AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026143.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026143.01.T01 MTR_5g040320 98.099 263 5 0 1 263 1 263 0.0 537
MsG0580026143.01.T01 MTR_1g097310 67.200 250 79 2 9 258 8 254 1.85e-125 357
MsG0580026143.01.T01 MTR_1g097320 63.704 135 49 0 76 210 1 135 1.03e-60 189
MsG0580026143.01.T01 MTR_6g039970 27.354 223 143 9 39 253 52 263 1.91e-13 68.9
MsG0580026143.01.T01 MTR_8g063210 27.488 211 135 8 39 242 52 251 2.51e-13 68.6
MsG0580026143.01.T01 MTR_5g035190 28.251 223 141 9 39 253 52 263 5.58e-13 67.4
MsG0580026143.01.T01 MTR_5g035190 29.557 203 127 8 59 253 15 209 7.72e-13 66.2
MsG0580026143.01.T01 MTR_4g102060 31.788 151 94 4 101 247 129 274 8.96e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026143.01.T01 AT1G14290 77.255 255 57 1 9 263 6 259 4.68e-153 427
MsG0580026143.01.T01 AT1G69640 77.395 261 58 1 3 263 1 260 3.06e-144 405
MsG0580026143.01.T01 AT1G14290 82.065 184 33 0 80 263 2 185 7.32e-116 330
MsG0580026143.01.T01 AT2G29390 26.818 220 135 8 36 238 49 259 2.84e-12 65.5

Find 105 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTCTTGTTCGCATGATTC+TGG 0.120303 5:+31308283 MsG0580026143.01.T01:five_prime_UTR
CCAATATTTGCATCAATTTC+AGG 0.147675 5:-31308548 None:intergenic
TTGATTATTGTAATGGTAAA+TGG 0.188606 5:+31310976 MsG0580026143.01.T01:three_prime_UTR
CCTGAAATTGATGCAAATAT+TGG 0.198937 5:+31308548 MsG0580026143.01.T01:five_prime_UTR
TCTTCTCTTCTCTCTCTCTT+TGG 0.206818 5:+31308340 MsG0580026143.01.T01:five_prime_UTR
AATGATGATGGATTTGGATT+TGG 0.215936 5:+31308638 MsG0580026143.01.T01:exon
TCTAAGTTTGATGTTGTTAA+AGG 0.231277 5:+31308801 MsG0580026143.01.T01:CDS
ATGATGATGGATTTGGATTT+GGG 0.233833 5:+31308639 MsG0580026143.01.T01:CDS
AGATGAGGATGAAAAGAATT+TGG 0.239236 5:+31308776 MsG0580026143.01.T01:CDS
TTAGTTTATTGGATTTATTC+TGG 0.243045 5:+31308699 MsG0580026143.01.T01:CDS
CAAACTCAACAACAATTCTT+TGG 0.258691 5:-31310956 None:intergenic
TCCCTTGTTCCTTCAAGGTT+TGG 0.259243 5:-31308453 None:intergenic
TCTCTTCCCTTGTTCCTTCA+AGG 0.271851 5:-31308458 None:intergenic
GGGGTTTCTGATGAGATGTT+AGG 0.282336 5:+31308660 MsG0580026143.01.T01:CDS
GTTTGTTTGCTGAAGATTAT+AGG 0.288420 5:+31308739 MsG0580026143.01.T01:CDS
ATGAGAAGGGAATTAAATAT+TGG 0.310598 5:-31308394 None:intergenic
AGGGAATTAAATATTGGATT+AGG 0.323539 5:-31308388 None:intergenic
GTGAGAAATGATGATGGATT+TGG 0.328614 5:+31308632 MsG0580026143.01.T01:exon
CTGAAATTGATGCAAATATT+GGG 0.332540 5:+31308549 MsG0580026143.01.T01:five_prime_UTR
CTCATTGTACCTTATTCGTT+TGG 0.338165 5:+31310177 MsG0580026143.01.T01:CDS
CGCAACCGTTCTTTGTTATG+TGG 0.347193 5:+31310442 MsG0580026143.01.T01:CDS
TATAGAGCTCCAAACGAATA+AGG 0.355458 5:-31310186 None:intergenic
TAGGACTGTTAGCTATTATA+TGG 0.367303 5:+31310735 MsG0580026143.01.T01:three_prime_UTR
TGTTATGTGGGACAAAATCT+TGG 0.373501 5:+31310455 MsG0580026143.01.T01:CDS
GCTCTCTTCTCTAATGAGTA+TGG 0.377222 5:-31310489 None:intergenic
ACTTGTTGCCGTAGAGTTGA+TGG 0.381170 5:-31310410 None:intergenic
AACATATGGAATAGGTTTCC+AGG 0.385390 5:-31310354 None:intergenic
ATTACTCATTCATCCTTGTA+AGG 0.397174 5:-31310543 None:intergenic
TCCTATTCTATTGAAGTGTT+CGG 0.398810 5:+31310679 MsG0580026143.01.T01:three_prime_UTR
ATATTTAATTCCCTTCTCAT+TGG 0.405071 5:+31308397 MsG0580026143.01.T01:five_prime_UTR
