AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026281.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026281.01.T01 MTR_5g043190 97.778 180 4 0 1 180 1 180 7.03e-128 357
MsG0580026281.01.T01 MTR_4g109140 63.536 181 58 2 1 180 1 174 3.14e-80 236
MsG0580026281.01.T01 MTR_7g070000 50.000 180 80 2 1 180 1 170 1.73e-59 183
MsG0580026281.01.T01 MTR_4g014490 51.205 166 78 1 15 180 8 170 1.73e-59 183
MsG0580026281.01.T01 MTR_7g108775 39.759 83 48 2 63 144 33 114 5.49e-14 66.6
MsG0580026281.01.T01 MTR_7g108775 39.759 83 48 2 63 144 33 114 6.39e-14 67.0
MsG0580026281.01.T01 MTR_8g085590 32.432 111 71 4 63 171 33 141 1.47e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026281.01.T01 AT2G03350 75.141 177 42 1 1 177 1 175 2.67e-95 274
MsG0580026281.01.T01 AT5G37070 48.889 180 82 2 1 180 1 170 8.50e-61 186
MsG0580026281.01.T01 AT3G08890 48.333 180 83 2 1 180 1 170 3.60e-59 182
MsG0580026281.01.T01 AT3G08890 48.333 180 83 2 1 180 1 170 3.60e-59 182
MsG0580026281.01.T01 AT5G01610 49.398 166 81 1 15 180 8 170 2.02e-58 181
MsG0580026281.01.T01 AT5G01610 49.398 166 81 1 15 180 8 170 2.02e-58 181
MsG0580026281.01.T01 AT4G02370 29.703 101 67 2 64 163 35 132 3.54e-13 64.7
MsG0580026281.01.T01 AT1G02816 25.893 112 77 3 64 172 35 143 5.60e-13 63.9
MsG0580026281.01.T01 AT5G16380 26.562 128 90 3 49 174 19 144 2.10e-12 63.2

Find 57 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACACAAGAACGAGAAAA+AGG 0.223448 5:+33919414 None:intergenic
ACACTGAATGGAAGTTAATT+TGG 0.226830 5:-33916128 MsG0580026281.01.T01:three_prime_UTR
CTTCTCTATATTATATATAT+TGG 0.242497 5:-33916180 MsG0580026281.01.T01:three_prime_UTR
GTTACAAATCTGATAAGATT+TGG 0.265998 5:-33916382 MsG0580026281.01.T01:CDS
GTTGGATATGTTTACATAGA+AGG 0.294741 5:-33916221 MsG0580026281.01.T01:three_prime_UTR
TATTGGATGTACTGCAGTTC+TGG 0.333625 5:-33916163 MsG0580026281.01.T01:three_prime_UTR
GGGAAAGCTTTCACAAATAG+AGG 0.335889 5:-33916459 MsG0580026281.01.T01:CDS
TGAAAACAAAAGTTCTTGTA+TGG 0.338172 5:-33916433 MsG0580026281.01.T01:CDS
TTACAAATCTGATAAGATTT+GGG 0.365699 5:-33916381 MsG0580026281.01.T01:CDS
AAAAGTTCTTGTATGGGTTA+AGG 0.386610 5:-33916426 MsG0580026281.01.T01:CDS
TCATTTATTGTCACACTGAA+TGG 0.387051 5:-33916140 MsG0580026281.01.T01:three_prime_UTR
AGATTGGACTTGGTGATTCA+TGG 0.388734 5:-33919311 MsG0580026281.01.T01:CDS
CTCAAGCACTGTTGAAGAAA+AGG 0.392322 5:-33917677 MsG0580026281.01.T01:CDS
ACATAGAAGGTGTAGGGGTT+AGG 0.392744 5:-33916208 MsG0580026281.01.T01:three_prime_UTR
TTATAATCTTTGAGGAGATC+TGG 0.426726 5:+33916638 None:intergenic
CTGTTGAAGAAAAGGCCAAA+TGG 0.427835 5:-33917669 MsG0580026281.01.T01:CDS
TAGTTTGTTGAAAACCCATT+TGG 0.430521 5:+33917654 None:intergenic
GTATAGTTAGTCTAATTTGT+TGG 0.440191 5:-33916239 MsG0580026281.01.T01:three_prime_UTR
TGTTGAAGAAAAGGCCAAAT+GGG 0.457433 5:-33917668 MsG0580026281.01.T01:CDS
GAGTCAAAGGTGTTCTTACA+AGG 0.462275 5:-33916481 MsG0580026281.01.T01:CDS
