGATTCGTTTTCGGATCCGTTAAACAGAACAGTTATTGGCATATGTGTCCGTGATGACGAAGGAACATTTGTGCTGGCTAAAACTGTATCTATCTCTCCTATGTGTGTTGTTGCTATAGGAGAAGCTCTAAGCTTATTCCATGCCTTACAATGGCTAAGTGACATCCAATTTGACAACGTCGATTTCGTCTTGGATTCAAAGATTACAACATATGCCCTTAACCATCGTTGGGTTAATGTTACAGATTTTTGCCAAGTGATTTCAGCATGCCAGAGTCTCTTTAACACAAACTTCTCAAACTCTAAGGTCGAGTTTGACAGGAGACAAGCAAATGAGGTAGCTCACACCCTTGCTGGGGGTTGCCACATTATCAGCTAG
Protein sequence

>MsG0580025957.01.T01

MTSVRWQRPQRGRLKSNIDDSFSDPLNRTVIGICVRDDEEWLSDIQFDNVDFVLDSKITTYALNHRWVNVTDFCQVISACQSLFNTNFSNSKVEFDRRQANEVAHTLAGGCHIIS*

>MsG0580025957.01.T03

MTSVRWQRPQRGRLKSNIDDSFSDPLNRTVIGICVRDDEGTFVLAKTVSISPMCVVAIGEALSLFHALQWLSDIQFDNVDFVLDSKITTYALNHRWVNVTDFCQVISACQSLFNTNFSNSKVEFDRRQANEVAHTLAGGCHIIS*

>MsG0580025957.01.T02

MTTKIPVRQSGSGTTQNSSATTSDMTSVRWQRPQRGRLKSNIDDSFSDPLNRTVIGICVRDDEGTFVLAKTVSISPMCVVAIGEALSLFHALQWLSDIQFDNVDFVLDSKITTYALNHRWVNVTDFCQVISACQSLFNTNFSNSKVEFDRRQANEVAHTLAGGCHIIS*