AAAGTCTGCAAAACAAATAA+AGG 0.406473 5:-31310889 None:intergenic
TATTACTGCAATGTTGGTAA+TGG 0.409424 5:+31310074 MsG0580026143.01.T01:CDS
TTCAATCATCCAAACCTTGA+AGG 0.417786 5:+31308444 MsG0580026143.01.T01:five_prime_UTR
GGGTTTCTGATGAGATGTTA+GGG 0.418518 5:+31308661 MsG0580026143.01.T01:CDS
TATTCTCATTGGTACTACTT+TGG 0.419651 5:-31310023 None:intergenic
GGTTAGGGTTAAAGATTAAG+TGG 0.431743 5:+31308503 MsG0580026143.01.T01:five_prime_UTR
ACAATTTATTACTGCAATGT+TGG 0.438454 5:+31310068 MsG0580026143.01.T01:CDS
ATCATATGTAATGTATATAC+AGG 0.447628 5:-31310654 None:intergenic
CACTGTGGGTTATGGCTCCC+TGG 0.456037 5:+31310336 MsG0580026143.01.T01:CDS
GTTGAGTTTGATTATTGTAA+TGG 0.458477 5:+31310969 MsG0580026143.01.T01:three_prime_UTR
TTGGAACCTTCCTCCTTCTG+TGG 0.459208 5:+31308359 MsG0580026143.01.T01:five_prime_UTR
GTTATTTATAAATCAAATCA+AGG 0.460379 5:+31308580 MsG0580026143.01.T01:five_prime_UTR
TAAATCCAATAAACTAAAAG+TGG 0.460414 5:-31308693 None:intergenic
GTTATGTGGGACAAAATCTT+GGG 0.464199 5:+31310456 MsG0580026143.01.T01:CDS
ACAAGAACACAAATGATTTG+TGG 0.465734 5:-31311079 None:intergenic
GTCGTTAAGAAGCCCCTCAA+TGG 0.476386 5:-31310217 None:intergenic
TGGATGACCACTGTGGGTTA+TGG 0.479214 5:+31310328 MsG0580026143.01.T01:CDS
TACATGTACACACGATAACT+TGG 0.482247 5:+31308226 None:intergenic
GAGAGTGAATGTGTTTGTAT+AGG 0.484907 5:-31310143 None:intergenic
TCATCCTTGTAAGGTTTACA+AGG 0.487494 5:-31310534 None:intergenic
TCATTTATGATTTAATGTGA+TGG 0.490850 5:+31311035 MsG0580026143.01.T01:three_prime_UTR
CTATACAATCATCCCATTGA+GGG 0.494387 5:+31310204 MsG0580026143.01.T01:CDS
CACTCTCCGGCATGGAAGCT+AGG 0.519933 5:+31310716 MsG0580026143.01.T01:three_prime_UTR
AAGGAGAGAAGTATTCTCAT+TGG 0.524581 5:-31310034 None:intergenic
TTGTGTATCTTTGTTACCAC+GGG 0.526527 5:-31310779 None:intergenic
GCAACCGTTCTTTGTTATGT+GGG 0.527794 5:+31310443 MsG0580026143.01.T01:CDS
TCGTTAAGAAGCCCCTCAAT+GGG 0.530146 5:-31310216 None:intergenic
AAAACTTACTGCAAACAAAA+GGG 0.530834 5:-31308862 None:intergenic
TCCGAACACTTCAATAGAAT+AGG 0.540732 5:-31310680 None:intergenic
TCAACAAGCTGTTCAAGCTG+TGG 0.543673 5:+31308833 MsG0580026143.01.T01:CDS
ATCCAAACCTTGAAGGAACA+AGG 0.549016 5:+31308451 MsG0580026143.01.T01:five_prime_UTR
CTCGAGGACTCATACCAGAT+AGG 0.552424 5:-31310266 None:intergenic
AAAACCTAAAACCAATGAGA+AGG 0.552512 5:-31308408 None:intergenic
CTCTAATGAGTATGGCATGT+AGG 0.553171 5:-31310481 None:intergenic
TAGACCTTGTAAACCTTACA+AGG 0.553677 5:+31310530 MsG0580026143.01.T01:CDS
ACCACGTCGAAACCACTCTC+CGG 0.555610 5:+31310703 MsG0580026143.01.T01:three_prime_UTR
GGGTTTGTGAGAAATGATGA+TGG 0.559691 5:+31308626 MsG0580026143.01.T01:exon
TTGTCCCACATAACAAAGAA+CGG 0.560325 5:-31310447 None:intergenic
GATAGTATCAAAGATTGCAT+AGG 0.560873 5:-31310861 None:intergenic
TGTATCTTTGTTACCACGGG+CGG 0.564341 5:-31310776 None:intergenic
TCTATACAATCATCCCATTG+AGG 0.565015 5:+31310203 MsG0580026143.01.T01:CDS
ACATATGGAATAGGTTTCCA+GGG 0.566540 5:-31310353 None:intergenic
TGATGATGGATTTGGATTTG+GGG 0.566623 5:+31308640 MsG0580026143.01.T01:CDS
CTAGCTTCCATGCCGGAGAG+TGG 0.575579 5:-31310715 None:intergenic
ATAAATGATTATAAGTGTCA+TGG 0.577041 5:-31311020 None:intergenic
CGGTGCTCGTCATCATAACG+TGG 0.579071 5:-31310619 None:intergenic