GGTGTATCGTAAGCATCCTT+TGG 0.469228 5:+33916332 None:intergenic
TGCATGTGAGGTCAGCTTCA+AGG 0.469233 5:-33916531 MsG0580026281.01.T01:CDS
ATGTTTACATAGAAGGTGTA+GGG 0.472196 5:-33916214 MsG0580026281.01.T01:three_prime_UTR
TGTAAGAACACCTTTGACTC+GGG 0.474227 5:+33916484 None:intergenic
TGTTTGTCTTGTTGTCTAGC+TGG 0.488940 5:+33919491 None:intergenic
TCGTTGTCGTTCTATTCGTA+TGG 0.496045 5:-33916261 MsG0580026281.01.T01:three_prime_UTR
AAGATTATAATTTACCGCTC+GGG 0.497684 5:-33916625 MsG0580026281.01.T01:CDS
GTAGACAATCAGCTTACCCT+TGG 0.505031 5:+33916562 None:intergenic
CCCACAGGTAAGCCACAGAA+AGG 0.506420 5:-33916665 MsG0580026281.01.T01:intron
AAAGATTATAATTTACCGCT+CGG 0.506636 5:-33916626 MsG0580026281.01.T01:CDS
AAAGTGGCAAGCTTGAAGAT+TGG 0.509572 5:-33919327 MsG0580026281.01.T01:CDS
TGATACTCTAATAGCATCAC+GGG 0.514685 5:+33916310 None:intergenic
GATAAGATTTGGGTTACTGC+TGG 0.516200 5:-33916371 MsG0580026281.01.T01:CDS
GCGGTAAATTATAATCTTTG+AGG 0.520183 5:+33916630 None:intergenic
GAAAACAAAAGTTCTTGTAT+GGG 0.524444 5:-33916432 MsG0580026281.01.T01:CDS
TATGTTTACATAGAAGGTGT+AGG 0.527245 5:-33916215 MsG0580026281.01.T01:three_prime_UTR
TTGTAAGAACACCTTTGACT+CGG 0.560711 5:+33916483 None:intergenic
CTGATACTCTAATAGCATCA+CGG 0.563344 5:+33916309 None:intergenic
CGATATGCAACCCGAGTCAA+AGG 0.564195 5:-33916494 MsG0580026281.01.T01:CDS
GCAAGCTTGAAGATTGGACT+TGG 0.564615 5:-33919321 MsG0580026281.01.T01:CDS
TTGCAACTAACAACACAAGA+TGG 0.569675 5:-33919364 MsG0580026281.01.T01:exon
GGAGAAAGCTCTCACTAAAG+TGG 0.571492 5:-33919343 MsG0580026281.01.T01:CDS
CTACTTACCATCTGCATGTG+AGG 0.574237 5:-33916543 MsG0580026281.01.T01:CDS
CTGCTGGTGTTAAGAAGTCA+AGG 0.578489 5:-33916355 MsG0580026281.01.T01:CDS
GTGACAAGTGTGACAGTAGA+GGG 0.581696 5:-33916404 MsG0580026281.01.T01:CDS
AGATCTGGTAAACCTTTCTG+TGG 0.597603 5:+33916653 None:intergenic
GTCAAAGGTGTTCTTACAAG+GGG 0.598086 5:-33916479 MsG0580026281.01.T01:CDS
GGAAAGCTTTCACAAATAGA+GGG 0.603339 5:-33916458 MsG0580026281.01.T01:CDS
AAGCTGACCTCACATGCAGA+TGG 0.613619 5:+33916536 None:intergenic
GGTGACAAGTGTGACAGTAG+AGG 0.615714 5:-33916405 MsG0580026281.01.T01:CDS
TATGAGTTTGACGAAACCAA+GGG 0.622312 5:-33916578 MsG0580026281.01.T01:CDS
TTATGAGTTTGACGAAACCA+AGG 0.622560 5:-33916579 MsG0580026281.01.T01:CDS
TGTTTACATAGAAGGTGTAG+GGG 0.639448 5:-33916213 MsG0580026281.01.T01:three_prime_UTR
TGTTAAGAAGTCAAGGCCAA+AGG 0.647193 5:-33916348 MsG0580026281.01.T01:CDS
AGTCAAAGGTGTTCTTACAA+GGG 0.654060 5:-33916480 MsG0580026281.01.T01:CDS
GGTTGCATATCGCAGTACAG+AGG 0.698294 5:+33916505 None:intergenic
GATACTCTAATAGCATCACG+GGG 0.785323 5:+33916311 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTCTCTATATTATATATAT+TGG - Chr5:33919429-33919448 MsG0580026281.01.T01:five_prime_UTR 15.0%
!! TAATAAATTAATCACATGAT+CGG + Chr5:33917497-33917516 None:intergenic 15.0%
!! TGAGTTTAAATTTAAATATC+AGG - Chr5:33918389-33918408 MsG0580026281.01.T01:intron 15.0%