TCCAGAATCATGCGAACAAG+AGG 0.580127 5:-31308284 None:intergenic
ATTGTGTATCTTTGTTACCA+CGG 0.580568 5:-31310780 None:intergenic
GTCGAAACCACTCTCCGGCA+TGG 0.584611 5:+31310708 MsG0580026143.01.T01:three_prime_UTR
GATGATGGATTTGGATTTGG+GGG 0.591163 5:+31308641 MsG0580026143.01.T01:CDS
AAACCTAAAACCAATGAGAA+GGG 0.596497 5:-31308407 None:intergenic
GTATCTTTGTTACCACGGGC+GGG 0.596937 5:-31310775 None:intergenic
TCATTAGAGAAGAGAGCTAG+TGG 0.601615 5:+31310495 MsG0580026143.01.T01:CDS
GATGTACACCATCAACTCTA+CGG 0.605436 5:+31310402 MsG0580026143.01.T01:CDS
AACAGTCCTAGCTTCCATGC+CGG 0.605863 5:-31310722 None:intergenic
TATACAGGCATGATCACTCT+CGG 0.620105 5:-31310639 None:intergenic
CAATGTTGGTAATGGACACA+TGG 0.623258 5:+31310082 MsG0580026143.01.T01:CDS
AAAAGATAAAGCTCCACCGA+TGG 0.624769 5:-31310241 None:intergenic
TAAATTGTCTTGCTAAAACA+AGG 0.626413 5:-31310053 None:intergenic
CTTTGCCACCATTAAAACCG+TGG 0.628473 5:+31310308 MsG0580026143.01.T01:CDS
TCCAAACCTTGAAGGAACAA+GGG 0.634614 5:+31308452 MsG0580026143.01.T01:five_prime_UTR
TCGGACAATCATGTGTGTAA+CGG 0.634956 5:-31308253 None:intergenic
AAACCGTGGATGACCACTGT+GGG 0.635025 5:+31310322 MsG0580026143.01.T01:CDS
GGGCTTCTTAACGACACCAT+CGG 0.641083 5:+31310225 MsG0580026143.01.T01:CDS
TAACCCACAGTGGTCATCCA+CGG 0.641389 5:-31310325 None:intergenic
CTTCTTAACGACACCATCGG+TGG 0.644710 5:+31310228 MsG0580026143.01.T01:CDS
CCAGAATCATGCGAACAAGA+GGG 0.651890 5:-31308283 None:intergenic
TATACAATCATCCCATTGAG+GGG 0.661307 5:+31310205 MsG0580026143.01.T01:CDS
GCCGGAGAGTGGTTTCGACG+TGG 0.663848 5:-31310704 None:intergenic
GGTGCTCGTCATCATAACGT+GGG 0.688355 5:-31310618 None:intergenic
AGAGTTGATGGTGTACATCG+TGG 0.699589 5:-31310398 None:intergenic
CGTTTGATGTATCCCGCCCG+TGG 0.704892 5:+31310763 MsG0580026143.01.T01:three_prime_UTR
TTAGAGAAGAGAGCTAGTGG+CGG 0.706802 5:+31310498 MsG0580026143.01.T01:CDS
AAAACCGTGGATGACCACTG+TGG 0.720859 5:+31310321 MsG0580026143.01.T01:CDS
CAGGGAGCCATAACCCACAG+TGG 0.760631 5:-31310335 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAAATAATTTCAATTTTAA+GGG + Chr5:31308482-31308501 MsG0580026143.01.T01:five_prime_UTR 10.0%
!! GTTATTTATAAATCAAATCA+AGG + Chr5:31308580-31308599 MsG0580026143.01.T01:five_prime_UTR 15.0%
!!! AATTGTTATTGAAAAACATA+TGG - Chr5:31310371-31310390 None:intergenic 15.0%
!!! AGTTTTAATAAAGAATATTG+AGG + Chr5:31310833-31310852 MsG0580026143.01.T01:three_prime_UTR 15.0%
!!! ATTTTTTCTGATTTTTGAAA+CGG - Chr5:31309585-31309604 None:intergenic 15.0%
!!! GAGAAATAATTTCAATTTTA+AGG + Chr5:31308481-31308500 MsG0580026143.01.T01:five_prime_UTR 15.0%
!!! TTACTAGTAGATATTTTTTA+CGG + Chr5:31308901-31308920 MsG0580026143.01.T01:intron 15.0%
!! ATAAATGATTATAAGTGTCA+TGG - Chr5:31311023-31311042 None:intergenic 20.0%
!! ATCATATGTAATGTATATAC+AGG - Chr5:31310657-31310676 None:intergenic 20.0%
!! CAAAAATCAAAACACATAAA+GGG - Chr5:31309135-31309154 None:intergenic 20.0%
!! TAAAAAAGACAAAACACAAA+AGG - Chr5:31309193-31309212 None:intergenic 20.0%
!! TAAATCCAATAAACTAAAAG+TGG - Chr5:31308696-31308715 None:intergenic 20.0%
!! TATTGAAAAACATATGGAAT+AGG - Chr5:31310365-31310384 None:intergenic 20.0%
!! TCAAAAATCAAAACACATAA+AGG - Chr5:31309136-31309155 None:intergenic 20.0%
!! TCATTTATGATTTAATGTGA+TGG + Chr5:31311035-31311054 MsG0580026143.01.T01:three_prime_UTR 20.0%