!! TTCTCAATATTACTAATAAA+TGG + Chr5:33917776-33917795 None:intergenic 15.0%
!!! ATACATTTTATTATGCTTTA+TGG + Chr5:33917815-33917834 None:intergenic 15.0%
!!! TAATTTATTTATTTTTGTCC+TGG - Chr5:33917352-33917371 MsG0580026281.01.T01:intron 15.0%
!!! TTGTTATTTCATAAATTAGA+GGG + Chr5:33918463-33918482 None:intergenic 15.0%
!!! TTTGTTATTTCATAAATTAG+AGG + Chr5:33918464-33918483 None:intergenic 15.0%
!!! TTTTGGGTATATTAAAAAAA+TGG - Chr5:33917204-33917223 MsG0580026281.01.T01:intron 15.0%
!! ATTGATGTTCCTTAATTTAA+GGG + Chr5:33918492-33918511 None:intergenic 20.0%
!! TTAAAATCAATGAAAAAACC+TGG - Chr5:33918554-33918573 MsG0580026281.01.T01:intron 20.0%
!! TTACAAATCTGATAAGATTT+GGG - Chr5:33919228-33919247 MsG0580026281.01.T01:intron 20.0%
!!! ATTTTGCAAAACAATTCATT+CGG - Chr5:33916990-33917009 MsG0580026281.01.T01:intron 20.0%
!!! CAAACATTTTTCATATACAA+AGG - Chr5:33916135-33916154 MsG0580026281.01.T01:three_prime_UTR 20.0%
!!! GACCAAAAAATAGTTTTTTT+GGG - Chr5:33917188-33917207 MsG0580026281.01.T01:intron 20.0%
!!! TACCCAAAAAAACTATTTTT+TGG + Chr5:33917193-33917212 None:intergenic 20.0%
!!! TATTAAAAAAATGGCCTTTT+TGG - Chr5:33917213-33917232 MsG0580026281.01.T01:intron 20.0%
!!! TGACCAAAAAATAGTTTTTT+TGG - Chr5:33917187-33917206 MsG0580026281.01.T01:intron 20.0%
!!! TTTGATGTTTCTTTAGTTTA+GGG - Chr5:33917112-33917131 MsG0580026281.01.T01:intron 20.0%
!!! TTTTATTATGCTTTATGGTT+TGG + Chr5:33917810-33917829 None:intergenic 20.0%
!!! TTTTGATGTTTCTTTAGTTT+AGG - Chr5:33917111-33917130 MsG0580026281.01.T01:intron 20.0%
! AAAGATTATAATTTACCGCT+CGG - Chr5:33918983-33919002 MsG0580026281.01.T01:intron 25.0%
! AATTTCATTGAGAGATTACA+AGG - Chr5:33917412-33917431 MsG0580026281.01.T01:intron 25.0%
! ATTTCATTGAGAGATTACAA+GGG - Chr5:33917413-33917432 MsG0580026281.01.T01:intron 25.0%
! GAAAACAAAAGTTCTTGTAT+GGG - Chr5:33919177-33919196 MsG0580026281.01.T01:intron 25.0%
! GATTATTCAAATCAATATCC+AGG + Chr5:33918575-33918594 None:intergenic 25.0%
! GATTGATGTTCCTTAATTTA+AGG + Chr5:33918493-33918512 None:intergenic 25.0%
! GCTAAATATAAATTGATCCA+AGG + Chr5:33916951-33916970 None:intergenic 25.0%
! GTTACAAATCTGATAAGATT+TGG - Chr5:33919227-33919246 MsG0580026281.01.T01:intron 25.0%
! TATTCCTCAAAGATTTGAAT+AGG - Chr5:33916609-33916628 MsG0580026281.01.T01:CDS 25.0%
! TGAAAACAAAAGTTCTTGTA+TGG - Chr5:33919176-33919195 MsG0580026281.01.T01:intron 25.0%
! TTCCATAAATGAATATGCAA+TGG - Chr5:33917143-33917162 MsG0580026281.01.T01:intron 25.0%
! TTGATTGTAGTGCTAAAATT+TGG - Chr5:33918626-33918645 MsG0580026281.01.T01:intron 25.0%
! TTTCAACAAACTAAAAGGTA+TGG - Chr5:33917965-33917984 MsG0580026281.01.T01:intron 25.0%
!! AAAATTGATTCCTCTGAAAA+TGG + Chr5:33916656-33916675 None:intergenic 25.0%
!! AAATTGATTTCGACTTAAAG+TGG + Chr5:33916974-33916993 None:intergenic 25.0%
!! AATTGATTTCGACTTAAAGT+GGG + Chr5:33916973-33916992 None:intergenic 25.0%
!! GTATAGTTAGTCTAATTTGT+TGG - Chr5:33919370-33919389 MsG0580026281.01.T01:five_prime_UTR 25.0%
!! TCATAACAGATTATGTTTTG+AGG + Chr5:33919016-33919035 None:intergenic 25.0%
!! TTTTTACCTTTCTAAGTCAT+AGG - Chr5:33917304-33917323 MsG0580026281.01.T01:intron 25.0%
!!! ACAATGTTTTTGCTGATATA+AGG - Chr5:33917889-33917908 MsG0580026281.01.T01:intron 25.0%
!!! ATTTTAGCACTACAATCAAA+AGG + Chr5:33918625-33918644 None:intergenic 25.0%
!!! TCCATGATGTTATTTCTAAA+TGG - Chr5:33917529-33917548 MsG0580026281.01.T01:intron 25.0%
AAACAAGCTTAGTATGTGTT+TGG + Chr5:33917039-33917058 None:intergenic 30.0%
AAGATTATAATTTACCGCTC+GGG - Chr5:33918984-33919003 MsG0580026281.01.T01:intron 30.0%
ACACTGAATGGAAGTTAATT+TGG - Chr5:33919481-33919500 MsG0580026281.01.T01:five_prime_UTR 30.0%
AGCAGTCTATTTGAATCAAT+TGG + Chr5:33918051-33918070 None:intergenic 30.0%
ATCTCCTATTCAAATCTTTG+AGG + Chr5:33916616-33916635 None:intergenic 30.0%
CAAATCAGTCCCTTAAATTA+AGG - Chr5:33918480-33918499 MsG0580026281.01.T01:intron 30.0%
CATGAAAAACTTATTATCGC+AGG + Chr5:33918159-33918178 None:intergenic 30.0%
CTAGGCAGTTATTATTTCAA+TGG - Chr5:33918848-33918867 MsG0580026281.01.T01:intron 30.0%
CTTGATTGATGCAAAATTCT+TGG + Chr5:33917628-33917647 None:intergenic 30.0%
GAAAAAATGCATAAACTCAC+TGG + Chr5:33918013-33918032 None:intergenic 30.0%
GCCATTTAGAAATAACATCA+TGG + Chr5:33917533-33917552 None:intergenic 30.0%
GCGGTAAATTATAATCTTTG+AGG + Chr5:33918982-33919001 None:intergenic 30.0%
GTCCATTGCATATTCATTTA+TGG + Chr5:33917148-33917167 None:intergenic 30.0%
GTTGGATATGTTTACATAGA+AGG - Chr5:33919388-33919407 MsG0580026281.01.T01:five_prime_UTR 30.0%
TATGATCTTCAAATTTGCGA+GGG + Chr5:33918733-33918752 None:intergenic 30.0%
TCATTTATTGTCACACTGAA+TGG - Chr5:33919469-33919488 MsG0580026281.01.T01:five_prime_UTR 30.0%
TGATTCCTCTAAACCAATTT+GGG + Chr5:33916793-33916812 None:intergenic 30.0%
TTAGTATGTGTTTGGTTATG+CGG + Chr5:33917031-33917050 None:intergenic 30.0%
TTATGATCTTCAAATTTGCG+AGG + Chr5:33918734-33918753 None:intergenic 30.0%
TTGATTCCTCTAAACCAATT+TGG + Chr5:33916794-33916813 None:intergenic 30.0%
! AAAAGTTCTTGTATGGGTTA+AGG - Chr5:33919183-33919202 MsG0580026281.01.T01:intron 30.0%
! AATCAACCTTGGATTAGAAT+TGG - Chr5:33916549-33916568 MsG0580026281.01.T01:CDS 30.0%
! AGAGGAATCAAATTGATTCT+TGG - Chr5:33916803-33916822 MsG0580026281.01.T01:intron 30.0%
! ATGTTTACATAGAAGGTGTA+GGG - Chr5:33919395-33919414 MsG0580026281.01.T01:five_prime_UTR 30.0%
! TATGTTTACATAGAAGGTGT+AGG - Chr5:33919394-33919413 MsG0580026281.01.T01:five_prime_UTR 30.0%
! TCTGAAAGATCCAAATTTTC+AGG + Chr5:33917257-33917276 None:intergenic 30.0%
! TGGGTTTTCAACAAACTAAA+AGG - Chr5:33917960-33917979 MsG0580026281.01.T01:intron 30.0%
! TTATAATCTTTGAGGAGATC+TGG + Chr5:33918974-33918993 None:intergenic 30.0%
!! AATGAATGAAGTCATTTTCC+AGG + Chr5:33917373-33917392 None:intergenic 30.0%
!! ATTGTTTGTTTTCAAACTGC+AGG + Chr5:33917449-33917468 None:intergenic 30.0%