!! TGTATAACATATTGTCATTA+AGG - Chr5:31309475-31309494 None:intergenic 20.0%
!! TTAGTTTATTGGATTTATTC+TGG + Chr5:31308699-31308718 MsG0580026143.01.T01:CDS 20.0%
!! TTGATTATTGTAATGGTAAA+TGG + Chr5:31310976-31310995 MsG0580026143.01.T01:three_prime_UTR 20.0%
!!! AAAGATTTGATTTTTTCATC+TGG + Chr5:31308605-31308624 MsG0580026143.01.T01:five_prime_UTR 20.0%
!!! AAGATTTGATTTTTTCATCT+GGG + Chr5:31308606-31308625 MsG0580026143.01.T01:five_prime_UTR 20.0%
!!! AATTTCAATTTTAAGGGTTA+GGG + Chr5:31308488-31308507 MsG0580026143.01.T01:five_prime_UTR 20.0%
!!! TAATTTCAATTTTAAGGGTT+AGG + Chr5:31308487-31308506 MsG0580026143.01.T01:five_prime_UTR 20.0%
!!! TTGAAAATTATTTTGTTTGC+AGG - Chr5:31308311-31308330 None:intergenic 20.0%
!!! TTTATGTGTTTTGATTTTTG+AGG + Chr5:31309135-31309154 MsG0580026143.01.T01:intron 20.0%
! AAAAACTTACTGCAAACAAA+AGG - Chr5:31308866-31308885 None:intergenic 25.0%
! AAAACTTACTGCAAACAAAA+GGG - Chr5:31308865-31308884 None:intergenic 25.0%
! AAACATAGTATTTGCAAAAG+AGG - Chr5:31309227-31309246 None:intergenic 25.0%
! AAAGTCTGCAAAACAAATAA+AGG - Chr5:31310892-31310911 None:intergenic 25.0%
! AACATAGTATTTGCAAAAGA+GGG - Chr5:31309226-31309245 None:intergenic 25.0%
! ACAATTTATTACTGCAATGT+TGG + Chr5:31310068-31310087 MsG0580026143.01.T01:CDS 25.0%
! AGGGAATTAAATATTGGATT+AGG - Chr5:31308391-31308410 None:intergenic 25.0%
! AGTAAAATCAACACAAGATT+TGG - Chr5:31309687-31309706 None:intergenic 25.0%
! ATATTTAATTCCCTTCTCAT+TGG + Chr5:31308397-31308416 MsG0580026143.01.T01:five_prime_UTR 25.0%
! ATGAGAAGGGAATTAAATAT+TGG - Chr5:31308397-31308416 None:intergenic 25.0%
! CTATAACTATAACTAAACTG+TGG + Chr5:31309961-31309980 MsG0580026143.01.T01:intron 25.0%
! CTGAAATTGATGCAAATATT+GGG + Chr5:31308549-31308568 MsG0580026143.01.T01:five_prime_UTR 25.0%
! GCTTAATGAATTGATCTATT+TGG + Chr5:31309108-31309127 MsG0580026143.01.T01:intron 25.0%
! GTTACATACAAAAAAAGATG+AGG + Chr5:31308761-31308780 MsG0580026143.01.T01:CDS 25.0%
! GTTACTTAATTTATCTGAGT+TGG + Chr5:31309334-31309353 MsG0580026143.01.T01:intron 25.0%
! GTTGAGTTTGATTATTGTAA+TGG + Chr5:31310969-31310988 MsG0580026143.01.T01:three_prime_UTR 25.0%
! TAAATTGTCTTGCTAAAACA+AGG - Chr5:31310056-31310075 None:intergenic 25.0%
! TATCTTAAGCTACATCAAAA+GGG + Chr5:31309404-31309423 MsG0580026143.01.T01:intron 25.0%
! TCTAAGTTTGATGTTGTTAA+AGG + Chr5:31308801-31308820 MsG0580026143.01.T01:CDS 25.0%
! TGACAAATGCTAAAATTGTT+AGG - Chr5:31309003-31309022 None:intergenic 25.0%
! TTATCTTAAGCTACATCAAA+AGG + Chr5:31309403-31309422 MsG0580026143.01.T01:intron 25.0%
!! AAGTTTCTTTTACACTAGAA+AGG + Chr5:31309503-31309522 MsG0580026143.01.T01:intron 25.0%
!! TGATTTTATATTGCCTAAAC+TGG + Chr5:31309068-31309087 MsG0580026143.01.T01:intron 25.0%
!!! AGTAGATATTTTTTACGGAT+TGG + Chr5:31308906-31308925 MsG0580026143.01.T01:intron 25.0%
!!! TCTGGTTTTTATGTTGTTTT+GGG + Chr5:31308717-31308736 MsG0580026143.01.T01:CDS 25.0%
!!! TTCTGGTTTTTATGTTGTTT+TGG + Chr5:31308716-31308735 MsG0580026143.01.T01:CDS 25.0%
!!! TTTTTTTTGCTGTTGCTATT+TGG + Chr5:31309298-31309317 MsG0580026143.01.T01:intron 25.0%
AAAACCTAAAACCAATGAGA+AGG - Chr5:31308411-31308430 None:intergenic 30.0%
AAACCTAAAACCAATGAGAA+GGG - Chr5:31308410-31308429 None:intergenic 30.0%
AACTATAACTAAACTGTGGT+TGG + Chr5:31309965-31309984 MsG0580026143.01.T01:intron 30.0%
AAGAACAACTCATAACCATT+AGG - Chr5:31309562-31309581 None:intergenic 30.0%
AATGAAGTTGTGATTGTATC+TGG + Chr5:31309934-31309953 MsG0580026143.01.T01:intron 30.0%
ACTATAACTAAACTGTGGTT+GGG + Chr5:31309966-31309985 MsG0580026143.01.T01:intron 30.0%
AGATGAGGATGAAAAGAATT+TGG + Chr5:31308776-31308795 MsG0580026143.01.T01:CDS 30.0%
ATGTAGCTTAAGATAAACAC+AGG - Chr5:31309399-31309418 None:intergenic 30.0%
ATTACTCATTCATCCTTGTA+AGG - Chr5:31310546-31310565 None:intergenic 30.0%
ATTGTGTATCTTTGTTACCA+CGG - Chr5:31310783-31310802 None:intergenic 30.0%
CAAACTCAACAACAATTCTT+TGG - Chr5:31310959-31310978 None:intergenic 30.0%
CACTGGTGATATTAAATCAA+CGG - Chr5:31309716-31309735 None:intergenic 30.0%
CCAATATTTGCATCAATTTC+AGG - Chr5:31308551-31308570 None:intergenic 30.0%
CCTGAAATTGATGCAAATAT+TGG + Chr5:31308548-31308567 MsG0580026143.01.T01:five_prime_UTR 30.0%
GATAGTATCAAAGATTGCAT+AGG - Chr5:31310864-31310883 None:intergenic 30.0%
TAGCATTTGTCAGTTTAGTT+AGG + Chr5:31309011-31309030 MsG0580026143.01.T01:intron 30.0%
TAGGACTGTTAGCTATTATA+TGG + Chr5:31310735-31310754 MsG0580026143.01.T01:three_prime_UTR 30.0%
TATTCTCATTGGTACTACTT+TGG - Chr5:31310026-31310045 None:intergenic 30.0%
! GTTTGTTTGCTGAAGATTAT+AGG + Chr5:31308739-31308758 MsG0580026143.01.T01:CDS 30.0%
! TATTACTGCAATGTTGGTAA+TGG + Chr5:31310074-31310093 MsG0580026143.01.T01:CDS 30.0%
! TCCTATTCTATTGAAGTGTT+CGG + Chr5:31310679-31310698 MsG0580026143.01.T01:three_prime_UTR 30.0%
! TCTTTTACACTAGAAAGGAA+AGG + Chr5:31309508-31309527 MsG0580026143.01.T01:intron 30.0%
!! AATGATGATGGATTTGGATT+TGG + Chr5:31308638-31308657 MsG0580026143.01.T01:exon 30.0%
!! ATGATGATGGATTTGGATTT+GGG + Chr5:31308639-31308658 MsG0580026143.01.T01:CDS 30.0%
!! ATGCAAATATTGGGTTTTGT+AGG + Chr5:31308558-31308577 MsG0580026143.01.T01:five_prime_UTR 30.0%
!! TCTCATTCTGTTTTCTAGAA+AGG + Chr5:31310922-31310941 MsG0580026143.01.T01:three_prime_UTR 30.0%
!!! TTGCTCCACTTTTAGTTTAT+TGG + Chr5:31308688-31308707 MsG0580026143.01.T01:CDS 30.0%
!!! TTGGGTTTTTGTTTTTGTGT+AGG + Chr5:31309984-31310003 MsG0580026143.01.T01:intron 30.0%
AACATATGGAATAGGTTTCC+AGG - Chr5:31310357-31310376 None:intergenic 35.0%
ACATATGGAATAGGTTTCCA+GGG - Chr5:31310356-31310375 None:intergenic 35.0%
AGACAAGTCAATGTTAGCAA+CGG - Chr5:31309618-31309637 None:intergenic 35.0%
ATATAACACCGTTGCTGAAT+TGG + Chr5:31309534-31309553 MsG0580026143.01.T01:intron 35.0%
ATTTGTGTGTGAGTTTCATG+TGG - Chr5:31308938-31308957 None:intergenic 35.0%
CGAACAAGAGGGAAAAATAT+CGG - Chr5:31308275-31308294 None:intergenic 35.0%
CTATACAATCATCCCATTGA+GGG + Chr5:31310204-31310223 MsG0580026143.01.T01:CDS 35.0%
CTCATTGTACCTTATTCGTT+TGG + Chr5:31310177-31310196 MsG0580026143.01.T01:CDS 35.0%
GAGAGTGAATGTGTTTGTAT+AGG - Chr5:31310146-31310165 None:intergenic 35.0%
GCATCTGTAGAAACTATAGT+GGG + Chr5:31309438-31309457 MsG0580026143.01.T01:intron 35.0%
GGTTAGGGTTAAAGATTAAG+TGG + Chr5:31308503-31308522 MsG0580026143.01.T01:five_prime_UTR 35.0%
GTTATGTGGGACAAAATCTT+GGG + Chr5:31310456-31310475 MsG0580026143.01.T01:CDS 35.0%
TAGACCTTGTAAACCTTACA+AGG + Chr5:31310530-31310549 MsG0580026143.01.T01:CDS 35.0%
TAGAGTTCAGAGCTAGATAT+GGG + Chr5:31309259-31309278 MsG0580026143.01.T01:intron 35.0%