!! CTTGGTCTTTGAAATTGTAA+GGG - Chr5:33918423-33918442 MsG0580026281.01.T01:intron 30.0%
!! TAGTTTGTTGAAAACCCATT+TGG + Chr5:33917958-33917977 None:intergenic 30.0%
!! TCTTGGTCTTTGAAATTGTA+AGG - Chr5:33918422-33918441 MsG0580026281.01.T01:intron 30.0%
!! TTGTTTGTTTTCAAACTGCA+GGG + Chr5:33917448-33917467 None:intergenic 30.0%
!!! ACATACTAAGCTTGTTTTAG+TGG - Chr5:33917042-33917061 MsG0580026281.01.T01:intron 30.0%
!!! CTACAATTGATTTTTAGCCT+TGG - Chr5:33916931-33916950 MsG0580026281.01.T01:intron 30.0%
!!! GTTCTTTTTTCCCTTTAATG+TGG - Chr5:33916437-33916456 MsG0580026281.01.T01:CDS 30.0%
AAGCTACATATGACATGTAG+AGG + Chr5:33917562-33917581 None:intergenic 35.0%
AATGGAAACTTCCCATGAAA+AGG + Chr5:33916486-33916505 None:intergenic 35.0%
ACAACACAAGAACGAGAAAA+AGG + Chr5:33916198-33916217 None:intergenic 35.0%
ACCCAAAAAAGCCACATTAA+AGG + Chr5:33916451-33916470 None:intergenic 35.0%
ACGAAAACCATTCGTTTGAA+TGG - Chr5:33917843-33917862 MsG0580026281.01.T01:intron 35.0%
AGTAACACTAACACCAAACA+GGG + Chr5:33917869-33917888 None:intergenic 35.0%
AGTCACATCTCTGTAACTTA+TGG + Chr5:33918799-33918818 None:intergenic 35.0%
ATAAACTCACTGGAACATCT+AGG + Chr5:33918003-33918022 None:intergenic 35.0%
ATAAGTTACAGAGATGTGAC+TGG - Chr5:33918798-33918817 MsG0580026281.01.T01:intron 35.0%
ATATGACATGTAGAGGATCA+TGG + Chr5:33917555-33917574 None:intergenic 35.0%
ATGGAAACTTCCCATGAAAA+GGG + Chr5:33916485-33916504 None:intergenic 35.0%
ATGTGTTGTCTACTACTCTA+TGG - Chr5:33917728-33917747 MsG0580026281.01.T01:intron 35.0%
CAACACAAGAACGAGAAAAA+GGG + Chr5:33916197-33916216 None:intergenic 35.0%
CAAGTACCAATTCTAATCCA+AGG + Chr5:33916558-33916577 None:intergenic 35.0%
CCCAAAAAAGCCACATTAAA+GGG + Chr5:33916450-33916469 None:intergenic 35.0%
CTAAATTAGCCCACCTAAAT+TGG - Chr5:33916693-33916712 MsG0580026281.01.T01:intron 35.0%
CTGATACTCTAATAGCATCA+CGG + Chr5:33919303-33919322 None:intergenic 35.0%
GGAAAGCTTTCACAAATAGA+GGG - Chr5:33919151-33919170 MsG0580026281.01.T01:intron 35.0%
GTTGAATGGACCTGAAAATT+TGG - Chr5:33917244-33917263 MsG0580026281.01.T01:intron 35.0%
GTTGTTCCTATGACTTAGAA+AGG + Chr5:33917313-33917332 None:intergenic 35.0%
TAAACTCACTGGAACATCTA+GGG + Chr5:33918002-33918021 None:intergenic 35.0%
TAAATGGATGATGACTCACT+TGG + Chr5:33917760-33917779 None:intergenic 35.0%
TAGATCTCTCAAATCCCAAA+TGG + Chr5:33916504-33916523 None:intergenic 35.0%
TAGGAGATTAAGTGTGTGTT+TGG - Chr5:33916628-33916647 MsG0580026281.01.T01:CDS 35.0%
TAGTAACACTAACACCAAAC+AGG + Chr5:33917870-33917889 None:intergenic 35.0%
TATCAGGTTAATGAGCATCT+TGG - Chr5:33918405-33918424 MsG0580026281.01.T01:intron 35.0%
TATGAGTTTGACGAAACCAA+GGG - Chr5:33919031-33919050 MsG0580026281.01.T01:intron 35.0%
TGATACTCTAATAGCATCAC+GGG + Chr5:33919302-33919321 None:intergenic 35.0%
TGTTGAAGAAAAGGCCAAAT+GGG - Chr5:33917941-33917960 MsG0580026281.01.T01:intron 35.0%
TTATGAGTTTGACGAAACCA+AGG - Chr5:33919030-33919049 MsG0580026281.01.T01:intron 35.0%