TATAACACCGTTGCTGAATT+GGG + Chr5:31309535-31309554 MsG0580026143.01.T01:intron 35.0%
TATACAATCATCCCATTGAG+GGG + Chr5:31310205-31310224 MsG0580026143.01.T01:CDS 35.0%
TATAGAGCTCCAAACGAATA+AGG - Chr5:31310189-31310208 None:intergenic 35.0%
TCATCCTTGTAAGGTTTACA+AGG - Chr5:31310537-31310556 None:intergenic 35.0%
TCCGAACACTTCAATAGAAT+AGG - Chr5:31310683-31310702 None:intergenic 35.0%
TCTATACAATCATCCCATTG+AGG + Chr5:31310203-31310222 MsG0580026143.01.T01:CDS 35.0%
TGCATCTGTAGAAACTATAG+TGG + Chr5:31309437-31309456 MsG0580026143.01.T01:intron 35.0%
TGTTATGTGGGACAAAATCT+TGG + Chr5:31310455-31310474 MsG0580026143.01.T01:CDS 35.0%
TTAGAGTTCAGAGCTAGATA+TGG + Chr5:31309258-31309277 MsG0580026143.01.T01:intron 35.0%
TTCAATCATCCAAACCTTGA+AGG + Chr5:31308444-31308463 MsG0580026143.01.T01:five_prime_UTR 35.0%
TTGTCCCACATAACAAAGAA+CGG - Chr5:31310450-31310469 None:intergenic 35.0%
TTGTGTATCTTTGTTACCAC+GGG - Chr5:31310782-31310801 None:intergenic 35.0%
! GCTTTATCTTTTCTCCTATC+TGG + Chr5:31310252-31310271 MsG0580026143.01.T01:CDS 35.0%
!! AAGGAGAGAAGTATTCTCAT+TGG - Chr5:31310037-31310056 None:intergenic 35.0%
!! ATATTGCCTAAACTGGTTTG+TGG + Chr5:31309075-31309094 MsG0580026143.01.T01:intron 35.0%
!! GTGAGAAATGATGATGGATT+TGG + Chr5:31308632-31308651 MsG0580026143.01.T01:exon 35.0%
!! TGATGATGGATTTGGATTTG+GGG + Chr5:31308640-31308659 MsG0580026143.01.T01:CDS 35.0%
!!! ATTCCCTTCTCATTGGTTTT+AGG + Chr5:31308404-31308423 MsG0580026143.01.T01:five_prime_UTR 35.0%
!!! GATTAGGTTTTTTCCACAGA+AGG - Chr5:31308375-31308394 None:intergenic 35.0%
!!! TTTGTTTTTGTGTAGGTGAC+AGG + Chr5:31309991-31310010 MsG0580026143.01.T01:intron 35.0%
AAAAGATAAAGCTCCACCGA+TGG - Chr5:31310244-31310263 None:intergenic 40.0%
AAACCTCCACAAACCAGTTT+AGG - Chr5:31309084-31309103 None:intergenic 40.0%
AAGAAAAAGATGGATGCTCG+AGG - Chr5:31310285-31310304 None:intergenic 40.0%
ATCCAAACCTTGAAGGAACA+AGG + Chr5:31308451-31308470 MsG0580026143.01.T01:five_prime_UTR 40.0%
ATTAGGACCCAATTCAGCAA+CGG - Chr5:31309545-31309564 None:intergenic 40.0%
CTCTAATGAGTATGGCATGT+AGG - Chr5:31310484-31310503 None:intergenic 40.0%
GAAACTCACACACAAATGAG+AGG + Chr5:31308941-31308960 MsG0580026143.01.T01:intron 40.0%
GATGTACACCATCAACTCTA+CGG + Chr5:31310402-31310421 MsG0580026143.01.T01:CDS 40.0%
GCTCTCTTCTCTAATGAGTA+TGG - Chr5:31310492-31310511 None:intergenic 40.0%
GGCAAAGGAGAAGAAAAAGA+TGG - Chr5:31310295-31310314 None:intergenic 40.0%
GGGTTTCTGATGAGATGTTA+GGG + Chr5:31308661-31308680 MsG0580026143.01.T01:CDS 40.0%
GGGTTTGTGAGAAATGATGA+TGG + Chr5:31308626-31308645 MsG0580026143.01.T01:exon 40.0%
TAGCCACTATTTGACAACAC+TGG - Chr5:31309733-31309752 None:intergenic 40.0%
TATACAGGCATGATCACTCT+CGG - Chr5:31310642-31310661 None:intergenic 40.0%
TCACCAGTGTTGTCAAATAG+TGG + Chr5:31309727-31309746 MsG0580026143.01.T01:intron 40.0%
TCATTAGAGAAGAGAGCTAG+TGG + Chr5:31310495-31310514 MsG0580026143.01.T01:CDS 40.0%
TCCAAACCTTGAAGGAACAA+GGG + Chr5:31308452-31308471 MsG0580026143.01.T01:five_prime_UTR 40.0%
TCGGACAATCATGTGTGTAA+CGG - Chr5:31308256-31308275 None:intergenic 40.0%
TCTTCTCTTCTCTCTCTCTT+TGG + Chr5:31308340-31308359 MsG0580026143.01.T01:five_prime_UTR 40.0%
TCTTGAACGTGGGAAATAGA+GGG - Chr5:31309855-31309874 None:intergenic 40.0%