TTCAACACAGACAACCAAAA+AGG + Chr5:33917230-33917249 None:intergenic 35.0%
TTCATGGGAAGTTTCCATTT+GGG - Chr5:33916487-33916506 MsG0580026281.01.T01:CDS 35.0%
TTGCAACTAACAACACAAGA+TGG - Chr5:33916245-33916264 MsG0580026281.01.T01:three_prime_UTR 35.0%
TTGCTTGTTTGAATCAACCT+TGG - Chr5:33916538-33916557 MsG0580026281.01.T01:CDS 35.0%
TTGTAAGAACACCTTTGACT+CGG + Chr5:33919129-33919148 None:intergenic 35.0%
TTTCACCTAGTTTAGCATGA+CGG + Chr5:33918079-33918098 None:intergenic 35.0%
TTTCATGGGAAGTTTCCATT+TGG - Chr5:33916486-33916505 MsG0580026281.01.T01:CDS 35.0%
! CATATAAGAGAAAGTGTGTC+AGG + Chr5:33918214-33918233 None:intergenic 35.0%
! CTTGGATTAGAATTGGTACT+TGG - Chr5:33916556-33916575 MsG0580026281.01.T01:CDS 35.0%
! TCTCATGGAATGTTCTGTTT+TGG - Chr5:33917066-33917085 MsG0580026281.01.T01:intron 35.0%
! TGTTTACATAGAAGGTGTAG+GGG - Chr5:33919396-33919415 MsG0580026281.01.T01:five_prime_UTR 35.0%
!! AGTCAAAGGTGTTCTTACAA+GGG - Chr5:33919129-33919148 MsG0580026281.01.T01:intron 35.0%
!! GTTAGTTTGACCCTTTTCAT+GGG - Chr5:33916472-33916491 MsG0580026281.01.T01:CDS 35.0%
!! TGTTAGTTTGACCCTTTTCA+TGG - Chr5:33916471-33916490 MsG0580026281.01.T01:CDS 35.0%
!! TGTTTGTTTTCAAACTGCAG+GGG + Chr5:33917447-33917466 None:intergenic 35.0%
!! TTTTGGTTGTCTGTGTTGAA+TGG - Chr5:33917230-33917249 MsG0580026281.01.T01:intron 35.0%
!!! CCCTTTAATGTGGCTTTTTT+GGG - Chr5:33916447-33916466 MsG0580026281.01.T01:CDS 35.0%
!!! TCCCTTTAATGTGGCTTTTT+TGG - Chr5:33916446-33916465 MsG0580026281.01.T01:CDS 35.0%
AAAAGGGTGAAGTGAGAATG+AGG + Chr5:33918191-33918210 None:intergenic 40.0%
AAAGTGGCAAGCTTGAAGAT+TGG - Chr5:33916282-33916301 MsG0580026281.01.T01:exon 40.0%
ACAATGTGAAGAAACCAGCT+GGG + Chr5:33918323-33918342 None:intergenic 40.0%
AGATCTGGTAAACCTTTCTG+TGG + Chr5:33918959-33918978 None:intergenic 40.0%
CTGTTGAAGAAAAGGCCAAA+TGG - Chr5:33917940-33917959 MsG0580026281.01.T01:intron 40.0%
GAATCAAACTTGAGACATCG+AGG - Chr5:33916725-33916744 MsG0580026281.01.T01:intron 40.0%
GATACTCTAATAGCATCACG+GGG + Chr5:33919301-33919320 None:intergenic 40.0%
GGGAAAGCTTTCACAAATAG+AGG - Chr5:33919150-33919169 MsG0580026281.01.T01:intron 40.0%
GTCTACACGCAAAAATGACT+AGG - Chr5:33918830-33918849 MsG0580026281.01.T01:intron 40.0%
GTCTCAAGTTTGATTCTCTC+CGG + Chr5:33916721-33916740 None:intergenic 40.0%
TATTGGATGTACTGCAGTTC+TGG - Chr5:33919446-33919465 MsG0580026281.01.T01:five_prime_UTR 40.0%
TCCTCTAAACCAATTTGGGT+TGG + Chr5:33916789-33916808 None:intergenic 40.0%
TCGTTGTCGTTCTATTCGTA+TGG - Chr5:33919348-33919367 MsG0580026281.01.T01:CDS 40.0%
TGTAAGAACACCTTTGACTC+GGG + Chr5:33919128-33919147 None:intergenic 40.0%
TGTTAAGAAGTCAAGGCCAA+AGG - Chr5:33919261-33919280 MsG0580026281.01.T01:CDS 40.0%
TTAGAGAAGAGTGAACTCAG+AGG + Chr5:33918299-33918318 None:intergenic 40.0%
! AAGTACCGTCATGCTAAACT+AGG - Chr5:33918071-33918090 MsG0580026281.01.T01:intron 40.0%
! AGAGAAAGTGTGTCAGGAAA+AGG + Chr5:33918208-33918227 None:intergenic 40.0%