TTCGGGACTCAACAGATATT+CGG + Chr5:31308977-31308996 MsG0580026143.01.T01:intron 40.0%
TTGGCTCAAAAACGCTTCAT+AGG - Chr5:31309668-31309687 None:intergenic 40.0%
! GCAACCGTTCTTTGTTATGT+GGG + Chr5:31310443-31310462 MsG0580026143.01.T01:CDS 40.0%
! GCTCTGAACTCTAAACAACT+AGG - Chr5:31309252-31309271 None:intergenic 40.0%
!! CAATGTTGGTAATGGACACA+TGG + Chr5:31310082-31310101 MsG0580026143.01.T01:CDS 40.0%
!! GATGATGGATTTGGATTTGG+GGG + Chr5:31308641-31308660 MsG0580026143.01.T01:CDS 40.0%
!! TTGCTGTTGCTATTTGGCAT+AGG + Chr5:31309304-31309323 MsG0580026143.01.T01:intron 40.0%
!!! TAGGTTTTTTCCACAGAAGG+AGG - Chr5:31308372-31308391 None:intergenic 40.0%
!!! TTTTTTCCACAGAAGGAGGA+AGG - Chr5:31308368-31308387 None:intergenic 40.0%
CCAGAATCATGCGAACAAGA+GGG - Chr5:31308286-31308305 None:intergenic 45.0%
CGTTGTCAACTCTTGAACGT+GGG - Chr5:31309865-31309884 None:intergenic 45.0%
CTCTTGAACGTGGGAAATAG+AGG - Chr5:31309856-31309875 None:intergenic 45.0%
CTTTGCCACCATTAAAACCG+TGG + Chr5:31310308-31310327 MsG0580026143.01.T01:CDS 45.0%
GGGGTTTCTGATGAGATGTT+AGG + Chr5:31308660-31308679 MsG0580026143.01.T01:CDS 45.0%
GTTGCTGAATTGGGTCCTAA+TGG + Chr5:31309544-31309563 MsG0580026143.01.T01:intron 45.0%
TCAACAAGCTGTTCAAGCTG+TGG + Chr5:31308833-31308852 MsG0580026143.01.T01:CDS 45.0%
TCCAGAATCATGCGAACAAG+AGG - Chr5:31308287-31308306 None:intergenic 45.0%
TCCCTTGTTCCTTCAAGGTT+TGG - Chr5:31308456-31308475 None:intergenic 45.0%
TCGTTAAGAAGCCCCTCAAT+GGG - Chr5:31310219-31310238 None:intergenic 45.0%
TCTCTTCCCTTGTTCCTTCA+AGG - Chr5:31308461-31308480 None:intergenic 45.0%
TGTATCTTTGTTACCACGGG+CGG - Chr5:31310779-31310798 None:intergenic 45.0%
TTAGAGAAGAGAGCTAGTGG+CGG + Chr5:31310498-31310517 MsG0580026143.01.T01:CDS 45.0%
! ACTTGTTGCCGTAGAGTTGA+TGG - Chr5:31310413-31310432 None:intergenic 45.0%
! CGCAACCGTTCTTTGTTATG+TGG + Chr5:31310442-31310461 MsG0580026143.01.T01:CDS 45.0%
!! AGAGTTGATGGTGTACATCG+TGG - Chr5:31310401-31310420 None:intergenic 45.0%
!! CACGGTTTTAATGGTGGCAA+AGG - Chr5:31310310-31310329 None:intergenic 45.0%
!! GTCATCCACGGTTTTAATGG+TGG - Chr5:31310316-31310335 None:intergenic 45.0%
!! GTGGTCATCCACGGTTTTAA+TGG - Chr5:31310319-31310338 None:intergenic 45.0%
!! TTGCCTAAACTGGTTTGTGG+AGG + Chr5:31309078-31309097 MsG0580026143.01.T01:intron 45.0%
AAAACCGTGGATGACCACTG+TGG + Chr5:31310321-31310340 MsG0580026143.01.T01:CDS 50.0%
AAACCGTGGATGACCACTGT+GGG + Chr5:31310322-31310341 MsG0580026143.01.T01:CDS 50.0%
AACAGTCCTAGCTTCCATGC+CGG - Chr5:31310725-31310744 None:intergenic 50.0%
ACACACAAATGAGAGGTCCG+AGG + Chr5:31308948-31308967 MsG0580026143.01.T01:intron 50.0%
AGAGGTCCGAGGTTCGAATT+CGG + Chr5:31308959-31308978 MsG0580026143.01.T01:intron 50.0%
CCCTCTTGTTCGCATGATTC+TGG + Chr5:31308283-31308302 MsG0580026143.01.T01:five_prime_UTR 50.0%
CTCGAGGACTCATACCAGAT+AGG - Chr5:31310269-31310288 None:intergenic 50.0%
CTTCTTAACGACACCATCGG+TGG + Chr5:31310228-31310247 MsG0580026143.01.T01:CDS 50.0%
GCGTTGTCAACTCTTGAACG+TGG - Chr5:31309866-31309885 None:intergenic 50.0%
GGGCTTCTTAACGACACCAT+CGG + Chr5:31310225-31310244 MsG0580026143.01.T01:CDS 50.0%
GGTGCTCGTCATCATAACGT+GGG - Chr5:31310621-31310640 None:intergenic 50.0%
GTATCTTTGTTACCACGGGC+GGG - Chr5:31310778-31310797 None:intergenic 50.0%
GTCGTTAAGAAGCCCCTCAA+TGG - Chr5:31310220-31310239 None:intergenic 50.0%
TAACCCACAGTGGTCATCCA+CGG - Chr5:31310328-31310347 None:intergenic 50.0%
TGAGTCCCGAATTCGAACCT+CGG - Chr5:31308968-31308987 None:intergenic 50.0%
TTGGAACCTTCCTCCTTCTG+TGG + Chr5:31308359-31308378 MsG0580026143.01.T01:five_prime_UTR 50.0%
! TGGATGACCACTGTGGGTTA+TGG + Chr5:31310328-31310347 MsG0580026143.01.T01:CDS 50.0%
ACCACGTCGAAACCACTCTC+CGG + Chr5:31310703-31310722 MsG0580026143.01.T01:three_prime_UTR 55.0%
CGGTGCTCGTCATCATAACG+TGG - Chr5:31310622-31310641 None:intergenic 55.0%
GAGGTCCGAGGTTCGAATTC+GGG + Chr5:31308960-31308979 MsG0580026143.01.T01:intron 55.0%
CACTCTCCGGCATGGAAGCT+AGG + Chr5:31310716-31310735 MsG0580026143.01.T01:three_prime_UTR 60.0%
CAGGGAGCCATAACCCACAG+TGG - Chr5:31310338-31310357 None:intergenic 60.0%
CGTTTGATGTATCCCGCCCG+TGG + Chr5:31310763-31310782 MsG0580026143.01.T01:three_prime_UTR 60.0%
CTAGCTTCCATGCCGGAGAG+TGG - Chr5:31310718-31310737 None:intergenic 60.0%
GTCGAAACCACTCTCCGGCA+TGG + Chr5:31310708-31310727 MsG0580026143.01.T01:three_prime_UTR 60.0%
! CACTGTGGGTTATGGCTCCC+TGG + Chr5:31310336-31310355 MsG0580026143.01.T01:CDS 60.0%
GCCGGAGAGTGGTTTCGACG+TGG - Chr5:31310707-31310726 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 31308233 31311093 31308233 ID=MsG0580026143.01;Name=MsG0580026143.01
Chr5 mRNA 31308233 31311093 31308233 ID=MsG0580026143.01.T01;Parent=MsG0580026143.01;Name=MsG0580026143.01.T01;_AED=0.36;_eAED=0.36;_QI=406|1|1|1|1|1|2|533|263
Chr5 exon 31308233 31308875 31308233 ID=MsG0580026143.01.T01:exon:13051;Parent=MsG0580026143.01.T01
Chr5 exon 31310006 31311093 31310006 ID=MsG0580026143.01.T01:exon:13052;Parent=MsG0580026143.01.T01
Chr5 five_prime_UTR 31308233 31308638 31308233 ID=MsG0580026143.01.T01:five_prime_utr;Parent=MsG0580026143.01.T01
Chr5 CDS 31308639 31308875 31308639 ID=MsG0580026143.01.T01:cds;Parent=MsG0580026143.01.T01
Chr5 CDS 31310006 31310560 31310006 ID=MsG0580026143.01.T01:cds;Parent=MsG0580026143.01.T01
Chr5 three_prime_UTR 31310561 31311093 31310561 ID=MsG0580026143.01.T01:three_prime_utr;Parent=MsG0580026143.01.T01
Gene Sequence

>MsG0580026143.01.T01

ATGATGATGGATTTGGATTTGGGGGTTTCTGATGAGATGTTAGGGACTTTTGCTCCACTTTTAGTTTATTGGATTTATTCTGGTTTTTATGTTGTTTTGGGTTTGTTTGCTGAAGATTATAGGTTACATACAAAAAAAGATGAGGATGAAAAGAATTTGGTTTCTAAGTTTGATGTTGTTAAAGGTGTTCTTCTTCAACAAGCTGTTCAAGCTGTGGTTGCAACCCTTTTGTTTGCAGTGACAGGAAGTGATAGCCAAAGTAGTACCAATGAGAATACTTCTCTCCTTGTTTTAGCAAGACAATTTATTACTGCAATGTTGGTAATGGACACATGGCAATACTTTATGCACAGATACATGCATCAGAACAAGTTCCTATACAAACACATTCACTCTCTACATCACAGACTCATTGTACCTTATTCGTTTGGAGCTCTATACAATCATCCCATTGAGGGGCTTCTTAACGACACCATCGGTGGAGCTTTATCTTTTCTCCTATCTGGTATGAGTCCTCGAGCATCCATCTTTTTCTTCTCCTTTGCCACCATTAAAACCGTGGATGACCACTGTGGGTTATGGCTCCCTGGAAACCTATTCCATATGTTTTTCAATAACAATTCTGCTTACCACGATGTACACCATCAACTCTACGGCAACAAGTACAACTTCTCGCAACCGTTCTTTGTTATGTGGGACAAAATCTTGGGTACCTACATGCCATACTCATTAGAGAAGAGAGCTAGTGGCGGTTTTGAATCTAGACCTTGTAAACCTTACAAGGATGAATGA
Protein sequence

>MsG0580026143.01.T01

MMMDLDLGVSDEMLGTFAPLLVYWIYSGFYVVLGLFAEDYRLHTKKDEDEKNLVSKFDVVKGVLLQQAVQAVVATLLFAVTGSDSQSSTNENTSLLVLARQFITAMLVMDTWQYFMHRYMHQNKFLYKHIHSLHHRLIVPYSFGALYNHPIEGLLNDTIGGALSFLLSGMSPRASIFFFSFATIKTVDDHCGLWLPGNLFHMFFNNNSAYHDVHHQLYGNKYNFSQPFFVMWDKILGTYMPYSLEKRASGGFESRPCKPYKDE*