! CTCAAGCACTGTTGAAGAAA+AGG - Chr5:33917932-33917951 MsG0580026281.01.T01:intron 40.0%
! GAGAAAGTGTGTCAGGAAAA+GGG + Chr5:33918207-33918226 None:intergenic 40.0%
! GATAAGATTTGGGTTACTGC+TGG - Chr5:33919238-33919257 MsG0580026281.01.T01:intron 40.0%
! TGTTTGTCTTGTTGTCTAGC+TGG + Chr5:33916121-33916140 None:intergenic 40.0%
!! AGATTGGACTTGGTGATTCA+TGG - Chr5:33916298-33916317 MsG0580026281.01.T01:exon 40.0%
!! GAGTCAAAGGTGTTCTTACA+AGG - Chr5:33919128-33919147 MsG0580026281.01.T01:intron 40.0%
!! GTCAAAGGTGTTCTTACAAG+GGG - Chr5:33919130-33919149 MsG0580026281.01.T01:intron 40.0%
!! GTGTTTGGTTCCATTTTCAG+AGG - Chr5:33916643-33916662 MsG0580026281.01.T01:CDS 40.0%
!!! GCTTGTTTTAGTGGTTCTCA+TGG - Chr5:33917051-33917070 MsG0580026281.01.T01:intron 40.0%
AACAGGGCCATTCAAACGAA+TGG + Chr5:33917853-33917872 None:intergenic 45.0%
ACTATCAGGCCAACCCAAAT+TGG - Chr5:33916777-33916796 MsG0580026281.01.T01:intron 45.0%
CACAATGTGAAGAAACCAGC+TGG + Chr5:33918324-33918343 None:intergenic 45.0%
CTACTTACCATCTGCATGTG+AGG - Chr5:33919066-33919085 MsG0580026281.01.T01:intron 45.0%
GCAAGCTTGAAGATTGGACT+TGG - Chr5:33916288-33916307 MsG0580026281.01.T01:exon 45.0%
GGAGAAAGCTCTCACTAAAG+TGG - Chr5:33916266-33916285 MsG0580026281.01.T01:three_prime_UTR 45.0%
GGTGTATCGTAAGCATCCTT+TGG + Chr5:33919280-33919299 None:intergenic 45.0%
GTAGACAATCAGCTTACCCT+TGG + Chr5:33919050-33919069 None:intergenic 45.0%
GTGACAAGTGTGACAGTAGA+GGG - Chr5:33919205-33919224 MsG0580026281.01.T01:intron 45.0%
TCAAACTTGAGACATCGAGG+AGG - Chr5:33916728-33916747 MsG0580026281.01.T01:intron 45.0%
TTCGTTTGAATGGCCCTGTT+TGG - Chr5:33917853-33917872 MsG0580026281.01.T01:intron 45.0%
! ACATAGAAGGTGTAGGGGTT+AGG - Chr5:33919401-33919420 MsG0580026281.01.T01:five_prime_UTR 45.0%
! ATTCTCTCCGGTGCCAATTT+AGG + Chr5:33916709-33916728 None:intergenic 45.0%
! CTGCTGGTGTTAAGAAGTCA+AGG - Chr5:33919254-33919273 MsG0580026281.01.T01:intron 45.0%
! GCCAACCCAAATTGGTTTAG+AGG - Chr5:33916785-33916804 MsG0580026281.01.T01:intron 45.0%
!! ATAGTATTGGCTTGGGACCT+GGG + Chr5:33916762-33916781 None:intergenic 45.0%
!! TGGCCTGATAGTATTGGCTT+GGG + Chr5:33916769-33916788 None:intergenic 45.0%
!! TTGGCCTGATAGTATTGGCT+TGG + Chr5:33916770-33916789 None:intergenic 45.0%
!! TTGGGTTGGCCTGATAGTAT+TGG + Chr5:33916775-33916794 None:intergenic 45.0%
!! TTTTGAGGAAATAGCCCGAG+CGG + Chr5:33919001-33919020 None:intergenic 45.0%
AAGCTGACCTCACATGCAGA+TGG + Chr5:33919076-33919095 None:intergenic 50.0%
ACCTTTCTGTGGCTTACCTG+TGG + Chr5:33918948-33918967 None:intergenic 50.0%
CACTCTTCTCTAAACCCAGC+TGG - Chr5:33918306-33918325 MsG0580026281.01.T01:intron 50.0%
CCTTTCTGTGGCTTACCTGT+GGG + Chr5:33918947-33918966 None:intergenic 50.0%
CGATATGCAACCCGAGTCAA+AGG - Chr5:33919115-33919134 MsG0580026281.01.T01:intron 50.0%
GGGTGAAGTGAGAATGAGGT+AGG + Chr5:33918187-33918206 None:intergenic 50.0%
GGTCCCAAGCCAATACTATC+AGG - Chr5:33916763-33916782 MsG0580026281.01.T01:intron 50.0%
GGTGACAAGTGTGACAGTAG+AGG - Chr5:33919204-33919223 MsG0580026281.01.T01:intron 50.0%
GGTTGCATATCGCAGTACAG+AGG + Chr5:33919107-33919126 None:intergenic 50.0%
TAGCCCACCTAAATTGGCAC+CGG - Chr5:33916699-33916718 MsG0580026281.01.T01:intron 50.0%
TGCATGTGAGGTCAGCTTCA+AGG - Chr5:33919078-33919097 MsG0580026281.01.T01:intron 50.0%
! AGAGTGAACTCAGAGGTGCA+TGG + Chr5:33918292-33918311 None:intergenic 50.0%
! TCTCCGGTGCCAATTTAGGT+GGG + Chr5:33916705-33916724 None:intergenic 50.0%
!! GATAGTATTGGCTTGGGACC+TGG + Chr5:33916763-33916782 None:intergenic 50.0%
CCCACAGGTAAGCCACAGAA+AGG - Chr5:33918944-33918963 MsG0580026281.01.T01:intron 55.0%
TCTGTGGCTTACCTGTGGGA+AGG + Chr5:33918943-33918962 None:intergenic 55.0%
! CTCTCCGGTGCCAATTTAGG+TGG + Chr5:33916706-33916725 None:intergenic 55.0%
! TGCTCTGAATGCCTTCCCAC+AGG - Chr5:33918929-33918948 MsG0580026281.01.T01:intron 55.0%
! TCGAGGAGGAGTACACTCCC+AGG - Chr5:33916742-33916761 MsG0580026281.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 33916087 33919544 33916087 ID=MsG0580026281.01;Name=MsG0580026281.01
Chr5 mRNA 33916087 33919544 33916087 ID=MsG0580026281.01.T01;Parent=MsG0580026281.01;Name=MsG0580026281.01.T01;_AED=0.41;_eAED=0.41;_QI=177|1|1|1|1|1|3|214|180
Chr5 exon 33916087 33916680 33916087 ID=MsG0580026281.01.T01:exon:25426;Parent=MsG0580026281.01.T01
Chr5 exon 33917650 33917704 33917650 ID=MsG0580026281.01.T01:exon:25425;Parent=MsG0580026281.01.T01
Chr5 exon 33919260 33919544 33919260 ID=MsG0580026281.01.T01:exon:25424;Parent=MsG0580026281.01.T01
Chr5 five_prime_UTR 33919368 33919544 33919368 ID=MsG0580026281.01.T01:five_prime_utr;Parent=MsG0580026281.01.T01
Chr5 CDS 33919260 33919367 33919260 ID=MsG0580026281.01.T01:cds;Parent=MsG0580026281.01.T01
Chr5 CDS 33917650 33917704 33917650 ID=MsG0580026281.01.T01:cds;Parent=MsG0580026281.01.T01
Chr5 CDS 33916301 33916680 33916301 ID=MsG0580026281.01.T01:cds;Parent=MsG0580026281.01.T01
Chr5 three_prime_UTR 33916087 33916300 33916087 ID=MsG0580026281.01.T01:three_prime_utr;Parent=MsG0580026281.01.T01
Gene Sequence

>MsG0580026281.01.T01

ATGGAGAAAGCTCTCACTAAAGTGGCAAGCTTGAAGATTGGACTTGGTGATTCATGGATTACTAAGAAAGCTAAAGAAGAATTCACTAACATCTCTCAAGACATCAATACTTTCTCAAGCACTGTTGAAGAAAAGGCCAAATGGGTTTTCAACAAACTAAAAGGTAAGCCACAGAAAGGTTTACCAGATCTCCTCAAAGATTATAATTTACCGCTCGGGCTATTTCCTCAAAACATAATCTGTTATGAGTTTGACGAAACCAAGGGTAAGCTGATTGTCTACTTACCATCTGCATGTGAGGTCAGCTTCAAGGATTCCTCTGTACTGCGATATGCAACCCGAGTCAAAGGTGTTCTTACAAGGGGAAAGCTTTCACAAATAGAGGGAATGAAAACAAAAGTTCTTGTATGGGTTAAGGTGACAAGTGTGACAGTAGAGGGTTACAAATCTGATAAGATTTGGGTTACTGCTGGTGTTAAGAAGTCAAGGCCAAAGGATGCTTACGATACACCCCGTGATGCTATTAGAGTATCAGAATTCTGA
Protein sequence

>MsG0580026281.01.T01

MEKALTKVASLKIGLGDSWITKKAKEEFTNISQDINTFSSTVEEKAKWVFNKLKGKPQKGLPDLLKDYNLPLGLFPQNIICYEFDETKGKLIVYLPSACEVSFKDSSVLRYATRVKGVLTRGKLSQIEGMKTKVLVWVKVTSVTVEGYKSDKIWVTAGVKKSRPKDAYDTPRDAIRVSEF*